Sample imaging method, apparatus, storage medium, device, and program product

Abstract

The invention discloses a sample imaging method and device, a storage medium, equipment and a program product. The method is applied to the electrophoresis chip, the electrophoresis chip is provided with a plurality of flow channels used for containing samples to be detected, and the method comprises the following steps: sequentially carrying out sample adding operation on each flow channel in the flow channels, carrying out electrophoresis operation on the flow channels subjected to the sample adding operation so as to enable the samples to be detected in the flow channels to be subjected to electrophoretic separation, and after the (N-1) th flow channel completes the sample adding operation, carrying out electrophoresis separation on the (N-1) th flow channel, carrying out sample adding operation on the Nth flow channel, carrying out electrophoresis operation on the (N-1) th flow channel, carrying out electrophoresis operation on the Nth flow channel after the sample adding operation on the Nth flow channel is completed, N is more than 1 and less than or equal to the total number of the flow channels, and N is a natural number; and photographing each flow channel after the electrophoresis operation is completed so as to obtain a sample imaging image corresponding to each flow channel. Through parallel sample adding operation and electrophoresis operation, the total time required by sample imaging is effectively shortened, the efficiency and continuity of a sample imaging process are improved, and the detection quality is improved.

Description

(19) State Intellectual Property Office (12) Invention Patent Application (10) Application Publication Number (43) Application Publication Date (21) Application Number 202511235003.8 (22) Application Date 2025.08.29 (71) Applicant Shenzhen Zhenmai Biotechnology Co., Ltd. Address 518023 No. 112, Qingshuihe Road, Qingshuihe Community, Qingshuihe Street, Luohu District, Shenzhen, Guangdong Province, 502A and 502B, Podium Building 602 (72) Inventors Xia Xiangtao, Wu Ping, Zhou Zhiliang (74) Patent Agency Beijing Qingyihua Intellectual Property Agency (General Partnership) 11201 Patent Attorney Xu Zhangwei (51) Int.Cl. G01N 27 / 447 (2006.01) G01N 21 / 84 (2006.01) G01N 35 / 10 (2006.01) (54) Invention Title: Sample Imaging Method, Apparatus, Storage Medium, Device, and Program Product (57) Abstract: This application discloses a sample imaging method, apparatus, storage medium, device, and program product. The method is applied to an electrophoresis chip, which has multiple channels for accommodating samples to be tested. The method includes: sequentially adding samples to each channel and performing electrophoresis on the channels after the sample addition operation, so that the samples to be tested in the channels are separated by electrophoresis. Specifically, after the sample addition operation is completed in the (N-1)th channel, the sample addition operation is performed in the Nth channel, and the electrophoresis operation is performed in the (N-1)th channel. After the sample addition operation is completed in the Nth channel, the electrophoresis operation is performed in the Nth channel, where 1 < N ≤ the total number of channels, and N is a natural number. Each channel that has completed the electrophoresis operation is photographed to obtain a sample imaging image corresponding to each channel. This application effectively shortens the total time required for sample imaging by parallel sample addition and electrophoresis operations, improves the efficiency and continuity of the sample imaging process, and improves the detection quality. Claims 2 pages, Description 26 pages, Drawings 12 pages, CN 121347633 A 2026.01.16 CN 1 21 34 76 33 A 1. A sample imaging method, characterized in that it is applied to an electrophoresis chip, the electrophoresis chip having a plurality of channels for accommodating samples to be tested, the method comprising: sequentially performing a sample loading operation on each channel in the channels, and performing an electrophoresis operation on the channels after the sample loading operation is completed, so that the samples to be tested in the channels are separated by electrophoresis, wherein, after the sample loading operation is completed in the (N-1)th channel, a sample loading operation is performed on the Nth channel, and an electrophoresis operation is performed on the (N-1)th channel; after the sample loading operation is completed in the Nth channel, an electrophoresis operation is performed on the Nth channel, 1 < N ≤ the total number of channels, N is a natural number; taking a picture of each channel that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel.2. The sample imaging method as claimed in claim 1, characterized in that each channel in the electrophoresis chip is misaligned with the corresponding electrode contact end; when the probe of the electrophoresis mechanism for performing electrophoresis contacts the corresponding electrode contact end of each channel in the electrophoresis chip, the moving path of the sample dispensing needle of the sample dispensing mechanism for performing sample dispensing does not overlap with the moving path of the probe in spatial projection. 