Concentrate for a cleaning solution

Abstract

The invention relates inter alia to a concentrate (10) for a cleaning fluid (11) circulating in a cleaning system (18, 31) for removing, in particular for the residue-free removal of, oil- or lubricant-containing contaminants from the surfaces of workpieces (33a, 33b, 33c), wherein the concentrate comprises two different, self-separating phases (12, 13), wherein the first phase (12) comprises a solution containing bacterial spores, and wherein the second phase (13) comprises a mixture comprising at least the following components: i) non-ionic surfactants, ii) hydrotropes (clarifiers), iii) complexing agents, iv) preservatives, wherein the first phase (12) and / or the second phase (13) comprises at least one membrane lipid, in particular a glycolipid.

Description

[0001] The invention relates initially to a concentrate for a cleaning solution.

[0002] The concentrate serves to provide a cleaning solution. The cleaning solution is an agent for emulsifying, degreasing, and removing oil and / or lubricants from surfaces. In particular, the invention relates to concentrates, cleaning solutions, and methods for producing these cleaning solutions, which can remove contamination in the form of oil-containing or lubricant-containing substances and surfaces of materials without leaving any residue or with substantially no residue. This substantially residue-free removal is achieved using oil-degrading bacteria.

[0003] According to a key aspect of the invention, the concentrate according to the invention is produced as a 2-phase concentrate and is used in washbasins based on bacterial spores and glycolipids in a ratio with water, in particular between 1:3 and 1:6.

[0004] The concentrate according to the invention contains, in particular, glycolipids and a mixture of several surfactants in or with a spore solution.

[0005] The concentrate and / or the finished cleaning solution may also contain ethoxylates, mixtures of spore solutions, glycolipids and, in particular, suprammolecular complexes consisting of rhamnolipids, alginates, pyoverdines and water. State of the art:

[0006] Due to the manufacturing process, workpiece surfaces often contain greases and oils, drilling solutions, and other pigment-like contaminants. These surfaces must be cleaned to, for example, improve adhesion during further processing.

[0007] It is known to provide washbasins for this purpose, in which organic and inorganic solvents are used. These washbasins are sometimes operated with oil separators or with oil-degrading bacteria to increase the service life of the washing solutions used.

[0008] Various synthetic surfactants are added to the washbasins. Surfactants contain a hydrophilic (polar) and a hydrophobic (nonpolar) part. Due to this amphiphilic nature, they are surface-active in various solvents, especially water. This means they accumulate at the interfaces of the aqueous phase. The surfactant molecules used thus form monomolecular films at the interfaces, which can significantly influence the properties of the systems.

[0009] Interface adsorption leads to the following effects: Reduction of the interfacial tension between water and the adjacent phase; change in the wetting properties between water and solids; formation of electrical double layers at the interfaces.

[0010] For cleaning workpieces, washbasins are known in the prior art, such as those offered by the company Biocircle in Gütersloh, Germany. In these washbasins, the greases and oils are emulsified by surfactants or dissolved and washed away by solvents.

[0011] After cleaning or removing the workpiece, organic compounds can lead to re-greasing if they reach a certain concentration. This is comparable to dishes taken from heavily soiled dishwater, which are still coated with a film of grease. After a certain time, the grease absorption capacity of the wash bath is exhausted, and a film of grease forms on the water's surface. To achieve high-quality degreasing, it is essential to prevent re-greasing and re-contamination of the workpiece. This can be remedied by connecting a grease separator to the system or by adding grease- and oil-degrading microorganisms to the wash solution in the wash basin.

[0012] A wide variety of microorganisms are capable of breaking down the washed-off contaminants. These include, for example, gram-positive species such as Arthrobacter, Bacillus, and Nocardia, and gram-negative species such as Flavobacterium, Enterobacter, Escherichia, Pseudomonas, and other genera.

[0013] The decomposition of fats and oils (lipids) occurs in several steps. Hydrocarbon breakdown takes place within the cell. Water-soluble hydrocarbons are absorbed directly through the cell membrane, while water-insoluble hydrocarbons are deposited on the lipid-soluble cell wall. Surfactants synthesized by microorganisms can emulsify the lipid-water mixture. This increases the surface area of ​​the phase interface and thus improves the efficiency of the decomposition process. The actual lipid breakdown occurs intracellularly.

[0014] Most organisms incorporate molecular oxygen into fats and oils via an enzymatically catalyzed oxidation reaction in order to utilize them in their metabolism. For this reason, the breakdown of aliphatic hydrocarbons, such as paraffins and aromatic hydrocarbons, is most efficient in the presence of oxygen. Therefore, these systems are continuously supplied with oxygen. The oxygen supply to the microorganisms is achieved by blowing in air.

