Process for extracting a fat from animal fat

Abstract

The invention relates to a process (100) for extracting an oxygenated fat from animal fat, characterized in that the animal fat is derived from a reptile and that said extraction process (100) comprises a step (130) of extraction by a supercritical carbon dioxide extractor at a given extraction temperature and a given extraction pressure. Figure to be published with the abstract: Fig. 1

Description

Title of the invention: Process for extracting a fat from animal fat. Technical field

[0001] The present invention relates generally to the field of oxygenated fat extraction. It relates in particular to fats derived from animal fat, and especially to a group of oxylipins. Previous technique

[0002] Oxygenated fats constitute a family of oxygenated natural products formed from fatty acids via pathways involving at least one oxygen-dependent oxidation step. Among the molecules of interest are phosphatidylethanolamines, phosphatidylcholine, phosphatidylinositol, phosphatidylserine, sphingomyelin, and, in particular, oxylipins. Oxylipins are derived from polyunsaturated fatty acids by the enzymes cyclooxygenase (COX), lipoxygenase (LOX), or cytochrome P450 epoxygenase. Oxylipins, thus produced from fatty acids, are found in numerous organisms such as microorganisms (algae and others), plants, and animals, and are known to have various biological functions.

[0003] Reptile fat has been used for a very long time by indigenous peoples in direct application to the skin for the healing of dermatological problems. Based on this natural observation, the present inventor has identified, through specific and in-depth research, that oxylipins are found in quantity and quality in reptile fat, particularly crocodile fat, with the identification of fourteen oxygenated compounds (oxylipins) in the latter, namely: 7-HDOHE (7-Hydroxydocosahexaenoic acid); 14-HDOHE (Docosahexaenoic acid); 15-HETE (15-Hydroxyeicosatetraenoic acid); 17-HDOHE (17-Hydroxydocosahexaenoic acid); 8-HETE (8-Hydroxyeicosatetraenoic acid); 4-HDOHE (4-Hydroxydocosahexaenoic acid); 12-HETE (12-Hydroxyeicosatetraenoic acid); 11-HDOHE (Docosahexaenoic acid); 5-HEPE (5-Hydroxyeicosapentaenoic acid); 5-HETE (5-5S-Hydroxyeicosatetraenoic acid);15-HEPE (15S-hydroxyeicosapentaenoic acid); 12-HEPE (12-hydroxyeicosatetraenoic acid); 5-oxoETE (5-oxo-eicosatetraenoic acid) and 12-oxoETE (12-oxo-eicosatetraenoic acid) originate primarily from the metabolism of three acids: arachidonic acid, dicosohexanoic acid, and dicosopentanoic acid. These oxygenated fats are considered high-value products due to their bioactive properties and; of their potential in the development of therapeutic, cosmetic and food products with a wide range of biological functions, such as apoptosis, tissue repair, blood clotting, cell proliferation, blood vessel permeability, pain regulation, inflammation regulation, immune actions, blood pressure regulation and / or fat metabolism regulation.

[0004] Although no method for extracting oxygenated fats from reptile fat has been demonstrated, it is traditionally known in industry to use liquid organic solvents to extract fats from natural substrates, which would encourage the use of this type of extraction method. However, due to environmental and health concerns, these solvents, considered by many to be toxic and harmful to health and the environment, are increasingly losing their appeal in processing industries as extraction agents. Manufacturers are therefore increasingly turning to solvents considered "good solvents," such as ethanol or methanol. However, extraction using organic solvent(s) requires a subsequent distillation step to remove the organic solvent(s) from the extracted fractions.However, the high temperature required to distill the last traces of solvent(s) can degrade the resulting extract. Description of the invention

[0005] The present invention therefore aims to improve upon this observation. To this end, it proposes a process for extracting oxygenated fatty substances from reptile fat using environmentally and health-friendly components and compounds, ensuring controlled and pure extraction, specifically preserving the integrity of the resulting extract. The extract obtained can then be used in pharmacological, cosmetic, and / or food products.

[0006] To this end, a first object of the invention relates to a process for extracting an oxygenated fat from animal fat obtained from a reptile, said extraction process comprising an extraction step using a supercritical carbon dioxide extractor at a given extraction temperature and a given extraction pressure. Indeed, in its supercritical state, carbon dioxide possesses very particular properties: high diffusivity and a high density, which gives it a significant transport and extraction capacity. Furthermore, with a low critical temperature, on the order of thirty-one degrees Celsius, supercritical carbon dioxide makes it possible to process heat-sensitive products.

