A disinfectant and disinfectant wipes and their applications

Abstract

The invention relates to the technical field of disinfection, in particular to a disinfectant, a disinfection wet tissue and application. The disinfectant provided by the invention comprises the following components: nano-silver, composite quaternary ammonium salt and matrine extracting solution, and the composite quaternary ammonium salt is a mixture of single-chain quaternary ammonium salt and double-chain quaternary ammonium salt. The disinfectant provided by the invention is composed of the nano-silver, the matrine extracting solution and the composite quaternary ammonium salt formed by mixing the single-chain quaternary ammonium salt and the double-chain quaternary ammonium salt, and the invention further provides the disinfectant wet tissue prepared by using the disinfectant, so that the disinfectant wet tissue can efficiently kill AIDS viruses, influenza A viruses, bacteria and fungi, is lasting in antifungal, bacterial and virus resistance, and is free of toxic and side effects. The ointment is non-irritant to mucous membranes, safe and lasting.

Description

(19) State Intellectual Property Office (12) Invention Patent Application (10) Application Publication Number (43) Application Publication Date (21) Application Number 202511797944.0 (22) Application Date 2025.12.01 (71) Applicant Haitao Biotechnology (Zhejiang) Co., Ltd. Address 314001, Room 1107 & 1109, Building 8, Phase II, Chinese Academy of Sciences, No. 778, Yatai Road, Daqiao Town, Nanhu District, Jiaxing City, Zhejiang Province (72) Inventor Sun Haitao (74) Patent Agency Beijing Sanju Sunshine Intellectual Property Agency Co., Ltd. 11250 Patent Attorney Wang Jingjing (51) Int.Cl. A61K 33 / 38 (2006.01) A61K 31 / 4375 (2006.01) A61K 31 / 14 (2006.01) A61K 31 / 045 (2006.01) A61K 8 / 19(2006.01) A61K 8 / 41(2006.01) A61K 8 / 49(2006.01) A61K 8 / 34(2006.01) A61K 8 / 02(2006.01) A61Q 17 / 00(2006.01) A61P 31 / 18(2006.01) A61P 31 / 16(2006.01) A61P 31 / 04(2006.01) A61P 31 / 10(2006.01) A01N 59 / 16(2006.01) A01N 43 / 90(2006.01) A01N 33 / 12(2006.01) A01N 25 / 34(2006.01) A01P 1 / 00(2006.01) (54) Invention Title: A Disinfectant and Disinfectant Wipes and Their Applications (57) Abstract: This invention relates to the field of disinfection technology, specifically to a disinfectant and disinfectant wipes and their applications. The disinfectant provided by this invention comprises the following components: nano-silver, a complex quaternary ammonium salt, and matrine extract, wherein the complex quaternary ammonium salt is a mixture of single-chain and double-chain quaternary ammonium salts. The disinfectant provided by this invention is composed of nano-silver, matrine extract, and a complex quaternary ammonium salt formed by mixing single-chain and double-chain quaternary ammonium salts. This invention also provides disinfectant wipes made using this disinfectant, which can effectively kill HIV, influenza A virus, bacteria, and fungi, providing long-lasting antifungal, antibacterial, and antiviral effects, without irritating mucous membranes, and are safe and durable. Claims (1 page), Description (13 pages), CN 121971489 A 2026.05.05 CN 1 21 97 14 89 A 1. A disinfectant, characterized in that it comprises the following components: nano-silver, a complex quaternary ammonium salt, and matrine extract; wherein the complex quaternary ammonium salt is a mixture of single-chain and double-chain quaternary ammonium salts. 2.According to claim 1, the disinfectant is characterized in that the mass ratio of single-chain quaternary ammonium salt to double-chain quaternary ammonium salt in the composite quaternary ammonium salt is 1.0:0.5-2, preferably 1.0:0.8-1.4; the matrine extract is extracted by ethanol extraction. 3. According to claim 1, the disinfectant contains 5-10 mg / L of nano-silver, 0.005-0.02 wt% of single-chain quaternary ammonium salt, 0.005-0.02 wt% of double-chain quaternary ammonium salt, and 3-5 vol% of matrine extract. 4. According to claim 1, the disinfectant contains at least one of dodecyl dimethyl benzyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, hexadecyl trimethyl ammonium chloride, and hexadecyl trimethyl ammonium bromide; the double-chain quaternary ammonium salt contains at least one of dialcyl dimethyl ammonium chloride, dialcyl dimethyl ammonium bromide, dioctyl dimethyl ammonium chloride, and dioctyl dimethyl ammonium bromide. 5. The disinfectant according to claim 1, characterized in that the disinfectant further comprises ethanol, a surfactant, and a solubilizer. 6. The disinfectant according to claim 5, characterized in that the surfactant comprises at least one of polysorbate 80 or polyoxyethylene castor oil; the solubilizer comprises at least one of ethylene glycol or propylene glycol. 7. The disinfectant according to claim 5, characterized in that, based on the total weight of the disinfectant, the ethanol content is 11.25-15 v / v%, the surfactant content is 1-2 v / v%, and the solubilizer content is 0.5-1 v / v%. 8. A disinfectant wipe, characterized in that it comprises the disinfectant according to any one of claims 1-7. 9. The disinfectant wipe according to claim 8, characterized in that the ratio of the disinfectant to the nonwoven fabric substrate is 3.0-3.5:1, in mL:g; the nonwoven fabric substrate comprises viscose and polyester, and based on the total weight of the nonwoven fabric substrate, the viscose content is 40-45 wt%, and the polyester content is 55-60 wt%. 10.The application of a disinfectant according to any one of claims 1-7 or a disinfectant wipe according to any one of claims 8-9 in the treatment of HIV, influenza A virus, bacteria, and fungi. Claims 1 / 1 page 2 CN 121971489 A A disinfectant and disinfectant wipes and their application technical field

