A drip staining apparatus for HE staining

Abstract

This utility model discloses a drop-dye HE dyeing device, including a dyeing mounting frame with a liquid injection mechanism and a waste liquid recovery mechanism. The liquid injection mechanism includes a liquid injection horizontal movement module, an liquid injection lifting module and a needle cleaning assembly at the output end of the liquid injection horizontal movement module, and a liquid injection needle at the output end of the liquid injection lifting module. One end of the liquid injection needle is externally connected to a liquid injection pump. The liquid injection horizontal movement module and the liquid injection lifting module can drive the liquid injection needle to extend into each material trough of the material bin. The needle cleaning assembly is used to clean the liquid injection needle. The waste liquid recovery mechanism includes a recovery horizontal movement module, a recovery lifting module at the output end of the recovery horizontal movement module, and a liquid extraction needle at the output end of the recovery lifting module. The liquid extraction needle is externally connected to a negative pressure suction device. This utility model can realize the full automation of liquid injection, liquid extraction and cleaning, significantly improving efficiency and reducing manual labor intensity.

Description

Technical Field

[0001] This utility model relates to a drop staining HE staining device, which belongs to pathological technology and is applied in biomedical disciplines such as pathology, histology and embryology, and clinical laboratory diagnosis. Background Technology

[0002] Staining is of great significance in the diagnosis of pathological tissue morphology, scientific experimental research, and teaching. Without staining tissue sections, the internal structure of the tissue cannot be seen, and the cell nuclei cannot be identified. Therefore, researchers cannot provide a basis for judgment to determine whether the tissue is abnormal.

[0003] HE staining uses hematoxylin and eosin to stain the nucleus and cytoplasm, respectively, to distinguish the morphology of normal cells. In practical applications, it can identify abnormal pathological changes such as tissue cell necrosis, edema, degeneration, and inflammatory cell infiltration.

[0004] Current technology for staining tissue sections largely relies on manual operation. Operators manually draw the staining solution and inject it into the hopper containing the tissue sections, ensuring thorough contact between the solution and the sections to achieve staining. Since multiple staining solutions are typically used, after each solution is applied, operators must manually remove any residual solution from the hopper before injecting new solutions. This method is inefficient and labor-intensive, making the replacement of manual labor with machinery an inevitable trend for industry transformation and upgrading. Therefore, there is an urgent need for equipment capable of automatically staining tissue sections, requiring the design of a device that automatically injects and extracts solutions from the hopper. Utility Model Content

[0005] To address some or all of the problems existing in the prior art, this utility model provides a drop-dye HE staining device for use on an automatic staining machine. The automatic staining machine includes a machine base with a material hopper channel for conveying a material hopper containing tissue sections. The upper end of the material hopper is provided with a material trough for placing the tissue sections. The device is characterized in that: the drop-dye HE staining device includes a staining mounting frame connected to the machine base. The staining mounting frame is provided with an injection mechanism and a waste liquid recovery mechanism respectively connected to the material hopper channel. The injection mechanism injects a solution into the material trough, and the waste liquid recovery mechanism extracts waste liquid from the material trough. The injection mechanism includes an injection transverse movement module connected to the staining mounting frame, and an injection lifting mounting plate is provided on the output end of the injection transverse movement module. The liquid injection lifting mounting plate is equipped with a liquid injection lifting module and a needle cleaning assembly. The output end of the liquid injection lifting module is equipped with a liquid injection needle, one end of which is connected to a liquid injection pump. The liquid injection horizontal movement module and the liquid injection lifting module can drive the liquid injection needle to extend into each material trough of the hopper. The liquid injection lifting module can also drive the liquid injection needle to connect with the needle cleaning assembly, which is used to clean the liquid injection needle. The waste liquid recovery mechanism includes a recovery horizontal movement module, which is connected to the dyeing mounting frame. The output end of the recovery horizontal movement module is equipped with a recovery lifting module, and the output end of the recovery lifting module is equipped with a liquid extraction needle. The liquid extraction needle is connected to a negative pressure suction device. The recovery horizontal movement module and the recovery lifting module can drive the liquid extraction needle to extend into each material trough of the hopper.