[0015] The washbasins used in this way operate on the principle of a bioreactor. They are operated at a process temperature of > 42 °C to prevent the proliferation of pathogenic germs. Additionally, higher temperatures reduce the viscosity of the oils and fats, accelerate chemical reactions, and thus intensify the process.

[0016] The washing solution can be adjusted to a pH of 8.5, which is tolerable for microorganisms, for example, using a phosphoric acid solution. This adjusted pH also prevents the proliferation of pathogens in the cleaning bath. At the same time, the phosphoric acid serves as an additional phosphorus source for the microorganisms used.

[0017] The washing or cleaning solution in the prior art must not exceed a predetermined service life and is then replaced. For this purpose, the solution is transported from the factory of a cleaning solution manufacturer to the washing station, which is usually installed at the work site. The cleaning fluid contained in the washing station is disposed of after the predetermined service life has been reached, and the washing station is refilled with a new and fresh cleaning solution.

[0018] WO 2005 / 040320 discloses a cleaning solution containing bacterial spores, non-ionic surfactants, hydrotropes and complexing agents. Purpose of the invention:

[0019] Based on the described prior art, the object of the invention is to provide a way to improve the method used in the prior art.

[0020] The invention solves this problem initially with claim 1.

[0021] A concentrate for a cleaning solution is proposed. The concentrate comprises two phases.

[0022] The first phase comprises a spore solution, where the spore solution consists of bacterial spores. The second phase comprises nonionic surfactants, including ethoxylated C10 alcohol with ≥ 2.5 to ≤ 4 EO units and ethoxylated C10 alcohol with >5 to ≤ 10 EO units. The two phases are self-separating from each other.

[0023] In one embodiment of the invention, the two different phases have different pH values. The pH values ​​can range between 6.5 and 9.5.

[0024] According to the invention, the concentrate can be filled at the manufacturer's plant and transported as a concentrate to the place of use. Only there is it diluted with water and mixed with water to form a cleaning solution.

[0025] After dilution with water, the phases are mixed together and can no longer separate from each other.

[0026] According to the invention, the concentrate is dilutable with water. The concentrate according to the invention can be diluted with tap water or demineralized water. Since water can be provided virtually anywhere in the world, the invention allows only the concentrate to be transported to the point of use, and existing water supplies at the point of use, for example, at the installation site of the washbasin, can be utilized. Due to transport as a concentrate, transport costs and transport volume can be reduced compared to the prior art.

[0027] The concentrate according to the invention comprises two self-separating phases. This allows all components required for the provision of a cleaning solution – with the exception of water – to be safely stored and transported over long periods of time.

[0028] Due to the special ingredients of the concentrate according to the invention, or the cleaning solution subsequently prepared, contaminants can be removed more effectively from the surfaces of the workpiece. Residues can also be dissolved more effectively. Finally, the service life of a cleaning or washing solution in a washbasin within a cleaning system is significantly increased with the concentrate according to the invention.

[0029] An advantage of the concentrate according to the invention is that the product, which is formulated as a two-phase concentrate, remains inactive until the bacteria used, which are present as spores in the highly concentrated form, are activated. Activation is achieved by diluting the concentrate with tap water or demineralized water, under the influence of temperature, particularly at a temperature above 40 degrees Celsius, and with the addition of oxygen. Producing the product as a two-phase concentrate offers economic and ecological advantages, including, among others, a reduction in storage, transport, and handling costs.

[0030] When used as a two-phase concentrate in combination with glycolipids in a closed system, significantly better results are achieved with a cleaning solution compared to the state of the art. This is reflected in increased service life, improved cleaning performance, and better solubilization of natural and synthetic fats and oils. The improved solubilization leads to better metabolism by the bacteria used, resulting in better overall results.

[0031] A further advantage of the invention is that the specially formulated nutrient solution, consisting of glycolipids and the inactive spore solution, allows the metabolism of the bacterial cells to be optimized by adding water, resulting in very little biomass accumulating in the washbasin. The organic waste, along with the introduced inorganic solids, can be removed from the system via an integrated filter.

[0032] The biomass can, for example, be sedimented via a separator, especially a lamella separator, drawn off, and dewatered periodically in a chamber filter press. The filter cake is then disposed of.

[0033] The concentrate according to the invention is particularly suitable for providing a cleaning solution for the essentially residue-free removal of oil- or lubricant-containing contaminants. The formulation "residue-free" or "essentially residue-free" allows, in accordance with the invention, for the accumulation of solids, e.g., metal shavings, but also flocculated biomass, namely, e.g., dead bacteria, which are separated and disposed of.