[0001] This invention relates to the field of disinfection technology, specifically to a disinfectant and disinfectant wipes and their application. Background Art

[0002] With the emergence of new pathogens and drug-resistant bacteria, scientific and precise disinfection strategies are becoming increasingly important. Traditional disinfectants, while effective against bacteria and some viruses, lack durability and safety. Among them, AIDS (Acquired Immunodeficiency Syndrome) is a serious infectious disease caused by the human immunodeficiency virus (HIV), which is mainly transmitted through blood, sexual contact, and mother-to-child transmission. Preventing the spread of HIV is crucial in the fields of medical care, nursing, and personal hygiene. Because HIV has extremely high infectivity and latency, its outer envelope protein can specifically bind to host cells, thereby invading the immune system. Therefore, effective disinfection measures are of great significance in blocking the virus transmission chain. Currently, to inhibit the spread of HIV, most disinfectants are prepared by mixing low-concentration sodium hypochlorite solution with alcohol. Sodium hypochlorite destroys the viral protein structure through oxidation, while alcohol dissolves the viral lipid envelope, rendering it ineffective. However, this traditional disinfectant has limited inactivation effect on HIV, especially in the presence of organic matter (such as blood or bodily fluids), where disinfection efficiency is further reduced. Furthermore, HIV may form biofilms under certain conditions, enhancing its resistance to external disinfectants, making it difficult for conventional disinfection methods to completely inactivate the virus.

[0003] To improve disinfection effectiveness, existing technologies add halogenated substances such as chlorine or iodine to disinfectants, utilizing their strong oxidizing properties to enhance the killing ability against HIV. However, these substances are highly corrosive and may damage medical devices, skin, and mucous membranes; long-term use can also lead to environmental pollution. Simultaneously, while these disinfectants can reduce viral load in a short time, their action time is short and they cannot provide lasting protection.

[0004] Therefore, the technical problem to be solved by the present invention is to overcome the defects of poor safety and durability of disinfectants in the prior art, thereby providing a disinfectant and disinfectant wipes and their application.

[0005] To this end, the present invention provides the following technical solution: A disinfectant, comprising the following components: nano-silver, a compound quaternary ammonium salt, and matrine extract.

[0006] The compound quaternary ammonium salt is a mixture of single-chain quaternary ammonium salt and double-chain quaternary ammonium salt.

[0007] The mass ratio of single-chain quaternary ammonium salt to double-chain quaternary ammonium salt in the compound quaternary ammonium salt is 1.0:0.5-2, preferably 1.0:0.8-1.4.

[0008] The matrine extract is extracted using an ethanol extraction method.

[0009] The content of the nano-silver is 5-10 mg / L, the content of the single-chain quaternary ammonium salt is 0.005-0.02 wt%, the content of the double-chain quaternary ammonium salt is 0.005-0.02 wt%, and the content of the matrine extract is 3-5 vol%.

[0010] The single-chain quaternary ammonium salt includes at least one of dodecyl dimethyl benzyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, hexadecyl trimethyl ammonium chloride, and hexadecyl trimethyl ammonium bromide.

[0011] The double-chain quaternary ammonium salt includes at least one of dialcyl dimethyl ammonium chloride, dialcyl dimethyl ammonium bromide, dioctyl dimethyl ammonium chloride, and dioctyl dimethyl ammonium bromide.

[0012] The disinfectant also includes ethanol, a surfactant, and a solubilizer. Instruction Manual 1 / 13 Page 3 CN 121971489 A

[0013] The surfactant includes at least one of polysorbate 80 or polyoxyethylene castor oil.

[0014] The solubilizer includes at least one of ethylene glycol or propylene glycol.

[0015] Based on the total weight of the disinfectant, the ethanol content is 11.25-15 v / v%, the surfactant content is 1-2 v / v%, and the solubilizer content is 0.5-1 v / v%.

[0016] The present invention also provides a disinfectant wipe, comprising the above-mentioned disinfectant.

[0017] The ratio of the disinfectant to the nonwoven fabric substrate is 3.0-3.5:1, in mL:g. The nonwoven fabric substrate comprises viscose and polyester, and based on the total weight of the nonwoven fabric substrate, the viscose content is 40-45 wt%, and the polyester content is 55-60 wt%.

[0018] The present invention also provides the application of the above-mentioned disinfectant or the above-mentioned disinfectant wipes in anti-HIV, anti-H1N1 influenza virus, antibacterial and antifungal activities.

[0019] The technical solution of the present invention has the following advantages: 1.The present invention provides a disinfectant comprising the following components: nano-silver, a complex quaternary ammonium salt, and matrine extract, wherein the complex quaternary ammonium salt is a mixture of single-chain and double-chain quaternary ammonium salts. The disinfectant provided by the present invention, composed of nano-silver, matrine extract, and a complex quaternary ammonium salt formed by mixing single-chain and double-chain quaternary ammonium salts, can effectively kill HIV, influenza A virus, bacteria, and fungi. It exhibits long-lasting antifungal, antibacterial, and antiviral activity, is non-irritating to mucous membranes, and is safe and durable.

[0020] Nano-silver has broad-spectrum antibacterial properties, capable of killing various bacteria, fungi, and viruses, including drug-resistant strains. The silver ions (Ag+) released by nano-silver can directly destroy viruses, exhibiting strong antiviral capabilities. Simultaneously, it can penetrate cell membranes and bind to various biomolecules within bacterial cells, interfering with key physiological processes such as DNA replication, protein synthesis, and signal transduction, thereby killing bacteria and inhibiting bacterial reproduction. The combined use of compound quaternary ammonium salt and nano silver not only improves the disinfection effect, efficiency, and durability, but also broadens the bactericidal spectrum, reduces the amount of nano silver used, and improves safety. Furthermore, the combination of single-chain and double-chain quaternary ammonium salts has a synergistic effect, maintaining low toxicity, low irritation, and non-corrosive safety characteristics even at reduced concentrations. Moreover, this invention utilizes matrine extract to further enhance the bactericidal effect while reducing the amount of quaternary ammonium salt used, thus reducing skin irritation.

