SYSTEM FOR TRIPLE COMBINATION CRISPR SCREENS FOR ANALYSING TARGET INTERACTIONS AND METHODS THEREOF OF IT
Patent Information
- Application Number
- DE602021044194
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Filing Date
- 2021-04-14
- Publication Date
- 2025-12-10
- Estimated Expiration
- 2041-04-14
AI Technical Summary
Existing methods struggle to systematically screen high-order genetic and drug combinations due to unanticipated synergy or antagonism, leading to limited discovery of effective drug combinations, especially those comprising three or more drugs, and are prohibitively expensive with microplate arrays and RNA interference systems.
A CRISPR-based multi-gene knockout screening system using barcoded high-order combinatorial guide RNA libraries, assembled via CombiGEM-CRISPR v2.0, enables high-throughput screening of three-way synergistic therapeutic target combinations by modifying promoter sequences and gRNA scaffolds to minimize lentiviral vector recombination.
The system efficiently identifies synergistic drug combinations for diseases like cancer and Parkinson's disease, overcoming the limitations of previous methods by reducing sequencing costs and experimental complexity, and validating effective three-way drug regimens.