[0006] As a further improvement of this utility model, the liquid injection transverse movement module includes a liquid injection transverse movement fixing seat, which is connected to the dyeing mounting frame. The liquid injection transverse movement fixing seat is provided with a lead screw assembly and a liquid injection transverse movement motor. The output end of the liquid injection transverse movement motor is connected to the lead screw assembly. A liquid injection transverse movement slider is slidably provided on the liquid injection transverse movement fixing seat. The liquid injection transverse movement slider is connected to the lead screw assembly. The liquid injection lifting mounting plate is connected to the liquid injection transverse movement slider.

[0007] As a further improvement of this utility model, the liquid injection lifting module includes a liquid injection lifting motor, which is connected to the liquid injection lifting mounting plate. The liquid injection lifting mounting plate is provided with a liquid injection lifting driven wheel, and the output end of the liquid injection lifting motor is provided with a liquid injection lifting driving wheel. A liquid injection lifting transmission belt is sleeved on the liquid injection lifting driving wheel and the liquid injection lifting driven wheel, and a liquid injection lifting slider is provided on the liquid injection lifting transmission belt. The liquid injection needle is connected to the liquid injection lifting slider.

[0008] As a further improvement of this utility model, the liquid injection lifting mounting plate is provided with a positioning sensor, and the liquid injection lifting slider is provided with a detection lever, which can be connected to the positioning sensor.

[0009] As a further improvement of this utility model, the syringe cleaning assembly includes a cleaning mounting frame, which is connected to the liquid injection lifting mounting plate. The cleaning mounting frame is provided with a cleaning tank, and the cleaning tank has a needle hole that passes through the upper and lower end faces of the cleaning tank. The liquid injection needle can pass through the needle hole and is in clearance fit with the needle hole. The side wall of the cleaning tank is provided with a water inlet connector and a water outlet connector, which are respectively connected to the needle hole. The water inlet connector is connected to the cleaning liquid, and the water outlet connector is connected to the negative pressure suction device.

[0010] As a further improvement of this utility model, the lower end face of the injection needle is a sealed structure, and the liquid outlet of the injection needle is located on the side wall of the injection needle.

[0011] As a further improvement of this utility model, the recycling lifting module includes a recycling lifting mounting plate, on which a recycling lifting motor and a rotatable recycling lifting driven wheel are provided. A recycling lifting drive wheel is provided on the output end of the recycling lifting motor. A recycling lifting transmission belt is sleeved on the recycling lifting drive wheel and the recycling lifting driven wheel. A recycling lifting slider is provided on the recycling lifting transmission belt. The liquid extraction needle is connected to the recycling lifting slider.

[0012] As a further improvement of this utility model, the recovery lifting slider is provided with a avoidance rotary motor, and the output end of the avoidance rotary motor is provided with a rotary mounting block. The avoidance rotary motor can drive the rotary mounting block to rotate, and the liquid suction needle is connected to the rotary mounting block.

[0013] As a further improvement of this utility model, the recovery lifting slider is provided with a first sensor, and the rotating mounting block is provided with a first sensing lever, which can be connected to the first sensor.

[0014] As a further improvement of this utility model, there are two liquid extraction needles, and the distance between the two liquid extraction needles is less than the length of the material trough.

[0015] Compared with the prior art, the beneficial effects of this utility model are: This invention, through the cooperation of an injection mechanism and a waste liquid recovery mechanism, enables full automation of the injection, extraction, and cleaning processes, significantly improving efficiency and reducing manual labor intensity. Its modular design results in a simple structure, fast response, and convenient control; it also allows for easy addition, reduction, or adjustment of the number of processing stations according to actual process requirements, enhancing the equipment's flexibility and scalability. Attached Figure Description

[0016] To more clearly illustrate the solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the structure of the silo in an embodiment of this utility model; Figure 2 This is a schematic diagram of the overall structure of an embodiment of the present utility model; Figure 3 This is a schematic diagram of the structure of the liquid injection transverse movement module in an embodiment of this utility model; Figure 4 This is a schematic diagram of the structure of the liquid injection lifting module in an embodiment of this utility model; Figure 5 This is a schematic diagram of the liquid injection lifting module from another perspective in an embodiment of this utility model; Figure 6 This is a schematic diagram of the structure of the syringe cleaning assembly in an embodiment of this utility model; Figure 7 This is a schematic diagram of the structure of the recovery lifting module in an embodiment of this utility model; Figure 8 This is a schematic diagram of the recovery lifting module from another perspective in an embodiment of this utility model; Figure 9 This is a schematic diagram of the air blowing assembly in an embodiment of this utility model. Detailed Implementation

[0018] Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains; the terminology used in the specification is for the purpose of describing particular embodiments only and is not intended to limit the invention; the terms "comprising" and "having," and any variations thereof, in the specification, claims, and accompanying drawings of this invention are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the specification, claims, or accompanying drawings of this invention are used to distinguish different objects, not to describe a particular order.

