Composition for the nutrition or drink of a non-human animal

Abstract

The present invention relates to a composition for the nutrition or beverage of a non-human animal comprising a combination of spirulina and Ascophyllum nodosum. It also relates to the use of this composition to improve the veterinary performance of non-human animals, in order to reduce greenhouse gas emissions, particularly methane, in a non-human mammal, to increase the digestibility of dry matter and fiber, particularly forage and concentrates, and / or to improve the intake rate and / or diet efficiency and / or to promote weight gain and / or to increase the intensity of fermentation and microbial metabolism and / or to inhibit protozoan flora and / or to reduce protein degradation and / or to direct fermentation towards the production of volatile fatty acids in a non-human mammal, in order to improve meat quality and / or growth performance and / or the welfare of a non-human animal.It also relates to the composition according to the invention, for use in improving intestinal health and / or intestinal well-being and / or the immune system and / or bone mineralization and / or bone structure quality and / or as an antibacterial agent in non-human animals and / or to increase intestinal villi in non-human monogastric animals and / or to modulate the inflammatory response and / or to prevent damage associated with post-weaning nutritional stress in monogastric mammals.

Description

Technical Field

[0001] The present invention relates to the field of non-human animal food and / or drink, and in particular to the combination of extracts of spirulina algae and Ascophyllum nodosum for the purpose of non-human animal food and / or drink for livestock, more particularly livestock. Previous technique

[0002] Application WO 2004 / 080196 describes the use of products derived from macroalgae, microalgae, plants, and / or fungi in animal feed, as a replacement for animal products, particularly fish, due to their high content of long-chain polyunsaturated fatty acids (LC-PUFAs) such as DHA (docosahexaenoic acid) and EPA (eicosapentaenoic acid). Spirulina is cited as a microalga, and Ascophyllum as a macroalga. However, these algae are listed among a long list of other possible microalgae or macroalgae. Furthermore, no specific example includes a mixture of these two algae. Moreover, these algae are not used in their raw form or as extracts, but rather as derived products.

[0003] Application WO 2001 / 22822 describes the use of a composition comprising a cytokine derived from 'Ascophyllum nodosum mixed with mineral-based nutrients, a vitamin B mixture, and acetylsalicylic acid or one of its salts, in the form of a drink or injectable solution, to improve the animals' immunity. The cytokine resulting d'Ascophyllum nodosum is obtained by extraction using a very specific process. The application describes the possible presence in the composition of other ingredients such as spirulina, which is cited in a long list of ingredients, without any example of the combination of these two algae ( Ascophyllum nodosum and spirulina) should not be disclosed.

[0004] Patent application EP1570845 describes the use of acid polysaccharides as an agent to enhance the production of bone morphogenetic protein or improve osteogenesis. The acid polysaccharide can be derived from algae such as spirulina, among others, or l'Ascophyllum nodosum. However, this document does not disclose any specific examples containing acidic polysaccharides, particularly sulfated ones, from both Ascophyllum nodosum and spirulina.

[0005] Application KR2020-0129841 describes in its examples (

[0051] -

[0059] ) an animal model (rat) to which an aqueous extract of spirulina (SP at 800 mg / kg / day) is administered orally or d'Ascophilum Nodosum (AN at 100mg / kg / day or 200mg / kg / day) mixed with water. However, aqueous extracts of spirulina and d ' Ascophilum nodosum are used separately. Furthermore, their use is not for improving the welfare of an animal but to reduce or eliminate the toxicity of lead ingested by animals, the purpose of these tests being to obtain a drug for human use.

[0006] Furthermore, although this request describes aqueous extracts of spirulina or d'Ascophilum Nodosum (

[0032] ) used in animal drink, it does not describe a protein extract of spirulina (and even less a protein hydrolysate) nor a polysaccharide extract of Ascophilum nodosum.

[0007] Finally, rats are not farm animals, and even less so livestock.

[0008] Patent application DE 19608563 describes food compositions comprising a combination of raw spirulina and d'Ascophilum nodosum raw and lithothamnium.

[0009] However, nowhere in this application is it stated that these compositions are intended for feeding animals, in particular livestock and even less so farm animals.

[0010] These compositions are actually intended for the humans on whom they have been tested (examples).

[0011] Application ITPD20-110179 describes a composition for controlling dysglycemia in an animal.

[0012] However, this is not a farm animal and even less a livestock animal (such as poultry, ruminants, pigs and / or aquatic animals) but a domestic animal of the cat or dog type.

[0013] Indeed, farm animals do not suffer from dysglycemia. It is a disease typical of companion animals. The composition described in this application is therefore not suitable for farm animals or livestock.

[0014] Furthermore, the composition described in this application must contain an extract of Gymnema Sylvestre, an excerpt from Momordica Charantia and zinc, which is not the case in the composition according to the invention.

[0015] Furthermore, this application does not describe any spirulina extract or d'Ascophilum Nodosum even less so a spirulina protein extract (and even less so a protein hydrolysate) or a polysaccharide extract d'Ascophilum nodosum.

[0016] However, the inventors surprisingly discovered that the combination of polysaccharide extract d'Ascophyllum nodosum and spirulina protein extract had a synergistic action to improve the zootechnical performance of non-human farm animals, particularly livestock, advantageously of a ruminant and / or a monogastric such as poultry or a pig and / or an aquatic animal such as a fish or a crustacean, when the combination was administered in a food composition or a drink. Description of the invention

[0017] The present invention therefore relates to a composition for the nutrition or drinking of a non-human farm animal, more particularly a livestock animal, advantageously a ruminant and / or a monogastric animal such as poultry or a pig and / or an aquatic animal such as a fish or a crustacean, comprising the combination of a protein hydrolysate of spirulina and a polysaccharide extract. d'Ascophyllum nodosum.

[0018] The composition according to the invention therefore includes spirulina.

[0019] Spirulina is a microalga (35µm wide and 200µm long on average) recognizable by its distinctive spiral shape. Its scientific name is Arthrospira. It is a cyanobacterium belonging to the class of Cyanophyceae (blue-green algae). Its color is due to the presence of phycocyanin (a protein), a blue pigment used particularly in the food industry. There are nearly 1,500 species of blue-green algae, and 36 species of spirulina are edible. The main species currently available on the market is... Spirulina platensis. Spirulina according to the invention is therefore advantageously the Spirulina platensis. Spirulina contains proteins (including phycocyanin), various carotenoids (mainly beta-carotene, but also cryptoxanthin, lutein, zeaxanthin, etc.), vitamins and minerals including iron, magnesium, calcium, vitamins A, B, E. Spirulina also contains a good amount of essential amino acids (such as aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine and arginine) and gamma-linolenic acid, an unsaturated fatty acid of the omega-6 family. In particular, its protein content is between 55 and 70% (inclusive), advantageously greater than or equal to 60%, particularly between 60 and 70% (inclusive), more advantageously between 65 and 69% (inclusive), by weight relative to the total weight of the spirulina.

[0020] Furthermore, spirulina is rich in phycocyanin, the only natural blue pigment that can be used as a food coloring and to which significant antioxidant activity is attributed, particularly when its content is greater than or equal to 7%, more advantageously greater than or equal to 8%. It also contains chlorophyll.

[0021] The spirulina according to the invention can therefore be used in powder form. Advantageously, it is not used in hydrogel form. Even more advantageously, it does not contain mucilage. In a particularly advantageous embodiment, the spirulina according to the invention is not used in combination with, or in a composition containing, an extract of Gymnema Sylvestre and / or an extract from Momordica Charantia and / or zinc.

[0022] Spirulina is found in the form of a protein hydrolysate.

