NEW BIOCIDAL PRODUCTS

Description

[0001] The present invention relates to a new generation of biocidal products which do not present chronic risks and very few acute impacts on health and the environment.

[0002] Indeed, the active substances and adjuvants that make up the biocidal formulations according to the invention are of plant origin, preferably agricultural, and renewable, and are also totally, naturally and rapidly biodegradable.

[0003] The various applications of these new biocidal products are also claimed.

[0004] Biocidal products are commonly used in a wide variety of products, including household and / or industrial disinfectants, insecticides, wood treatment products, etc.

[0005] These products can be used to clean and disinfect all types of surfaces, including skin.

[0006] Biocidal products are intended to destroy, repel or render ineffective undesirable organisms, whether considered harmful or not, such as bacteria, viruses, fungi, to prevent their action or to combat them in any other way by chemical or biological action.

[0007] These product categories are chemically or biologically active products, and, as such, are likely to have harmful effects on humans, animals and / or the environment.

[0008] More specifically, a biocide is generally characterized by its field of application: bactericidal, fungicidal, antiviral and / or insecticidal.

[0009] Bactericides are effective against certain Gram-positive and / or Gram-negative bacteria. Fungicides are effective against certain fungi and yeasts. Antivirals are effective against certain viruses.

[0010] Biocidal agents can therefore pose a major risk to human and animal health, as well as to the environment; indeed, etymologically, "bios" means "life" and "-cide" means "that which kills." In fact, biocidal agents can act for a long time, diffuse, be partially degraded, or not degrade at all depending on their nature, and they are then considered (bio)accumulative and toxic.

[0011] The active substances that make up biocides are generally active ingredients of petrochemical origin.

[0012] A few rare plant-based substances can be listed, such as ethanol or essential oils with limited bacteriostatic properties. Furthermore, these substances are not available on professional markets.

[0013] Finally, biocides may also contain a mixture of the last two categories of components, i.e. active ingredients of petrochemical origin and substances of plant origin, in order to achieve the desired effectiveness.

[0014] We know, in the state of the art, the quaternary ammonium derivatives which represent a major class of chemically derived biocidal substances which are used in surface disinfectants and for hand hygiene.

[0015] Other substances are also used such as sodium hypochlorite (bleach), di- or tri-amines or 5-chloro-2-(2,4 dichlorphenoxy)phenol (Triclosan ®< ).

[0016] Their regulatory classification with regard to their concerning, corrosive, sensitizing and highly toxic effects on aquatic organisms, as well as the suspicion of CMR (Carcinogenic, Mutagenic, Reprotoxic) and endocrine-disrupting effects of some representatives of this class, requires the greatest caution in the use of these products.

[0017] International awareness on this subject has led to the adoption of European Regulations (REACH: 1907 / 2006 and Biocides: 528 / 2012) aimed at limiting the marketing of dangerous chemical substances and strictly regulating their use.

[0018] Indeed, the market for biocidal products for Europe represents a volume of 300,000 to 750,000 tonnes per year (Study of the impact of the Biocidal Products Directive 98 / 8 / EC - 2007) which corresponds to an annual global market of 7 billion euros.

[0019] There is therefore considerable interest in developing new biocidal products to combat undesirable microorganisms. These biocides must be able to be completely degraded naturally after use, so as to reduce risks to humans, animals, and the environment, while limiting the development of resistance.

[0020] In particular, for humans and animals, these risks can be "direct", related to so-called acute toxicity, and especially "indirect", related to chronic toxicity.

[0021] Furthermore, biocidal products must be effective at low concentrations in order to offer industrial and economic solutions that comply with the economic positioning of health markets.

[0022] In order to offer biocidal products that are not toxic and / or harmful to humans and the environment, and that are also biodegradable and biocompatible for humans, biocides of natural origin have been developed.

[0023] Indeed, plant-based biocidal products appear to be the most appropriate to meet these combined objectives.

[0024] Most plant-based biocidal solutions contain a cleansing base combined with a disinfectant base. However, these products do not offer the same biocidal efficacy or ease of use (dose / action time / spectrum of action ratio) as biocidal solutions containing chemically synthesized active ingredients and / or adjuvants.

