Microbial compositions and methods
Patent Information
- Application Number
- EP2024820863
- Authority / Receiving Office
- EP · EP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-07-22
- Filing Date
- 2024-11-04
- Publication Date
- 2026-09-09
AI Technical Summary
Existing microbial compositions and methods for enhancing plant growth and soil characteristics have limitations that need to be addressed for improved efficacy.
The development of microbial compositions comprising a consortium of microbes, including Streptomyces and other bacterial genera, which are specifically selected and combined to enhance plant germination, growth, and soil health. These microbial consortia are formulated to include specific strains with identified 16S rRNA gene sequences, ensuring optimal microbial interactions and activities.
The proposed microbial compositions demonstrate enhanced plant growth parameters such as increased biomass, improved soil water retention, and better soil structure, compared to untreated controls.
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Abstract
Description
Docket No. OATH-015-PCT2 MICROBIAL COMPOSITIONS AND METHODS CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims priority to U.S. Provisional Application No.63 / 547,326, filed November 3, 2023, U.S. Provisional Application No.63 / 599,944, filed November 16, 2023, U.S. Provisional Application No.63 / 674,021, filed July 22, 2024, and U.S. Provisional Application No.63 / 648,580, filed May 16, 2024, the contents of which are incorporated herein by reference in their entireties. SEQUENCE LISTING
[0002] The instant application contains a Sequence Listing which has been submitted electronically in .XML format and is hereby incorporated by reference in its entirety. Said .XML copy, created on October 29, 2024, is named OATH-015_PCT2_SL.xml and is 1,102,911 bytes in size. The sequence listing contained in this .XML file is part of the specification and is hereby incorporated by reference herein in its entirety. BACKGROUND
[0003] Many microbes having beneficial effects on plant growth and properties are known; in addition these microbes may have beneficial effects on soil characteristics. Although compositions and methods utilizing such microbes have been described, there remains a need for improvement. INCORPORATION BY REFERENCE
[0004] All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference. BRIEF DESCRIPTION OF THE DRAWINGS
[0005] The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:
[0006] Figure 1a shows a method of preparation of a microbial composition from individual microbial stocks.
[0007] Figure 1b shows a method of growth and treatment of a microbial stock.Docket No. OATH-015-PCT2
[0008] Figure 1c shows a method of preparation of a microbial composition.
[0009] Figure 1d shows a method of combining one or more microbes and one or more heterologous components.
[0010] Figure 2 shows the topography of a Maximum-Likelihood phylogenetic tree displaying 195 ribotype gene signatures representing the OSL probiotic culture collection.
[0011] Figure 3 shows water holding capacity of soil samples.
[0012] Figure 4 shows turf resulting from treated (right) and untreated (left) seeds.
[0013] Figure 5 shows a box and whiskers chart of increased biomass in treated plants.
[0014] Figure 6 shows common bean root biomass, in plants growing in soil treated with a microbial consortium and untreated.
[0015] Figure 7 shows common bean shoot biomass, in plants growing in soil treated with a microbial consortium and untreated.
[0016] Figure 8 shows common bean pod biomass, in plants growing in soil treated with a microbial consortium and untreated.
[0017] Figure 9 shows grain biomass, in plants growing in soil treated with a microbial consortium and untreated.
[0018] Figure 10 shows soil water content in soil in which almond trees were grown, soil treated with a microbial consortium and untreated.
[0019] Figure 11 shows stem water potential in almond trees growing in soil treated with a microbial consortium and untreated.
[0020] Figure 12 shows soil bulk density in soil in which almond trees were grown, soil treated with a microbial consortium and untreated.
[0021] Figure 13 shows streaking pattern of agar plates streaked for purity determination.
[0022] Figure 14 shows landscape inoculation of plates for solid state fermentation of microbes. DETAILED DESCRIPTION I. Compositions and methods A. Microbes
[0023] Provided herein are compositions and methods including and / or utilizing one or more microbes, or that result from utilizing one or more microbes, in plant germination, growth, maintenance, and / or use, and other uses as described herein. The one or more microbes may be used in conjunction with any suitable plant or plants and the soil or other growth medium in which the plant or plants grow. As used herein, "microbe" or "microorganism" are usedDocket No. OATH-015-PCT2 interchangeably and include the two prokaryotic domains, Bacteria and Archaea, as well as eukaryotic fungi and protists. When a specific strain, deposit, or the like, of a microbe is described, this includes progeny and / or mutants thereof. The terms “plurality of microbes,” “plurality of microorganisms,” “consortium of microbes,” “consortium of microorganisms,” are used interchangeably herein, and include groups of two or more different microbes. Unless indicated otherwise, and as used herein, the term “different microbe,” includes a microbe that is taxonomically different from another microbe, e.g., different class, order, family, genus, species, or strain. Unless indicated otherwise, and as used herein, the term “different microbes,” includes two or more microbes, each of which is taxonomically different from the other microbes, e.g., different class, order, family, genus, species, or strain.
[0024] The term "plant," as used herein, includes a whole plant, any reproductive or developmental form or stage thereof, or to a part or fragment of a plant. The term "plant" may therefore be used to encompass plant propagules, seedlings, germinants, tube stock and mature plants or any parts or fragments thereof, including but not limited to hypocotyls, leaves, branches, stems, roots, heads / kernels, crown, tissue samples, seeds, fruits, nuts, flowers or cones.
[0025] Any suitable microbe can be used, such as a bacteria, an archaea, a protozoa, an algae, a fungus, for example a mycorrhizae, a virus, or a combination thereof. Preferably, the microbe is, at some point in its lifecycle, a unicellular organism; however, the microbe can exist in a multicellular state. In preferred embodiments, the compositions and / or methods comprise one or more bacteria. In certain embodiments, the compositions and / or methods comprise one or more fungi. In certain embodiments, the compositions and / or methods comprise one or more bacteria and one or more fungi.
[0026] In certain embodiments, the one or more microbes are bacteria. In certain embodiments, the one or more microbes are rhizobacteria. In certain embodiments, the one or more microbes are saprophytic. In certain embodiments, the one or more microbes include microbes belonging to the genus of Streptomyces. The microbe can be any suitable species of Streptomyces, including but not limited to Streptomyces achromogenes, Streptomyces albogriseolus, Streptomyces antibioticus, Streptomyces bacillari, Streptomyces bikiniensis, Streptomyces bobili, Streptomyces chartreusis, Streptomyces echinatu, Streptomyces echinatus, Streptomyces eurythermus, Streptomyces globosus, Streptomyces griseoaurantiacus, Streptomyces griseofuscus, Streptomyces griseoincarnatus, Streptomyces griseoruber, Streptomyces griseoviridis, Streptomyces kurssanovii, Streptomyces misionensis, Streptomyces murinus, Streptomyces musisoli, Streptomyces nigra, Streptomyces nigrescens, Streptomyces olivaceus, Streptomyces rochei, Streptomyces sennicomposti, Streptomyces sp000721605, Streptomyces sp019049245, and / or Streptomyces toxytricini, Streptomyces achromogenes,Docket No. OATH-015-PCT2 Streptomyces albogriseolus, Streptomyces antibioticus, Streptomyces bacillari, Streptomyces bikiniensis, Streptomyces bobili, Streptomyces chartreusis, Streptomyces echinatu, Streptomyces echinatus, Streptomyces eurythermus, Streptomyces globosus, Streptomyces griseoaurantiacus, Streptomyces griseofuscus, Streptomyces griseoincarnatus, Streptomyces griseoruber, Streptomyces griseoviridis, Streptomyces kurssanovii, Streptomyces misionensis, Streptomyces murinus, Streptomyces musisoli, Streptomyces nigra, Streptomyces nigrescens, Streptomyces olivaceus, Streptomyces rochei, Streptomyces sennicomposti, Streptomyces sp000721605, Streptomyces sp019049245, and / or Streptomyces toxytricini. In many cases, a species may be represented by more than one different strain; see Table 2A for strains of the above species, and SEQ ID NOs for 16S rRNA sequence or sequences associated with each strain, as well as ATCC deposit number in which the strain may be found. In some embodiments, a microbial composition of the present disclosure includes some or all of the microbes in the microbial consortia deposited with the American Type Culture Collection (ATCC) on October 30, 2024 and assigned deposit numbers PTA127834-127850. As used herein, a “16S rRNA sequence” or the like includes a RNA sequence for 16S rRNA or a nucleotide gene sequence for the 16S rRNA, as will be clear from context.
[0027] In certain embodiments, provided are compositions comprising microbes of one or more non-Streptomyces genera. In certain embodiments, provides are compositions comprising microbes of one or more species and / or strains of Streptomyces and microbes of one or more microbes of non-Streptomyces genera. In certain embodiments, the one or more non- Streptomyces genera comprise one or more of Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces, Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola_A, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, or Zhihengliuella or a combination thereof. In certain embodiments, provided are compositions comprising microbes of one or more species and / or strains of Streptomyces and microbes of the taxonomic class Bacilli, such as microbes of one or more genera including Paenibacillus, Lysinibacillis, Aneurinibacillus, Bacillus, Brevibacillus, Peribacillus, and / or Pristimantibacillus. In many cases, a species may be represented by more than one different strain; see Table 2A for strains of the above species, and SEQ ID NOs for 16S rRNA sequence or sequences associated with each strain, as well as ATCC deposit number in which the strain may be found. In some embodiments, a microbial composition of the present disclosure includes some or all of the microbes in the microbial consortia deposited with the American Type Culture Collection (ATCC) on October 30, 2024 and assigned deposit numbers PTA127834-127850.Docket No. OATH-015-PCT2
[0028] In certain embodiments, one or more microbes comprising one or more genetic sequences for 16S rRNA(s) are used. Sequences are as shown in Table 1 and Table 2A. Table 116S rRNA genetic sequencesDocket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2
[0029] In certain embodiments provided herein, a composition or method utilizes one or more microbes. Any suitable microbes may be used.
[0030] Certain of the microbes described in this Application were deposited with the American Type Culture Collection (ATCC, Manassas, Va.) on November 15, 2022, and October 30, 2024, and assigned the deposit numbers shown in Table 2 and Table 2A, respectively. The deposits were made under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microbes for the Purposes of Patent Procedure. Table 2: ATCC depositsDocket No. OATH-015-PCT2Docket No. OATH-015-PCT2Table 2A 16s rRNA Genetic Sequences and ATCC deposit numbers for selected microbesDocket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2
[0031] As shown in Example 32, the original 140 stocks (ATCC deposit #s PTA-127367 through PTA-127413 as shown in Table 2 and Oath-maintained deposits O-46 through O-140) were sequenced using PacBio full length 16S RNA gene sequencing. Once the results were readyDocket No. OATH-015-PCT2 16S sequences were identified using GenBank’s data base and sequence homology searches. 99% sequence identity was used as the threshold value to add a species name to each 16S gene. The outcome of this analyses showed that multiple of the original 140 stocks comprised more than one microbial species. A new set of internal stocks was developed comprising single microbial isolates. Literature and conventional microbiology knowledge were used to select correct phenotypes for further sequencing evaluation. If there were any unknown phenotypes they were also sent for 16S sequencing. The effort resulted in the production of the isolates shown in Table 32 (Example 32), reproduced below. Table 32 shows the updated isolate (ID# and taxonomic prediction) information as well as its corresponding designation from Table 1 (Strain # and Genus / Species). The matches are represented with BLAST comparisons of the 16S rRNA sequences. BLAST values (% identity and alignment length) are shown under the alignment info. Using Tables 1, 2, 2A, and 32, deposit numbers, 16S rRNA genetic sequences, and genus and species designations of the bacteria deposited with ATCC on November 15, 2022 may be matched with those deposited on October 30, 2024. Table 32: isolate correlation matrixDocket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2Docket No. OATH-015-PCT2
[0032] Deposits of each of O-46 through O140 is and has been maintained by Oath, Inc., or its predecessors or agents, at either 568 Duncan Farms Rd., Steele AL 35987, 4136 E Bujia Segunda, Tucson, AZ, 85718 or 505 Coastal South Blvd., La Jolla, CA.92307, since prior to the filing date of this application. Access to these deposits will be available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request, as well as to equivalent bodies and persons outside the United States. Upon allowance of any claims in the application, the Applicant(s) will make available to the public without restriction deposits of each of O-46 through O140 with the American Type Culture Collection (ATCC, Manassas, Va.) or other depository that conforms to the Budapest Treaty. The bacteria deposited with the ATCC will be taken from the same deposits maintained at the locations described above. Additionally, Applicant will meet all the requirements of 37 C.F.R. § 1.801-1.809, including providing an indication of the viability of the sample when the deposit is made.
[0033] This deposit of the aforementioned bacteria will be maintained in the ATCC or other depository that conforms to the Budapest Treaty, which are public depositories, for a period of 30 years, or 5 years after the most recent request, or for the enforceable life of the patent, whichever is longer, and will be replaced if it ever becomes nonviable during that period. Applicant will impose no restrictions on the availability of the deposited material; however,Docket No. OATH-015-PCT2 Applicant has no authority to waive any restrictions imposed by law on the transfer of biological material or its transportation in commerce.
[0034] In certain embodiments, fungi are used, e.g., separately, or in addition to the Streptomyces and / or other bacteria. The term “mycorrhiza” or “mycorrhizal fungus or fungi,” as used herein includes a fungal cell associated with a root of a plant. The mycorrhizal fungus can be an endomycorrhizal fungus or an ectomycorrhizal fungus. The mycorrhizal fungus can be an Arbuscular endomycorrhizal fungus, Ericoid endomycorrhizal fungus, Arbutoid endomycorrhizal fungus, Monotropoid endomycorrhizal fungus, orchidaceous endomycorrhizal fungus, or ectendomycorrhizal fungus, or other suitable fungus. In certain embodiments, the mycorrhizal fungus comprises any one of the mycorrhizal fungi identified in the FungalRoot database (Soudzilovskaia et al. (2020) NEW PHYTOLOGIST; <https: / / www.gbif.org / dataset / 744edc21-8dd2- 474e-8a0b-b8c3d56a3c2d>). In certain embodiments endomycorrhizal fungi belonging to the phylum Glomeromycota are used. In embodiments disclosed herein, any suitable fungi may be used. Certain embodiments comprise fungi of one or more of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, Sebacina, Alternaria, Beauveria, Cladiosporium, Claroideoglomus, Funneliformis, Glomus, Laccaria, Lecanicillium, Metarhizium, Piriformospora, Pisolithus, Rhizophagus, Serendipita, Talaromyces, Trichoderma, Mucor, and / or Phanerochaete genera. Certain embodiments comprise fungi of one or more of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, Sebacina, Alternaria, Beauveria, Cladiosporium, Claroideoglomus, Funneliformis, Glomus, Laccaria, Lecanicillium, Metarhizium, Piriformospora, Pisolithus, Rhizophagus, Serendipita, Talaromyces, and / or Trichoderma genera. B. Compositions comprising one or more microbes 1. Microbial compositions
[0035] Provided herein are microbial compositions.
[0036] In certain embodiments provided is a composition comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, or 60 and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, 60, 70, 80, or 100, preferably 2-100, more preferably 5-100, even more preferably 5-80, still more preferably 5-40, yet more preferably 10-80, still yet more preferably 10-70, even more preferably 10-60, even still more preferably 10-40, further preferably 10-30, different strains of Streptomyces microbes. Any suitable microbe microbes, such as those described herein, such as those described in Table 2A, may be used. In certain embodiments the composition further comprisesDocket No. OATH-015-PCT2 cells of one or more non-Streptomyces microbes. Any suitable non-Streptomyces microbes, such as those described herein, may be used. In certain embodiments, two or more of the microbes are heterologous to each other. In certain embodiments, the microbes of one or more non- Streptomyces microbe comprises microbes of one or more genera comprising Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces, Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola_A, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, and / or Zhihengliuella. In certain embodiments, the in one or more non-Streptomyces microbes comprise one or more Pseudomonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microvirga microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Paenibacillus microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Rhodococcus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Lysinibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Actinoplanes microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Agromyces microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Allosphingosinicella microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Aneurinibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Arthrobacter microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Bacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Brevibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Caldimonas microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Cupriavidus microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Erythrobacter microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Kocuria microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microbacterium microbes; additionallyDocket No. OATH-015-PCT2 or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microbispora microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Mycetocola microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Neorhizobium microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Nocardia microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Nocardioides microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Nonomuraea microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Peribacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Priestia microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Pristimantibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Stenotrophomonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Stutzerimonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Terrimonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Zhihengliuella microbes. In certain embodiments the composition comprises one or more of the microbes of Table 2A. In certain embodiments, the one or more microbes, e.g., Streptomyces microbes, comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 68- 109, 120-198, 202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437. In certain embodiments the one or more microbes, e.g., Streptomyces microbes, comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 197, 198, 202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-Docket No. OATH-015-PCT2 392, 394, 398, 403, 414, 423, 425-431, and / or 437. In certain embodiments, each microbe is present in a concentration of at least 105, 106, 107, 108, 109, or 1010CFU / gm and / or not more than 106, 107, 108, 109, 1010or 1011CFU / gm, preferably 105to 1010CFU / gm, more preferably 106to 1010CFU / gm, yet more preferably 107to 1010CFU / gm, even more preferably 106to 1010CFU / gm.
[0037] In certain embodiments, provided herein is a composition comprising one or more Streptomyces microbes, wherein the one or more Streptomyces microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence to one or more of SEQ ID NOs:68-109, 120-198, 202- 209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437. In certain embodiments, the one or more Streptomyces microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence to one or more of SEQ ID NOs:197, 198, 202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306- 322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437. In certain embodiments the composition comprises microbes of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, or 60 and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, 60, 70, 80, or 100, preferably 2-100, more preferably 5-80, even more preferably 5-40, still more preferably 5-30, yet more preferably 10-80, still yet more preferably 10-70, even more preferably 10-60, yet even more preferably 10-30 different Streptomyces strains. In certain embodiments, each microbe is present in a concentration of at least 105, 106, 107, 108, 109, or 1010CFU / gm and / or not more than 106, 107, 108, 109, 1010or 1011CFU / gm, preferably 105to 1010CFU / gm, more preferably 106to 1010CFU / gm, yet more preferably 107to 1010CFU / gm, even more preferably 106to 1010CFU / gm. In certain embodiments the composition further comprises cells of one or more non-Streptomyces microbes. Any suitable non-Streptomyces microbes, such as those described herein, may be used. In certain embodiments, two or more of the microbes are heterologous to each other. In certain embodiments, the microbes of one or more non- Streptomyces microbe comprises microbes of one or more genera comprising Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces,Docket No. OATH-015-PCT2 Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola_A, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, and / or Zhihengliuella. In certain embodiments, the in one or more non-Streptomyces microbes comprise one or more Pseudomonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microvirga microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Paenibacillus microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Rhodococcus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Lysinibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Actinoplanes microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Agromyces microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Allosphingosinicella microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Aneurinibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Arthrobacter microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Bacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Brevibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Caldimonas microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Cupriavidus microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Erythrobacter microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Kocuria microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microbacterium microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microbispora microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Mycetocola microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Neorhizobium microbes; additionally or alternatively, in certainDocket No. OATH-015-PCT2 embodiments the one or more non-Streptomyces microbes comprise one or more Nocardia microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Nocardioides microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Nonomuraea microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Peribacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Priestia microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Pristimantibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Stenotrophomonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Stutzerimonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Terrimonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Zhihengliuella microbes. In certain embodiments, the composition comprises one or more of the microbes of Table 2A One or more of the microbes may be heterologous to one or more other microbes.
[0038] In certain embodiments, provided herein is a composition comprising Streptomyces spp. microbes and cells of at least one, two, three, four, five, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 20, 22, 25, 27, 30, or 32 and / or not more than one, two, three, four, five, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 20, 22, 25, 27, 30, 32, or 35 preferably 2-35, more preferably 3-35, yet more preferably 5-35, even yet more preferably 5-20, still more preferably 5-15, such as 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 additional microbial species selected from the group consisting of Pseudomonas spp., Microvirga spp., Paenibacillus spp., Rhodococcus spp., Lysinibacillis spp., Actinoplanes spp., Agromyces spp., Allosphingosinicella spp., Aneurinibacillus spp., Arthrobacter spp., Bacillus spp., , Brevibacillus spp., Caldimonas spp., Cupriavidus spp., Erythrobacter spp., Kocuria spp., Microbacterium spp., Microbispora spp., Mycetocola spp., Neorhizobium spp., Nocardia spp., Nocardioides spp., Nonomuraea spp., Peribacillus spp., Priestia spp., Pristimantibacillus spp., Stenotrophomonas spp., Stutzerimonas spp., Terrimonas spp., and Zhihengliuella spp. In certain embodiments, the composition comprises microbes of at least 2, 3, 5, 7, 10, 12, 15, 20, 30, 40, or 50 and / or not more than 3, 5, 7, 10, 12, 15, 20, 30, 40, 50, 60, 80, or 100 different Streptomyces strains, for example 2-100 different Streptomyces strains, or 2-40, or 2-30. In certain embodiments, the in one or more non-Streptomyces microbes comprise one or more Pseudomonas microbes, such as at least 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, 16 or 18 and / or not moreDocket No. OATH-015-PCT2 than 3, 4, 5, 6, 7, 8, 10, 12, 14, 16, 18, 20 or 24 different Pseudomonas strains, for example 2-24 different Pseudomonas strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microvirga microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Paenibacillus microbes, such as at least 2, 3, 4, 5, 6, or 7 and / or not more than 3, 4, 5, 6, 7, 8, or 10 different Paenibacillus species, for example 2-10 different Paenibacillus strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Rhodococcus microbes, such as at least 1, or at least 2 different Rhodococcus strains; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Lysinibacillus microbes, such as at least 1, 2, 3, 4, 5, 6, or 7 and / or not more than 3, 4, 5, 6, 7, 8, or 10 different Lysinibacillus strains, for example 1-10, preferably 1-5, more preferably 1, or 2, different Lysinibacillus strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Actinoplanes microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Agromyces microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Allosphingosinicella microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Aneurinibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Arthrobacter microbes, such as at least 2, 3, 4, 5, 6, or 7 and / or not more than 3, 4, 5, 6, 7, 8, or 10 different Arthrobacter strains, for example 2-10 different Arthrobacter strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Bacillus microbes, such as at least 1, 2, 3, 4, 5, 6, or 7 and / or not more than 2, 3, 4, 5, 6, 7, 8, or 10 different Bacillus strains, for example 1-10, preferably 2-10 different Bacillus strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Brevibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Caldimonas microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Cupriavidus microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Erythrobacter microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Kocuria microbes ; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microbacterium microbes, such as at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6 different Microbacterium strains, for example 1-6, preferably 1-4, different MicrobacteriumDocket No. OATH-015-PCT2 strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Microbispora microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Mycetocola microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Neorhizobium microbes, such as microbes of 1, or microbes of 2, different Neorhizobium strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Nocardia microbes, such as microbes of 1, or microbes of 2, different Nocardia strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Nocardioides microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Nonomuraea microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Peribacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Priestia microbes microbes of 1, or microbes of 2, different Priestia strains; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Pristimantibacillus microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Stenotrophomonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Stutzerimonas microbes; additionally or alternatively, in certain embodiments the one or more non- Streptomyces microbes comprise one or more Terrimonas microbes; additionally or alternatively, in certain embodiments the one or more non-Streptomyces microbes comprise one or more Zhihengliuella microbes. In certain embodiments, the composition comprises one or more of the microbes of Table 2A. In certain embodiments, the one or more microbes, e.g., Streptomyces microbes, comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 68-109, 120-198, 202-209, 211-220, 222-246, 248-250, 252-256, 258- 278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375- 377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437. In certain embodiments, the one or more microbes, e.g., Streptomyces microbes, comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleicDocket No. OATH-015-PCT2 acid sequence of one or more of SEQ ID NOs: 197, 198202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.
[0039] In certain embodiments, compositions provided herein comprise nitrogen-fixing bacteria. In certain embodiments, provided is a composition comprising microbes of one or more Streptomyces species and microbes of at least one non-Streptomyces species, wherein the non- Streptomyces species is a nitrogen-fixing species. In certain embodiments the nitrogen-fixing non-Streptomyces species comprise microbes of one or more of Pseudomonas spp., Microvirga spp., Bradyrhyizobium spp., Aureimonas spp., Paenibacillus spp., and / or Lysinibacillus spp. In certain embodiments the nitrogen-fixing non-Streptomyces species comprise microbes of one or more of Cupriavidus, Neorhizobium, Paenibacillus, Pseudomonas, Bacillus, Arthrobacter, and / or Lysinibacillus. In certain embodiments the composition comprises microbes of Pseudomonas spp. In certain embodiments the composition comprises microbes of Microvirga spp. In certain embodiments the composition comprises microbes of Aureimonas spp. In certain embodiments the composition comprises microbes of Paenibacillus spp. In certain embodiments the composition comprises microbes of Bradyrhizobium spp. In certain embodiments the composition comprises microbes of Lysinibacillis spp. In certain embodiments the composition comprises microbes of Bacillus spp. In certain embodiments the composition comprises microbes of Brevibacillus spp. In certain embodiments the composition comprises microbes of Aneurinibacillus spp.
[0040] In certain embodiments provided herein is a composition comprising one or more microbes of ATCC deposits designated 1-45, deposited as ATCC Nos. PTA-127367-127413, respectively, as shown in Table 2 and / or one or more microbes of OATH-46-OATH-140, and / or one or more microbes of one or more of ATCC deposits PTA-127834-127850.
[0041] In embodiments including one or more Streptomyces microbes, any suitable Streptomyces microbes may be used. The microbes may be of one or more Streptomyces species and / or one or more Streptomyces strains. In certain embodiments, microbes are microbes of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, or 60, for example at least 5, or at least 10, or at least 15, or at least 20, and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, 60, 70, 80, or 100, preferably 2-100, more preferably 5-100, even more preferably 5-80, still more preferably 5-40, yet more preferably 10-80, still yet more preferably 10-70, even more preferably 10-60 yet even more preferably 10-30 different Streptomyces strains.
[0042] In embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces achromogenes, such as microbes of one or moreDocket No. OATH-015-PCT2 Streptomyces achromogenes strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 15 Streptomyces achromogenes strains, such as 1-15, preferably 1-12, more preferably 1-10, even more preferably 2-15, still more preferably 2-12. In certain embodiments, one or more Streptomyces achromogenes microbes comprise a first microbe of a first Streptomyces achromogenes strain. In certain embodiments, one or more Streptomyces achromogenes microbes comprise a second microbe of a second Streptomyces achromogenes strain, different from the first strain. In certain embodiments, one or more Streptomyces achromogenes microbes comprise a third microbe of a third Streptomyces achromogenes strain, different from the first and second strains. In certain embodiments, one or more Streptomyces achromogenes microbes comprise a fourth microbe of a fourth Streptomyces achromogenes strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces achromogenes strain, such as the strain designated OATH00343, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces achromogenes strain, such as the strain designated OATH00601 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces achromogenes strain, such as the strain designated OATH00701, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces achromogenes strain, such as the strain designated OATH00702 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces achromogenes strain, such as the strain designated OATH01021, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces achromogenes strain, such as the strain designated OATH01022 are used. Alternatively or additionally, in certain embodiments, microbes of a seventh Streptomyces achromogenes strain, such as the strain designated OATH01071 are used. Alternatively or additionally, in certain embodiments, microbes of an eighth Streptomyces achromogenes strain, such as the strain designated OATH01242 are used. Alternatively or additionally, in certain embodiments, microbes of a ninth Streptomyces achromogenes strain, such as the strain designated OATH01244 are used. Alternatively or additionally, in certain embodiments, microbes of a tenth Streptomyces achromogenes strain, such as the strain designated OATH01261 are used. Alternatively or additionally, in certain embodiments, microbes of an eleventh Streptomyces achromogenes strain, such as the strain designated OATH01262 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces achromogenes strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferablyDocket No. OATH-015-PCT2 at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A.
[0043] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces albogriseolus, such as microbes of one or more Streptomyces albogriseolus strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces albogriseolus strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces albogriseolus microbes comprise a first microbe of a first Streptomyces albogriseolus strain. In certain embodiments, one or more Streptomyces albogriseolus microbes comprise a second microbe of a second Streptomyces albogriseolus strain, different from the first strain. In certain embodiments, one or more Streptomyces albogriseolus microbes comprise a third microbe of a third Streptomyces albogriseolus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces albogriseolus microbes comprise a fourth microbe of a fourth Streptomyces albogriseolus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces albogriseolus strain, such as the strain designated OATH01851, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces albogriseolus strain, such as the strain designated OATH02031 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces albogriseolus strain, such as the strain designated OATH02041, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces albogriseolus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0044] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces antibioticus, such as microbes of one or more Streptomyces antibioticus strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, or 8 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, or 12 Streptomyces antibioticus strains, such as 1- 12, preferably 1-10, more preferably 1-8, even more preferably 2-12, still more preferably 2-10. In certain embodiments, one or more Streptomyces antibioticus microbes comprise a first microbe of a first Streptomyces antibioticus strain. In certain embodiments, one or more Streptomyces antibioticus microbes comprise a second microbe of a second Streptomyces antibioticus strain, different from the first strain. In certain embodiments, one or moreDocket No. OATH-015-PCT2 Streptomyces antibioticus microbes comprise a third microbe of a third Streptomyces antibioticus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces antibioticus microbes comprise a fourth microbe of a fourth Streptomyces antibioticus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces antibioticus strain, such as the strain designated OATH00354, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces antibioticus strain, such as the strain designated OATH00371 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces antibioticus strain, such as the strain designated OATH00372, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces antibioticus strain, such as the strain designated OATH00373 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces antibioticus strain, such as the strain designated OATH00374, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces antibioticus strain, such as the strain designated OATH00375 are used. Alternatively or additionally, in certain embodiments, microbes of a seventh Streptomyces antibioticus strain, such as the strain OATH00376 designated are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces antibioticus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0045] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces bacillari, such as microbes of one or more Streptomyces bacillari strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces bacillari strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces bacillari microbes comprise a first microbe of a first Streptomyces bacillari strain. In certain embodiments, one or more Streptomyces bacillari microbes comprise a second microbe of a second Streptomyces bacillari strain, different from the first strain. In certain embodiments, one or more Streptomyces bacillari microbes comprise a third microbe of a third Streptomyces bacillari strain, different from the first and second strains. In certain embodiments, one or more Streptomyces bacillari microbes comprise a fourth microbe of a fourth Streptomyces bacillari strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces bacillari strain, such as the strainDocket No. OATH-015-PCT2 designated OATH00312, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces bacillari strain, such as the strain designated OATH00313 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces bacillari strain, such as the strain designated OATH00392, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces bacillari strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0046] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces bikiniensis, such as microbes of one or more Streptomyces bikiniensis strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces bikiniensis strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces bikiniensis microbes comprise a first microbe of a first Streptomyces bikiniensis strain. In certain embodiments, one or more Streptomyces bikiniensis microbes comprise a second microbe of a second Streptomyces bikiniensis strain, different from the first strain. In certain embodiments, one or more Streptomyces bikiniensis microbes comprise a third microbe of a third Streptomyces bikiniensis strain, different from the first and second strains. In certain embodiments, one or more Streptomyces bikiniensis microbes comprise a fourth microbe of a fourth Streptomyces bikiniensis strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces bikiniensis strain, such as the strain designated OATH00041, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces bikiniensis strain, such as the strain designated OATH00091 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces bikiniensis strain, such as the strain designated OATH01401, are used.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces bikiniensis strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.Docket No. OATH-015-PCT2
[0047] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces echinatus, such as microbes of one or more Streptomyces echinatus strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, or 8 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, or 12 Streptomyces echinatus strains, such as 1-12, preferably 1-10, more preferably 1-8, even more preferably 2-12, still more preferably 2-10. In certain embodiments, one or more Streptomyces echinatus microbes comprise a first microbe of a first Streptomyces echinatus strain. In certain embodiments, one or more Streptomyces echinatus microbes comprise a second microbe of a second Streptomyces echinatus strain, different from the first strain. In certain embodiments, one or more Streptomyces echinatus microbes comprise a third microbe of a third Streptomyces echinatus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces echinatus microbes comprise a fourth microbe of a fourth Streptomyces echinatus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces echinatus strain, such as the strain designated OATH00021, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces echinatus strain, such as the strain designated OATH00322 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces echinatus strain, such as the strain designated OATH00323, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces echinatus strain, such as the strain designated OATH00522 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces echinatus strain, such as the strain designated OATH00663, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces echinatus strain, such as the strain designated OATH00851 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces echinatus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0048] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces bobili, such as microbes of one or more Streptomyces bobili strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces bobili strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces bobili microbes comprise a first microbe of a first Streptomyces bobili strain. In certain embodiments, one or more Streptomyces bobili microbesDocket No. OATH-015-PCT2 comprise a second microbe of a second Streptomyces bobili strain, different from the first strain. In certain embodiments, one or more Streptomyces bobili microbes comprise a third microbe of a third Streptomyces bobili strain, different from the first and second strains. In certain embodiments, microbes of a first Streptomyces bobili strain, such as the strain designated OATH02051, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces bobili strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0049] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces chartreusis, such as microbes of one or more Streptomyces chartreusis strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces chartreusis strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces chartreusis microbes comprise a first microbe of a first Streptomyces chartreusis strain. In certain embodiments, one or more Streptomyces chartreusis microbes comprise a second microbe of a second Streptomyces chartreusis strain, different from the first strain. In certain embodiments, one or more Streptomyces chartreusis microbes comprise a third microbe of a third Streptomyces chartreusis strain, different from the first and second strains. In certain embodiments, microbes of a first Streptomyces chartreusis strain, such as the strain designated OATH00661, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces chartreusis strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A..