[0023] In particular, the extraction is carried out by maceration, advantageously in an aqueous solvent, more advantageously water, more particularly at a concentration of 100g of dehydrated spirulina in 900g of solvent, particularly at a temperature between 30 and 80°C (inclusive), more advantageously between 50 and 70°C (inclusive), particularly 60°C, for a duration advantageously between 1 and 6 hours (inclusive), more advantageously between 2 and 4 hours (inclusive). Advantageously, the protein hydrolysate is obtained by hydrolysis of the protein extract, particularly by enzymatic hydrolysis, more advantageously using an endoprotease enzyme such as serine endoproteases like subtilisin, advantageously marketed by Novozymes under the trade name Novo-Pro®< D.Hydrolysis advantageously takes place in aqueous solution, particularly at a pH between 7 and 11 (inclusive), more particularly between 7 and 10 (inclusive), even more particularly between 8 and 9.5 (inclusive), particularly from 8, advantageously at a temperature between 55 and 75°C (inclusive), more advantageously between 55 and 65°C (inclusive), particularly from 60°C.

[0024] In an advantageous embodiment, the extract is in liquid form.

[0025] The protein hydrolysate advantageously contains polypeptides, peptides, and amino acids (such as aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine, and arginine). Advantageously, the molecular weight of the polypeptides and peptides can range from less than 150 Da to more than 20,000 Da. Advantageously, the protein hydrolysate comprises more than 85% by weight of polypeptides, peptides, and amino acids having a molecular weight less than or equal to 5,000 Da, advantageously more than 90% by weight, and in particular more than 92% by weight relative to the total weight of polypeptides, peptides, and amino acids in the protein hydrolysate.Advantageously, the protein hydrolysate comprises more than 70% by weight of polypeptides, peptides, and amino acids having a molecular weight less than or equal to 1000 Da, advantageously more than 75% by weight, in particular more than 80% by weight, relative to the total weight of polypeptides, peptides, and amino acids in the protein hydrolysate. Advantageously, the protein hydrolysate comprises more than 50% by weight of polypeptides, peptides, and amino acids having a molecular weight less than or equal to 500 Da, advantageously more than 60% by weight, in particular more than 65% by weight, relative to the total weight of polypeptides, peptides, and amino acids in the protein hydrolysate.

[0026] Advantageously, the composition according to the invention comprises between 0.01 and 0.5% (inclusive) by weight relative to the total weight of the composition, advantageously between 0.03 and 0.4% (inclusive) by weight relative to the total weight of the composition, and even more advantageously between 0.02 and 0.2% (inclusive) by weight relative to the total weight of the composition, of spirulina protein hydrolysate. The spirulina content of the composition depends in particular on the animal for which the feed or beverage is intended.

[0027] Thus, in particular, the composition according to the invention comprises 0.2% by weight, relative to the total weight of the composition, of spirulina protein hydrolysate, when the composition is intended for swine, more advantageously when it is intended for pigs or piglets, weaned or not, particularly when it is intended for piglets, weaned or not. More particularly, the composition according to the invention comprises between 0.02% and 0.1% (inclusive) by weight, relative to the total weight of the composition, of spirulina protein hydrolysate, when the composition is intended for birds, particularly farm birds, more particularly poultry, more advantageously when it is intended for gallinaceous birds such as chickens or chicks.

[0028] Advantageously, the protein hydrolysate content of spirulina, administered to ruminants, in particular to dairy cows, using the composition according to the invention, is between 2 and 70g / day (inclusive limits), in particular between 5 and 50g / day (inclusive limits), more advantageously it is 5g / day, for a ration of about 15kg of forage per day.

[0029] The composition according to the invention further comprises l'Ascophyllum nodosum.

[0030] L'Ascophyllum nodosum, also called black seaweed, knotty seaweed, is a macroalga (it can reach over 1.50 m in length) of brown color that belongs to the class of Phaeophyceae. She is the only currently recognized representative of the genre Ascophyllum, in the family of Fucaceae.

[0031] It contains high levels of macro-elements (such as N, P, K, Ca, Mg, S) and trace elements (e.g., Mn, Cu, Fe, Zn, etc.). It also contains phytohormones such as betaine, cytokinin, auxin and gibberellin (growth stimulants), mannitol, organic acids, polysaccharides, amino acids and proteins.

[0032] L 'Ascophyllum nodosum According to the invention, it can therefore be used in powder form.

[0033] In an advantageous way l'Ascophyllum nodosum according to the invention is not used in hydrogel form. Even more advantageously, it does not contain AOS (alginatooligosaccharides). In a particularly advantageous embodiment, the 'Ascophyllum nodosum according to the invention is not used in association with or in a composition containing an extract of Gymnema Sylvestre and / or an extract from Momordica Charantia and / or zinc.

[0034] However, the 'Ascophyllum nodosum is found in the form of a polysaccharide extract (in particular water-soluble), advantageously the extract does not contain cytokine, more advantageously the extract has been clarified, even more advantageously dehydrated.

[0035] In particular, the extraction is carried out by maceration, advantageously in an aqueous solvent, more advantageously water, particularly at a concentration of 200g of 'Ascophyllum nodosum dehydrated in 800g of solvent, in particular at a temperature between 30 and 95°C (inclusive), more advantageously between 45 and 90°C (inclusive), for a duration advantageously between 1 hour and 6 hours (inclusive), more advantageously between 2 hours and 4 hours (inclusive).

[0036] In an advantageous embodiment, the extract is in liquid form. In an even more advantageous embodiment, it is in powder form.

[0037] Advantageously, the composition according to the invention comprises between 0.05 and 0.5% (inclusive) by weight relative to the total weight of the composition, advantageously between 0.06 and 0.15% (inclusive) by weight relative to the total weight of the composition, more advantageously between 0.01 and 0.1% (inclusive) by weight relative to the total weight of the composition, of a polysaccharide extract of Ascophyllum nodosum. The content in Ascophyllum nodosum The composition depends in particular on the animal for which the food or drink is intended.

[0038] In particular, the composition according to the invention comprises between 0.02% and 0.1% (inclusive) by weight relative to the total weight of the composition, of polysaccharide extract d'Ascophyllum nodosum, when the composition is intended for pigs, more advantageously when it is intended for pigs or piglets, weaned or not, in particular when it is intended for piglets, weaned or not.

[0039] More particularly, the composition according to the invention comprises between 0.01% and 0.05% (inclusive) by weight relative to the total weight of the composition, of polysaccharide extract d'Ascophyllum nodosum, when the composition is intended for birds, especially farm birds, more particularly for poultry, more advantageously when it is intended for gallinaceous birds such as chickens or chicks.

[0040] Advantageously, the polysaccharide extract content of Ascophyllum nodosum, administered to ruminants, in particular to dairy cows, using the composition according to the invention, is between 5 and 50g / day (inclusive limits), in particular between 10 and 20g / day (inclusive limits), more advantageously it is 10g / day, for a ration of about 15kg of forage per day.

[0041] Advantageously, the ratio by weight of spirulina protein hydrolysate / polysaccharide extract d'Ascophyllum nodosum is within the range of 0.02-100 (inclusive), advantageously 0.2-15 (inclusive), particularly 0.5-20 (inclusive), and more specifically between 1 / 2 and 3 / 1 (inclusive). This ratio depends in particular on the animal for which the feed or drink is intended.

[0042] In particular, this ratio is within the range of 2-10 (inclusive), when the composition is intended for swine, more advantageously when it is intended for pigs or piglets, weaned or not, in particular when it is intended for piglets, weaned or not.

[0043] More specifically, this ratio falls within the range of 2-20 (inclusive) when the composition is intended for birds, particularly farm birds, and more specifically for poultry, and more advantageously when intended for gallinaceous birds such as chickens or chicks. Advantageously, this ratio falls within the range of 0.04-14 (inclusive), advantageously 0.25-5 (inclusive), more advantageously 0.5-2.5 (inclusive), and even more advantageously 0.5, when the composition is intended for ruminants, particularly dairy cows, and more specifically for animals receiving a daily ration of 15 kg of forage.