3. The sample imaging method as claimed in claim 2, characterized in that, for non-edge channels, the corresponding electrode contact end is located in the interval region between adjacent channels; for edge channels, the corresponding electrode contact end is located on the side of the corresponding channel away from adjacent channels, or the electrode contact end corresponding to one edge channel is located on the side of the corresponding channel away from adjacent channels, and the electrode contact end corresponding to another edge channel is located in the interval region between the other edge channel and adjacent channels, so that each channel in the electrophoresis chip is misaligned with the corresponding electrode contact end. 4. The sample imaging method as described in claim 2, characterized in that the electrophoresis mechanism includes M probe groups corresponding to the total number of channels, each probe group including a positive probe and a negative probe, and the electrophoresis operation on the channels after sample loading includes: before the sample loading operation of the first channel is completed, controlling all probe groups of the electrophoresis mechanism to simultaneously contact the electrode contact end of the corresponding channel; after the sample loading operation of the (N-1)th channel is completed, controlling the probe group corresponding to the (N-1)th channel to be energized to perform electrophoresis on the (N-1)th channel; after the sample loading operation of the Nth channel is completed, controlling the probe group corresponding to the Nth channel to be energized to perform electrophoresis on the Nth channel. 5. The sample imaging method as described in claim 1, characterized in that, taking a picture of each channel after the electrophoresis operation has been completed to obtain a sample imaging image corresponding to each channel includes: taking a picture of the (N-1)th channel after the electrophoresis operation has been completed in the (N-1)th channel to obtain a sample imaging image corresponding to the (N-1)th channel; and taking a picture of the Nth channel after the electrophoresis operation has been completed in the Nth channel to obtain a sample imaging image corresponding to the Nth channel. 6. The sample imaging method as described in claim 4, characterized in that the imaging mechanism has an imaging field of view covering K adjacent channels, K≥2; the method further includes: according to the electrophoresis progress prediction, controlling the imaging mechanism to move in advance to a first imaging region containing channels i to j, wherein the first imaging region simultaneously covers K adjacent channels, 1≤i<M-K, j=i+K-1; when it is detected that any channel in the first imaging region has completed the electrophoresis operation, controlling the imaging mechanism to take a picture of the channel in the first imaging region; if other channels in the first imaging region have not completed the electrophoresis operation, then maintaining the position of the imaging mechanism to continue... (See claim 1 / 2 page 2 CN 121347633 A)After all channels in the first imaging region have been photographed, the imaging mechanism is moved to the second imaging region according to the position of the channels that will be electrophoresed in the next batch. 7. A sample imaging device, characterized in that it is applied to an electrophoresis chip, the electrophoresis chip having a plurality of channels for accommodating samples to be tested, the device comprising: a processing unit, configured to sequentially perform a sample loading operation on each channel in the channels, and perform an electrophoresis operation on the channels that have completed the sample loading operation, so that the samples to be tested in the channels are separated by electrophoresis, wherein, after the sample loading operation is completed in the (N-1)th channel, the sample loading operation is continued in the Nth channel, and the electrophoresis operation is performed in the (N-1)th channel; after the sample loading operation is completed in the Nth channel, the electrophoresis operation is performed in the Nth channel, 1 < N ≤ the total number of channels, N is a natural number; and an acquisition unit, configured to photograph each channel that has completed the electrophoresis operation to acquire a sample imaging image corresponding to each channel. 8. A computer-readable storage medium, characterized in that the computer-readable storage medium stores a computer program adapted for loading by a processor to execute the sample imaging method according to any one of claims 1-6. 9. A computer device, characterized in that the computer device includes a processor and a memory, the memory storing a computer program, the processor executing the sample imaging method according to any one of claims 1-6 by calling the computer program stored in the memory. 10. A computer program product, including computer instructions, characterized in that the computer instructions, when executed by a processor, implement the sample imaging method according to any one of claims 1-6. Claims 2 / 2 Page 3 CN 121347633 A Sample Imaging Method, Apparatus, Storage Medium, Device and Program Product Technical Field

