Dish with multiple wells

Abstract

This dish comprises multiple wells; and a groove part at a position on an outer side of the well of the dish, the groove part accommodating part of a bar-shaped member used for a process of handling a fertilized egg on the dish. This groove part has both ends that are open ends, the groove part has an inclined structure that accommodates the bar-shaped member in a state where the bar-shaped member is inclined with respect to a horizontal plane in a formation direction of the groove part, and a lower part height that is a height of the open end having a lower height from a dish bottom surface of a bottom part of the groove part among the open ends is higher than a height of a bottom part of one of the multiple wells.　Consequently, it is easy to handle the bar-shaped member when the dish is used together with other members.

Description

DISH WITH MULTIPLE WELLSCross-Reference To Related Application

[0001] The present application claims priority from Japanese application P2023-186491 filed on October 31, 2023, the content of which is hereby incorporated by reference into this application.Background

[0002] 1. Field The present disclosure relates to a dish with multiple wells.

[0003] 2. Related Art When handling eggs, for example when cleaning of eggs, a freezing treatment of fertilized eggs, or a thawing treatment of frozen fertilized eggs, dishes may be used.　Such dishes include a known dish with multiple wells as disclosed in, for example, JP 2017-63744A.　When work is performed using a dish with multiple wells, for example, in a freezing treatment of fertilized eggs, an ES solution for dehydration is filled in one of the multiple wells, a VS solution for vitrification is filled in another one of the multiple wells, and following work is performed under a microscope.　First, a fertilized egg is transferred to a well filled with the ES solution, and is then moved to a well filled with the VS solution after a certain period of time passes; furthermore, the egg is placed on a device used for freezing fertilized eggs, hereinafter referred to as the freezing device. This freezing device is put in a container filled with liquid nitrogen together with its casing, and the fertilized egg is cryopreserved.

[0004] This dish with the multiple wells is generalized to some degree, and is used for various purposes.　For example, in a field of embryo culture, the dishes are used for the above-described freezing treatment of the fertilized eggs or embryos and, in addition, for a thawing treatment of cryopreserved fertilized eggs or embryos, a treatment of collecting eggs from an antral fluid at a time of sampling, and the like.　Furthermore, such a dish may also be used for cryopreservation of sperms or the like as described in JP 2001-328901A.

[0005] However, the inventors have noticed that some problems may occur at a time of a process of handling eggs under a microscope in a field of fertility treatment or the like.　One of the problems is that it may be difficult to handle a bar-shaped member that is used together with such a dish.　For example, in a process of handling the dish under a microscope, it is forced to adjust the focus of the microscope due to a difference between a position of an egg on the dish in a height direction and a height of the bar-shaped member used for treatments such as a freezing device.　Another one of the problems is that a recess part provided between the wells is difficult to use as a result of giving importance to versatility.Summary

[0006] The present disclosure can be implemented as following aspects or application embodiments.

[0007] (1) One of embodiments of the present disclosure is a dish.　This dish comprising: multiple wells; and a groove part at a position on an outer side of the well of the dish, the groove part accommodating part of a bar-shaped member used for a process of handling a fertilized egg on the dish, wherein the groove part has both ends that are open ends, the groove part has an inclined structure that accommodates the bar-shaped member in a state where the bar-shaped member is inclined with respect to a horizontal plane in a formation direction of the groove part, and a lower part height that is a height of the open end having a lower height from a dish bottom surface of a bottom part of the groove part among the open ends is higher than a height of a bottom part of one of the multiple wells.　Thus, it is possible to accommodate the bar-shaped member in this groove part, and handle the accommodated bar-shaped member together with the dish.

[0008] (2) Another embodiment of the present disclosure is a rectangular dish including multiple wells.　According to this rectangular dish, the number of the multiple wells may be four or six, the four wells may be disposed at four corners of a square, a recess part that is a recess of a cross shape may be provided between the four wells, the recess part of the cross shape may be the lowest and the deepest at a center part, and the recess part extending in the cross shape around the center part may include a slope that becomes shallower toward an end part.　By so doing, it is possible to reduce the volume of the recess part of the cross shape provided between the wells.　Consequently, it is possible to reduce a usage of a liquid such as a reagent or a cleaning liquid for work or a culture solution that is injected into this recess part to use.　This is because, although a predetermined depth (water depth) of the liquid used for work is required, the water depth of the entire recess part of the cross shape may not be the predetermined depth, and it is sufficient that part of the water depth satisfies the predetermined depth.　This rectangular dish includes a slope whose center part is deep and that becomes shallower toward end parts, so that it is also easy to perform work that requires that a distal end of a pipette reaches a deep part of the water depth.　Furthermore, although the water depth having the depth corresponding to the end of the recess part makes it difficult to find fertilized eggs, embryos, cell debris, or the like that have entered the end of the recess part, the slope that becomes shallower toward the end parts hardly causes this difficulty.

