PROTEIN STABILITY TEST
Patent Information
- Application Number
- DE602022019563
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2021-12-15
- Filing Date
- 2022-12-14
- Publication Date
- 2025-08-13
- Estimated Expiration
- 2042-12-14
AI Technical Summary
Existing methods for detecting protein-ligand binding, particularly in a cellular context, are laborious, require multiple pipetting steps, involve high sample consumption, and are not suitable for high-throughput screening due to the need for temperature control and separation of native and denatured protein fractions.
A protein stability assay (PSA) that uses chemical denaturing agents at a constant temperature to determine the proportion of folded and denatured protein states, eliminating the need for temperature changes and vessel transfers, suitable for high-throughput screening.
The PSA provides a sensitive and robust method for detecting ligand-target engagement, simplifying the screening process and reducing complexity, making it suitable for large-scale applications.