[0019] In this invention, the reference to "embodiment" means that a specific feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this invention. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment to other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described in this invention can be combined with other embodiments.

[0020] To enable those skilled in the art to better understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.

[0021] like Figure 1-9 As shown, a drop staining HE staining device is used to install on an automatic staining machine. The automatic staining machine includes a machine base 100, on which a material hopper channel 200 is provided. The material hopper channel 200 is used to transport a material hopper 300 containing tissue sections. The upstream processing position of the automatic staining machine transports the material hopper 300 containing tissue sections to the material hopper channel 200, and then transports it to the processing position of the drop staining HE staining device through the material hopper channel 200. The function of this drop staining HE staining device is to inject the chemical solution into each material tank 400 containing tissue sections within the material tank 300, so that the chemical solution fully contacts the tissue sections in the material tank 400 to achieve staining; and after staining, the waste liquid in the material tank 400 is pumped out to allow for the injection of different chemical solutions into the material tank 400.

[0022] The HE staining apparatus includes a staining mounting frame 1, which is fixedly connected to the machine base 100. The staining mounting frame 1 is equipped with a liquid injection mechanism 500 and a waste liquid recovery mechanism 600, both connected to a material hopper channel 200. The liquid injection mechanism 500 injects the chemical solution into each trough within the material hopper on the material hopper channel 200, and the waste liquid recovery mechanism 600 extracts the waste liquid from each trough within the material hopper on the material hopper channel 200. In operation, the material hopper containing tissue sections is moved to the processing position of the HE staining apparatus via the material hopper channel 200. Then, the liquid injection mechanism 500 injects the chemical solution into each trough within the material hopper, ensuring sufficient contact between the chemical solution and the tissue sections in the trough for staining. After staining is complete, the waste liquid recovery mechanism 600 is activated to extract the stained waste liquid from the trough. Repeat the above process until all tissue sections in all tanks have been stained and all staining waste liquid has been removed; then transfer the hopper to the downstream processing position of the automatic staining machine through the hopper channel 200.

[0023] The injection mechanism 500 includes an injection transverse module 2, which is fixedly connected to the staining mounting frame 1. An injection lifting mounting plate 3 is provided on the output end of the injection transverse module 2. An injection lifting module 4 and a needle cleaning assembly 5 are provided on the injection lifting mounting plate 3. An injection needle 510 is provided on the output end of the injection lifting module 4, with one end of the injection needle 510 externally connected to an injection pump. The injection transverse module 2 drives the injection needle 510 to move along the length of the hopper, allowing the injection needle 510 to align with each material trough in the hopper. The injection lifting module 4 drives the injection needle 510 to descend or ascend. Through the cooperation of the injection transverse module 2 and the injection lifting module 4, the injection needle 510 can be driven to extend into each material trough of the hopper, facilitating the staining of tissue sections in each trough. The injection needle 510 injects the drug solution into the trough via the external injection pump, allowing the drug solution to stain the tissue sections in the hopper. The injection lifting module 4 can also drive the injection needle 510 to connect with the needle cleaning assembly 5. The needle cleaning assembly 5 is used to clean the injection needle 510. By cleaning the injection needle 510, different medications can be prevented from affecting each other, so that the injection mechanism 500 can be used for injection of different medications, thus improving the versatility of the equipment.

[0024] like Figure 3 As shown, the liquid injection transverse movement module 2 includes a liquid injection transverse movement fixed base 21, which is connected to the dyeing mounting frame 1. The liquid injection transverse movement fixed base 21 is equipped with a lead screw assembly (not shown) and a liquid injection transverse movement motor 22. The output end of the liquid injection transverse movement motor 22 is connected to the lead screw assembly. A liquid injection transverse movement slider 23 is slidably mounted on the liquid injection transverse movement fixed base 21 and is connected to the lead screw assembly. During operation, the lead screw assembly is driven to rotate by the liquid injection transverse movement motor 22, which in turn drives the liquid injection transverse movement slider 23 to slide laterally on the liquid injection transverse movement fixed base 21. The liquid injection transverse movement slider 23 drives the liquid injection lifting mounting plate 3, the liquid injection lifting module 4, and the liquid injection needle 510 to move synchronously, thereby moving the liquid injection needle 510 to directly above each material trough in the material bin.

