A fluorescent agent detection device in cells

Abstract

This invention discloses a fluorescent agent detection device in cells, comprising a frame, a rotating shaft rotatably connected to the top of the frame via a bearing, a mounting base fixedly connected to the outer side of the rotating shaft, multiple excitation light sources fixedly connected to the top of the mounting base, a motor fixedly connected to the bottom of the frame, one end of the motor's output shaft passing through the frame and connected to the rotating shaft via a coupling, and a fixing frame fixedly connected to the top of the frame, with one end of the rotating shaft passing through the fixing frame. This invention not only enables simultaneous or sequential analysis of multiple different targets in the same sample, greatly improving the applicability and detection efficiency of the equipment and reducing detection costs, but also avoids inaccurate detection data due to sample position deviations, improving detection stability. Furthermore, it allows for the simultaneous placement of multiple samples for detection, increasing detection throughput.

Description

Technical Field

[0001] This utility model relates to the field of detection device technology, and in particular to a fluorescent agent detection device in cells. Background Technology

[0002] Fluorescence detection technology is a key analytical tool in fields such as cell biology, medical diagnostics, and drug development. Its basic principle is to use excitation light of a specific wavelength to irradiate a cell sample labeled with a fluorescent dye. When the fluorescent agent in the sample is excited, it emits fluorescence of a longer wavelength. By capturing and analyzing this fluorescence signal, qualitative and quantitative analysis of specific components or states of the cells can be achieved.

[0003] There are some fluorescence detection devices on the market. These devices usually use a fixed single excitation light source and a single detection filter to form a "one-to-one" dedicated optical architecture. However, when users need to perform multi-index analysis on the same sample, the detection process must be paused and the equipment or optical components must be manually replaced, resulting in reduced work efficiency. In order to better address the above problems, promote the development of industry technology, and improve core competitiveness, this application proposes a new composition structure that is different from the existing technology. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a fluorescent agent detection device in cells.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: A fluorescent agent detection device in cells includes a frame, a rotating shaft rotatably connected to the top of the frame via a bearing, a mounting base fixedly connected to the outside of the rotating shaft, multiple excitation light sources fixedly connected to the top of the mounting base, a motor fixedly connected to the bottom of the frame, one end of the motor's output shaft passing through the frame and connected to the rotating shaft via a coupling, a fixing frame fixedly connected to the top of the frame, one end of the rotating shaft passing through the fixing frame, a turntable fixedly connected to the outside of the rotating shaft at the top of the fixing frame, multiple circular grooves formed on one side of the turntable, each of the multiple circular grooves containing a filter, and a detection device for detecting fluorescence located at the top of the fixing frame.

[0006] As a further embodiment of this utility model, the detection device includes a photoelectric sensor, a support frame is fixedly connected to the top of the fixed frame, a housing is fixedly connected to the top of the support frame, a through hole is opened at the bottom of the housing, a fixed cylinder is fixedly connected to the bottom of the housing below the through hole, a lens is provided at the bottom of the fixed cylinder, a mounting bracket is fixedly connected to the top of the housing above the through hole, the photoelectric sensor is fixedly connected to the mounting bracket, and a carrier device for placing cells is provided at the top of the fixed frame.

[0007] As a further embodiment of this utility model, the supporting device includes a slide holder, the top of the fixing frame is provided with a through groove, one side of the through groove is provided with a light-transmitting hole, the slide holder is slidably connected in the through groove, the top of the slide holder is provided with multiple sample slides, and one side of the fixing frame is provided with a driving device for moving the slide holder.

[0008] As a further embodiment of this utility model, the driving device includes a threaded rod, a sliding groove is provided on one side of the fixing frame, the threaded rod is rotatably connected to the sliding groove through a bearing, a threaded block is threadedly connected to the outer side of the threaded rod, and the threaded block slides in the sliding groove, the threaded block is fixedly connected to one side of the plate carrier.

[0009] As a further embodiment of this utility model, the top of the housing is provided with a display screen and a control button group, and the photoelectric sensor is electrically connected to the display screen and the control button group.

[0010] As a further improvement of this utility model, the top of the fixing frame is provided with a first pointer, and the top of the slide holder is provided with a plurality of second pointers.

