A test card for detecting the content of icariin in traditional Chinese medicinal materials

Abstract

This utility model relates to the field of detection card technology and discloses a detection card for detecting the content of icariin in traditional Chinese medicine, including a lower shell and an upper shell: the lower shell and the upper shell are connected by an insertion joint, and a PVC sheet is connected to the bottom of the lower shell; the upper shell has a sample drop hole and an observation groove, and an annular splitting spring plate is connected inside the sample drop hole. In this detection card for detecting the content of icariin in traditional Chinese medicine, the sample receiving ball at the bottom of the sample drop hole can accommodate the dropped sample, and the groove on the outside of the sample receiving ball allows the sample inside the ball to flow slowly, preventing excessive sample accumulation in one place on the sample pad. This ensures that the sample is received and processed by the sample pad at a constant speed, guaranteeing the cleanliness of the processed sample. The design of the sample pad and processing pad also facilitates full contact between the sample and the gold-labeled pad, further improving the accuracy of sample detection.

Description

Technical Field

[0001] This utility model relates to the field of detection card technology, specifically a detection card for detecting the content of icariin in traditional Chinese medicine. Background Technology

[0002] Epimedoside is the main active ingredient of Epimedium, an 8-isopentenyl flavonoid glycoside compound. It can be extracted from the dried stems and leaves of Epimedium sagittatum, Epimedium pubescens, Epimedium wushanense, and Epimedium koreanum. It is a pale yellow needle-like crystal, soluble in ethanol and ethyl acetate, but insoluble in ether, benzene, and chloroform. The aerial parts of Epimedium mainly contain flavonoids, while the underground parts mainly contain flavonoids and alkaloids. In addition, Epimedium plants also contain hundreds of chemical components such as lignans, anthraquinones, anthocyanins, sesquiterpenes, phenylethanol glycosides, polysaccharides, glucose, fructose, phytosterols, palmitic acid, stearic acid, linolenic acid, and potassium chloride. These components are distributed in different Epimedium plants. Epimedoside can increase cardiovascular blood flow, promote hematopoietic function, immune function, and bone metabolism, and also has the effects of tonifying the kidneys and strengthening yang, and anti-aging.

[0003] Current detection strips typically use colloidal gold-labeled antibodies and consist of a base plate, an NC membrane, a sample pad, a conjugate pad, absorbent paper, and a casing. The sample pad delivers the sample to other parts of the strip and serves as a pretreatment function. The conjugate pad contains the label, which affects label release and detection sensitivity. The NC membrane is the main driving force for the chromatographic reaction and is also the site of the immune response and signal detection. The absorbent pad is located at the end of the strip and helps maintain the flow rate of the liquid on the membrane, preventing sample backflow.

[0004] However, the existing test cards have certain defects. Their sample drop hole and observation slot are completely exposed, and external contaminants can easily cause surface contamination, affecting their detection accuracy. At the same time, if the sample pad in the test card is not processed in time during the detection process, some sample solutions will have poor binding with the gold label pad, affecting the subsequent detection accuracy.

[0005] Therefore, it is necessary to propose a detection card for detecting the content of icariin in traditional Chinese medicinal materials. Utility Model Content

[0006] To address the shortcomings of existing technologies, this invention provides a detection card for detecting the content of icariin in traditional Chinese medicine materials. It has the advantages of improving the detection accuracy of samples and reducing the contamination of the detection card by external pollutants, thus solving the problems mentioned in the background art.

[0007] This utility model provides the following technical solution: a detection card for detecting the content of icariin in traditional Chinese medicinal materials, comprising a lower shell and an upper shell:

[0008] The lower housing and the upper housing are connected by an insertion joint, and a PVC sheet is connected to the bottom of the interior of the lower housing.

[0009] The upper shell is provided with a sample drop hole and an observation groove. The inside of the sample drop hole is connected to an annular splitting spring plate. The bottom of the annular splitting spring plate is fixedly connected to a sample receiving ball. Several sets of slots are provided on the outside of the sample receiving ball. The inside of the observation groove is connected to a transparent plate.

[0010] Preferably, a sample pad is provided on one side of the upper surface of the PVC sheet, a treatment pad is attached to the other side of the sample pad, a gold labeling pad is attached to the other side of the treatment pad, an absorption pad is provided on the other side of the upper surface of the PVC sheet, an NC film is attached to the opposite sides of the absorption pad and the gold labeling pad, a detection line T and a control line C are respectively provided on the NC film, and the gold labeling pad is coated with colloidal gold particles adsorbed with anti-icariin monoclonal antibody or latex microspheres or fluorescent microspheres chemically bonded with anti-icariin monoclonal antibody.