[0044] The composition according to the invention is therefore intended for oral administration to animals. It may be a food composition or a beverage, advantageously a food composition.

[0045] The composition according to the invention may further comprise lithothamnium. It may also comprise other ingredients known to those skilled in the art for animal nutrition or drinking, such as, for example, fodder in the case of ruminants or water in the case of drinks and / or cobs in the case of poultry.

[0046] In an advantageous embodiment, the composition according to the invention does not contain any extract of Gymnema Sylvestre and / or extract from Momordica Charantia and / or zinc.

[0047] The composition according to the invention can have any form suitable for feeding or drinking non-human animals, in particular a solid form, a powder or granule form, or a liquid or gel form.

[0048] It can therefore be added directly to the ration of non-human animals, or added to premixes or food supplements or to nutritional blocks or buckets.

[0049] In an advantageous embodiment, it is added to the daily ration of a non-human animal, in particular a ruminant such as, for example, a dairy cow, advantageously at a rate of 5 mg / day of composition per 1 kg of dry matter of ration.

[0050] This ration for ruminants can, for example, consist of forages of all types and in all their forms (green, dehydrated, ensiled, pelleted, etc.) such as grass and other forage grasses, forage cereals (barley, corn, oats, wheat, sorghum, soybeans, rye), legumes (peas, broad beans, lupins, soybeans, alfalfa, sainfoin, clovers), roots, tubers and their by-products (beets, beet pulp, potatoes, potato pulp, etc.), cabbage, rapeseed, sunflowers, plant waste (haulms, cobs, cereal husks, bran, shelled corn cobs, bagasse) and starches, by-products of the agri-food industry (starch production, ethanol production, brewing, milling, etc.), as well as seed cakes oilseeds, syrups, and nitrogenous food materials such as urea and its derivatives (biuret, ureides) and ammonium salts.

[0051] Preferably, the appropriate ration for ruminants according to the invention comprises forages, preferably maize silage typically representing 10 to 50% of the dry matter ingested, preferably daily, preferably combined with other forages such as hay, straw or grass or cereal silage and preferably supplemented with concentrated feeds such as cereals, oilseed cakes or compound feeds.

[0052] In particular, it is a mixture of corn silage, hay and concentrates (consisting for example of wheat, corn, barley, beet pulp, wheat bran and molasses), for example in a ratio of 50:30:20, or of hay, concentrate and corn starch, for example in a ratio of 77:9:12:2.

[0053] The composition is intended for a non-human farm animal, more specifically a livestock animal, advantageously a ruminant and / or a monogastric animal and / or an aquatic animal. In particular, it may be a mammal.

[0054] For the purposes of this invention, "livestock" or "production animal" means any animal raised or kept for its profitability, that is to say, "the production of foodstuffs, wool, hides or other agricultural purposes".

[0055] Among animals, herbivores and in particular ruminants are mammals that have a diet based on plant fibers containing, among the soluble fibers, insoluble fibers of the cellulose and hemicellulose type, fibers that are very poorly digestible.

[0056] Ruminants have the unique characteristic of digesting food through a "fermentation chamber," the rumen (130-180 liters), located in the anterior part of the digestive tract. The rumen contains a wide variety of microflora, including bacteria and protozoa, which perform a highly efficient and primarily fermentative predigestion. This microbial flora thus determines the digestibility of carbohydrates and proteins, as well as the body's ability to obtain vitamins and other nutrients.

[0057] In an advantageous embodiment, the composition according to the present invention is intended for the nutrition and / or drinking of non-human farm mammals, more particularly livestock, in particular herbivores (such as ruminants, horses, lagomorphs) and more particularly ruminants.

[0058] Ruminants include, for example, cattle, sheep, goats, deer and camelids.

[0059] For the purposes of this invention, "bovine" refers to a subfamily of bovines comprising several important species of livestock. Bovines include, in particular, the cow, especially the dairy cow, the suckler cow, the heifer, the calf, the weaned calf, the young bull, the ox, the fattening ox, the bull, the buffalo, the yak, the gayal, and the banteng.

[0060] For the purposes of this invention, "ovine" means ruminant herbivores of the genus Ovis. Ovins include in particular mouflon, sheep, ewes, grazing sheep and lambs.

[0061] For the purposes of this invention, "goat" refers to ruminant herbivores of the genus Capra. Goats include, in particular, the goat, the ram, the kid, and the ibex.

[0062] For the purposes of this invention, "cervid" means ruminants of the Cervidae family, bearing antlers. Cervids include, in particular, the red deer, the stag, the yearling, the doe, the fawn, the roe deer, the buck, the doe, the reindeer, the fallow deer, the fallow deer, and the elk.

[0063] For the purposes of this invention, "camelid" refers to artiodactyl mammals of the Camelidae family. Camelids include, in particular, the dromedary, the camel, the female camel, the llama, and the alpaca. In an advantageous embodiment, the ruminant according to the invention is a bovine, specifically chosen from among the cow, particularly the dairy cow, the calf, the weaned calf, the suckler calf, the steer, and the fattening steer, and more particularly, preferably, the dairy cow.

[0064] In an advantageous embodiment, the composition according to the present invention is intended for the nutrition and / or drinking of an aquatic animal.

[0065] Aquatic animals can include fish, especially freshwater, brackish or saltwater, farmed, especially commercial, such as salmon, trout, bass, carp, tilapia, crucian carp, catfish, roach, sea bream, meagre, turbot, sturgeon and pangasius.

[0066] Aquatic animals can also include crustaceans such as shrimp or crayfish.

[0067] In an advantageous embodiment, the composition according to the present invention is intended for the nutrition and / or drinking of non-human farm mammals, more particularly livestock, in particular monogastrics, and more particularly omnivores (pigs).

[0068] For the purposes of this invention, "pig" means mammals of the Suidae family of the genus Sus. Pigs include in particular the meat pig (or fattening pig), the weaned or unweaned piglet, the sow, the boar and the gilt.

[0069] In an advantageous embodiment, the composition according to the present invention is intended for the nutrition and / or drinking of farm birds, and even more particularly of livestock and in particular poultry.

[0070] For the purposes of this invention, "poultry" means domestic birds kept by a human mammal. Poultry may belong to the order Galliformes or to the order Palmipedes.

[0071] For the purposes of this invention, "gallinaceous" or "waterfowl" refers to animals whose meat or egg products are intended for human consumption. Gallinaceous birds include, in particular, laying hens, quail, roosters, turkeys, guinea fowl, pigeons, pheasants, and especially broiler chickens (including chicks). Waterfowl include, in particular, ducks and geese.

[0072] The present invention further relates to the use of the composition according to the invention to improve the zootechnical performance of a ruminant and / or an omnivore, for example a pig.

[0073] It also relates to the use of the composition according to the invention to reduce the emission of greenhouse gases, in particular methane, in a ruminant.

[0074] Finally, it relates to a composition according to the invention, for its use in improving the immune system in non-human farm animals, advantageously ruminants and / or monogastrics such as birds, for example poultry, or omnivores, for example pigs, and / or aquatic animals such as fish or crustaceans, in particular in chickens, chicks, pigs, or piglets.