[0034] The concentrate according to the invention offers significant advantages with regard to reducing grease films in the washing solution and on the workpieces. This is primarily due to the additional enhanced degreasing performance of the spore solution, which is activated after dilution in combination with the glycolipids. Oily films are avoided in the wash basin itself. The quality of the wash water is maintained for a long period of up to several months. This, of course, requires regular filter maintenance, the addition of the wash water lost during washing, and regular replenishment of the concentrate according to the invention.

[0035] The concentrate according to the invention can, for example, comprise rhamnolipids or sophorolipids or a mixture thereof. Both of these substances are so-called biosurfactants.

[0036] These are defined in the usual way and in particular as follows: Definition of sophorolipids:

[0037] Sophorolipids are microbial biosurfactants of the glycolipid class, consisting of a hydrophobic fatty acid tail with 16 or 18 carbon atoms and a hydrophilic carbohydrate head, sophorose. Definition of rhamnolipids:

[0038] Rhamnolipids are biosurfactants belonging to the glycolipid group and are primarily produced by Pseudomonas aeruginosa (Abdel-Mawgoud et al., Introduction 11, 2011). They consist of one (mono-rhamnolipids) to two (di-rhamnolipids) rhamnose units and one to three β-hydroxy fatty acids. The β-hydroxy fatty acids can be saturated or unsaturated and have a chain length of 8 to 16 carbon atoms.

[0039] It is particularly advantageous to add rhamnolipids of the P. aeruginosa JRV-L strain to the products, as their cell-free culture fluid is capable of emulsifying various hydrocarbon agents with an emulsifying index E24 in the range of 60-80%. Another special feature of this compound is its ability to emulsify hydrocarbons at different pH values. This allows for the production of various products tailored to different types of soiling.

[0040] It is particularly advantageous to use the natural polymer alginate contained in rhamnolipid, with a molecular weight of 400-600 kDa, due to its high emulsifying activity. This allows the natural amphiphilic substances to develop their hydrophilic and hydrophobic properties, enabling them to bind at the interfaces between liquid phases with different degrees of polarity. This results in optimal emulsification of fats and oils and / or water and adhering impurities.

[0041] Phospholipids belong to the family of membrane lipids. These are phosphorus-containing lipids. A distinction is made between phosphoglycerides and sphingoglycolipids.

[0042] Alternatively or additionally to phospholipids, glycolipids can also be used as membrane lipids. These are phosphorus-free structural lipids.

[0043] The glycolipids mentioned as special membrane lipids according to claim 1 include the rhamnolipids and sophorolipids already mentioned, as well as, for example, rhamnoselipids, threhaloselipids and glycosyldiglycerides.

[0044] Glycolipids include, in particular, sodium surfactin, the salt of surfactin. This can be obtained commercially, for example, under the trade name Kaneka Surfactin from Kaneka Corporation, based in Osaka, Japan.

[0045] According to the invention, it is sufficient to add only minute quantities of these membrane lipids. Even extremely small amounts, sometimes as little as 0.01% in the concentrate, result in a significant reduction in surface tension.

[0046] If various membrane lipids are mentioned in the present patent application and proposed as components of the concentrate according to the invention, such as phospholipids or glycolipids, in particular rhamnolipids, the invention also includes the provision of a mixture of several of these different membrane lipids as components in the concentrate.

[0047] The concentrate according to the invention comprises a solution containing bacterial spores. For this purpose, for example, the bacterial solution commercially available from NobleBio BV in Oldenzaal, the Netherlands, under the product name UB2 Cultuur number 1200, can be considered.

[0048] As an example of a sophorolipoid usable according to the invention, the biosurfactant commercially available under CAS number 2568-33-4 under the registered trademark SOPHOCLEAN ®< from Impag Import GmbH in Offenbach is mentioned.

[0049] Alternatively, the substance SOPHOGREEN, which is available from the same source under CAS number 7732-18-5 and also belongs to the group of glycolipids, can be used.

[0050] Finally, sodium surfactin (CAS number 302933-83-1 / 24730-31-2), which is commercially available from the company Kaneka in Japan under the name KANEKA, can also be used.

[0051] Suitable rhamnolipids are also biosurfactants belonging to the glycolipid group. These can be obtained commercially, for example, under CAS number 4348-76-9.

[0052] It is particularly advantageous to use microbial biosurfactants as cosurfactants, such as glycolipids.

[0053] The concentrate according to the invention forms two phases, the lower phase being the bacterial spore solution and the upper phase being a surfactant solution.