[0011] Preferably, a sample drop hole is provided above the upper surface of the sample pad, and an observation groove is provided above the upper surface of the NC membrane.

[0012] Preferably, the detection line T is coated with icariin antigen, and the control line C is coated with goat anti-mouse secondary antibody.

[0013] Preferably, the detection line T is located on the side closer to the gold label pad, and the quality control line C is located on the side closer to the absorbent pad.

[0014] Preferably, the lower shell and the upper shell cooperate to form a hollow cuboid, and the lengths of the lower shell and the upper shell are the same.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] This detection card for determining the icariin content in traditional Chinese medicine utilizes a ring-shaped splitting spring plate inside the sample dropper hole to effectively prevent external impurities from entering the card. The ring-shaped splitting spring plate is designed with an elastic structure, allowing liquid to be delivered to the sample pad via downward insertion of the pipette output end. A processing pad is added between the sample pad and the gold label pad for sample purification, ensuring sample purity and facilitating subsequent detection of icariin content. The processing pad and sample pad are made of the same material, making it easy to add more. Additionally, an observation chamber is provided with… The transparent plate effectively prevents external infectious agents from entering the NC membrane, thus avoiding contamination and ensuring detection accuracy. The sample receiving ball at the bottom of the sample dropper hole can hold the dropped sample, and the groove on the outside of the sample receiving ball allows for slow flow of the sample inside the ball, preventing excessive sample accumulation in one place on the sample pad. This ensures that the sample is received and processed by the sample pad at a constant rate, guaranteeing the cleanliness of the processed sample. The combination of the sample pad and the processing pad facilitates full bonding between the sample and the gold-labeled pad, further improving the accuracy of sample detection. Attached Figure Description

[0017] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0019] Figure 2 This is a schematic diagram of the separate upper and lower shell structures of this utility model;

[0020] Figure 3 This utility model Figure 1 Cross-sectional structural diagram.

[0021] The attached diagram lists the components represented by each number as follows:

[0022] 1. Lower shell; 110. Sample pad; 120. Processing pad; 130. Gold label pad; 140. NC membrane; 150. Detection line T; 160. Quality control line C; 170. Absorption pad; 180. PVC sheet;

[0023] 2. Upper shell;

[0024] 3. Sample drop inlet hole; 310. Annular splitting spring plate; 320. Sample receiving ball; 330. Groove;

[0025] 4. Observation slot; 410. Transparent plate. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0027] Please see Figure 1 , Figure 2 and Figure 3 A test card for detecting the content of icariin in traditional Chinese medicine includes a lower shell 1 and an upper shell 2.

[0028] The lower housing 1 and the upper housing 2 are connected by an insertion joint, and a PVC sheet 180 is connected to the bottom of the interior of the lower housing 1.

[0029] The upper shell 2 is provided with a sample drop hole 3 and an observation groove 4. The inside of the sample drop hole 3 is connected to an annular splitting spring plate 310. The bottom of the annular splitting spring plate 310 is fixedly connected to a sample receiving ball 320. Several sets of slots 330 are provided on the outside of the sample receiving ball 320. The inside of the observation groove 4 is connected to a transparent plate 410.

[0030] When the sample is dispensed, the output end of the pipette is inserted into the sample dispensing hole 3. At this time, the output end of the pipette separates from the annular splitting plate 310, and the sample is slowly injected into the sample receiving ball 320. The sample is then slowly separated from the sample receiving ball 320 through the slot 330 to ensure uniform absorption of the sample. The test results are then observed through the transparent plate 410 on the observation slot 4. The annular splitting plate 310 and the transparent plate 410 also help to prevent external contaminants from entering the test card, thus ensuring the accuracy of the test results.

[0031] As a preferred embodiment of this utility model, a sample pad 110 is provided on one side of the upper surface of the PVC sheet 180, a treatment pad 120 is attached to the other side of the sample pad 110, a gold label pad 130 is attached to the other side of the treatment pad 120, an absorption pad 170 is provided on the other side of the upper surface of the PVC sheet 180, an NC membrane 140 is attached to the opposite sides of the absorption pad 170 and the gold label pad 130, a detection line T150 and a control line C160 are respectively provided on the NC membrane 140, the detection line T150 is coated with icariin antigen, and the control line C160 is coated with goat anti-mouse secondary antibody.