[0025] Therefore, there is a need to develop a sustainable and safe alternative to the use of chemical-synthetic products, which provides biocidal efficacy and ease of use in a product that can be diluted.

[0026] We already know, from patent document WO 2012 / 114039, of a biocidal composition comprising: between 0.01% and 20% chelating agent; between 0.03% and 25% non-ionic surfactants of the polyol type and preferably between 0.03% and 25% non-ionic surfactants of the glycoside, polyglycerol ester or sorbitan ester type; between 0.03% and 25% anionic surfactants, preferably of the carboxylate or polycarboxylate type; between 0.1% and 75% of one or more organic acids; between 0.001% and 8% natural perfume.

[0027] However, this composition, although of plant origin and having biocidal activity on certain pathogens, can still be improved.

[0028] Indeed, the composition described in this document does not present optimal effectiveness against undesirable and potentially pathogenic organisms, for example certain strains of fungi or certain undesirable or pathogenic viruses.

[0029] The present invention thus relates to a new generation of biocidal formulations for the cleaning and disinfection of all types of surfaces.

[0030] Their impact on the environment is very limited, if not nonexistent, and in any case, it is not considered a concern. This is due to the toxicological data for the components and the mixture, which are significantly below the required classification thresholds.

[0031] Indeed, these biocidal formulations are derived from renewable plant resources, preferably agricultural resources, and they are fully biodegradable throughout the formulation.

[0032] The active substance of the biocidal formulations according to the invention consists of at least one organic acid of plant origin. This organic acid may be derived directly from a plant resource, preferably agricultural, or from a processed agricultural resource.

[0033] The present invention describes an aqueous liquid composition with a virucidal effect against strains of viruses belonging to the adenovirus family, or with a virucidal effect against rotavirus, or with a fungicidal effect against fungi belonging to the species Aspergillus niger, said composition comprising a concentrated biodegradable biocidal formulation and conforming to the subject matter of independent claim 1.

[0034] Advantageously, the proportion of organic acid in the biocidal formulation conforms to the subject matter of dependent claim 4. As for the proportion of anionic surfactant, this conforms to the subject matter of dependent claim 4, preferably conforms to the subject matter of dependent claim 5.

[0035] The biocidal formulation used to prepare the diluted composition according to the invention comprises compounds derived from plant resources, particularly agricultural resources, which are entirely renewable. Consequently, said biocidal formulation is fully biodegradable.

[0036] More specifically, the composition is 100% biodegradable according to the results of tests relating to the OECD experimental protocol.

[0037] In addition to a significantly reduced environmental impact compared to the state of the art, the biocidal formulations used for the preparation of the diluted composition according to the invention present an innovative effect of making the biocidal active biologically available thanks to adapted surfactant structures and under the effect of physico-chemical modifications of the mixture, which results in a desired combination between the constituents and performance allowing the substitution, at equivalent concentration, of chemical and hazardous products on the market.

[0038] This biocidal formulation exhibits effective action against certain undesirable or pathogenic organisms, and an improved effect compared to previously known biocidal formulations. The action of the formulation is illustrated in the examples below.

[0039] In particular, this biocidal formulation makes it possible to combat fungi belonging to the species Aspergillus niger, which is likely to be toxic and pathogenic, notably by causing pulmonary mycoses in humans.

[0040] The composition according to the invention also exhibits biocidal activity, particularly virucidal activity, against strains of viruses belonging to the adenovirus family, which can cause illnesses such as pharyngitis, pneumonia, and conjunctivitis. Virucidal activity has also been demonstrated against rotaviruses, which are responsible for gastroenteritis, among other things.

[0041] With regard to the organic acids that can be used in the composition of biocidal formulations according to the present invention, citric acid, lactic acid, succinic acid, pyruvic acid, and glycolic acid may be mentioned in particular.

[0042] The choice of organic acid depends on the application area of ​​the biocidal formulations and the target organisms to be eliminated or whose development is to be slowed. In one particular embodiment, the organic acid(s) are lactic acid and citric acid.

[0043] In an even more preferred embodiment, only lactic acid is used.