[0075] The present invention will be better understood in the light of the description in the figures and examples that follow, which are given by way of illustration. Brief description of the drawings

[0076] There figure 1 represents the concentration in % and the volume in ml / g of dry matter (DM) of CH4 after 24 hours of fermentation in vitro for a winter ration for the control, the protein hydrolysate of Spirulina platensis(E1) at 5g / day or 50g / day, the polysaccharide extract d'Ascophyllum nodosum (E2) at 10g / day or 20g / day or the combination of protein hydrolysate of Spirulina platensis and polysaccharide extract d'Ascophyllum nodosum (E1+E2 at 50g / day + 20g / day or 5g / day + 10g / day) under the conditions of example 2. The figure 2 represents the digestibility of dry matter (dDM), ADF (dADF) and NDF (dNDF) as a percentage after 24 hours of fermentation in vitro for a winter ration for the control - and the composition according to the invention, the protein hydrolysate of Spirulina platensis (E1) at 5g / day or 50g / day, the polysaccharide extract d'Ascophyllum nodosum (E2) at 10g / day or 20g / day or the combination of protein hydrolysate of Spirulina platensis and polysaccharide extract d'Ascophyllum nodosum (E1+E2 at 50g / day + 20g / day or 5g / day + 10g / day) under the conditions of example 2. The figure 3 represents the concentration in % and the volume in ml / g of dry matter (DM) of CH4 after 24 hours of fermentation in vitrofor a winter ration with an acidogenic feed for the control, lithothamnium (CalseaPowder Advance CPA) at 80g / day, protein hydrolysate of Spirulina platensis (E1) at 5g / day, the polysaccharide extract of Ascophyllum nodosum (E2) at 10g / day, the combination (simple mixture) of lithothamnium and protein hydrolysate of Spirulina platensis (CPA + E1 at 80g / day + 5g / day), the combination (simple mixture) of lithothamnium and polysaccharide extract of Ascophyllum nodosum (CPA + E2 at 80g / day + 10g / day) or the combination (simple mixture) of lithothamnium, protein hydrolysate of Spirulina platensis and of polysaccharide extract of Ascophyllum nodosum (CPA+E1+E2 at 80g / day + 5g / day + 10g / day) under the conditions of example 2. The figure 4 represents the concentration in % and the volume in ml / g of dry matter (DM) of CH4 after 24 hours of fermentation in vitro for a winter ration with an acidogenic feed for the control, lithothamnium (CalseaPowder Advance CPA) at 80g / day, protein hydrolysate of Spirulina platensis (E1) at 5g / day, the polysaccharide extract of Ascophyllum nodosum(E2) at 10g / day, the combination (impregnation of the extract onto the lithothamnium) of lithothamnium and protein hydrolysate of Spirulina platensis (CPA + E1 at 80g / day + 5g / day), the combination (impregnation of the extract onto the lithothamnium) of lithothamnium and polysaccharide extract of Ascophyllum nodosum (CPA + E2 at 80g / day + 10g / day) or the combination (impregnation of extracts onto lithothamnium) of lithothamnium, protein hydrolysate of Spirulina platensis and of polysaccharide extract of Ascophyllum nodosum (CPA+E1+E2 at 80g / day + 5g / day + 10g / day) under the conditions of example 2. The figure 5 represents the Beta-globulin content in the blood (%) of piglets at day 70 that received the control feed (Control T1), the Spirulina platensis crude at 0.2% (0.2% Spir. Crude T2), the association Spirulina platensis raw and of Ascophyllum nodosumcrude at 0.2% and 0.02% respectively (0.2% Spir. Crude + 0.02% Asco Crude T3), at 0.2% and 0.05% respectively (0.2% Spir. Crude + 0.05% Asco Crude T4), or at 0.2% and 0.1% respectively (0.2% Spir. Crude + 0.1% Asco Crude T5) under the conditions of Example 3 (not part of the invention). The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. The figure 6 represents the quantity of total blood protein in g / l of blood of piglets at day 70 that received the control feed (Control T1), the Spirulina platensis crude at 0.2% (0.2% Spir. Crude T2), the association Spirulina platensis raw and of Ascophyllum nodosumcrude at 0.2% and 0.02% respectively (0.2% Spir. Crude + 0.02% Asco Crude T3), at 0.2% and 0.05% respectively (0.2% Spir. Crude + 0.05% Asco Crude T4), or at 0.2% and 0.1% respectively (0.2% Spir. Crude + 0.1% Asco Crude T5) under the conditions of Example 3 (not part of the invention). The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. The figure 7 represents the length of the jejunal villi in micrometers of piglets at day 70 that received the control feed (Control T1), the Spirulina platensis crude at 0.2% (0.2% Spir. Crude T2), the association Spirulina platensis raw and of Ascophyllum nodosumcrude at 0.2% and 0.02% respectively (0.2% Spir. Crude + 0.02% Asco Crude T3), at 0.2% and 0.05% respectively (0.2% Spir. Crude + 0.05% Asco Crude T4), or at 0.2% and 0.1% respectively (0.2% Spir. Crude + 0.1% Asco Crude T5) under the conditions of Example 3 (not part of the invention). The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. The figure 8 represents the average daily consumption during the first phase (ages 28 to 42) in kg / piglet / day of animals receiving the control feed (Control T1), the Spirulina platensis crude at 0.2% (0.2% Spir. Crude T2), the association Spirulina platensis raw and of Ascophyllum nodosumcrude at 0.2% and 0.02% respectively (0.2% Spir. Crude + 0.02% Asco Crude T3), at 0.2% and 0.05% respectively (0.2% Spir. Crude + 0.05% Asco Crude T4), or at 0.2% and 0.1% respectively (0.2% Spir. Crude + 0.1% Asco Crude T5) under the conditions of Example 3 (not part of the invention). The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. The figure 9 represents the average daily gain (ADG) during the first phase (age between day 28 and day 710) in kg / piglet / day for animals receiving the control feed (Control T1), the Spirulina platensis crude at 0.2% (0.2% Spir. Crude T2), the association Spirulina platensis raw and of Ascophyllum nodosumcrude at 0.2% and 0.02% respectively (0.2% Spir. Crude + 0.02% Asco Crude T3), at 0.2% and 0.05% respectively (0.2% Spir. Crude + 0.05% Asco Crude T4), or at 0.2% and 0.1% respectively (0.2% Spir. Crude + 0.1% Asco Crude T5) under the conditions of Example 3 (not part of the invention). The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. The figure 10 represents the length of duodenal villi and depth of duodenal crypts in micrometers of 7-day-old chicks fed normal drinking water (control: water) or water mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum (E2) at respectively 0.1% of E1 + 0.02% of E2, 0.05% of E1 + 0.02% of E2 or 0.02% of E1 + 0.02% of E2 under the conditions of Example 4. The figure 11represents the depth of the duodenal crypts in micrometers measured in 7-day-old chicks fed a normal diet (control: cob + water - T1) or a diet containing cobs mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum (E2) at respectively 200 g / T of feed E1 + 100 g / T of feed E2 (T2), 600 g / T of E1 + 100 g / T of E2 (T3), 1000 g / T of E1 + 100 g / T of E2 (T4), 600 g / T of E1 + 500 g / T of E2 (T5) or 600 g / T of E1 + 500 g / T of E2 + Flavoring (T6) under the conditions of Example 4. The percentage values ​​indicate the difference in improvement compared to the control. The figure 12 represents the average daily gain (ADG) over the phase between days 27 and 35 in g / day in broiler chickens that received for 35 days a normal diet (control: cob + water - T1) or a diet containing cobs mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum(E2) at respectively 200 g / T of feed E1 + 100 g / T of feed E2 (T2), 600 g / T of E1 + 100 g / T of E2 (T3), 1000 g / T of E1 + 100 g / T of E2 (T4), 600 g / T of E1 + 500 g / T of E2 (T5), or 600 g / T of E1 + 500 g / T of E2 + Flavoring (T6) under the conditions of Example 4. The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. figure 13 represents the final live weight at day 35 in grams of broiler chickens fed the normal diet (control: cob + water - T1) or a diet containing cobs mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum(E2) at respectively 200 g / T of feed E1 + 100 g / T of feed E2 (T2), 600 g / T of E1 + 100 g / T of E2 (T3), 1000 g / T of E1 + 100 g / T of E2 (T4), 600 g / T of E1 + 500 g / T of E2 (T5), or 600 g / T of E1 + 500 g / T of E2 + Flavoring (T6) under the conditions of Example 4. The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. figure 14 represents the weight of the thighs at day 35 in grams of broiler chickens fed normal feed (control: cob + water - T1) or feed containing cobs mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum(E2) at respectively 200 g / T of feed E1 + 100 g / T of feed E2 (T2), 600 g / T of E1 + 100 g / T of E2 (T3), 1000 g / T of E1 + 100 g / T of E2 (T4), 600 g / T of E1 + 500 g / T of E2 (T5), or 600 g / T of E1 + 500 g / T of E2 + Flavoring (T6) under the conditions of Example 4. The letters a, b, or c associated with the numbers indicate significant differences between treatments at a 5% confidence level. The percentage values ​​indicate the difference in improvement compared to the control. figure 15 represents the weight of a tibia at day 35 in grams of broiler chickens fed the normal diet (control: cob + water - T1) or a diet containing cobs mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum(E2) at respectively 200 g / T of food from E1 + 100 g / T of food from E2 (T2), 600 g / T of E1 + 100 g / T of E2 (T3), 1000 g / T of E1 + 100 g / T of E2 (T4), 600 g / T of E1 + 500 g / T of E2 (T5) or 600 g / T E1 + 500 g / T of E2 + Flavor (T6) under the conditions of Example 4. The figure 16 represents the total number of bacteria, bifidobacteria spp., lactobacilli spp., enterobacteria, Clostridium jejuni, of Escherichia. Coli-shigella, of salmonella spp and Clostridium perfringens at J35 in the cecum of chickens in log / mg of freeze-dried cecum in broiler chickens fed normal diet (control: cob + water - T1) or a diet containing cobs mixed with protein hydrolysate of Spirulina platensis (E1) and polysaccharide extract of Ascophyllum nodosum(E2) to respectively 200 g / T of E1 + 100 g / T of E2 feed (T2), 600 g / T of E1 + 100 g / T of E2 (T3), 1000 g / T of E1 + 100 g / T of E2 (T4), 600 g / T of E1 + 50 g of E2 (T2) or 600 g / T E1 + 500 g / T E2 + Aroma (T6) under the conditions of Example 4. Example 1 : preparation of extracts Example 1a: Spirulina extract (E1)