[0009] Fig. 1 is a perspective view illustrating an outer shape of a rectangular dish according to an embodiment;Fig. 2 is a perspective view illustrating outer shapes of a dish body and a lid body of the rectangular dish according to the embodiment;Fig. 3 is a plan view of the lid body according to the embodiment;Fig. 4 is an explanatory view collectively illustrating right and left side views and a bottom view of the lid body according to the embodiment;Fig. 5 is a plan view of a rectangular dish body according to the embodiment;Fig. 6 is an explanatory view collectively illustrating right and left side views of the rectangular dish body according to the embodiment;Fig. 7 is an explanatory view collectively illustrating an end surface of a cut surface of each part in Fig. 5;Fig. 8 is an explanatory view for describing a positional relationship of a fertilized egg in a well of a dish put under a microscope;Fig. 9 is a plan view illustrating a state where a freezing device is placed in a groove part of the dish;Fig. 10 is an explanatory view for describing a positional relationship in a case where the freezing device is placed in the groove part of the dish;Fig. 11 is an explanatory view for describing a difference between volumes of recess parts produced by a difference between bottom part shapes of the recess parts; andFig. 12 is an explanatory view for exemplifying the shape of a round dish having three dishes.Detailed Description

[0010] A. First Embodiment: (A1) Schematic Configuration of Dish: As illustrated in Figs. 1 and 2, a dish according to the present embodiment is a rectangular dish 20 in a substantially square shape, and adopts a form obtained by combining a dish body 40 and a lid body 30.　Fig. 1 illustrates a state where the dish body 40 and the lid body 30 are combined, and Fig. 2 illustrates a state where the lid body 30 is detached.　Note that the outer circumferential shape of this rectangular dish 20 has the four corners one of which is chamfered. Then the rectangle dish 20 is formed in an asymmetrical shape in a front / rear direction perpendicular to a direction (right / left direction) in a plane when the rectangular dish 20 is put on a desk or the like.　A normal use position is a position at which a chamfered site 22 is put on a front left of a worker, and upper, lower, right, left, front, and rear directions will be defined as follows in the following description.　A height direction of the rectangular dish 20 will be referred to as a Z direction, the right / left direction in a case where the chamfered part is on the front left will be referred to as a Y direction, and a direction perpendicular to the Z direction and the Y direction will be referred to as an X direction.　These directions are also illustrated under the same definition as appropriate in the other figures.

[0011] The dish body 40 is made of a synthetic resin, and is integrally formed using a mold.　The dish body 40 includes a base part 41 that is a base, and a raised part 43 that is formed thereon.　As illustrated in Figs. 3 and 4, the lid body 30 includes a planar part 31 and a surrounding part 33 that surrounds the outer circumference of the planar part 31 and has a predetermined dimension.　The inner dimension of the surrounding part 33 is made slightly larger than the outer dimension of the raised part 43 of the dish body 40, and the lid body 30 is molded in such a size that the lid body 30 accommodates the raised part 43 inside and covers the base part 41.　A protrusion part 32 is provided at the center of each side of the planar part 31 of the lid body 30.　These protrusion parts 32 are provided to position the rectangular dishes 20 when the rectangular dishes 20 are stacked, prevent the rectangular dish 20 at an upper part from slipping down, or slightly float the planar part 31 from a desk plane when the lid body 30 is turned over and is put on the desk or the like.