[0044] Indeed, lactic acid is one of the essential elements of human metabolism, as well as the metabolism of animals and microorganisms. Lactic acid intrinsically possesses properties that interfere with microorganisms because it acts directly on their pH and their energy cycle. Furthermore, it also has an antimicrobial effect by inhibiting their growth (for Escherichia coli among others).

[0045] In addition to the organic acid, the concentrated biocidal formulation according to the invention also comprises at least one anionic surfactant.

[0046] This one is advantageously chosen from among sodium carboxylates comprising a number of carbon atoms between 5 and 16.

[0047] The anionic surfactant may also consist of a potassium carboxylate comprising a number of carbon atoms between 5 and 16.

[0048] Preferably, the carbon chain of anionic surfactants is derived from fatty alcohols or vegetable fatty acids.

[0049] Thus, according to the invention, the anionic surfactant consists of an alkyl carboxylate of the sodium caprate or sodium caprylate type.

[0050] In an example not covered by the claims, the anionic surfactant may be sodium laurate, C8-C16 sodium, ammonium or potassium alkyl sulfate, or a carboxylated fatty acid ester.

[0051] The anionic surfactant can also be made up of acidic species derived from these salts.

[0052] The concentrated biocidal formulation used for the preparation of the diluted composition according to the invention may also include other ingredients.

[0053] Thus, the said formulation may include, in addition to the organic acid and the anionic surfactant, at least one non-ionic surfactant and / or at least one natural fragrance and / or at least one chelating agent.

[0054] Non-ionic surfactants or other components such as essential oils or chelating agents that may be included in the composition of the biocidal product according to the invention are selected based on the type of performance and application required: a judicious mixture is necessary so as not to disrupt or alter the initially desired biocidal properties. Indeed, for example, effective disinfectant and / or degreasing properties are necessary in the food service or food processing industries, with products that are low or non-toxic, in order to avoid any concerning chemical contamination, particularly the presence of chemical residues on surfaces after the disinfection procedure.

[0055] In a particular embodiment, the biocidal formulation used for the preparation of the diluted composition according to the invention may comprise at least one non-ionic surfactant, preferably in a proportion of between 2 and 15% by mass relative to the total mass of the formulation.

[0056] Advantageously, this non-ionic surfactant is chosen from glycosides and alkyl polyglycosides whose carbon chain comprises between 5 and 20 carbon atoms, more advantageously between 5 and 18 carbon atoms, and the hydrophilic group is preferentially composed of pentose or hexose residues.

[0057] Thus, preferably, the formulation used for the preparation of the diluted composition according to the invention may include a non-ionic surfactant selected from octyl glucosides, decyl glucosides, lauryl glucosides, coco glucosides, D-glucopyranose, D-xylopyranose C10-C16 alkylpolyglycosides.

[0058] The chelating agents that can be included in the composition of the biocidal formulation according to the invention are conventional agents in the field of cleaning and disinfecting surfaces for domestic or professional use.

[0059] They are selected from among the chelating agents commonly used in biocidal products, such as sorbic acid, from rowan, or oxalic acid, from the roots or rhizomes of many plants (sorrel, beetroot, etc.), sequestering monomers or polymers such as chicory extracts, or amino acid derivatives.

[0060] Furthermore, the chelating agent may also, in an embodiment not covered by the text of the claims, be citric acid, derived from lemon juice or from the fermentation of simple or complex plant sugars, in particular glucose, fructose, and sucrose, or derived from the fermentation of agricultural material. This basic agricultural material may consist, for example, of molasses residues from which gluconate-type complexing agents can be obtained.

[0061] Preferably, the chelating agent(s) are present at a rate of 0.1% to 10% by mass relative to the total mass of the formulation.

[0062] The olfactory properties of the biocidal products according to the invention can be conferred by the presence of at least one natural perfume derived from a renewable and biodegradable natural resource.

[0063] This natural fragrance can be advantageously chosen from among essential oils, plant essences or even plant extracts.

[0064] Examples include mint A, mint N or eucalyptus G.

[0065] Preferably, the natural perfume(s) are present in the concentrated biocide formulation at a rate of 0.01% to 10% by mass relative to the total mass of the formulation.

