A neuroinflammation lumbar puncture cerebrospinal fluid collection device with anti coagulation function

Abstract

This invention discloses a lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammation, belonging to the field of medical device technology. It addresses the problem of unsatisfactory anti-coagulation effects. The device includes a base, with a rotating shaft rotatably connected to the inner cavity of the base via bearings. A driving mechanism is provided on the surface of the rotating shaft. Support plates are fixedly connected to both sides of the top of the rotating shaft. A positioning frame is fixedly connected to the top of one of the two support plates on opposite sides. Collection bottles are evenly inserted into the inner surface of the positioning frame. This invention, by setting up collection bottles and sealing plugs, can collect and store lumbar puncture cerebrospinal fluid. The positioning frame facilitates the positioning of the collection bottles. By setting up an electric push rod, push plate, toothed plate, and gears, the rotating shaft, positioning frame, and collection bottles can be driven to swing slightly. The liquid, relying on inertia and gravity, forms a wave-like flow or overall horizontal movement within the bottle, achieving gentle mixing with zero shear force and preventing cerebrospinal fluid coagulation.

Description

Instruction Manual 1 A Cerebrospinal Fluid Collection Device for Lumbar Puncture in Neuroinflammation with Anti-coagulation Function Technical Field This invention relates to the field of medical device technology, and in particular to a cerebrospinal fluid collection device for lumbar puncture in neuroinflammation with anti-coagulation function. Background Art Neuroinflammation is a common disease of the central nervous system, and its diagnosis and treatment often rely on cerebrospinal fluid examination. Lumbar puncture is the standard method for obtaining cerebrospinal fluid. In clinical practice, the cerebrospinal fluid obtained through lumbar puncture needs to be collected in a container for biochemical, microbiological, and cytological analysis. Existing cerebrospinal fluid collection devices typically include a simple test tube or collection bottle. Medical personnel drain the cerebrospinal fluid into the bottle through a catheter. However, in actual operation and application, these traditional devices have significant shortcomings, such as the lack of an effective anti-coagulation mechanism. Specifically, after cerebrospinal fluid (CSF) is removed from the body, the ambient temperature (especially winter room temperature or air-conditioned environments) is usually much lower than the body temperature. This causes proteins (such as total protein and oligoclonal band-related proteins) and inflammatory cells to easily coagulate, precipitate, or adhere to the fluid wall. This problem is particularly prominent in patients with neuroinflammation, as their CSF protein content is often significantly elevated, leading to faster coagulation. Once coagulation occurs, it causes the following technical problems: Severely distorted test results: Cell counts are significantly lower due to cell encapsulation by clots, and biochemical indicators (such as protein quantification) cannot reflect the true concentration due to protein precipitation, directly affecting the doctor's assessment of the degree of immune inflammatory response in the patient's central nervous system. Sample waste and repeated punctures: Coagulated samples cannot be used for subsequent flow cytometry or pathogen culture, necessitating a second lumbar puncture. This not only increases patient suffering and the risk of intracranial infection but also prolongs the diagnostic process. Therefore, designing a CSF collection device that can actively prevent CSF coagulation at the collection source has become a pressing technical challenge in this field. Summary of the Invention HK 30135845 A Specification 2 In order to improve the problem of unsatisfactory anti-coagulation effect, the present invention provides a lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammation.This invention provides a lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammatory conditions, employing the following technical solution: A lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammatory conditions includes a base. A rotating shaft is rotatably connected to the inner cavity of the base via bearings. A driving mechanism is provided on the surface of the rotating shaft. Support plates are fixedly connected to both sides of the top of the rotating shaft. A positioning frame is fixedly connected to the top of the two support plates on opposite sides. Collection bottles are evenly inserted into the inner surface of the positioning frame, and a sealing plug is provided on the top of each collection bottle. The driving mechanism includes an electric push rod, which is fixedly installed on the back of the inner cavity of the base. A push plate is fixedly connected to the telescopic end of the electric push rod. A toothed plate is fixedly connected to the front of the push plate. A gear is fixedly connected to the outer surface of the rotating shaft, and the outer surface of the toothed plate meshes with the outer surface of the gear. By adopting the above technical solution, the rotating shaft, positioning frame, and collection bottle can be driven to swing slightly. The liquid relies on inertia and gravity to form