[0001] This application relates to the field of biological detection technology, specifically to a sample imaging method, apparatus, storage medium, device and program product. Background Art

[0002] With the increasing application of nucleic acid fragment quality control in more and more industries and fields, its quality control technology is also constantly developing. Commonly used nucleic acid fragment separation and detection techniques are based on electrophoresis chips, which are widely used due to their simplicity and intuitive results. However, traditional electrophoresis methods suffer from problems such as long processing time and unstable detection quality. Especially in fully automated electrophoresis processes, sample aspiration, sample loading, electrophoresis, and imaging are usually performed sequentially, resulting in low overall detection efficiency. Furthermore, prolonged standing of samples in buffer solution may cause diffusion of nucleic acid fragments, affecting the clarity and resolution of electrophoretic bands.

[0003] This application provides a sample imaging method, apparatus, storage medium, device, and program product that effectively shortens the total time required for sample imaging and improves the efficiency of the sample imaging process by performing sample loading and electrophoresis operations in parallel.To improve the continuity and detection quality.

[0004] On one hand, this application provides a sample imaging method applied to an electrophoresis chip, the electrophoresis chip having multiple channels for accommodating samples to be tested, the method comprising:

[0005] sequentially performing a sample loading operation on each channel in the channels, and performing an electrophoresis operation on the channels after the sample loading operation is completed, so that the samples to be tested in the channels are separated by electrophoresis, wherein, after the sample loading operation is completed in the (N-1)th channel, a sample loading operation is performed on the Nth channel, and an electrophoresis operation is performed on the (N-1)th channel, and after the sample loading operation is completed in the Nth channel, an electrophoresis operation is performed on the Nth channel, 1 < N ≤ the total number of channels, N is a natural number;

[0006] taking a picture of each channel that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel.

[0007] On the other hand, embodiments of this application provide a sample imaging device applied to an electrophoresis chip, the electrophoresis chip having multiple channels for accommodating samples to be tested, the device comprising:

[0008] a processing unit, configured to sequentially perform sample loading operations on each channel in the channels, and perform electrophoresis operations on the channels after the sample loading operations have been completed, so that the samples to be tested in the channels are separated by electrophoresis, wherein, after the sample loading operation is completed on the (N-1)th channel, the sample loading operation is continued on the Nth channel, and the electrophoresis operation is performed on the (N-1)th channel, and after the sample loading operation is completed on the Nth channel, the electrophoresis operation is performed on the Nth channel, 1 < N ≤ the total number of channels, N is a natural number;

[0009] an acquisition unit, configured to take pictures of each channel that has completed the electrophoresis operation, so as to acquire a sample imaging image corresponding to each channel.

[0010] On the other hand, embodiments of this application provide a computer-readable storage medium storing a computer program adapted for loading by a processor to execute the sample imaging method described in any of the above embodiments.

[0011] On the other hand, embodiments of this application provide a computer device including a processor and a memory storing a computer program. The processor executes the sample imaging method described in any of the above embodiments by calling the computer program stored in the memory.

[0012] On the other hand, embodiments of this application provide a computer program product including computer instructions that, when executed by a processor, implement the sample imaging method described in any of the above embodiments.