[0054] According to an advantageous embodiment of the invention, the concentrate contains rhamnolipids.

[0055] According to an alternative advantageous embodiment of the invention, the concentrate according to the invention comprises sodium surfactin.

[0056] According to an alternative advantageous embodiment of the invention, the concentrate according to the invention comprises sophorolipoides.

[0057] According to an alternative advantageous embodiment of the invention, the concentrate according to the invention comprises a foam blocker.

[0058] Glycolipids, also called glycolipids or glycolipids, are phosphorus-free structural or membrane lipids (components of cell membranes) in which one or more mono- or oligosaccharides are glycosidically linked to a lipid molecule. The lipid consists of fatty acids linked to glycerol via ester bonds or to sphingosine via amide bonds. Glycolipids are found in all tissues, but exclusively on the outer surface of the lipid bilayer.

[0059] Anionic surfactants are surfactants that possess a negatively charged functional group. Like all surfactants, anionic surfactants consist of a polar and a nonpolar part. The nonpolar part is an alkyl group. The polar functional group is -COO⁻ (carboxylate), -SO₃⁻ (sulfonate), or -SO₄²⁻ (sulfate).

[0060] Nonionic surfactants, also known as niotenes, are surfactants that do not contain dissociable functional groups and therefore do not break down into ions in water. Like all surfactants, nonionic surfactants consist of a nonpolar and a polar part. The nonpolar part is usually a fatty alcohol (C12-C18) or octyl or nonylphenols. The polar groups are the hydroxyl group and the ether group. These groups are found in polyethylene glycol or monosaccharides.

[0061] Surfactants form association colloids with sparingly soluble substances in the form of micelles. Other substances, such as urea or N-methylacetamide, disrupt the water structure at the hydrophobic sites of the substance to be dissolved. A third possibility is the formation of mixed crystals.

[0062] According to claim 1, the concentrate according to the invention also comprises hydrotropes. Hydrotropes are agents that increase the water solubility of sparingly soluble organic compounds. They are therefore solubilizers.

[0063] Hydrotropes, as defined in the patent application, are also referred to as clarifying agents. These are substances that can eliminate turbidity.

[0064] The concentrate according to the invention also comprises at least one complexing agent.

[0065] Complexing agents are Lewis bases, i.e., chemical compounds or simple anions with lone pairs of electrons that form coordination compounds with metal ions or metal atoms as Lewis acids. They can, for example, mask (undesired) chemical properties of metal ions.

[0066] The concentrate according to claim 1 further comprises one or more defoamers.

[0067] Defoamers or antifoaming agents are chemical formulations with pronounced surface activity that are suitable for suppressing unwanted foam formation (e.g., in wastewater treatment, papermaking, washing machine processes, painting, and fermentation processes) or for destroying existing foam. A distinction is sometimes made between defoamers (which prevent foam formation or dissolve existing foam) and deaerators (which bring air bubbles to the surface more quickly). The similar term "foam inhibitor" refers almost exclusively to the field of food technology and describes substances or mixtures with essentially the same effect. Examples include mono- and diglycerides of fatty acids and dimethylpolysiloxane.

[0068] CN102330105A discloses a cleaning fluid that contains neither hydrotropes nor a solution of bacterial spores and, furthermore, does not include a membrane lipid. Finally, this document also does not disclose a concentrate comprising the nonionic surfactants mentioned in claim 1.

[0069] WO 2016 / 097857A1 discloses a cleaning fluid that does not contain a hydrotrope. The document also does not disclose the special non-ionic surfactants listed in claim 1.

[0070] According to the invention according to claim 1, the invention relates to a concentrate for a cleaning fluid.

[0071] The invention also relates to a cleaning fluid according to claim 4.

[0072] Starting from the prior art described above, the object of the invention is again to provide a cleaning fluid that can improve the cleaning process according to the prior art.

[0073] The invention solves this problem with the features of claim 4.

[0074] To avoid repetition, reference is made to the previous explanations regarding the understanding of these features and advantages.

[0075] The cleaning fluid according to the invention comprises a concentrate and water. The amount of water corresponds to approximately one to ten times the amount of concentrate.

[0076] According to another aspect, the invention relates to a method according to claim 7.

[0077] Starting from the prior art described at the outset, the object of the invention is to provide a method that is improved compared to the prior art method.

[0078] The invention solves this problem with the features of claim 9.

[0079] The method according to claim 7 enables, according to step b), the preparation of the cleaning solution by diluting the concentrate with water immediately before or during (or equally shortly after) the introduction of the concentrate into a cleaning system, i.e., into a receiving basin for the cleaning fluid in the cleaning system. This avoids the transport volume of water components. It is sufficient to transport the concentrate only once to the point of application of the cleaning fluid.