[0025] like Figure 4-5 As shown, the liquid injection lifting module 4 includes a liquid injection lifting motor 41, which is fixedly connected to the liquid injection lifting mounting plate 3. The liquid injection lifting mounting plate 3 is equipped with a liquid injection lifting driven wheel 42, and the output end of the liquid injection lifting motor 41 is equipped with a liquid injection lifting drive wheel 43. A liquid injection lifting transmission belt 44 is fitted onto the liquid injection lifting drive wheel 43 and the liquid injection lifting driven wheel 42. A liquid injection lifting slider 45 is mounted on the liquid injection lifting transmission belt 44, and the liquid injection needle 510 is connected to the liquid injection lifting slider 45. During operation, the liquid injection lifting motor 41 drives the liquid injection lifting drive wheel 43 to rotate, which in turn drives the liquid injection lifting transmission belt 44 to move. The liquid injection lifting transmission belt 44 then drives the liquid injection lifting slider 45 and the liquid injection needle 510 to move synchronously, causing the liquid injection needle 510 to extend into or out of the material trough in the hopper.

[0026] To limit and guide the lifting movement of the injection needle 510, an injection lifting guide rail 46 is provided on the injection lifting mounting plate 3, and the injection lifting slider 45 is slidably engaged with the injection lifting guide rail 46. When the injection lifting motor 41 is working, it drives the injection lifting slider 45 to move; the injection lifting slider 45 is constrained by the injection lifting guide rail 46 and can only slide along the direction of the injection lifting guide rail 46, thereby driving the injection needle 510 to be accurately inserted into the material tank to complete the injection.

[0027] To improve control accuracy, a positioning sensor 47 is installed on the liquid injection lifting mounting plate 3, and a detection lever 48 is installed on the liquid injection lifting slider 45. The detection lever 48 can connect with the positioning sensor 47. Normally, the detection lever 48 and the positioning sensor 47 are separate. When liquid injection is needed, the liquid injection lifting motor 41 drives the liquid injection lifting slider 45 to descend, causing the liquid injection needle 510 to descend synchronously. As the liquid injection lifting slider 45 descends, the detection lever 48 on the slider 45 connects with the positioning sensor 47. At this point, the liquid injection needle 510 is precisely inserted into the material trough of the hopper. The positioning sensor 47 sends a signal to the controller of the automatic dyeing machine, which immediately stops the liquid injection lifting motor 41. Through the cooperation of the detection lever 48 and the positioning sensor 47, the liquid injection needle 510 can be accurately inserted into the material trough, avoiding the problem of inserting too much and breaking the needle or puncturing the hopper, and also preventing inaccurate liquid injection due to incomplete insertion.

[0028] To facilitate cleaning of the injection needle 510, in this embodiment, the lower end face of the injection needle 510 is a sealed structure, and the outlet hole of the injection needle 510 is located on the side wall of the injection needle 510. Placing the outlet hole on the side wall of the injection needle 510 prevents the liquid from dripping when the injection needle 510 is raised, avoiding contamination of adjacent material tanks or equipment and improving operational cleanliness. Furthermore, during cleaning, cleaning fluid can be injected into the interior of the injection needle 510 through the outlet hole, thereby cleaning the interior of the injection needle 510.

[0029] Specifically, such as Figure 6 As shown, the syringe cleaning assembly 5 includes a cleaning mounting frame 51, which is fixedly connected to the liquid injection lifting mounting plate 3. A cleaning tank 52 is mounted on the cleaning mounting frame 51, and the cleaning tank 52 is made of a flexible material (such as silicone). The cleaning tank 52 has a needle hole 53 at its center, penetrating both the upper and lower end faces. The liquid injection needle 510 can pass through the needle hole 53 and is fitted with a clearance between the needle hole 53 and the needle. During liquid injection, the liquid injection needle 510 passes through the needle hole 53 and extends into the material trough inside the hopper. The side wall of the cleaning tank 52 is provided with a water inlet connector 54 and a water outlet connector 55, both of which are connected to the needle hole 53. The water inlet connector 54 is connected to the cleaning liquid, and the water outlet connector 55 is connected to a negative pressure suction device.