[0021] 2. The disinfectant provided by this invention also includes ethanol, surfactant, and solubilizer. This invention utilizes solubilizer to synergistically enhance the antibacterial and antiviral effects of nano silver, compound quaternary ammonium salt, matrine extract, and ethanol. The surfactant reduces surface tension, increases contact with skin or object surfaces, and enhances the disinfection effect of the disinfectant components.

[0022] 3.This invention provides a disinfectant wipe, comprising the aforementioned disinfectant solution. The disinfectant wipe provided by this invention can be used for cleaning and caring for the local skin or mucous membranes of patients with AIDS, influenza A virus, etc., to help alleviate related symptoms. The disinfectant wipe is easy to carry and use, and is suitable for various scenarios, including medical settings, home protection, and self-protection for high-risk groups. Detailed Embodiments

[0023] The following embodiments are provided to better understand this invention and are not limited to the preferred embodiments described. They do not constitute a limitation on the content and scope of protection of this invention. Any product identical or similar to this invention derived by anyone under the guidance of this invention or by combining features of this invention with other prior art falls within the scope of protection of this invention.

[0024] Where specific experimental steps or conditions are not specified in the embodiments, they can be performed according to the conventional experimental steps or conditions described in the literature in this field. Reagents or instruments whose manufacturers are not specified are all commercially available conventional reagent products. Instruction manual 2 / 13 page 4 CN 121971489 A

[0025] Example 1 This example provides a method for preparing an anti-HIV disinfectant, the specific steps and parameters are as follows: (1) Take 1.7g of silver nitrate (purity 99.9wt%, Jinan Leqi Chemical Co., Ltd.) and grind the silver nitrate into powder of more than 200 mesh in an agate mortar. Add the ground powder to a three-necked flask containing 108mL of anhydrous ethanol, heat under reflux for 2h, and after cooling, obtain a 1.0wt% (10000mg / L) nano-silver anhydrous ethanol solution. Take 100mL of this solution and dilute it with anhydrous ethanol to 1000mL to obtain a 1000mg / L nano-silver solution with an average nano-silver particle size of 20nm, for later use.

[0026] (2) Take 100 mL of dodecyl dimethyl benzyl ammonium chloride (40 wt% aqueous solution of single-chain quaternary ammonium salt 1227, purchased from Zaozhuang Fengsen Water Treatment Technology Co., Ltd.) and add it to a 500 mL beaker. Stir at 150 rpm and add 57 mL of didecyl dimethyl ammonium chloride (70 wt% aqueous solution of double-chain quaternary ammonium salt DDAC, purchased from Wanyin (Hebei) New Material Technology Co., Ltd.). Then dilute with pure water to 200 mL to obtain a composite quaternary ammonium salt solution with a single-chain quaternary ammonium salt concentration of 20 wt% and a double-chain quaternary ammonium salt concentration of 20 wt%, for later use.

[0027] (3) Wash, dry, and crush the Sophora flavescens. Pass it through a 40-mesh sieve. Take 20 g of the sieved Sophora flavescens powder and add it to a 250 mL three-necked flask containing 100 mL of 60 vol% ethanol solution. Reflux and extract twice, 2 h each time. After extraction, filter while hot. Combine the filtrates, add pure water to the filtrate, bringing the total volume to 200 mL, to obtain matrine extract, which is then set aside. Referring to the regulations for the determination of matrine content in Part I of the 2020 edition of the Chinese Pharmacopoeia, the total matrine content in the matrine extract was determined to be 1%.23wt%, of which matrine content is 0.85wt% and oxymatrine content is 0.38wt%.

[0028] (4) Add 0.5mL of nano silver solution prepared in step (1), 0.1mL of composite quaternary ammonium salt solution prepared in step (2), 3mL of matrine extract prepared in step (3), and 15mL of 75vol% ethanol to a beaker in sequence, then add 1.0mL of polysorbate 80 and 0.5mL of ethylene glycol, stir at 150rpm for 5min to mix, then dilute with pure water to 100mL, and continue stirring for 2min to obtain anti-HIV disinfectant.

[0029] Example 2 This example provides a method for preparing an anti-HIV disinfectant, the specific steps and parameters are as follows: 1 mL of the nano silver solution prepared in step (1) of Example 1, 0.05 mL of the composite quaternary ammonium salt solution prepared in step (2) of Example 1, 5 mL of the matrine extract prepared in step (3) of Example 1, and 20 mL of 75 vol% ethanol are added to a beaker in sequence, and then 2 mL of polyoxyethylene castor oil and 1 mL of ethylene glycol are added to it. The mixture is stirred at 150 rpm and then diluted with pure water to 100 mL. The mixture is stirred for 2 min to obtain the anti-HIV disinfectant.

[0030] Example 3 This example provides a method for preparing an anti-HIV disinfectant, the specific steps and parameters are as follows: 0.75 mL of the nano silver solution prepared in step (1) of Example 1, 0.05 mL of the composite quaternary ammonium salt solution prepared in step (2) of Example 1, 4 mL of the matrine extract prepared in step (3) of Example 1, and 17.5 mL of 75 vol% ethanol are added to a beaker in sequence, and then 1.5 mL of polyoxyethylene castor oil and 0.75 mL of propylene glycol was stirred and mixed at 150 rpm, then diluted with pure water to 100 mL, and stirred for 2 min to obtain an anti-HIV disinfectant solution.

[0031] Example 4 The difference between this example and Example 1 is that in step (2), dodecyl dimethyl benzyl ammonium chloride was replaced by dodecyl dimethyl benzyl ammonium bromide; and didecyl dimethyl ammonium chloride was replaced by didecyl dimethyl ammonium bromide. Finally, a composite quaternary ammonium salt solution with a single-chain quaternary ammonium salt concentration of 20 wt% and a double-chain quaternary ammonium salt concentration of 20 wt% was obtained.