[0066] The presence of the fragrance will provide an advantageous olfactory note without contributing to the product's biocidal activity. Therefore, it can be withdrawn in situations where fragrance-free formulations are required, such as in the food industry.

[0067] The formulations comply with European regulations in terms of effectiveness. They meet the standards EN1040, EN1276, EN1656, EN 1275, EN 13697, EN1650 and EN14476+A1 under dirty or clean conditions.

[0068] Furthermore, these effective formulations are safe for humans and harmless to the environment.

[0069] The biocidal formulations used to prepare the diluted composition according to the invention are suitable for use at dilution rates and for action times comparable to those required by the professional market. Furthermore, they meet the required biocidal efficacy standards, pose no particular hazards during application, are biodegradable, and have no ecotoxicity after disposal.

[0070] Thus, an aqueous liquid composition may comprise at least the biocidal formulation described above and water, and all dilutions in water of said composition up to a minimum concentration of 0.2% of said composition. Such an example is not covered by the text of the claims but is considered useful for understanding the invention.

[0071] The remainder of the composition comprises at least water. Thus, in a particular embodiment, the aqueous liquid composition according to the invention may also comprise other ingredients so as to improve the cleaning and / or organoleptic properties, without however altering the biocidal activity of said formulation.

[0072] In an example of an embodiment not covered by the text of the claims, the biocidal formulation according to the invention is present, in said aqueous liquid composition, in a proportion of between 0.2 and 2% by mass, relative to the total mass of said composition.

[0073] In other words, the said formulation can be advantageously diluted between 50 and 500 times and still be active on the undesirable organisms that one wishes to eliminate.

[0074] The biocidal formulation according to the invention is present, in said aqueous liquid composition, in a proportion of between 1 and 2% by mass, more advantageously between 1.5 and 2% by mass, relative to the total mass of said composition.

[0075] The following examples illustrate this effectiveness, particularly against certain pathogenic or undesirable strains of yeasts, fungi and bacteria, but also against certain pathogenic viruses.

[0076] In particular, biocidal formulations are especially interesting because they remain effective even at high dilution rates, and have comparable effectiveness to more concentrated formulations which are less economically attractive.

[0077] The applications envisaged for the biocidal formulations according to the invention are based on a disinfectant action but also detergent, degreasing, emollient, olfactory, etc. action.

[0078] Examples include, but are not limited to, applications of the formulations according to the invention to anti-foam products for cleaning masonry structures; products for cleaning pontoons and boat hulls of the moss and algae that colonize them; disinfectant detergent solutions for all types of surfaces; solutions for hand hygiene by washing or rubbing; solutions for disinfecting foodstuffs; solutions for disinfecting premises, buildings and transport and livestock equipment; surface disinfection solutions for the food, cosmetic or pharmaceutical industries; disinfection solutions for premises and / or transport of household waste; and disinfection solutions for milking equipment, teats, and animal bedding.

[0079] In a particular embodiment, the biocidal formulations used for the preparation of the diluted composition according to the invention are used for hand hygiene, by washing or rubbing, in liquid or semi-liquid form, with or without rinsing with water.

[0080] The present description also describes a substrate impregnated with a concentrated biocide formulation used for the preparation of the diluted composition according to the invention, allowing the application of said formulation to a surface to be cleaned and disinfected.

[0081] This substrate can consist of a fabric, a wipe or a towel.

[0082] On its substrate, the formulation can be pure and dehydrated and only activated once in the presence of water, or it can be already diluted and ready to use.

[0083] In other words, the substrate can also be impregnated with the aqueous liquid composition as described above, said composition comprising at least the biocide formulation and water, said formulation being present in a proportion of between 0.2% and 2% by mass relative to the total mass of said composition.

[0084] The embodiments mentioned above and relating to a substrate impregnated with a pure formulation or an aqueous liquid composition with the formulation present in a proportion between 0.2 and 2% are not covered by the text of the claims, but are considered useful for understanding the invention. Example of a biocide formulation :

[0085] In one embodiment, the biocidal formulation used for the preparation of the diluted composition according to the invention comprises the following compounds, in the following proportions: 2.4% non-ionic surfactants of the glycoside type; 5% anionic surfactants of the fatty acid salt type with a chain containing 8 to 14 carbon atoms of the sodium cocoate type; 91% lactic acid 1.6% water.