[0050] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces eurythermus, such as microbes of one or more Streptomyces eurythermus strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces eurythermus strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces eurythermus microbes comprise a first microbe of a first Streptomyces eurythermus strain. In certain embodiments, oneDocket No. OATH-015-PCT2 or more Streptomyces eurythermus microbes comprise a second microbe of a second Streptomyces eurythermus strain, different from the first strain. In certain embodiments, one or more Streptomyces eurythermus microbes comprise a third microbe of a third Streptomyces eurythermus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces eurythermus microbes comprise a fourth microbe of a fourth Streptomyces eurythermus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces eurythermus strain, such as the strain designated OATH01121, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces eurythermus strain, such as the strain designated OATH01243 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces eurythermus strain, such as the strain designated OATH01246, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces eurythermus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0051] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces globosus, such as microbes of one or more Streptomyces globosus strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 22, or 25 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 22, 25, 27, or 30 Streptomyces globosus strains, such as 1-30, preferably 1-27, more preferably 1-25, even more preferably 2-30, still more preferably 2-27. In certain embodiments, one or more Streptomyces globosus microbes comprise a first microbe of a first Streptomyces globosus strain. In certain embodiments, one or more Streptomyces globosus microbes comprise a second microbe of a second Streptomyces globosus strain, different from the first strain. In certain embodiments, one or more Streptomyces globosus microbes comprise a third microbe of a third Streptomyces globosus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces globosus microbes comprise a fourth microbe of a fourth Streptomyces globosus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces globosus strain, such as the strain designated OATH00801, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces globosus strain, such as the strain designated OATH00802 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces globosus strain, such asDocket No. OATH-015-PCT2 the strain designated OATH00912, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces globosus strain, such as the strain designated OATH01271 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces globosus strain, such as the strain designated OATH01273, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces globosus strain, such as the strain designated OATH01283 are used. Alternatively or additionally, in certain embodiments, microbes of a seventh Streptomyces globosus strain, such as the strain OATH01285 designated are used. Alternatively or additionally, in certain embodiments, microbes of an eighth Streptomyces globosus strain, such as the strain designated OATH01292 are used. Alternatively or additionally, in certain embodiments, microbes of a ninth Streptomyces globosus strain, such as the strain designated OATH01293 are used. Alternatively or additionally, in certain embodiments, microbes of a tenth Streptomyces globosus strain, such as the strain designated OATH01294 are used. Alternatively or additionally, in certain embodiments, microbes of an eleventh Streptomyces globosus strain, such as the strain designated OATH01301 are used. Alternatively or additionally, in certain embodiments, microbes of a twelfth Streptomyces globosus strain, such as the strain designated OATH01302 are used. Alternatively or additionally, in certain embodiments, microbes of a thirteenth Streptomyces globosus strain, such as the strain designated OATH01303 are used. Alternatively or additionally, in certain embodiments, microbes of a fourteenth Streptomyces globosus strain, such as the strain designated OATH01304 are used. Alternatively or additionally, in certain embodiments, microbes of a fifteenth Streptomyces globosus strain, such as the strain designated OATH01305 are used. Alternatively or additionally, in certain embodiments, microbes of a sixteenth Streptomyces globosus strain, such as the strain designated OATH01306 are used. Alternatively or additionally, in certain embodiments, microbes of an seventeenth Streptomyces globosus strain, such as the strain designated OATH01311 are used. Alternatively or additionally, in certain embodiments, microbes of an eithteenth Streptomyces globosus strain, such as the strain designated OATH01312 are used. Alternatively or additionally, in certain embodiments, microbes of a nineteenth Streptomyces globosus strain, such as the strain designated OATH01313 are used. Alternatively or additionally, in certain embodiments, microbes of a twentieth Streptomyces globosus strain, such as the strain designated OATH01314 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-first Streptomyces globosus strain, such as the strain designated OATH01315 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-second Streptomyces globosus strain, such as the strain designated OATH01316 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-third Streptomyces globosus strain, such as the strainDocket No. OATH-015-PCT2 designated OATH01341 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-fourth Streptomyces globosus strain, such as the strain designated OATH01351 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-fifth Streptomyces globosus strain, such as the strain designated OATH01352 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-sixth Streptomyces globosus strain, such as the strain designated OATH02141 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces globosus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0052] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces misionensis, such as microbes of one or more Streptomyces misionensis strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 22, or 25 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 22, 25, 27, or 30 Streptomyces misionensis strains, such as 1-30, preferably 1-27, more preferably 1-25, even more preferably 2-30, still more preferably 2-27. In certain embodiments, one or more Streptomyces misionensis microbes comprise a first microbe of a first Streptomyces misionensis strain. In certain embodiments, one or more Streptomyces misionensis microbes comprise a second microbe of a second Streptomyces misionensis strain, different from the first strain. In certain embodiments, one or more Streptomyces misionensis microbes comprise a third microbe of a third Streptomyces misionensis strain, different from the first and second strains. In certain embodiments, one or more Streptomyces misionensis microbes comprise a fourth microbe of a fourth Streptomyces misionensis strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces misionensis strain, such as the strain designated OATH00223, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces misionensis strain, such as the strain designated OATH00252 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces misionensis strain, such as the strain designated OATH00253, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces misionensis strain, such as the strain designated OATH00331 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces misionensis strain, such as the strain designated OATH00382, are used. Alternatively or additionally, in certain embodiments, microbes of aDocket No. OATH-015-PCT2 sixth Streptomyces misionensis strain, such as the strain designated OATH00413 are used. Alternatively or additionally, in certain embodiments, microbes of a seventh Streptomyces misionensis strain, such as the strain OATH00421 designated are used. Alternatively or additionally, in certain embodiments, microbes of an eighth Streptomyces misionensis strain, such as the strain designated OATH00423 are used. Alternatively or additionally, in certain embodiments, microbes of a ninth Streptomyces misionensis strain, such as the strain designated OATH00424 are used. Alternatively or additionally, in certain embodiments, microbes of a tenth Streptomyces misionensis strain, such as the strain designated OATH00425 are used. Alternatively or additionally, in certain embodiments, microbes of an eleventh Streptomyces misionensis strain, such as the strain designated OATH00491 are used. Alternatively or additionally, in certain embodiments, microbes of a twelfth Streptomyces misionensis strain, such as the strain designated OATH00492 are used. Alternatively or additionally, in certain embodiments, microbes of a thirteenth Streptomyces misionensis strain, such as the strain designated OATH00571 are used. Alternatively or additionally, in certain embodiments, microbes of a fourteenth Streptomyces misionensis strain, such as the strain designated OATH00651 are used. Alternatively or additionally, in certain embodiments, microbes of a fifteenth Streptomyces misionensis strain, such as the strain designated OATH00741 are used. Alternatively or additionally, in certain embodiments, microbes of a sixteenth Streptomyces misionensis strain, such as the strain designated OATH00742 are used. Alternatively or additionally, in certain embodiments, microbes of an seventeenth Streptomyces misionensis strain, such as the strain designated OATH00743 are used. Alternatively or additionally, in certain embodiments, microbes of an eithteenth Streptomyces misionensis strain, such as the strain designated OATH00891 are used. Alternatively or additionally, in certain embodiments, microbes of a nineteenth Streptomyces misionensis strain, such as the strain designated OATH00961 are used. Alternatively or additionally, in certain embodiments, microbes of a twentieth Streptomyces misionensis strain, such as the strain designated OATH01002 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-first Streptomyces misionensis strain, such as the strain designated OATH01241 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-second Streptomyces misionensis strain, such as the strain designated OATH01247 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-third Streptomyces misionensis strain, such as the strain designated OATH01411 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-fourth Streptomyces misionensis strain, such as the strain designated OATH00422 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of StreptomycesDocket No. OATH-015-PCT2 misionensis strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0053] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces murinus, such as microbes of one or more Streptomyces murinus strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 15 Streptomyces murinus strains, such as 1-15, preferably 1-12, more preferably 1-10, even more preferably 2-15, still more preferably 2-12. In certain embodiments, one or more Streptomyces murinus microbes comprise a first microbe of a first Streptomyces murinus strain. In certain embodiments, one or more Streptomyces murinus microbes comprise a second microbe of a second Streptomyces murinus strain, different from the first strain. In certain embodiments, one or more Streptomyces murinus microbes comprise a third microbe of a third Streptomyces murinus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces murinus microbes comprise a fourth microbe of a fourth Streptomyces murinus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces murinus strain, such as the strain designated OATH00261, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces murinus strain, such as the strain designated OATH00262 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces murinus strain, such as the strain designated OATH00263, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces murinus strain, such as the strain designated OATH00264 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces murinus strain, such as the strain designated OATH00562, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces murinus strain, such as the strain designated OATH00563 are used. Alternatively or additionally, in certain embodiments, microbes of a seventh Streptomyces murinus strain, such as the strain OATH00564 designated are used. Alternatively or additionally, in certain embodiments, microbes of an eighth Streptomyces murinus strain, such as the strain designated OATH00823 are used. Alternatively or additionally, in certain embodiments, microbes of a ninth Streptomyces murinus strain, such as the strain designated OATH00881 are used. Alternatively or additionally, in certain embodiments, microbes of a tenth Streptomyces murinus strain, such as the strain designated OATH01251 are used. AlternativelyDocket No. OATH-015-PCT2 or additionally, in certain embodiments, microbes of an eleventh Streptomyces murinus strain, such as the strain designated OATH01253 are used. Alternatively or additionally, in certain embodiments, microbes of an twelfth Streptomyces murinus strain, such as the strain designated OATH01412 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces murinus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0054] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces musisoli, such as microbes of one or more Streptomyces musisoli strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces musisoli strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces musisoli microbes comprise a first microbe of a first Streptomyces musisoli strain. In certain embodiments, one or more Streptomyces musisoli microbes comprise a second microbe of a second Streptomyces musisoli strain, different from the first strain. In certain embodiments, one or more Streptomyces musisoli microbes comprise a third microbe of a third Streptomyces musisoli strain, different from the first and second strains. In certain embodiments, one or more Streptomyces musisoli microbes comprise a fourth microbe of a fourth Streptomyces musisoli strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces musisoli strain, such as the strain designated OATH00521, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces musisoli strain, such as the strain designated OATH00662 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces musisoli strain, such as the strain designated OATH00681, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces musisoli strain, such as the strain designated OATH01101, are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces musisoli strain, such as the strain designated OATH01102, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces musisoli strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably atDocket No. OATH-015-PCT2 least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0055] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces sennicomposti, such as microbes of one or more Streptomyces sennicomposti strains, e.g., at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 22, 25, 27, or 30 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 22, 25, 27, 30, or 35 Streptomyces sennicomposti strains, such as 1-35, preferably 1-32, more preferably 1-30, even more preferably 2-35, still more preferably 2-32. In certain embodiments, one or more Streptomyces sennicomposti microbes comprise a first microbe of a first Streptomyces sennicomposti strain. In certain embodiments, one or more Streptomyces sennicomposti microbes comprise a second microbe of a second Streptomyces sennicomposti strain, different from the first strain. In certain embodiments, one or more Streptomyces sennicomposti microbes comprise a third microbe of a third Streptomyces sennicomposti strain, different from the first and second strains. In certain embodiments, one or more Streptomyces sennicomposti microbes comprise a fourth microbe of a fourth Streptomyces sennicomposti strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces sennicomposti strain, such as the strain designated OATH00031, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces sennicomposti strain, such as the strain designated OATH00071 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces sennicomposti strain, such as the strain designated OATH00072, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces sennicomposti strain, such as the strain designated OATH00101 are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces sennicomposti strain, such as the strain designated OATH00103, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces sennicomposti strain, such as the strain designated OATH00241 are used. Alternatively or additionally, in certain embodiments, microbes of a seventh Streptomyces sennicomposti strain, such as the strain OATH00242 designated are used. Alternatively or additionally, in certain embodiments, microbes of an eighth Streptomyces sennicomposti strain, such as the strain designated OATH00243 are used. Alternatively or additionally, in certain embodiments, microbes of a ninth Streptomyces sennicomposti strain, such as the strain designated OATH00424 are used. Alternatively or additionally, in certain embodiments, microbes of a tenth Streptomyces sennicomposti strain, such as the strain designated OATH00301 are used. Alternatively or additionally, in certain embodiments, microbes of anDocket No. OATH-015-PCT2 eleventh Streptomyces sennicomposti strain, such as the strain designated OATH00381 are used. Alternatively or additionally, in certain embodiments, microbes of a twelfth Streptomyces sennicomposti strain, such as the strain designated OATH00383 are used. Alternatively or additionally, in certain embodiments, microbes of a thirteenth Streptomyces sennicomposti strain, such as the strain designated OATH00384 are used. Alternatively or additionally, in certain embodiments, microbes of a fourteenth Streptomyces sennicomposti strain, such as the strain designated OATH00402 are used. Alternatively or additionally, in certain embodiments, microbes of a fifteenth Streptomyces sennicomposti strain, such as the strain designated OATH00403 are used. Alternatively or additionally, in certain embodiments, microbes of a sixteenth Streptomyces sennicomposti strain, such as the strain designated OATH00404 are used. Alternatively or additionally, in certain embodiments, microbes of an seventeenth Streptomyces sennicomposti strain, such as the strain designated OATH00442 are used. Alternatively or additionally, in certain embodiments, microbes of an eithteenth Streptomyces sennicomposti strain, such as the strain designated OATH00443 are used. Alternatively or additionally, in certain embodiments, microbes of a nineteenth Streptomyces sennicomposti strain, such as the strain designated OATH00581 are used. Alternatively or additionally, in certain embodiments, microbes of a twentieth Streptomyces sennicomposti strain, such as the strain designated OATH00591 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-first Streptomyces sennicomposti strain, such as the strain designated OATH00592 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-second Streptomyces sennicomposti strain, such as the strain designated OATH00593 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-third Streptomyces sennicomposti strain, such as the strain designated OATH00641 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-fourth Streptomyces sennicomposti strain, such as the strain designated OATH00691 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-fifth Streptomyces sennicomposti strain, such as the strain designated OATH00692 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-sixth Streptomyces sennicomposti strain, such as the strain designated OATH00711 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-seventh Streptomyces sennicomposti strain, such as the strain designated OATH00753 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-eighth Streptomyces sennicomposti strain, such as the strain designated OATH00871 are used. Alternatively or additionally, in certain embodiments, microbes of a twenty-ninth Streptomyces sennicomposti strain, such as the strain designated OATH00901 are used. Alternatively or additionally, in certain embodiments, microbes of a thirtieth StreptomycesDocket No. OATH-015-PCT2 sennicomposti strain, such as the strain designated OATH01182 are used. Alternatively or additionally, in certain embodiments, microbes of a thirty-first Streptomyces sennicomposti strain, such as the strain designated OATH01183 are used. Alternatively or additionally, in certain embodiments, microbes of a thirty-second Streptomyces sennicomposti strain, such as the strain designated OATH01201 are used. Alternatively or additionally, in certain embodiments, microbes of a thirty-third Streptomyces sennicomposti strain, such as the strain designated OATH01331 are used.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces sennicomposti strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0056] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces rochei, such as microbes of one or more Streptomyces rochei strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces rochei strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces rochei microbes comprise a first microbe of a first Streptomyces rochei strain. In certain embodiments, one or more Streptomyces rochei microbes comprise a second microbe of a second Streptomyces rochei strain, different from the first strain. In certain embodiments, one or more Streptomyces rochei microbes comprise a third microbe of a third Streptomyces rochei strain, different from the first and second strains. In certain embodiments, one or more Streptomyces rochei microbes comprise a fourth microbe of a fourth Streptomyces rochei strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces rochei strain, such as the strain designated OATH00051, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces rochei strain, such as the strain designated OATH00052 are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces rochei strain, such as the strain designated OATH00053, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces rochei strain, such as the strain designated OATH0081, are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces rochei strain, such as the strain designated OATH00353, are used. Alternatively or additionally, in certain embodiments, microbes of a sixth Streptomyces rochei strain, such as the strain designated OATH00762, are used. 16S rRNA sequences andDocket No. OATH-015-PCT2 ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces rochei strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0057] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces griseoaurantiacus, such as microbes of one or more Streptomyces griseoaurantiacus strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces griseoaurantiacus strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces griseoaurantiacus microbes comprise a first microbe of a first Streptomyces griseoaurantiacus strain. In certain embodiments, one or more Streptomyces griseoaurantiacus microbes comprise a second microbe of a second Streptomyces griseoaurantiacus strain, different from the first strain. In certain embodiments, one or more Streptomyces griseoaurantiacus microbes comprise a third microbe of a third Streptomyces griseoaurantiacus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces griseoaurantiacus microbes comprise a fourth microbe of a fourth Streptomyces griseoaurantiacus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces griseoaurantiacus strain, such as the strain designated OATH00291, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces griseoaurantiacus strain, such as the strain designated OATH00292 are used.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces griseoaurantiacus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0058] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces griseoincarnatus, such as microbes of one or more Streptomyces griseoincarnatus strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces griseoincarnatus strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces griseoincarnatusDocket No. OATH-015-PCT2 microbes comprise a first microbe of a first Streptomyces griseoincarnatus strain. In certain embodiments, one or more Streptomyces griseoincarnatus microbes comprise a second microbe of a second Streptomyces griseoincarnatus strain, different from the first strain. In certain embodiments, one or more Streptomyces griseoincarnatus microbes comprise a third microbe of a third Streptomyces griseoincarnatus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces griseoincarnatus microbes comprise a fourth microbe of a fourth Streptomyces griseoincarnatus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces griseoincarnatus strain, such as the strain designated OATH00144 are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces griseoincarnatus strain, such as the strain designated OATH00321, are used. Alternatively or additionally, in certain embodiments, microbes of a third Streptomyces griseoincarnatus strain, such as the strain designated OATH00412, are used. Alternatively or additionally, in certain embodiments, microbes of a fourth Streptomyces griseoincarnatus strain, such as the strain designated OATH00921, are used. Alternatively or additionally, in certain embodiments, microbes of a fifth Streptomyces griseoincarnatus strain, such as the strain designated OATH01245, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces griseoincarnatus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0059] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces griseoviridis, such as microbes of one or more Streptomyces griseoviridis strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces griseoviridis strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces griseoviridis microbes comprise a first microbe of a first Streptomyces griseoviridis strain. In certain embodiments, one or more Streptomyces griseoviridis microbes comprise a second microbe of a second Streptomyces griseoviridis strain, different from the first strain. In certain embodiments, one or more Streptomyces griseoviridis microbes comprise a third microbe of a third Streptomyces griseoviridis strain, different from the first and second strains. In certain embodiments, one or more Streptomyces griseoviridis microbes comprise a fourth microbe of a fourth Streptomyces griseoviridis strain, different from the first, second, and third strains. In certain embodiments,Docket No. OATH-015-PCT2 microbes of a first Streptomyces griseoviridis strain, such as the strain designated OATH01141, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces griseoviridis strain, such as the strain designated OATH01142 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces griseoviridis strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0060] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces olivaceus, such as microbes of one or more Streptomyces olivaceus strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces olivaceus strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces olivaceus microbes comprise a first microbe of a first Streptomyces olivaceus strain. In certain embodiments, one or more Streptomyces olivaceus microbes comprise a second microbe of a second Streptomyces olivaceus strain, different from the first strain. In certain embodiments, one or more Streptomyces olivaceus microbes comprise a third microbe of a third Streptomyces olivaceus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces olivaceus microbes comprise a fourth microbe of a fourth Streptomyces olivaceus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces olivaceus strain, such as the strain designated OATH00351, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces olivaceus strain, such as the strain designated OATH00352 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces olivaceus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0061] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces toxytricini, such as microbes of one or more Streptomyces toxytricini strains, e.g., at least 1, 2, 3, or 4 and / or notDocket No. OATH-015-PCT2 more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces toxytricini strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces toxytricini microbes comprise a first microbe of a first Streptomyces toxytricini strain. In certain embodiments, one or more Streptomyces toxytricini microbes comprise a second microbe of a second Streptomyces toxytricini strain, different from the first strain. In certain embodiments, one or more Streptomyces toxytricini microbes comprise a third microbe of a third Streptomyces toxytricini strain, different from the first and second strains. In certain embodiments, one or more Streptomyces toxytricini microbes comprise a fourth microbe of a fourth Streptomyces toxytricini strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces toxytricini strain, such as the strain designated OATH00011, are used. Alternatively or additionally, in certain embodiments, microbes of a second Streptomyces toxytricini strain, such as the strain designated OATH01091 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces toxytricini strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0062] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces griseoruber, such as microbes of one or more Streptomyces griseoruber strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces griseoruber strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces griseoruber microbes comprise a first microbe of a first Streptomyces griseoruber strain. In certain embodiments, one or more Streptomyces griseoruber microbes comprise a second microbe of a second Streptomyces griseoruber strain, different from the first strain. In certain embodiments, one or more Streptomyces griseoruber microbes comprise a third microbe of a third Streptomyces griseoruber strain, different from the first and second strains. In certain embodiments, one or more Streptomyces griseoruber microbes comprise a fourth microbe of a fourth Streptomyces griseoruber strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces griseoruber strain, such as the strain designated OATH02061, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces griseoruber strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98,Docket No. OATH-015-PCT2 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0063] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces kurssanovii, such as microbes of one or more Streptomyces kurssanovii strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces kurssanovii strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces kurssanovii microbes comprise a first microbe of a first Streptomyces kurssanovii strain. In certain embodiments, one or more Streptomyces kurssanovii microbes comprise a second microbe of a second Streptomyces kurssanovii strain, different from the first strain. In certain embodiments, one or more Streptomyces kurssanovii microbes comprise a third microbe of a third Streptomyces kurssanovii strain, different from the first and second strains. In certain embodiments, one or more Streptomyces kurssanovii microbes comprise a fourth microbe of a fourth Streptomyces kurssanovii strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces kurssanovii strain, such as the strain designated OATH02081, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces kurssanovii strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0064] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces griseofuscus, such as microbes of one or more Streptomyces griseofuscus strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces griseofuscus strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces griseofuscus microbes comprise a first microbe of a first Streptomyces griseofuscus strain. In certain embodiments, one or more Streptomyces griseofuscus microbes comprise a second microbe of a second Streptomyces griseofuscus strain, different from the first strain. In certain embodiments, one or more Streptomyces griseofuscus microbes comprise a third microbe of a third Streptomyces griseofuscus strain, different from the first and second strains. In certain embodiments, one or more Streptomyces griseofuscus microbes comprise a fourth microbe of a fourth StreptomycesDocket No. OATH-015-PCT2 griseofuscus strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces griseofuscus strain, such as the strain designated OATH01254, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces griseofuscus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0065] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces nigra, such as microbes of one or more Streptomyces nigra strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces nigra strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces nigra microbes comprise a first microbe of a first Streptomyces nigra strain. In certain embodiments, one or more Streptomyces nigra microbes comprise a second microbe of a second Streptomyces nigra strain, different from the first strain. In certain embodiments, one or more Streptomyces nigra microbes comprise a third microbe of a third Streptomyces nigra strain, different from the first and second strains. In certain embodiments, one or more Streptomyces nigra microbes comprise a fourth microbe of a fourth Streptomyces nigra strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces nigra strain, such as the strain designated OATH01951, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces nigra strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0066] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces nigrescens, such as microbes of one or more Streptomyces nigrescens strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces nigrescens strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces nigrescens microbes comprise a first microbe of a first Streptomyces nigrescens strain. In certain embodiments, one or more Streptomyces nigrescens microbes comprise a second microbe of a second StreptomycesDocket No. OATH-015-PCT2 nigrescens strain, different from the first strain. In certain embodiments, one or more Streptomyces nigrescens microbes comprise a third microbe of a third Streptomyces nigrescens strain, different from the first and second strains. In certain embodiments, one or more Streptomyces nigrescens microbes comprise a fourth microbe of a fourth Streptomyces nigrescens strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces nigrescens strain, such as the strain designated OATH00913, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Streptomyces nigrescens strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0067] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces sp000721605, such as microbes of one or more Streptomyces sp000721605 strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces sp000721605 strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces sp000721605 microbes comprise a first microbe of a first Streptomyces sp000721605 strain. In certain embodiments, one or more Streptomyces sp000721605 microbes comprise a second microbe of a second Streptomyces sp000721605 strain, different from the first strain. In certain embodiments, one or more Streptomyces sp000721605 microbes comprise a third microbe of a third Streptomyces sp000721605 strain, different from the first and second strains. In certain embodiments, one or more Streptomyces sp000721605 microbes comprise a fourth microbe of a fourth Streptomyces sp000721605 strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces sp000721605 strain, such as the strain designated OATH02071, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A.In certain embodiments, microbe or microbes of Streptomyces sp000721605 strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0068] Additionally or alternatively, in embodiments including one or more Streptomyces microbes, Streptomyces microbes comprise microbes of Streptomyces sp019049245, such asDocket No. OATH-015-PCT2 microbes of one or more Streptomyces sp019049245 strains, e.g., at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, 5, 6, 7, or 8 Streptomyces sp019049245 strains, such as 1-8, preferably 1-7, more preferably 1-5 In certain embodiments, one or more Streptomyces sp019049245 microbes comprise a first microbe of a first Streptomyces sp019049245 strain. In certain embodiments, one or more Streptomyces sp019049245 microbes comprise a second microbe of a second Streptomyces sp019049245 strain, different from the first strain. In certain embodiments, one or more Streptomyces sp019049245 microbes comprise a third microbe of a third Streptomyces sp019049245 strain, different from the first and second strains. In certain embodiments, one or more Streptomyces sp019049245 microbes comprise a fourth microbe of a fourth Streptomyces sp019049245 strain, different from the first, second, and third strains. In certain embodiments, microbes of a first Streptomyces sp019049245 strain, such as the strain designated OATH02091, are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A.In certain embodiments, microbe or microbes of Streptomyces sp019049245 strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0069] In embodiments including one or more non-Streptomyces microbes, any suitable non- Streptomyces microbes may be used. In certain embodiments, microbes of one or more non- Streptomyces strain comprise microbes of one or more of Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces, Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, and / or Zhihengliuella