[0007] Preferably, the oxygenated fat body is a group of oxylipins.

[0008] Preferably, the reptile is a crocodile.

[0009] Advantageously, the extraction temperature is between thirty-five degrees Celsius and ninety degrees Celsius.

[0010] The extraction pressure is preferably between seventy-four bars and four hundred bars.

[0011] According to a first embodiment of the invention, said process for extracting a fat from animal fat comprises: - a step of obtaining a first mixture by mixing a given quantity of animal fat with a given quantity of solvent or mixture of solvents, the latter having a weight greater than or equal to the weight of the quantity of animal fat; - a step of obtaining a second mixture by mixing a first given quantity of anti-clogging agent with the first mixture obtained; - the extraction step by said supercritical carbon dioxide extractor consisting of extracting the second mixture obtained with a second given quantity of anti-clogging agent so as to obtain at least one lipophilic phase and one hydrophilic phase, each of these phases containing a given concentration of said oxygenated fat.

[0012] Advantageously, the first given quantity of anti-clogging agent has a weight equivalent to five times said weight of the given quantity of animal fat.

[0013] Said anti-clogging agent may be derived from a set of anti-clogging agents comprising perlite, diatomite, sand, anthracite, activated carbon, and grafted silica.

[0014] Said solvent or said mixture of solvents may be derived from a set of solvents comprising ethanol, methanol, isopropanol, and dimethyl ether.

[0015] Said extraction process according to the first embodiment may further include steps prior to the step of obtaining a first mixture consisting of: - a step of grinding the animal fat; - a step of mixing the ground animal fat and; - a storage stage of ground animal fat at a temperature below or equal to zero degrees.

[0016] Said extraction process according to the first embodiment may further comprise subsequent steps to the extraction step consisting of: - a step to preserve the lipophilic phase consisting of adding an antioxidant to said lipophilic phase; - a step of preserving the hydrophilic phase consisting of adding a preservative to said hydrophilic phase.

[0017] Said extraction process according to the first embodiment may further comprise subsequent steps to the extraction step consisting of: - a vacuum conditioning step of the hydrophilic phase at a temperature less than or equal to forty-five degrees Celsius; - a step of obtaining said oxygenated fat consisting of mixing the conditioned hydrophilic phase with the lipophilic phase so as to obtain an emulsion containing a given concentration of said oxygenated fat.

[0018] According to a second embodiment of the invention, said process for extracting a fat from animal fat comprises: - a solvent extraction step of a given quantity of animal fat in order to obtain an extraction solute; - the extraction step by said supercritical carbon dioxide extractor consisting of extracting the extraction solute obtained, so as to obtain a hydrophilic phase containing a given concentration of said oxygenated fat.

[0019] Said extraction process according to the second embodiment may further comprise steps prior to the solvent extraction step consisting of: - a step of grinding animal fat; - a step of mixing the ground animal fat and; - a storage stage of ground animal fat at a temperature below or equal to zero degrees.

[0020] Said extraction process according to the second embodiment may further comprise subsequent steps to the extraction step by said supercritical carbon dioxide extractor, consisting of: - a vacuum conditioning step of the hydrophilic phase at a temperature less than or equal to forty-five degrees Celsius; - a step of preserving the hydrophilic phase consisting of adding a preservative to said hydrophilic phase.

[0021] Finally, a second object of the invention relates to a cosmetic product comprising a fatty body obtained from the extraction process according to the first or second embodiment according to the invention. Brief description of the drawings

[0022] The invention will be better understood and other features and advantages thereof will become apparent from the following description of particular embodiments of the invention, given by way of illustrative and non-limiting examples, and with reference to the accompanying drawings, among which: - [Fig.1] shows a preferred example of a process according to a first embodiment of the invention; - [Fig.2] shows a first variant of the embodiment of the example presented in [Fig.1] according to the invention; - [Fig.3] shows a second variant of the embodiment of the example presented in [Fig.1] according to the invention; - [Fig.4] a preferred example of a process according to a second embodiment of the invention. Description of the implementation methods

[0023] To simplify the description, the same reference numeral is used in different figures to designate the same process step or the same element. Thus, when the description refers to a process step or a referenced element, this process step or element can be identified in several figures. Furthermore, the figures and the description are given as non-limiting examples of embodiments.

