Sequential coding methods and related kits

ES3073780T3Undetermined Publication Date: 2026-07-15
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Patent Information

Authority / Receiving Office
ES · ES
Patent Type
Patents
Filing Date
2021-08-16
Publication Date
2026-07-15

AI Technical Summary

Technical Problem

Highly-parallel characterization and recognition of macromolecules, such as proteins, remains a challenge due to limitations in existing assays like immunoassays and mass spectrometry, which face issues with sensitivity, dynamic range, cross-reactivity, and background signals, making high-throughput macromolecule analysis inefficient and inaccurate.

Method used

A method involving nucleic acid encoding and barcoding of molecular recognition events, utilizing a recording tag joined to a support, binding agents with coding tags, and enzymatic reactions like ligation, extension, and cleavage to transfer identifying information from coding tags to recording tags, enabling efficient and high-throughput macromolecule analysis.

Benefits of technology

The method achieves high-throughput, accurate, and sensitive analysis of macromolecules by effectively transferring identifying information, reducing the need for spacers and minimizing DNA-DNA interactions, thereby enhancing the efficiency and accuracy of protein sequencing and characterization.

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Abstract

This disclosure relates to methods and kits for the analysis of macromolecules. In some embodiments, it relates to macromolecule analysis methods that employ barcoding and nucleic acid coding to identify molecular recognition events. A method and related kits are also provided for information transfer using various enzymes, including ligation, extension, and cleavage reactions with nucleic acid molecules associated with the macromolecule of analysis. In some embodiments, the macromolecule of analysis comprises a peptide, a polypeptide, or a protein.
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