[0080] To avoid repetition, reference is made to the previous statements.

[0081] According to an advantageous embodiment of the invention, the membrane lipid is a glycolipid belonging to the group of the following substances: a) Rhamnose lipids or rhamnolipids b) Sophorose lipids or sophorolipids c) Trehalose and other mycolic acid-containing glycolipids d) Cellobiose and mannosylerythritol lipids.

[0082] According to an advantageous embodiment of the invention, the concentrate or cleaning solution comprises at least the following components: i) Non-ionic surfactants comprising ethoxylated C10 alcohol with ≥ 2.5 to ≤ 4 EO units and ethoxylated C10 alcohol with >5 to ≤ 10 EO units ii) Hydrotropes (clarifiers) iii) Complexing agents iv) Preservatives.

[0083] According to another aspect, the invention relates to a method according to claim 9.

[0084] Again, the invention is based on the objective of improving the prior art method.

[0085] The invention solves this problem with the features of claim 9.

[0086] To avoid repetition, reference is made to the previous statements.

[0087] The invention further relates to a container according to claim 10.

[0088] To avoid repetition, reference is made to the previous statements.

[0089] Finally, the invention relates to the use of a concentrate according to claim 11.

[0090] To avoid repetition, reference is made to the previous statements.

[0091] Further advantages of the invention will become apparent from the uncited dependent claims as well as from the following description of the embodiments shown in the drawings and the embodiments described in the following text.

[0092] The drawings show: Fig. 1 shows a partially cutaway schematic view of a first embodiment of a container filled with a first embodiment of a concentrate according to the invention, which has two phases; Fig. 2 shows an embodiment of a cleaning solution according to the invention in a basin provided by a washbasin; and Fig. 3 shows an embodiment of a container connected to the washbasin. Figs. 2 alternative cleaning system.

[0093] Exemplary embodiments of the invention are described in the following description of the figures, also with reference to the drawings. For the sake of clarity, identical or comparable parts, elements, or areas are designated with the same reference numerals, sometimes with the addition of lowercase letters, even where different embodiments are concerned.

[0094] Features described only in relation to one embodiment can also be provided in any other embodiment of the invention. Such modified embodiments are included in the invention, even if they are not shown in the drawings.

[0095] All disclosed features are essential to the invention.

[0096] An embodiment of a concentrate according to the invention is described in Figs. 1 Designated with reference numeral 10, the concentrate is intended to be diluted with water in a ratio of 1 to 10, advantageously in a ratio of 1 to 6 to 1 to 3, to form a cleaning fluid 11, as described in Figs. 2 is shown.

[0097] The starting point is concentrate 10 according to Figs. 1, which has two separate phases 12 and 13. The first phase 12 is separated from the second phase 13 by a separating layer 14.

[0098] The first phase 12, the floating phase at the bottom, comprises a bacterial spore solution. The upper phase 13 comprises a surfactant mixture. A thin separating layer 14 can form during the self-separation of the two phases 12 and 13.

[0099] Figs. 1 Figure 1 shows an embodiment of a container according to the invention in the form of a canister 15. An outlet 16 is indicated above a conventional canister, which can be closed by a lid 37. Advantageously, the canister 15 is not completely filled, but a certain gap of air 36 remains after filling.

[0100] The concentrate 10 according to the invention can be placed in a washbasin 18 according to Figs. 2They are inserted and placed in a basin 17 to receive a cleaning solution 11. The cleaning solution 11 comprises water and concentrate 10.

[0101] The washbasin 18 includes a pump 19, shown only schematically, e.g., a circulation pump, which can ensure the circulation of the cleaning fluid. The circulation pump 19 includes a suction side 20 and a pump outlet 21.

[0102] Advantageously, a filter element 28 is arranged on the intake side 20.

[0103] The pump outlet has a section through which the cleaning fluid flows back into basin 17, thus achieving circulation.

[0104] The pump 19 can supply the cleaning fluid to a hand basin 25 via a further pipe section 22. A feed 23 is indicated for this purpose, as well as an additionally or alternatively indicated brush feed 24.

[0105] The diagram only schematically suggests Figs. 2 A type of Y-connector is attached to the outlet side of pump 19. Of course, other arrangements can also be made to achieve, on the one hand, circulation of the cleaning fluid 11 in the basin 17, and on the other hand, to pump cleaning fluid to the hand basin 25.