[0024] A method for extracting oxygenated fat according to the invention consists of extracting oxygenated fat from animal fat using a commercially available supercritical carbon dioxide extractor, for example, the ExtrateX SFE 25-liter model or the LABOAO LSFE-221-50-06 model. Such an extractor operates in a closed circuit and includes pressure-control elements, such as pumps, and temperature control elements, such as heat exchangers, to bring the carbon dioxide above its critical point, in this case more than seventy-four bar and thirty-one degrees Celsius. Thus, such a method comprises an extraction step using a supercritical carbon dioxide extractor at a given extraction temperature and pressure.This step involves placing the product to be treated, in this case animal fat, into the extractor, which is traversed by a flow of supercritical carbon dioxide. The supercritical carbon dioxide fluid becomes saturated with the extracted compound, then it is expanded, transitions to a gaseous phase, and separates from the extracted compound. The latter is then collected in a separator. This extraction process eliminates all undesirable lipophilic substances from the product being treated, retaining only the desired fat(s).

[0025] For illustrative but not limiting purposes, such a process according to the invention is advantageously described herein and implemented using crocodile fat, and the extracted oxygenated fatty substance is advantageously a group of oxylipins. Such crocodile fat may be obtained from a liposuction step on a crocodile, in this case an aspiration step of fat deposits from a crocodile, or from the legal and controlled slaughter of a crocodile.

[0026] In order to optimize the extraction process for the intended application, the extraction temperature is advantageously between thirty-five degrees Celsius, a temperature above the critical temperature, and ninety degrees Celsius, corresponding to a temperature ensuring non-deterioration and maintenance of the active principles of the extracted compound, preferably at sixty degrees Celsius. As for the extraction pressure, it is advantageously between seventy-four and four hundred bars, preferably two hundred bars.

[0027] As mentioned previously, the invention consists of extracting an oxygenated fatty substance from an animal fat.

[0028] A preferred example, corresponding to a first embodiment, of a process 100 for extracting an oxygenated fatty substance from an animal fat, according to the invention, is shown in [Fig.1].

[0029] Thus, such a process 100 comprises a first step 110 consisting of obtaining a first mixture Ml. This first mixture Ml is obtained by mixing a given quantity of animal fat with a given quantity of a solvent or a mixture of solvents. The quantity of solvent or mixture of solvents has a weight Ps greater than or equal to the weight Pg of the quantity of animal fat. Examples of solvent(s) will be given in the remainder of this document.

[0030] In order to make the extraction process 100 as efficient as possible, particularly by providing a better yield, said process may optionally include a preliminary step to step 110 of obtaining the first mixture Ml, in this case a step 101 of grinding the animal fat. Such a step 101 has the effect of fractionating the animal fat and reducing the particles, thereby improving the yield during the extraction step. To this end, by way of illustration but not limitation, it may be considered, for example, to use a knife mill operating at five thousand revolutions per minute. In addition, such a grinding step 101 may be followed by a step 102 of mixing the ground animal fat in order to promote a homogeneous mixture of animal fat.Furthermore, in the event that the delay between step 101 of grinding the fat or step 102 of mixing the fat and step 110 of obtaining the first mixture Ml is relatively long because said steps are not, for example, carried out on the same day and / or in the same place, a subsidiary step 103 of storing the ground animal fat at a temperature less than or equal to zero degrees Celsius may possibly be carried out.

[0031] A second step 120 of said process 100 consists in obtaining a second mixture M2. This second M2 is obtained by mixing a first given quantity Q1 of an anti-clogging agent with the first mixture M1 obtained in the first step 110. Said first quantity Q1 of anti-clogging agent must be adapted according to the type of anti-clogging agent used. By way of illustration, this quantity Q1 may advantageously have a weight equivalent to five times said weight Pg of the given quantity of animal fat. However, such a ratio between the weight associated with the quantity Q1 of anti-clogging agent and the weight Pg of the quantity of animal fat is not limited but is determined according to the type of agent Selected anti-clogging agent. Examples of anti-clogging agents will be given in the remainder of this document.