[0086] This formula is classified as an irritant under current regulations, just like the lactic acid used in its formulation. This classification allows it to bypass the lengthy and costly approval processes required for market access. Furthermore, this formulation poses no danger to humans or the environment compared to chemically derived substances currently on the market.

[0087] This formulation is diluted according to the surfaces to be cleaned and sanitized, and the desired level of disinfection effectiveness. The diluted, active formula for surface disinfection presents no known or regulated hazards.

[0088] In a second embodiment, the biocidal formulation used for the preparation of the diluted composition according to the invention is composed as follows: 76% lactic acid; 24% anionic surfactants of the fatty acid salt type with a chain containing 8 to 10 carbon atoms of the sodium alkyl carboxylate type.

[0089] In a third embodiment, the biocidal formulation used for the preparation of the diluted composition according to the invention is composed as follows: 80% lactic acid; 5% anionic surfactants of the type sodium cocoate fatty acid salts; 1% fragrance composition based on plant extracts; 14% water.

[0090] In a fourth embodiment, the biocidal formulation used for the preparation of the diluted composition according to the invention is composed as follows: 76% lactic acid; 10% non-ionic alkyl polyglycoside surfactants; 10% anionic C10-C12 sodium carboxylate surfactants; 4% water. Efficacy tests of the biocide formulation

[0091] The biocidal formulation that was tested is the one specified above and includes: 2.4% non-ionic surfactants of the C10-C16 alkyl polyglycosides type; 5% anionic surfactants of the fatty acid salts type sodium caprylate; 91% lactic acid; 1.6% water. 1. Yeasts - Candida albicans

[0092] This quantitative suspension test for evaluating the basic yeasticidal activity of the formulation according to the invention is carried out by a Microbiology Laboratory. This test is performed under dirty conditions and additional conditions.

[0093] Method :A dilution / neutralization method is applied to a formulation according to the invention. The neutralizer consists of sodium chloride (0.85% w / v), Na₂CO₃ (0.1%), supplemented with distilled water.

[0094] The formulation used for the preparation of the diluted composition according to the invention is diluted in physiological water at the following concentrations: 1%, 1.2%, 1.5% and 2%.

[0095] The tests are performed on a strain of Candida albicans (ATCC 90028) at a temperature of 20°C (± 1°C). The contact time is limited to 5 minutes (± 10 seconds).

[0096] The interfering substance is bovine albumin (fraction V) at a concentration of 3.00 g / l. This constitutes the soiling condition.

[0097] The tests are incubated at a temperature of 25.0°C (± 0.2°C) for 48 hours.

[0098] Results: in accordance with the provisions of standard NF EN 1275 (April 2006), the formula according to the invention exhibits yeasticidal activity under dirty conditions (3.00 g / l bovine albumin) after 5 minutes of contact at 20°C with a reference strain of Candida albicans for the concentration of 1.2%. 2. Mushrooms - Aspergillus niger

[0099] The fungicidal activity of the biocidal formulation used to prepare the diluted composition according to the invention was also tested, particularly on a fungus belonging to the species Aspergillus niger.

[0100] The biocidal formulation is diluted to a concentration of 2% in water.

[0101] The tests were conducted at a temperature of 20°C (± 1°C). Two contact times were tested: 15 min and 30 min.

[0102] The interfering substance is bovine albumin (fraction V) at a concentration of 0.30 g / l. This constitutes the cleanliness requirement.

[0103] The tests are incubated at a temperature of 30°C for a period of 2 days.

[0104] Results The 2% diluted formulation exhibits fungicidal activity against the strain Aspergillus niger after a contact time of 30 min at a temperature of 20°C and under clean conditions (0.30g / l of bovine albumin).