[0011] As a further embodiment of this utility model, a locking nut is threaded to the outer side of the threaded rod, and a knob is fixedly connected to one end of the threaded rod through the fixing frame.

[0012] The beneficial effects of this utility model are as follows: 1. This utility model is equipped with multiple excitation light sources and a rotatable turntable. The turntable is equipped with multiple filters of different specifications. The turntable is driven by a motor to rotate the shaft, which can flexibly switch between different excitation light sources and filters, thereby realizing the synchronous or sequential analysis of multiple different targets in the same sample, which greatly improves the applicability and detection efficiency of the equipment.

[0013] 2. This utility model is equipped with a first pointer, which, in conjunction with a second pointer, allows the operator to accurately determine whether the cell sample is directly above the light-transmitting hole by observing the relative positions of the first and second pointers. This avoids inaccurate detection data due to sample position deviation and improves the stability of the detection.

[0014] 3. This utility model is equipped with a support device that can be slidably connected to the through groove of the fixed frame through the slide holder, and is equipped with multiple sample slides, which can simultaneously place multiple samples for testing, thereby improving the detection throughput. Attached Figure Description

[0015] Figure 1 This is a three-dimensional structural diagram of a fluorescent agent detection device in cells proposed in this utility model; Figure 2This is a side view of a fluorescent agent detection device in a cell proposed in this utility model; Figure 3 This is a schematic cross-sectional view of the housing structure of a fluorescent agent detection device in cells proposed in this utility model; Figure 4 This is a partial exploded view of a fluorescent agent detection device in a cell proposed in this utility model; Figure 5 This is a partial cross-sectional view of a fluorescent agent detection device in a cell proposed in this utility model; In the diagram: 1. Frame; 2. Fixing frame; 3. Housing; 4. Display screen; 5. Control button group; 6. Support frame; 7. Photoelectric sensor; 8. Mounting bracket; 9. Fixing cylinder; 10. Lens; 11. Mounting base; 12. Rotating shaft; 13. Slide holder; 14. Sample slide; 15. Turntable; 16. Filter; 17. First pointer; 18. Excitation light source; 19. Motor; 20. Threaded rod; 21. Slide groove; 22. Second pointer. Detailed Implementation

[0016] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. The described embodiments are only some embodiments of the present utility model, not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0017] Reference Figures 1-5 A fluorescent agent detection device for cells includes a frame 1. A rotating shaft 12 is rotatably connected to the top of the frame 1 via a bearing. A mounting base 11 is bolted to the outer side of the rotating shaft 12. Multiple excitation light sources 18 are bolted to the top of the mounting base 11. A motor 19 is bolted to the bottom of the frame 1. One end of the output shaft of the motor 19 passes through the frame 1 and is connected to the rotating shaft 12 via a coupling. A fixing frame 2 is bolted to the top of the frame 1. One end of the rotating shaft 12 passes through the fixing frame 2. A rotating shaft 12 is bolted to the outer side of the rotating shaft 12 located on top of the fixing frame 2. The turntable 15 has circular slots on one side that correspond one-to-one with the excitation light source 18. Each of the circular slots contains a filter 16. In use, the motor 19 is a stepper motor, model 57HS09. When the motor 19 is started, the output shaft of the motor 19 rotates, driving the rotating shaft 12 to rotate. The rotation of the rotating shaft 12 causes the turntable 15 mounted on its outer side to rotate accordingly. The filters 16 of different specifications on the turntable 15 rotate sequentially to the positions corresponding to the excitation light source 18. By switching different combinations of excitation light source 18 and filter 16, multiple different targets in the same sample can be excited.

[0018] The top of the mounting bracket 2 is equipped with a detection device for detecting fluorescence, which includes a photoelectric sensor 7. The top of the mounting bracket 2 is fixed with a support frame 6 by bolts, and the top of the support frame 6 is fixed with a housing 3 by bolts. The bottom of the housing 3 has a through hole, and the bottom of the housing 3 is below the through hole and is fixed with a fixing cylinder 9 by bolts. The bottom of the fixing cylinder 9 is equipped with a lens 10, and the top of the housing 3 is above the through hole and is fixed with a mounting bracket 8 by bolts. The photoelectric sensor 7 is fixed to the mounting bracket 8 by bolts. In use, the photoelectric sensor 7 is model E3F-DS30C4. The cell sample to be detected is placed in a suitable position, and the light emitted by the excitation light source 18 is focused by the lens 10 and passes through the through hole to irradiate the cell sample. The fluorescent agent in the cell sample is excited and produces fluorescence. The fluorescence is filtered by the filter 16 and received by the photoelectric sensor 7. The photoelectric sensor 7 converts the received light signal into an electrical signal and transmits it to the subsequent data processing system. By analyzing and processing the electrical signal, relevant information about the fluorescent agent in the cell is obtained.