[0032] A third step 130 of said process 100 consists of the aforementioned extraction step, using said supercritical carbon dioxide extractor, at a given extraction temperature and pressure so as to obtain at least one lipophilic phase PLI and one hydrophilic phase PHI, each containing a given concentration of oxygenated fat. Such a step 130 thus makes it possible to remove any non-oxygenated fat. For such a first embodiment, such a step 130 consists of extracting, in said supercritical carbon dioxide extractor, the second mixture M2 obtained in the preceding step 120 in the presence of a second given quantity Q2 of anti-clogging agent. To do this, said second given quantity Q2 of anti-clogging agent and the second mixture M2 are added to said extractor.The extractor is then closed and locked, and the extraction cycle can begin after setting the extraction parameters, namely temperature, pressure, and extraction time. As a reminder, for illustrative purposes, the extraction temperature is advantageously between thirty-five and ninety degrees Celsius, and the extraction pressure is advantageously between seventy-four and four hundred bars. The extraction time is advantageously between ten and one hundred and eighty minutes. Preferably, to promote better yield, the given quantity Q2 of anti-clogging agent can be halved before being added to the extractor and thus added in two stages according to the following protocol: - adding the first half of the quantity Q2 of anti-clogging agent into said extractor; - addition of the second mixture M2 into said extractor; - adding the second half of the quantity Q2 of anti-clogging agent into said extractor. At the end of this step 130, an extract is obtained, corresponding to a lipophilic phase PLI, in this case an oily phase, and a hydrophilic phase PHI, in this case an aqueous phase, each containing a high concentration of said oxygenated fatty body.

[0033] The extract (the lipophilic PLI and / or hydrophilic PHI phase) can thus be used as is or after a purification step to retain only the desired oxygenated fatty substance for cosmetic or other applications by incorporating said extract, in its purified form, into the composition of a cosmetic product. Advantageously, to prevent any deterioration and / or premature aging of the extract, as illustrated in [Fig. 2], said process 100 may include optional steps for preserving said PLI and PHI phases obtained, such as: - a step 140 for preserving the lipophilic PLI phase, consisting of adding an antioxidant to said lipophilic PLI phase to prevent its premature oxidation. By way of illustration but not limitation, the antioxidant agent used may be: i. a synthetic primary antioxidant such as butyl hydroxytoluene, butylated hydroxyanisole, ethoxyquin, tertiary butyl hydroquinone or propyl gallate; ii. a natural primary antioxidant such as lutein, vitamin E (tocopherols), cambogia, carnosic acid or other plant extracts; iii. A secondary antioxidant such as citric acid, phosphoric acid, or ethylenediaminetetraacetic acid, also known as EDTA. Optionally, such an antioxidant can also be incorporated into the animal fat during grinding step 101 to stabilize and protect the ground fat from oxidation. - a 150 preservation step of the hydrophilic phase PHI consisting of adding a preservative to said hydrophilic phase PHI. By way of illustration but not limitation, the preservative used may be derived from various aqueous phase preservatives, such as leuconostokes, radish root filtrate, benzoic acid, sorbic acid, benzyl acid, dehydroacetic acid, potassium sorbate.

[0034] Alternatively, as illustrated in [Fig. 3], when the extraction purification step can prove lengthy and costly, it is possible to obtain an emulsion, in this case a mixture of an oily phase with an aqueous phase, containing a given concentration of oxygenated fat. To this end, said process 100 may comprise two steps subsequent to the extraction step 130, in this case: - a first step 160 of vacuum conditioning of the hydrophilic phase PHI at a given temperature, advantageously less than or equal to forty-five degrees Celsius, such a step 160 allowing the evaporation of the solvent or mixture of solvents so as to obtain a maximum concentration of oxygenated fats; - and a second step 170 of obtaining said oxygenated fat consisting of mixing the hydrophilic phase previously conditioned in step 160 with the lipophilic phase PLI.

[0035] A second example, corresponding to a second embodiment, of a process 200 for extracting an oxygenated fat from animal fat, according to the invention, is shown in [Fig. 4]. Thus, such a process 200 comprises a first step 210 of solvent extraction, solid-liquid extraction, of a given quantity of animal fat so as to obtain an extraction solute SE, otherwise known as miscella. This first step 210 makes it possible to concentrate a first portion of the oxygenated fats. To do this, solvent extraction relies on the solubility of the species to be extracted in a given solvent. The solvent must then The solvent must be immiscible with water, readily dissolve the substance to be extracted, and be liquid at the extraction temperature. Such a solvent extraction step 210 begins with a first substep of macerating a given quantity of animal fat in a pre-selected solvent, according to the aforementioned criteria. For this purpose, equipment such as an incubation hood can be used to control the extraction temperature and agitation; for example, the TH 15 hood from Edmund Buhler GmbH. This is followed by a second substep of filtration, for example, through filter paper, with the miscella being collected at the end of this second substep.