[0030] When cleaning is required, the liquid injection horizontal movement module 2 and the liquid injection lifting module 4 work together to insert the liquid injection needle 510 into the needle hole 53 of the cleaning tank 52. Cleaning fluid is injected into the cleaning tank 52 through the water inlet connector 54. The cleaning fluid flows over the outer wall of the liquid injection needle 510, cleaning its outer wall. Simultaneously, because the liquid outlet of the liquid injection needle 510 is on the side wall, some cleaning fluid flows into the interior of the liquid injection needle 510 through the outlet, cleaning its interior. At the same time, the water outlet connector 55 generates negative pressure suction through an external negative pressure suction device, which can suck away the waste liquid generated during cleaning in the cleaning tank 52, preventing cleaning fluid overflow.

[0031] During operation, the upstream processing station of the automatic staining machine transports the hopper containing tissue sections to the hopper flow channel 200, and then transports the hopper containing tissue sections to the injection station via the hopper flow channel 200. The injection transverse motor 22 is controlled to operate, driving the injection transverse slider 23 to move via the lead screw assembly, aligning the injection needle 510 with one of the troughs in the hopper. The injection lifting motor 41 drives the injection lifting slider 45 downwards via a transmission belt, causing the injection needle 510 to insert into the trough. The injection pump starts, and the solution is injected into the trough from the outlet hole on the side wall of the injection needle 510; after injection, the injection lifting module 4 raises the injection needle 510. This process is repeated until all troughs containing tissue sections in the hopper have been injected; afterwards, the solution will fully contact the tissue sections in the troughs, achieving the purpose of staining the tissue sections in the hopper.

[0032] The waste liquid recovery mechanism 600 includes a recovery transverse module 6, which is fixedly connected to the dyeing mounting frame 1. The output end of the recovery transverse module 6 is provided with a recovery lifting module 7, and the output end of the recovery lifting module 7 is provided with a liquid extraction needle 610 and an air blowing assembly 8. The liquid extraction needle 610 is externally connected to a negative pressure suction device, and the air blowing assembly 8 is externally connected to an air source. During processing, after the drug solution in the material tank has stained the tissue section, the retrieval horizontal movement module 6 and the retrieval lifting module 7 work together to drive the liquid extraction needle 610 to be inserted into the corresponding material tank. The negative pressure generated by the negative pressure suction device connected to the liquid extraction needle 610 draws away the waste liquid in the material tank. At the same time, the retrieval horizontal movement module 6 and the retrieval lifting module 7 work together to drive the air blowing component 8 to move directly above each material tank in the material box flow channel. The air blowing component 8 blows air into the material tank to form a directional airflow, which drives the waste liquid remaining on the side wall of the material tank to gather towards the liquid extraction needle 610. This allows the liquid extraction needle 610 to completely remove the waste liquid from the material tank, effectively solving the problem of incomplete suction caused by liquid surface tension or liquid adhesion.

[0033] The structure of the recovery transverse module 6 is the same as that of the liquid injection transverse module 2, so the specific structure of the recovery transverse module 6 will not be described in detail in this article.

[0034] like Figure 7-8 As shown, the recycling lifting module 7 includes a recycling lifting mounting plate 71, which is connected to the output end of the recycling horizontal movement module 6. A recycling lifting motor 72 and a freely rotatable recycling lifting driven wheel 73 are mounted on the recycling lifting mounting plate 71. A recycling lifting drive wheel 74 is mounted on the output shaft of the recycling lifting motor 72, and a recycling lifting transmission belt 75 is fitted onto the recycling lifting drive wheel 74 and the recycling lifting driven wheel 73. A recycling lifting slider 76 is fixedly mounted on the recycling lifting transmission belt 75; a liquid extraction needle 610 and an air blowing assembly 8 are respectively connected to the recycling lifting slider 76. When the recycling horizontal movement module 6 drives the air blowing assembly 8 and the liquid extraction needle 610 to move directly above the material trough, the recycling lifting motor 72 is controlled to operate. The recycling lifting motor 72 drives the recycling lifting slider 76 to move vertically via belt drive, thereby causing the liquid extraction needle 610 to insert into the corresponding material trough, facilitating the extraction of waste liquid from the material trough; simultaneously, the air blowing assembly 8 moves above the material trough, facilitating the blowing of air into the material trough.