a wave-like flow or overall translation within the bottle, achieving gentle mixing with zero shear force and preventing cerebrospinal fluid coagulation. Optionally, a protective cover is fixedly connected to the top of the base, and handles are fixedly connected to both sides of the top of the protective cover. This technical solution protects the periphery of the collection bottle. Optionally, a partition is fixedly connected to the inner surface of the positioning frame, and the outer surface of the collection bottle is inserted into the inner surface of the partition. This technical solution limits the movement of the collection bottle. Optionally, a protective pad is fixedly connected to the bottom of the inner cavity of the positioning frame, and the top of the protective pad contacts the bottom of the collection bottle. This technical solution protects the bottom of the collection bottle. Optionally, a slider is fixedly connected to the bottom of the toothed plate, and a groove for cooperating with the slider is opened at the bottom of the inner cavity of the base. The outer surface of the slider is slidably connected to the inner surface of the groove. This technical solution guides and supports the movement of the toothed plate. Optionally, through slots are opened on both sides of the top of the base, and the outer surface of the support plate is slidably connected to the inner surface of the through slot. This technical solution facilitates the back-and-forth swinging of the support plate. Optionally, rubber pads with a thickness of 4mm are fixedly connected to each of the four corners of the base. This provides protection for the bottom of the base. Optionally, the inner surface of the collection bottle is coated with an MPC biomimetic coating, and the inside of the collection bottle is equipped with a heat-insulating layer. This prevents protein adhesion to the bottle wall and improves the heat retention effect on the cerebrospinal fluid.In summary, the present invention has the following beneficial effects: By setting up a collection bottle and a sealing plug, the present invention can collect and store cerebrospinal fluid obtained from lumbar punctures. The positioning frame facilitates the positioning of the collection bottle. The electric push rod, push plate, toothed plate, and gears drive the rotating shaft, positioning frame, and collection bottle to swing slightly. The liquid, relying on inertia and gravity, forms a wave-like flow or a uniform translation within the bottle, achieving gentle mixing with zero shear force and preventing cerebrospinal fluid coagulation. Figure 1 is a schematic diagram of the structure of the present invention; Figure 2 is a cross-sectional view of the structure of the present invention; Figure 3 is a right-side cross-sectional view of the base structure of the present invention; Figure 4 is a cross-sectional view of the collection bottle structure of the present invention; Figure 5 is a partial enlarged view of point A in Figure 4 of the present invention. HK 30135845 A Instruction Manual 4 Figure: 1. Base; 2. Rotating shaft; 3. Drive mechanism; 301. Electric push rod; 302. Push plate; 303. Toothed plate; 304. Gear; 4. Support plate; 5. Positioning frame; 6. Collection bottle; 7. Protective cover; 8. Handle; 9. Partition; 10. Protective pad; 11. Slider; 12. Slide groove; 13. Rubber pad; 14. Through groove; 15. Sealing plug; 16. MPC biomimetic coating; 17. Insulation layer. Detailed Description The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example: Referring to Figures 1-5, a lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammation includes a base 1. The inner cavity of the base 1 is rotatably connected to a rotating shaft 2 via bearings. A drive mechanism 3 is provided on the surface of the rotating shaft 2. Support plates 4 are fixedly connected to both sides of the top of the rotating shaft 2. Positioning frames 5 are fixedly connected to the top of the opposite side of the two support plates 4. Collection bottles 6 are evenly inserted into the inner surface of the positioning frames 5. A sealing plug 15 is provided on the top of the collection bottles 6. The drive mechanism 3 includes an electric push rod 301, which is fixedly installed on the back of the inner cavity of the base 1. A push plate 302 is fixedly connected to the telescopic end of the electric push rod 301. A toothed plate 303 is fixedly connected to the front of the push plate 302. A gear 304 is fixedly connected to the outer surface of the rotating shaft 2. The outer surface of the toothed plate 303 meshes with the outer surface of the gear 304. As a further technical optimization of the present invention, a protective cover 7 is fixedly connected to the top of the base 1, and handles 8 are fixedly connected to both sides of the top of the protective cover 7.As a further technical optimization of the present invention, a partition 9 is fixedly connected to the inner surface of the positioning frame 5, and the outer surface of the collection bottle 6 is inserted into the inner surface of the partition 9. As a further technical optimization of the present invention, a protective pad 10 is fixedly connected to the bottom of the inner cavity of the positioning frame 5, and the top of the protective pad 10 contacts the bottom of the collection bottle 6. As a further technical optimization of the present invention, a slider 11 is fixedly connected to the bottom of the toothed plate 303, and