[0032] Example 5 The difference between this example and Example 1 is that in step (2), dodecyl dimethyl benzyl ammonium chloride was replaced by hexadecyl trimethyl ammonium chloride; and didecyl dimethyl ammonium chloride was replaced by dioctyl dimethyl ammonium chloride. The final product is a composite quaternary ammonium salt solution with a single-chain quaternary ammonium salt concentration of 20 wt% and a double-chain quaternary ammonium salt concentration of 20 wt%.

[0033] Example 6 The difference between this example and Example 1 is that in step (2), the dodecyl dimethyl benzyl ammonium chloride is replaced by hexadecyl trimethyl ammonium bromide; the didecyl dimethyl ammonium chloride is replaced by dioctyl dimethyl ammonium bromide. The final product is a composite quaternary ammonium salt solution with a single-chain quaternary ammonium salt concentration of 20 wt% and a double-chain quaternary ammonium salt concentration of 20 wt%.

[0034] Example 7 The difference between this example and Example 1 is that in step (2), the dodecyl dimethyl benzyl ammonium chloride is replaced by hexadecyl trimethyl ammonium bromide; the didecyl dimethyl ammonium chloride is replaced by dioctyl dimethyl ammonium chloride. The final product is a composite quaternary ammonium salt solution with a single-chain quaternary ammonium salt concentration of 20 wt% and a double-chain quaternary ammonium salt concentration of 20 wt%.

[0035] Example 8 The difference between this example and Example 1 is that in step (2), the dodecyl dimethyl benzyl ammonium chloride is replaced by hexadecyl trimethyl ammonium chloride. Finally, a composite quaternary ammonium salt solution with a single-chain quaternary ammonium salt concentration of 20wt% and a double-chain quaternary ammonium salt concentration of 20wt% is obtained.

[0036] Example 9 The difference between this example and Example 1 is that in step (4), ethylene glycol is replaced by propylene glycol.

[0037] Example 10 The difference between this example and Example 1 is that in step (4), polysorbate 80 is replaced by polyoxyethylene castor oil.

[0038] Comparative Example 1 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1. The difference is that step (4) does not contain a composite quaternary ammonium salt solution, and an equal volume of nano-silver is used to replace the composite quaternary ammonium salt solution in step (4). That is, 0.6 mL of the nano-silver solution prepared in step (1) of Example 1, 3 mL of the matrine extract prepared in step (3) of Example 1, and 15 mL of 75 vol% ethanol were added to a beaker in sequence. Then, 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol were added to the beaker and stirred at 150 rpm. The mixture was then diluted with pure water to 100 mL and stirred evenly at 150 rpm to obtain an anti-HIV disinfectant.

[0039] Comparative Example 2 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1. The difference is that step (4) does not contain matrine extract, and an equal volume of pure water is used to replace the matrine extract in step (4). That is, 0.5 mL of the nano silver solution prepared in step (1) of Example 1, 0.1 mL of the composite quaternary ammonium salt solution prepared in step (2) of Example 1, and 15 mL of 75 vol% ethanol were added to a beaker in sequence, and then 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol were added to it. The mixture was stirred at 150 rpm and then diluted with pure water to 100 mL. The mixture was stirred evenly at 150 rpm to obtain an anti-HIV disinfectant.

[0040] Comparative Example 3 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as those in Example 1. The difference is that it does not contain solubilizers and surfactants. An equal volume of pure water is used to replace the solubilizers and surfactants in step (4). That is, 0.5 mL of the nano silver solution prepared in step (1) of Example 1, 0.1 mL of the composite quaternary ammonium salt solution prepared in step (2) of Example 1, 3 mL of the matrine extract prepared in step (3) of Example 1, and 15 mL of 75 vol% ethanol were added to a beaker in sequence, stirred and mixed at 150 rpm, and then diluted with pure water to 100 mL. The mixture was stirred evenly at 150 rpm to obtain an anti-HIV disinfectant. Instruction manual 4 / 13 pages 6 CN 121971489 A

[0041] Comparative Example 4 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as those in Example 1. The difference is that the disinfectant does not contain the composite quaternary ammonium salt and the matrine extract. The composite quaternary ammonium salt solution in step (4) is replaced with an equal volume of nano silver, and the matrine extract in step (4) is replaced with an equal volume of pure water. That is, 0.6 mL of the nano silver solution prepared in step (1) and 15 mL of 75 vol% ethanol are added to a beaker in sequence, and then 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol was stirred and mixed at 150 rpm, then diluted with pure water to 100 mL, and stirred evenly at 150 rpm to obtain an anti-HIV disinfectant.

[0042] Comparative Example 5 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1. The difference is that the disinfectant does not contain nano-silver and matrine extract. An equal volume of composite quaternary ammonium salt solution is used to replace the nano-silver in step (4), and an equal volume of pure water is used to replace the matrine extract in step (4). That is, 0.6 mL of the composite quaternary ammonium salt solution prepared in step (2) and 15 mL of 75 vol% ethanol are added to a beaker in sequence, and then 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol are added to it. The mixture is stirred and mixed at 50 rpm, then diluted with pure water to 100 mL, and stirred evenly at 150 rpm to obtain an anti-HIV disinfectant.

[0043] Comparative Example 6 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1. The difference is that the disinfectant does not contain compound quaternary ammonium salt and nano silver. An equal volume of matrine extract is used to replace the nano silver and compound quaternary ammonium salt in step (4). That is, 3.6 mL of matrine extract prepared in step (3) and 15 mL of 75 vol% ethanol are added to a beaker in sequence. Then, 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol are added to the beaker. The mixture is stirred at 150 rpm and then diluted with pure water to 100 mL. The mixture is stirred evenly at 150 rpm to obtain the anti-HIV disinfectant.

