Promoter for the specific expression of genes in layer 5 cells in mouse cortex

EP4766839A1Pending Publication Date: 2026-07-01NOVARTIS FORSCHUNGSSTIFTUNG ZWEIGNIEDERLASSUNG FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH
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Patent Information

Authority / Receiving Office
EP · EP
Patent Type
Applications
Current Assignee / Owner
NOVARTIS FORSCHUNGSSTIFTUNG ZWEIGNIEDERLASSUNG FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH
Filing Date
2024-08-20
Publication Date
2026-07-01

AI Technical Summary

Technical Problem

There is a need for new nucleic acid sequences that can drive the specific expression of recombinant genes in mammalian cells, particularly in layer 5 cells of the mouse cortex, with high expression levels and cell-type specificity.

Method used

A fragment of the CHODL gene has been identified to serve as a promoter that specifically drives the expression of heterologous genes in layer 5 cells of the mouse cortex. The nucleic acid sequence of this promoter (SEQ ID NO:1) or sequences with at least 70% identity to it, when used in an expression cassette, ensures specific gene expression in these cells.

Benefits of technology

The use of the CHODL gene fragment as a promoter achieves high and specific expression of genes in layer 5 cells of the mouse cortex, addressing the need for cell-type-specific gene expression in mammalian cells.

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Abstract

The present invention provides an isolated nucleic acid molecule comprising, or consisting of, the nucleic acid sequence of SEQ ID NO:1 or of a nucleic acid sequence of at least 1400 bp having at least 80% identity to said sequence of SEQ ID NO:1, wherein said isolated nucleic acid molecule leads to the specific expression of an exogenous gene in layer 5 cells in mouse cortex when a nucleic acid sequence coding for said exogenous gene is operatively linked to said isolated nucleic acid molecule.
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