[0106] The washbasin 18 according to Figs. 2The device features a hand basin 25 into which workpieces 33 with oily surface contamination can be placed. Cleaning fluid 11, indicated by drops 27c and 27d, can be manually fed to the workpiece 33 using the feeder 23 or brush feeder 24. The cleaning fluid washes the surface of the workpiece and removes the contamination. The contamination is conveyed to an outlet 39 of the hand basin 25 and passes through a solids filter 26 for coarse particles, such as metal shavings. From the filter 26, the cleaning fluid, indicated by drops 27a and 27b, can drip back into the basin 17.

[0107] The cleaning plant 18 according to Figs. 2Furthermore, it includes a heating element 29 to temper the cleaning fluid 11, preferably to a temperature above 42°C. This keeps the bacteria in an activated state and also eliminates unwanted germs.

[0108] Finally, an air supply 30 is indicated, which supplies the cleaning fluid 11 within the basin 17 with oxygen.

[0109] A valve 38 is indicated on line 22, which can be operated manually or automatically, for example.

[0110] An alternative to a cleaning system 31 is in Figs. 3As shown, a transport device 34 passes through the basin 17, which contains the cleaning fluid 11. The transport device 34 can include a receiving device 32 in which a plurality of workpieces 33a, 33b, 33c can be accommodated. The loading of the transport device 34 with workpieces 33a, 33b, 33c can, for example, take place at a loading station 41, which is only indicated. The transport device 34 can then pass through the basin 17, whereby the workpieces 33a, 33b, 33c are wetted with cleaning fluid. The receiving device 32 can, for example, be designed as a wire mesh box to allow the cleaning fluid to pass through.

[0111] After passing through the basin 17 containing the cleaning fluid 11, the receiving device 32 can be unloaded of the cleaned workpieces at an unloading station 42. The transport device 34 can then be moved along a transport track 43.

[0112] The cleaning system 31 also according to Figs. 3 It has a heater 29, a pump 19 and an air supply 30. Examples of implementation:

[0113] The following is an exemplary method for producing an embodiment of a stable two-phase concentrate according to the invention: a) The spore solution is added to a stirred tank while stirring at a maximum speed of 30 revolutions per minute. During the entire manufacturing process, the following additives are added while stirring continuously. b) The glycolipid (rhamnolipid; and / or surfactin; and / or sophorolipoids) is added. c) A first surfactant alcohol ethoxylate C10⁻⁴ EO is added. d) A second surfactant alcohol ethoxylate C10⁻⁵,⁵ EO is added. e) A hydrotropic alkyl amide ethoxylate C12-C14 is added. f) The complexing agent consisting of glutamic acid, N,N-diacetic acid, tetrasodium salt is added. g) The preservative, 2-phenoxyethanol, '-n-butylbenzo[d]isothiazol-3-one, N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine, is added. h) The pH is adjusted to a value between 8 and 8.5 using citric or phosphoric acid. i) After adjusting the pH, the mixture is stirred for approximately 20 minutes.j) While stirring constantly, the concentrate is poured into the appropriate containers, for example, a 20-liter container. k) Within approximately 30 minutes, the two phases will have formed. l) To obtain a cleaning solution, the concentrate thus obtained is diluted with water in a ratio of 1:4, 1:5, or, depending on the degree of soiling, between 1:3 and 1:6.

[0114] According to one variant, the manufacturing process is carried out at room temperature.

[0115] In the first embodiment of a method according to the invention for producing an embodiment of a concentrate according to the invention described below, the concentrate comprises at least eight components: A first component can be provided by a rhamnolipid, or by surfactin, or by sophorolipid. Alternative 1:

[0116] Rhamnolipids can reduce the surface tension of water from 72 mN / m to values ​​below 30 and the interfacial tension of water / OI systems from 43 mN / m to values ​​< 1 mN / m. Rhamnolipids can be used in the concentrate according to the invention at a concentration of < 1%. Alternative 2:

[0117] Surfactin is a cyclic lipopeptide consisting of seven amino acids and various β-hydroxy acids (C13-C5; main component: 3-hydroxy-13-methylyristic acid). At a concentration of 25 mg / L, surfactin reduces the surface tension of water from 72 mN / m to 27 mN / m and the interfacial tension in the water / n-hexadea system from 43 mN / m to below 1 mN / m. In one embodiment of the invention, surfactin can be used at a concentration of less than 1% in the concentrate. 3. Alternative:

[0118] Sophorolipoides are fermentation products of glucose and rapeseed oil fatty acid methyl esters with yeast.

[0119] The second component can be provided by a surfactant alcohol ethoxylate C10 4 EO at a concentration of >5%.

[0120] The third component comprises a surfactant alcohol ethoxylate C10 4,5 EO at a concentration >10.0%.