[0105] The results obtained for C . albicans And A . Niger are listed in the table below. Table 1. Fungicidal activity tests according to EN1650, dirty (3 g / L BSA) and clean (0.3 g / L BSA) conditions, 20°C Strain Tested batch Test concentration (w / w) Contact time Log reduction Fungicid activity BSA (g / L) Candida albicans 0182 / 2013 2% 5 min R>4, 22 YES 3 1,5% 5 min R>4, 22 YES 3 1,2% 5 min R > 4,07 YES 3 1% 5 min R<2, 70 NO 3 Aspergillus niger 0346A / 2013 2% 30 min R = 4,125 YES 0,3 0395A / 2013 2% 15 min R = 4,25 NO 0,3 3. Viruses - Rotavirus and Adenovirus

[0106] This virucidal efficacy test is carried out according to the methodology of the standard NF EN 14476+A1 (January 2007) on two viruses. has) Rotavirus

[0107] The first virus tested was a Rotavirus (strain ATCC VR-1290) and the tests were conducted on 3 samples of ready-to-use disinfectant product, according to the formulation diluted to 2% in water.

[0108] Method : The test is performed at 20°C (± 1°C). The contact time is 15 minutes. The samples are diluted in sterile distilled water. The interfering substance is bovine albumin (BSA 3.00 g / l).

[0109] The viral strain was tested on MA-104 cells at 36.5°C under 5% CO2.

[0110] The technique for stopping the virucidal action involves adding a solution containing Na₂CO₃ at a concentration of 70 g / L, pH 10.85, with 10 mL of the stopping solution per 10 mL of the test solution. This solution allows the disinfectant's action on the virus to be stopped and the efficacy of the tested biocidal formulation to be measured.

[0111] The virus is titrated in PFU / ml (plaque-forming units), which is the number of infectious viral particles per ml and more commonly the logarithm of the viral titer.

[0112] Results The three samples of diluted biocide formulation have virucidal activity on the Rotavirus strain at a concentration of 2% for 15 min of contact. b) Adenovirus

[0113] The second virus tested is an Adenovirus (adenoid strain 75 ATCC VR-5).

[0114] The biocidal formulation used for the preparation of the diluted composition according to the invention is diluted to 2% in water and the test method is the same as that used on Rotavirus and described above.

[0115] However, the contact time is 60 minutes.

[0116] The viral strain was tested on HEp-2 cells at 36.5°C under 5% CO2.

[0117] ResultsThe three samples of diluted biocidal formulation according to the invention have virucidal activity on the Adenovirus strain at a concentration of 2% for 60 min of contact.

[0118] The results obtained for the virucidal activity tests are summarized in the table below: Table 2. Virucidal activity tests according to EN 14476+A1, clean conditions (0.3 g / L BSA), 20°C viral strain Tested batch Test concentration (w / w) Contact time Log reduction Virucidal activity BSA (g / L) Rotavirus strain ATCC TVR - 1290 0212 / 2013 2% 15 min R = 4,25 YES 0,3 0226 / 2013 2% 15 min R = 4,375 YES 0,3 0227 / 2013 2% 15 min R = 4, 125 YES 0,3 Adenovirus adenoid strain 75 ATCC VR-5 0395A / 2013 2% 60 min R = 4,125 YES 0,3 0406A / 2013 2% 60 min R = 4,25 YES 0,3 0406B / 2013 2% 60 min R = 4,125 YES 0,3

[0119] It has also been shown that the present biocidal formulation is effective at a concentration of 1% against enveloped viruses with DNA genomes, such as those belonging to the family of Herpesviridae. In addition, the biocidal formulation is also effective at 1% against influenza viruses. 4. Bacteria

[0120] This quantitative suspension test for evaluating the bactericidal activity of the formulations used for preparing the diluted composition according to the present invention is carried out by a Microbiology Laboratory.

[0121] Method: A dilution / neutralization method is applied to a formulation used to prepare the diluted composition according to the invention. The neutralizer consists of sodium chloride (0.85% w / v), Na₂CO₃ (0.1%), and distilled water. This dilution / neutralization method stops the biocidal action of the product at the end of the tested contact time.

[0122] The The formulation used for preparing the diluted composition according to the invention is diluted in physiological saline at the following concentrations: 0.5%, 1.2%, and 1.5%. The 0.5% dilution does not represent a composition according to the invention.