[0013] The sample imaging method provided in this application is applied to an electrophoresis chip. The electrophoresis chip has multiple channels for accommodating samples to be tested. In this method, a sample is added sequentially to each channel, and the sample is processed after addition.Electrophoresis is performed on the flow channels to separate the test samples within the channels. After the sample loading operation is completed in the (N-1)th flow channel, the sample loading operation is performed on the Nth flow channel, and electrophoresis is performed on the (N-1)th flow channel. After the sample loading operation is completed in the Nth flow channel, electrophoresis is performed on the Nth flow channel. 1 < N ≤ the total number of flow channels, where N is a natural number. Each flow channel that has completed electrophoresis is photographed to obtain a sample imaging image corresponding to each flow channel. This embodiment of the application effectively shortens the total time required for sample imaging by performing the sample loading and electrophoresis operations in parallel, improving the efficiency and continuity of the sample imaging process and enhancing the detection quality. Brief Description of the Drawings

[0014] To more clearly illustrate the technical solutions in the embodiments of this application, the drawings used in the following description of the embodiments will be briefly introduced. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] In order to more clearly illustrate the technical solutions in the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below.

[0016] Figure 1 is a schematic flowchart of the sample imaging method provided in the embodiment of this application;

[0017] Figure 2 is a schematic diagram of the analysis device provided in the embodiment of this application;

[0018] Figure 3 is a partial enlarged view of part A of Figure 2 provided in the embodiment of this application;

[0019] Figure 4 is a schematic diagram of the fixing plate provided in the embodiment of this application;

[0020] Figure 5 is a schematic diagram of the electrophoresis fixing plate provided in the embodiment of this application;

[0021] Figure 6 is a schematic diagram of the imaging mechanism provided in the embodiment of this application;

[0022] Figure 7 is a schematic diagram of the analysis device provided in the embodiment of this application from another perspective;

[0023] Figure 8 is a partial enlarged view of part B of Figure 7 provided in the embodiment of this application;

[0024] Figure 9 is a schematic diagram of the baffle provided in the embodiment of this application;

[0025] Figure 10 is a schematic diagram of the movement of the pusher and the baffle provided in the embodiment of this application;

[0026] Figure 11 is a schematic diagram of the sample loading mechanism provided in the embodiment of this application;

[0027] Figure 12 is a partially enlarged view of part C of Figure 11 provided in an embodiment of this application;

[0028] Figure 13 is a schematic diagram of an electrophoretic chip substrate provided in an embodiment of this application;

[0029] Figure 14 is a schematic diagram of a first type of printed electrode layer projected orthogonally onto a first surface of an electrophoretic chip substrate provided in an embodiment of this application;

[0030] Figure 15 is a schematic diagram of a second type of printed electrode layer projected orthogonally onto a first surface of an electrophoretic chip substrate provided in an embodiment of this application;

[0031] Figure 16 is a schematic diagram of the second surface of an electrophoretic chip substrate provided in an embodiment of this application;

[0032] Figure 17 is a schematic diagram of the structure of the electrophoresis chip cover plate provided in the embodiment of this application, on the fourth surface of which a first type of printed electrode layer is provided (page 2 / 26, CN 121347633 A);

[0033] Figure 18 is a schematic diagram of the structure of the electrophoresis chip cover plate provided in the embodiment of this application, on the fourth surface of which a second type of printed electrode layer is provided;

[0034] Figure 19 is a schematic diagram of the third surface of the electrophoresis chip cover plate provided in the embodiment of this application;

[0035] Figure 20 is a schematic diagram of another electrophoresis chip provided in the embodiment of this application;

[0036] Figure 21 is a schematic diagram of the substrate of another electrophoresis chip provided in the embodiment of this application;

[0037] Figure 22 is a partial enlarged view of part D of Figure 21 provided in the embodiment of this invention;

[0038] Figure 23 is a timing diagram of the concurrent full-process electrophoresis step breakdown provided in the embodiment of this application;

[0039] Figure 24 is a schematic diagram of the structure of the sample imaging device provided in the embodiment of this application;

[0040] Figure 25 is a schematic diagram of the structure of the computer device provided in the embodiment of this application. Detailed Description of Embodiments

[0041] The technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0042] The following will provide detailed descriptions. It should be noted that the order of description of the following embodiments is not intended to limit the priority of the embodiments.