[0035] To limit and guide the direction of the lifting motion, a retrieval lifting guide rail 77 is fixedly installed on the retrieval lifting mounting plate 71, and the retrieval lifting slider 76 is slidably engaged with the retrieval lifting guide rail 77. When the retrieval lifting motor 72 is working, it can drive the retrieval lifting slider 76 to slide on the retrieval lifting guide rail 77. Through the sliding engagement between the retrieval lifting slider 76 and the retrieval lifting guide rail 77, the lifting direction of the suction needle 610 can be limited and guided to ensure smooth lifting motion.

[0036] To position the descent stroke of the suction needle 610, a second sensor 78 is installed on the recovery lifting mounting plate 71, and a second sensing tab 79 is installed on the recovery lifting slider 76. The second sensing tab 79 can connect with the second sensor 78. Normally, the second sensing tab 79 and the second sensor 78 are separated. When waste liquid needs to be recovered, the recovery lifting motor 72 drives the recovery lifting slider 76 to descend, causing the suction needle 610 and the air blowing assembly 8 to descend synchronously. As the recovery lifting slider 76 descends, the second sensing tab 79 on the recovery lifting slider 76 connects with the second sensor 78. At this time, the suction needle 610 is inserted into the material trough of the hopper, and the air blowing assembly 8 moves to the air blowing position above the hopper. The second sensor 78 will send a feedback signal to the control center of the automatic dyeing machine. The control center will then immediately control the recovery lifting motor 72 to stop working. The cooperation between the second sensing lever 79 and the second sensor 78 enables the liquid extraction needle 610 to be accurately inserted into the material trough, while simultaneously driving the air blowing assembly 8 to the air blowing position, thereby improving the stability of the processing and the accuracy of the control.

[0037] In actual operation, when the recovery transverse module 6 drives the liquid extraction needle 610 and the air blowing assembly 8 to move along the length of the material box, they may overlap with the position of the liquid injection mechanism 500. Therefore, to avoid motion interference, a avoidance rotary motor 9 is installed on the recovery lifting slider 76. A rotary mounting block 10 is connected to the output end of the avoidance rotary motor 9, and the liquid extraction needle 610 and the air blowing assembly 8 are respectively connected to the rotary mounting block 10. During processing, if the liquid extraction needle 610 and the air blowing assembly 8 overlap with the position of the liquid injection needle 510 on the liquid injection mechanism 500, the rotary mounting block 10 can be rotated 90° in the horizontal plane by the avoidance rotary motor 9; this will cause the liquid extraction needle 610 and the air blowing assembly 8 to rotate synchronously, thereby moving the position of the liquid extraction needle 610 and the air blowing assembly 8 away from directly above the material box, achieving avoidance and solving the problem of motion interference.

[0038] To improve the accuracy of the avoidance mechanism, a first sensor 11 is installed on the recovery lifting slider 76, and a first sensing lever 12 is fixed on the rotating mounting block 10. Normally, the first sensing lever 12 and the first sensor 11 are separate. When avoidance rotation is required, the avoidance rotation motor 9 drives the rotating mounting block 10 to rotate, causing the first sensing lever 12 to move together until it engages with the first sensor 11. The first sensor 11 then sends a feedback signal to stop the avoidance rotation motor 9. At this point, the rotating mounting block 10 has rotated 90°, precisely causing the suction needle 610 and the air blowing assembly 8 to rotate to a position misaligned with the injection needle 510 of the injection mechanism 500, thus achieving the avoidance function.

[0039] like Figure 9 As shown, the air blowing assembly 8 includes an air nozzle 81, which is fixedly mounted on the rotating mounting block 10. One end of the air nozzle 81 is connected to an external air source through a pipeline, and the other end is provided with a triangular air blowing part 82. Multiple air blowing holes 83 are provided on the end face of the air blowing part 82. During operation, the external air source delivers high-pressure gas to the air nozzle 81. The gas flows through the air nozzle 81 into each air blowing hole 83, and then blows into each material tank through each air blowing hole 83, blowing the liquid medicine in the material tank toward the liquid extraction needle 610 so that the liquid extraction needle 610 can extract all the liquid medicine.