[0123] The tests are performed on strains of Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC 10536), Enterococcus hirae (ATCC 10541) and Pseudomonas aeruginosa (ATCC 15442) at a temperature of 20°C (± 1°C). The contact time is limited to 5 minutes (± 10 seconds).

[0124] The interfering substance is bovine albumin (fraction V) at a concentration of 3.00 g / l. This constitutes the soiling condition.

[0125] Incubation is carried out at a temperature of 37.0°C (± 0.2°C) for 24 hours.

[0126] Results: According to the indications of standard NF EN 1276 (October 1997), a concentrated disinfection formula according to the formulation of the present invention exhibits bactericidal activity under dirty conditions (3.00 g / l bovine albumin) after 5 minutes of contact at 20°C against the 4 reference strains tested at a test concentration of 1%. The limiting microorganism is Staphylococcus aureus (ATCC 6538).

[0127] The results of the bactericidal tests are shown in Table 3 below.

[0128] Tests are also carried out according to a standardized method (EN13697) in order to demonstrate the bactericidal and / or yeasticidal effect by application on surfaces.

[0129] The control surfaces used are stainless steel discs onto which a solution of bacteria and / or yeast is deposited. Specifically, these are the microbial strains described above. The discs are then immersed in the biocide solution at 20°C (+ / - 0.2°C) for a contact time of 5 minutes. The residual microbial concentration is determined after incubation at 37°C (+ / - 2°C) for bacteria or at 25°C (+ / - 0.2°C) for yeast.

[0130] Results: According to the indications of standard EN 13697 (November 2001), a formula exhibits bactericidal and yeasticidal activity under dirty conditions (3.00 g / l of bovine albumin) after 5 minutes of contact at 20°C with respect to the 4 reference bacterial strains tested and the yeast strain for the test concentration of 1%. 5. Biodegradability

[0131] This test is an evaluation in aqueous medium of the ultimate aerobic biodegradability of a sample of the concentrated formulation.

[0132] This test is performed according to OECD Method 301 A using a sample containing 10 mg / L of dissolved organic carbon (DOC). The rate of DOC disappearance is monitored over a 28-day period in the "Concentrated Disinfection" test at 10 mg / L DOC. Physicochemical analysis is performed according to ASTM D5291.

[0133] There figure 1 The attached graph shows the degradation curves of dissolved organic carbon after 28 days. The reference substance is sodium acetate. The sample labeled "concentrated disinfection" corresponds to the tested biocide formulation described above, and the sample "inhibition test" corresponds to a mixture of the formulation and sodium acetate. Results :

[0134] The test is considered valid because: The percentage of degradation of the reference substance (sodium acetate) is greater than 70% (99%) on the fourteenth day; The difference between the COD measurement values ​​of the product studied is less than 20% at each sampling; The degree of COD elimination in the inhibitor test, containing the test compound and the reference substance, being greater than 35% after 14 days (98%), the sample is not considered toxic with respect to seeding. Conclusion:

[0135] The evaluation in aqueous media of the "ultimate" biodegradability of the sample tested, according to OECD guideline 301 A, gives the following results: The maximum degree of biodegradation of the sample at 10 mg / l of COD is equal to 100% after 28 days of incubation; The biodegradation time (corresponding to 90% of the maximum biodegradation rate) is approximately 25 days; According to OECD guideline 301 A, the tested sample "Concentrated Disinfection" is considered to be readily biodegradable (the COD reduction threshold level of 70% being acquired within a 10-day interval after 10% biodegradability is reached (94% at the end of the 10-day interval on the 11th day of testing).

[0136] The measured degree of biodegradability takes into account the entire formulation: indeed, there are no degradation byproducts because the formulation is composed exclusively of carbon, oxygen, and hydrogen atoms derived from plant extracts. This is a true measure of ultimate biodegradability.