[0036] The process 200 includes a second step 220 consisting of the aforementioned extraction step, using said supercritical carbon dioxide extractor, at a given extraction temperature and pressure in order to remove any non-oxygenated fats from the extraction solute SE. For such a second embodiment, the extraction solute SE obtained in step 210 is thus integrated into said extractor. The extractor is then closed and locked, and the extraction cycle can thus start after setting the extraction parameters, namely the temperature, pressure, and extraction time. As with the first embodiment, in this case the extraction process 100, the extraction temperature is advantageously between thirty-five and ninety degrees Celsius, and the extraction pressure is advantageously between seventy-four and four hundred bars.The extraction time is advantageously between ten and one hundred and eighty minutes. At the end of this step 220, a hydrophilic phase PH2 containing a given concentration of oxygenated fat is obtained.

[0037] As with process 100, said process 200 may optionally include steps prior to solvent extraction step 210, in this case a step 201 of grinding the animal fat, followed or not by a step 202 of mixing the ground animal fat, followed or not by a step 203 of storing the ground animal fat at a temperature below or equal to zero degrees Celsius. Such steps 201, 202 and 203 have the same effects and characteristics as the aforementioned steps 101, 102 and 103.

[0038] In addition, to obtain a maximum concentration of oxygenated fats, an additional step may be carried out after step 220 of extraction by said carbon dioxide extractor, in this case a vacuum conditioning step 230 of the hydrophilic phase PH2 at a given temperature, advantageously less than or equal to forty-five degrees Celsius, thus allowing the evaporation of any solvent residue. Furthermore, to prevent any deterioration and / or premature aging of the hydrophilic phase PH2 obtained, said process 200 may also include an optional preservation step corresponding to a Step 240 of preservation of the hydrophilic phase PH2. Such a step 240 consists of adding a preservative to said hydrophilic phase PH2: the preservative used may be from various aqueous phase preservatives, such as benzoic acid, sorbic acid, benzyl acid, dehydroacetic acid, potassium sorbate; leuconostocs, or radish root filtrate.

[0039] By way of illustration but not limitation, throughout this document, when reference is made to the use of: - an anti-clogging agent, which may be perlite, diatomite, sand, anthracite, activated carbon in various grades, or grafted silica in various grades. However, a person skilled in the art should not limit themselves to this list of anti-clogging agents but could use any other type of material that prevents clogging of the grease in the extractor; - a solvent or a mixture of solvents; the solvent may be ethanol, methanol, isopropanol, or dimethyl ether. However, a person skilled in the art should not limit themselves to this list of solvents but could use any other type of solvent fulfilling the functions mentioned herein.

[0040] Another object of the present invention relates to a cosmetic product comprising an oxygenated fatty body obtained from the extraction process 100 or 200 according to the invention.

[0041] It will be appreciated by those skilled in the art that this disclosure is not limited to what is particularly shown and described above. Other modifications may be envisaged without departing from the scope of the present invention as defined by the annexed claims. In this instance, throughout the present disclosure, the extraction processes described apply to oxygenated fats derived from animal fat, but such extraction processes could be applied to fats derived from vegetable oils, for example.

Claims

Demands

1. Extraction process (100, 200) of an oxygenated fat from an animal fat, characterized in that the animal fat is derived from a reptile and that said extraction process comprises an extraction step (130, 220) by a supercritical carbon dioxide extractor at a given extraction temperature and extraction pressure.

2. A method for extracting (100, 200) an oxygenated fat from an animal fat according to the preceding claim, wherein the oxygenated fat is a group of oxylipins.

3. A method for extracting (100, 200) an oxygenated fatty substance from an animal fat according to any one of the preceding claims, wherein the reptile is a crocodile.

4. A method for extracting (100, 200) an oxygenated fat from an animal fat according to any one of the preceding claims, wherein the extraction temperature is between thirty-five degrees Celsius and ninety degrees Celsius.

