Isothermal amplification of nucleic acids

Abstract

The present disclosure relates to the amplification of nucleic acids in a sample using isothermal application process, e.g., RPA. In particular, the present disclosure provides methods for amplifying at least one target nucleic acid in a sample, where the reagents used in the method include trehalose and polyethylene glycol (PEG). The present disclosure further provides compositions, systems and kits for performing such methods.

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