[0044] Comparative Example 7 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1. The difference is that an equal volume of compound quaternary ammonium salt solution is used to replace the nano silver solution in step (1). That is, 0.6 mL of the compound quaternary ammonium salt solution prepared in step (2), 3 mL of the matrine extract prepared in step (3) of Example 1, and 15 mL of 75 vol% ethanol were added to a beaker in sequence, and then 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol were added to it. The mixture was stirred at 150 rpm and then diluted with pure water to 100 mL. The mixture was stirred evenly at 150 rpm to obtain an anti-HIV disinfectant.

[0045] Comparative Example 8 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1. The difference is that an equal volume of matrine extract is used instead of the nano silver solution in step (1). That is, 0.1 mL of the compound quaternary ammonium salt solution prepared in step (2), 3.5 mL of the matrine extract prepared in step (3) of Example 1, and 15 mL of 75 vol% ethanol were added to a beaker in sequence, and then 1 mL of polysorbate 80 and 0.5 mL of ethylene glycol were added to it.5 mL of ethylene glycol was stirred and mixed at 150 rpm, then diluted with pure water to 100 mL, and stirred evenly at 150 rpm to obtain an anti-HIV disinfectant.

[0046] Comparative Example 9 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1, except that an equal mass of dodecyl dimethyl benzyl ammonium chloride solution is used instead of dimethyl dialcyl ammonium chloride, and the concentration of the dodecyl dimethyl benzyl ammonium chloride solution is 40 wt%.

[0047] Comparative Example 10 This comparative example provides a method for preparing an anti-HIV disinfectant. The specific steps and parameters are the same as in Example 1, except that an equal mass of dimethyl dialcyl ammonium chloride solution is used instead of dodecyl dimethyl benzyl ammonium chloride, and the concentration of the dimethyl dialcyl ammonium chloride solution is 40 wt%.

[0048] Application Examples 1-20: Take 10 pieces of 150mm×200mm, 60g / m2 flat cross-woven nonwoven fabric substrate (the substrate contains 40wt% viscose and 60wt% polyester, with a total mass of 17.1g), fold them into shape, place them in an aluminized film bag, add 60.9mL of anti-HIV disinfectant solution to the aluminized film bag, seal it, and obtain anti-HIV disinfectant wipes.

[0049] Application Examples 1-20 correspond to the anti-HIV disinfectant wipes prepared in Examples 1-10 and Comparative Examples 1-10, respectively.

[0050] Test Example 1: HIV inactivation test was commissioned to Ingel Testing Technology Service (Shanghai) Co., Ltd., and the HIV inactivation effect of the disinfectant wipes was tested with reference to 2.1.1.10 of the "Disinfection Technical Specifications" (Ministry of Health, 2002 edition). The test results are shown in Table 1.

[0051] The detection method is as follows: 1. Take a viral suspension of the test strain (HIV-1 human immunodeficiency virus - AIDS virus) with a titer of 107-108 TCID50 / mL and set aside; 2. Take the anti-HIV disinfectant wipe squeeze liquid and the viral suspension from Application Examples 1-20 and place them in 25℃ for 30 min to equilibrate. Then add 0.2 mL of the viral suspension to 0.8 mL of the anti-HIV disinfectant wipe squeeze liquid. After acting for 5 min, immediately take out 0.1 mL and add 0.The reaction was terminated with 9 mL of neutralizing agent (the neutralizing agent was a PBS solution containing 5 wt% sodium thiosulfate, 1 wt% lecithin, 9 wt% Tween 80, and 2 wt% histidine) to obtain the virus disinfectant solution, which was used as the experimental group; 3. An equal volume of deionized water was used to replace the virus disinfectant solution in step 2 of this test example as the negative control group; 4. Each experimental group was placed in a 37℃, 5% CO2 incubator and cultured for 5 days. The virus titer was determined by the endpoint dilution method, and the experiment was repeated three times; 5. The average log value of HIV inactivation was calculated as: log value of virus infection titer in the negative control group - log value of virus infection titer in the experimental group.

[0052] Test Example 2 The inactivation of influenza A virus was commissioned to Zhongguancun International Medical Testing and Certification Technology Co., Ltd. The inactivation effect of disinfectant wipes on influenza A virus was tested with reference to 2.1.1.10 of the "Disinfection Technical Specifications" (Ministry of Health, 2002 edition). The test results are shown in Table 1.

[0053] The detection method is as follows: 1. The test uses a suspension of influenza virus H1N1 with a hemagglutination (HA) titer ≥1:640 TCID50 / mL, diluted with 3% bovine serum albumin organic interference substance, and kept at 20℃ for later use; 2. The test uses anti-HIV disinfectant wipe squeeze liquid. Take 0.1mL of the virus prepared in step 1 and add it to 0.4mL of disinfectant wipe squeeze liquid, mix, and let it act for 5.0min. Immediately take 0.1mL of the mixture and transfer it to 0.9mL of D / E neutralizing broth. Shake well to stop the disinfection. Use physiological saline to make serial dilutions, i.e., 10, 100, and 1000 times dilution. Take 0.2mL of the sample solution from each dilution and inoculate it into the allantoic cavity of 4 9-11 day old SPF chicken embryos. Collect the allantoic fluid. HA negative result indicates no infectious virus. The test temperature was constant at 20℃, and the test was repeated 3 times; 3. An equal volume of deionized water was used to replace the anti-HIV disinfectant wipe squeeze liquid in step 2 of this test example as a control group; 4. The titer of influenza A virus in the experimental group and the negative control group (unit: TCID50 / mL) was detected, and the average log value of inactivation of influenza A virus was calculated. The average log value of inactivation of influenza A virus = log value of virus infection titer in the negative control group - log value of virus infection titer in the experimental group.

