Compositions and method for making succinic acid

Abstract

The present invention discloses an engineered Corynebacterium strain and a process of making succinic acid in microaerobic conditions. The process of making succinic acid as disclosed in the present invention utilizes carbon dioxide as secondary carbon source during production phase and replaces bicarbonate as a source of secondary carbon. Further, integration of gas recycle and the biomass recycle in the process made it sustainable while having minimal or no loss in specific productivity for succinic acid production. The present invention has the advantage of yielding higher succinic acid levels while reducing resource costs.