[0121] The fourth component comprises a hydrotropic alkyl amide ethoxylate C12 - C14 in a concentration of >5%.

[0122] The fifth component comprises a complexing agent consisting of glutamic acid, N,N-diacetic acid, tetrasodium salt in a concentration > 20%.

[0123] The sixth ingredient comprises a preservative, 2-phenoxyethanol, "-n-Butyl-benzo[d]isothiazol-3-one, N-(3Aminopropyl)-N-dodecylpropane-1,3-diamine in a concentration of < 5%.

[0124] The seventh component comprises a pH-regulating agent, such as phosphoric acid or citric acid, in a concentration of > 2%.

[0125] The eighth component comprises a spore solution at a concentration of > 40%.

[0126] Additionally, in the case of undesired foam formation in the washbasin, the invention may provide that the concentrate includes a defoamer, for example silicone-based, in particular in a concentration of less than < 1%.

[0127] Enclosed are some further exemplary embodiments of recipes for the production of a concentrate according to the invention: First embodiment of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 0,05% Citric acid 2,80% 100,00% Second example of a recipe: UB2 Culture Number 1200 40,00% Dissolvine GL 47 S 25,00% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 1,20% pH Value regulating medium Citric acid, Lactic acid; Phosphoric acid 2,80% 100,00% Third example of a formulation: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Rhamnolipid 0,05% Citric acid 2,80% 100,00% Fourth example of a recipe: UB2 Culture Number 1200 40,00% Dissolvine GL 47 S 25,00% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sodium Surfactin 1,20% Citric acid 2,80% 100,00% Fifth example of a recipe: UB2 Culture Number 1200 40,00% Dissolvine GL 47 S 25,00% Berol 360 7,50% Berol 366 13,30% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 0,40% Citric acid 2,80% 100,00% Sixth example of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 0,05% Citric acid 2,80% 100,00% Seventh example of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Surfactin Sodium 0,05% Citric acid 2,80% 100,00% Eighth example of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 5,50% Berol 366 14,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 0,05% Citric acid 2,80% 100,00% Ninth example of a recipe: UB2 Culture Number 1200 35,87% Dissolvine GL 47 S 30,28% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 0,05% Citric acid 2,80% 100,00% Tenth example of a recipe: UB2 Culture Number 1200 37,00% Dissolvine GL 47 S 25,00% Berol 360 7,50% Berol 366 15,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 1,20% pH Value regulating medium Citric acid, Lactic acid; Phosphoric acid 2,80% 100,00% Eleventh example of a recipe: UB2 Culture Number 1200 44,87% Dissolvine GL 47 S 23,28% Berol 360 5,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Rhamnolipid 0,05% Citric acid 2,80% 100,00% Twelfth example of a recipe: UB2 Culture Number 1200 40,00% Dissolvine GL 47 S 25,00% Berol 360 5,50% Berol 366 14,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sodium Surfactin 1,20% Citric acid 2,80% 100,00% Thirteenth example of a recipe: UB2 Culture Number 1200 40,00% Dissolvine GL 47 S 25,00% Berol 360 7,50% Berol 366 15,30% Berol Surfboost AD15 5,50% Parmetol BPX 3,50% Sophoclean 0,40% Citric acid 2,80% 100,00% Fourteenth example of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 5,50% Berol 366 16,50% Berol Surfboost AD15 5,50% Parmetol BPX 3,50% Rhamnolopide 0,05% Citric acid 2,80% 100,00% Fifteenth example of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,23% Berol 360 7,50% Berol 366 12,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Surfactin Sodium 0,05% Sophoclean 0,05% Citric acid 2,80% 100,00% Sixteenth example of a recipe: UB2 Culture Number 1200 40,87% Dissolvine GL 47 S 25,28% Berol 360 4,50% Berol 366 14,50% Berol Surfboost AD15 7,50% Parmetol BPX 3,50% Sophoclean 1,05% citric acid 2,80% 100,00%

[0128] In the above-mentioned embodiments, the designations Berol 360, Berol 366 and Berol Surfboost AD15 refer to embodiments for various surfactants.

[0129] The following CAS data is provided: Berol 360: CAS: 26183-52-8 Alcohol, C10, ethoxylated (≥2.5 - ≤4 EO) Berol 366: CAS: 26183-52-8 Alcohol, C10, ethoxylated (>5 - ≤10 EO) Berol Surfoost AD15: Mixture of wetting agents, C12-C14 amide ethoxylate

[0130] In the above examples, the compound Dissolvine is given as an example of a complexing agent.