[0077] 100g of raw spirulina ( Spirulina platensis)Dehydrated in powder form, having a protein content greater than or equal to 60% and a phycocyanin content greater than or equal to 8%, is extracted by maceration in 900g of water at a temperature of 60°C for a period of between 8 and 6 hours, more advantageously between 2 and 4 hours. The resulting protein extract (liquid) in aqueous solution is hydrolyzed using the serine subtilisin endoprotease enzyme marketed under the trade name Novo-Pro®< D at a temperature of 60°C and a pH of 8 to obtain a spirulina protein hydrolysate.

[0078] The composition of raw spirulina per 100g is summarized in Table 1 below: Table 1 Components g content Water 7,10 Dry matter 92,90 Mineral matter 6,64 Organic matter 86,26 Crude protein 67,50

[0079] The composition of the spirulina protein extract per 100g of raw spirulina is summarized in Table 2 below: Table 2 Components Content in g / l Dry matter 61,0 Mineral matter 8,7 Organic matter 52,3 Proteins 45,2 including Phycocyanin 10,9

[0080] The composition of polypeptides and solubilized peptides of the spirulina protein hydrolysate from 100g of crude spirulina is summarized in the following table 3: Table 3 Constituents as a function of average weight (AM) in Da Content in g / l PM> 20,000 DA 1,4 PM from 10,000 to 20,000 DA 0,6 PM of 5,000 to 10,000 DA 0,4 PM from 1,000 to 5,000 DA 4,4 PM from 500 to 1000 DA 6,8 PM from 150 to 500 DA 14,1 PM < 150 Da 17,5

[0081] The composition of solubilized amino acids (MW < 150 Da) of the spirulina protein hydrolysate from 100g of crude spirulina is summarized in the following table 4: Table 4 Constituents as a function of average weight (AM) in Da Content in g / l Aspartic acid 0,3 Threonine 0,8 Serine 0,9 Glutamic acid 0,7 Proline <0,2 Glycine 0,3 Alanine 1,4 Cystine <0,2 Valine 1,2 Methionine 0,6 Isoleucine 0,8 Leucine 2,4 Tyrosine 1,2 Phenylalanine 1,4 Histidine 0,3 Lysine 1,0 Arginine 1,6 Example 1b: Ascophyllum nodosum extract (E2)

[0082] 200g of Ascophyllum nodosum Crude dehydrated powder is extracted by maceration in 800g of water at a temperature between 45°C and 90°C for a period of 2 to 4 hours. The resulting saccharidic extract (liquid) is clarified by decantation or pressing.

[0083] The composition of Ascophyllum nodosum The gross weight per 100g is summarized in the following table 5: Table 5 Components g content Water 13,5 Dry matter 86,5 Mineral matter 26,4 Organic matter 60,1 Carbohydrates 54,5 Proteins 5,6 Cadmium < 1 ppm Lead < 2 ppm Mercury < 0.02 ppm Fluorine < 75 ppm Arsenic 33.5 ppm

[0084] The composition of the clarified liquid saccharidic extract of Ascophyllum nodosum per 100g of Ascophyllum nodosum The raw data is compiled in the following table 6: Table 6 Constituents Content in g / l Dry matter 143 Mineral matter 50 Organic matter 93 Carbohydrates 86,7 Proteins 6,3 Cadmium < 1 ppm Lead < 2 ppm Mercury < 0.02 ppm Fluorine < 75 ppm Arsenic 5.2 ppm Example 2: Study of the effect of the composition according to the invention on rumen fermentations and the degradation of the ration

[0085] The compositions according to the invention include: A spirulina extract content obtained according to example 1a (E1) corresponding to 50g / day and an extract content of Ascophyllum nodosum obtained according to example 1b (E2) corresponding to 20g / day (50+20g / day E1 + E2); A spirulina extract content obtained according to example 1a (E1) corresponding to 5g / day and an extract content of Ascophyllum nodosum obtained according to example 1b (E2) corresponding to 10g / day (5+10g:E1 + E2); A spirulina extract content obtained according to example 1a (E1) corresponding to 5g / day, an extract content of Ascophyllum nodosumobtained according to example 1b (E2) corresponding to 10g / day and a lithothamnium (CalseaPowder Advance CPA) content corresponding to 80g / day in a simple mixture (CPA + E1 + E2); A spirulina extract content obtained according to example 1a (E1) corresponding to 5g / day, an extract content of Ascophyllum nodosum obtained according to example 1b (E2) corresponding to 10g / d and a lithothamnium content (CalseaPowder Advance CPA) corresponding to 80g / d with impregnation of the extracts on the CPA (CPA + E1 + E2); were tested.

[0086] The comparative compositions include: A spirulina extract content obtained according to example 1a (E1) corresponding to 50g / day or 5g / day (50g / day E1 or 5g / day E1); An extract content of Ascophyllum nodosumobtained according to example 1b (E2) corresponding to 20g / day or 10g / day (20g / day E2 or 10g / day E2); A lithothamnium (CalseaPowder Advance CPA) content corresponding to 80g / day (CPA); A spirulina extract content obtained according to example 1a (E1) corresponding to 5g / day and a lithothamnium (CalseaPowder Advance CPA) content corresponding to 80g / day in a simple mixture (CPA + E1); An extract content of Ascophyllum nodosum obtained according to example 1b (E2) corresponding to 10g / day and a lithothamnium content (CalseaPowder Advance CPA) corresponding to 80g / day in a simple mixture (CPA + E2); A spirulina extract content obtained according to example 1a (E1) corresponding to 5g / day and a lithothamnium content (CalseaPowder Advance CPA) corresponding to 80g / day with impregnation of the extract onto the CPA (CPA + E1); An extract content of Ascophyllum nodosumobtained according to example 1b (E2) corresponding to 10g / d and a lithothamnium content (CalseaPowder Advance CPA) corresponding to 80g / d with impregnation of the extract on the CPA (CPA + E2); were tested. MATERIALS AND METHODS

[0087] The approach is based on fermentation techniques. in vitroDeveloped by the Animal Nutrition Laboratory at the Roullier World Innovation Center, the principle is based on incubating fresh rumen fluid from dairy cows mixed with artificial saliva prepared in the laboratory. The compositions to be tested and the ration (winter type - fibrous) are incorporated under conditions similar to real-life conditions (anaerobic at 39°C) and with continuous agitation (to mimic peristaltic movements). The bacterial flora, in the presence of the appropriate substrate, reproduces ruminal fermentations, allowing for the monitoring of gas production as well as end products such as volatile fatty acids (VFAs) and nitrites (NH3), according to the study objectives.