a groove 12 for use with the slider 11 is opened at the bottom of the inner cavity of the base 1, and the outer surface of the slider 11 is slidably connected to the inner surface of the groove 12. As a further technical optimization of the present invention, through grooves 14 are opened on both sides of the top of the base 1, and the outer surface of the support plate 4 is slidably connected to the inner surface of the through groove 14. As a further technical optimization of the present invention, rubber pads 13 are fixedly connected to the four corners of the bottom of the base 1, and the thickness of the rubber pads 13 is 4mm. As a further technical optimization of the present invention, the inner surface of the collection bottle 6 is provided with an MPC biomimetic coating 16, and the interior of the collection bottle 6 is provided with a heat insulation layer 17.In this embodiment: By setting up a collection bottle 6 and a sealing plug 15, lumbar puncture cerebrospinal fluid can be collected and stored. A positioning frame 5 facilitates the positioning of the collection bottle 6. An electric push rod 301, push plate 302, toothed plate 303, and gear 304 drive the rotating shaft 2, positioning frame 5, and collection bottle 6 to swing slightly. The liquid, relying on inertia and gravity, forms a wave-like flow or overall translation within the bottle. Since there are no high-speed rotating solid objects inside the liquid, the fluid shear force is almost zero, approaching 0 Pa, achieving gentle mixing with zero shear force and preventing cerebrospinal fluid coagulation. The left-right swaying has a certain biomimetic quality; by setting a specific frequency and tilt angle, it can simulate the swaying state of cerebrospinal fluid during human breathing or slight head movements. The tilt angle is adjustable from ±5° to ±15°, and the swaying frequency is 20-60 times / minute. The protective cover 7 installed on the top of the base 1 effectively isolates external dust and foreign objects from contaminating the transmission components and collection bottle 6. Handles 8 are provided on both sides. To facilitate handling and transfer by medical staff, the rubber pads 13 fixed at the four corners of the base 1 increase the friction between the device and the table, preventing slippage during operation and also providing shock absorption. The protective pads 10 and partitions 9 cushion the bottom of the bottle during movement or transport, effectively preventing breakage of the collection bottle 6 or sample spillage due to collisions, ensuring sample integrity and operational safety. The slider 11 and groove 12 ensure that the toothed plate 303, driven by the electric push rod 301, always moves precisely back and forth along a straight line, guaranteeing the meshing accuracy with the gear 304 and preventing jamming. This ensures the long-term stability and reliability of the drive mechanism 3. The insulation layer 17 inside the collection bottle 6 effectively slows down heat loss from the isolated cerebrospinal fluid, maintaining the activity of cells and proteins. Simultaneously, the MPC biomimetic coating 16 applied to the inner surface of the collection bottle 6... It mimics the structure of biological cell membranes, which can significantly inhibit the adsorption and denaturation of proteins and effectively prevent proteins and inflammatory cells in cerebrospinal fluid from coagulating, precipitating or adhering to the bottle wall.The working principle of this device is based on actively simulating physiological shaking and low shear force mixing, thereby inhibiting the coagulation process of cerebrospinal fluid after it leaves the body. The specific workflow is as follows: After obtaining cerebrospinal fluid through lumbar puncture, medical staff insert the end of the drainage tube through the sealing plug 15 into the collection bottle 6. The cerebrospinal fluid flows into the collection bottle 6 under the action of gravity. The MPC biomimetic coating 16 coated on the inner wall of the collection bottle 6 begins to function immediately upon contact, simulating the cell membrane structure and hindering the initial adsorption of proteins. This is the "first anti-coagulation barrier". At the same time, the heat insulation layer 17 inside the bottle wall slows down heat loss, maintains the sample temperature, and slows down the rate at which proteins precipitate due to cooling. After collection or during collection, the drive mechanism 3 is activated. The electric push rod 301 begins to perform regular telescopic movements, pushing the push plate 302 and the toothed plate 303 fixed to it to perform reciprocating linear movements in the horizontal direction. The toothed plate 303, through the teeth on its surface, precisely meshes with the gear 304 to drive it to rotate at small angles in both directions. The rotation of the shaft 2, which is fixed to it, rotates synchronously within the base 1. The oscillation of the shaft 2 is transmitted to the collection bottle 6 through the support plate 4 and the positioning frame 5, causing the collection bottle 6 to oscillate back and forth regularly according to preset parameters (such as tilt angle ±5° to ±15°, frequency 20-60 times / minute). During the oscillation of the collection bottle 6, the cerebrospinal fluid inside it forms a wave-like flow or overall translation within the bottle due to inertia and gravity. This motion mode is different from traditional magnetic stirrers