[0043] As nucleic acid fragment quality control is applied in more and more industries and fields, its quality control technology is also constantly developing. The commonly used nucleic acid fragment separation and detection technology is electrophoresis based on electrophoresis chips, which is widely used because of its simple operation and intuitive results. However, traditional electrophoresis methods have problems such as long processing time and unstable detection quality. Especially in fully automated electrophoresis, the steps of sample aspiration, sample loading, electrophoresis, and photography are usually performed sequentially, resulting in low overall detection efficiency. Moreover, the long-term static placement of samples in buffer may cause diffusion of nucleic acid fragments, affecting the clarity and resolution of electrophoretic bands. Specifically, the traditional fully automated process is broken down as follows:

[0044] (1) Place the sample to be tested and the chip;

[0045] (2) Select the number of electrophoresis channels;

[0046] (3) Detect the number of available channels on the chip;

[0047] (4) Detect the number of available pipette tips;

[0048] (5) Press the chip to the puncture site and perform puncture sequentially;

[0049] (6) Use a pipette to retrieve the pipette tips;

[0050] (7) Use a pipette to aspirate the sample;

[0051] (8) Use a pipette to add the sample;

[0052] (9) For each flow channel, the pipette tip needs to be taken, the sample aspirated, and the sample added. This process is repeated for all flow channels in sequence.

[0053] (10) Press the chip into the electrophoresis position, and perform electrophoresis on all flow channels simultaneously.

[0054] (11) After electrophoresis, move the chip sequentially from the first detection flow channel to take pictures.

[0055] (12) The algorithm processes and analyzes the images after recognition.

[0056] (13) Output the results.

[0057] According to the above execution steps, each flow channel needs to repeat the three steps of taking the pipette tip, aspirating the sample, and loading the sample. After adding the sample to the first flow channel and taking pictures of the last flow channel, a long waiting time is required. If the sample is left to stand for too long, it will cause the nucleic acid fragment molecules in the sample to move and diffuse, spreading throughout the buffer chamber and the gel. The sample bands will not be sufficiently aggregated. As a result, during electrophoresis, phenomena such as band dispersion, band "blurring," and "tailing" occur, and the bands are no longer in the optimal aggregation state. At this time, the sample results obtained after processing the band images obtained by the algorithm will also be distorted.

[0058] In addition, in traditional fully automated electrophoresis, the four actions of sample aspiration, sample loading, electrophoresis, and photography are performed sequentially. Each action requires the completion of all electrophoresis channels before proceeding to the next step. The subsequent channels need to wait for the preceding channels to complete the entire process, and the overall time required will also increase as the number of electrophoresis channels increases.

[0059] Therefore, this application embodiment provides a sample imaging method that can effectively shorten the total time required for sample imaging by performing sample loading and electrophoresis operations in parallel, improve the efficiency and continuity of the sample imaging process, and improve the detection quality.

[0060] As shown in Figure 1, this application embodiment provides a sample imaging method, which can be applied to an analytical device and an electrophoresis chip. Before describing the sample imaging method, the analytical device and the electrophoresis chip will be introduced first. Please refer to Figures 2 to 12. The analytical apparatus 10 includes:

[0061] a base plate 100, the base plate 100 having a first cavity 110;

[0062] a chip fixing mechanism 200, disposed on the base plate 100, including at least one analytical station located in the first cavity 110, the analytical station being used to vertically place an electrophoresis chip 2000, the electrophoresis chip 2000 having a plurality of channels 2140 for accommodating samples to be tested;

[0063] a sample loading mechanism 600, used to sequentially load samples onto each of the plurality of channels 2140 disposed in the electrophoresis chip 2000;

[0064] an electrophoresis mechanism 300, disposed on the base plate 100, used to perform electrophoresis on the channels 2140 after the sample loading operation, so that the samples to be tested within the channels 2140 are separated by electrophoresis; and

[0065] An imaging mechanism 400, mounted on a base plate 100, is used to take pictures of each channel 2140 that has completed the electrophoresis operation, so as to obtain an image of the sample corresponding to each channel 2140.