[0070] In embodiments including microbes of one or more Pseudomonas strains, any suitable species and / or strain(s) of Pseudomonas may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, 4, 5, or 6 and / or not more than 2, 3, 4, 5, 6, or 7, preferably 1-7, more preferably 1-5, even more preferably 1-3, such as 1, or 2, Pseudomonas strains are used. In certain embodiments, one or more Pseudomonas microbes comprise a first microbe of a first Pseudomonas strain. In certain embodiments, one or more Pseudomonas microbes comprise a second microbe of a second Pseudomonas strain, different from the first strain. In certain embodiments, microbes of a first Pseudomonas E paracarnis strain, such as the strain designated OATH00102, are used. Alternatively or additionally, in certain embodiments, microbes of aDocket No. OATH-015-PCT2 second Pseudomonas E paracarnis strain, such as the strain designated OATH01112 are used. Alternatively or additionally, in certain embodiments, microbes of a first Pseudomonas delhiensis strain, such as the strain designated OATH01601, are used. Alternatively or additionally, in certain embodiments, microbes of a second Pseudomonas delhiensis strain, such as the strain designated OATH01651 are used. Alternatively or additionally, in certain embodiments, microbes of a first Pseudomonas_K oryzae strain, such as the strain designated OATH01461, are used. Alternatively or additionally, in certain embodiments, microbes of a second Pseudomonas_K oryzae strain, such as the strain designated OATH01431 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Pseudomonas strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0071] In embodiments including microbes of one or more Microvirga strains, any suitable strain(s) of Microvirga may be used. In certain embodiments, the Microvirga strain comprises Microvirga sp022346925, such as the strain designated OATH01971 .16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe(s) of the Microvirga strain have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0072] In embodiments including microbes of one or more Paenibacillus strains, any suitable strain(s) of Paenibacillus may be used. In certain embodiments, cells (microbes) of at least 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6 Paenibacillus strains are used. In certain embodiments, one or more Paenibacillus microbes comprise a first microbe of a first Paenibacillus strain. In certain embodiments, one or more Paenibacillus microbes comprise a second microbe of a second Paenibacillus strain, different from the first strain. In certain embodiments, one or more Paenibacillus microbes comprise a third microbe of a third Paenibacillus strain, different from the first and second strains. In certain embodiments, microbes of a first Paenibacillus cellulositrophicus strain, such as the strain designated as OATH00811, are used. Alternatively or additionally, in certain embodiments, microbes of aDocket No. OATH-015-PCT2 second Paenibacillus cellulositrophicus strain, designated as OATH01263 are used. Alternatively or additionally, in certain embodiments, microbes of a third Paenibacillus cellulositrophicus strain, designated as OATH01371 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Paenibacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0073] In embodiments including microbes of one or more Rhodococcus strains, any suitable strain(s) of Rhodococcus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6 Rhodococcus strains are used. In certain embodiments, one or more Rhodococcus microbes comprise a first microbe of a first Rhodococcus strain. In certain embodiments, one or more Rhodococcus microbes comprise a second microbe of a second Rhodococcus strain, different from the first strain. In certain embodiments, microbes of Rhodococcus strains comprise microbes of one or more of Rhodococcus erythropolis strains, such as Rhodococcus erythropolis designation OATH00923 are used. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Rhodococcus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100% identity, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0074] In embodiments including microbes of one or more Lysinibacillus strains, any suitable strain(s) of Lysinibacillus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 Lysinibacillus strains are used. In certain embodiments, one or more Lysinibacillus microbes comprise a first microbe of a first Lysinibacillus strain. In certain embodiments, one or more Lysinibacillus microbes comprise a second microbe of a second Lysinibacillus strain, different from the first strain. In certain embodiments, microbes of Lysinibacillus strains comprise microbes of Lysinibacillus capsici, such as Lysinibacillus capsici designation OATH01003, are used. Alternatively or additionally, in certain embodiments, microbes of Lysinibacillus strains comprise microbes of Lysinibacillus sphaericus, such as Lysinibacillus sphaericus designation OATH00892, are used. 16S rRNADocket No. OATH-015-PCT2 sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Lysinibacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0075] In embodiments including microbes of one or more Bacillus strains, any suitable strain(s) of Bacillus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 15, 17, 20, or 22 and / or not more than 1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 15, 17, 20, 22, 25, or 30 Bacillus strains are used. In certain embodiments, one or more Bacillus microbes comprise a first microbe of a first Bacillus strain. In certain embodiments, one or more Bacillus microbes comprise a second microbe of a second Bacillus strain, different from the first strain. In certain embodiments, one or more Bacillus microbes comprise a third microbe of a third Bacillus strain, different from the first and second strains. In certain embodiments, one or more Bacillus microbes comprise a fourth microbe of a fourth Bacillus strain, different from the first, second, and third strains. In certain embodiments, one or more Bacillus microbes comprise a fifth microbe of a fifth Bacillus strain, different from the first, second, third, and fourth strains. In certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus haynesii such as Bacillus haynesii designation OATH01252 are used. Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus inaquosorum such as Bacillus inaquosorum designation OATH01621, and / or Bacillus inaquosorum designation OATH01961, are used. Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus licheniformis such as Bacillus licheniformis designation OATH01291 Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus paralicheniformis such as Bacillus paralicheniformis designation OATH02131 and / or Bacillus paralicheniformis designation OATH02151 Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus safensis such as Bacillus safensis designation OATH01491 , and / or Bacillus safensis designation OATH01611 Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus spizizenii such as Bacillus spizizenii designation OATH01881 , Bacillus spizizenii designation OATH01991 ,and / or Bacillus spizizenii designation OATH02001 Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus subtilis such as Bacillus subtilis designation OATH01511 , Bacillus subtilis designation OATH01561 , BacillusDocket No. OATH-015-PCT2 subtilis designation OATH01631 Bacillus subtilis designation OATH01681 and / or Bacillus subtilis designation OATH01751 Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus velizensis such as Bacillus velizensis designation OATH01382 , and / or Bacillus velizensis designation OATH01383 . Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus_A thuringiensis_K such as Bacillus_A thuringiensis_K designation OATH01441. Alternatively or additionally, in certain embodiments, microbes of Bacillus strains comprise microbes of Bacillus_I endophytica_A such as Bacillus_I endophytica_A designation OATH01471 , Bacillus_I endophytica_A designation OATH01481 , Bacillus_I endophytica_A designation OATH01521 , and / or Bacillus_I endophytica_A designation OATH01641.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Bacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity t to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0076] In embodiments including microbes of one or more Aneurinibacillus strains, any suitable strain(s) of Aneurinibacillus may be used. In certain embodiments, cells (microbes) of at least 1, 2, or 3 and / or not more than 2, 3, or 4 Aneurinibacillus strains are used. In certain embodiments, one or more Aneurinibacillus microbes comprise a first microbe of a first Aneurinibacillus strain. In certain embodiments, one or more Aneurinibacillus microbes comprise a second microbe of a second Aneurinibacillus strain, different from the first strain. In certain embodiments, microbes of Aneurinibacillus strains comprise microbes of Aneurinibacillus aneurinilyticus, such as Aneurinibacillus aneurinilyticus designation OATH01281.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Aneurinibacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0077] In embodiments including microbes of one or more Brevibacillus strains, any suitable strain(s) of Brevibacillus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 Brevibacillus strains are used. In certain embodiments,Docket No. OATH-015-PCT2 one or more Brevibacillus microbes comprise a first microbe of a first Brevibacillus strain. In certain embodiments, one or more Brevibacillus microbes comprise a second microbe of a second Brevibacillus strain, different from the first strain. In certain embodiments, microbes of Brevibacillus strains comprise microbes of Brevibacillus panacihumi, such as Brevibacillus panacihumi designation OATH01363.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Brevibacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0078] In embodiments including microbes of one or more Caldimonas strains, any suitable strain(s) of Caldimonas may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 Caldimonas strains are used. In certain embodiments, one or more Caldimonas microbes comprise a first microbe of a first Caldimonas strain. In certain embodiments, one or more Caldimonas microbes comprise a second microbe of a second Caldimonas strain, different from the first strain. In certain embodiments, microbes of Caldimonas strains comprise microbes of Caldimonas such as Caldimonas mangrovi designation OATH01801.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Caldimonas strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0079] In embodiments including microbes of one or more Actinoplanes strains, any suitable strain(s) of Actinoplanes may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Actinoplanes microbes comprise a first microbe of a first Actinoplanes strain. In certain embodiments, one or more Actinoplanes microbes comprise a second microbe of a second Actinoplanes strain, different from the first strain. In certain embodiments, microbes of Actinoplanes strains comprise microbes of Actinoplanes aureus, such as Actinoplanes aureus designation OATH01911. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of ActinoplanesDocket No. OATH-015-PCT2 strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0080] In embodiments including microbes of one or more Agromyces strains, any suitable strain(s) of Agromyces may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Agromyces microbes comprise a first microbe of a first Agromyces strain. In certain embodiments, one or more Agromyces microbes comprise a second microbe of a second Agromyces strain, different from the first strain. In certain embodiments, microbes of Agromyces strains comprise microbes of Agromyces sp031178025, such as Agromyces sp031178025 designation OATH01981. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Agromyces strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0081] In embodiments including microbes of one or more Allosphingosinicella strains, any suitable strain(s) of Allosphingosinicella may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Allosphingosinicella microbes comprise a first microbe of a first Allosphingosinicella strain. In certain embodiments, one or more Allosphingosinicella microbes comprise a second microbe of a second Allosphingosinicella strain, different from the first strain. In certain embodiments, microbes of Allosphingosinicella strains comprise microbes of Allosphingosinicella sp011320075, such as Allosphingosinicella sp011320075 designation OATH02101 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Allosphingosinicella strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.Docket No. OATH-015-PCT2
[0082] In embodiments including microbes of one or more Arthrobacter strains, any suitable strain(s) of Arthrobacter may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, 4, 5, 6, or 7 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, or 10 strains are used. In certain embodiments, one or more Arthrobacter microbes comprise a first microbe of a first Arthrobacter strain. In certain embodiments, one or more Arthrobacter microbes comprise a second microbe of a second Arthrobacter strain, different from the first strain. In certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter nitrophenolicus , such as Arthrobacter nitrophenolicus designation OATH01591. Alternatively or additionally, in certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter pascens such as Arthrobacter pascens designation OATH01671.. Alternatively or additionally, in certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter phenanthrenivorans such as Arthrobacter phenanthrenivorans designation OATH01811. Alternatively or additionally, in certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter siccitolerans such as Arthrobacter siccitolerans designation OATH01571. Alternatively or additionally, in certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter sp006715285 such as Arthrobacter sp006715285 designation OATH01891. Alternatively or additionally, in certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter sp019977195 such as Arthrobacter sp019977195 designation OATH01711. Alternatively or additionally, in certain embodiments, microbes of Arthrobacter strains comprise microbes of Arthrobacter sp021129155 such as Arthrobacter sp021129155 designation OATH01731 and / or Arthrobacter sp021129155 designation OATH01721.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Arthrobacter strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0083] In embodiments including microbes of one or more Cupriavidus strains, any suitable strain(s) of Cupriavidus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Cupriavidus microbes comprise a first microbe of a first Cupriavidus strain. In certain embodiments, one or more Cupriavidus microbes comprise a second microbe of a second Cupriavidus strain, different from the first strain. In certain embodiments, microbes of Cupriavidus strains comprise microbes of Cupriavidus laharis, such as Cupriavidus laharisDocket No. OATH-015-PCT2 designation OATH01501. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Cupriavidus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0084] In embodiments including microbes of one or more Erythrobacter strains, any suitable strain(s) of Erythrobacter may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Erythrobacter microbes comprise a first microbe of a first Erythrobacter strain. In certain embodiments, one or more Erythrobacter microbes comprise a second microbe of a second Erythrobacter strain, different from the first strain. In certain embodiments, microbes of Erythrobacter strains comprise microbes of Erythrobacter sp006542605 , such as Erythrobacter sp006542605 designation OATH2021. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Erythrobacter strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0085] In embodiments including microbes of one or more Kocuria strains, any suitable strain(s) of Kocuria may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Kocuria microbes comprise a first microbe of a first Kocuria strain. In certain embodiments, one or more Kocuria microbes comprise a second microbe of a second Kocuria strain, different from the first strain. In certain embodiments, microbes of Kocuria strains comprise microbes of Kocuria polaris_A, such as Kocuria polaris_A designation OATH01831 , Kocuria polaris_A designation OATH01871, and / or Kocuria polaris_A designation OATH01901.. Alternatively or additionally, in certain embodiments, microbes of Kocuria strains comprise microbes of Kocuria turfanensis such as Kocuria turfanensis designation OATH01781. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Kocuria strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%,Docket No. OATH-015-PCT2 such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0086] In embodiments including microbes of one or more Microbacterium strains, any suitable strain(s) of Microbacterium may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Microbacterium microbes comprise a first microbe of a first Microbacterium strain. In certain embodiments, one or more Microbacterium microbes comprise a second microbe of a second Microbacterium strain, different from the first strain. In certain embodiments, microbes of Microbacterium strains comprise microbes of Microbacterium oxydans, such as Microbacterium oxydans designation OATH00143. Alternatively or additionally, in certain embodiments, microbes of Microbacterium strains comprise microbes of Microbacterium paraoxydans such as Microbacterium paraoxydans designation OATH00224 and / or Microbacterium paraoxydans designation OATH00483.. Alternatively or additionally, in certain embodiments, microbes of Microbacterium strains comprise microbes of Microbacterium resistens such as Microbacterium resistens designation OATH00342 Alternatively or additionally, in certain embodiments, microbes of Microbacterium strains comprise microbes of Microbacterium sp002979655 such as Microbacterium sp002979655 designation OATH00493 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Microbacterium strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0087] In embodiments including microbes of one or more Microbispora strains, any suitable strain(s) of Microbisporamay be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Microbisporamicrobes comprise a first microbe of a first Microbisporastrain. In certain embodiments, one or more Microbisporamicrobes comprise a second microbe of a second Microbisporastrain, different from the first strain. In certain embodiments, microbes of Microbisporastrains comprise microbes of Microbispora sp014161445, such as Microbispora sp014161445designation OATH02011. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes ofDocket No. OATH-015-PCT2 Microbispora strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0088] In embodiments including microbes of one or more Mycetocola strains, any suitable strain(s) of Mycetocola may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Mycetocola microbes comprise a first microbe of a first Mycetocola strain. In certain embodiments, one or more Mycetocola microbes comprise a second microbe of a second Mycetocola strain, different from the first strain. In certain embodiments, microbes of Mycetocola strains comprise microbes of Mycetocola_A miduiensis such as Mycetocola_A miduiensis designation OATH02111. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Mycetocola strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0089] In embodiments including microbes of one or more Neorhizobium strains, any suitable strain(s) of Neorhizobium may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Neorhizobium microbes comprise a first microbe of a first Neorhizobium strain. In certain embodiments, one or more Neorhizobium microbes comprise a second microbe of a second Neorhizobium strain, different from the first strain. In certain embodiments, microbes of Neorhizobium strains comprise microbes of Neorhizobium flavum, such as Neorhizobium flavum designation OATH01861 , Neorhizobium flavum designation OATH01931., and / or Neorhizobium flavum designation OATH01941. Alternatively or additionally, in certain embodiments, microbes of Neorhizobium strains comprise microbes of Neorhizobium petrolearium such as Neorhizobium petrolearium designation OATH01921. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Neorhizobium strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even moreDocket No. OATH-015-PCT2 preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0090] In embodiments including microbes of one or more Nocardia strains, any suitable strain(s) of Nocardia may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Nocardia microbes comprise a first microbe of a first Nocardia strain. In certain embodiments, one or more Nocardia microbes comprise a second microbe of a second Nocardia strain, different from the first strain. In certain embodiments, microbes of Nocardia strains comprise microbes of Nocardia farcinica, such as Nocardia farcinica designation OATH01771. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Nocardia strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0091] In embodiments including microbes of one or more Nocardioides strains, any suitable strain(s) of Nocardioides may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Nocardioides microbes comprise a first microbe of a first Nocardioides strain. In certain embodiments, one or more Nocardioides microbes comprise a second microbe of a second Nocardioides strain, different from the first strain. In certain embodiments, microbes of Nocardioides strains comprise microbes of Nocardioides strain designation OATH02121.16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Nocardioides strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0092] In embodiments including microbes of one or more Nonomuraea strains, any suitable strain(s) of Nonomuraea may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Nonomuraea microbes comprise a first microbe of a first Nonomuraea strain. In certainDocket No. OATH-015-PCT2 embodiments, one or more Nonomuraea microbes comprise a second microbe of a second Nonomuraea strain, different from the first strain. In certain embodiments, microbes of Nonomuraea strains comprise microbes of Nonomuraea rhodomycinica, such as Nonomuraea rhodomycinica designation OATH01761. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Nonomuraea strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0093] In embodiments including microbes of one or more Peribacillus strains, any suitable strain(s) of Peribacillus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Peribacillus microbes comprise a first microbe of a first Peribacillus strain. In certain embodiments, one or more Peribacillus microbes comprise a second microbe of a second Peribacillus strain, different from the first strain. In certain embodiments, microbes of Peribacillus strains comprise microbes of Peribacillus frigoritolerans_A, such as Peribacillus frigoritolerans_A designation OATH01661 , Peribacillus frigoritolerans_A designation OATH01691., and / or Peribacillus frigoritolerans_A designation OATH01741 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Peribacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0094] In embodiments including microbes of one or more Priestia strains, any suitable strain(s) of Priestia may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Priestia microbes comprise a first microbe of a first Priestia strain. In certain embodiments, one or more Priestia microbes comprise a second microbe of a second Priestia strain, different from the first strain. In certain embodiments, microbes of Priestia strains comprise microbes of Priestia aryabhattai, such as Priestia aryabhattai designation OATH01451 , Priestia aryabhattai designation OATH01531 , Priestia aryabhattai designation OATH01541, Priestia aryabhattaiDocket No. OATH-015-PCT2 designation OATH01841, and / or Priestia aryabhattai designation OATH01551 Alternatively or additionally, in certain embodiments, microbes of Priestia strains comprise microbes of Priestia megaterium such as Priestia megaterium designation OATH01581. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Priestia strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0095] In embodiments including microbes of one or more Pristimantibacillus strains, any suitable strain(s) of Pristimantibacillus may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Pristimantibacillus microbes comprise a first microbe of a first Pristimantibacillus strain. In certain embodiments, one or more Pristimantibacillus microbes comprise a second microbe of a second Pristimantibacillus strain, different from the first strain. In certain embodiments, microbes of Pristimantibacillus strains comprise microbes of Pristimantibacillus strain designation OATH01272. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Pristimantibacillus strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0096] In embodiments including microbes of one or more Stenotrophomonas strains, any suitable strain(s) of Stenotrophomonas may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Stenotrophomonas microbes comprise a first microbe of a first Stenotrophomonas strain. In certain embodiments, one or more Stenotrophomonas microbes comprise a second microbe of a second Stenotrophomonas strain, different from the first strain. In certain embodiments, microbes of Stenotrophomonas strains comprise microbes of Stenotrophomonas geniculata, such as Stenotrophomonas geniculata designation OATH01181. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Stenotrophomonas strains have a 16S rRNA geneDocket No. OATH-015-PCT2 nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A.
[0097] In embodiments including microbes of one or more Zhihengliuella strains, any suitable strain(s) of Zhihengliuella may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Zhihengliuella microbes comprise a first microbe of a first Zhihengliuella strain. In certain embodiments, one or more Zhihengliuella microbes comprise a second microbe of a second Zhihengliuella strain, different from the first strain. In certain embodiments, microbes of Zhihengliuella strains comprise microbes of Zhihengliuella sp031178195, such as Zhihengliuella sp031178195 designation OATH01701. 16S rRNA sequences and ATCC Deposit numbers including each strain are as shown in Table 2A. In certain embodiments, microbe or microbes of Zhihengliuella strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A..
[0098] In embodiments including microbes of one or more Stutzerimonas strains, any suitable strain(s) of Stutzerimonas may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3, or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Stutzerimonas microbes comprise a first microbe of a first Stutzerimonas strain. In certain embodiments, one or more Stutzerimonas microbes comprise a second microbe of a second Stutzerimonas strain, different from the first strain. In certain embodiments, microbes of Stutzerimonas strains comprise microbes of Stutzerimonas stutzeri_AI, such as Stutzerimonas stutzeri_AI designation OATH01791. In certain embodiments, microbe or microbes of Stutzerimonas strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0099] In embodiments including microbes of one or more Terrimonas strains, any suitable strain(s) of Terrimonas may be used. In certain embodiments, cells (microbes) of at least 1, 2, 3,Docket No. OATH-015-PCT2 or 4 and / or not more than 2, 3, 4, or 5 strains are used. In certain embodiments, one or more Terrimonas microbes comprise a first microbe of a first Terrimonas strain. In certain embodiments, one or more Terrimonas microbes comprise a second microbe of a second Terrimonas strain, different from the first strain. In certain embodiments, microbes of ____________strains comprise microbes of Terrimonas designation OATH01821. In certain embodiments, microbe or microbes of Terrimonas strains have a 16S rRNA gene nucleotide sequence or sequences with at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.55 identity, or 100%, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to one or more sequences for each strain as designated in Table 2A to one or more sequences for each strain as designated in Table 2A.
[0100] In certain embodiments, provided is a composition comprising microbes from the genera Microbacterium, Rhodococcus, and Streptomyces. Streptomyces can include one or more strains of the species Streptomyces griseoincarnatus, Streptomyces misionensis, Streptomyces murinus, Streptomyces echinatu, Streptomyces bacillari, Streptomyces sennicomposti, Streptomyces chartreusis, Streptomyces globosus, Streptomyces achromogenes, and / or Streptomyces eurythermus. Microbacterium can include one or more strains of the species Microbacterium paraoxydans . Rhodococcus can include one or more strains of the species Rhodococcus erythropolis. The composition can comprise one or more of the bacteria of Table 33, Example 36. The composition may further comprise one or more microbes of the genera Microbacterium, such as Microbacterium sp002979655, for example OATH00493 comprising a 16S rRNA sequence of SEQ ID No.374. The composition may further comprise Streptomyces griseoincarnatus OATH00921 comprising a 16S rRNA sequence of SEQ ID No.398. The composition may further comprise one or more microbes of the genera Bacillus, Brevibacillus, Lysinibacillus, and / or Paenibacillus. Exemplary species include but are not limited to Bacillus velezensis, Brevibacillus panacihumi, Lysinibacillus sphaericus, and Paenibacillus cellulositrophicus. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.
[0101] In certain embodiments, provided is a composition comprising microbes from the genera Lysinibacillus, Microbacterium, Rhodococcus, and Streptomyces. Streptomyces can include one or more strains of the species Streptomyces griseoincarnatus, Streptomyces griseoaurantiacus, Streptomyces rochei, Streptomyces bikiniensis, Streptomyces misionensis, Streptomyces murinus, Streptomyces bacillari, Streptomyces sennicomposti, and / or Streptomyces achromogenes. Lysinibacillus can include one or more strains of the species Lysinibacillus sphaericus. Microbacterium can include one or more strains of the species Microbacterium paraoxydans. Rhodococcus can include one or more strains of the species RhodococcusDocket No. OATH-015-PCT2 erythropolis. The composition can comprise one or more of the bacteria of Table 34, Example 37. The composition may further comprise one or more microbes of the genera Microbacterium, such as Microbacterium sp002979655, for example OATH00493 comprising a 16S rRNA sequence of SEQ ID No. 374. The composition may further comprise Streptomyces griseoincarnatus OATH00921 comprising a 16S rRNA sequence of SEQ ID No.398 and / or Strepomyces grisoincarnatus OATH00412 comprising a 16S rRNA sequence comprising SEQ ID NOs.367, 368, and 369. The composition may further comprise one or more microbes of the genera Aneurinibacillus, Bacillus, Brevibacillus, and / or Paenibacillus. Exemplary species include but are not limited to Aneurinibacillus aneurinilyticus, Bacillus haynesii, Bacillus licheniformis, Bacillus velezensis, Brevibacillus panacihumi, Lysinibacillus capsici, and Paenibacillus cellulositrophicus. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.
[0102] In certain embodiments, provided is a composition comprising microbes from the genera Lysinibacillus, Microbacterium, Rhodococcus, and Streptomyces. Streptomyces can include one or more strains of the species Streptomyces misionensis, Streptomyces bacillari, Streptomyces achromogenes, and / or Streptomyces globosus,. Lysinibacillus can include one or more strains of the species Lysinibacillus sphaericus. Microbacterium can include one or more strains of the species Microbacterium paraoxydans. Rhodococcus can include one or more strains of the species Rhodococcus erythropolis. The composition can comprise one or more of the bacteria of Table 37, Example 40. The composition may further comprise one or more microbes of the genera Microbacterium, such as Microbacterium sp002979655, for example OATH00493 comprising a 16S rRNA sequence of SEQ ID No.374. The composition may further comprise one or more microbes of the genera Aneuinibacillus, Bacillus, Brevibacillus, and / or Paenibacillus. Exemplary species include but are not limited to Aneurinibacillus aneurinilyticus, Bacillus haynesii, Bacillus licheniformis, Bacillus velezensis, Brevibacillus panacihumi, Lysinibacillus capsici, Paenibacillus cellulositrophicus. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.
[0103] In certain embodiments, provided is a composition comprising microbes from the genera Bacillus, Lysinibacillus, Rhodococcus, and Streptomyces. Streptomyces can include one or more strains of the species Streptomyces misionensis, Streptomyces bacillari, Streptomyces achromogenes, Streptomyces globosus, Streptomyces antibioticus, Streptomyces bikiniensis, Streptomyces chartreusis Streptomyces echinatu, Streptomyces griseoaurantiacus, Streptomyces murinus, Streptomyces musisoli, Streptomyces olivaceus, Streptomyces sennicomposti, and / or Streptomyces toxytricini. Bacillus can include one or more strains of the species Bacillus velizensis. Lysinibacillus can include one or more strains of the species Lysinibacillus sphaericus.Docket No. OATH-015-PCT2 Rhodococcus can include one or more strains of the species Rhodococcus erythropolis. The composition can comprise one or more of the bacteria of Table 38, Example 41. The composition may further comprise one or more microbes of the genera Microbacterium, such as Microbacterium sp002979655, for example OATH00493 comprising a 16S rRNA sequence of SEQ ID No.374. The composition may further comprise Streptomyces griseoincarnatus OATH00412 comprising a 16S rRNA sequence comprising SEQ ID NOs.367, 368, and 369. The composition may further comprise one or more microbes of the genera Aneurinibacillus, Brevibacillus, Microbacterium, and / or Paenibacillus. Exemplary species include but are not limited to Aneurinibacillus aneurinilyticus, Bacillus haynesii, Bacillus licheniformis, Brevibacillus panacihumi, Lysinibacillus capsici, Microbacterium azadirachtae, Microbacterium oxydans, Paenibacillus cellulositrophicus, Paenibacillus thailandensis, Streptomyces eurythermus, and Streptomyces griseostramineus. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.
[0104] In certain embodiments, provided is a composition comprising microbes from the genera Lysinibacillus, Microbacterium, Rhodococcus, and Streptomyces. Streptomyces can include one or more strains of the species Streptomyces achromogenes, Streptomyces bacillari, Streptomyces globosus, Streptomyces misionensis, and / or Streptomyces rochei. Lysinibacillus can include one or more strains of the species Lysinibacillus sphaericus. Rhodococcus can include one or more strains of the species Rhodococcus erythropolis. Microbacterium can include one or more strains of the species Microbacterium paraoxydans. The composition can comprise one or more of the bacteria of Table 40, Example 43. The composition may further comprise one or more microbes of the genera Bacillus, Paenibacillus, Pisolithus, Rhizopogon, and / or Scleroderma. Exemplary species include but are not limited to Bacillus haynesii, Bacillus licheniformis, Bacillus velezensis, Paenibacillus cellulositrophicus, Pisolithus tinctorius, Rhizopogon luteolus, and Scleroderma citrinum. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein, for example, ectomycorrhizal fungi such as one or more of ectomycorrhizal fungi of one more genera of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, and / or Sebacina, as described further herein.
[0105] In certain embodiments, provided is a composition comprising microbes from the genera Microbacterium, Rhodococcus, and Streptomyces. Microbacterium can include one or more strains of the species Microbacterium paraoxydans. Rhodococcus can include one or more strains of the species Rhodococcus erythropolis. Streptomyces can include one or more strains of the species Streptomyces achromogenes, Streptomyces bacillari, Streptomyces bikiniensis ,Streptomyces globosus, Streptomyces griseoincarnatus, Streptomyces misionensis, StreptomycesDocket No. OATH-015-PCT2 murinus, and / or Streptomyces rochei. The composition can comprise one or more of the bacteria of Table 41, Example 44. The composition may further comprise one or more microbes of the genera Microbacterium, such as Microbacterium sp002979655, for example OATH00493 comprising a 16S rRNA sequence of SEQ ID No.374. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein, for example, ectomycorrhizal fungi such as one or more of ectomycorrhizal fungi of one more genera of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, and / or Sebacina, as described further herein.
[0106] In certain embodiments, provided is a composition comprising microbes from the genera Bacillus, Lysinibacillus, Microbacterium, Neorhizobium, Peribacillus, Priestia, Pseudomonas, Rhodococcus, and Streptomyces. Streptomyces can include one or more strains of the species Streptomyces nigrescens, Streptomiyces nigra, Streptomyces bobili, Streptomyces albogriseolus, Streptomyces globosus, Streptomyces griseoincarnatus, Streptomyces griseoaurantiacus, Streptomyces rochei, Streptomyces bikiniensis, Streptomyces misionensis, Streptomyces murinus, Streptomyces bacillari, Streptomyces sennicomposti, and / or Streptomyces achromogenes. Bacillus can include one or more strains of the species Bacillus spizizenii and / or Bacillus paralicheniformis Lysinibacillus can include one or more strains of the species Lysinibacillus sphaericus Microbacterium can include one or more strains of the species Microbacterium paraoxydans Neorhizobium can include one or more strains of the species Neorhizobium flavum. Peribacillus can include one or more strains of the species Peribacillus frigoritolerans_A. Priestia can include one or more strains of the species Priestia aryabhattai Pseudomonas can include one or more strains of the species Pseudomonas paracanis Rhodococcus can include one or more strains of the species Rhodococcus erythropolis. The composition can comprise one or more of the bacteria of Table 35, Example 38. The composition may further comprise one or more microbes of the genera Aneurinibacillus, Brevibacillus, Glomus, Paenibacillus, Pisolithus, and / or Rhizopogon. Exemplary species include but are not limited to Aneurinibacillus aneurinilyticus, Bacillus haynesii, Bacillus licheniformis, Bacillus velezensis, Brevibacillus panacihumi, Glomus aggregatum, Glomus etunicatum, Glomus intraradices, Glomus mosseae, Lysinibacillus capsici, Neorhizobium flavum, Paenibacillus cellulositrophicus, Paenibacillus thailandensis, Peribacillus frigoritolerans, Pisolithus tinctorius, Priestia aryabhattai, Priestia megaterium, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon luteolus, Rhizopogon villosullus, and Streptomyces nigrescens. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.Docket No. OATH-015-PCT2
[0107] In certain embodiments, provided is a composition comprising microbes from the genera Aneurinibacillus, Microbacterium, Pseudomonas, Rhodococcus, and Streptomyces. Aneurinibacillus can comprise one or more strains of the species Aneurinibacillus aneurinilyticus. Microbacterium can comprise one or more strains of the species Microbacterium paraoxydans. Pseudomonas can comprise one or more strains of the species Pseudomonas paracanis. Rhodococcus can comprise one or more strains of the species Rhodococcus erythropolis. Streptomyces can comprise one or more strains of the species Streptomyces achromogenes, Streptomyces antibioticus, Streptomyces bacillari, Streptomyces bikiniensis ,Streptomyces chartreusis, Streptomyces echinatu, Streptomyces echinatus, Streptomyces eurythermus, Streptomyces globosus, Streptomyces griseoaurantiacus, Streptomyces misionensis, Streptomyces murinus, Streptomyces musisoli, Streptomyces olivaceus, Streptomyces rochei, Streptomyces sennicomposti, and / or Streptomyces toxytricini. The composition can comprise one or more of the bacteria of Table 42, Example 45. The composition may further comprise Streptomyces griseoincarnatus OATH00412 comprising a 16S rRNA sequence comprising SEQ ID NOs.367, 368, and 369. The composition may further comprise one or more microbes of the genera Bacillus, Lysinibacillus, and / or Paenibacillus. Exemplary species include but are not limited to Bacillus licheniformis, Bacillus velezensis, Lysinibacillus capsici, Microbacterium oxydans, Paenibacillus cellulositrophicus, Paenibacillus thailandensis, Streptomyces eurythermus, and Streptomyces griseostramineus. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein. In certain embodiments, provided is a composition comprising microbes from the genera Aneurinibacillus, Bacillus, Microbacterium, Pseudomonas, Rhodococcus, and Streptomyces. Aneurinibacillus can comprise one or more strains of the species Aneurinibacillus aneurinilyticus. Bacillus can comprise one or more strains of the species Bacillus velizensis. Microbacterium can comprise one or more strains of the species Microbacterium paraoxydans. Pseudomonas can comprise one or more strains of the species Pseudomonas paracanis. Rhodococcus can comprise one or more strains of the species Rhodococcus erythropolis. Streptomyces can comprise one or more strains of the species Streptomyces achromogenes, Streptomyces antibioticus, Streptomyces bacillari, Streptomyces bikiniensis, Streptomyces chartreusis, Streptomyces echinatu, Streptomyces echinatus, Streptomyces eurythermus, Streptomyces globosus, Streptomyces griseoaurantiacus, Streptomyces misionensis, Streptomyces murinus, Streptomyces musisoli, Streptomyces olivaceus, Streptomyces rochei, Streptomyces sennicomposti, and / or Streptomyces toxytriciniThe composition can comprise one or more of the bacteria of Table 43, Example 46.The composition may further comprise one or more microbes of the genera Microbacterium, such as Microbacterium sp002979655, forDocket No. OATH-015-PCT2 example OATH00493 comprising a 16S rRNA sequence of SEQ ID No.374. The composition may further comprise Streptomyces griseoincarnatus OATH00412 comprising a 16S rRNA sequence comprising SEQ ID NOs. 367, 368, and 369. The composition may further comprise one or more microbes of the genera Brevibacillus, Lysinibacillus, Microbacterium, and / or Paenibacillus. Exemplary species include but are not limited to Aneurinibacillus aneurinilyticus, Bacillus haynesii, Bacillus licheniformis, Bacillus velezensis, Brevibacillus panacihumi, Lysinibacillus capsici, Lysinibacillus sphaericus, Microbacterium azadirachtae, Microbacterium oxydans, Paenibacillus cellulositrophicus, Paenibacillus thailandensis, Streptomyces eurythermus, Streptomyces griseoincarnatus, and Streptomyces griseostramineus. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.
[0108] In certain embodiments, provided herein is a composition comprising bacteria of the genus Streptomyces and bacteria of the class Bacilli, e.g., bacteria of one or more of Aneurinibacillus, e.g., one or more strains of the species Aneurinibacillus aneurinilyticus; Bacillus, e.g., one or more strains of the species Bacillus_I endophytica_A, Bacillus haynesii, Bacillus inaqosorum, Bacillus licheniformis, Bacillus paralicheniformis, Bacillus safensis, Bacillus spizizenii, Bacillus subtilis, Bacillus_A thuringiensis_K, and / or Bacillus velizensis; Brevibacillus, such as one or more strains of the species Brevibacillus panacihumi; Lysinibacillus, such as one or more strains of the species Lysinibacillus sphaericus and / or Lysinibacillus capsica; Paenibacillus, such as one or more strains of the species Paenibacillus cellulositrophicus; Peribacillus, such as one or more strains of the species Peribacillus frigoritolerans_A; and / or Pristimantibacillus. In certain embodiments, the composition comprises one or more of the Streptomyces species or strains of the species as shown in Table 2A and one or more of the Bacilli genera, species or strains of the species as shown in Table 2A. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.