[0012] As for joining points of the planar part 31 and the surrounding part 33 of the lid body 30, end parts of the planar part 31 slightly protrude from the outer surfaces of the surrounding part 33 as illustrated in a side view in Fig. 4.　These protruding portions will be referred to as engagement parts 39.　The engagement parts 39 help the worker reliably grip the rectangular dish 20 or the lid body 30 when the worker engages the finger tips with the rectangular dish 20 or the lid body 30 alone to lift when holding the rectangular dish 20 or the lid body 30 alone.　Similar engagement parts 49 are also provided at outer circumferential end parts of the base part 41 of the dish body 40, and help the worker, for example, reliably grip the rectangular dish 20 or the dish body 40 alone when the worker lifts the rectangular dish 20 or the dish body 40 alone.　The engagement parts 49 provided to the dish body 40 are illustrated in Fig. 6 described later.　Protrusion amounts of the engagement parts 39 and the engagement parts 49 are preferably approximately 0.1 to 1.0 mm, are preferably approximately 0.2 to 0.5 mm, and is 0.5 mm in the present embodiment.

[0013] A small protrusion part 36 is formed at the center of each side inside the lid body 30.　Since these protrusion parts 36 are provided, when the lid body 30 covers the dish body 40, the protrusion parts 36 come into contact with the raised part 43 of the dish body 40, and form a slight gap between the lid body 30 and the dish body 40.　Hence, the rectangular dish 20 is not placed in a sealed state, and a space inside the rectangular dish 20 is kept in a state where a gas can be exchanged with an outside.　Note that one of the four corners of the lid body 30 corresponds to the chamfered site 22, and includes a chamfered part 34 that is chamfered at substantially 45 degrees.　This chamfered part 34 corresponds to a chamfered part 44 of the raised part 43 that is formed likewise, and restricts a fitting position of the lid body 30 with respect to the dish body 40.

[0014] The raised part 43 of the dish body 40 includes four circular wells 51, 52, 53, and 54 that are apart from each other and provided at the four corners of the raised part 43.　A recess part 55 is formed at a site of a substantially cross shape surrounded by the four wells 51 to 54.　The shapes in a depth direction of the wells 51 to 54 and the recess part 55 will be described later.　Although the synthetic resin itself for forming the dish body 40 is transparent, a flat upper surface 47 of the raised part 43 has fine protrusions and recesses formed thereon and is semi-transparent to enhance visibility of the interior of the wells 51 to 54.　Hatching is applied to part of the drawings, and the entire upper surface 47 is formed in a so-called frosted glass.　First of all, the upper surface 47 may be transparent.　Furthermore, the recess part 55 may not be provided.　The number of wells may be two or more, and arrangement thereof is also arbitrary.　In a case where five or more wells are provided, the shape of the rectangular dish 20 may be a rectangular shape, and p wells in a column × q wells in a row (p and q represent integers equal to or more than two and, in this regard, p ≠ q) except for a case where the number of wells is an exponentiation of an integer such as nine or 16.

[0015] One of the four corners of the raised part 43 of the dish body 40 is chamfered as described above, and the chamfered part 44 is formed at this one corner.　The chamfered part 44 helps a user of this rectangular dish 20 quickly and reliably recognize the direction of the dish.　Arrangement for placing this chamfered part 44 on the lower left is the normal use position as described above.

[0016] The raised part 43 on an opposite side, that is, an X direction side of the chamfered part 44 of the dish body 40 is provided with a groove part 60.　Details and a function of a configuration of the groove part 60 will be described later.

[0017] (A2) Shapes of Well, Recess, and Groove Part: Fig. 5 is a plan view of the dish body 40.　A right side view (VI-R arrow view) and a left side view (VI-L arrow view) of the dish body 40 are collectively illustrated in Fig. 6.　Furthermore, arrow view direction end views taken along an illustrated line VII-A, line VII-B, and line VII-C are collectively illustrated in Fig. 7.　As illustrated in these drawings, the four wells 51 to 54 have the identical depth, and have bottom parts that are formed flat.　Furthermore, the outer sides of the bottom parts of the wells 51 to 54 are formed slightly higher than the lower end of the base part 41, and are not in direct contact with a placement surface when the dish body 40 is put on the placement surface of the desk or the like.

[0018] The recess part 55 has a substantially cross shape, and includes a center part 57c and four narrow parts 56.　The narrow part 56 is sandwiched between the adjacent wells.　The shape in the depth direction of the recess part 55 is illustrated in the uppermost part in Fig. 7 as the VII-A end view.　As illustrated in Fig. 7, the bottom part 57 of the recess part 55 is moderately curved, and the center part 57c is formed the most deeply.　The narrow part 56 from the center part 57c to the end part 57e forms a slope that gradually becomes shallower toward the end part 57e.　The depth of this center part 57c is approximately 2 / 3 compared to the depths of the wells 51 to 54.　Furthermore, the depth of the recess part 55 is approximately 1 / 2 of the depth of 57c on a line that is close to the end part 57e and passes the center of the adjacent wells.　Note that the shapes of the four slopes formed from the center part 57c to the end parts 57e are identical in this embodiment, yet may be different in each of the X direction and the Y direction, and may all be different shapes.　Furthermore, a shape may not be included in one of the slopes formed in the X direction or the Y direction.　Furthermore, the other slopes than one of the slopes may not be formed.