[0054] Test Example 3 The bactericidal test refers to 2.1.1 of the "Disinfection Technical Specifications" (Ministry of Health, 2002 edition).7. The bactericidal effect of the disinfectant wipes was tested. The test results are shown in Table 1. (Instructions for use, page 6 / 13, CN 121971489 A)

[0055] The detection method is as follows: 1. Select the following bacterial species as test species: Staphylococcus aureus ATCC 6538, Escherichia coli 8099, and Candida albicans ATCC 10231. Adjust the bacterial suspension concentration to 1×10⁸ CFU / mL-5×10⁸ CFU / mL and the fungal suspension concentration to 1×10⁷ CFU / mL-5×10⁷ CFU / mL; 2. Use the anti-HIV disinfectant wipes squeezed out of Application Examples 1-20 as samples; 3. Take 0.5 mL of each test bacterial suspension and put it into a sterile test tube, then add 0.5 mL of 3% bovine serum albumin as an organic interfering agent, mix well, and keep at 20℃ for 5 min. Then, take 4.0 mL of the above anti-HIV disinfectant wipes squeezed out of Application Examples 1-20 and put it into each test tube and mix well; 4. After each test tube is incubated at 20℃ for 5 min, take 0.5 mL of the above mixture and add it to the test tube.5. Mix 5 mL of neutralizing agent (the neutralizing agent is a PBS solution containing 5 wt% sodium thiosulfate, 1 wt% lecithin, 9 wt% Tween 80 and 2 wt% histidine) to obtain a neutralized solution; 6. After neutralizing the solution at 20℃ for 10 min, take 1 mL of the neutralized solution and dilute it to three dilutions, namely 10, 100, and 1000 times. Incubate the diluted solution overnight at 37℃ on agar plates and count the bacteria; 7. Use PBS instead of the anti-HIV disinfectant wipes squeezed-out liquid as a positive control group for the bacterial bactericidal effect test, and use standard hard water (hardness of 342 mg / L, calculated as CaCO3) instead of the anti-HIV disinfectant wipes squeezed-out liquid as a positive control group for the fungal bactericidal effect test. Repeat steps 3-5; 8. The bactericidal rate of the anti-HIV disinfectant wipes = (number of colonies in the positive control group - number of colonies in the experimental group) / number of colonies in the positive control group × 100%.

[0056] Table 1 Average inactivation value and average bactericidal rate of anti-HIV disinfectant wipes (action time 5 min) Instruction manual 7 / 13 page 9 CN 121971489 A

[0057] According to Table 1, compared with Examples 1-3, the removal of the compound quaternary ammonium salt in Comparative Example 1 resulted in a significant decrease in the inactivation and bactericidal properties of the disinfectant wipes, decreasing by two orders of magnitude. This indicates that the compound quaternary ammonium salt has a strong synergistic effect on nano-silver, and the removal of the compound quaternary ammonium salt resulted in a poorer performance of the disinfectant's antiviral and bactericidal functions. The removal of matrine extract in Comparative Example 2 also resulted in a significant decrease in the inactivation and bactericidal properties of the disinfectant, indicating that the matrine extract in the disinfectant had a significant effect on inactivating viruses and killing bacteria. In Comparative Example 3, the removal of solubilizers and surfactants resulted in a significant decrease in both the virus inactivation value and bactericidal activity of the disinfectant. This indicates that the use of solubilizers and surfactants ensures that the disinfectant containing nano-silver solution, compound quaternary ammonium salt, and matrine extract can better perform its disinfection and sterilization functions. Comparative Examples 4-6, containing only nano-silver, compound quaternary ammonium salt, and matrine extract, showed weakened virus inactivation capabilities against HIV and influenza A virus, and very low bactericidal rates against Escherichia coli, Staphylococcus aureus, and Candida albicans, failing to meet the technical specifications for virus inactivation (virus inactivation value greater than 2) and bactericidal activity (bactericidal rate greater than 99.9%). Comparative Examples 7-10, with component substitutions, showed weakened virus inactivation capabilities against HIV and influenza A virus, and reduced bactericidal rates against Escherichia coli, Staphylococcus aureus, and Candida albicans, failing to meet the technical specifications for bactericidal activity (bactericidal rate greater than 99.9%).

[0058] Test Example 4: Stability test was conducted in accordance with the Disinfection Technical Specifications (2002 Edition) 2.2.3.1 and 2.2.3.2.1. The disinfectant wipes samples prepared in Examples 1-10 and Comparative Examples 9 and 10 were placed in an environment at 37°C for 90 days. The appearance and packaging were then observed, and the antiviral and antibacterial properties of the disinfectant wipes were tested according to the methods in Test Examples 1-3. These test conditions are equivalent to test conditions with a shelf life of two years.

[0059] Table 2 Average inactivation value and average bactericidal rate of anti-HIV disinfectant wipes (after 90 days of storage at 37℃, action time 5 min) Instruction manual 8 / 13 pages 10 CN 121971489 A

[0060] According to Table 2, compared with the anti-HIV disinfectant solution and disinfectant wipes prepared in Examples 1-10 of Table 1, after 90 days of storage at 37℃, the inactivation of viruses and bactericidal properties of the disinfectant wipes decreased slightly, by less than one order of magnitude, while maintaining an antiviral and antibacterial rate of over 99.9%, indicating that the disinfectant solution of nano-silver, compound quaternary ammonium salt, and matrine has storage stability. The anti-HIV wipes prepared in Comparative Examples 9 and 10, after 90 days of storage at 37℃, showed a slight decrease in the inactivation of viruses and bactericidal properties, failing to reach an antiviral and antibacterial rate of over 99.9%.

