A micro-drug delivery device suitable for organoids

Abstract

This invention discloses a micro-drug delivery device suitable for organoids, comprising a worktable with a drug delivery component on the top. The drug delivery component includes a vertical plate fixedly connected to one side of the top of the worktable, a horizontal plate fixedly connected to one side of the vertical plate, a groove on the top of the horizontal plate, a slider connected to the groove, a first cylinder fixedly connected to the top of the slider, a movable seat fixedly connected to the output end of the first cylinder, an adjustment groove on the bottom of the movable seat, and multiple adjustment blocks connected to the adjustment groove. This invention solves the problem that existing organoid culture methods typically use multi-well plates, requiring drug delivery as needed to obtain reliable in vitro experimental data. However, drug delivery usually only allows for delivery to a single organoid at a time. If multiple organoids need to be operated on simultaneously, repeated drug delivery is required, leading to decreased drug delivery efficiency and reduced practicality.

Description

Technical Field

[0001] This utility model relates to the field of organoid culture technology, specifically a micro-drug delivery device suitable for organoids. Background Technology

[0002] Organoids are 3D miniature organs composed of a cluster of cells with self-renewal and self-organization capabilities in vitro. They are similar to real human tissues and organs to a certain extent. Compared with 2D cell culture, organoids have cells and spatial tissues similar to their corresponding organs and have all the genetic characteristics of the human body. Therefore, they can be cultured in vitro for a long time and simulate the function of real organs, and have broad application prospects in basic research and clinical diagnosis and treatment.

[0003] Currently, organoids are typically cultured using multi-well plates, requiring drug delivery as needed to obtain reliable in vitro experimental data. However, drug delivery usually only allows for a single organoid to be administered at a time. If multiple organoids need to be manipulated simultaneously, repeated drug delivery is required, leading to decreased drug delivery efficiency and reduced practicality. Summary of the Invention

[0004] The purpose of this invention is to provide a micro-drug delivery device suitable for organoids, in order to solve the problem mentioned in the background art that existing organoids are usually cultured using multi-well plates, and drug delivery is required as needed to obtain reliable in vitro experimental data. However, when delivering the drug, only a single organoid can usually be delivered at a time. If multiple organoids need to be operated on at the same time, repeated drug delivery is required, which leads to a decrease in drug delivery efficiency and thus a reduction in practicality.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a micro-drug delivery device suitable for organoids, comprising a worktable, wherein a drug delivery component is provided on the top of the worktable; The drug delivery component includes a vertical plate fixedly connected to one side of the top of the workbench, a horizontal plate fixedly connected to one side of the vertical plate, a groove opened at the top of the horizontal plate, a slider connected to the groove, a first cylinder fixedly connected to the top of the slider, a movable seat fixedly connected to the output end of the first cylinder, an adjustment groove opened at the bottom of the movable seat, a plurality of adjustment blocks connected to the adjustment groove, a drug delivery needle fixedly connected to the bottom of the adjustment block, and a positioning component for fixing the adjustment block. The adjustment block is slidably connected to the adjustment groove, and the slider is slidably connected to the groove.

[0006] Preferably, the positioning component includes a movable groove formed on the top of the adjusting block, a movable rod connected to the movable groove, a spring fixedly connected to the bottom of the movable rod, a hemisphere fixedly connected to the top of the movable rod, and a plurality of positioning grooves evenly arranged on the top of the adjusting groove. The movable rod is slidably connected to the movable groove, and the hemisphere is inserted into the positioning groove.

[0007] Preferably, a medicine storage box is fixedly connected to one side of the top of the workbench, a piezoelectric ceramic micropump is fixedly connected to the top of the medicine storage box, a drug extraction tube is fixedly connected to one side of the piezoelectric ceramic micropump, a drug discharge tube is fixedly connected to the other side of the piezoelectric ceramic micropump, a drug dispensing tube is fixedly connected to one side of the movable seat, a drug flow tube is fixedly connected between the drug dispensing tube and the drug administration needle, a solenoid valve is fixedly connected to the drug flow tube, and the drug extraction tube is connected to the medicine storage box.

[0008] Preferably, a motor is fixedly connected to the top of the medicine storage tank, a stirring shaft is fixedly connected to the bottom of the motor, blades are fixedly connected to the outside of the stirring shaft, and the stirring shaft is rotatably connected to the medicine storage tank.