[0019] The groove part 60 provided on a dish upper side of the dish body 40 is formed by forming, in a groove shape, part of an X direction side of the raised part 43 as illustrated in Figs. 5 and 6, and is a site sandwiched between an outer circumferential wall 63 that is left as an outermost part of one side of the raised part 43, and an upper wall 65 of the raised part 43.　This groove part 60 is provided with an inclined surface 61 that moderately inclines at an angle θ (theta) from the well 51 side to the well 52 side as illustrated in a lowermost part in Fig. 7.　This angle θ (theta) is approximately six degrees in the present embodiment.　Furthermore, an end part height of the inclined surface 61 matches with the height of the raised part 43 at an end part 61L on a Y direction left side, and matches with the height of the base part 41 at an end part 61R on a Y direction right side.　Since the bottom part of each of the wells 51 to 54 is at a position lower than the height of the base part 41, the end part 61R is higher than the bottom part of each of the wells 51 to 54.

[0020] (A3) Use of Groove Part: Fig. 8 illustrates a positional relationship of a fertilized egg in a well of a dish put under a microscope.　The rectangular dish 20 according to the present embodiment is a so-called four-well dish.　The rectangular dish 20 of this type is used in various fields of not only the reproductive medicine, but also the biological science and cell culture, and, here, a freezing treatment of fertilized eggs or embryos using the freezing device as a bar-shaped member will be cited as an example and described.　Although “Cryotop” (registered trademark) made by Kitazato Corporation is used as the freezing device, any device may be used as long as the device is another device such as the bar-shaped member such as “Diamour” (registered trademark) made by Mitsubishi Paper Mills Limited.　For example, an injection pipette or a holding pipette, a forceps (tweezer), or the like may be used.　The worker puts a container of the freezing device, fills the container with liquid nitrogen, and puts the container in a cane to prepare.

[0021] When a fertilized egg or the like is frozen, approximately 0.5 mL of an Equilibration Solution (ES) is dispensed to the well 51 of each well of the rectangular dish 20, and approximately 0.5 mL of a Vitrification Solution (VS) is dispensed to the well 52.　Instead of the well 52, the well 53 may be used.　Furthermore, a cleaning solution for cleaning is injected in the recess part 55.　Then, (usually, a plurality of) fertilized eggs 25 cultured in an embryo culture device (not illustrated) are taken out from dishes for culturing using a pipette, and transferred into the ES of the well 51.　The fertilized eggs 25 immersed in the ES for a predetermined time are transferred into the VS of the well 52 using a pipette.　Fig. 8 illustrates this state.

[0022] In this case, since the bottom surface of the well 52 is substantially flat, the fertilized egg 25 is located at a position of the height of the bottom surface of the well 52, that is, a height WD from a stage 80 on which the dish body 40 is put if the diameter of the fertilized egg 25 is ignored.　Next, work of sucking to the pipette the fertilized egg 25 in the VS, and placing the fertilized egg 25 at the distal end of the freezing device from which a cap (not illustrated) has been detached is performed.　Since the work of transferring the fertilized egg 25 to the well 51 is performed in the visual field of the microscope 100, the focus of the microscope 100, more specifically, a position in the upper / lower direction of an objective lens is adjusted such that a distance FD to the fertilized egg 25 is equal to the focal distance of the microscope 100.

[0023] Immediately before the work of placing the fertilized egg 25 on the freezing device is performed, the freezing device is taken out, and set to the groove part 60 of the dish body 40.　Figs. 9 and 10 illustrate this situation.　As illustrated in Figs. 9 and 10, a freezing device 70 includes a top 71 on which a fertilized egg for freezing (hereinafter, referred to as a frozen egg), and a device body 72 that holds this top 71.　The top 71 is thin and transparent, made of a synthetic resin, and has elasticity.　Conventionally, the worker usually holds the device body 72 of the freezing device 70 by the left hand, brings a portion of this top 71 on which the frozen egg 25 is placed into the visual field of the microscope 100, and holds the frozen egg 25 in a state where the focus is adjusted.　For this purpose, a black mark 75 is generally assigned near the distal end of the top 71.