[0088] To evaluate the effect of the composition according to the invention on rumen fermentation and ration degradation, rumen fluid was collected from three fistulated cows fed a ration based on corn silage, hay, and concentrates (cereal mixture). To reproduce physiological conditions, a ration based on corn silage, hay, and energy concentrate (50:30:20) was incubated in an inoculum composed of rumen fluid and artificial saliva. The rumen fluid / artificial saliva ratio was 1:2. Each trial was conducted over 24 hours with an interim monitoring at 6 hours of fermentation. Finally, the initial pH was adjusted to 6.5 to ensure physiological rumen conditions or to 6.2 to simulate conditions favorable to acidosis, using 80% lactic acid. Each 100 ml flask for incubation consisted of: 20 ml of filtered rumen fluid, 40 ml of artificial saliva (buffering capacity: Buffer substances: sodium and ammonium carbonate; Macro-elements: sodium, phosphorus, potassium, and magnesium; Micro-elements: calcium, manganese, cobalt, and iron), 0.5 g of substrate ground to 1 mm: the substrate can be a winter ration (50% corn silage + 30% hay + 20% energy concentrate (consisting of 20% wheat, 20% corn, 20% barley, 20% beet pulp, 15% wheat bran, and 5% molasses) or an acidogenic ration (33% corn silage + 17% soybean meal + 50% energy concentrate (consisting of 20% wheat, 20% corn, 20% barley, 20% beet pulp, 15% wheat bran, and 5% molasses)). composition to be tested

[0089] After 6 and 24 hours of incubation at 39.5°C, the vials are placed on ice to stop the reactions. The pH is measured directly at the incubator outlet before filtration.

[0090] The substrate is separated from the rumen juice by centrifugation (20 min at 4,000 rpm) to recover 50 ml of juice for the determination of volatile fatty acids (VFAs) in the medium. The recovered substrate is freeze-dried for 48 hours and then ground for fiber determination (Neutral Detergent Insoluble Fiber (NDF) and Acid Detergent Insoluble Fiber (ADF)).

[0091] Continuous pressure monitoring in the flasks allows for the determination of the gas volume produced in ml / g of dry matter (DM). Finally, a collection bag is fitted to the purge port of each gas production recording module to collect a sample for analysis of the gas composition after 24 hours of incubation. RESULTS - Monitoring of gas production:

[0092] Fermentation of the substrate by the rumen flora leads to the production of gases, primarily CO2 and methane (CH4). figures 1 , 3 and 4 present the concentration and volume of CH4 produced after 24 hours of fermentation.

[0093] Extracts E1 and E2 show a synergistic action on CH4 production with reductions of 40% and 36%, respectively for concentration and volume, for the highest application rates. The impact is proportional to the application dose, with a 16% decrease in volume at low doses ( figure 1 These treatments inhibit the fermentative activity of part of the rumen flora.

[0094] The combination of extracts in simple mixture with CPA (lithothamnium) is the most effective treatment for limiting CH4 production (-31% by volume) compared to extracts alone or CPA alone or in mixture with one of the extracts ( figure 3 ). The modalities have a marked effect on these parameters (p<0.001).

[0095] Overall, the combination of extracts impregnated on CPA reduces the volume of CH4 produced during rumen fermentations by 19% ( figure 4 ).

[0096] Thus, during fermentation, the production of CH4 is reduced by the addition of the compositions according to the invention. This reduction confirms the direct inhibition of methanogenic bacteria and protozoa. Indeed, the combination of these two families is responsible for 9 to 25% of CH4 production. The addition of the composition according to the invention should limit energy losses, since CH4 production results in approximately 10% of the energy consumed by the animal being lost. -Ration parameters

[0097] There figure 2This report presents the digestibilities of dry matter (dDM), ADF (dADF), and NDF (dNDF) after 24 hours of fermentation. The synergistic action of the extracts improves ration degradation by 20% to nearly 40%, depending on the application rate and the parameter considered.

[0098] The composition according to the invention thus improves dry matter content (DMC). The ration is therefore better consumed by the rumen flora, whether cellulolytic or amylolytic. The composition according to the invention also increases the digestibility of adipose tissue (ADF) and non-digestive fiber (NDF). It promotes the breakdown and utilization of fiber in the ration, which in this case is rich in forage (80%) with corn silage and hay. CONCLUSION

[0099] The effect of the composition according to the invention on rumen fermentation and ration degradation under physiological conditions was thus evaluated. This composition allows for optimal rumen function through the optimization of ruminal fermentations. It reduces enteric CH4 production. It therefore likely inhibits a portion of the flora, the protozoa associated with methanogenic bacteria. This defaunation limits CH4 emissions, thereby reducing energy losses for the animal, which is essential for the performance of dairy cows.

[0100] The composition according to the invention significantly improves ration degradation. Indeed, the digestibility of dry matter (DM) and fiber (NDF and ADF) increases compared to the control without supplementation. This product allows for better utilization and degradation of fiber by the rumen flora, an effect that is proven and reproducible.

[0101] On the other hand, the use of E1 and E2 alone shows a less significant effect on CH4 production, which clearly demonstrates that the use of these compounds alone is not sufficient to significantly improve microbial metabolism and therefore ration utilization. Example 3 (not part of the invention): Study of the effect of the composition according to the invention on piglets

[0102] The compositions according to the invention include: 0.2% by weight of spirulina (Spirulina platensis) gross and 0.02% by weight of Ascophyllum nodosum crude (T3); 0.2% by weight of spirulina (Spirulina platensis) gross and 0.05% by weight of Ascophyllum nodosum crude (T4); 0.2% by weight of spirulina (Spirulina platensis) gross and 0.1% by weight of Ascophyllum nodosum gross (T5); were tested.

[0103] The comparative compositions include: 0.2% by weight of spirulina (Spirulina platensis) raw (T2); no algae (control T1); were tested. MATERIALS AND METHODS

[0104] Rearing of piglets aged 28 to 70 days, male piglets only. Two buildings: Building 1: 5T × 3C × 18P - Building 2: 5T × 3C × 18P. 51 (Building 1) + 51 (Building 2) = 108 piglets per treatment, for a total of 540 piglets (T = Treatment, C = Cages, and P = Piglets). Distribution of the products throughout the trial period. The piglets are fed a starter feed followed by a grower feed.

[0105] Piglet weaning is characterized by a reduction in feed intake, a period of anorexia lasting 2 to 4 days which, if poorly managed, can lead to intestinal lesions that may produce severe inflammation and tissue damage.

[0106] Controlling intestinal inflammation early is a major challenge in managing intestinal disorders in piglets during this difficult period of their lives.

[0107] Morphometric and functional changes can also be caused by nutritional factors present in certain diets or other products associated with the formulation.

[0108] For example, the use of soybean meal in piglet feed can cause intestinal fermentation and diarrhea. This is because soybean meal contains antigens that induce severe inflammation and tissue damage. For this reason, its inclusion is often restricted in piglet feed. These piglets often have damaged intestinal villi, leading to poor nutrient absorption.