[0109] In certain embodiments, provided is a composition comprising bacteria from one or more of genera Actinoplanes, Agromyces, Allosphingosinicella, Arthrobacter, Bacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbispora, Microvirga, Mycetocola, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pseudomonas, Streptomyces, Stutzerimonas, Terrimonas, and / or Zhihengliuella, such as one or more of the strains of Table 44, Example 47. In certain embodiments, one or more or all of these bacteria may be free-living nitrogen-fixing bacteria. In certain embodiments the composition comprises one or more fungi, such as one or more of the fungi disclosed herein.Docket No. OATH-015-PCT2
[0110] In certain embodiments, provided herein is a composition comprising one or more bacteria from one or more of ATCC deposit numbers PTA-127834-PTA-127850, as shown in Table 2A, e.g., one or more bacteria of ATCC deposit number PTA-127834, and / or one or more bacteria of ATCC deposit number PTA-127835, and / or one or more bacteria of ATCC deposit number PTA-127836, and / or one or more bacteria of ATCC deposit number PTA-127837, and / or one or more bacteria of ATCC deposit number PTA-127838, and / or one or more bacteria of ATCC deposit number PTA-127839, and / or one or more bacteria of ATCC deposit number PTA- 127840, and / or one or more bacteria of ATCC deposit number PTA-127841, and / or one or more bacteria of ATCC deposit number PTA-127842, and / or one or more bacteria of ATCC deposit number PTA-127843, and / or one or more bacteria of ATCC deposit number PTA-127844, and / or one or more bacteria of ATCC deposit number PTA-127845, and / or one or more bacteria of ATCC deposit number PTA-127846, and / or one or more bacteria of ATCC deposit number PTA- 127847, and / or one or more bacteria of ATCC deposit number PTA-127848, and / or one or more bacteria of ATCC deposit number PTA-127849, and / or one or more bacteria of ATCC deposit number PTA-127850. In certain embodiments, the composition comprises one or more bacteria of the genus Microbacterium from ATCC deposit number PTA-127834 and / or the composition comprises one or more bacteria of the genus Rhodococcus from ATCC deposit number PTA- 127834 and / or the composition comprises one or more bacteria of the genus Arthrobacter from ATCC deposit number PTA-127834 and / or the composition comprises one or more bacteria of the genus Aneurinibacillus from ATCC deposit number PTA-127839 and / or the composition comprises one or more bacteria of the genus Psuedomonas from ATCC deposit number PTA- 127844 and / or the composition comprises one or more bacteria of the genus Bacillus from ATCC deposit number PTA-127847 and / or the composition comprises one or more bacteria of the genus Paenibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Pristimantibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Brevibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Paenibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the Bacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Microvirga from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Psuedomonas from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Peribacillus from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Zhihengliuella from ATCC deposit number PTA- 127849 and / or the composition comprises one or more bacteria of the genus Nonomuraea fromDocket No. OATH-015-PCT2 ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Nocardia from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Kocuria from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Actinoplanes from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Microbispora from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Mycetocola from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Nocardioides from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Priestia from ATCC deposit number PTA-127850 and / or the the composition comprises one or more bacteria of the genus Cupriavidus from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Peribacillus from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Stutzerimonas from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Caldimonas from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Terrimonas from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Neorhizobium from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Erythrobacter from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Allosphingosinicella from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127834 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127835 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127836 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127837 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127838 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127839 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127840 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127841 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127842 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127843 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127844 and / or the compositionDocket No. OATH-015-PCT2 comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127845 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127846 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127849. Details for each bacterial species and strains, and associated 16S rRNA sequences and unique designations may be found in Table 2A.
[0111] In certain embodiments, a mixture of motile and non-motile microbes is used, such as motile and non-motile microbes of the bacteria described herein. The ratio of motile to non- motile bacteria may be any suitable ratio, such as at least 0.01-10, 0.05-10, 0.1-10, 0.2-10, 0.5- 10, 0.7-10, 1-2, 1-5, or 1-10, 1-10, and / or not more than 0.05-10, 0.1-10, 0.2-10, 0.5-10, 0.7-10, 1-2, 1-5, or 1-10, 1-10, or 1-20, motile-non-motile bacteria. Fungi.
[0112] In certain embodiments, compositions provided herein comprise one or more fungal microbes. In certain embodiments, compositions provided herein comprise one or more bacterial microbes and one or more fungal microbes. A composition may comprise a composition comprising bacterial microbes as described above in this section I.B.1 and one or more fungal microbes, as described below.
[0113] Any suitable fungi may be used. In certain embodiments, provided is a composition comprising fungi of one or more of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, Sebacina, Alternaria, Beauveria, Cladiosporium, Claroideoglomus, Funneliformis, Glomus, Laccaria, Lecanicillium, Metarhizium, Piriformospora, Pisolithus, Rhizophagus, Serendipita, Talaromyces, Trichoderma, Mucor, and / or Phanerochaete genera. In certain embodiments, provided is a composition comprising fungi of one or more of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, Sebacina, Alternaria, Beauveria, Cladiosporium, Claroideoglomus, Funneliformis, Glomus, Laccaria, Lecanicillium, Metarhizium, Piriformospora, Pisolithus, Rhizophagus, Serendipita, Talaromyces, and / or Trichoderma genera. In certain embodiments, provided is a composition comprising fungi of one or more of Glomus, Rhizopogon, and / or Scleroderma genera, such as Glomus aggregatum, Glomus etunicatum, Glomus intradices, Glomaus mosseae, Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon luteolus, Rhizopogon villosullus, Scleroderma cepa, and / or Scleroderma citrinum.
[0114] In certain embodiments, provides are compositions comprising ectomycorrhizal fungi. In certain embodiments, provided is a composition comprising ectomycorrhizal fungi of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 15 differentDocket No. OATH-015-PCT2 genera, preferably 1-15, more preferably 1-10, even more preferably 1-9, yet more preferably 1-8 different genera. In certain embodiments, a composition comprises ectomycorrhizal fungi of one more genera of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, and / or Sebacina. The composition may further comprise bacteria, such as one or more of the bacteria described herein, e.g., Streptomyces and / or one or more genera comprising Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces, Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola_A, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, and / or Zhihengliuella. A composition may further comprise bacteria, such as a combinations of bacteria are as described above in this section I.B.1.
[0115] Additionally or alternatively, a composition may comprise endomycorrhizal fungi. In certain embodiments, provided is a composition comprising endomycorrhizal fungi of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 15 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, or 20 different genera, preferably 1-20, more preferably 1-17, even more preferably 1-15, yet more preferably 2-15 different genera. In certain embodiments, a composition comprises ectomycorrhizal fungi of one more genera of Alternaria, Beauveria, Cladiosporium, Claroideoglomus, Funneliformis, Glomus, Laccaria, Lecanicillium, Metarhizium, Piriformospora, Pisolithus, Rhizophagus, Serendipita, Talaromyces, and / or Trichoderma. The composition may further comprise bacteria, such as one or more of the bacteria described herein, e.g., Streptomyces and / or one or more genera comprising Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces, Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola_A, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, and / or Zhihengliuella. A composition may further comprise bacteria, such as a combinations of bacteria are as described above in this section I.B.1.
[0116] In compositions comprising fungi of the genus Aspergillus, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Aspergillus. In certain embodiments, a composition comprises Aspergillus flavus and / or Aspergillus niger.
[0117] In compositions comprising fungi of the genus Chaetomium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / orDocket No. OATH-015-PCT2 not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Chaetomium.
[0118] In compositions comprising fungi of the genus Coniothyrium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Coniothyrium. In certain embodiments, a composition comprises Coniothyrium minitans.
[0119] In compositions comprising fungi of the genus Lecanicillium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Lecanicillium.
[0120] In compositions comprising fungi of the genus Paecilomyces, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Paecilomyces. In certain embodiments, a composition comprises Paecilomyces lilacinus.
[0121] In compositions comprising fungi of the genus Penicillium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Penicillium. In certain embodiments, a composition comprises Penicillium bilaiae, Penicillium bilaii, and / or Penicillium oxalicum.
[0122] In compositions comprising fungi of the genus Phlebia, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Phlebia. In certain embodiments, a composition comprises Phlebia gigantea.
[0123] In compositions comprising fungi of the genus Rhizopogon, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Rhizopogon. In certain embodiments, a composition comprises Rhizopogon amylopogon, Rhizopogon fulvigleba, Rhizopogon luteolus, and / or Rhizopogon villosullus.
[0124] In compositions comprising fungi of the genus Scleroderma, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, suchDocket No. OATH-015-PCT2 as 1, or 2, different species of Scleroderma. In certain embodiments, a composition comprises Scleroderma cepa and / or Scleroderma citrinum.
[0125] In compositions comprising fungi of the genus Sebacina, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Sebacina. In certain embodiments, a composition comprises Sebacina vermifera.
[0126] In compositions comprising fungi of the genus Alternaria, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Alternaria. In certain embodiments, a composition comprises Alternaria alternata.
[0127] In compositions comprising fungi of the genus Beauveria, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Beauveria. In certain embodiments, a composition comprises Beauveria bassiana.
[0128] In compositions comprising fungi of the genus Cladiosporium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Cladiosporium. In certain embodiments, a composition comprises Cladiosporium tenuissimum.
[0129] In compositions comprising fungi of the genus Claroideoglomus, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Claroideoglomus. In certain embodiments, a composition comprises Claroideoglomus etunicatum.
[0130] In compositions comprising fungi of the genus Funneliformis, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Funneliformis. In certain embodiments, a composition comprises Funneliformis mosseae.
[0131] In compositions comprising fungi of the genus Glomus, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, suchDocket No. OATH-015-PCT2 as 1, or 2, different species of Glomus. In certain embodiments, a composition comprises Glomus aggregatum, Glomus etunicatum, Glomus intraradices, and / or Glomus mosseae.
[0132] In compositions comprising fungi of the genus Laccaria, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Laccaria. In certain embodiments, a composition comprises Laccaria laccata.
[0133] In compositions comprising fungi of the genus Lecanicillium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Lecanicillium.
[0134] In compositions comprising fungi of the genus Metarhizium, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Metarhizium. In certain embodiments, a composition comprises Metarhizium anisopliae.
[0135] In compositions comprising fungi of the genus Piriformospora, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Piriformospora.
[0136] In compositions comprising fungi of the genus Pisolithus, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Pisolithus. In certain embodiments, a composition comprises Pisolithus tinctorius.
[0137] In compositions comprising fungi of the genus Rhizophagus, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Rhizophagus. In certain embodiments, a composition comprises Rhizophagus intraradices and / or Rhizophagus irregularis.
[0138] In compositions comprising fungi of the genus Serendipita, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Serendipita. In certain embodiments, a composition comprises Serendipita indica.Docket No. OATH-015-PCT2
[0139] In compositions comprising fungi of the genus Talaromyces, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Talaromyces. In certain embodiments, a composition comprises Talaromyces flavus.
[0140] In compositions comprising fungi of the genus Trichoderma, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Trichoderma. In certain embodiments, a composition comprises Trichoderma asperellum, Trichoderma atrobrunneum, Trichoderma atroviride, Trichoderma harzianum, and / or Trichoderma viride.
[0141] In compositions comprising fungi of the genus Phanerochaete, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1- 3, such as 1, or 2, different species of Phanerochaete. In certain embodiments, a composition comprises Phanerochaete chrysosporium.
[0142] In compositions comprising fungi of the genus Mucor, any suitable species may be used. In certain embodiments, a composition comprises fungi of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6, preferably 1-6, more preferably 1-4, still more preferably 1-3, such as 1, or 2, different species of Mucor.
[0143] In certain embodiments, compositions and / or methods include one or more microbes deposited as ATCC Nos. PTA-127367 to PTA-127411, also referred to as one or more microbes of Designation 1-45, respectively, as shown in Table 2, and / or one or more microbes of deposits designation OATH-1 to OATH-140. Deposits may be referred to herein by ATCC number, by numerical designation (e.g., ATCC-1, corresponding to Designation 1, PTA-127367, etc.). Unless indicated otherwise, a composition said to comprises an ATCC or OATH designation, includes one or more microbes of the ATCC or OATH deposit, and progeny and / or mutants thereof. Any suitable combination of the one or more microbes deposited as ATCC Nos. PTA- 127367 to PTA-127411, also referred to as Designation 1-45, respectively, as shown in Table 2, may be used. In certain embodiments, the composition one or more microbes of Designation 1 (ATCC No. PTA-127367) and / or one or more microbes of Designation 2 (ATCC No. PTA- 127368) and / or one or more microbes of Designation 3 (ATCC No. PTA-127369) and / or one or more microbes of Designation 4 (ATCC No. PTA-127370) and / or one or more microbes of Designation 5 (ATCC No. PTA-127371) and / or one or more microbes of Designation 6 (ATCC No. PTA-127372) and / or one or more microbes of Designation 7 (ATCC No. PTA-127373)Docket No. OATH-015-PCT2 and / or one or more microbes of Designation 8 (ATCC No. PTA-127374) and / or one or more microbes of Designation 9 (ATCC No. PTA-127375) and / or one or more microbes of Designation 10 (ATCC No. PTA-127376) and / or one or more microbes of Designation 11 (ATCC No. PTA-127377) and / or one or more microbes of Designation 12 (ATCC No. PTA- 127378) and / or one or more microbes of Designation 13 (ATCC No. PTA-127379) and / or one or more microbes of Designation 14 (ATCC No. PTA-127380) and / or one or more microbes of Designation 15 (ATCC No. PTA-127381) and / or one or more microbes of Designation 16 (ATCC No. PTA-127382) and / or one or more microbes of Designation 17 (ATCC No. PTA- 127383) and / or one or more microbes of Designation 18 (ATCC No. PTA-127384) and / or one or more microbes of Designation 19 (ATCC No. PTA-127385) and / or one or more microbes of Designation 20 (ATCC No. PTA-127386) and / or one or more microbes of Designation 21 (ATCC No. PTA-127387) and / or one or more microbes of Designation 22 (ATCC No. PTA- 127388) and / or one or more microbes of Designation 23 (ATCC No. PTA-127389) and / or one or more microbes of Designation 24 (ATCC No. PTA-127390) and / or one or more microbes of Designation 25 (ATCC No. PTA-127391) and / or one or more microbes of Designation 26 (ATCC No. PTA-127392) and / or one or more microbes of Designation 27 (ATCC No. PTA- 127393) and / or one or more microbes of Designation 28 (ATCC No. PTA-127394) and / or one or more microbes of Designation 29 (ATCC No. PTA-127395) and / or one or more microbes of Designation 30 (ATCC No. PTA-127396) and / or one or more microbes of Designation 31 (ATCC No. PTA-127397) and / or one or more microbes of Designation 32 (ATCC No. PTA- 127398) and / or one or more microbes of Designation 33 (ATCC No. PTA-127399) and / or one or more microbes of Designation 34 (ATCC No. PTA-127400) and / or one or more microbes of Designation 35 (ATCC No. PTA-127401) and / or one or more microbes of Designation 36 (ATCC No. PTA-127402) and / or one or more microbes of Designation 37 (ATCC No. PTA- 127403) and / or one or more microbes of Designation 38 (ATCC No. PTA-127404) and / or one or more microbes of Designation 39 (ATCC No. PTA-127405) and / or one or more microbes of Designation 40 (ATCC No. PTA-127406) and / or one or more microbes of Designation 41 (ATCC No. PTA-127407) and / or one or more microbes of Designation 42 (ATCC No. PTA- 127408) and / or one or more microbes of Designation 43 (ATCC No. PTA-127409) and / or one or more microbes of Designation 44 (ATCC No. PTA-127410) and / or one or more microbes of Designation 45 (ATCC No. PTA-127411). In certain embodiments, a composition can include one or more microbes from one or more microbes of Designation 46 (ATCC No. PTA-127412), also designated individually as O-46 to O-140 herein. In certain embodiments, the composition comprises one or more microbes of Designation O-46 and / or one or more microbes of Designation O-47 and / or one or more microbes of Designation O-48 and / or one or moreDocket No. OATH-015-PCT2 microbes of Designation O-49 and / or one or more microbes of Designation O-50 and / or one or more microbes of Designation O-51 and / or one or more microbes of Designation O-52 and / or one or more microbes of Designation O-53 and / or one or more microbes of Designation O-54 and / or one or more microbes of Designation O-55 and / or one or more microbes of Designation O-56 and / or one or more microbes of Designation O-57 and / or one or more microbes of Designation O-58 and / or one or more microbes of Designation O-59 and / or one or more microbes of Designation O-60 and / or one or more microbes of Designation O-61 and / or one or more microbes of Designation O-62 and / or one or more microbes of Designation O-63 and / or one or more microbes of Designation O-64 and / or one or more microbes of Designation O-65 and / or one or more microbes of Designation O-66 and / or one or more microbes of Designation O-67 and / or one or more microbes of Designation O-68 and / or one or more microbes of Designation O-69 and / or one or more microbes of Designation O-70 and / or one or more microbes of Designation O-71 and / or one or more microbes of Designation O-72 and / or one or more microbes of Designation O-73 and / or one or more microbes of Designation O-74 and / or one or more microbes of Designation O-75 and / or one or more microbes of Designation O-76 and / or one or more microbes of Designation O-77 and / or one or more microbes of Designation O-78 and / or one or more microbes of Designation O-79 and / or one or more microbes of Designation O-80 and / or one or more microbes of Designation O-81 and / or one or more microbes of Designation O-82 and / or one or more microbes of Designation O-83 and / or one or more microbes of Designation O-84 and / or one or more microbes of Designation O-85 and / or one or more microbes of Designation O-86 and / or one or more microbes of Designation O-87 and / or one or more microbes of Designation O-88 and / or one or more microbes of Designation O-89 and / or one or more microbes of Designation O-90 and / or one or more microbes of Designation O-91 and / or one or more microbes of Designation O-92 and / or one or more microbes of Designation O-93 and / or one or more microbes of Designation O-94 and / or one or more microbes of Designation O-95 and / or one or more microbes of Designation O-96 and / or one or more microbes of Designation O-97 and / or one or more microbes of Designation O-98 and / or one or more microbes of Designation O-99 and / or one or more microbes of Designation O-100 and / or one or more microbes of Designation O-101 and / or one or more microbes of Designation O-102 and / or one or more microbes of Designation O-103 and / or one or more microbes of Designation O-104 and / or one or more microbes of Designation O-105 and / or one or more microbes of Designation O-106 and / or one or more microbes of Designation O-107 and / or one or more microbes of Designation O-108 and / or one or more microbes of Designation O-109 and / or one or more microbes of Designation O-110 and / or one or more microbes of Designation O-111 and / or one or more microbes of Designation O-112 and / or one or more microbes ofDocket No. OATH-015-PCT2 Designation O-113 and / or one or more microbes of Designation O-114 and / or one or more microbes of Designation O-115 and / or one or more microbes of Designation O-116 and / or one or more microbes of Designation O-117 and / or one or more microbes of Designation O-118 and / or one or more microbes of Designation O-119 and / or one or more microbes of Designation O-120 and / or one or more microbes of Designation O-121 and / or one or more microbes of Designation O-122 and / or one or more microbes of Designation O-123 and / or one or more microbes of Designation O-124 and / or one or more microbes of Designation O-125 and / or one or more microbes of Designation O-126 and / or one or more microbes of Designation O-127 and / or one or more microbes of Designation O-128 and / or one or more microbes of Designation O-129 and / or one or more microbes of Designation O-130 and / or one or more microbes of Designation O-131 and / or one or more microbes of Designation O-132 and / or one or more microbes of Designation O-133 and / or one or more microbes of Designation O-134 and / or one or more microbes of Designation O-135 and / or one or more microbes of Designation O-136 and / or one or more microbes of Designation O-137 and / or one or more microbes of Designation O-138 and / or one or more microbes of Designation O-139 and / or one or more microbes of Designation O-140. In certain embodiments, a composition comprises at least 5, 10, 15, 20, 25, 30, 35, or 40 of one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA-127411) and / or not more than 5, 10, 15, 20, 25, 30, 35, or 40 of one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA-127411), preferably 5-45, more preferably 10-45, even more preferably 20- 45, yet more preferably 35-45. In certain embodiments, a composition comprises at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, or 135 different microbes and / or not more than 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135 or 140 of one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA-127411) and / or one or more microbes of Designation O-46 to O-140, for example at least 20, in certain cases at least 50, in yet other cases at least 70, or preferably 10-140, more preferably 20-120, even more preferably 40-100, yet more preferably 50-100.
[0144] In certain embodiments, microbes included in a composition herein are biologically pure. As used herein, a "pure" or “biologically pure” organism or population of organisms, such as microbe or microbial population, or the like, includes a population of organisms in the absence of other species or types. A "substantially pure culture" includes a culture which contains substantially no other microbes than the desired strain or strains, e.g., other microbes may be present but not in quantities and / or forms that interfere with the intended use of the culture. A substantially pure culture includes a culture that is substantially free of other contaminants, which can include microbial contaminants as well as undesirable chemical contaminants.Docket No. OATH-015-PCT2
[0145] In certain embodiments, microbes included in a composition herein are isolated. As used herein, "isolate," "isolated," "isolated microbe," “isolated microorganism” and like terms, include the meaning that the one or more microbes has been separated from at least one of the materials with which it is associated in a particular environment (for example soil, water, plant tissue). Thus, an "isolated microbe" does not exist in its naturally occurring environment; rather, the microbe has been removed from its natural setting and placed into a non-naturally occurring state of existence.
[0146] In certain embodiments, microbes of a composition or method are heterologous to each other, and / or heterologous to a soil, plant, plant part, seed, seedling, plant propagule, or other substance in association with the microbes; in certain embodiments at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 30, 40, 50, 60, 70, 80, 90 or 100 and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 17, 20, 30, 40, 50, 60, 70, 80, 90100, 120, or 140, preferably 2-140, more preferably 2-100, even more preferably 2-80, still more preferably 2-60, yet more preferably 2-40 for example at least 2, preferably at least 3, more preferably at least 4, even more preferably at least 5, yet more preferably at least 7, still more preferably at least 10 microbes of a composition or method are heterologous to each other, or heterologous to a soil, plant, plant part, seed, seedling, plant propagule, or other substance in association with the microbes. As used herein, the terms “heterologous,” "exogenous,” and the like, when referring to one material related to a second material, include a material that is not naturally associated with the second material. Thus, a microbe is considered heterologous to a plant or a plant part, such as a root, or a seed, or the like, if the microbe is not found in nature associated with the particular plant, plant part (e.g., root), seed, or the like, or is not found in the number indicated; in some cases, a population of microbes may contain some microbes that are heterologous to the material, and some that are not heterologous (i.e., normally found with the material) to the material. A microbe can also be heterologous to another microbe if the two are not naturally found together, or are not found in the numbers and / or ratios indicated.
[0147] In certain embodiments, total colony forming units (CFUs) per gram of a microbial composition is at least 104, 105,106, 107, 108, 109, 1010, 1011, 1012, 1013, or 1014and / or not more than 105,106, 107, 108, 109, 1010, 1011, 1012, 1013, 1014, 1015, or 1016, preferably 104to 1011, more preferably 104to 1011even more preferably 105to 1011, yet more preferably 108to 1011, still more preferably 109to 1011. In certain embodiments a composition comprises at least 108CFU / gm. In certain embodiments a composition comprises at least 109CFU / gm. In certain embodiments a composition comprises at least 109CFU / gm. In certain embodiments a composition comprises at least 1010CFU / gm. In certain embodiments, each of the different microbes (e.g., microbes of a specific strain) is present at at least 104, 105,106, 107, 108, 109, 1010,Docket No. OATH-015-PCT2 1011, 1012, 1013, or 1014, and / or not more than 105,107, 108, 109, 1010, 1011, 1012, 1013, 1014, or 1015CFU / gm of composition, preferably 106to 1015, more preferably 107to 1014, even more preferably 107to 1013, yet more preferably 107to 1011, even yet more preferably 108to 1011CFU / gm of composition. In certain embodiments at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 95%, or 100%, preferably at least 10%, more preferably at least 20%, even more preferably at least 30%, still more preferably at least 40%, yet more preferably at least 50% of different strains of microbes in composition are present in a ratio to each other (CFU to CFU) of 20:1 to 1:20, preferably 10:1 to 1:10, more preferably 5:1 to 1:5, even more preferably 2:1 to 1:2, yet more preferably 1.5:1 to 1:1.5, even more preferably 1.25:1 to 1:1.25. In certain embodiments at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 95%, or 100%, preferably at least 10%, more preferably at least 20%, even more preferably at least 30%, still more preferably at least 40%, yet more preferably at least 50% of different strains of microbes in composition are present in a weight ratio to each other of 20:1 to 1:20, preferably 10:1 to 1:10, more preferably 5:1 to 1:5, even more preferably 2:1 to 1:2, yet more preferably 1.5:1 to 1:1.5, even more preferably 1.25:1 to 1:1.25. A “colony forming unit,” or “CFU,” as those terms are used herein, includes a microbial cell that is viable, that is, able to multiply via binary fission under suitable conditions. Methods for determining a number of CFUs in a sample are well-known in the art.
[0148] In certain embodiments, a composition comprises a liquid medium in which the one or more microbes, e.g., as described above, are distributed. In certain embodiments the liquid medium is heterologous to microorganisms in the composition. In certain embodiments, the liquid medium comprises an aqueous medium. In certain embodiments, the aqueous medium comprises a combination of mediums in which one or more of the one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA-127411) and / or one or more microbes of Designation O-46 to O-140 and / or one or more microbes of ATCC Nos.127834-127850 has been growing. 2. Dry compositions
[0149] In certain embodiments a composition, such as a composition comprising one of the compositions comprising bacteria and / or fungi, e.g., a composition of section I.B.1, is a dry composition. Generally, a dry composition comprises 10 wt% or less water. In certain embodiments a composition comprises less than 5, 4, 3, 2, 1, 0.5, 0.2, 0.1%, 0.05%, 0.01%, 0.005%, 0.001%, 0.0005%, or 0.0001% wt% water, for example less than 0.1%, preferably less than 0.05%, more preferably less than 0.01%, even more preferably less than 0.005%, yet more preferably less than 0.001%, still more preferably less than 0.0005%. In certain embodiments, aDocket No. OATH-015-PCT2 composition comprises less than 5, such as less than 4, or less than 3, or less than 2% water. In certain embodiments, a composition comprises less than 1% water. In certain embodiments, a composition comprises less than 0.5% water. In certain such embodiments, a composition consists essentially of the one or more microbes, e.g., a composition produced from a combination of different liquid media comprising the microbes, in some cases, media in which the microbes have been grown, that is then treated to obtain the desired dryness, and which may include one or more components from the media, and / or other components, that do not materially affect the basic and novel characteristics the composition. 3. Particulate compositions.
[0150] In certain embodiments, provided herein are compositions comprising particles, such as particles comprising one of the microbial compositions provided herein. As used herein a “particle,” or “particles” include discrete objects larger than atoms or molecules; a particle can be large enough to be seen with the naked eye, or microscopic. “Particle size” includes size as determined by methods used in the art, in particular, sieving.
[0151] In certain embodiments, provided herein are compositions comprising a plurality of particles wherein each particle comprises a composition comprising one or more microbes, e.g., one or more bacteria and / or one or more fungi, as described herein. The particles of the composition may be any suitable size; in some cases particles are all within a certain size range, in other cases particles may be present in two or more populations, each of which is within a different size range. In certain embodiments the particles have a median particle size of at least 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 220, 250, 370, 300, or 400 um and / or no more than 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 220, 250, 270, 300, 400, or 500 um, for example 20-500 um, preferably 90-270 um, more preferably 140-220 um, even more preferably 160-200 um, still more preferably 170-190 um. In certain embodiments, at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 95, 97, 98, or 99% and / or not more than 20, 30, 40, 50, 60, 70, 80, 90, 95, 97, 98, 99, 99.5, or 100%, or 100%, for example 10-100%, preferably 20-100%, more preferably 50-100%, even more preferably 80-100%, still more preferably 90-100% of the particles have a mean particle size of at least 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 220, 250, 370, 300, or 400 um and / or no more than 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 220, 250, 270, 300, 400, or 500 um, for example 20-500 um, preferably 90-270 um, more preferably 140-220 um, even more preferably 160-200 um, still more preferably 170-190 um.Docket No. OATH-015-PCT2
[0152] In certain embodiments, such as embodiments where the composition comprises at least 60, 70, 80, 90, 92, 95, 97, 98, 99, or 99.5%, or 100%, microbes (wt%), particles may have a mean or median particle size of at least 0.1, 0.2, 0.5, 0.7, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, or 20 um and / or not more 0.2, 0.5, 0.7, 1, 1.5, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, 20, 30, or 40 um, preferably 0.2-40 um, more preferably 0.5-20 um, even more preferably 1-20 um, still more preferably 1-15 um, yet more preferably 2-15 um, even still more preferably 2-12 um.
[0153] In certain embodiments, larger particles may be used, e.g., in compositions for aerial application and / or some delayed release compositions larger particles may be used, for example, with a mean size, or a median size, of at least 100, 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 3000, 4000, 5000, or 7000 um and / or no more than 150, 200, 250, 300, 400, 500, 600, 700, 800, 900, 1000, 1500, 2000, 3000, 4000, 5000, 7000, or 10,000 um, or even larger, such as 200-10,000, preferably 500-5000, more preferably 1000-10,000 um. Such larger particulate compositions can be useful, for example to allow for release of microbes in two or more batches as the particles are subject to conditions that allow release of microbes from the surface of the particles but others are retained in the particle until the conditions occur again (e.g., rainfall, irrigation, or the like, see below). It will be understood that for any composition in which a plurality of populations of particles is used, with different particle sizes, any suitable sizes may be used for the composition.
[0154] Certain particulate compositions are dry compositions. Certain particulate compositions consist essentially of a microbial composition. Certain particulate compositions comprise a microbial composition that includes one or more agricultural adjuvants. 4. Delayed release compositions
[0155] In certain embodiments, delayed release compositions are provided. A “delayed release” composition, as that term is used herein, includes a composition that does not release at least a portion of its active ingredients, e.g., microbes, on first exposure to the environment, or to an environmental trigger in the environment in which it will be used; in certain embodiments, a delayed release composition can release a portion of its active ingredients, e.g., microbes, on first exposure, so long as an additional portion is not released, while in other embodiments none of the active ingredients, e.g., microbes, is released on first exposure. Any suitable materials and configuration may be used, so long as not all of the microbes are released on first exposure to the environment of use or environmental trigger. Materials useful in providing delayed release compositions are well-known in the art; while many of these are useful in pharmaceutical preparations, suitable modifications, if necessary, will be readily apparent. In general materials and / or configurations of such compositions are such that microbes are confined to theDocket No. OATH-015-PCT2 composition during normal conditions of manufacture and transport, but that release at least a portion of the microbes on exposure to one or more environmental triggers, that is, one or more conditions found in the environment of use. Any suitable trigger may be used, such as water, electromagnetic radiation (e.g., sunlight, or certain wavelengths of sunlight), temperature, or even air (e.g., if particles are relatively unexposed to air during transport and storage, for example in an airtight bag, then start to degrade on exposure to air, e.g., when placed in their environment of use), or a combination thereof. In certain embodiments, an environmental trigger is water, which can come from, e.g., rainfall, irrigation, or the like. In general, “exposure to” the environmental trigger or trigger includes sufficient exposure of the composition that the process that occurs to release microbes can occur.