[0024] By contrast with this, in the present embodiment, the worker sets the freezing device 70 to the groove part 60 of the dish body 40 as illustrated.　At this time, when the device body 72 of the freezing device 70 is fitted to the groove part 60, the inclined surface 61 of the groove part 60 is inclined at the angle θ (theta), and the top 71 of the freezing device 70 approaches the stage 80.　By adjusting the position in an axial direction Y of the freezing device 70, it is also possible to adjust the position in the Z direction, that is, the height direction.　Hence, the position in the Y direction of the freezing device 70 is adjusted to adjust in advance the heights (the positions in the Z direction) of the mark 75 and the vicinity thereof to substantially the same height as the height of the bottom part of the well 52.

[0025] By so doing, when the frozen egg in the VS of the well 52 is sucked to the pipette, and then the dish body 40 is moved such that the vicinity of the mark 75 of the top 71 enters the visual field of the microscope 100, the focus of the microscope 100 matches with the vicinity of the mark 75, so that the worker can start work of placing the frozen egg above 71 without adjusting or by slightly adjusting the focal distance of the microscope 100.　At this time, by lightly holding down the device body 72 of the freezing device 70 toward the inclined surface 61 of the groove part 60 by the left finger that has moved the dish body 40, it is possible to reliably fix the top 71.　Similarly to the conventional technique, compared to a case where the freezing device 70 is held by the left hand or the like, it is possible to reliably fix to the visual field of the microscope 100 the placement position of the frozen egg near the distal end of the top 71, and keep the microscope 100 in the state where the focus is also adjusted.　Furthermore, the top 71 inclines at the inclination angle θ (theta) of the inclined surface 61, so that, when the pipette is brought closer to place the frozen egg, the top 71 having the elasticity accepts the pipette, and it is also easy to place the frozen egg.　Note that, even when the position of the freezing device 70 in the groove part 60 is adjusted in advance, the focus does not necessarily completely match with the placement position of the frozen egg.　Furthermore, when a plurality of frozen eggs are sequentially pushed out from the pipette, and placed on the top 71, if the placement position moves, the focus is also out of focus.　In such a case, by moving the freezing device 70 along the groove part 60 by the finger tip that has held down the device body 72, it is possible to easily adjust the focus.　Moreover, the freezing device 70 moves only along the Y direction, so that the top 71 does not go out of the visual field of the microscope 100 as a result of the movement of the freezing device 70.

[0026] As described above, the rectangular dish 20 according to the present embodiment is a familiar four-well dish that includes the multiple wells 51 to 54, and makes it very easy to handle frozen eggs using the freezing device 70.　It is not necessary to grip the freezing device 70 by the hand and determine a three-dimensional position, and, once the distal end of the freezing device 70 or the like is brought into the visual field of the microscope 100, it is also possible to adjust the focus by lightly sliding the device body 72 of the freezing device 70.　That is, by only moving the dish body 40 and moving the freezing device 70 in the groove part 60 of the dish body 40, the worker can easily place the frozen egg at the top 71 of the freezing device 70 in the visual field of the microscope 100.　It is not necessary to temporarily put the freezing device 70 somewhere to adjust the focus of the microscope 100 or change the hand of the pipette, and it is possible to concentrate on placing the frozen eggs on the top 71.　As a result, it is possible to complete the work in a short time, and suppress an influence of the outside world on frozen eggs.

[0027] (A4) Function and Effect of Recess Part: In the present embodiment, the shape of the bottom part 57 of the recess part 55 formed between the wells 51 to 54 provided apart from each other is a shape that is shallow compared to conventional dishes and forms a slope toward the end parts as illustrated in Fig. 11.　Fig. 11 illustrates, in an upper part, the shape of the recess part 55 according to the present embodiment.　Furthermore, Fig. 11 illustrates, in a lower part, a state where a difference between the shapes of the recess part 55 according to the present embodiment and the conventional recess part is overlaid.　As illustrated in Fig. 11, in a recess part 55A of a conventional four-well dish, a bottom part BT is deeper than the recess part 55 according to the present embodiment, and a slope is not formed therein, either.　Hence, the volume of the entire recess part 55 becomes large.　To fill a cleaning solution or a culture solution to a predetermined depth of this recess part 55A, a considerable liquid amount is necessary, and a usage of each solution increases.