[0109] The objective of this example is to evaluate the effect of supplementation with the composition according to the invention on the immune system (proteins in the blood) and on the morphology of intestinal villi. RESULTS

[0110] Blood immunity : THE figures 5 and 6 These represent the levels of Beta-1-globulin (serum protein) and total protein in the blood of piglets at day 70, respectively. Beta-1-globulin is a polypeptide that exists in a free form and a form bound to cell membranes. This polypeptide plays an important role in immune defenses.

[0111] We observe that the amount of serum protein in the blood increases (p.val = 0.09) with spirulina alone or in a mixture with 0.02% and 0.05% of 'Ascophyllum. As a result, a significant increase of up to 19.7% is observed for the composition containing 0.2% raw Spirulina + 0.02% Ascophyllum raw or 12.8% for the composition containing 0.2% raw Spirulina + 0.05% Ascophyllum Crude. The composition contains 0.2% raw Spirulina + 0.02% Ascophyllum Brute force, therefore, seems to be the most effective.

[0112] Similarly, the total blood protein level increases with spirulina alone or with the composition containing 0.2% raw spirulina + 0.02% Ascophyllum crude. An interesting effect is particularly observed for the T3 composition (0.2% crude Spirulina + 0.02% crude Ascophyllum) with an increase of 4.3%.

[0113] Length of intestinal villi at D70 Numerous morphological studies of the gastrointestinal mucosa have been undertaken to understand the changes in intestinal function during piglet development, particularly around weaning. Weaning induces rapid, transient, and significant changes in piglet physiology, as well as a maturation of intestinal capacities.

[0114] In our study, morphometric measurements were performed at day 70 on small intestine samples, and the results are presented in the figure 7 .

[0115] Supplementation with the composition according to the invention increased the length of the jejunal villi without major effect on the duodenal villi.

[0116] Treatments containing the highest doses of Ascophyllum increase (p.val = 0.08) the length of the jejunum villi up to 15.4% with the composition containing 0.2% crude Spirulina + 0.05% crude Ascophyllum (most interesting composition).

[0117] Supplementation with the composition according to the invention can therefore modulate the inflammatory response away the increase in jejunal villi away the appearance of simple sugars probably or peptides (resulting from enzymatic reactions in specific absorption sites) which thus avoid the damage associated with post-weaning nutritional stress. Conclusions on health and general conclusion:

[0118] Positive effects with the composition according to the invention have been observed, in particular on the length of the jejunal villi of piglets.

[0119] A modulation of the immune response on the integrity of the intestinal mucosa was thus observed in our experiments with an effect of supplementation in composition according to the invention in a breeding of male piglets only from 28 to 70 days of age (post-weaning).

[0120] The results obtained are interesting because daily consumption differs significantly between the different compositions between days 28 and 42 (early childhood period), with a highly significant p-value (0.004), as shown in the... figure 8 .

[0121] Indeed, feed consumption was reduced either due to the transition period (post-weaning) in our test groups, or probably due to the taste or smell of the composition according to the invention; However, despite this reduction in intake, the results remain positive for health, especially for the T3 composition (0.2% crude Spirulina + 0.02% crude Ascophyllum) whose average daily gain (ADG) is equivalent to the control (cf. figure 9 ) while being correlated with a very low daily consumption (-11.8% / T1). Example 4: Study of the effect of the composition according to the invention on broilers

[0122] The compositions according to the invention include: 600 g / T de E 1 + 100 g / T de E 2 1000 g / T de E 1 + 100 g / T E 2 600 g / T E 1 + 500 g / T de E 2 were tested.

[0123] The comparative compositions include: no algae and water (control T1); were tested. MATERIALS AND METHODS

[0124] The feed was formulated to meet 95% of the nutritional requirements of Aviagen's Ross 308 male chickens. It was distributed ad libitum for 35 days. The trial consisted of 6 feeding treatments including cobs and one of the compositions indicated above (T1 to T6). These treatments were administered throughout the rearing period.

[0125] A set of parameters attributable to the intestine were recorded during (days 7 and 15) and at the end of the trial on day 35. RESULTS

[0126] The parameter recorded during the initiation phase (Day 7) and growth phase (Day 15) is the histological study of the intestinal mucosa. The length of the villi and the depth of the crypts in the duodenum, jejunum, and ileum were measured. Thus, the study of intestinal morphology at initiation showed a trend (p < 0.16) towards an increase in the depth of the duodenal crypts at Day 7 ( Figure 11). The depth of the crypts of the chicks in treatment T4 (1000 g / T E1 + 100 g / T E2) shows a 9% increase compared to the control treatment T1. Similar results were observed in the same rearing phase in a previous trial which had indeed demonstrated a trend towards an increase in villus length (p.val = 0.11) and duodenal crypt depth (p.val = 0.15) at day 7, of chicks that received the composition according to the invention in their drinking water ( Figure 12 At the same dose of Ascophyllum extract in water (0.02%), crypts and villi increased as the dose of spirulina extract decreased from 0.1% to 0.02%. Unlike intake from food, it appears that the dose of spirulina extract in drinking water does not need to be maximal to promote villus growth and crypt depth. The dose will therefore depend on the chosen method of administration.

[0127] These variations in villi length and crypt depth are good indicators of nutrient digestion and the small intestine's absorptive capacity. Indeed, and perhaps most importantly, intestinal mucosal development occurs very rapidly after hatching, within the first 7 days of the animal's life. Specifically, this development takes place in the early part of the intestine, in the duodenum, although the rates differ between intestinal segments. Thus, the volume of villi in the duodenum reaches a plateau after 7 days, while it continues to grow in the jejunum and ileum after 7 days. Furthermore, live weight at 42 days of age is correlated with live weight at 21 days, which is itself correlated with live weight at 7 days, which in turn is related to good absorptive capacity early in life.Larger villi, more numerous microvilli, and more active epithelial cells in the duodenum and jejunum early in the lives of chickens suggest a large absorptive surface area and active intestinal function, which allow for faster and better growth of chickens after hatching.

[0128] Performance data (weight and feed intake) were recorded during the finishing period on days 27 and 35. This allowed for the calculation of the feed conversion ratio (FCR) and average daily gain (ADG) of the chickens. Finishing performance measured between days 27 and 35 showed an improvement in ADG from day 27 to day 35 during this period with the introduction of the composition according to the invention ( Figure 12). During the last week of rearing, the addition of the extract mix, in particular the dose of 600 g / T of E1 combined with 100 or 500 g / T of E2, increased the ADG 27-35 by approximately 5.4% compared to the water control (p.val = 0.03). Similarly, this trial demonstrated an increase in final live weight (LW) at day 35 ( Figure 13 Similar to the 27-35 ADG, the live weight at day 35 is improved by approximately 2.7% with a dose of 600 g / T of spirulina extract (E1) combined with a dose of 100 or 500 g / T of Ascophyllum extract (E2). This improvement in final live weight is therefore due to an increase in ADG during the last week of rearing. This suggests that, in the case of feed supplementation, extracts should be administered throughout the entire rearing period to achieve this result.

[0129] To understand the source of this improvement in final body weight (BW), correlations were established between BW and the weight of organs and parts collected and weighed during the trial. A 94% correlation was measured between BW and the weight of chicken thighs. An average correlation of 50% was measured between BW and breast fillets or shins. The meat parts, particularly the thighs and skeleton, therefore appear to explain the weight gain.

[0130] Weighing the most valued cuts for human consumption (fillet and thighs) showed a trend (p.val = 0.17) towards an increase in thigh weight depending on the treatment ( Figure 14 ). There figure 14This shows that chickens receiving 600 g / t of E1 + 100 g / t of E2 had a thigh weight almost 3% higher than the water control. Chickens in this treatment, slightly heavier at the end of the rearing period, also had a higher thigh weight than chickens in the other treatments.