[0156] In certain embodiments, provided is a delayed release composition comprising a plurality of particles, each of which comprises a composition comprising microbes, e.g., one or more bacteria and / or one or more fungi, as described herein, wherein the plurality of particles comprises a first population of particles wherein each particle of the first population comprises one or more first components configured to confine at least a portion of the microbes to the particles, and wherein the one or more first components are such that the one or more first components lose sufficient integrity upon exposure to one or more environmental triggers that at least a portion of the confined microbes are released from the particles. In certain embodiments, the composition comprises a microbial composition as provided herein. In certain embodiments the first components are configured to confine at least 50, 60, 70, 80, 90, 95, 96, 97, 98, or 99% of the microbes, for example at least 60%, preferably at least 80%, more preferably at least 90%, even more preferably at least 95%. Any suitable configuration of the first components may be used. In certain embodiments the first components form a shell wherein the microbes are confined within, and / or embedded in, and / or associated with a surface of, the shell. In certain embodiments the one or more first components form a matrix in which the microbes are embedded or otherwise associated. In certain embodiments the one or more first components form a plurality of lamellae, wherein the particles are confined between, and / or embedded in, and / or associate with a surface of, the lamellae. It will be appreciated that any or all of these may be combined in particles. In certain embodiments the environmental trigger comprises water, air, temperature, electromagnetic radiation, or a combination thereof. In certain embodiments the environmental trigger is water, for example, water from rainfall, irrigation, or even humidity of the air. In certain embodiments, exposure to the trigger is sufficient to release all the microbes; in other embodiments, repeated exposures and / or duration of exposure is required. Thus, for example, a composition may be formulated so that it remains intact until a first rainfall, when most or all of the particles in the composition are exposed to water. Components andDocket No. OATH-015-PCT2 configuration can be such that all microbe are released at the first rainfall; alternatively, components and configuration can be such that repeated rainfalls, or rainfall of sufficient duration to keep exposing the compositions to water for a prolonged period, is required to release all the microbes. In certain embodiments one or more first components and / or structure of the particles are such that exposure to the trigger causes sufficient loss of integrity that at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 97, 98, or 99% and / or not more than 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 97, 98, 99, or 100% of the microbes are released. In certain embodiments 30-100% of the microbes are released. In certain embodiments 50-100% of the microbes are released. In certain embodiments n 80-100% of the microbes are released. In certain embodiments 90-100% of the microbes are released. In certain embodiments 5-80% of the microbes are released. In certain embodiments 5-60% of the microbes are released. In certain embodiments 5-40% of the microbes are released. In certain embodiments 10-40% of the microbes are released. In certain embodiments 5-30% of the microbes are released. In certain embodiments 10-30% of the microbes are released. In certain embodiments 5-25% of the microbes are released. In certain embodiments 10-25% of the microbes are released. In certain embodiments a composition further comprises a second population of particles wherein each particle of the second population comprises one or more second components configured to confine at least a portion of the microbes to the particles, and wherein the one or more second components are such that the one or more second components withstand exposure to the one or more environmental triggers that cause the first components of the first population to lose integrity, but that lose sufficient integrity upon further exposure to the one or more environmental triggers that at least a portion of the confined microbes are released from the particles of the second population. In certain embodiments an additional 5-80% of the microbes are released. In certain embodiments an additional 5-60% of the microbes are released. In certain embodiments an additional 5-40% of the microbes are released. In certain embodiments an additional 10-40% of the microbes are released. In certain embodiments an additional 5-30% of the microbes are released. In certain embodiments an additional 10-30% of the microbes are released. In certain embodiments an additional 5-25% of the microbes are released. In certain embodiments an additional 10-25% of the microbes are released. In certain embodiments a composition comprises a third population of particles wherein each particle of the third population comprises one or more third components configured to confine at least a portion of the microbes to the particles, and wherein the one or more third components are such that the one or more third components withstand exposure to the one or more environmental triggers that cause the first and second components of the first population and second populations to lose integrity, but that lose sufficient integrity upon further exposure to the one or moreDocket No. OATH-015-PCT2 environmental triggers that at least a portion of the confined microbes are released from the particles of the third population. In certain embodiments an additional 5-80% of the microbes are released. In certain embodiments an additional 5-60% of the microbes are released. In certain embodiments an additional 5-40% of the microbes are released. In certain embodiments an additional 10-40% of the microbes are released. In certain embodiments an additional 5-30% of the microbes are released. In certain embodiments an additional 10-30% of the microbes are released. In certain embodiments an additional 5-25% of the microbes are released. In certain embodiments an additional 10-25% of the microbes are released. In certain embodiments a composition further comprises a fourth population of particles wherein each particle of the fourth population comprises one or more fourth components configured to confine at least a portion of the microbes to the particles, and wherein the one or more fourth components are such that the one or more fourth components withstand exposure to the one or more environmental triggers that cause the first, second and third components of the first population, second and third populations to lose integrity, but that lose sufficient integrity upon further exposure to the one or more environmental triggers that at least a portion of the confined microbes are released from the particles of the fourth population. In certain embodiments an additional 5-80% of the microbes are released. In certain embodiments an additional 5-60% of the microbes are released. In certain embodiments an additional 5-40% of the microbes are released. In certain embodiments an additional 10-40% of the microbes are released. In certain embodiments an additional 5-30% of the microbes are released. In certain embodiments an additional 10-30% of the microbes are released. In certain embodiments an additional 5-25% of the microbes are released. In certain embodiments an additional 10-25% of the microbes are released. 5. Compositions comprising one or more agriculturally acceptable adjuvants.
[0157] Provided herein are compositions comprising microbes, such as a composition as described above, e.g., one or more bacteria and / or one or more fungi, as described herein, and one or more agriculturally acceptable adjuvants. An “agriculturally acceptable adjuvant,” as that term is used herein, includes a natural or synthetic, organic or inorganic material with which the active compounds (e.g. the one or more microbes as disclosed herein) are combined, e.g., to facilitate their manufacture, storage, use, or the like, where the substance can be used under the desired conditions, e.g., conditions in which plants grow or are maintained, or are to be grown or maintained. Thus, in certain embodiments provided herein is a composition comprising any one of the compositions comprising one or more microbes as described above and further comprising one or more agriculturally acceptable adjuvants. It will be appreciated that in certain cases, such as delayed release compositions comprising one or more components to confine microbes and toDocket No. OATH-015-PCT2 release them on exposure to one or more environmental triggers, certain of the components may already be considered as agriculturally acceptable adjuvants; in such cases, one or more additional components, also considered agriculturally acceptable adjuvants, may be used.
[0158] Any suitable adjuvant may be used. In certain embodiments at least one of the agriculturally acceptable adjuvants is heterologous to the one or more microbes of the composition. In certain embodiments the one or more agriculturally acceptable adjuvants comprise one or more liquid carriers, solid carriers, surfactants, viscosity modifiers, thickeners, rheology additives, structuring agents, preservatives, biocides or biostatic agents, antifreezes, crystallization inhibitors, suspending agents, dyes, anti-oxidants, foaming agents, light absorbers, light blockers, mixing auxiliaries, antifoams, complexing agents, neutralizing or pH-modifying substances and buffers, corrosion inhibitors, fragrances, wetting agents, take-up enhancers, micronutrients, plasticizers, glidants, lubricants, and dispersants.an emulsifier, a carrier, a stabilizer, or a combination thereof. In certain embodiments, the one or more agriculturally acceptable adjuvants comprise one or more surfactants. Any suitable surfactant may be used. Generally, it is desired that a surfactant facilitates dissolution of a component or formation of an emulsion with a component, and a suitable surfactant will do so, while not affecting viability of the one or more microbes, or not affect viability to a sufficient degree to interfere with the intended use of the composition. Surfactants belong to different classes such as cationic surfactants, anionic surfactants, non-ionic surfactants, ionic surfactants, and amphoteric surfactants. A surfactant can be any surfactant or a combination of two or more surfactants useful to disperse the microbes and / or other components in the composition or tank mix for application. Any suitable concentration of surfactant can be used, such as at least 0.1, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, or 15% and / or not more than 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 15, or 20% w / w, for example 0.5 to 20%, preferably 1 to 10%, more preferably 2 to 8%, even more preferably 3 to 8%, yet more preferably 5 to 7%. Non-ionic surfactants suitable for this invention include ethoxylated linear alcohols, ethoxylated alkyl phenol, alkyl EO / PO copolymer, polyalkylene glycol monobutyl ether ethoxylated fatty acids / oils, sorbitan laurate, polysorbate, sorbitan oleate, ethoxylated fatty acid alcohols, or alkyl phenols, alkanolamides or alkyloamides (such as diethanolamide, lauric acid monoisopropanolamide, and ethoxylated myristamide), xyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers (such as alkylaryl polyglycol ethers), alkylphenol / alkylene oxide addition products, such as nonylphenol ethoxylate; alcohol / alkylene oxide addition products, such as tridecylalcohol ethoxylate. Anionic surfactants include alkyl-, alkylaryl- and arylsulfonates or salts thereof (such as sodium, potassium or calcium salts of lauryl sarcosinate, alkylbenzenesulfonate, dodecylbenzenesulfonate, alkylnaphthalenesulfonates such as dibutylnaphthalenesulfonate, or C.sub.14-16 olefin sulfonates), alkyl-, alkylaryl- andDocket No. OATH-015-PCT2 arylsulfates or salts thereof (such as sodium, potassium or calcium salts of tridedeth sulfate, lauryl sulfate, decyl sulfate, and diethanolammonium lauryl sulfate) protein hydrolysates, derivatives of polycarboxylic acid (such as ammonium lauryl ether carboxylate), olefin sulfonates (such as sodium alpha olefin sulfonate), sarcosinates (such as ammonium cyclohexyl palmitoyl taurinate), succinates (such as disodium N-octadecyl sulfosuccinamate), phosphorus derivatives (such as phosphoric acid esters and their equivalent salts). Cationic surfactants include alkylbenzyltrimethylammonium chloride, ammonium lauryl sulfate and lauramine oxide. Further examples of surfactants include nitrogen-surfactant blends such as Prefer 28 (Cenex), Surf-N (US), Inhance (Brandt), P-28 (Wilfarm) and Patrol (Helena); esterified seed oils including Sun-It II (AmCy), MSO (UAP), Scoil (Agsco), Hasten (Wilfarm) and Mes-100 (Drexel); and organo-silicone surfactants include Silwet L77 (UAP), Silikin (Terra), Dyne-Amic (Helena), Kinetic (Helena), Sylgard 309 (Wilbur-Ellis) and Century (Precision), polysorbate 20, polysorbate 80, Tween 20, Tween 80, Scattics, Alktest TW20, Canarcel, Peogabsorb 80, Triton X-100, Conco NI, Dowfax 9N, Igebapl CO, Makon, Neutronyx 600, Nonipol NO, Plytergent B, Renex 600, Solar NO, Sterox, Serfonic N, T-DET-N, Tergitol NP, Triton N, IGEPAL CA-630, Nonident P-40, Pluronic.
[0159] In certain embodiments the one or more surfactants comprise a non-ionic, cationic, or anionic surfactant, or a combination thereof. In certain embodiments the one or more surfactants comprise Tween 20 or Tween 80. In certain embodiments the one or more surfactants comprise soy lecithin or yucca powder or a derivative thereof, or a combination thereof. In certain embodiments the one or more surfactants are organic. In certain embodiments the one or more surfactants are at a total concentration of at least 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 3, 4, 5, 6, 7, 8, or 9 wt% and / or no more than 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 3, 4, 5, 6, 7, 8, 9, or 10 wt%, for example 0.5-10 wt%, preferably 1-3 wt%, more preferably 1.8-2.2 wt%.
[0160] In certain embodiments, a composition comprising one or more agriculturally acceptable adjuvants is a solid composition. In certain embodiments, a composition comprising one or more agriculturally acceptable adjuvants is a liquid composition. In certain embodiments, a composition comprises polyethylene glycol (PEG). In certain embodiments, a composition comprises PEG and glycerol. The composition comprising PEG may be solid or liquid, depending on the molecular weight of the PEG.
[0161] In certain embodiments, a composition the one or more adjuvants comprise one or more carriers. As used herein, the term “carrier” includes a substance with which the one or more microbes associate in such a way that the one or more microbes are carried with the substance; the association may be merely physical, e.g., a physical combination of microbes with carrier;Docket No. OATH-015-PCT2 alternatively or additionally, the association may be chemical, e.g., noncovalent, such as adsorption, or covalent. In certain embodiments in which the composition is a solid composition, one or more solid carriers may be used. Any suitable solid carrier may be used. Exemplary solid carriers include, carbohydrates including mono or disaccharides, such as glucose, mannose, lactose, sucrose; oligo or poly-saccharides such as maltodextrin or pectin, or oligo- or polysaccharides derived from one or more plant substances; talc, titanium dioxide, pyrophyllite clay, attapulgite clay, kieselguhr, silica (silicon dioxide), limestone, bentonite, calcium montmorillonite water soluble salts such as sodium, potassium, magnesium, calcium or ammonium salts of acetate, carbonate, chloride, citrate, phosphate, or sulfate such as calcium carbonate; plant parts such as cottonseed husks, wheat flour, soybean flour, wood flour, ground walnut shells, lignin and similar substances, yeast extracts, fish meal, pumice, or mixtures thereof. In certain embodiments, the carrier comprises an alcohol and / or a saccharide, e.g., inulin, glycerol and / or a monosaccharide, disaccharide, trisaccharide, or polysaccharide. In certain embodiments, the carrier comprises glucose, sucrose, lactose, maltose, and / or galactose. In certain embodiments, the carrier comprises glucose. In certain embodiments, the carrier is at a concentration in the composition of at least 50, 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, or 99 wt% and / or no more than 55, 60, 65, 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, or 100 wt%, for example 50-100 wt%, preferably 70-100 wt%, more preferably 80- 100 wt%, even more preferably 90-100 wt%, still more preferably 95-100 wt%.
[0162] In certain embodiments, a composition comprises a monosaccharide, e.g., glucose, an emulsifier (surfactant), e.g., soy lecithin, and microbes. In certain embodiments, the monosaccharide is present at at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5% and or not more than 85, 90, 95, 96, 97, 98, 99, 99.5, or 99.9%, such as 90-99%, preferably 85-99%, more preferably 90-99%, even more preferably 90-98%, yet more preferably 90-96%, still more preferably 90-94%; In certain embodiments, the emulsifier is present at at least 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 1, 1.2, 1.5, 2, 3, 4, or 5% and / or not more than 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 1, 1.2, 1.5, 2, 3, 4, 5, or 7%, such as 0.1-5%, preferably 0.1-4%, more preferably 0.1-3%, still more preferably 0.5-3%. In certain embodiments, the microbes are present at at least 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 1, 1.2, 1.5, 2, 3, 5, 6, 7, 8, 9, or 10% and / or not more than 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 1, 1.2, 1.5, 23, 5, 6, 7, 8, 9, 10, 12, or 15%, such as 0.1-15%, preferably 0.5-12%, more preferably 0.5-10%, still more preferably 1-12%, yet more preferably 1-10%, still yet more preferably 2-10%, even yet more preferably 4-8%.
[0163] In certain embodiments the one or more agriculturally acceptable adjuvants comprise one or more materials that protect the microbes from one or more aspects of an environment in which the microbes will be stored and / or used. In certain embodiments the one or moreDocket No. OATH-015-PCT2 agriculturally acceptable adjuvants comprise a light absorber or light blocker, or a combination thereof. In certain embodiments the light absorber or light blocker protects the microbes of the composition from ultraviolet (UV) light. In certain of these embodiments, the microbes are enclosed in the material, e.g., the material forms an opaque or translucent shell. Alternatively or additionally, the material can be a UV absorber, either in a shell or as a matrix or similar in which the microbes are situated. In certain embodiments the one or more materials are such that they release some or all of the microbes upon exposure to one or more environmental triggers, such as one or more environmental triggers described herein. In certain embodiments the one or more environmental triggers comprises water.
[0164] In certain embodiments, a composition comprising one or more agriculturally acceptable adjuvants is stable at temperatures between 4 ºC. and 37 ºC for at least 1, 2, 3, 4, 5, or 6 days, or 1, 2, 3 or 4 weeks, or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 or 12 months, or 1, 2, 3, 4, or 5 years, for example, stable at between 4 ºC. and 37 ºC for at least one week, preferably at least one month, more preferably at least 6 months, even more preferably at least 12 months, yet more preferably at least 2 years. “Stable,” as that term is used herein in relation to a composition, includes that the composition retains sufficient viability to be used for its intended purpose.
[0165] In certain embodiments, a composition includes an antifreeze agent for cold storage, e.g., at temperature less than -5, -10, -15, -20, -25, -30, -40, -50, -60, -70, -80 ºC, for example less than -5, preferably less than -10, more preferably less than -15, even more preferably less than -20 ºC. Such an agent can be considered an “agriculturally acceptable adjuvant,” as that term is used herein, in that it maintains the one or more microbes in a form where they are viable for their intended use, though generally after steps to grow the microbes and further treat them. In certain embodiments the antifreeze agent comprises glycerol, ethylene glycol, propylene glycol, urea, calcium chloride, sodium nitrate, magnesium chloride and ammonium sulfate. In certain embodiments the antifreeze agent comprises glycerol. In certain embodiments the glycerol is present at a concentration of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 35, 40, or 45% and / or no more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 35, 40, 45, or 50%, for example 1-50%, preferably 10-30%, more preferably 15- 25%, even more preferably 18-22% wt%. In certain of these embodiments the composition comprises microbes of only one strain.
[0166] Certain embodiments provide a plurality of particles comprising a composition comprising one or more agriculturally acceptable adjuvants, as described herein, and a composition comprising microbes, such as one of the microbial compositions herein.
[0167] Docket No. OATH-015-PCT2 6. Compositions comprising additional microbes.
[0168] In certain embodiments provided are methods and compositions utilizing any one of the compositions described above, and further including additional microbes. In certain embodiments provided is a composition comprising one or more microbes as described above and further comprising cells of one or more additional microbial species or strains different from those of one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA-127411) and / or one or more microbes of Designations O-46 to O-140. In certain embodiments the additional microbial species comprise fungi, bacteria, or a combination thereof. In certain embodiments the additional microbial species comprise fungi. In certain embodiments the fungi comprise mycorrhizal fungi. 7. Seeds, seedlings, or other plant propagules and one or more microbes
[0169] In certain embodiments, provided are seeds, seedlings, or other plant propagules comprising microbes, for example of one of the microbial compositions described herein, including but not limited to in sections I.B.1-6, for example section I.B.1, or I.B.5, or I.B.6. A “plant propagule,” as that term is used herein, includes any plant material used for the purpose of plant propagation, including but not limited to seeds, seedlings, and stem cuttings. In certain embodiments provided is a population of seeds, seedlings, or other plant propagules comprising a composition comprising a plurality of microbes, such as described above, including but not limited to in sections I.B.1-6, for example section I.B.1, or I.B.5, or I.B.6, wherein at least a portion of the microbes of the composition are associated with the seeds, seedlings, or other plant propagules. It will be appreciated that in certain cases, e.g., where a large number of different microbes are used, individual seeds, seedlings, or plant propagules may not necessarily comprise every one of the different microbes; this is acceptable so long as the overall population comprises a sufficient number of the different microbes, preferably all of them, for the microbes to operate for the desired purpose. Thus, in certain embodiments at least 50, 60, 70, 80, 85, 90, 95, 97, or 99%, or 100%, of the seeds, seedlings, or other plant propagules of the population, for example at least 50%, preferably at least 60%, more preferably at least 80%, yet more preferably at least 85%, and even yet more preferably at least 90%, each has at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, or 99%, or 100%, for example at least 10%, preferably at least 20%, more preferably at least 30%, even more preferably at least 40%, yet more preferably at least 60%, still more preferably at least 80% of the different microbes of one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA-127411) and / or one or more microbes of Designations O-46 to O-140 associated with it, for example at least 10, preferably at least 20, more preferably at least 30, even more preferably at least 40, yet more preferably at least 50%. In certainDocket No. OATH-015-PCT2 embodiments the microbes are present on each seed, seedling, or other plant propagule in an average total amount of at least 10, 100, 200, 500, 700, 1000, 1500, 2000, 3000, 5000, 7000, 10,000, 20,000, or 50,000 and / or not more than 100, 200, 500, 700, 1000, 1500, 2000, 3000, 5000, 7000, 10,000, 20,000, 50,000, 100,000, 500,000, or 1M CFU per seed, seedling, or other plant propagule, for example, 10-1M, preferably 100-500,000, more preferably 500-500,000, even more preferably 1000-500,000, yet more preferably 5000-500,000, yet more preferably 5000-1M. In certain embodiments the population of seeds, seedlings, or other plant propagules comprises one or more microbes of Designations 1-45 (ATCC Nos. PTA-127367 to PTA- 127411) and / or one or more microbes of Designations O-46 to O-140. In certain embodiments the microbes of the composition are disposed on surfaces, in pores, or a combination thereof, of the seeds, seedlings, or other plant propagules. In certain embodiments a composition comprises at least 100, 500, 1000, 5000, 10,000, 50,000, 100,000, or 1M seeds, seedlings, other plant propagules, for example at least 1000, preferably at least 10,000, more preferably at least 100,000. In certain embodiments the composition is configured to be suitable for aerial deposition, e.g., aerial deposition of seeds, seedlings, or other plant propagules comprising microbes as described herein, so that a desired proportion of the deposited seeds, seedlings, or other plant propagules are situated so that they can germinate and grow under suitable conditions. In certain embodiments a composition comprises seeds, wherein the seeds are contained in a bag or container suitable for commercial sale. In certain embodiments at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, or 99%, or 100%, for example at least 10%, preferably at least 20%, more preferably at least 30%, even more preferably at least 40%, yet more preferably at least 60%, still more preferably at least 80% of the different microbes are heterologous to the seed, seedling, or other plant propagule. In certain embodiments the seeds, seedling, or other plant propagules comprise seeds, seedlings, or other plant propagules of a particular plant or class of plants. Any suitable plant or class of plants may be used, such as any one of the plants or classes of plants of section I.B.10, below. In certain embodiments, the seeds, seedlings, or other plant propagules comprise those of grass or trees. In certain embodiments the seeds, seedlings, or other plant propagules comprise grass seeds. A “grass,” as that term is used herein, includes monocotyledonous plants used for lawns, turf, or animal feed, such as pasture, fodder and rangeland grasses, and also includes monocotyledonous plants found in grasslands and in wetlands, forests, and tundra. “Grass,” as that term is used herein, does not include domesticated cereal crops. “Turf,” as that term is used herein, includes grass used for planted surfaces, such as playing surfaces, for example in fields, courses, e.g., golf courses, and pitches, for sports or other recreation. A “grassland,” includes a grass-dominated biome, such as a temperate or a tropical grassland, including but not limited to savannas, pampas, steppes, andDocket No. OATH-015-PCT2 prairies. In certain embodiments the grass seeds comprise turf seeds. In certain embodiments the seeds, seedlings, or other plant propagules of trees. In certain embodiments the composition comprises tree seeds. In certain embodiments the composition comprises tree seedlings, such as tree seedlings to be used for reforestation or upforestation, such as seelings of a conifer such as pine, or oak, or beech. In certain embodiments the composition comprises seeds, seedlings, or other plant propagules of a commercial crop. A “commercial crop,” as that term is used herein, includes a plant where the plant or one or more parts of the plant are directly or indirectly sold in a market or otherwise used in a commercial endeavor, e.g., grains (cereals), vegetables, and the like, described in more detail below. In certain cases, the commercial crop is for animal consumption, e.g., by domesticated animals, or by humans. As used herein, the term “domesticated animal” includes commercially reared avian and mammalian animals, including but not limited to poultry such as chickens, game birds, geese, turkeys and ducks, goats, sheep, alpacas, cattle, bison, horses, and pigs. In certain embodiments the commercial crop comprises a cereal crop. In certain embodiments the cereal crop comprises corn, sweet corn, popcorn, seed corn, silage corn, field corn, rice, wheat, barley, or sorghum. In certain embodiments the commercial crop comprises a berry. In certain embodiments the berry comprises blueberry, blackberry, raspberry, loganberry, huckleberry, cranberry, gooseberry, elderberry, currant, caneberry, or bushberry. In certain embodiments the commercial crop comprises an oilseed plant. In certain embodiments the oilseed plant canola, castor, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, or soybean. In certain embodiments the commercial crop comprises a tree nut. In certain embodiments the tree nut comprises almond, pistachio, pecan, walnut, filberts, chestnut, cashew, beechnut, butternut, or macadamia. In certain embodiments the commercial crop comprises a brassica vegetable. In certain embodiments the brassica vegetable comprises broccoli, cabbage, cauliflower, brussels sprouts, collards, kale, mustard greens, or kohlrabi. In certain embodiments the commercial crop comprises a cucurbit vegetable. In certain embodiments the cucurbit vegetable comprises cucumber, cantaloupe, melon, muskmelon, squash, watermelon, pumpkin, or eggplant. In certain embodiments the commercial crop comprises a bulb vegetable. In certain embodiments the bulb vegetable comprises onion, garlic, or shallots. In certain embodiments the commercial crop comprises citrus. In certain embodiments the citrus comprises orange, grapefruit, lemon, tangerine, tangelo, or pummelo. In certain embodiments the commercial crop comprises a fruiting vegetable. In certain embodiments the fruiting vegetable comprises pepper, tomato, ground cherry, tomatillo, okra, or grape. In certain embodiments the commercial crop comprises legume or vegetable such as beans. In certain embodiments the legume or vegetable such as beans comprise green beans, snap beans, shell beans, soybeans, dry beans, garbanzo beans, lima beans, peas, chickpeas, splitDocket No. OATH-015-PCT2 peas, lentils, or peanuts. In certain embodiments the commercial crop comprises a pome fruit. In certain embodiments the pome fruit comprises apple, crabapple, pear, quince, or mayhaw. In certain embodiments the commercial crop comprises a root, tuber or corm vegetable. In certain embodiments the root, tuber or corm vegetable comprises carrot, potato, sweet potato, cassava, beets, ginger, horseradish, radish, ginseng, or turnip. In certain embodiments the commercial crop comprises a stone fruit. In certain embodiments the stone fruit comprises apricot, cherry, nectarine, peach, plum, prune, or strawberry. In certain embodiments the commercial crop comprises a grass. In certain embodiments the grass comprises grass for livestock feed. In certain embodiments the grass comprises turf grass. In certain embodiments the commercial crop comprises a tree. In certain embodiments the tree comprises an evergreen tree. In certain embodiments the tree comprises a deciduous tree. In certain embodiments the commercial crop comprises an herb or spice. In certain embodiments the commercial crop comprises cannabis. In certain embodiments the commercial crop comprises an ornamental plant. In certain embodiments the ornamental plant comprises a flowering plant, a bush or a shrub. In certain embodiments the commercial crop comprises an energy or raw material plant. In certain embodiments the energy or raw material plant comprises corn, canola, sugar cane, sunflower, safflower, Jatropha, castor, oil palm, coconut, wild flax, Pongam, olive, or flax. In certain cases the energy or raw material plant is for use in producing a biofuel.
[0170] In certain embodiments, the commercial crop is corn (maize).
[0171] In certain embodiments, the commercial crop is soy.
[0172] In certain embodiments, the commercial crop is rice (e.g., dry grown rice).
[0173] In certain embodiments, the commercial crop is wheat.
[0174] In certain embodiments, the commercial crop is barley.
[0175] In certain embodiments, the commercial crop is sorghum.
[0176] In certain embodiments, the commercial crop is oat.
[0177] In certain embodiments, the commercial crop is rye.
[0178] In certain embodiments, the commercial crop is sugar cane. 8. Plants or plant parts and one or more microbes (optionally with soil).
[0179] In certain embodiments, provided are compositions that comprise a plant or plant part and one or more microbes of the microbial compositions described herein, including but not limited to, in sections I.B.1-6, for example section I.B.1, or I.B.5, or I.B.6. The plant part may be, e.g., roots of the plant. The microbes can be disposed on or in the roots, e.g., as rhizobacteria.