[0028] By contrast with this, in the recess part 55 according to the present embodiment, the bottom part is shallow and the slope is formed toward the end parts, so that small liquid amounts that are necessary to fill a cleaning solution or a culture solution to the same depth are sufficient.　Moreover, although the conventional recess part 55A does not have a slope and therefore has corner parts CH, there are not these corner parts CH in the present embodiment.　The corner parts CH make it difficult to find a frozen egg or the like under a microscope when the frozen egg is moved by a pipette or the like and reaches the vicinity of this CH.　Spending a time to find a frozen egg or the like lost in a well takes a longer time required for freezing as a whole accordingly.　It may not be preferable for frozen eggs or the like to be exposed to the outside world for a long time, and it is preferable for fertility treatment to avoid such a situation.

[0029] Although the above description has cited the example of work of embryo freezing for placing fertilized eggs or the like at the distal end of the freezing device 70 to freeze the fertilized eggs and the like, the work is not limited to such work, and work of embryo thawing of thawing frozen frozen eggs can be performed with the freezing device 70 put in the groove part 60 likewise, so that it is possible to improve workability.　Members to be placed in the groove part 60 are not limited to the freezing device 70, and may be any member as long as the member is the bar-shaped member to be used together with the rectangular dish 20.　Furthermore, the width of the groove part 60 or the like may be set according to the thickness of the bar-shaped member.

[0030] B.　Other Embodiment: (1) One of the other embodiments of the present disclosure is a dish including multiple wells.　This dish includes a groove part at a position on an outer side of the well of the dish, the groove part accommodating part of a bar-shaped member used for a process of handling a fertilized egg on the dish, the groove part has both ends that are open ends, the groove part has an inclined structure that accommodates the bar-shaped member in a state where the bar-shaped member is inclined with respect to a horizontal plane in a formation direction of the groove part, and a lower part height that is a height of the open end having a lower height from a dish bottom surface of a bottom part of the groove part among the open ends is higher than a height of a bottom part of one of the multiple wells.　Thus, it is possible to accommodate the bar-shaped member in this groove part, and handle the accommodated bar-shaped member together with the dish.　Furthermore, the bar-shaped member is accommodated in the state where the bar-shaped member is inclined with respect to the horizontal plane, so that it is possible to adjust the position in the height direction near the distal end of the bar-shaped member by moving the bar-shaped member in the formation direction of the groove part.

[0031] Such a dish may be rectangular or round.　Fig. 12 illustrates a configuration example of a round dish 20B.　In this dish 20B, a groove part 60B is formed near the outer circumference thereof.　What is the same as that in the above embodiment is that the groove part 60B includes the inclined surface that inclines at the predetermined angle θ (theta).　The width of the groove part may be determined in accordance with a member to be placed.　Note that a deformable member may be provided inside the groove part to support the thickness of the bar-shaped member.　Furthermore, the number of wells may be plural, and various types whose number of wells are two or more, three, five, or six can be implemented.　The number of wells in the illustrated dish 20B is three, and the dish 20B includes wells 51B, 52B, and 53B.　The shapes of the wells may be identical or different.　Furthermore, a recess part 55B is formed between these wells 51B and 53B provided apart from each other.　Similarly to the above embodiment, in a bottom part of the recess part 55B, the center part is the deepest, and a slope that becomes shallower toward end parts is formed.　Consequently, an end surface cut along illustrated line XI-A is substantially the same as the shape illustrated in the VII-A end view in the upper part in Fig. 11.

[0032] (2) According to the above configuration, an inclination angle at which the inclined structure accommodates the bar-shaped member in a state where the bar-shaped member inclines with respect to the horizontal plane may be four degrees or more and 10 degrees or less with respect to the horizontal plane.　By so doing, the angle with respect to the horizontal plane is provided, so that it is also possible to adjust the position in the vertical direction of the distal end of the bar-shaped member by moving the bar-shaped member along the formation direction of the groove, and moreover adjust a movement amount in the vertical direction in a range of approximately 7% to approximately 18% compared to a movement amount in the horizontal direction.　Furthermore, the bar-shaped member accepts at an angle of four degrees to 10 degrees an object such as a distal end of a pipette that approaches the bar-shaped member, so that it is easy to perform work using the bar-shaped member.　This angle is desirably four to six degrees.　This angle range makes it possible to provide an effect of enhancing workability of the worker when, for example, the bar-shaped member is the freezing device for freezing embryos.　The level of workability results from the answers “easy to use” collectively made by a plurality of workers in a sensory test.