[0040] In this embodiment, there are two extraction needles 610, which are symmetrically distributed on both sides of the air nozzle 81. The distance between the two extraction needles 610 is less than the length of the material trough, allowing both extraction needles 610 to be inserted into the same material trough simultaneously. Furthermore, by placing the two extraction needles 610 on both sides of the air nozzle 81, when the air nozzle 81 blows air into the material trough, the liquid in the trough will flow to both sides and onto the corresponding extraction needles 610. By using two extraction needles 610, the extraction efficiency can be improved, thereby increasing the processing efficiency.

[0041] During operation, once dyeing is completed in a particular tank, the recovery lateral movement module 6 is activated, causing the extraction needle 610 and air nozzle 81 to move directly above that tank. Subsequently, the recovery lifting motor 72 drives the recovery lifting slider 76 downwards via belt drive, lowering the extraction needle 610 and air nozzle 81 to a preset position. The negative pressure suction device is activated, drawing waste liquid through the extraction needle 610; simultaneously, the air source supplies air to the air nozzle 81, and the airflow is blown out from the air blowing hole 83, agitating the waste liquid and causing it to converge towards the extraction needles 610 on both sides, ensuring thorough suction. After suction is complete, the recovery lifting module 7 rises, and the recovery lateral movement module 6 moves to the next tank position, repeating the above process until all tanks are processed.

[0042] During the process of pumping waste liquid, if it is necessary to avoid the injection needle 510 of the injection mechanism 500, the avoidance rotary motor 9 is controlled to drive the rotary mounting block 10 to rotate, so that the pumping needle 610 and the air nozzle 81 are deviated from the upper part of the silo flow channel 200 to achieve avoidance.

[0043] Working principle: 1. Injection process: After the material hopper flow channel 200 sends the material hopper into the injection station, the injection transverse module 2 drives the injection needle 510 to move above the target material trough; the injection lifting module 4 drives the injection needle 510 to descend until the position sensor 47 triggers; the injection pump starts, and the liquid is injected into the material trough from the liquid outlet hole on the side wall of the injection needle 510; after the injection is completed, the injection needle 510 rises, and the transverse module moves to the next material trough to repeat the injection.

[0044] 2. Cleaning process: After a certain medicine solution is injected, the injection needle 510 is moved to the needle hole 53 of the cleaning tank 52 by the injection lifting module 4; the cleaning solution is injected from the water inlet connector 54 to clean the inside and outside of the injection needle 510; the waste liquid is sucked out from the water outlet connector 55.

[0045] 3. Waste liquid recovery process: After dyeing is completed, the recovery transverse module 6 drives the liquid extraction needle 610 and the air nozzle 81 to the top of the target material tank; the recovery lifting module 7 drives it to descend to the second sensor 78 trigger; the negative pressure suction device is started, and the waste liquid is extracted through the liquid extraction needle 610; at the same time, the air source supplies air, and the airflow is blown out from the air blowing hole 83 of the air nozzle 81, guiding the waste liquid to the liquid extraction needle 610; after the extraction is completed, the liquid extraction needle 610 and the air nozzle 81 are raised and moved to the next material tank.

[0046] 4. Avoidance process: When the positions of the suction needle 610 and the injection needle 510 conflict, the avoidance rotary motor 9 drives the rotary mounting block 10 to rotate 90°, which drives the suction needle 610 and the air nozzle 81 to rotate synchronously to achieve avoidance.

[0047] The above-described specific embodiments are preferred embodiments of this utility model, and are not intended to limit the specific scope of this utility model. The scope of this utility model includes but is not limited to the specific embodiments described above. All equivalent changes made in accordance with this utility model are within the protection scope of this utility model.