[0061] Test Example 5 Durability Test The sustained antibacterial test of the antiviral disinfectant wipes obtained in Examples 1-10 and Comparative Examples 9 and 10 was conducted according to the sustained antibacterial test method 5.2.7 of WS / T 650-2019 "Evaluation Method for Antibacterial and Bacteriostatic Effects". The test results are shown in Tables 3 and 4. The test method is as follows: 1. Reagents, culture medium and equipment Test strains: Staphylococcus aureus (ATCC6538), Escherichia coli (8099) Carrier: Polyethylene plastic sheet Reagents: Diluent (0.03 mol / L PBS containing 0.01% Tween 80), neutralizing agent (neutralizing agent is PBS solution containing 5 wt% sodium thiosulfate, 1 wt% lecithin, 9 wt% Tween 80 and 2 wt% histidine), nutrient agar, malt extract agar.

[0062] 2. Experimental Steps 2.1 Preparation of Long-Lasting Disinfectant Samples: Cut polyethylene plastic sheets into 50 mm × 50 mm pieces to make carrier samples. Apply the antiviral disinfectant wipes obtained in Examples 1-10 and Comparative Examples 9 and 10 to the surface of the sample three times, dry at room temperature, and use as the experimental group; untreated sample (sterilized by pressure steam) serves as the control group; both groups of sample samples are stored in the laboratory at room temperature.

[0063] 2.2 Long-Lasting Antibacterial Experimental Steps: 24-hour freshly cultured bacterial suspension is applied to the carrier. After 5 minutes of treatment, the carrier in the experimental group is added to the neutralizing agent, mixed, diluted, and inoculated; at the same time, parallel experiments are conducted using the control group sample. After 5 minutes of treatment, the carrier in the control group is added to the diluent, mixed, diluted, and inoculated. The bacterial vegetative cells are cultured at 36℃±1℃ for 48 hours, and the recovered bacterial count after carrier treatment in the control group is 1.0×10⁴ CFU / piece to 9.0×104 CFU / slice. First, test the bactericidal rates of two groups of sample slices stored at room temperature for 1 day, and then store the two groups of sample slices at room temperature until the continuous action lasts for 7 days and then detect the bactericidal rate. Take the dilution solution, neutralizer, and culture medium of the same batch in the experiment as negative controls. The experiment is repeated 3 times, and the bactericidal rate is calculated.

[0064] 2.3 Calculation of bactericidal rate The bactericidal rate (%) = (the recovered bacteria amount of the control group sample - the recovered bacteria amount of the experimental group sample) / the recovered bacteria amount of the control group sample. Among them, the unit of the bacteria amount is CFU / slice.

[0065] 2.4 Result determination If the bactericidal rate ≥ 90%, it is judged that there is a continuous antibacterial effect during this period.

[0066] Table 3 Persistence of inactivating virus of anti-HIV disinfectant wet wipes (stored for 1 day and 7 days, action time 5 min)

[0067] Table 4 Persistence of antibacterial of anti-HIV disinfectant wet wipes (stored for 1 day and 7 days, action time 5 min) Specification Page 10 / 13 12 CN 121971489 A

[0068] According to Table 3 and Table 4, it can be seen that the anti-HIV disinfectant wet wipes prepared in Examples 1-10, after being wiped on plastic sheets and stored for 1 day and 7 days, the virus inactivation knowledge of the antiviral index (HIV and influenza A virus) for carrier disinfection is greater than 2, and the bactericidal rates (Escherichia coli, Staphylococcus aureus, and Candida albicans) are all ≥ 90%, indicating persistent antibacterial properties. However, for the anti-HIV wet wipes prepared in Comparative Examples 9 and 10, after being stored for 1 day and 7 days, the virus inactivation rates are both less than 1, and the bactericidal rates cannot reach more than 90%, and the persistent antibacterial index cannot be achieved. In addition, the test results show that the dilution solution, neutralizer, and culture medium used in the experiment do not have antiviral and antibacterial properties.

[0069] Test Example 6 1. Materials and methods 1.1. Treatment of test samples The samples are made into spheres with a diameter of about 5 mm.

[0070] 1.2. Experimental animals and feeding environment 1.2.1. Information of experimental animals Species: Hamster; Strain: Golden hamster; Level: SPF level; Number of animals: 3 for each sample; Animal gender: Both male and female are acceptable, and female animals are selected as those that have not been pregnant and have not given birth; Body weight: 106.4 g - 115.7 g; Source: Beijing Vital River Laboratory Animal Technology Co., Ltd.; Experimental animal production license number: SCXK (Beijing) 2021-0011; Quality certificate number: ♂: No. 110011241104944716, ♀: No. 110011241104944858.

[0071] 1.2.2. Information of feeding environment; Facilities: Barrier environment; Specification Page 11 / 13 13 CN 121971489 A Temperature: 24.2 °C - 24.7 °C; Relative humidity: 47.2% - 54.0%; Laboratory animal environment use license number: SYXK (Zhejiang) 2021-0032.

[0072] 1.2.3. Feed information Feed name: Irradiated sterilized laboratory mouse maintenance feed; Feed source: Jiangsu Xietong Pharmaceutical Biotechnology Co., Ltd.; Feed production license number: Su Feed Certificate (2019) 01008; Production date: 2024.04.01; Certificate of conformity number: 20-SM240611026; Batch number: 24040101.

[0073] 1.2.4. Animal drinking water information Water quality: First-stage RO ultrafiltration water, treated with chlorination to control the free chlorine content in the water at 0.3mg / L-2mg / L.

[0074] Water supply method: Directly supplied to animals for free drinking through drinking spouts.

[0075] Analysis and Detection: The heavy metal content, microbial content, and free chlorine content of the drinking water were tested and found to meet the requirements of the "Standards for Drinking Water Quality" (GB 5749-2022).

[0076] 2. Experimental Method: A suitable collar with a width of 3.6 mm was fitted to the animal to allow it to maintain normal eating and breathing while preventing the sample from being removed from the animal's mouth. The animals were weighed daily for 2 consecutive days during the experiment. The prepared test sample was placed in the left cheek pouch with forceps and exposed for 1 hour. The right control group was treated in the same way with cotton balls soaked in sodium chloride injection. After exposure, the collar and cotton balls were removed and the cheek pouch was observed with the naked eye. The cheek pouch was also checked before each exposure. The above steps were repeated every hour for a total of 4 hours. The cheek pouch was observed with the naked eye 24 hours after the last exposure, and the animal was euthanized. The mucosa and surrounding tissue at the contact site of the sample were taken, fixed in 10% formalin, routinely embedded in paraffin, semi-serial sections were prepared, 5 sections were taken at intervals, stained with H&E, and examined under a microscope.