[0019] In this invention, the top of the mounting bracket 2 is provided with a support device for placing cells. The support device includes a slide holder 13. A through groove is opened on the top of the mounting bracket 2, and a light-transmitting hole is opened on one side of the through groove. The slide holder 13 is slidably connected in the through groove. Multiple sample slides 14 are provided on the top of the slide holder 13. In use, the cell sample to be tested is placed on the sample slide 14, and the slide holder 13 is driven so that the cell sample on the sample slide 14 is above the light-transmitting hole. Multiple samples can be placed for testing at the same time, which improves the detection throughput.

[0020] A drive device for moving the slide holder 13 is provided on one side of the fixed frame 2. The drive device includes a threaded rod 20. A slide groove 21 is provided on one side of the fixed frame 2. The threaded rod 20 is rotatably connected to the slide groove 21 through a bearing. A threaded block is threadedly connected to the outer side of the threaded rod 20 and slides in the slide groove 21. The threaded block is fixed to one side of the slide holder 13 by bolts. When in use, the threaded rod 20 is rotated. Since the threaded rod 20 is threadedly connected to the threaded block, the threaded block will move linearly along the direction of the threaded rod 20, thereby driving the slide holder 13 to slide in the through groove. This allows the cell samples on the sample slide 14 to move above the light-transmitting hole, which facilitates the detection of cell samples at different positions and improves the flexibility and convenience of the detection.

[0021] In particular, the top of the housing 3 is equipped with a display screen 4 and a control button group 5, and the photoelectric sensor 7 is electrically connected to the display screen 4 and the control button group 5. The display screen 4 can display the fluorescence signal intensity and related data detected by the photoelectric sensor 7 in real time, allowing the operator to intuitively understand the detection situation. The operator can set the parameters of the detection device through the control button group 5. The top of the fixing frame 2 is equipped with a first pointer 17, and the top of the slide holder 13 is equipped with multiple second pointers 22. The first pointer 17 and the multiple second pointers 22 are both made of reflective material, which can clearly show their positions under the illumination of the detection environment. The first pointer 17 corresponds to the center position of the light-transmitting hole, and the multiple second pointers 22 correspond to the distribution positions of different cell samples on the sample slide 14. By observing the relative positions of the first pointer 17 and the second pointer 22, the operator can accurately determine whether the cell sample is directly above the light-transmitting hole, avoiding inaccurate detection data due to sample position deviation and improving the stability of the detection. The threaded rod 20 is connected to a locking nut on its outer thread. One end of the threaded rod 20 passes through the fixing frame 2 and is welded to a knob locking nut, which can rotate freely on the threaded rod 20 and be tightened. When the operator rotates the knob, the threaded rod 20 will rotate accordingly, thereby driving the slide holder 13 to move. By adjusting the position of the locking nut, the slide holder 13 can be fixed in the current position, ensuring that the slide holder 13 will not move unexpectedly during the testing process, thereby ensuring the stability of the testing.