[0131] The following CAS data is given: Dissolvine GL 47: CAS No: 51981-21-6 L-Glutamic acid, N,N-diacetic acid tetrasodium salt

[0132] In the above examples, the ingredient Parmetol is also given as an example of a preservative. For this, we refer to the following substance information: Parmetol BPX: PHENOXYETHANOL, 2-n-Butylbenzo[d]isothiazol-3-one, N-(3-Aminopropyl)-N-dodecylpropane-1,3-diamine

Claims

1. Concentrate (10) for a cleaning fluid (11) circulating in a cleaning system (18, 31) for removal, in particular for residue-free removal, of oil or lubricant contamination on surfaces of workpieces (33a, 33b, 33c), wherein the concentrate comprises two different phases (12, 13), self-separating from each other, wherein the first phase (12) comprises a solution with bacterial spores, and wherein the second phase (13) comprises a mixture, which comprises at least the following components: i) non-ionic surfactants, comprising ethoxylated C10 alcohol with ≥ 2.5 to ≤ 4 EO units and ethoxylated C10 alcohol with > 5 to ≤ 10 EO units ii) hydrotropes (clarifying agents) iii) complexing agent iv) preservatives wherein the first phase (12) and / or the second phase (13) has at least one membrane lipid, in particular a glycolipid.

2. Concentrate according to claim 1, characterised in that the first phase (12) comprises an anionic surfactant.

3. Concentrate according to claim 1 or 2, characterised in that the membrane lipid is a glycolipid, which originates from the group of the following substances: a) rhamnolipids b) sophorolipids c) trehalose and other mycological acidic glycolipids d) cellobiose lipids and mannosylerythritol lipids.

4. Cleaning fluid (11) for use in a closed circuit in a cleaning system (18, 31), comprising a concentrate (10) according to one of the preceding claims, and further comprising water for diluting the concentrate, wherein the cleaning fluid (11) comprises 1-10 times the quantity of water in relation to the quantity of concentrate.

5. Cleaning fluid according to claim 4, characterised in that the concentrate (10) comprises anionic surfactants.

6. Cleaning fluid according to one of claims 4 or 5, characterised in that the membrane lipid is a glycolipid, which originates from the group of the following substances: a) rhamnolipids b) sophorolipids c) trehalose and other mycological acidic glycolipids d) cellobiose lipids and mannosylerythritol lipids.

7. Method for providing a cleaning fluid (11) circulating in a cleaning system (18, 31) for workpieces (33a, 33b, 33c), comprising the following steps: a) providing a concentrate (10) according to one of claims 1 to 3 of a cleaning fluid (11), which comprises at least two different phases (12, 13), self-separating from each other, wherein the first phase (12) comprises a solution with bacterial spores, b) diluting the concentrate (10) with water to form a cleaning fluid (11), wherein step b) is carried out directly before or during application of the concentrate (10) into a cleaning system (18, 31).

8. Method according to claim 7, characterised in that the concentrate (10) or the cleaning fluid (11) comprises anionic surfactants.

9. Method for cleaning, in particular residue-free cleaning, of workpieces (33), the surfaces of which have been afflicted with oil or lubricant contamination, comprising the following steps: a) providing a cleaning fluid (11) according to one of claims 4 to 6, which comprises a solution having bacterial spores, and which has at least one membrane lipid, in particular a glycolipid, b) circulating the cleaning solution, c) heating the cleaning solution, d) supplying air and / or oxygen to the cleaning solution.

10. Container (15) having a concentrate (10) according to one of claims 1 to 3, for removal, in particular residue-free removal, of oil or lubricant contamination on surfaces of workpieces (33), wherein the concentrate comprises two different phases (12,13) self-separating from each other, wherein the first phase (12) comprises a solution having bacteria spores, and wherein the second phase (13) comprises a mixture, which comprises at least the following components: i) non-ionic surfactants ii) hydrotropes (clarifying agents) iii) complexing agents iv) preservatives wherein the first phase and / or the second phase have at least one membrane lipid, in particular a glycolipid.

11. Use of a concentrate (10), according to one of claims 1 to 3, wherein the concentrate comprises two different phases (12, 13) self-separating from each other, wherein the first phase (12) comprises a solution having bacterial spores, and wherein the second phase (13) comprises a mixture, which comprises at least the following components: i) non-ionic surfactants ii) hydrotropes (clarifying agents) iii) complexing agents iv) preservatives wherein the first phase (12) and / or the second phase (13) have at least one membrane lipid, in particular a glycolipid, as a circulating cleaning fluid for in particular residue-free removal of oil or lubricant contamination on surfaces of workpieces (33).

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