Claims

1. Aqueous liquid composition with virucidal effect against virus strains belonging to the adenovirus family or with virucidal effect against rotavirus or with fungicidal effect against fungi belonging to the species Aspergillus niger, said composition comprising a biodegradable concentrated biocidal formulation, comprising compounds obtained from renewable plant resources, said concentrated biocidal formulation being characterised in that it comprises at least the following compounds: - at least lactic acid in a proportion of between 76 and 98% by mass relative to the total mass of the formulation; - at least one anionic surfactant consisting of an alkyl carboxylate selected from sodium caprate and sodium caprylate in a proportion of between 2 and 24% by mass relative to the total mass of the formulation; said biocidal formulation being present, in said aqueous liquid composition, in a proportion of between 1 and 2% by mass relative to the total mass of said composition, the remainder of said composition being water.

2. Aqueous liquid composition according to the preceding claim, wherein said biocidal formulation is present in a proportion of between 1.5 and 2% relative to the total mass of said aqueous liquid composition.

3. Aqueous liquid composition according to any one of the preceding claims, wherein said biocidal formulation is present in a proportion equal to 2% relative to the total mass of said aqueous liquid composition.

4. Aqueous liquid composition according to any one of the preceding claims, wherein said concentrated biocidal formulation comprises between 76 and 92% by mass of lactic acid and between 3 and 15% by mass of said anionic surfactant consisting of an alkyl carboxylate selected from sodium caprate and sodium caprylate.

5. Aqueous liquid composition according to any one of the preceding claims, wherein said biocidal formulation comprises between 5 and 10% by mass of anionic surfactant consisting of an alkyl carboxylate selected from sodium caprate and sodium caprylate.

6. Aqueous liquid composition according to any one of the preceding claims, wherein said concentrated biocidal formulation also comprises a nonionic surfactant in a proportion of between 2 and 15% by mass relative to the total mass of the formulation, said nonionic surfactant being chosen from glycosides and alkyl polyglycosides comprising a number of carbon atoms between 5 and 20, the hydrophilic group being composed of pentose or hexose.

7. Aqueous liquid composition according to the preceding claim, wherein said concentrated biocidal formulation comprises a nonionic surfactant with between 5 and 18 carbon atoms.

8. Aqueous liquid composition according to any one of claims 6 or 7, wherein said concentrated biocidal formulation comprises a nonionic surfactant selected from octyl glucosides, decyl glucosides, lauryl glucosides, coco glucosides, D-glucopyranose, D-xylopyranose or C10-C16 alkyl polyglycosides.

9. Aqueous liquid composition according to any one of the preceding claims, wherein said concentrated biocidal formulation also comprises a natural fragrance in a proportion of between 0.01 and 10% by mass relative to the total mass of the formulation, said fragrance being chosen from essential oils, plant essences and plant extracts.

10. Aqueous liquid composition according to any one of the preceding claims, wherein said biocidal formulation also comprises a chelating agent in a proportion of between 0.1 and 10% by mass relative to the total mass of the composition, said chelating agent being chosen from sorbic acid derived from rowan, oxalic acid derived from plant roots or rhizomes, sequestering monomers or polymers, and amino acids.

11. Aqueous liquid composition according to any one of claims 1, 2 and 4 to 10, wherein the anionic surfactant of said concentrated biocidal formulation consists of sodium caprylate.

12. Aqueous liquid composition according to any one of the preceding claims, wherein said concentrated biocidal formulation comprises: - 2.4% C10-C16 alkyl polyglycoside type nonionic surfactants; - 5% fatty acid salt type anionic surfactants of the sodium caprylate type; - 91% lactic acid; - 1.6% water, said biocidal formulation being present, in said aqueous liquid composition, in a proportion equal to 2% by mass relative to the total mass of said composition, the remainder of said composition being water.

13. Non-therapeutic method for inactivating rotavirus, characterised in that the aqueous liquid composition according to the preceding claim is applied for a contact time of 15 min at a temperature of 20°C (+ / - 1°C).

14. Non-therapeutic method for inactivating virus strains belonging to the adenovirus family, characterised in that the aqueous liquid composition according to claim 12 is applied for a contact time of 60 min at a temperature of 20°C (+ / - 1°C).

15. Non-therapeutic method for inactivating strains of a fungus belonging to the Aspergillus niger strain, characterised in that the aqueous liquid composition according to claim 12 is applied for a contact time of 30 min at a temperature of 20°C (+ / -1°C).