[0009] Preferably, a feeding pipe is fixedly connected to one side of the top of the medicine storage box, and a sealing cap is screwed to the top of the feeding pipe.

[0010] Preferably, a protective groove is provided at the bottom of the movable rod, and a protective rod is fixedly connected to the bottom of the movable groove, with the protective rod slidably connected to the protective groove.

[0011] Preferably, a second cylinder is fixedly connected to one side of the horizontal plate, and the output end of the second cylinder is fixedly connected to the slider.

[0012] Compared with the prior art, the beneficial effects of this utility model are: 1. By setting up a workbench and drug delivery components, the first cylinder can push the moving seat to move vertically, which in turn can drive multiple drug delivery needles to move vertically. This allows for simultaneous drug delivery to multiple organoids, greatly improving drug delivery efficiency and reducing the frequency of drug delivery operations. At the same time, the drug delivery needles can also move back and forth to adjust the spacing, which can adapt to different perforated plates, thus greatly expanding the scope of application and significantly improving the practicality and efficiency of the device. 2. By setting up a positioning component, the hemisphere can be pushed into the positioning groove under the push of the spring, which can fix the adjusting block and thus fix the drug delivery needle. It can position the adjusted drug delivery needle and prevent it from moving randomly, affecting the stability of drug delivery, thereby further improving its practicality. Attached Figure Description

[0013] Figure 1 A three-dimensional structural schematic diagram provided for this utility model; Figure 2 Front view provided for this utility model; Figure 3 Provided by this utility model Figure 2 A three-dimensional cross-sectional view at point AA; Figure 4 Provided by this utility model Figure 3Enlarged view of point C in the middle; Figure 5 The left view provided for this utility model; Figure 6 Provided by this utility model Figure 5 A three-dimensional cross-sectional view at point BB; Figure 7 Provided by this utility model Figure 6 Enlarged view of point D in the middle.

[0014] In the diagram: 1. Workbench; 21. Vertical plate; 22. Horizontal plate; 23. Slide groove; 24. Slider; 25. First cylinder; 26. Moving seat; 27. Adjusting groove; 28. Adjusting block; 29. ​​Drug injection needle; 31. Moving groove; 32. Moving rod; 33. Spring; 34. Hemisphere; 35. Positioning groove; 41. Drug storage tank; 42. Piezoelectric ceramic micro pump; 43. Drug extraction tube; 44. Drug discharge tube; 45. Drug distribution tube; 46. Drug flow tube; 47. Solenoid valve; 51. Motor; 52. Stirring shaft; 53. Blade; 61. Feeding tube; 62. Sealing cover; 71. Protective groove; 72. Protective rod; 81. Second cylinder. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 as well as Figure 7This utility model provides a technical solution: a micro-drug delivery device suitable for organoids, including a workbench 1. A drug delivery component is provided on the top of the workbench 1. The drug delivery component includes a vertical plate 21 fixedly connected to one side of the top of the workbench 1, a horizontal plate 22 fixedly connected to one side of the vertical plate 21, a groove 23 on the top of the horizontal plate 22, a slider 24 connected to the groove 23, a first cylinder 25 fixedly connected to the top of the slider 24, a movable seat 26 fixedly connected to the output end of the first cylinder 25, an adjustment groove 27 on the bottom of the movable seat 26, multiple adjustment blocks 28 connected to the adjustment groove 27, a drug delivery needle 29 fixedly connected to the bottom of the adjustment block 28, and a positioning component for fixing the adjustment block 28. The adjustment block 28 is slidably connected to the adjustment groove 27. The slider 24 is slidably connected to the slide groove 23. The adjustment block 28 can move the drug delivery needles 29. The distance between the drug delivery needles 29 can be flexibly controlled according to the perforated plate, improving the applicability. The first cylinder 25 can push the moving seat 26 to move vertically, which can move multiple drug delivery needles 29 vertically, allowing simultaneous drug delivery to multiple organoids, greatly improving drug delivery efficiency and reducing operation frequency. A drug storage tank 41 is fixedly connected to one side of the top of the workbench 1. A piezoelectric ceramic micropump 42 is fixedly connected to the top of the drug storage tank 41. A drug extraction tube 43 is fixedly connected to one side of the piezoelectric ceramic micropump 42, and a drug discharge tube 44 is fixedly connected to the other side. A drug dispensing tube 45 is fixedly connected to one side of the moving seat 26. A drug delivery tube 46 is fixedly connected between the drug delivery tube 45 and the drug delivery needle 29. A solenoid valve 47 is fixedly connected to the drug delivery tube 46. The drug extraction tube 43 is connected to the drug storage tank 41. The piezoelectric ceramic micropump 42 can extract the drug from the drug storage tank 41 through the drug extraction tube 43, and then the drug enters the drug discharge tube 44 and the drug distribution tube 45. It can then be distributed into the drug delivery tube 46 and then enter the drug delivery needle 29, which can realize the simultaneous administration of drugs to multiple organoids in a single dose. The piezoelectric ceramic micropump 42 can be of model MP-600. It controls the deformation of the piezoelectric plate through high-frequency voltage (5-30V) to generate pulsed liquid flow with stable flow rate. With the help of the solenoid valve 47, it can realize micro-dose drug delivery. A motor 51 is fixedly connected to the top of the drug storage tank 41, and a stirrer is fixedly connected to the bottom of the motor 51. A stirring shaft 52 has blades 53 fixedly connected to its outer side. The stirring shaft 52 is rotatably connected to the medicine storage tank 41. When the motor 51 operates, it drives the stirring shaft 52 to rotate, which in turn drives the blades 53 to rotate, allowing for rapid and even mixing of the medicine in the medicine storage tank 41. A feeding pipe 61 is fixedly connected to one side of the top of the medicine storage tank 41, and a sealing cap 62 is screwed to the top of the feeding pipe 61. Medicine can be added to the medicine storage tank 41 through the feeding pipe 61, and the sealing cap 62 can close the feeding pipe 61. A second cylinder 81 is fixedly connected to one side of the horizontal plate 22. The output end of the second cylinder 81 is fixedly connected to the slider 24. When the second cylinder 81 operates, it pushes the slider 24 to move left and right, which in turn drives the administration needle 29 to move left and right.This can further expand the drug delivery range.