Claims

1. A drop staining HE staining apparatus for use on an automatic staining machine, the automatic staining machine comprising a machine base, a material hopper channel provided on the machine base for conveying a material hopper containing tissue sections, the material hopper having a material tray for placing tissue sections on its upper end surface, characterized in that: The HE dyeing device includes a dyeing mounting frame connected to the machine base. The dyeing mounting frame is equipped with a liquid injection mechanism and a waste liquid recovery mechanism respectively connected to the material hopper channel. The liquid injection mechanism is used to inject the chemical solution into the material tank, and the waste liquid recovery mechanism is used to extract the waste liquid in the material tank. The liquid injection mechanism includes a liquid injection transverse movement module, which is connected to the dyeing mounting frame. The output end of the liquid injection transverse movement module is provided with a liquid injection lifting mounting plate. The liquid injection lifting mounting plate is provided with a liquid injection lifting module and a needle cleaning assembly. The output end of the liquid injection lifting module is provided with a liquid injection needle. One end of the liquid injection needle is externally connected to a liquid injection pump. The liquid injection transverse movement module and the liquid injection lifting module can drive the liquid injection needle to extend into each material trough of the material bin. The liquid injection lifting module can also drive the liquid injection needle to connect with the needle cleaning assembly, which is used to clean the liquid injection needle. The waste liquid recovery mechanism includes a recovery transverse module, which is connected to the dyeing mounting frame. The output end of the recovery transverse module is equipped with a recovery lifting module, and the output end of the recovery lifting module is equipped with a liquid extraction needle. The liquid extraction needle is externally connected to a negative pressure suction device. The recovery transverse module and the recovery lifting module can drive the liquid extraction needle to extend into each trough of the silo.

2. The HE staining apparatus according to claim 1, characterized in that: The liquid injection transverse movement module includes a liquid injection transverse movement fixing seat, which is connected to the dyeing mounting frame. The liquid injection transverse movement fixing seat is provided with a lead screw assembly and a liquid injection transverse movement motor. The output end of the liquid injection transverse movement motor is connected to the lead screw assembly. A liquid injection transverse movement slider is slidably provided on the liquid injection transverse movement fixing seat. The liquid injection transverse movement slider is connected to the lead screw assembly. The liquid injection lifting mounting plate is connected to the liquid injection transverse movement slider.

3. The HE staining apparatus according to claim 1, characterized in that: The liquid injection lifting module includes a liquid injection lifting motor, which is connected to the liquid injection lifting mounting plate. The liquid injection lifting mounting plate is provided with a liquid injection lifting driven wheel, and the output end of the liquid injection lifting motor is provided with a liquid injection lifting drive wheel. A liquid injection lifting transmission belt is fitted on the liquid injection lifting drive wheel and the liquid injection lifting driven wheel. A liquid injection lifting slider is provided on the liquid injection lifting transmission belt, and the liquid injection needle is connected to the liquid injection lifting slider.

4. The HE staining apparatus according to claim 3, characterized in that: The liquid injection lifting mounting plate is equipped with a positioning sensor, and the liquid injection lifting slider is equipped with a detection lever, which can be connected to the positioning sensor.

5. The HE staining apparatus according to claim 1, characterized in that: The syringe cleaning assembly includes a cleaning mounting frame, which is connected to the liquid injection lifting mounting plate. The cleaning mounting frame is provided with a cleaning tank, and the cleaning tank is provided with a needle hole that passes through the upper and lower end faces of the cleaning tank. The liquid injection needle can pass through the needle hole and is in clearance fit with the needle hole. The cleaning tank is provided with an inlet connector and an outlet connector on its side wall. The inlet connector and the outlet connector are respectively connected to the needle hole. The inlet connector is connected to the cleaning liquid, and the outlet connector is connected to the negative pressure suction device.

6. The HE staining apparatus according to claim 1, characterized in that: The lower end face of the injection needle is a sealed structure, and the outlet hole of the injection needle is located on the side wall of the injection needle.

7. The HE staining apparatus according to any one of claims 1-6, characterized in that: The recycling lifting module includes a recycling lifting mounting plate, on which a recycling lifting motor and a rotatable recycling lifting driven wheel are provided. A recycling lifting drive wheel is provided at the output end of the recycling lifting motor. A recycling lifting transmission belt is fitted on the recycling lifting drive wheel and the recycling lifting driven wheel. A recycling lifting slider is provided on the recycling lifting transmission belt. The liquid extraction needle is connected to the recycling lifting slider.

8. The HE staining apparatus according to claim 7, characterized in that: The recovery lifting slider is equipped with a avoidance rotary motor, and the output end of the avoidance rotary motor is equipped with a rotary mounting block. The avoidance rotary motor can drive the rotary mounting block to rotate, and the liquid suction needle is connected to the rotary mounting block.

9. The HE staining apparatus according to claim 8, characterized in that: The recovery lifting slider is equipped with a first sensor, and the rotating mounting block is equipped with a first sensing lever, which can be connected to the first sensor.

10. The HE staining apparatus according to any one of claims 1-6 or any one of claims 8-9, characterized in that: There are two liquid extraction needles, and the distance between the two liquid extraction needles is less than the length of the material tank.

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