[0066] In some embodiments, the analysis device 10 further includes a blocking mechanism 500 disposed in the first cavity 110 and corresponding to the location of the analysis station, including at least one recycling station.

[0067] In this embodiment, when the electrophoresis chip 2000 is located on the analysis station, the chip fixing mechanism 200 fixes the electrophoresis chip 2000, and the blocking mechanism 500 prevents the electrophoresis chip 2000 from falling. The sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 in the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 in the electrophoresis chip 2000 that have completed the sample loading operation, so that the samples to be tested in the channels 2140 are separated by electrophoresis. The imaging mechanism 400 takes pictures of each channel that has completed the electrophoresis operation to obtain the sample imaging image corresponding to each channel. Finally, the chip fixing mechanism 200 releases the electrophoresis chip 2000, and the blocking mechanism 500 moves, so that the electrophoresis chip 2000 falls to the recycling station.

[0068] The base plate 100 can be designed as a square structure. The chip fixing mechanism 200, the electrophoresis mechanism 300, the imaging mechanism 400 and the blocking mechanism 500 are arranged on the base plate 100. Multiple support feet are provided on the lower surface of the base plate 100 to provide support for the base plate 100.

[0069] In this embodiment, the electrophoresis chip 2000 can be directly placed at the analysis station of the chip fixing mechanism 200, or it can be placed at the entrance of the analysis device 10. A moving mechanism, such as a robotic arm or conveyor belt, can move the electrophoresis chip 2000 from the entrance to the analysis station of the chip fixing mechanism 200. When the electrophoresis chip 2000 is at the analysis station, the chip fixing mechanism 200 fixes the electrophoresis chip 2000 to prevent it from moving during subsequent electrophoretic separation and imaging. At this time, the blocking mechanism 500 is in its initial position, preventing the electrophoresis chip 2000 from falling. (See page 4 / 26 of the instruction manual, CN 121347633 A 2000). After the chip fixing mechanism 200 fixes the electrophoresis chip 2000, the sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 within the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 within the electrophoresis chip 2000 where samples have been loaded, allowing the test samples within the channels 2140 to be separated by electrophoresis. The imaging mechanism 400 takes a picture of each channel 2140 that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel 2140. The obtained sample imaging images are then analyzed to determine information such as the fragment length, concentration, and integrity of the test sample. After the analysis is completed, the chip fixing mechanism 200 releases the electrophoresis chip 2000 and stops electrophoresis.The electrophoresis chip 2000 is fixed, allowing it to move. The blocking mechanism 500 moves from its initial position, and the electrophoresis chip 2000 falls to the recycling station after losing the blocking effect of the blocking mechanism 500. After the analysis and recycling of one electrophoresis chip 2000 is completed, the same operation is performed on the next electrophoresis chip 2000 until the analysis and recycling of all electrophoresis chips 2000 are completed.

[0070] The analysis device 10 provided in this application can perform electrophoretic analysis on samples containing biological substances such as DNA, RNA, or proteins using electrophoresis technology. In this document, "sample" and "sample to be analyzed" both refer to the same thing, namely, the sample loaded into the electrophoresis chip 2000 for analysis.