[0032] During sample processing, the sample is pretreated using sample pad 110 and processing pad 120, and the sample pad 110 and processing pad 120 have the same structure and material. The gold labeling pad 130 is sprayed with colloidal gold particles adsorbed with anti-icariin monoclonal antibody or latex microspheres or fluorescent microspheres chemically bonded with anti-icariin monoclonal antibody. The NC membrane 140 has a detection line T150 coated with icariin antigen and a quality control line C160 coated with goat anti-mouse secondary antibody. The principle is that icariin in the sample solution competes with the icariin antigen on the T line to bind to the gold labeling antibody. When the icariin content in the sample solution is high, more gold-labeled antibodies bind, resulting in fewer gold-labeled antibodies binding to the T line, and a lighter T line color. Conversely, when the icariin content in the sample solution is low, fewer gold-labeled antibodies bind, resulting in more gold-labeled antibodies binding to the T line, and a darker T line color. The goat anti-mouse antibody in the C line can bind to all gold-labeled antibodies. Thus, the T and C lines can exhibit different colors depending on the amount of icariin in the sample solution. Qualitative detection cards can determine the icariin content level by directly comparing the colors of the T and C lines; a darker T line often indicates a lower concentration of the target substance in the sample solution. Quantitative detection cards can read the colors of the T and C lines using a reader; different values ​​represent different contents. The icariin content information can be directly obtained using a standard curve.

[0033] As a preferred technical solution of this utility model, a sample drop hole 3 is provided above the upper surface of the sample pad 110, an observation groove 4 is provided above the upper surface of the NC membrane 140, a detection line T150 is provided on the side near the gold label pad 130, and a quality control line C160 is provided on the side near the absorption pad 170.

[0034] As a preferred technical solution of this utility model, the lower shell 1 and the upper shell 2 cooperate to form a hollow cuboid, and the length values ​​of the lower shell 1 and the upper shell 2 are the same.

[0035] The specific operating procedure is as follows: Place the test card on the constant temperature incubator, use a pipette to draw 120 μL of the sample solution containing icariin, and add it vertically into the sample drop hole 3 of the test card. Cover the incubator and incubate for 10 minutes. After the test card has been incubated, immediately insert it into the liquid addition hole of the reader, click "Start Test", and read the result.

[0036] In the description of this utility model, it should be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "side", "top", "inner", "front", "center", "both ends", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0037] In this utility model, unless otherwise explicitly specified and limited, the terms "installation", "setting", "connection", "fixing", "screw connection", etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal connection of two components or the interaction between two components. Unless otherwise explicitly limited, those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0038] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A test card for detecting the content of icariin in traditional Chinese medicinal materials, comprising a lower shell (1) and an upper shell (2), characterized in that: The lower housing (1) and the upper housing (2) are connected by an insertion, and a PVC sheet (180) is connected to the bottom of the interior of the lower housing (1); The upper shell (2) is provided with a sample drop hole (3) and an observation groove (4). The sample drop hole (3) is connected to an annular splitting spring plate (310). The bottom of the annular splitting spring plate (310) is fixedly connected to a sample receiving ball (320). Several sets of slots (330) are provided on the outside of the sample receiving ball (320). The observation groove (4) is connected to a transparent plate (410).

2. The detection card for detecting the content of icariin in traditional Chinese medicinal materials according to claim 1, characterized in that: A sample pad (110) is provided on one side of the upper surface of the PVC sheet (180), a treatment pad (120) is attached to the other side of the sample pad (110), a gold labeling pad (130) is attached to the other side of the treatment pad (120), an absorption pad (170) is provided on the other side of the upper surface of the PVC sheet (180), an NC membrane (140) is attached to the opposite sides of the absorption pad (170) and the gold labeling pad (130), a detection line T (150) and a quality control line C (160) are respectively provided on the NC membrane (140), and the gold labeling pad (130) is sprayed with colloidal gold particles adsorbed with anti-icariin monoclonal antibody or latex microspheres or fluorescent microspheres chemically bonded with anti-icariin monoclonal antibody.

3. The detection card for detecting the content of icariin in traditional Chinese medicinal materials according to claim 2, characterized in that: A sample drop hole (3) is provided above the upper surface of the sample pad (110), and an observation groove (4) is provided above the upper surface of the NC membrane (140).

4. The detection card for detecting the content of icariin in traditional Chinese medicinal materials according to claim 2, characterized in that: The detection line T(150) is coated with icariin antigen, and the control line C(160) is coated with goat anti-mouse secondary antibody.

5. A detection card for detecting the content of icariin in traditional Chinese medicinal materials according to claim 2, characterized in that: The detection line T (150) is located on the side near the gold label pad (130), and the quality control line C (160) is located on the side near the absorbent pad (170).

6. The detection card for detecting the content of icariin in traditional Chinese medicinal materials according to claim 1, characterized in that: The lower shell (1) and the upper shell (2) cooperate to form a hollow cuboid, and the lengths of the lower shell (1) and the upper shell (2) are the same.

Images