[0017] Please see Figure 1 , Figure 2 , Figure 3 as well as Figure 4 The positioning components include a movable groove 31 on the top of the adjusting block 28, a movable rod 32 connected to the movable groove 31, a spring 33 fixedly connected to the bottom of the movable rod 32, a hemisphere 34 fixedly connected to the top of the movable rod 32, and multiple positioning grooves 35 evenly arranged on the top of the adjusting groove 27. The movable rod 32 is slidably connected to the movable groove 31, the hemisphere 34 is inserted into the positioning groove 35, and the bottom of the spring 33 is fixedly connected to the bottom of the movable groove 31. When the adjusting block 28 moves, the hemisphere 34 can disengage from the positioning groove 35, and the movement of the hemisphere 34 can drive the movable rod 32 to compress the spring 35. 3. The deformation of the spring 33 and the reset of the spring 33 can drive the hemisphere 34 to reset, so that it can be re-locked into other positioning slots 35, which can fix the adjusting block 28, and then fix the drug administration needle 29 to prevent it from moving, prevent the wrong drug from being added, and improve the stability of drug administration. The bottom of the moving rod 32 is provided with a protective groove 71, and a protective rod 72 is fixedly connected to the bottom of the moving groove 31. The protective rod 72 is slidably connected to the protective groove 71. When the moving rod 32 moves, the protective rod 72 can slide in the protective groove 71, which can prevent the moving rod 32 from rotating, and thus protect the spring 33 to prevent it from rotating and being damaged.