[0077] 3. Evaluation Methods 3.1. Macroscopic Observation Evaluation: Macroscopically observe the mucosa at the sample placement site, noting any congestion, swelling, erosion, or ulceration on the buccal mucosa. Compare the mucosa of the test side and the control side, and score the oral mucosa by macroscopic observation; sum the scores of each animal and divide by the total number of animals observed to obtain the average score for each animal.

[0078] 3.2.Histological evaluation was conducted based on histopathological examination results, scoring the tissue irritation response of the oral mucosa. The irritation response scores of the three animals in the experimental group were summed and then divided by the total number of animals observed to obtain the average score of the oral mucosa irritation response in the experimental group.

[0079] The irritation index was obtained by subtracting the average score of the control group from the average score of the experimental group, and the degree of response was graded. The oral mucosa tissue response was observed visually 24 hours after the last contact with the test sample, and the irritation index grading criteria are shown in Table 5. The results of the oral mucosa tissue irritation index are shown in Table 3.

[0080] Table 5 Oral Mucosal Tissue Reaction Grading Instructions 12 / 13 pages 14 CN 121971489 A

[0081] Table 6 Oral Mucosal Tissue Reaction Irritation Index of Anti-HIV Disinfectant Wipes

[0082] According to Table 6, compared with Example 1, Comparative Examples 2 and 5 lacked matrine extract. The results showed that not only did the virus inactivation and bactericidal properties of the disinfectant decrease significantly, but the disinfectant also had a mild oral mucosal irritation reaction. This indicates that the matrine extract in the disinfectant has a good protective effect on the mucosa and reduces skin irritation. In addition, judging from the oral mucosal irritation reaction index of other comparative examples, matrine extract can reduce the oral mucosal irritation reaction of the disinfectant components nano-silver and compound quaternary ammonium salt.

[0083] Obviously, the above embodiments are merely examples for clear illustration and are not intended to limit the implementation. For those skilled in the art, other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all embodiments here. However, any obvious variations or modifications derived therefrom are still within the scope of protection of this invention. Specification 13 / 13 pages 15 CN 121971489 A Abstract The invention relates to the field of disinfection technology, specifically to a disinfectant solution, disinfectant wipes, and their applications.The disinfectant solution provided by the invention includes the following components: nano-silver, compound quaternary ammonium salts, and sophora alkaloid extract, wherein the compound quaternary ammonium salts are a mixture of single-chain quaternary ammonium salts and double-chain quaternary ammonium salts. The disinfectant solution provided by the invention is composed of nano-silver, sophora alkaloid extract, and compound quaternary ammonium salts formed by a mixture of single-chain and double-chain quaternary ammonium salts. The invention also provides disinfectant wipes made using this disinfectant solution, which can efficiently kill HIV, influenza A virus, as well as bacteria and fungi, with long-lasting antifungal, antibacterial, and antiviral effects, non-irritating to mucous membranes, safe and durable.

Claims

1. A disinfectant, characterized in that, It includes the following components: nano silver, complex quaternary ammonium salt and matrine extract; The composite quaternary ammonium salt is a mixture of single-chain and double-chain quaternary ammonium salts.

2. The disinfectant according to claim 1, characterized in that, The mass ratio of single-chain quaternary ammonium salt to double-chain quaternary ammonium salt in the composite quaternary ammonium salt is 1.0:0.5-2, preferably 1.0:0.8-1.4; The matrine extract was extracted using an ethanol extraction method.

3. The disinfectant according to claim 1, characterized in that, In the disinfectant, the content of nano silver is 5-10 mg / L, the content of single-chain quaternary ammonium salt is 0.005-0.02 wt%, the content of double-chain quaternary ammonium salt is 0.005-0.02 wt%, and the content of matrine extract is 3-5 vol.

4. The disinfectant according to claim 1, characterized in that, The single-chain quaternary ammonium salt includes at least one of dodecyl dimethyl benzyl ammonium chloride, dodecyl dimethyl benzyl ammonium bromide, hexadecyl trimethyl ammonium chloride, and hexadecyl trimethyl ammonium bromide; The double-chain quaternary ammonium salt includes at least one of dialcyldimethylammonium chloride, dialcyldimethylammonium bromide, dioctyldimethylammonium chloride, and dioctyldimethylammonium bromide.

5. The disinfectant according to claim 1, characterized in that, The disinfectant also includes ethanol, surfactants, and solubilizers.

6. The disinfectant according to claim 5, characterized in that, The surfactant includes at least one of polysorbate 80 or polyoxyethylene castor oil; The solubilizer includes at least one of ethylene glycol or propylene glycol.

7. The disinfectant according to claim 5, characterized in that, Based on the total weight of the disinfectant, the ethanol content is 11.25-15 v / v, the surfactant content is 1-2 v / v, and the solubilizer content is 0.5-1 v / v.

8. A disinfectant wipe, characterized in that, Includes the disinfectant as described in any one of claims 1-7.

9. The disinfectant wipes according to claim 8, characterized in that, The ratio of the disinfectant to the nonwoven fabric substrate is 3.0-3.5:1, in mL:g; The nonwoven fabric substrate includes viscose and polyester, and the viscose content is 40-45 wt% and the polyester content is 55-60 wt% based on the total weight of the nonwoven fabric substrate.

10. The use of a disinfectant according to any one of claims 1-7 or a disinfectant wipe according to any one of claims 8-9 in the treatment of HIV, influenza A virus, bacteria, and fungi.