[0033] (3) According to the above configuration, the inclined structure of the groove part may be a structure that the bottom part of the groove part is inclined with respect to the horizontal plane in the formation direction of the groove part.　By so doing, it is possible to easily implement the configuration that accommodates the bar-shaped member in an inclined state.　In this regard, the inclined surface does not necessarily need to be provided, and a configuration may be employed where, for example, the bar-shaped member is supported at two points apart in the formation direction of the groove part.

[0034] (4) According to the configuration in above (3), an angle of the bottom part of the groove part with respect to the horizontal plane may be four degrees or more and 10 degrees or less.　By so doing, the bar-shaped member can be easily accommodated at this angle, so that it is possible to obtain the above function and effect.

[0035] (5) According to the configuration in above (1) to (4), the lower part height is higher than the height of the bottom part of all of the multiple wells.　By so doing, even when any well is used, it is possible to adjust the height of the distal end of the bar-shaped member or the vicinity thereof to the height equal to that of the bottom part of the well.　Consequently, when the distal end of the bar-shaped member is put in the visual field of the microscope, it is possible to easily adjust to the distal end of the bar-shaped member the focus of the microscope in a state where the focus is adjusted to the bottom part of the well.　Note that the height of the bottom part of the well is lower than the lower part height of the groove part in some wells, and is higher than the lower part height of the groove part in some wells.

[0036] (6) According to the configuration in above (1) to (5), the dish may further include a lid body that covers the multiple wells, and the groove part may be provided at a position covered with the lid body on the dish to which the lid body has been attached.　By so doing, the groove part is covered with the lid body likewise in a state where the lid body covers the well, so that it is possible to keep the groove part clean similarly to the well.　Note that the groove part may be disposed outside the lid body.　In this case, a cover dedicated to the groove part may be also prepared.

[0037] (7) According to the configuration in above (1) to (6), the number of the multiple wells may be N (N represents an integer equal to or more than three), and the N wells may be disposed spaced apart from each other, a recess part that is a single recess including a center part surrounded by the N wells, and a narrow part sandwiched by two adjacent wells may be provided between the N wells, the single recess part may be the deepest at the center part, and the single recess part may include a slope that becomes shallower from the center part to an end part of the narrow part.　By so doing, it is possible to reduce the volume of the recess part provided between the wells.　Consequently, it is possible to reduce a usage of a liquid such as a reagent or a cleaning liquid for work or a culture solution that is injected into this recess part to use.　This is because, although a predetermined depth (water depth) of the liquid used for work is required, the water depth of the entire recess part may not be the predetermined depth, and it is sufficient that part of the water depth satisfies the predetermined depth.　This dish includes the slope whose center part is deep and that becomes shallower toward the end parts, so that it is also easy to perform work that needs to make a distal end of a pipette reach a deep part of the water depth.　Furthermore, although the water depth having the depth corresponding to the end of the recess part makes it difficult to find fertilized eggs, embryos, cell debris, or the like that have entered the end of the recess part, the slope that becomes shallower toward the end parts hardly causes this difficulty.

[0038] (8) According to the configuration in above (1) to (7), the dish may be a rectangular dish that has at least four faces on an outer circumference, the number of the multiple wells may be four or six, and the four wells may be disposed at four corners of a square or a rectangle, a recess part that is a recess of a cross shape may be provided between the four wells, the recess part of the cross shape may is the deepest at a center part, and the recess of the cross shape around the center part may include a slope that becomes shallower toward an end part of the cross shape.　By so doing, it is possible to dispose the wells that surround the recess part and the recess part on the dish.