[0022] Working principle: When it is necessary to detect fluorescent agents in cell samples, the cell samples are first placed in multiple sample slides 14 on the slide holder 13. Then, the motor 19 is started, and the output shaft of the motor 19 rotates, driving the rotating shaft 12 to rotate. The rotation of the rotating shaft 12 causes the turntable 15 mounted on its outer side to rotate accordingly. Different types of filters 16 on the turntable 15 rotate sequentially to the positions corresponding to the excitation light source 18. By switching different combinations of excitation light sources 18 and filters 16, multiple different targets in the same sample can be excited. The light source 18 emits light through the light-transmitting hole on the fixing frame 2 to illuminate the sample slides 14. The cell samples irradiated by the excitation light source 18 will emit fluorescence of a specific wavelength. After being focused by the lens 10 on the fixing cylinder 9, it is captured by the photoelectric sensor 7 mounted on the mounting bracket 8. The photoelectric sensor 7 detects the captured fluorescence. The light signal is converted into an electrical signal and transmitted to the display screen 4 for display. At the same time, the detection parameters can be set and adjusted through the control button group 5. During the detection process, if it is necessary to move the position of the slide holder 13 to adjust the detection position, the knob at one end of the threaded rod 20 can be rotated. The rotation of the threaded rod 20 drives the threaded block to slide in the slide groove 21. The threaded block drives the slide holder 13 to slide in the through groove of the fixing frame 2, thereby realizing the adjustment of the position of the sample slide 14. The first pointer 17 and the second pointer 22 can assist in the precise alignment of the position. When the slide holder 13 is moved to the appropriate position, the locking nut on the outside of the threaded rod 20 is tightened to fix the slide holder 13 in the current position, and the detection work can continue. Through this working principle, the fluorescent agent detection device in the cell can efficiently and accurately detect and analyze the fluorescent agents in the cell sample.

[0023] Furthermore, although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A fluorescent agent detection device in cells, comprising a frame (1), characterized in that, The top of the frame (1) is rotatably connected to a rotating shaft (12) via a bearing. A mounting base (11) is fixedly connected to the outside of the rotating shaft (12). Multiple excitation light sources (18) are fixedly connected to the top of the mounting base (11). A motor (19) is fixedly connected to the bottom of the frame (1). One end of the output shaft of the motor (19) passes through the frame (1) and is connected to the rotating shaft (12) via a coupling. A fixed frame (2) is fixedly connected to the top of the frame (1). One end of the rotating shaft (12) passes through the fixed frame (2). A turntable (15) is fixedly connected to the outside of the rotating shaft (12) at the top of the fixed frame (2). Multiple circular grooves are opened on one side of the turntable (15). A filter (16) is provided in each of the multiple circular grooves. A detection device for detecting fluorescence is provided at the top of the fixed frame (2).

2. The fluorescent agent detection device in a cell according to claim 1, characterized in that, The detection device includes a photoelectric sensor (7), a support frame (6) is fixedly connected to the top of the fixed frame (2), a housing (3) is fixedly connected to the top of the support frame (6), a through hole is opened at the bottom of the housing (3), a fixed cylinder (9) is fixedly connected to the bottom of the housing (3) below the through hole, a lens (10) is provided at the bottom of the fixed cylinder (9), a mounting bracket (8) is fixedly connected to the top of the housing (3) above the through hole, the photoelectric sensor (7) is fixedly connected to the mounting bracket (8), and a carrier device for placing cells is provided at the top of the fixed frame (2).

3. The fluorescent agent detection device in a cell according to claim 2, characterized in that, The carrier device includes a slide holder (13), the top of the fixing frame (2) is provided with a through groove, one side of the through groove is provided with a light-transmitting hole, the slide holder (13) is slidably connected in the through groove, the top of the slide holder (13) is provided with multiple sample slides (14), and one side of the fixing frame (2) is provided with a driving device for moving the slide holder (13).

4. The fluorescent agent detection device in a cell according to claim 3, characterized in that, The driving device includes a threaded rod (20), and a slide groove (21) is provided on one side of the fixed frame (2). The threaded rod (20) is rotatably connected to the slide groove (21) through a bearing. A threaded block is threadedly connected to the outer side of the threaded rod (20), and the threaded block slides in the slide groove (21). The threaded block is fixedly connected to one side of the plate carrier (13).

5. The fluorescent agent detection device in a cell according to claim 3, characterized in that, The top of the housing (3) is provided with a display screen (4) and a control button group (5), and the photoelectric sensor (7) is electrically connected to the display screen (4) and the control button group (5).

6. The fluorescent agent detection device in a cell according to claim 4, characterized in that, The top of the fixture (2) is provided with a first pointer (17), and the top of the slide holder (13) is provided with a plurality of second pointers (22).

7. The fluorescent agent detection device in a cell according to claim 4, characterized in that, The threaded rod (20) is threaded with a locking nut on its outer side, and one end of the threaded rod (20) is fixedly connected to a knob through the fixing frame (2).

Images