[0180] In certain embodiments provided is a plant or plant part comprising one or more of the microbes of the composition of any one the microbial compositions disclosed herein,Docket No. OATH-015-PCT2 including but not limited to of sections I.B.1-6, for example section I.B.1, or I.B.5, or I.B.6, disposed on or in the plant or plant part. In certain embodiments the one or more microbes are disposed on or in a plant part comprising a root of the plant. In certain embodiments at least 1, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, or 99%, or 100%, for example at least 10%, preferably at least 20%, more preferably at least 30%, even more preferably at least 40%, yet more preferably at least 60%, still more preferably at least 80% of the different microbes are heterologous to the plant or plant part. In certain embodiments the one or more microbes are present in a total amount of at least 10, 100, 200, 500, 700, 1000, 1500, 2000, 3000, 5000, 7000, 10,000, 20,000, 50,000, 100,000, 500,000, 106, 107, 108, 109, 1010, 1011, 1012, 1013and / or not more than 100, 200, 500, 700, 1000, 1500, 2000, 3000, 5000, 7000, 10,000, 20,000, 50,000, 100,000, 500,000, 106, 107, 108, 109, 1010, 1011, 1012, 1013, 1014, 1015, or 1016CFU per gram dry weight of the root, e.g., 10-1016, preferably 100-1016, more preferably 100 to 1016, even more preferably 100,000 to 1016, yet more preferably 106to 1016, yet still more preferably 107to 1016, and even yet more preferably 1012to 1016.In certain embodiments the composition further comprises mycorrhizal fungi. The plant may be any suitable plant, such as a plant described in Section B.10. In certain embodiments the plant is a grass. In certain embodiments the grass is a grassland grass. In certain embodiments the grass is a turf grass. In certain embodiments the plant is a tree. In certain embodiments the tree is a deciduous tree. In certain embodiments the tree is an evergreen tree. In certain embodiments the tree is a forest tree. In certain embodiments the plant is a commercial crop. In certain embodiments the commercial crop comprises a cereal crop. In certain embodiments the cereal crop comprises corn, sweet corn, popcorn, seed corn, silage corn, field corn, rice, wheat, barley, or sorghum. In certain embodiments the commercial crop comprises a berry. In certain embodiments the berry comprises blueberry, blackberry, raspberry, loganberry, huckleberry, cranberry, gooseberry, elderberry, currant, caneberry, or bushberry. In certain embodiments the commercial crop comprises an oilseed plant. In certain embodiments the oilseed plant canola, castor, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, or soybean. In certain embodiments the commercial crop comprises a tree nut. In certain embodiments the tree nut comprises almond, pistachio, pecan, walnut, filberts, chestnut, cashew, beechnut, butternut, or macadamia. In certain embodiments the commercial crop comprises a brassica vegetable. In certain embodiments the brassica vegetable comprises broccoli, cabbage, cauliflower, brussels sprouts, collards, kale, mustard greens, or kohlrabi. In certain embodiments the commercial crop comprises a cucurbit vegetable. In certain embodiments the cucurbit vegetable comprises cucumber, cantaloupe, melon, muskmelon, squash, watermelon, pumpkin, or eggplant. In certain embodiments the commercial crop comprises a bulb vegetable. In certain embodiments the bulb vegetable comprises onion,Docket No. OATH-015-PCT2 garlic, or shallots. In certain embodiments the commercial crop comprises citrus. In certain embodiments the citrus comprises orange, grapefruit, lemon, tangerine, tangelo, or pummelo. In certain embodiments the commercial crop comprises a fruiting vegetable. In certain embodiments the fruiting vegetable comprises pepper, tomato, ground cherry, tomatillo, okra, or grape. In certain embodiments the commercial crop comprises legume or vegetable such as beans. In certain embodiments the legume or vegetable such as beans comprise green beans, snap beans, shell beans, soybeans, dry beans, garbanzo beans, lima beans, peas, chickpeas, split peas, lentils, or peanuts. In certain embodiments the commercial crop comprises a pome fruit. In certain embodiments the pome fruit comprises apple, crabapple, pear, quince, or mayhaw. In certain embodiments the commercial crop comprises a root, tuber or corm vegetable. In certain embodiments the root, tuber or corm vegetable comprises carrot, potato, sweet potato, cassava, beets, ginger, horseradish, radish, ginseng, or turnip. In certain embodiments the commercial crop comprises a stone fruit. In certain embodiments the stone fruit comprises apricot, cherry, nectarine, peach, plum, prune, or strawberry. In certain embodiments the commercial crop comprises a grass. In certain embodiments the grass comprises grass for livestock feed. In certain embodiments the grass comprises turf grass. In certain embodiments the commercial crop comprises a tree. In certain embodiments the commercial crop comprises an evergreen tree. In certain embodiments the commercial crop comprises a deciduous tree. In certain embodiments the commercial crop comprises an herb or spice. In certain embodiments the commercial crop comprises cannabis. In certain embodiments the commercial crop comprises an ornamental plant. In certain embodiments the ornamental plant comprises a flowering plant, a bush or a shrub. In certain embodiments the commercial crop comprises an energy or raw material plant. In certain embodiments the energy or raw material plant comprises corn, canola, sugar cane, sunflower, safflower, Jatropha, castor, oil palm, coconut, wild flax, Pongam, olive, or flax. In certain cases the energy or raw material plant is for use in producing a biofuel. In certain embodiments, the plant is a corn plant. In certain embodiments, the plant is a rice plant. In certain embodiments, the plant is a soy plant. In certain embodiments, the plant is a wheat plant. In certain embodiments, the plant is a barley plant. In certain embodiments, the plant is an oat plant. In certain embodiments, the plant is a rye plant. In certain embodiments, the plant is a sugar cane plant. In certain embodiments, the plant is a sorghum plant. In any of the above embodiments, the composition may comprise roots of the plant and further comprise soil in which the plant is growing or has grown. In certain embodiments the soil is within 0.1 cm of roots of the plant. In certain embodiments the soil is within 0.5 cm of roots of the plant. In certain embodiments the soil is within 1 cm of roots of the plant. In certain embodiments the soil is within 2 cm of roots of the plant. In certain embodiments the soil is within 3 cm of roots of the plant. In certainDocket No. OATH-015-PCT2 embodiments the soil is within 5 cm of roots of the plant. In certain embodiments the soil is within 7 cm of roots of the plant. In certain embodiments the soil is within 10 cm of roots of the plant. In certain embodiments the soil is within 15 cm of roots of the plant. In certain embodiments the soil is within 20 cm of roots of the plant. In certain embodiments the soil is within 50 cm of roots of the plant. In certain embodiments the soil is within 100 cm of roots of the plant. In certain embodiments the microbes of the composition comprise at least 0.01, 0.05, 0.1, 0.5, 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, or 90% of a total number of microbes in the composition comprising soil, for example, at least 0.01%, at least 0.05% at least 01%, at least 1%, preferably at least 2%, even more preferably at least 5%, yet more preferably at least 10%, still more preferably at least 20%, even still more preferably at least 40%. In certain embodiments the composition further comprises mycorrhizal fungi. In certain embodiments the mycorrhizal fungi make up at least 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, or 90% of a total number of microbes in the composition comprising soil or example, at least 1%, preferably at least 2%, even more preferably at least 5%, yet more preferably at least 10%, still more preferably at least 20%, even still more preferably at least 40%. In certain embodiments beneficial microbes other than the microbes of the composition comprising microbes, are present at a concentration of at least 1M, 2M, 5M, 10M, 15M, 20M, 25M, 30M, 35M, 40M, 50M, 70M, or 100M and / or not more than 2M, 5M, 10M, 15M, 20M, 25M, 30M, 35M, 40M, 50M, 70M, 80M, 100M, or 1B per gram soil dry weight, for example 1M to 1B, preferably 10M to 1B, more preferably 20M to 1B, even more preferably 20M to 100M, yet more preferably 20M to 80M, still more preferably 30M to 80M per gram soil dry weight. In certain embodiments the soil has an average density of at least 0.8, 0.9, 1.0, 1.05, 1.10, 1.15, 1.20, 1.25, 1.30, 1.35, 1.40, 1.45, 1.50, 1.55, 1.60, 1.65, or 1.70 and / or not more than 0.9, 1.0, 1.05, 1.10, 1.15, 1.20, 1.25, 1.30, 1.35, 1.40, 1.45, 1.50, 1.55, 1.60, 1.65, 1.70, 1.75, 1.80, 1.85, 1.90, or 1.95, for example, 1.0- 1.90, preferably 1.05 to 1.85, more preferably 1.10 to 1.50, even more preferably 1.20-1.75, yet more preferably 1.35-1.70, still more preferably 1.55-1.85 g / cm3 dry weight of soil. In certain embodiments the soil has an average water holding capacity (WHC) of at least 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70% wt / wt and / or not more than 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70% wt / wt, for example, 20-70%, preferably 25-65%, more preferably 25-55%, yet more preferably 30-65%, still more preferably 35-65%, yet still more preferably 35-55%. As used herein, “water holding capacity” or “WHC” of soil includes weight percent of a soil or soil sample that is water when the soil or soil sample is saturated with water. Water holding capacity can be determined by any suitable method, e.g., exposing soil or a soil sample to sufficient water to saturate the soil, weighing the soil, then drying the sample. Initial weight – dry weight / initial weight x 100 = % WHC. Other methods may be used in certain instances, such as a neutron probe or moistureDocket No. OATH-015-PCT2 meter. In certain embodiments the soil has an average carbon content of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20% and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, or 40% (w / w), for example, 5-30%, preferably 7- 30%, more preferably 10-30%, yet more preferably 10-25%. in certain embodiments the soil has a soil carbon content of at least 0.5, 2, 3, 5, 7, 10, 12, 15, 20, 25, 30, or 35 kg / m3of soil and / or not more than 0.5, 2, 3, 5, 7, 10, 12, 15, 20, 25, 30, 35 or 40 kg / m3, for example 0.5-40, preferably 2-40, more preferably 5-40, even more preferably 7-35, yet more preferably 7-30, still more preferably 7-25 kg / m3of soil. In certain embodiments the soil does not comprise a significant amount or concentration of synthetic fertilizer, or a detectable amount. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well- known. In certain embodiments the soil does not comprise a detectable amount or concentration of synthetic fertilizer. In certain embodiments the soil does not comprise a significant amount or concentration of herbicide, e.g., synthetic herbicide. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well-known. In certain embodiments the soil does not comprise a detectable amount or concentration of herbicide, e.g., synthetic herbicide. In certain embodiments the soil does not comprise a significant amount or concentration of pesticide, e.g., synthetic pesticide. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well-known. In certain embodiments the soil does not comprise a detectable amount or concentration of pesticide, e.g., synthetic pesticide. In certain embodiments the soil does not comprise a significant amount or concentration of insecticide, e.g., synthetic insecticide. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well-known. In certain embodiments the soil does not comprise a detectable amount or concentration of insecticide, e.g., synthetic insecticide. In certain embodiments the soil does not comprise a significant amount or concentration of fungicide, e.g., synthetic fungicide. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well-known. In certain embodiments the soil does not comprise a detectable amount or concentration of fungicide, e.g., synthetic fungicide. In certain embodiments the soil does not comprise a significant amount or concentration of bactericide, e.g., synthetic bactericide. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well-known. In certain embodiments the soil does not comprise a detectable amount or concentration of bactericide, e.g., synthetic bactericide. In certain embodiments the soil does not comprise a significant amount or concentration of nematicide, e.g., synthetic nematicide. The amount can be, e.g., less than that allowed by organic farming criteria for the area; such criteria are well-known. In certainDocket No. OATH-015-PCT2 embodiments the soil does not comprise a detectable amount or concentration of nematicide, e.g., synthetic nematicide.
[0181] In certain embodiments provided herein is a composition comprising a plant, e.g. wherein the plant or part of the plant is for animal consumption, and soil in which the plant is growing, wherein (i) the soil and / or roots of the plant in the soil comprise one or more microbes heterologous to the soil and / or plant; and (ii) the plant or plant part comprises a density of one or more nutrients, e.g., one or more nutrients for the animal, that is greater than reference plant or plant part. In certain embodiments the composition comprises at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, or 135 different microbes and / or not more than 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135 or 140 different microbes, for example at least 20, in certain cases at least 50, in yet other cases at least 70, or preferably 10-140, more preferably 20- 120, even more preferably 40-100, yet more preferably 50-100 that are heterologous to the plant and / or soil. In certain embodiments at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, or 99%, or 100%, for example at least 10%, preferably at least 20%, more preferably at least 30%, even more preferably at least 40%, yet more preferably at least 60%, still more preferably at least 80%, yet more preferably at least 95%, yet still more preferably 100% of the different microbes are rhizobacteria. In certain embodiments at least 10%, preferably at least 20%, more preferably at least 30%, even more preferably at least 40%, yet more preferably at least 60%, still more preferably at least 80%, yet more preferably at least 95%, yet still more preferably 100% of the different microbes are saprophytic. In certain embodiments the different microbes are Streptomyces. In certain embodiments the composition comprises one or more different microbes of a microbial composition disclosed herein, including but not limited to in e.g., in sections I.B.1- 6, for example section I.B.1, or I.B.5, or I.B.6. In certain embodiments the composition comprises at least 2, 5, 7, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, or 90 and / or not more than 5, 7, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90 or 95 one or more different microbes for example at least 2, preferably at least 5, more preferably at least 10, even more preferably at least 20, yet more preferably at least 30, still more preferably at least 50, or for example 2-95, preferably 10-95, more preferably 20-95, even more preferably 30-95, yet more preferably 40-95, still more preferably 50-95. In certain embodiments nutrient density of the one or more animal nutrients is increased by at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, 150, 200, 250, 300, 350, or 400% and / or not more than 0, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, 150, 200, 250, 300, 350, 400, 500, 700, or 1000%, for example, at least 5, preferably at least 10, more preferably at least 15, even more preferably at least 20, still more preferably at least 30, yet more preferably at least 50%, or for example at least 5-1000,Docket No. OATH-015-PCT2 preferably 5-700, more preferably 10-1000, even more preferably 10-700, still more preferably 10-500, yet more preferably 20-500%. In certain embodiments the one or more nutrients is increased in density by at least 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, 150, 200, 250, 300, 350, or 400% and / or not more than 0, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, 150, 200, 250, 300, 350, 400, 500, 700, or 1000%, for example, at least 5, preferably at least 10, more preferably at least 15, even more preferably at least 20, still more preferably at least 30, yet more preferably at least 50%, or for example at least 5-1000, preferably 5-700, more preferably 10-1000, even more preferably 10-700, still more preferably 10-500, yet more preferably 20-500%. In certain embodiments the animals comprise humans or domesticated animals. In certain embodiments the animals comprise humans. In certain embodiments the animals comprise domesticated animals. In certain embodiments the animals comprise cattle, bison, goats, sheep, dairy cows, fowl (such as chickens, geese, and ducks), pigs, or horses. In certain embodiments the animals are cattle. In certain embodiments the increased nutrient density comprises increased protein density, increased mineral density, increased vitamin density, or a combination thereof. In certain embodiments the increased nutrient density comprises increased protein density. In certain embodiments the increased nutrient density comprises increased vitamin density. In certain embodiments the increased vitamin density comprises increased vitamin A density. In certain embodiments the increased vitamin density comprises increased vitamin B1(thiamine) density. In certain embodiments the increased vitamin density comprises increased vitamin B2(riboflavin) density. In certain embodiments the increased vitamin density comprises increased vitamin B3(niacin, nicotinic acid) density. In certain embodiments the increased vitamin density comprises increased vitamin B5(pantothenic acid) density. In certain embodiments the increased vitamin density comprises increased vitamin B6(pyridoxal, pyridoxine, or pyridoxamine) density. In certain embodiments the increased vitamin density comprises increased vitamin B7(biotin) density. In certain embodiments the increased vitamin density comprises increased vitamin B9(folic acid) density. In certain embodiments the increased vitamin density comprises increased vitamin B12density. In certain embodiments the increased vitamin density comprises increased vitamin C density. In certain embodiments the increased vitamin density comprises increased choline density. In certain embodiments the increased vitamin density comprises increased vitamin D density. In certain embodiments the increased vitamin density comprises increased vitamin E density. In certain embodiments the increased vitamin density comprises increased vitamin K1 density. In certain embodiments the increased vitamin density comprises increased vitamin K2 density. In certain embodiments the increased nutrient density comprises increased mineral density. In certain embodiments the increased mineral density comprises increased calcium density. In certain embodiments the increasedDocket No. OATH-015-PCT2 mineral density comprises increased magnesium density. In certain embodiments the increased mineral density comprises increased sodium density. In certain embodiments the increased mineral density comprises increased potassium density. In certain embodiments the increased mineral density comprises increased iron density. In certain embodiments the increased mineral density comprises increased copper density. In certain embodiments the increased mineral density comprises increased manganese density. In certain embodiments the increased mineral density comprises increased zinc density. In certain embodiments the increased mineral density comprises increased iodine density. In certain embodiments the increased mineral density comprises increased chromium density. In certain embodiments the increased mineral density comprises increased molybdenum density. In certain embodiments the increased mineral density comprises increased phosphorus density. In certain embodiments the increased mineral density comprises increased molybdenum density. In certain embodiments the increased mineral density comprises increased selenium density. The plant may be any suitable plant, such as a plant as disclosed herein, e.g., Section 1.B.10. 9. Populations of plants and microbes (optionally with soil)
[0182] In certain embodiments, provided are compositions comprising populations of plants comprising microbes of a microbial composition as described herein, including but not limited to those of sections I.B.1-6, for example section I.B.1, or I.B.5, or I.B.6, in certain cases also including soil the plants grow or have grown in. Thus, in certain embodiments provided is a population of plants wherein the plants or part of the plants comprises the microbe or microbes of the composition of sections I.B.1-6, for example section I.B.1, or I.B.5, or I.B.6, disposed on or in the plant or plant part. In certain embodiments the population at least 100, 1000, 5000, 10,000, 100,000, 500,000, 1M, 2M, 3M, 5M, 10M, 50M, 100M, 500M, 1B, 2B, 5B, 10B, 50B, 100B, 1T, 10T, 100T plants and / or not more than 1000, 5000, 10,000, 100,000, 500,000, 1M, 2M, 3M, 5M, 10M, 50M, 100M, 500M, 1B, 2B, 5B, 10B, 50B, 100B, 1T, 10T, 100T, or 500T plants, for example 100 to 500T, preferably 1000 to 500T, more preferably 100,000 to 500T, even more preferably 1M to 500T, yet more preferably 1B to 500T, still more preferably 10B to 500T; in some cases more than 1M, for example more than 10M, preferably more than 100M, more preferably more than 1B, even more preferably more than 10B, yet more preferably more than 100B, still more preferably more than 1T or even more than 10T, or more than 100T, or even more than 500T. Larger populations can be found in, e.g., large agricultural lands, or pasture lands, grasslands, forests, and the like. In certain embodiments, described elsewhere herein, compositions as described herein can be deposited in very large areas, such as areas of grasslands, forests, or the like, e.g., wild or undomesticated grasslands, forests, or the like; suchDocket No. OATH-015-PCT2 areas may exceed hundreds, thousands, or tens of thousands of square miles; it will be appreciated that, especially in the case of grasslands, such areas can contain billions or even trillions of individual plants. In certain embodiments at least 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 95, or 99%, or 100%, for example at least 10%, preferably at least 20%, more preferably at least 30%, even more preferably at least 40%, yet more preferably at least 60%, still more preferably at least 80% of the different microbes of the composition are heterologous to the plant or plant part. In certain embodiments the one or more microbes are disposed on or in a plant part comprising a root of the plant. In certain embodiments the one or more microbes are present in a total amount of at least 10, 100, 200, 500, 700, 1000, 1500, 2000, 3000, 5000, 7000, 10,000, 20,000, 50,000, 100,000, 500,000, 106, 107, 108, 109, 1010, 1011, 1012, 1013and / or not more than 100, 200, 500, 700, 1000, 1500, 2000, 3000, 5000, 7000, 10,000, 20,000, 50,000, 100,000, 500,000, 106, 107, 108, 109, 1010, 1011, 1012, 1013, 1014, 1015, or 1016CFU per gram dry weight of the root, e.g., 10-1016, preferably 100- 1016, more preferably 100 to 1016, even more preferably 100,000 to 1016, yet more preferably 106to 1016, yet still more preferably 107to 1016, and even yet more preferably 1012to 1016.In certain embodiments the composition further comprises mycorrhizal fungi. In certain embodiments the plants are grass. In certain embodiments the grass is a grassland grass. In certain embodiments the grass is a turf grass. In certain embodiments the plants are trees. In certain embodiments the trees are deciduous trees. In certain embodiments the trees are evergreen trees. In certain embodiments the trees are forest trees. In certain embodiments the plants are a commercial crop. In certain embodiments the commercial crop comprises a cereal crop. In certain embodiments the cereal crop comprises corn, sweet corn, popcorn, seed corn, silage corn, field corn, rice, wheat, barley, or sorghum. In certain embodiments the commercial crop comprises a berry. In certain embodiments the berry comprises blueberry, blackberry, raspberry, loganberry, huckleberry, cranberry, gooseberry, elderberry, currant, caneberry, or bushberry. In certain embodiments the commercial crop comprises an oilseed plant. In certain embodiments the oilseed plant canola, castor, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, or soybean. In certain embodiments the commercial crop comprises a tree nut. In certain embodiments the tree nut comprises almond, pistachio, pecan, walnut, filberts, chestnut, cashew, beechnut, butternut, or macadamia. In certain embodiments the commercial crop comprises a brassica vegetable. In certain embodiments the brassica vegetable comprises broccoli, cabbage, cauliflower, brussels sprouts, collards, kale, mustard greens, or kohlrabi. In certain embodiments the commercial crop comprises a cucurbit vegetable. In certain embodiments the cucurbit vegetable comprises cucumber, cantaloupe, melon, muskmelon, squash, watermelon, pumpkin, or eggplant. In certain embodiments the commercial crop comprises a bulb vegetable. In certain embodiments the bulbDocket No. OATH-015-PCT2 vegetable comprises onion, garlic, or shallots. In certain embodiments the commercial crop comprises citrus. In certain embodiments the citrus comprises orange, grapefruit, lemon, tangerine, tangelo, or pummelo. In certain embodiments the commercial crop comprises a fruiting vegetable. In certain embodiments the fruiting vegetable comprises pepper, tomato, ground cherry, tomatillo, okra, or grape. In certain embodiments the commercial crop comprises legume or vegetable such as beans. In certain embodiments the legume or vegetable such as beans comprise green beans, snap beans, shell beans, soybeans, dry beans, garbanzo beans, lima beans, peas, chickpeas, split peas, lentils, or peanuts. In certain embodiments the commercial crop comprises a pome fruit. In certain embodiments the pome fruit comprises apple, crabapple, pear, quince, or mayhaw. In certain embodiments the commercial crop comprises a root, tuber or corm vegetable. In certain embodiments the root, tuber or corm vegetable comprises carrot, potato, sweet potato, cassava, beets, ginger, horseradish, radish, ginseng, or turnip. In certain embodiments the commercial crop comprises a stone fruit. In certain embodiments the stone fruit comprises apricot, cherry, nectarine, peach, plum, prune, or strawberry. In certain embodiments the commercial crop comprises a grass. In certain embodiments the grass comprises grass for livestock feed. In certain embodiments the grass comprises turf grass. In certain embodiments the commercial crop comprises a tree. In certain embodiments the commercial crop comprises an evergreen tree. In certain embodiments the commercial crop comprises a deciduous tree. In certain embodiments the commercial crop comprises an herb or spice. In certain embodiments the commercial crop comprises cannabis. In certain embodiments the commercial crop comprises an ornamental plant. In certain embodiments the ornamental plant comprises a flowering plant, a bush or a shrub. In certain embodiments the commercial crop comprises an energy or raw material plant. In certain embodiments the energy or raw material plant comprises corn, canola, sugar cane, sunflower, safflower, Jatropha, castor, oil palm, coconut, wild flax, Pongam, olive, or flax. In certain cases the energy or raw material plant is for use in producing a biofuel. In certain embodiments, the commercial crop is a corn plant. In certain embodiments, the commercial crop is a rice plant. In certain embodiments, the commercial crop is a soy plant. In certain embodiments, the commercial crop is a wheat plant. In certain embodiments, the commercial crop is a barley plant. In certain embodiments, the commercial crop is an oat plant. In certain embodiments, the commercial crop is a rye plant. In certain embodiments, the commercial crop is a sugar cane plant. In certain embodiments, the commercial crop is a sorghum plant. In certain embodiments, the population of plants is distributed over an area of at least 0.1, 0.5, 1, 5, 10, 50, 100, 500, 1000, 5000, 10,000, 50,000, or 100,000 square miles and / or not more than 0.5, 1, 5, 10, 50, 100, 500, 1000, 5000, 10,00050,000, 100,000, 500,000, 1M, 2M, 10M, or 50M square miles, for example, 0.1 to 50M square miles, preferably 0.1 to 10M square miles, more preferably 1 toDocket No. OATH-015-PCT2 10M square miles, yet more preferably 10 to 10M square miles, still more preferably 100 to 10M square miles, yet still more preferably 1000 to 1M square miles. As described above, this can be, e.g., grassland, forest, or the like that have had compositions of microbes as described herein applied. In certain embodiments, a composition further includes soil in which the plants grow or have grown. Unless indicated otherwise, the soil can be considered to be the soil extending to the depth, or average depth, of roots of the plants. In certain embodiments the soil is within 0.1 cm of roots of the plant. In certain embodiments the soil is within 0.5 cm of roots of the plant. In certain embodiments the soil is within 1 cm of roots of the plant. In certain embodiments the soil is within 2 cm of roots of the plant. In certain embodiments the soil is within 3 cm of roots of the plant. In certain embodiments the soil is within 5 cm of roots of the plant. In certain embodiments the soil is within 7 cm of roots of the plant. In certain embodiments the soil is within 10 cm of roots of the plant. In certain embodiments the soil is within 15 cm of roots of the plant. In certain embodiments the soil is within 20 cm of roots of the plant. In certain embodiments the soil is within 50 cm of roots of the plant. In certain embodiments the soil is within 100 cm of roots of the plant. Thus, provided herein are compositions comprising a population of plants and microbes, as described herein, and soil in which the plants grow or have grown, the microbes comprise at least 0.01, 0.05, 0.1, 0.51, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, or 90% of a total number of microbes in the composition comprising soil, for example, at least 0.01%, or at least 0.1%, or 1%, preferably at least 2%, even more preferably at least 5%, yet more preferably at least 10%, still more preferably at least 20%, even still more preferably at least 40%. In certain embodiments the composition further comprises mycorrhizal fungi. In certain embodiments the mycorrhizal fungi make up at least 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80, or 90% of a total number of microbes in the composition comprising soil or example, at least 1%, preferably at least 2%, even more preferably at least 5%, yet more preferably at least 10%, still more preferably at least 20%, even still more preferably at least 40%. In certain embodiments the composition comprises beneficial microbes other than the microbes of the composition comprising microbes; these can be present, e.g., at a concentration of at least 1M, 2M, 5M, 10M, 15M, 20M, 25M, 30M, 35M, 40M, 50M, 70M, or 100M and / or not more than 2M, 5M, 10M, 15M, 20M, 25M, 30M, 35M, 40M, 50M, 70M, 80M, 100M, or 1B per gram soil dry weight, for example 1M to 1B, preferably 10M to 1B, more preferably 20M to 1B, even more preferably 20M to 100M, yet more preferably 20M to 80M, still more preferably 30M to 80M per gram soil dry weight. In certain embodiments the soil has an average density of at least 0.8, 0.9, 1.0, 1.05, 1.10, 1.15, 1.20, 1.25, 1.30, 1.35, 1.40, 1.45, 1.50, 1.55, 1.60, 1.65, or 1.70 and / or not more than 0.9, 1.0, 1.05, 1.10, 1.15, 1.20, 1.25, 1.30, 1.35, 1.40, 1.45, 1.50, 1.55, 1.60, 1.65, 1.70, 1.75, 1.80, 1.85, 1.90, or 1.95, for example, 1.0-1.90, preferably 1.05 to 1.85, more preferably 1.10 toDocket No. OATH-015-PCT2 1.50, even more preferably 1.20-1.75, yet more preferably 1.35-1.70, still more preferably 1.55- 1.85 g / cm3 dry weight of soil. In certain embodiments the soil has an average water holding capacity (WHC) of at least 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70% wt / wt and / or not more than 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70% wt / wt, for example, 20-70%, preferably 25-65%, more preferably 25-55%, yet more preferably 30-65%, still more preferably 35-65%, yet still more preferably 35-55%. In certain embodiments the soil and plant parts in the soil have an average carbon content of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20% and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, or 40% (w / w), for example, 5-30%, preferably 7-30%, more preferably 10-30%, yet more preferably 10-25%. In certain embodiments the soil has a soil carbon content of at least 0.5, 2, 3, 5, 7, 10, 12, 15, 20, 25, 30, or 35 kg / m3of soil and / or not more than 0.5, 2, 3, 5, 7, 10, 12, 15, 20, 25, 30, 35 or 40 kg / m3, for example 0.5-40, preferably 2-40, more preferably 5-40, even more preferably 7-35, yet more preferably 7-30, still more preferably 7-25 kg / m3of soil. In certain embodiments the soil is to a depth of not more than 20, 30, 40, 50, 60, 70, 80, 90, or 100 cm, for example not more than 100 cm, preferably not more than 80 cm, more preferably not more than 60 cm, even more preferably not more than 40 cm, yet more preferably not more than 30 cm, still more preferably not more than 20 cm. In certain embodiments the soil and plant parts in the soil have an average carbon content of 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 35, 37, 40, 42, 45, 50, 55, or 60 and / or not more than 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 32, 35, 37, 40, 42, 45, 50, 55, 60, or 70 metric ton per hectare, for example 10-70, preferably 10-60, more preferably 15-60, yet more preferably 15-50, still more preferably 18-60, even still more preferably 18-55 metric ton per hectare. In certain embodiments, the soil is to a depth of at least 5, 10, 15, 20, 30, 40, 50, 60, 70, 80, 90, 100, or 150 cm and / or not more than 20, 30, 40, 50, 60, 70, 80, 90, 100, 150, or 200 cm, for example not more than 100 cm, preferably not more than 80 cm, more preferably not more than 60 cm, even more preferably not more than 40 cm, yet more preferably not more than 30 cm, still more preferably not more than 20 cm, or to the depth of roots of the plants. In certain cases, for example as indicated herein, soil is less than average depth of roots, e.g., typically though not necessarily in the case of a forest. 10. Plants
[0183] In certain embodiments herein, methods or compositions include one or more plants or plant parts, or seeds, seedlings, or other plant propagules of plants, or land in which plants grow, have grown, or will grow, or other methods and compositions as described herein that comprise plants or plant parts. In certain embodiments the plants are grass. In certainDocket No. OATH-015-PCT2 embodiments the grass is a grassland grass. Grassland grasses include but are not limited to Rhodes grass, red oat grass, lemon grass, purple needlegrass, blue grama, buffalo grass, and galleta. In certain embodiments the grass is a turf grass. Turf grasses include but are not limited to annual ryegrass, bahiagrass, bermudagrass, buffalograss, carpetgrass, centipedegrass, creeping bentgrass, fine fescue, Kentucky bluegrass, perennial ryegrass, rough bluegrass, St. Augustinegrass, tall fescue, zoysiagrass, Kikuyugrass (Pennisetum clandestinum) Annual bluegrass (Poa annua), or a combination thereof. In certain embodiments the plants are trees. In certain embodiments the trees are deciduous trees. In certain embodiments the trees are evergreen trees. In certain embodiments the trees are forest trees. In certain embodiments the plants are a commercial crop. In certain embodiments the commercial crop comprises a cereal crop. In certain embodiments the cereal crop comprises corn, sweet corn, popcorn, seed corn, silage corn, field corn, rice, wheat, barley, or sorghum. In certain embodiments the commercial crop comprises a berry. In certain embodiments the berry comprises blueberry, blackberry, raspberry, loganberry, huckleberry, cranberry, gooseberry, elderberry, currant, caneberry, or bushberry. In certain embodiments the commercial crop comprises an oilseed plant. In certain embodiments the oilseed plant canola, castor, coconut, cotton, flax, oil palm, olive, peanut, rapeseed, safflower, sesame, sunflower, or soybean. In certain embodiments the commercial crop comprises a tree nut. In certain embodiments the tree nut comprises almond, pistachio, pecan, walnut, filberts, chestnut, cashew, beechnut, butternut, or macadamia. In certain embodiments the commercial crop comprises a brassica vegetable. In certain embodiments the brassica vegetable comprises broccoli, cabbage, cauliflower, brussels sprouts, collards, kale, mustard greens, or kohlrabi. In certain embodiments the commercial crop comprises a cucurbit vegetable. In certain embodiments the cucurbit vegetable comprises cucumber, cantaloupe, melon, muskmelon, squash, watermelon, pumpkin, or eggplant. In certain embodiments the commercial crop comprises a bulb vegetable. In certain embodiments the bulb vegetable comprises onion, garlic, or shallots. In certain embodiments the commercial crop comprises citrus. In certain embodiments the citrus comprises orange, grapefruit, lemon, tangerine, tangelo, or pummelo. In certain embodiments the commercial crop comprises a fruiting vegetable. In certain embodiments the fruiting vegetable comprises pepper, tomato, ground cherry, tomatillo, okra, or grape. In certain embodiments the commercial crop comprises legume or vegetable such as beans. In certain embodiments the legume or vegetable such as beans comprise green beans, snap beans, shell beans, soybeans, dry beans, garbanzo beans, lima beans, peas, chickpeas, split peas, lentils, or peanuts. In certain embodiments the commercial crop comprises a pome fruit. In certain embodiments the pome fruit co...
Claims
Docket No. OATH-015-PCT2 CLAIMS WHAT IS CLAIMED IS:
1. A composition comprising one or more Streptomyces microbes, wherein the one or more Streptomyces microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 68-109, 120-198, 202-209, 211-220, 222-246, 248-250, 252-256, 258- 278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375- 377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.
2. The composition of claim 1 wherein the one or more Streptomyces microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of 2SEQ ID NOs: 197, 198, 202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296- 299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.
3. The composition of claim 1 or 2 wherein each microbe is present in a concentration of at least 105, 106, 107, 108, 109, or 1010CFU / gm and / or not more than 106, 107, 108, 109, 1010or 1011CFU / gm, preferably 105to 1010CFU / gm, more preferably 106to 1010CFU / gm, yet more preferably 107to 1010CFU / gm, even more preferably 106to 1010CFU / gm.
4. The composition of any previous claim comprising microbes of at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, or 60 and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, 60, 70, 80, or 100, preferably 2-100, more preferably 5-80, even more preferably 5-40, still more preferably 5-30, yet more preferably 10-80, still yet more preferably 10-70, even more preferably 10-60, yet even more preferably 10-30 different Streptomyces strains.
5. The composition of any previous claim further comprising one or more non-Streptomyces microbes.Docket No. OATH-015-PCT2 6. The composition of claim 5 comprising one or more Pseudomonas microbes.
7. The composition of claim 6 wherein the one or more Pseudomonas microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 281, 304, 305, 456- 458, 459, 466, and / or 482.
8. The composition of any one of claims 5 through 7 comprising one or more Microvirga microbes.
9. The composition of claim 8 wherein the one or more Microvirga microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
417.
10. The composition of any one of claims 5 through 9 comprising one or more Paenibacillus microbes 11. The composition of claim 10 wherein the one or more Paenibacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 199, 323, and / or 393.
12. The composition of any one of claims 5 through 11 comprising one or more Rhodococcus microbes.
13. The composition of claim 12 wherein the one or more Rhodococcus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even moreDocket No. OATH-015-PCT2 preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
399.
14. The composition of any one of claims 5 through 13 comprising one or more Lysinibacillus microbes.
15. The composition of claim 14 wherein the one or more Lysinibacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 279, and / or 395-397.
16. The composition of any one of claims 5 through 15 comprising Actinoplanes microbes.
17. The composition of claim 16 wherein the one or more Actinoplanes microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
409.
18. The composition of any one of claims 5 through 17 comprising Agromyces microbes.
19. The composition of claim 18 wherein the one or more Agromyces microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
418.