[0039] (9) Another embodiment of the other embodiments is an embodiment of a rectangular dish including multiple wells.　According to this rectangular dish, the number of the multiple wells may be four or six, and the four wells may be disposed at four corners of a square, a recess part that is a recess of a cross shape may be provided between the four wells, the recess part of the cross shape may be the lowest and the deepest at a center part, and a recess part extending in a cross shape around the center part may include a slope that becomes shallower toward an end part.　By so doing, it is possible to reduce the volume of the recess part of the cross shape provided between the wells.　Consequently, it is possible to reduce a usage of a liquid such as a reagent or a cleaning liquid for work or a culture solution that is injected into this recess part to use.　Although a predetermined depth (water depth) of the liquid used for work is required, the water depth of the entire recess part of the cross shape may not be the predetermined depth, and it is sufficient that part of the water depth satisfies the predetermined depth.　This rectangular dish includes the slope whose center part is deep and that becomes shallower toward the end parts, so that it is also easy to perform work that needs to make a distal end of a pipette reach a deep part of the water depth.　Furthermore, although the water depth having the depth corresponding to the end of the recess part makes it difficult to find fertilized eggs, embryos, cell debris, or the like that have entered the end of the recess part, the slope that becomes shallower toward the end parts hardly causes this difficulty.

[0040] The present disclosure is not limited to the above-described embodiments, and can be implemented by various configurations without departing from the gist of the present disclosure.　For example, technical features in the embodiment corresponding to technical features in each aspect described in the summary can be replaced or combined as appropriate to solve part or all of the above-described problems or achieve part or all of the above-described effects.　Furthermore, the technical features that are not described as indispensable in this description can be deleted as appropriate.　For example, part of the components implemented by hardware in the above embodiments can be implemented by software.

[0041] The dish according to the present disclosure is used for a freezing treatment of fertilized eggs or embryos and, in addition, a thawing treatment of cryopreserved fertilized eggs or embryos, a treatment of collecting eggs from an antral fluid at a time of sampling, and the like.　Furthermore, such a dish may also be used for cryopreservation of sperms or the like.　The dish according to the present disclosure can be widely used in a field of a medical industry.

Claims

1. A dish comprising: multiple wells; and a groove part at a position on an outer side of the well of the dish, the groove part accommodating part of a bar-shaped member used for a process of handling a fertilized egg on the dish, wherein the groove part has both ends that are open ends, the groove part has an inclined structure that accommodates the bar-shaped member in a state where the bar-shaped member is inclined with respect to a horizontal plane in a formation direction of the groove part, and a lower part height that is a height of the open end having a lower height from a dish bottom surface of a bottom part of the groove part among the open ends is higher than a height of a bottom part of one of the multiple wells.

2. The dish according to claim 1, wherein the inclined structure which accommodates the bar-shaped member has an inclined angle, the inclined angel at which the bar-shaped member inclines with respect to the horizontal plane is four degrees or more and 10 degrees or less with respect to the horizontal plane.

3. The dish according to claim 1, wherein the inclined structure of the groove part is a structure that the bottom part of the groove part is inclined with respect to the horizontal plane in the formation direction of the groove part.

4. The dish according to claim 3, wherein the inclined structure of the groove part has an angle at which the bottom part of the groove part with respect to the horizontal plane is four degrees or more and 10 degrees or less.

5. The dish according to claim 1, wherein the lower part height is higher than the height of the bottom part of all of the multiple wells.

6. The dish according to claim 1, further comprising a lid body that covers the multiple wells, wherein the groove part is provided at a position covered with the lid body on the dish to which the lid body has been attached.

7. The dish according to any one of claims 1 to 6, further comprising a recess part, Wherein the number of the multiple wells is N (N represents an integer equal to or more than three), and the N wells are disposed spaced apart from each other; the recess part that is a single recess including a center part surrounded by the N wells, and a narrow part sandwiched by two adjacent wells is provided between the N wells; the single recess part is the deepest at the center part; and the single recess part includes a slope that becomes shallower from the center part to an end part of the narrow part.

8. The dish according to any one of claims 1 to 6, wherein the dish is a rectangular dish that has at least four faces on an outer circumference; the number of the multiple wells is four or six, and the four wells are disposed at four corners of a square or a rectangle; a recess part that is a recess of a cross shape is provided between the four wells, the recess part of the cross shape is the deepest at a center part; and the recess part of the cross shape around the center part includes a slope that becomes shallower toward an end part of the cross shape.

9. A rectangular dish comprising: four or six wells, four wells disposed at four corners of a square of the dish; and a recess part that is a recess of a cross shape is provided between the four wells, and wherein the recess part of the cross shape is the deepest at a center part, and a recess part extending in a cross shape around the center part becomes shallower toward an end part.

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