[0018] Working principle: During operation, pushing the adjusting block 28 moves the hemisphere 34 out of the current positioning slot 35. The movement of the hemisphere 34 causes the moving rod 32 to move downward, which in turn compresses the spring 33, causing it to deform. After the spring 33 returns to its original position, it causes the hemisphere 34 to return to its original position and re-enter another positioning slot 35. This fixes the adjusting block 28 and the drug delivery needle 29. The spacing of the drug delivery needle 29 can be adjusted as needed. Then, the medicine is added to the medicine storage tank 41. Starting the motor 51 drives the stirring shaft 52 and the blades 53 to rotate, which can quickly mix the medicine. Then, the perforated plate is placed on top of the worktable 1, and starting the first cylinder 25 pushes the moving seat 26 downward. This causes the drug delivery needle 29 to move downwards, and the piezoelectric ceramic micropump 42 to start. The drug can be drawn out of the drug storage tank 41 through the drug extraction tube 43, and then enters the drug discharge tube 44 and the drug distribution tube 45. It can then be distributed into the drug flow tube 46 and then enter the drug delivery needle 29, enabling the simultaneous administration of drugs to multiple organoids in a single operation. The first cylinder 25 can drive the moving seat 26 and the drug delivery needle 29 to move upwards. The second cylinder 81 can push the slider 24 to move laterally, which in turn drives the drug delivery needle 29 to move laterally. This allows the drug delivery operation to be performed on multiple organoids in sequence. The above is the working process of the entire device. All contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0019] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A micro-drug delivery device suitable for organoids, comprising a worktable (1), characterized in that: The workbench (1) is equipped with a drug delivery component on its top; The drug delivery component includes a vertical plate (21) fixedly connected to one side of the top of the workbench (1), a horizontal plate (22) fixedly connected to one side of the vertical plate (21), a slide groove (23) opened on the top of the horizontal plate (22), a slider (24) connected to the slide groove (23), a first cylinder (25) fixedly connected to the top of the slider (24), a movable seat (26) fixedly connected to the output end of the first cylinder (25), an adjustment groove (27) opened at the bottom of the movable seat (26), a plurality of adjustment blocks (28) connected to the adjustment groove (27), a drug delivery needle (29) fixedly connected to the bottom of the adjustment block (28), and a positioning component for fixing the adjustment block (28). The adjustment block (28) is slidably connected to the adjustment groove (27), and the slider (24) is slidably connected to the slide groove (23).

2. The micro-drug delivery device suitable for organoids according to claim 1, characterized in that: The positioning component includes a movable groove (31) opened on the top of the adjusting block (28), a movable rod (32) connected to the movable groove (31), a spring (33) fixedly connected to the bottom of the movable rod (32), a hemisphere (34) fixedly connected to the top of the movable rod (32), and a plurality of positioning grooves (35) evenly arranged on the top of the adjusting groove (27). The movable rod (32) is slidably connected to the movable groove (31), and the hemisphere (34) is inserted into the positioning groove (35).

3. A micro-drug delivery device suitable for organoids according to claim 1, characterized in that: A medicine storage tank (41) is fixedly connected to one side of the top of the workbench (1). A piezoelectric ceramic micropump (42) is fixedly connected to the top of the medicine storage tank (41). A drug extraction tube (43) is fixedly connected to one side of the piezoelectric ceramic micropump (42). A drug discharge tube (44) is fixedly connected to the other side of the piezoelectric ceramic micropump (42). A drug dispensing tube (45) is fixedly connected to one side of the movable seat (26). A drug flow tube (46) is fixedly connected between the drug dispensing tube (45) and the drug administration needle (29). A solenoid valve (47) is fixedly connected to the drug flow tube (46). The drug extraction tube (43) is connected to the medicine storage tank (41).

4. A micro-drug delivery device suitable for organoids according to claim 3, characterized in that: The top of the medicine storage box (41) is fixedly connected to a motor (51), the bottom of the motor (51) is fixedly connected to a stirring shaft (52), the outside of the stirring shaft (52) is fixedly connected to a blade (53), and the stirring shaft (52) is rotatably connected to the medicine storage box (41).

5. A micro-drug delivery device suitable for organoids according to claim 3, characterized in that: A feeding pipe (61) is fixedly connected to one side of the top of the medicine storage box (41), and a sealing cap (62) is screwed to the top of the feeding pipe (61).

6. A micro-drug delivery device suitable for organoids according to claim 2, characterized in that: The bottom of the movable rod (32) is provided with a protective groove (71), and the protective rod (72) is fixedly connected to the bottom of the movable groove (31). The protective rod (72) is slidably connected to the protective groove (71).

7. A micro-drug delivery device suitable for organoids according to claim 1, characterized in that: A second cylinder (81) is fixedly connected to one side of the horizontal plate (22), and the output end of the second cylinder (81) is fixedly connected to the slider (24).

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