20. The composition of any one of claims 5 through 19 comprising Allosphingosinicella microbes.
21. The composition of claim 20 wherein the one or more Allosphingosinicella microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, evenDocket No. OATH-015-PCT2 more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
432.
22. The composition of any one of claims 5 through 21 comprising Aneurinibacillus microbes.
23. The composition of claim 22 wherein the one or more Aneurinibacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 294 and / or 327.
24. The composition of any one of claims 5 through 23 comprising Arthrobacter microbes.
25. The composition of claim 24 wherein the one or more Arthrobacter microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 407, 441, 452, 455, 468, 469, and / or 473-476.
26. The composition of any one of claims 5 through 25 comprising Bacillus microbes. The composition of claim 26 wherein the one or more Bacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 200, 201, 289, 295, 406, 415, 416, 419, 420, 435, 438, 444-446, 451, 460-465, 470, 478, 479, and / or 483-486.
28. The composition of any one of claims 5 through 27 comprising Brevibacillus microbes.
29. The composition of claim 28 wherein the one or more Brevibacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even moreDocket No. OATH-015-PCT2 preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
300.
30. The composition of any one of claims 5 through 29 comprising Caldimonas microbes.
31. The composition of claim 30 wherein the one or more Caldimonas microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
440.
32. The composition of any one of claims 5 through 31 comprising Cupriavidus microbes.
33. The composition of claim 32 wherein the one or more Cupriavidus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NOs:
488.
34. The composition of any one of claims 5 through 33 comprising Erythrobacter microbes.
35. The composition of claim 34 wherein the one or more Erythrobacter microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
422.
36. The composition of any one of claims 5 through 35 comprising Kocuria microbes.
37. The composition of claim 36 wherein the one or more Kocuria microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 405, 408, 436, and / or 443.Docket No. OATH-015-PCT2 38. The composition of any one of claims 5 through 37 comprising Microbacterium microbes.
39. The composition of claim 38 wherein the one or more Microbacterium microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 210, 247, 345, 356, and / or 374.
40. The composition of any one of claims 5 through 39 comprising Microbispora microbes.
41. The composition of claim 40 wherein the one or more Microbispora microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
421.
42. The composition of any one of claims 5 through 41 comprising Mycetocola microbes.
43. The composition of claim 42 wherein the one or more Mycetocola microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
433.
44. The composition of any one of claims 5 through 43 comprising Neorhizobium microbes.
45. The composition of claim 44 wherein the one or more Neorhizobium microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 404, 410, 411, and / or 413.Docket No. OATH-015-PCT2 46. The composition of any one of claims 5 through 45 comprising Nocardia microbes.
47. The composition of claim 46 wherein the one or more Nocardia microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
424.
48. The composition of any one of claims 5 through 47 comprising Nocardioides microbes.
49. The composition of claim 48 wherein the one or more Nocardioides microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
434.
50. The composition of any one of claims 5 through 49 comprising Nonomuraea microbes.
51. The composition of claim 50 wherein the one or more Nonomuraea microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
412.
52. The composition of any one of claims 5 through 51 comprising Peribacillus microbes.
53. The composition of claim 52 wherein the one or more Peribacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 467, 471, and / or 477.
54. The composition of any one of claims 5 through 53 comprising Priestia microbes.Docket No. OATH-015-PCT2 55. The composition of claim 54 wherein the one or more Priestia microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 401, 402, 447-450, 453, 454, 480, and / or 481.
56. The composition of any one of claims 5 through 55 comprising Pristimantibacillus microbes.
57. The composition of claim 56 wherein the one or more Pristimantibacillus microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
324.
58. The composition of any one of claims 5 through 57 comprising Stenotrophomonas microbes.
59. The composition of claim 58 wherein the one or more Stenotrophomonas microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of one or more of SEQ ID NOs: 310-314.
60. The composition of any one of claims 5 through 59 comprising Stutzerimonas microbes.
61. The composition of claim 60 wherein the one or more Stutzerimonas microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
439.
62. The composition of any one of claims 5 through 61 comprising Terrimonas microbes.Docket No. OATH-015-PCT2 63. The composition of claim 62 wherein the one or more Terrimonas microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
442.
64. The composition of any one of claims 5 through 63 comprising Zhihengliuella microbes.
65. The composition of claim 64 wherein the one or more Zhihengliuella microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identity to a nucleic acid sequence of SEQ ID NO:
472.
66. The composition of any one of the previous claims wherein two or more of the microbes are heterologous to each other.
67. A composition comprising at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, or 60 and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, 60, 70, 80, or 100, preferably 2-100, more preferably 5- 80, even more preferably 5-40, still more preferably 5-30, yet more preferably 10-80, still yet more preferably 10-70, even more preferably 10-60, yet even more preferably 10-30 different strains of Streptomyces microbes.
68. The composition of claim 67 wherein the strains of the Streptomyces microbes comprise at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, or 60 and / or not more than 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 25, 30, 35, 40, 50, 60, 70, 80, or 100, preferably 2-100, more preferably 5-80, even more preferably 5-40, still more preferably 5-30, yet more preferably 10-80, still yet more preferably 10-70, even more preferably 10-60, yet even more preferably 10-30 different strains shown in Table 2A.
69. The composition of claim 67 or 68 further comprising one or more non-Streptomyces microbes.Docket No. OATH-015-PCT2 70. The composition of any one of claims 67 through 69 wherein the one or more non- Streptomyces microbes comprise a Pseudomonas, Microvirga, Paenibacillus, Rhodococcus, Lysinibacillis, Actinoplanes, Agromyces, Allosphingosinicella, Aneurinibacillus, Arthrobacter, Bacillus, Brevibacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbacterium, Microbispora, Mycetocola, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pristimantibacillus, Stenotrophomonas, Stutzerimonas, Terrimonas, or Zhihengliuella microbe, or a combination thereof.
71. The composition of claim 70 wherein the one or more non-Streptomyces microbes comprise one or more Pseudomonas microbes.
72. The composition of claim 70 or 71 comprising a Microvirga microbe.
73. The composition of any one of claims 70 through 72 wherein the one or more non- Streptomyces microbes comprise one or more Paenibacillus microbes.
74. The composition of any one of claims 70 through 73 wherein the one or more non- Streptomyces microbes comprise one or more Rhodococcus microbes.
75. The composition of any one of claims 70 through 74 wherein the one or more non- Streptomyces microbes comprise one or more Lysinibacillus microbes.
76. The composition of any one of claims 70 through 75 wherein the one or more non- Streptomyces microbes comprise one or more. Actinoplanes microbes.
77. The composition of any one of claims 70 through 76 through wherein the one or more non-Streptomyces microbes comprise one or more Agromyces microbes.
78. The composition of any one of claims 70 through 77 wherein the one or more non- Streptomyces microbes comprise one or more Allosphingosinicella microbes.
79. The composition of any one of claims 70 through 78 wherein the one or more non- Streptomyces microbes comprise one or more Aneurinibacillus microbes.Docket No. OATH-015-PCT2 80. The composition of any one of claims 70 through 79 wherein the one or more non- Streptomyces microbes comprise one or more Arthrobacter microbes.
81. The composition of any one of claims 70 through 80 wherein the one or more non- Streptomyces microbes comprise one or more Bacillus microbes.
82. The composition of any one of claims 70 through 81 wherein the one or more non- Streptomyces microbes comprise one or more Brevibacillus microbes.
83. The composition of any one of claims 70 through 82 wherein the one or more non- Streptomyces microbes comprise one or more Caldimonas microbes.
84. The composition of any one of claims 70 through 83 wherein the one or more non- Streptomyces microbes comprise one or more Cupriavidus microbes.
85. The composition of any one of claims 70 through 84 wherein the one or more non- Streptomyces microbes comprise one or more Erythrobacter microbes.
86. The composition of any one of claims 70 through 85 wherein the one or more non- Streptomyces microbes comprise one or more Kocuria microbes.
87. The composition of any one of claims 70 through 86 wherein the one or more non- Streptomyces microbes comprise one or more Microbacterium microbes.
88. The composition of any one of claims 70 through 87 wherein the one or more non- Streptomyces microbes comprise one or more Microbispora microbes.
89. The composition of any one of claims 70 through 88 wherein the one or more non- Streptomyces microbes comprise one or more Mycetocola microbes.
90. The composition of any one of claims 70 through 89 wherein the one or more non- Streptomyces microbes comprise one or more Neorhizobium microbes.
91. The composition of any one of claims 70 through 90 wherein the one or more non- Streptomyces microbes comprise one or more Nocardia microbes.Docket No. OATH-015-PCT2 92. The composition of any one of claims 70 through 91 wherein the one or more non- Streptomyces microbes comprise one or more Nocardioides microbes.
93. The composition of any one of claims 70 through 92 wherein the one or more non- Streptomyces microbes comprise one or more Nonomuraea microbes.
94. The composition of any one of claims 70 through 93 wherein the one or more non- Streptomyces microbes comprise one or more Peribacillus microbes.
95. The composition of any one of claims 70 through 94 wherein the one or more non- Streptomyces microbes comprise one or more Priestia microbes.
96. The composition of any one of claims 70 through 95 wherein the one or more non- Streptomyces microbes comprise one or more Pristimantibacillus microbes. The composition of any one of claims 70 through 96 wherein the one or more non- Streptomyces microbes comprise one or more Stenotrophomonas microbes.
98. The composition of any one of claims 70 through 97 wherein the one or more non- Streptomyces microbes comprise one or more Stutzerimonas microbes.
99. The composition of any one of claims 70 through 98 wherein the one or more non- Streptomyces microbes comprise one or more Terrimonas microbes.
100. The composition of any one of claims 70 through 99 wherein the one or more non- Streptomyces microbes comprise one or more Zhihengliuella microbes.
101. The composition of any one of claims 67 through 100 comprising one or more of the microbes of Table 2A.
102. The composition of any one of claims 67 through 100 wherein the one or more microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 68-Docket No. OATH-015-PCT2 109, 120-198, 202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.
103. The composition of any one of claims 67 through 100 wherein the one or more microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 197, 198202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296- 299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.
104. A composition comprising Streptomyces spp. microbes and cells of at least one, two, three, four, five, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 20, 22, 25, 27, 30, or 32 and / or not more than one, two, three, four, five, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 20, 22, 25, 27, 30, 32, or 35 preferably 2-35, more preferably 3-35, yet more preferably 5-35, , even yet more preferably 5-20, still more preferably 5-15, such as 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, or 35 additional microbial species selected from the group consisting of Pseudomonas spp., Microvirga spp., Paenibacillus spp., Rhodococcus spp., Lysinibacillis spp., Actinoplanes spp., Agromyces spp., Allosphingosinicella spp., Aneurinibacillus spp., Arthrobacter spp., Bacillus spp., , Brevibacillus spp., Caldimonas spp., Cupriavidus spp., Erythrobacter spp., Kocuria spp., Microbacterium spp., Microbispora spp., Mycetocola spp., Neorhizobium spp., Nocardia spp., Nocardioides spp., Nonomuraea spp., Peribacillus spp., Priestia spp., Pristimantibacillus spp., Stenotrophomonas spp., Stutzerimonas spp., Terrimonas spp., and Zhihengliuella spp.
105. The composition of claim 104 comprising microbes of at least 2, 3, 5, 7, 10, 12, 15, 20, 30, 40, or 50 and / or not more than 3, 5, 7, 10, 12, 15, 20, 30, 40, 50, 60, 80, or 100 different Streptomyces strains, for example 2-100 different Streptomyces strains.
106. The composition of claim 104 or claim 105 comprising Pseudomonas spp. microbes.Docket No. OATH-015-PCT2 107. The composition of claim 106 comprising microbes of at least 2, 3, 4, 5, 6, 7, 8, 10, 12, 14, 16 or 18 and / or not more than 3, 4, 5, 6, 7, 8, 10, 12, 14, 16, 18, 20 or 24 different Pseudomonas species, for example 2-24 different Pseudomonas strains.
108. The composition any one of claims 104 through 107 comprising Microvirga spp. microbes.
109. The composition of any one of claims 104 through 108 comprising Paenibacillus spp. microbes.
110. The composition of claim 109 comprising microbes of at least 2, 3, 4, 5, 6, or 7 and / or not more than 3, 4, 5, 6, 7, 8, or 10 different Paenibacillus species, for example 2-10 different Paenibacillus strains.
111. The composition of any one of claims 104 through 110 comprising microbes g Rhodococcus spp. microbes.
112. The composition of claim 111 comprising microbes of at least 1, or at least 2 different Rhodococcus strains.
113. The composition of any one of claims 104 through 112 comprising microbes Lysinibacillis spp. microbes.
114. The composition of claim 113 comprising microbes of at least 1, 2, 3, 4, 5, 6, or 7 and / or not more than 3, 4, 5, 6, 7, 8, or 10 different Lysinibacillus species, for example 1-10, preferably 1-5, more preferably 1, or 2, different Lysinibacillus strains.
115. The composition of any one of claims 104 through 114 comprising microbes Actinoplanes spp microbes.
116. The composition of any one of claims 104 through 115 comprising microbes Agromyces spp microbes.
117. The composition of any one of claims 104 through 116 comprising microbes Allosphingosinicella spp microbes.Docket No. OATH-015-PCT2 118. The composition of any one of claims 104 through 117 comprising microbes Aneurinibacillus spp microbes.
119. The composition of of any one of claims 104 through 118 comprising Arthrobacter spp microbes.
120. The composition of claim 119 comprising microbes of at least 2, 3, 4, 5, 6, or 7 and / or not more than 3, 4, 5, 6, 7, 8, or 10 different Arthrobacter species, for example 2-10 different Arthrobacter strains.
121. The composition of of any one of claims 104 through 120 comprising Bacillus spp microbes.
122. The composition of claim 121 comprising microbes of at least 1, 2, 3, 4, 5, 6, or 7 and / or not more than 2, 3, 4, 5, 6, 7, 8, or 10 different Bacillus species, for example 1-10, preferably 2- 10 different Bacillus strains.
123. The composition of of any one of claims 104 through 122 comprising Brevibacillus spp microbes.
124. The composition of of any one of claims 104 through 123 comprising Caldimonas spp microbes.
125. The composition of of any one of claims 104 through 124 comprising Cupriavidus spp microbes.
126. The composition of of any one of claims 104 through 125 comprising g Erythrobacter spp microbes.
127. The composition of of any one of claims 104 through 126 comprising Kocuria spp microbes.
128. The composition of of any one of claims 104 through 127 comprising Microbacterium spp microbes.Docket No. OATH-015-PCT2 129. The composition of claim 128 comprising microbes of at least 1, 2, 3, 4, or 5 and / or not more than 2, 3, 4, 5, or 6 different Microbacterium species, for example 1-6, preferably 1-4, different Microbacterium strains.
130. The composition of any one of claims 104 through 129 comprising Microbispora spp microbes.
131. The composition of any one of claims 104 through 130 comprising Mycetocola spp microbes.
132. The composition of any one of claims 104 through 131 comprising Neorhizobium spp microbes.
133. The composition of claim 132 comprising microbes of 1, or microbes of 2, different Neorhizobium strains.
134. The composition of any one of claims 104 through 133 comprising Nocardia spp microbes.
135. The composition of claim 134 comprising microbes of 1, or microbes of 2, different Nocardia strains.
136. The composition of any one of claims 104 through 135 comprising Nocardioides spp microbes.
137. The composition of any one of claims 104 through 136 comprising Nonomuraea spp microbes.
138. The composition of any one of claims 104 through 137 comprising Peribacillus spp microbes.
139. The composition of any one of claims 104 through 138 comprising Priestia spp microbes.Docket No. OATH-015-PCT2 140. The composition of claim 139 comprising microbes of 1, or microbes of 2, different Priestia strains.
141. The composition of any one of claims 104 through 140 comprising Pristimantibacillus spp microbes.
142. The composition of any one of claims 104 through 141 comprising Stenotrophomonas spp microbes.
143. The composition of any one of claims 104 through 142 comprising Stutzerimonas spp microbes.
144. The composition of any one of claims 104 through 143 comprising Terrimonas spp microbes.
145. The of any one of claims 104 through 144 comprising Zhihengliuella spp microbes.
146. The composition of any one of claims 104 through 145 wherein the one or more microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 68- 109, 120-198, 202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296-299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.
147. The composition of any one of claims 104 through 145 wherein the one or more microbes comprise a 16S rRNA gene nucleotide sequence that is at least 80, 85, 90, 95, 96, 97, 98, 99, or 99.5 identical, or 100% identical, such as at least 95%, preferably at least 96%, more preferably at least 97%, even more preferably at least 98%, still more preferably at least 99%, yet still more preferably at least 99.5% identical to a nucleic acid sequence of one or more of SEQ ID NOs: 197, 198202-209, 211-220, 222-246, 248-250, 252-256, 258-278, 280, 282-288, 290-293, 296- 299, 301-303, 306-322, 326, 328-344, 346-351, 357-373, 375-377, 379-392, 394, 398, 403, 414, 423, 425-431, and / or 437.Docket No. OATH-015-PCT2 148. A composition comprising microbes from the genera Microbacterium, Rhodococcus, and Streptomyces.
149. The composition of claim 148 wherein Streptomyces comprise one or more strains of the species Streptomyces griseoincarnatus, Streptomyces misionensis, Streptomyces murinus, Streptomyces echinatu, Streptomyces bacillari, Streptomyces sennicomposti, Streptomyces chartreusis, Streptomyces globosus, Streptomyces achromogenes, and / or Streptomyces eurythermus.
150. The composition of claim 148 or 149 wherein Microbacterium comprises one or more strains of the species Microbacterium paraoxydans.
151. The composition of any one of claims claim 148 through 150 wherein Rhodococcus comprises one or more strains of the species Rhodococcus erythropolis.
152. The composition of any one of claims claim 148 through 151 comprising one or more of the bacteria of Table 33.
153. A composition comprising microbes from the genera Lysinibacillus, Microbacterium, Rhodococcus, and Streptomyces.
154. The composition of claim 153 wherein the Streptomyces bacteria comprise one or more strains of the species Streptomyces griseoincarnatus, Streptomyces griseoaurantiacus, Streptomyces rochei, Streptomyces bikiniensis, Streptomyces misionensis, Streptomyces murinus, Streptomyces bacillari, Streptomyces sennicomposti, and / or Streptomyces achromogenes.
155. The composition of claim 153 or 154 wherein the Lysinibacillus bacteria comprise one or more strains of the species Lysinibacillus sphaericus.
156. The composition of any one of claims 153 through 155 wherein the Microbacterium bacteria comprise one or more strains of the species Microbacterium paraoxydans.
157. The composition of any one of claims 153 through 156 wherein the Rhodococcus bacteria comprise one or more strains of the species Rhodococcus erythropolis.Docket No. OATH-015-PCT2 158. The composition of any one of claims 153 through 157 comprising one or more of the bacteria of Table 34.
159. A composition comprising microbes from the genera Lysinibacillus, Microbacterium, Rhodococcus, and Streptomyces.
160. The composition of claim 159 wherein the Lysinibacillus bacteria comprise one or more strains of the species Lysinibacillus sphaericus.
161. The composition of claim 159 or 160 wherein the Microbacterium bacteria comprise one or more strains of the species Microbacterium paraoxydans.
162. The composition of any one of claims 159 through 161 wherein the Rhodococcus bacteria comprise one or more strains of the species Rhodococcus erythropolis.
163. The composition of any one of claims 159 through 162 wherein the Streptomyces bacteria comprise one or more strains of the species Streptomyces misionensis, Streptomyces bacillari, Streptomyces achromogenes, and / or Streptomyces globosus.
164. The composition of any one of claims 159 through 163 comprising one or more of the bacteria of Table 37.
165. A composition comprising microbes from the genera Bacillus, Lysinibacillus, Rhodococcus, and Streptomyces.
166. The composition of claim 165 wherein the Lysinibacillus bacteria comprise one or more strains of the species Lysinibacillus sphaericus.
167. The composition of claim 165 or 166 wherein the Rhodococcus bacteria comprise one or more strains of the species Rhodococcus erythropolis.
168. The composition of any one of claims 165 through 167 wherein the Bacillus bacteria comprise one or more strains of the species Bacillus velizensis.Docket No. OATH-015-PCT2 169. The composition of any one of claims 165 through 168 wherein the Streptomyces bacteria comprise one or more strains of the species Streptomyces misionensis, Streptomyces bacillari, Streptomyces achromogenes, Streptomyces globosus, Streptomyces antibioticus, Streptomyces bikiniensis, Streptomyces chartreusis Streptomyces echinatu, Streptomyces griseoaurantiacus, Streptomyces murinus, Streptomyces musisoli, Streptomyces olivaceus, Streptomyces sennicomposti, and / or Streptomyces toxytricini.
170. The composition of any one of claims 165 through 169 comprising one or more of the bacteria of Table 38.
171. A composition comprising microbes from the genera Lysinibacillus, Microbacterium, Rhodococcus, and Streptomyces.
172. The composition of claim 171 wherein the Lysinibacillus bacteria comprise one or more strains of the species Lysinibacillus sphaericus.
173. The composition of claim 171 or 172 wherein the Rhodococcus bacteria comprise one or more strains of the species Rhodococcus erythropolis.
174. The composition of any one of claims 171 through 173 wherein the Microbacterium bacteria comprise one or more strains of the species Microbacterium paraoxydans.
175. The composition of any one of claims 171 through 174 wherein the Streptomyces bacteria comprise one or more strains of the species Streptomyces achromogenes, Streptomyces bacillari, Streptomyces globosus, Streptomyces misionensis, and / or Streptomyces rochei.
176. The composition of any one of claims 171 through 175 comprising one or more of the bacteria of Table 40.
177. A composition comprising microbes from the genera Microbacterium, Rhodococcus, and Streptomyces.
178. The composition of claim 177 wherein the Rhodococcus bacteria comprise one or more strains of the species Rhodococcus erythropolis.Docket No. OATH-015-PCT2 179. The composition of claim 177 or 178 wherein the Microbacterium bacteria comprise one or more strains of the species Microbacterium paraoxydans.
180. The composition of any one of claims 177 through 179 wherein the Streptomyces bacteria comprise one or more strains of the species Streptomyces achromogenes, Streptomyces bacillari, Streptomyces bikiniensis ,Streptomyces globosus, Streptomyces griseoincarnatus, Streptomyces misionensis, Streptomyces murinus, and / or Streptomyces rochei.
181. The composition of any one of claims 177 through 180 comprising one or more of the bacteria of Table 41.
182. A composition comprising microbes from the genera Bacillus, Lysinibacillus, Microbacterium, Neorhizobium, Peribacillus, Priestia, Pseudomonas, Rhodococcus, and Streptomyces.
183. The composition of claim 182 wherein the Rhodococcus bacteria comprise one or more strains of the species Rhodococcus erythropolis.
184. The composition of claim 182 or 183 wherein the Microbacterium bacteria comprise one or more strains of the species Microbacterium paraoxydans.
185. The composition of any one of claims 182 through 184 wherein the Bacillus bacteria comprise one or more strains of species Bacillus spizizenii and / or Bacillus paralicheniformis.
186. The composition of any one of claims 182 through 185 wherein the Neorhizobium bacteria comprise one or more strains of species Neorhizobium flavum.
187. The composition of any one of claims 182 through 186 wherein the Peribacillus bacteria comprise one or more strains of species Peribacillus frigoritolerans_A.
188. The composition of any one of claims 182 through 187 wherein the Priestia bacteria comprise one or more strains of species Priestia aryabhattai.
189. The composition of any one of claims 182 through 188 wherein the Streptomyces bacteria comprise one or more strains of species Streptomyces nigrescens, Streptomiyces nigra, Streptomyces bobili, Streptomyces albogriseolus, Streptomyces globosus, StreptomycesDocket No. OATH-015-PCT2 griseoincarnatus, Streptomyces griseoaurantiacus, Streptomyces rochei, Streptomyces bikiniensis, Streptomyces misionensis, Streptomyces murinus, Streptomyces bacillari, Streptomyces sennicomposti, and / or Streptomyces achromogenes.
190. The composition of any one of claims 182 through 189 comprising one or more of the bacteria of Table 35.
191. A composition comprising one or more of the bacteria of Table 42.
192. A composition comprising one or more of the bacteria of Table 43.
193. A composition comprising bacteria of the genus Streptomyces and bacteria of the class Bacilli.
194. The composition of claim 193 wherein the bacteria of the class Bacilli comprise bacteria of one or more of Aneurinibacillus, e.g., one or more strains of the species Aneurinibacillus aneurinilyticus; Bacillus, e.g., one or more strains of the species Bacillus_I endophytica_A, Bacillus haynesii, Bacillus inaqosorum, Bacillus licheniformis, Bacillus paralicheniformis, Bacillus safensis, Bacillus spizizenii, Bacillus subtilis, Bacillus_A thuringiensis_K, and / or Bacillus velizensis; Brevibacillus, such as one or more strains of the species Brevibacillus panacihumi; Lysinibacillus, such as one or more strains of the species Lysinibacillus sphaericus and / or Lysinibacillus capsica; Paenibacillus, such as one or more strains of the species Paenibacillus cellulositrophicus; Peribacillus, such as one or more strains of the species Peribacillus frigoritolerans_A; and / or Pristimantibacillus.
195. The composition of claim 193 or 194 comprising one or more of the Streptomyces species or strains of the species as shown in Table 2A and one or more of the Bacilli genera, species or strains of the species as shown in Table 2A.
196. A composition comprising bacteria from one or more of genera Actinoplanes, Agromyces, Allosphingosinicella, Arthrobacter, Bacillus, Caldimonas, Cupriavidus, Erythrobacter, Kocuria, Microbispora, Microvirga, Mycetocola, Neorhizobium, Nocardia, Nocardioides, Nonomuraea, Peribacillus, Priestia, Pseudomonas, Streptomyces, Stutzerimonas, Terrimonas, and / or Zhihengliuella.Docket No. OATH-015-PCT2 197. The composition of claim 196 comprising one or more of the strains of Table 44.
198. The composition of claim 196 or 197 comprising free-living nitrogen-fixing bacteria.
199. The composition of any one of the previous claims comprising one or more microbes of ATCC deposits designated PTA-127834-127850 respectively, as shown in Table 2A.
200. A composition comprising one or more microbes of ATCC deposits designated Nos. PTA-127834-127850 respectively, as shown in Table 2A.
201. The composition of claim 199 or claim 200 comprising one or more bacteria of ATCC deposit number PTA-127834, and / or one or more bacteria of ATCC deposit number PTA- 127835, and / or one or more bacteria of ATCC deposit number PTA-127836, and / or one or more bacteria of ATCC deposit number PTA-127837, and / or one or more bacteria of ATCC deposit number PTA-127838, and / or one or more bacteria of ATCC deposit number PTA-127839, and / or one or more bacteria of ATCC deposit number PTA-127840, and / or one or more bacteria of ATCC deposit number PTA-127841, and / or one or more bacteria of ATCC deposit number PTA- 127842, and / or one or more bacteria of ATCC deposit number PTA-127843, and / or one or more bacteria of ATCC deposit number PTA-127844, and / or one or more bacteria of ATCC deposit number PTA-127845, and / or one or more bacteria of ATCC deposit number PTA-127846, and / or one or more bacteria of ATCC deposit number PTA-127847, and / or one or more bacteria of ATCC deposit number PTA-127848, and / or one or more bacteria of ATCC deposit number PTA- 127849, and / or one or more bacteria of ATCC deposit number PTA-127850.
202. The composition of any one of claims 199 through 201 wherein the composition comprises one or more bacteria of the genus Microbacterium from ATCC deposit number PTA- 127834 and / or the composition comprises one or more bacteria of the genus Rhodococcus from ATCC deposit number PTA-127834 and / or the composition comprises one or more bacteria of the genus Arthrobacter from ATCC deposit number PTA-127834 and / or the composition comprises one or more bacteria of the genus Aneurinibacillus from ATCC deposit number PTA- 127839 and / or the composition comprises one or more bacteria of the genus Psuedomonas from ATCC deposit number PTA-127844 and / or the composition comprises one or more bacteria of the genus Bacillus from ATCC deposit number PTA-127847 and / or the composition comprises one or more bacteria of the genus Paenibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Pristimantibacillus from ATCCDocket No. OATH-015-PCT2 deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Brevibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Paenibacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the Bacillus from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Microvirga from ATCC deposit number PTA-127848 and / or the composition comprises one or more bacteria of the genus Psuedomonas from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Peribacillus from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Zhihengliuella from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Nonomuraea from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Nocardia from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Kocuria from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Actinoplanes from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Microbispora from ATCC deposit number PTA- 127849 and / or the composition comprises one or more bacteria of the genus Mycetocola from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Nocardioides from ATCC deposit number PTA-127849 and / or the composition comprises one or more bacteria of the genus Priestia from ATCC deposit number PTA-127850 and / or the the composition comprises one or more bacteria of the genus Cupriavidus from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Peribacillus from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Stutzerimonas from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Caldimonas from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Terrimonas from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Neorhizobium from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Erythrobacter from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Allosphingosinicella from ATCC deposit number PTA-127850 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127834 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127835 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127836 and / or the compositionDocket No. OATH-015-PCT2 comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127837 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127838 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127839 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127840 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127841 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127842 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127843 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127844 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127845 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA- 127846 and / or the composition comprises one or more bacteria of the genus Streptomyces from ATCC deposit number PTA-127849.
203. The composition of any one of the previous claims comprising one or more fungal microbes.
204. A composition comprising fungal microbes.
205. The composition of claim 203 or 204 wherein the fungal microbes comprise ectomycorrhizal fungi.
206. The composition of claim 205 wherein the fungal microbes comprise one or more of Aspergillus, Chaetomium, Coniothyrium, Paecilomyces, Penicillium, Phlebia, Rhizopogon, Scleroderma, Lecanicillium, and / or Sebacina microbes.
207. The composition of claim 206 comprising one or more Aspergillus microbes.
208. The composition of claim 207 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Aspergillus microbes, for example 1-20 different Aspergillus microbes, preferably 1 to 16 different 208Aspergillus microbes, more preferably 1 to 10 different Aspergillus microbes, even more preferably 2 to 10 different Aspergillus microbes.Docket No. OATH-015-PCT2 209. The composition of claim 207 or 208 wherein the Aspergillus microbes comprise Aspergillus flavus.
210. The composition of any one of claims 207 through 209 wherein the Aspergillus microbes comprise Aspergillus niger.
211. The composition of any one of claims 205 through 208 comprising one or more Chaetomium microbes.
212. The composition of claim 211 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Chaetomium microbes, for example 1-20 different Chaetomium microbes, preferably 1 to 16 different Chaetomium microbes, more preferably 1 to 10 different Chaetomium microbes, even more preferably 2 to 10 different Chaetomium microbes.
213. The composition of any one of claims 205 through 212 comprising one or more Coniothyrium microbes.
214. The composition of claim 213 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Coniothyrium microbes, for example 1-20 different Coniothyrium microbes, preferably 1 to 16 different Coniothyrium microbes, more preferably 1 to 10 different Coniothyrium microbes, even more preferably 2 to 10 different Coniothyrium microbes.
215. The composition of claim 213 or 214 wherein the Coniothyrium microbes comprise Coniothyrium minitans.
216. The composition of any one of claims 205 through 215 comprising one or more Lecanicillium microbes.
217. The composition of claim 216 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Lecanicillium microbes, for example 1-20 different Lecanicillium microbes, preferably 1 to 16 different Lecanicillium microbes, more preferably 1 to 10 different Lecanicillium microbes, even more preferably 2 to 10 different Lecanicillium microbes.Docket No. OATH-015-PCT2 218. The composition of any one of claims 205 through 217 comprising one or more Paecilomyces microbes.
219. The composition of claim 218 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Paecilomyces microbes, for example 1-20 different Paecilomyces microbes, preferably 1 to 16 different Paecilomyces microbes, more preferably 1 to 10 different Paecilomyces microbes, even more preferably 2 to 10 different Paecilomyces microbes.