[0131] As shown by figure 15The weight of the tibias also tended to increase (p.val = 0.18) by almost 4% with the addition of 600 g / T of E1 + 100 g / T of E2. Such a predisposition is relatively significant in broiler chickens, whose genetics, carefully selected over the last few decades, have resulted in improved growth capacity. Indeed, the growth rate of chickens has increased by 300% in fifty years, from 25 g / day to 100 g / day. This progress, however, has led to weakened legs, too weak to support such weight so quickly. After forty days of rearing, this can represent nearly 30% of chickens exhibiting locomotion problems or an inability to walk. The growth of these animals is then slowed, as the chickens no longer have access to the feed. Indirectly, these problems represent an economic loss for farmers.It is therefore important that the bone structure of the tibias be of good quality since this is a component that will affect the farmers' wages.

[0132] The parameters recorded during the finishing phase (Day 35) or at felling are: At slaughter, samples of cecal contents were also collected to count the following bacterial populations by qPCR: Beneficial populations: Lactobacilli, Bifidobacteria; Pathogenic populations: Clostridium perfringens Salmonella spp Escherichia. Coli-shigella Clostridium jejuni Enterobacteriaceae Total Bacteria

[0133] The bacterial populations of the cecum were enumerated. The results showed changes in these populations with the addition of the composition according to the invention ( Figure 16 ).

[0134] There figure 16This shows that chickens receiving 600 g / T of E1 + 100 g / T of E2 (T3) exhibit the lowest bacterial population development. This is particularly true for bifidobacteria, lactobacilli, and enterobacteria. E. coli-shigella and salmonella.

[0135] Therefore, the total number of bacteria in the cecum is 8.59 Log10 for chickens in this treatment, whereas this number ranges from 8.60 to 9.05 Log10 for the other treatments. The flavoring treatment is not recommended here as it promotes the growth of these populations. A discriminant analysis coupled with a comparison of distances from this analysis using Fisher's exact test revealed that the populations in the control and treatment T3 groups are significantly different (p = 0.04). Furthermore, we note that all the effects observed so far between treatments T2, T3, and T4, at a constant dose of extracts of AscophyllumHowever, increasing doses of spirulina extract do not follow a linear regression (improvement of parameters with increasing spirulina extract dose) but rather a quadratic plateau regression. Therefore, the spirulina extract dose does not need to be maximal for effects to be observed. Conversely, too high a dose of spirulina extract could diminish the results. General Conclusion

[0136] The trial presented here, supplemented by results from a previous trial, demonstrated the benefit of supplementing with either a dose of 0.02% spirulina extract + 0.02% extract AscophyllumIn the drinking water of chickens from day 0 to day 7, a dose of 1000 g of spirulina extract + 100 g of spirulina extract per 100 g of chicken feed of the same age is recommended. A starter supplement could indeed be considered, as it promotes increased villous length and duodenal crypt depth. This effect is also a good indicator of nutrient digestion and small intestine absorptive capacity, functions that are relatively important at the start of the breeding cycle because they can influence the animals' weight gain and the modulation of immunity throughout their lives. The recommended dose will depend on the form of administration: in the drinking water or in the feed.

[0137] Similarly, this feed supplement could continue to be administered until the end of the rearing period at a dose of 600 g of spirulina extract + 100 g of Ascophyllum extract per gram of feed to achieve a higher final body weight (BW) due to increased leg weight. This dose also results in a higher tibia weight, which promotes better locomotion and reduces total cecal bacterial populations, including pathogenic bacteria. E. coli shigella, Enterobacteriaceae and salmonella. Example 5 : Study of the effect of the composition according to the invention on rumen fermentations and the degradation of the ration

[0138] The compositions according to the invention include: an excerpt of Ascophyllum nodosum obtained according to example 1b (AN- E); an extract of Spirulina platensis obtained according to example 1a (SP-E); a mixture (SAT-E) of 32% by weight of spirulina extract obtained according to example 1a (E1) and 68% by weight of extract of Ascophyllum nodosum obtained according to example 1b (E2); of the Spirulina platensisin raw form (SP-B); of Ascophyllum nodosum in crude form (AN-B); a mixture (SAT-B) of 32% by weight Spirulina platensis under raw form and 68% by weight of Ascophyllum nodosum in raw form.

[0139] The doses tested for each mixture ranged from 1 to 30 g / day (low). The method is the same as in Example 1 (fermentation technique). in vitro developed by the Animal Nutrition laboratory of the Roullier World Innovation Centre). RESULTS :

[0140] They are summarized in Tables 7 and 8 below: Table 7: effects on methane production dose [CH 4 ] % Volume of CH 4 (ml / g of DM) diff in [CH 4 ] volume difference Witness None 4,4 6,5 AN-B Bass 4,1 6,24 -5% -4% SAT-B Bass 3,1 4,83 -29% -26% SP-B Bass 3,9 5,69 -10% -12% AN-E Bass 4,2 6,20 -4% -5% SAT-E Bass 3,7 5,75 -15% -12% SP-E Bass 4,2 5,92 -4% -9%

[0141] A significant effect of the low application dose (<30g / l) is observed: p<0.001 on the CH4 concentration of the gas for the mixture which is higher than that of the algae alone, which clearly demonstrates a synergy.

[0142] The mixture of raw algae is even more interesting than the mixture of algae extracts (-29% on concentration and -26% on volume). Table 8: effects on the digestibility of the ration (dMS): dose dMS % diff vs. control Witness None 49 AN-B Bass 49 0% SAT-B Bass 54,2 11% SP-B Bass 50,3 3% AN-E Bass 49,1 0% SAT-E Bass 53,2 8% SP-E Bass 48,5 -1%

[0143] The composition according to the invention, containing the algae mixture according to the invention, whether in their raw or extracted form, shows a slight improvement in dMS compared to algae alone. The synergistic effect will potentially be more pronounced in in person with daily consumption.

[0144] Furthermore, no negative effects on fermentation were observed at the tested doses.

Claims

1. A composition for the nutrition or drink of a non-human farm animal, advantageously a ruminant animal and / or a monogastric animal such as a poultry or a pig and / or an aquatic animal such as a fish or a crustacean, comprising the combination of a protein hydrolyzate of spirulina and polysaccharide extract of Ascophyllum nodosum.

2. The composition according to claim 1, characterized in that the polysaccharide extract of Ascophyllum nodosum is obtained by extraction in an aqueous solvent.

3. The composition according to any one of claims 1 or 2, characterized in that the protein hydrolyzate of spirulina / polysaccharide extract of Ascophyllum nodosum weight ratio, is comprised within the range 0.02-100, advantageously 0.2 - 15, in particular 0.5 - 20.

4. The composition according to any one of claims 1 to 3, characterized in that it comprises between 0.01 and 0.5% by weight relative to the total weight of the composition, advantageously between 0.02 and 0.2% by weight relative to the total weight of the composition, of the protein hydrolyzate of spirulina.

5. The composition according to any one of claims 1 to 4, characterized in that it comprises between 0.05 and 0.5% by weight relative to the total weight of the composition, advantageously between 0.01 and 0.1% by weight relative to the total weight of the composition, of Ascophyllum nodosum, advantageously of Ascophyllum nodosum extract.

6. The composition according to any one of claims 1 to 5, characterized in that it further comprises lithothamnium.

7. A use of the composition according to any one of claims 1 to 6, for improving the zootechnical performance of a ruminant and / or an omnivore for example a pig.

8. The use of the composition according to any one of claims 1 to 6, in order to reduce the emission of greenhouse gases, in particular methane, in a ruminant animal.

9. The composition according to any one of claims 1 to 6, for its use for improving the immune system in non-human farm animals, advantageously ruminant animals and / or monogastric animals such as birds, for example poultry, or omnivore animals, for example pigs, and / or aquatic animals such as fish or crustaceans, in particular in chickens, chicks, pigs or piglets.

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