5. A method for extracting (100, 200) an oxygenated fat from an animal fat according to any one of the preceding claims, wherein the extraction pressure is between seventy-four bars and four hundred bars.

6. A process (100) for extracting an oxygenated fat from animal fat according to any one of the preceding claims, said process (100) comprising: - a step (110) of obtaining a first mixture (Ml) by mixing a given quantity of animal fat with a given quantity of solvent or solvent mixture, the latter having a weight (Ps) greater than or equal to the weight (Pg) of the quantity of animal fat; - a step (120) of obtaining a second mixture (M2) by mixing a first given quantity (Ql) of anti-clogging agent with the first mixture (Ml) obtained; - the extraction step (130) by said supercritical carbon dioxide extractor consisting of extracting the second mixture (M2) obtained with a second given quantity (Q2) of anti-clogging agent so as to obtain at least one lipophilic phase (LPP) and one phase hydrophilic (PHI), each of these phases (PLI, PHI) containing a given concentration of said oxygenated fat.

7. A method for extracting (100) an oxygenated fat from an animal fat according to the preceding claim, wherein the first given quantity (Ql) of anti-clogging agent has a weight equivalent to five times said weight (Pg) of the given quantity of animal fat.

8. A process for extracting (100) an oxygenated fat from an animal fat according to any one of claims 6 to 7, wherein the anti-clogging agent is taken from a set of anti-clogging agents comprising perlite, diatomite, sand, anthracite, activated carbon, and grafted silica.

9. A process for extracting (100) an oxygenated fat from an animal fat according to any one of claims 6 to 8, wherein the solvent or mixture of solvents is taken from a set of solvents comprising ethanol, methanol, isopropanol, and dimethyl ether.

10. A process for extracting (100) an oxygenated fat from an animal fat according to any one of claims 6 to 9, further comprising steps prior to step (110) of obtaining a first mixture consisting of: - a step (101) of grinding the animal fat; - a step (102) of mixing the ground animal fat and; - a step (103) of storing the ground animal fat at a temperature below or equal to zero degrees.

11. A process for extracting (100) an oxygenated fat from an animal fat according to any one of claims 6 to 10, further comprising subsequent steps to the extraction step (130) consisting of: - a step (140) for preserving the lipophilic phase (PLI) consisting of adding an antioxidant to said lipophilic phase (PLI); - a step (150) for preserving the hydrophilic phase (PHI) consisting of adding a preservative to said hydrophilic phase (PHI).

12. A process for extracting (100) an oxygenated fat from an animal fat according to any one of claims 6 to 11, comprising in in addition to subsequent steps to the extraction step (130) consisting of: - a step (160) of vacuum conditioning of the hydrophilic phase (HPP) at a temperature less than or equal to forty-five degrees Celsius; - a step (170) of obtaining said oxygenated fat consisting of mixing the conditioned hydrophilic phase (HPP) with the lipophilic phase (LPP) so as to obtain an emulsion containing a given concentration of said oxygenated fat.

13. A process for extracting (200) an oxygenated fat from an animal fat according to any one of claims 1 to 5, said process comprising: - a solvent extraction step (210) of a given quantity of animal fat so as to obtain an extraction solute (SE); - the extraction step (220) by said supercritical carbon dioxide extractor consisting of extracting the extraction solute (SE) obtained, so as to obtain a hydrophilic phase (PH2) containing a given concentration of said oxygenated fat.

14. A process for extracting (200) an oxygenated fat from an animal fat according to the preceding claim, further comprising steps prior to the solvent extraction step consisting of: - a step (201) of grinding the animal fat; - a step (202) of mixing the ground animal fat; and - a step (203) of storing the ground animal fat at a temperature below or equal to zero degrees.

15. A process for extracting (200) an oxygenated fat from an animal fat according to any one of the preceding claims 13 to 14, further comprising subsequent steps to the extraction step (220) by said supercritical carbon dioxide extractor consisting of: - a step (230) of vacuum conditioning the hydrophilic phase (PH2) at a temperature less than or equal to forty-five degrees Celsius; - a step (240) of preserving the hydrophilic phase (PH2) consisting of adding a preservative to said hydrophilic phase (PH2).

16. Cosmetic product characterized in that it comprises a fatty substance obtained from the extraction process (100, 200) according to any one of claims 1 to 15.

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