220. The composition of claim 219 wherein the Paecilomyces microbes comprise Paecilomyces lilacinus.
221. The composition of any one of claims 205 through 220 comprising one or more Penicillium microbes.
222. The composition of claim 221 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Penicillium microbes, for example 1-20 different Penicillium microbes, preferably 1 to 16 different Penicillium microbes, more preferably 1 to 10 different Penicillium microbes, even more preferably 2 to 10 different Penicillium microbes.
223. The composition of claim 221 or 222 wherein the Penicillium microbes comprise Penicillium bilaiae.
224. The composition of any one of claims 221 through 223 wherein the Penicillium microbes comprise Penicillium bilaii.
225. The composition of any one of claims 221 through 224 wherein the Penicillium microbes comprise Penicillium oxalicum.
226. The composition of any one of claims 205 through 225 comprising one or more Phlebia microbes.
227. The composition of claim 226 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Phlebia microbes, for example 1-20 different Phlebia microbes, preferably 1 to 16 different PhlebiaDocket No. OATH-015-PCT2 microbes, more preferably 1 to 10 different Phlebia microbes, even more preferably 2 to 10 different Phlebia microbes.
228. The composition of claim 226 or 227 wherein the Phlebia microbes comprise Phlebia gigantea.
229. The composition of any one of claims 205 through 228 comprising one or more Rhizopogon microbes.
230. The composition of claim 229 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Rhizopogon microbes, for example 1-20 different Rhizopogon microbes, preferably 1 to 16 different Rhizopogon microbes, more preferably 1 to 10 different Rhizopogon microbes, even more preferably 2 to 10 different Rhizopogon microbes.
231. The composition of claim 229 or 230 wherein the Rhizopogon microbes comprise Rhizopogon amylopogon.
232. The composition of any one of claims 229 through 231 wherein the Rhizopogon microbes comprise Rhizopogon fulvigleba.
233. The composition of any one of claims 229 through 232 wherein the Rhizopogon microbes comprise Rhizopogon luteolus.
234. The composition of any one of claims 229 through 233 wherein the Rhizopogon microbes comprise Rhizopogon villosullus.
235. The composition of any one of claims 205 through 234 comprising one or more Scleroderma microbes.
236. The composition of claim 235 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Scleroderma microbes, for example 1-20 different Scleroderma microbes, preferably 1 to 16 different Scleroderma microbes, more preferably 1 to 10 different Scleroderma microbes, even more preferably 2 to 10 different Scleroderma microbes.
237. The composition of claim 235 or 236 wherein the Scleroderma microbes comprise Scleroderma cepa.Docket No. OATH-015-PCT2 238. The composition of any one of claims 235 through 237 wherein the Scleroderma microbes comprise Scleroderma citrinum.
239. The composition of any one of claims 205 through 238 comprising one or more Sebacina microbes.
240. The composition of claim 239 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Sebacina microbes, for example 1-20 different Sebacina microbes, preferably 1 to 16 different Sebacina microbes, more preferably 1 to 10 different Sebacina microbes, even more preferably 2 to 10 different Sebacina microbes.
241. The composition of claim 239 or 240 wherien the Sebacina microbes comprise Sebacina vermifera.
242. The composition of any one of claims 203 through 241 wherein the fungal microbes comprise endomycorrhizal fungi.
243. The composition of claim 242 wherein the fungi comprise fungi of one or more of the genera Alternaria, Beauveria, Cladiosporium, Claroideoglomus, Funneliformis, Glomus, Laccaria, Lecanicillium, Metarhizium, Piriformospora, Pisolithus, Rhizophagus, Serendipita, Talaromyces, and / or Trichoderma.
244. The composition of claim 242 or 243 comprising one or more Alternaria microbes.
245. The composition of claim 244 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Alternaria microbes, for example 1-20 different Alternaria microbes, preferably 1 to 16 different Alternaria microbes, more preferably 1 to 10 different Alternaria microbes, even more preferably 2 to 10 different Alternaria microbes.
246. The composition of claim 244 or 245 wherein the Alternaria microbes comprise Alternaria alternata.
247. The composition of any one of claims 242 through 246 comprising one or more Beauveria microbes.Docket No. OATH-015-PCT2 248. The composition of claim 247 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Beauveria microbes, for example 1-20 different Beauveria microbes, preferably 1 to 16 different Beauveria microbes, more preferably 1 to 10 different Beauveria microbes, even more preferably 2 to 10 different Beauveria microbes.
249. The composition of claim 247 or 248 wherein the Beauveria microbes comprise Beauveria bassiana.
250. The composition of any one of claims 242 through 249 comprising one or more Cladiosporium microbes.
251. The composition of claim 250 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Cladiosporium microbes, for example 1-20 different Cladiosporium microbes, preferably 1 to 16 different Cladiosporium microbes, more preferably 1 to 10 different Cladiosporium microbes, even more preferably 2 to 10 different Cladiosporium microbes.
252. The composition of claim 250 or 251 wherein the Cladiosporium microbes comprise Cladiosporium tenuissimum.
253. The composition of any one of claims 242 through 252 comprising one or more Claroideoglomus microbes.
254. The composition of claim 253 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Claroideoglomus microbes, for example 1-20 different Claroideoglomus microbes, preferably 1 to 16 different Claroideoglomus microbes, more preferably 1 to 10 different Claroideoglomus microbes, even more preferably 2 to 10 different Claroideoglomus microbes.
255. The composition of claim 253 or 254 wherein the Claroideoglomus microbes comprise Claroideoglomus etunicatum.
256. The composition of any one of claims 242 through 255 comprising one or more Funneliformis microbes.Docket No. OATH-015-PCT2 257. The composition of claim 256 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Funneliformis microbes, for example 1-20 different Funneliformis microbes, preferably 1 to 16 different Funneliformis microbes, more preferably 1 to 10 different Funneliformis microbes, even more preferably 2 to 10 different Funneliformis microbes.
258. The comprosition of claim 256 or 257 wherein the Funneliformis microbes comprise Funneliformis mosseae.
259. The composition of any one of claims 242 through 258 comprising one or more Glomus microbes.
260. The composition of claim 259 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Glomus microbes, for example 1-20 different Glomus microbes, preferably 1 to 16 different Glomus microbes, more preferably 1 to 10 different Glomus microbes, even more preferably 2 to 10 different Glomus microbes.
261. The composition of claim 259 or 260 wherein the Glomus microbes comprise Glomus aggregatum.
262. The composition of any one of claims 259 through 261 wherein the Glomus microbes comprise Glomus etunicatum.
263. The composition of any one of claims 259 through 262 wherein the Glomus microbes comprise Glomus intraradices.
264. The composition of any one of claims 259 through 263 wherein the Glomus microbes comprise Glomus mosseae.
265. The composition of any one of claims 242 through 264 comprising one or more Laccaria microbes.
266. The composition of claim 265 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Laccaria microbes, for example 1-20 different Laccaria microbes, preferably 1 to 16 differentDocket No. OATH-015-PCT2 Laccaria microbes, more preferably 1 to 10 different Laccaria microbes, even more preferably 2 to 10 different Laccaria microbes.
267. The composition of claim 265 or 266 wherein the Laccaria microbes comprise Laccaria laccata.
268. The composition of any one of claims 242 through 267 comprising one or more Lecanicillium microbes.
269. The composition of claim 268 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Lecanicillium microbes, for example 1-20 different Lecanicillium microbes, preferably 1 to 16 different Lecanicillium microbes, more preferably 1 to 10 different Lecanicillium microbes, even more preferably 2 to 10 different Lecanicillium microbes.
270. The composition of any one of claims 242 through 269 comprising one or more Metarhizium microbes.
271. The composition of claim 270 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Metarhizium microbes, for example 1-20 different Metarhizium microbes, preferably 1 to 16 different Metarhizium microbes, more preferably 1 to 10 different Metarhizium microbes, even more preferably 2 to 10 different Metarhizium microbes.
272. The composition of claim 270 or 271 wherein the Metarhizium microbes comprise Metarhizium anisopliae.
273. The composition of any one of claims 242 through 272 comprising one or more Piriformospora microbes.
274. The composition of claim 273 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Piriformospora microbes, for example 1-20 different Piriformospora microbes, preferably 1 to 16 different Piriformospora microbes, more preferably 1 to 10 different Piriformospora microbes, even more preferably 2 to 10 different Piriformospora microbes.Docket No. OATH-015-PCT2 275. The composition of claim 273 or 274 wherein the Piriformospora microbes comprise Piriformospora indica.
276. The composition of any one of claims 242 through 275 comprising one or more Pisolithus microbes.
277. The composition of claim 276 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Pisolithus microbes, for example 1-20 different Pisolithus microbes, preferably 1 to 16 different Pisolithus microbes, more preferably 1 to 10 different Pisolithus microbes, even more preferably 2 to 10 different Pisolithus microbes.
278. The composition of claim 276 or 277 wherein the Pisolithus microbes comprise Pisolithus tinctorius.
279. The composition of any one of claims 242 through 278 comprising one or more Rhizophagus microbes.
280. The composition of claim 279 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Rhizophagus microbes, for example 1-20 different Rhizophagus microbes, preferably 1 to 16 different Rhizophagus microbes, more preferably 1 to 10 different Rhizophagus microbes, even more preferably 2 to 10 different Rhizophagus microbes.
281. The composition of claim 279 or 280 wherein the Rhizophagus microbes comprise Rhizophagus intraradices.
282. The composition of any one of claims 279 through 281 wherein the Rhizophagus microbes comprise Rhizophagus irregularis.
283. The composition of any one of claims 242 through 282 comprising one or more Serendipita microbes.
284. The composition of claim 283 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Serendipita microbes, for example 1-20 different Serendipita microbes, preferably 1 to 16 differentDocket No. OATH-015-PCT2 Serendipita microbes, more preferably 1 to 10 different Serendipita microbes, even more preferably 2 to 10 different Serendipita microbes.
285. The composition of claim 283 or 284 wherein the Serendipita microbes comprise Serendipita indica.
286. The composition of any one of claims 242 through 285 comprising one or more Talaromyces microbes.
287. The composition of claim 286 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Talaromyces microbes, for example 1-20 different Talaromyces microbes, preferably 1 to 16 different Talaromyces microbes, more preferably 1 to 10 different Talaromyces microbes, even more preferably 2 to 10 different Talaromyces microbes.
288. The composition of claim 286 or 287 wherein the Talaroyces microbes comprise Talaromyces flavus.
289. The composition of any one of claims 242 through 288 comprising one or more Trichoderma microbes.
290. The composition of claim 289 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Trichoderma microbes, for example 1-20 different Trichoderma microbes, preferably 1 to 16 different Trichoderma microbes, more preferably 1 to 10 different Trichoderma microbes, even more preferably 2 to 10 different Trichoderma microbes.
291. The composition of claim 289 or 290 wherein the Trichoderma microbes comprise Trichoderma asperellum.
292. The composition of any one of claims 289 through 291 wherein the Trichoderma microbes comprise Trichoderma atrobrunneum.
293. The composition of any one of claims 289 through 292 wherein the Trichoderma microbes comprise Trichoderma atroviride.
294. The composition of any one of claims 289 through 293 wherein the Trichoderma microbes comprise Trichoderma harzianum.Docket No. OATH-015-PCT2 295. The composition of any one of claims 289 through 294 wherein the Trichoderma microbes comprise Trichoderma viride.
296. The composition of any one of claims 242 through 295 comprising one or more Mucor microbes.
297. The composition of claim 296 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Mucor microbes, for example 1-20 different Mucor microbes, preferably 1 to 16 different Mucor microbes, more preferably 1 to 10 different Mucor microbes, even more preferably 2 to 10 different Mucor microbes.
298. The composition of claim 296 or 297 wherein the Mucor microbes comprise Mucor circinelloides.
299. The composition of any one of claims 242 through 298 comprising one or more Phanerochaete microbes.
300. The composition of claim 299 comprising at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, or 18 and / or not more than 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 14, 16, 18, or 20 different Phanerochaete microbes, for example 1-20 different Phanerochaete microbes, preferably 1 to 16 different Phanerochaete microbes, more preferably 1 to 10 different Phanerochaete microbes, even more preferably 2 to 10 different Phanerochaete microbes.
301. The composition of claim 299 or 300 wherein the Phanerochaete microbes comprise Phanerochaete chrysosporium.
302. The composition of any one of claims 242 through 301 wherein two or more of the fungal microbes are heterologous to each other.
303. A composition comprising a plurality of particles, wherein each particle comprises a composition of any one of claims 1 through 302 304. A delayed release composition comprising a plurality of particles, wherein the particles comprises one or more microbes, and wherein the plurality of particles comprises a first population of particles wherein each particle of the first population comprises one or more first components configured to confine at least a portion of the microbes to the particles, andDocket No. OATH-015-PCT2 wherein the one or more first components are such that the one or more first components lose sufficient integrity upon exposure to one or more environmental triggers that at least a portion of the confined microbes are released from the particles.
305. The composition of any one of the previous claims further comprising one or more agriculturally acceptable adjuvants.
306. The composition of claim 305 wherein the adjuvant comprises polyethylene glycol.
307. The composition of claim 305 wherein the adjuvant consists essentially of glucose.
308. A population of seeds, seedlings, or other plant propagules comprising at least 10, 12, 15, 17, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, or and / or not more than 12, 15, 17, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, or 120 different microbes, wherein at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 95%, or 100%, of the microbes are heterologous to the seeds, seedlings, or other plant propagules, wherein at least a portion of the microbes of the composition are associated with the seeds, seedlings, or other plant propagules.
309. The population of claim 308 wherein the microbes comprise microbes of a composition of any one of claims 1 through 302 310. A composition comprising soil in which plants are growing or have grown wherein the soil comprises at least 1, 2, 5, 10, 15, 20, 30, 35, 40, 45, 50, 60, 70, 80, 90, or 100 and / or not more than 2, 5, 10, 15, 20, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, or 150 different microbes that are heterologous to the soil and / or plants, for example, at least 1, preferably at least 5, more preferably at least 10, even more preferably at least 15, yet more preferably at least 25, still more preferably at least 40, or 1-150, preferably 1-100, more preferably 2-80, even more preferably 2.
311. The composition of claim 310 wherein the microbes comprise microbes of a composition of any one of claims 1 through 302 312. A composition comprising soil in which plants are growing or have grown wherein the soil comprises at least 1, 2, 5, 10, 15, 20, 30, 35, 40, or 45 different microbes of a composition of any one of claims 1 through 302Docket No. OATH-015-PCT2 313. A plant or plant part comprising one or more microbes of the composition of any one of any one of claims 1 through 302 disposed on or in the plant or plant part.
314. A composition comprising a population of plants wherein the plants or part of the plants comprises the microbe or microbes of the composition of any one of claims 1 through 302 disposed on or in the plant or plant part.
315. A method comprising applying the composition of any one of claims 1 through 302, or a derivative thereof, to a plant, or a part thereof, and / or to soil or other growth medium in which the plant grows or will grow, in a first application.
316. A method comprising applying a composition comprising a plurality of microbes to an area comprising soil by aerial application.
317. A method comprising applying to a plant growth medium (a) a plurality of microbes, and (b) one or more macronutrients, (c) one or more micronutrients, and / or (d) one or more carbon sources, wherein a composition and / or effective amount of (a), (b), (c) and / or (d) is determined at least in part by one or more results of a first test on one or more samples comprising growth medium to which the plurality of microbes is to be applied or has been applied and / or a component of a plant or plants growing in the growth medium.
318. A method comprising contacting a surface of a seed, seedling, or other plant propagule with a composition of any one of claims 1 through 302, or a derivative produced from the composition.
319. A method comprising contacting surfaces of a populations of seeds, seedlings, or other plant propagules with a composition of any one of claims 1 through 302, or a derivative produced from the composition.
320. Use of the composition of any one of claims 1 through 302 in reducing methane production in soil in which plants grow compared to before use of the composition.Docket No. OATH-015-PCT2 321. Use of the composition of any one of claims 1 through 302 in coating a surface of seeds, seedlings, or other plant propagules with one or more microbes of the composition.
322. Use of the composition of any one of claims 1 through 302 in in increasing beneficial organisms in soil in which plants grow and to which composition applied, compared to without.
323. Use of the composition of any one of claims 1 through 302 in transitioning to organic farming in 3 years or less, or 2 years or less, or 1.5 years or less.
324. Use of the composition of any one of claims 1 through 302 in improving soil density, soil water holding capacity, soil water retention capacity, in soil in which plants grow, compared to without.
325. Use of the composition of any one of claims 1 through 302 in increasing mycorrhizal fungi on or in roots of plant, compared to a reference plant 326. Use of the composition of any one of claims 1 through 302 in preventing or reducing flooding.
327. Use of the composition of any one of claims 1 through 302 in decreasing use of synthetic fertilizer, herbicide, and / or pesticide on plants or soil in which plants grow, compared to without.
328. Use of the composition of any one of claims 1 through 302 in increasing nutrient uptake by a plant grown in soil to which composition applied compared to without.
329. Use of the composition of any one of claims 1 through 302 in decreasing uptake of one or more toxins, e.g., heavy metals, by plants grown in soil comprising composition.
330. Use of the composition of any one of claims 1 through 302 in increasing plant nutrient density of a plant grown in soil to which composition applied compared to without.Docket No. OATH-015-PCT2 331. Use of the composition of any one of claims 1 through 302 in reducing carbon footprint for raising livestock.
332. Use of the composition of any one of claims 1 through 302 in increasing oil production of a plant not cannabis.
333. Use of the composition of any one of claims 1 through 302 in regenerating degraded soil.
334. A method of growing one or more microbes comprising inoculating a heterologous medium with a first population of microbes comprising microbes of the composition of a any one of claims 1 through 302 and providing suitable conditions for one or more microbes to grow.
335. A composition comprising a pure population of microbes of the composition of any one of claims 1 through 302 and a heterologous growth medium.
336. A composition consisting essentially of a pure population of of microbes of the composition of any one of claims 1 through 302 and a heterologous growth medium.
337. A method for enhancing a rate at which atmospheric carbon is converted to carbon products in soil in which plants are grown and / or in the plants comprising applying a composition comprising one or more microbes to the plants and / or soil,in a first application, wherein the rate is greater than that for reference soil and / or plants in which the plants are grown without the composition.
338. The method of claim 337 wherein the composition comprises a composition of any one of claims 1 through 302.
339. A method of increasing soil carbon content comprising allowing plants to grow in the soil for a period of time, wherein the soil and / or plants have been treated by (i) applying to the soil in which the plants are grown or will be grown, and / or the plants, a first application of a composition comprising a plurality of different microbes; andDocket No. OATH-015-PCT2 (ii) providing suitable conditions for the plants to grow in the soil for the period of time; thereby increasing soil carbon content.
340. The method of claim 339 wherein the composition comprises a composition of any one of claims 1 through 302.
341. A composition comprising soil and plants growing in the soil, wherein the plants comprise parts in the soil, e.g., roots, and wherein the soil and plant parts comprise (i) a carbon content of at least 0.5, 2, 3, 5, 7, 10, 12, 15, 20, 25, 30, or 35 kg / m3and / or not more than 0.5, 2, 3, 5, 7, 10, 12, 15, 20, 25, 30, 35 or 40 kg / m3, for example 0.5-40, preferably 2- 40, more preferably 5-40, even more preferably 7-35, yet more preferably 7-30, still more preferably 7-25 kg / m3of soil; and (ii) at least 1, 2, 5, 10, 15, 20, 30, 35, 40, or microbes that are heterologous to the soil and / or plants, for example, at least 1, preferably at least 5, more preferably at least 10, even more preferably at least 15, yet more preferably at least 25, still more preferably at least 40.
342. A method of reducing soil methane production comprising allowing plants to grow in the soil for a period of time, wherein the soil and / or plants have been treated by (i) applying to the soil in which the plants are grown or will be grown, the plants, and / or seeds of the plants a first application of a composition comprising a plurality of different microbes; and (ii) providing conditions for the plants to grow in the soil;thereby increasing decreasing soil methane production.
343. The method of claim 342 wherein the composition comprises a composition of any one of claims 1 through 302.
344. A method of transferring atmospheric carbon to soil in which plants grow comprising (i) applying a composition comprising a plurality of different microbes to the soil, plants, plant parts, and / or seed of the plants, wherein the composition is applied over an area of at least 1000, 10,000, 100,000, 1M, 10M, 50M, 100M, 250M, or 500M hectares and / or not more than 10,000, 100,000, 1M, 10M, 50M, 100M, 250M, 500M, or 1B hectares, for example 1000 to 1B, preferably 10,000 to 1B, more preferably 100,000 to 1B, even more preferably 1M to 1B hectares, yet more preferably 10M to 1B hectares, still more preferably 50M to 1B hectares; andDocket No. OATH-015-PCT2 (ii) providing conditions suitable for growth of the plants for a period of time, thereby transferring atmospheric carbon to the soil to increase carbon content of the soil.
345. The method of claim 344 wherein the composition comprises a composition of any one of claims 1 through 302.
346. A method of reducing an overall carbon footprint for raising domesticated animals comprising feeding the animals, and / or allowing the animals to feed on, plants or parts of plants, wherein the plants have been or are growing in soil to which has been applied a composition comprising a plurality of different microbes, wherein the composition (i) increases carbon dioxide transfer from atmosphere to soil thus increasing carbon content of the soil, compared to without the composition; (ii) increases biomass of the plants in a given area, compared to without the composition, so that carbon produced per unit biomass of plants is decreased; and (iii) increases nutrient density of the plants, compared to without the composition, so that less biomass of plants is used to produce a given amount of increase in economically valuable attribute of the domesticated animals or a combination thereof, thus decreasing overall carbon footprint for raising the domesticated animals.
347. The method of claim 346 wherein the composition comprises a composition of any one of claims 1 through 302.
348. A method of reducing water use for growth and / or maintenance of plants in soil comprising allowing the plants to grow and / or be maintained in the soil for a period of time, wherein the soil and / or plants have been treated by (i) applying to the soil, the plants, a first application of a composition comprising a plurality of different microbes or a derivative thereof; and (ii) providing suitable conditions for the plants to grow and / or be maintained in the soil for a period of time,; thereby decreasing the amount of water necessary to produce a given amount of biomass from the plants and / or to maintain the plants in a desired state, compared to the amount of waterDocket No. OATH-015-PCT2 necessary to produce the biomass and / or maintain the plants in a desired state for reference soil and plants, for the period of time.
349. The method of claim 348 wherein the composition comprises a composition of any one of claims 1 through 302.
350. A method of increasing water holding capacity of soil in which plants are grown comprising allowing the plants to grow in the soil for a period of time, wherein the soil and / or plants have been treated by (i) applying to the soil in which the plants are grown or will be grown and / or the plants, a first application of a composition comprising a plurality of different microbes or a derivative thereof; and (ii) providing suitable conditions for the plants to grow in the soil for a period of time; thereby increasing the water holding capacity of the soil, for example, WHC compared to reference soil and plants.
351. The method of claim 350 wherein the composition comprises a composition of any one of claims 1 through 302.
352. A composition comprising soil in which plants are growing or have grown wherein the soil comprises (i) a water holding capacity of at least 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70% wt / wt and / or not more than 25, 30, 35, 40, 45, 50, 55, 60, 65, or 70% wt / wt, for example, 20-70%, preferably 25-65%, more preferably 25-55%, yet more preferably 30-65%, still more preferably 35-65%, yet still more preferably 35-55%; and (ii) at least 1, 2, 5, 10, 15, 20, 30, 35, 40, 45, 50, 70, or 100, and / or not more than 2, 5, 10, 15, 20, 30, 35, 40, 45, 50, 70, 100, or 150 different microbes that are heterologous to the soil and / or plants, for example, at least 1, preferably at least 5, more preferably at least 10, even more preferably at least 15, yet more preferably at least 25, still more preferably at least 40, or preferably 1-150, more preferably 1-100, even more preferably 1-70, yet more preferably 1-50.
353. The composition of claim 352 wherein the microbes comprise microbes of a composition of any one of claims 1 through 302.Docket No. OATH-015-PCT2 354. A composition comprising soil in which plants are growing or have grown wherein the soil comprises (i) an average density of at least 0.8, 0.9, 1.0, 1.05, 1.10, 1.15, 1.20, 1.25, 1.30, 1.35, 1.40, 1.45, 1.50, 1.55, 1.60, 1.65, or 1.70 and / or not more than 0.9, 1.0, 1.05, 1.10, 1.15, 1.20, 1.25, 1.30, 1.35, 1.40, 1.45, 1.50, 1.55, 1.60, 1.65, 1.70, 1.75, 1.80, 1.85, 1.90, or 1.95, for example, 1.0-1.90, preferably 1.05 to 1.85, more preferably 1.10 to 1.50, even more preferably 1.20-1.75, yet more preferably 1.35-1.70, still more preferably 1.55-1.85 g / cm3 dry weight of soil; and (ii) at least 1, 2, 5, 10, 15, 20, 30, 35, 40, 45, 50, 70, or 100, and / or not more than 2, 5, 10, 15, 20, 30, 35, 40, 45, 50, 70, 100, or 150 different microbes that are heterologous to the soil and / or plants, for example, at least 1, preferably at least 5, more preferably at least 10, even more preferably at least 15, yet more preferably at least 25, still more preferably at least 40 or preferably 1-150, more preferably 1-100, even more preferably 1-70, yet more preferably 1-50.
355. A method of modulating, e.g., improving, one or more characteristics of soil in which plants grow and / or are maintained, comprising allowing the plants to grow in the soil for a period of time, wherein the soil and / or plants have been treated by (i) applying to the soil in which the plants are grown or will be grown and / or the plants, a first application of a composition comprising a plurality of different microbes; and (ii) providing suitable conditions for the plants to grow in the soil for the period of time thereby modulating, e.g,, improving the one or more characteristics compared to reference soil and plants.
356. The method of claim 355 wherein the composition comprises a composition of any one of claims 1 through 302.
357. A method of regenerating vegetation in a soil that has been partially or completely depleted of one or more types of vegetation and / or has reduced capacity to grow and / or maintain new vegetation, comprising (i) applying to the soil, plants growing or to be grown in the soil, and / or seeds of the plants a first application of a composition comprising a plurality of different microbes; and (ii) providing suitable conditions for the plants to grow in the soil for a period of time.
358. The method of claim 357 wherein the composition comprises a composition of any one of claims 1 through 302.Docket No. OATH-015-PCT2 359. A method of repairing damaged or degraded soil comprising (i) applying to the soil a first application of a composition comprising a plurality of different microbes; and (ii) providing suitable conditions for plants to grow in the soil for the period of time.
360. The method of claim 358 wherein the composition comprises a composition of any one of claims 1 through 302.
361. A method for decreasing or eliminating flooding on land that has experienced or is likely to experience flooding, wherein soil of the land has grown, is growing, or will grow plants, comprising (i) applying to the soil, the plants, and / or seeds of the plants a first application of a composition comprising a plurality of different microbes; and (ii) providing suitable conditions for the plants to grow in the soil for the period of time; thereby decreasing or eliminating flooding on the land.
362. The method of claim 361 wherein the composition comprises a composition of any one of claims 1 through 302.
363. A method for increasing density of one or more nutrients in plants or plant parts, e.g., plants or plant parts to be consumed by animals, comprising (i) applying a composition comprising a plurality of different microbes to the soil in which the plants are grown or will be grown and / orthe plants, ; and (ii) providing conditions suitable for growth of the plants for a period of time, e.g., a period of time sufficient for the plants or plant parts to be suitable for consumption by the animals; thereby increasing density of the one or nutrients in the plants or plant parts compared to reference plants or plant parts.
364. The method of claim 363 wherein the composition comprises a composition of any one of claims 1 through 302.
365. A method for increasing uptake of one or more plant nutrients by plants grown in soil comprisingDocket No. OATH-015-PCT2 (i) applying a composition comprising a plurality of different microbes to the soil in which the plants are grown or will be grown and / or the plants; and (ii) providing conditions suitable for growth of the plants and proliferation and / or other actions of the microbes for a suitable duration for plant nutrient uptake to increase; whereby plant nutrient uptake by the plants is increased.
366. The method of claim 365 wherein the composition comprises a composition of any one of claims 1 through 302.
367. A method for converting one or more plant nutrients in soil in which plants are grown from non-bioavailable to bioavailable form (i) applying a composition comprising a plurality of different microbes to the soil in which the plants are grown or will be grown and / orthe plants; and (ii) providing conditions suitable for growth of the plants and proliferation and / or other actions of the microbes for a duration sufficient for the one or more plant nutrients in the soil to convert from non-bioavailable to bioavailable form; whereby the one or more plant nutrients are converted from non-bioavailable to bioavailable form.
368. The method of claim 367 wherein the composition comprises a composition of any one of claims 1 through 302.
369. A composition comprising a plant comprising (i) an above-ground portion; and (ii) a below-ground portion, wherein the below-ground portion comprises roots of the plant and soil associated with the roots, wherein the below-ground portion comprises (a) one or more microbes heterologous to the soil and / or plant; and (b) a concentration of bio-available nutrient that is greater than reference plant and soil.
370. A composition comprising a plant, e.g. wherein the plant or part of the plant is for animal consumption, and soil in which the plant is growing, wherein (i) the soil and / or roots of the plant in the soil comprise one or more microbes heterologous to the soil and / or plant; and (ii) the plant or plant part comprises a density of one or more nutrients, e.g., one or more nutrients for the animal, that is greater than reference plant or plant partDocket No. OATH-015-PCT2 371. A method to convert non-organic agricultural practice to organic agricultural practice comprising (i) applying a composition comprising a plurality of microbes to plants, and / or to soil in which the plants grow or will grow, in a first application at a first time, wherein up to the first time the plants and soil were subject to non-organic farming practice; (ii) providing conditions suitable for growth of the plants and proliferation and other actions of the microbes for a period of time wherein after the period of time the non-organic agricultural practice has been converted to an organic agricultural practice.
372. The method of claim 371 wherein the composition comprises a composition of any one of claims 1 through 302.
373. A method for reducing concentration of a toxin in a soil, reducing uptake of the toxin by plants growing in the soil, and / or reducing a concentration of the toxin in the plants or plant parts, comprising (i) applying a composition comprising a plurality of microbes to the plants, or parts thereof, and / or to the soil, in a first application; (ii) providing conditions suitable for growth of the plants and proliferation and other actions of the microbes for a period of time wherein after the period of time concentration of the toxin in the soil, uptake of the toxin by plants growing in the soil, and / or concentration of the toxin in the plants or plant parts is reduced compared to before the first application.
374. The method of claim 373 wherein the composition comprises a composition of any one of claims 1 through 302.
375. A method of improving one or more characteristics of a tree rooted in soil comprising (i) providing one or more nutrients to the soil in which the tree is rooted; and (ii) providing a composition comprising at least 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, or 150 and / or not more than 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60,Docket No. OATH-015-PCT2 70, 80, 90, 100, 120, 150, or 200 different microbes to the soil and / or the tree in a first application.
376. The method of claim 375 wherein the composition comprises a composition of any one of claims 1 through 302.
377. A method of revitalizing a dormant tree comprising applying to the soil in which the tree is rooted and / or the tree a composition comprising at least 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, or 150 and / or not more than 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, 150, or 200 different microbes to the soil in wherein the microbial composition the tree is rooted in a first application and allowing the tree to grow for a duration of time.
378. The method of claim 377 wherein the composition comprises a composition of any one of claims 1 through 302.
379. A kit comprising (i) a composition comprising at least 1, 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, or 150 and / or not more than 2, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90, 100, 120, 150, or 200 different microbes in a first package; and (ii) a composition comprising at least 1, 2, 3, 4, 5, 7, 10, 15, 20, 30, 40, 50, 100, or 200 and / or not more than 2, 3, 4, 5, 7, 10, 15, 20, 30, 40, 50, 100, 200 or 500 nutrients in a second package, different from the first.
380. The kit of claim 379 wherein the composition comprising microbes comprises a composition of any one of claims 1 through 302.
381. A method comprising applying a composition comprising bacterial microbes and fungal microbes to a plurality of tree seedlings and / or growth medium in which the seedlings are located.
382. The method of claim 381 wherein the composition comprising bacterial microbes and fungal microbes comprises a composition of any one of claims 203 through 302.