or shakers. There are no high-speed rotating solid objects inside, so the fluid shear force generated on the protein molecules and inflammatory cells in the liquid is extremely low (close to 0 Pa). This gentle mixing with "zero shear force" can effectively prevent cell precipitation and aggregation and protein network formation (i.e., coagulation) through the macroscopic flow of the liquid, and will not damage cell morphology or cause protein denaturation due to high shear force, thus ensuring the accuracy of subsequent test results. HK 30135845 A Instruction Manual 7 During the entire operation, the slider 11 and the groove 12 The coordination of the components ensures the linearity of the movement and the smoothness of the meshing of the toothed plate 303; the protective cover 7 and the partition 9 ensure the stability of the collection bottle 6 during shaking, preventing it from falling out or colliding. In summary, this device, through the combination of the passive anti-adsorption of the MPC biomimetic coating 16 and the active anti-coagulation of low-frequency oscillation, constructs a mild, biomimetic environment for the preservation of cerebrospinal fluid in vitro, solving the technical problem of easy coagulation of cerebrospinal fluid from both physical and materials science perspectives. The above are all preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made according to the structure, shape, and principle of the present invention should be covered within the scope of protection of the present invention.HK 30135845 A Claim 1 1. A lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammation, comprising a base (1), characterized in that: the inner cavity of the base (1) is rotatably connected to a rotating shaft (2) via a bearing, the surface of the rotating shaft (2) is provided with a driving mechanism (3), both sides of the top of the rotating shaft (2) are fixedly connected to support plates (4), the top of the two support plates (4) on opposite sides is fixedly connected to a positioning frame (5), the inner surface of the positioning frame (5) is uniformly inserted with a collection bottle (6), the top of the collection bottle (6) is provided with a sealing plug (15); the driving mechanism (3) includes an electric push rod (301), the electric push rod (301) is fixedly installed on the back of the inner cavity of the base (1), the telescopic end of the electric push rod (301) is fixedly connected to a push plate (302), the front side of the push plate (302) is fixedly connected to a toothed plate (303), the rotating shaft (2) 1. A gear (304) is fixedly connected to the outer surface of the base (1), and the outer surface of the toothed plate (303) meshes with the outer surface of the gear (304). 2. A neuroinflammatory lumbar puncture cerebrospinal fluid collection device with anti-coagulation function according to claim 1, characterized in that: a protective cover (7) is fixedly connected to the top of the base (1), and handles (8) are fixedly connected to both sides of the top of the protective cover (7). 3. A neuroinflammatory lumbar puncture cerebrospinal fluid collection device with anti-coagulation function according to claim 1, characterized in that: a partition (9) is fixedly connected to the inner surface of the positioning frame (5), and the outer surface of the collection bottle (6) is inserted into the inner surface of the partition (9). 4. A neuroinflammatory lumbar puncture cerebrospinal fluid collection device with anti-coagulation function according to claim 1, characterized in that: a protective pad (10) is fixedly connected to the bottom of the inner cavity of the positioning frame (5), and the top of the protective pad (10) contacts the bottom of the collection bottle (6). 5. A lumbar puncture cerebrospinal fluid collection device with anticoagulation function for neuroinflammatory patients according to claim 1, characterized in that: a slider (11) is fixedly connected to the bottom of the toothed plate (303), and a groove (12) for use with the slider (11) is opened at the bottom of the inner cavity of the base (1), and the outer surface of the slider (11) is slidably connected to the inner surface of the groove (12). 6. A lumbar puncture cerebrospinal fluid collection device with anticoagulation function for neuroinflammatory patients according to claim 1, characterized in that: through grooves (14) are opened on both sides of the top of the base (1), and the outer surface of the support plate (4) is slidably connected to the inner surface of the through groove (14).7. A lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammatory patients according to claim 1, characterized in that: rubber pads (13) are fixedly connected to the four corners of the bottom of the base (1), and the thickness of the rubber pads (13) is 4mm. 8. A lumbar puncture cerebrospinal fluid collection device with anti-coagulation function for neuroinflammatory patients according to claim 1, characterized in that: an MPC biomimetic coating (16) is provided on the inner surface of the collection bottle (6), and a heat insulation layer (17) is provided inside the collection bottle (6). HK 30135845 A Instruction Manual Figure 1 Figure 1 HK 30135845 A Instruction Manual Figure 2 Figure 2 HK 30135845 A Instruction Manual Figure 3 Figure 3 HK 30135845 A Instruction Manual Figure 4 Figure 4 HK 30135845 A Instruction Manual Figure 5 Figure 5 HK 30135845 A.

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