[0071] For example, taking a nucleic acid sample containing DNA or RNA as the sample to be tested, when the electrophoresis chip 2000 is in the analysis station, after the chip fixing mechanism 200 fixes the electrophoresis chip 2000, the sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 in the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 in the electrophoresis chip 2000 where the sample loading operation has been completed, so that the sample to be tested in the channels 2140 can be separated by electrophoresis. That is, the electrophoresis mechanism 300 applies voltage to the nucleic acid sample in the channels 2140 in the electrophoresis chip 2000 where the sample loading operation has been completed. The nucleic acid sample moves under the action of the electric field force. Since there are nucleic acid fragments of different lengths in the nucleic acid sample, differential separation of nucleic acid fragments of different lengths is achieved, forming multiple bands. After the nucleic acid samples undergo electrophoretic separation, the imaging mechanism 400 photographs each channel 2140 that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel 2140. The photographed sample imaging images are then analyzed to determine information such as the length, concentration, and integrity of the nucleic acid fragments corresponding to each band in the nucleic acid sample. After the analysis is complete, the chip fixing mechanism 200 releases the electrophoresis chip 2000, the blocking mechanism 500 moves, and the electrophoresis chip 2000 falls to the recycling station. For samples containing other biological materials, the same process can be performed: sample addition by the sample addition mechanism 600, electrophoresis separation by the electrophoresis mechanism 300, and imaging by the imaging mechanism 400. Finally, the photographed sample imaging images are analyzed to complete the analysis process.

[0072] Further, a molecular weight standard (Ladder) is provided. The nucleic acid sample and the molecular weight standard are separated by electrophoresis under the action of an electric field. In subsequent analysis based on the obtained images, the standard reference band separated by the molecular weight standard is used as a benchmark. The information of the standard reference band separated by the molecular weight standard and the information separated by the nucleic acid sample (i.e., the brightness and position information of the band objects in the sample imaging image) are compared to calculate the nucleic acid sample corresponding to each channel 2140.The band information in the (sample to be tested) includes information such as the length, concentration, and nucleic acid integrity index of the corresponding nucleic acid fragment. Of course, it is possible to calculate the length, concentration, and nucleic acid integrity index of the nucleic acid fragment corresponding to the band object separated from the nucleic acid sample directly based on the information of the nucleic acid sample after electrophoresis without setting molecular weight standards.

[0073] In this embodiment, as shown in Figures 2, 7, 8, 9, and 10, the blocking mechanism 500 includes: a baffle 520 disposed in the first cavity 110 and corresponding to the position of the analysis station; and a first driving member 510 driven by the baffle 520.

[0074] The first driving member 510 can drive the baffle 520 to move. If the first driving member 510 is a motor, the baffle 520 is provided with a protrusion 522, and the output shaft of the motor is connected to the protrusion 522. When the motor is working, it drives the protrusion 522 to move through the output shaft, thereby driving the baffle 520 to move. To save space, the first driving member 510 is disposed below the base plate 100. Instruction manual, page 5 / 26, CN 121347633 A

[0075] The baffle 520 is designed with a square structure. Regardless of whether the electrophoresis chip 2000 is placed vertically or horizontally at the analysis station, the baffle 520 can prevent the electrophoresis chip 2000 from falling. After the baffle 520 moves, it loses its blocking effect and the electrophoresis chip 2000 can fall to the recycling station. The baffle 520 is set in the first cavity 110, and the horizontal surface of the baffle 520 is flush with the first cavity 110, so that the moving contact plane of the electrophoresis chip 2000 will not be uneven during the movement, and the movement can be carried out smoothly.

[0076] When the electrophoresis chip 2000 is placed at the analysis station, the baffle 520 is in the initial position and can block the electrophoresis chip 2000, preventing the electrophoresis chip 2000 from falling. After the electrophoresis chip 2000 completes analysis, the first driving component 510 drives the baffle 520 to move, and the electrophoresis chip 2000 loses the blocking effect of the baffle 520 and falls to the recycling station.

[0077] In some specific embodiments of this application, referring to Figures 2 and 7, the blocking mechanism 500 also includes a bending plate 530 with a certain bending angle disposed in the first cavity 110 and corresponding to the position of the analysis station. When the electrophoresis chip 2000 falls, it falls to the recycling station along the bending plate 530.

[0078] The bending plate 530 has through holes 211 on both sides. By passing screws through the through holes 211, the bending plate 530 can be fixedly connected to the inner wall of the first cavity 110. The bending plate 530 is made of a material that is not easily deformed, such as iron, steel, or aluminum alloy. The bending plate 530 includes a first surface and a second surface, and a certain bending angle is formed between the first surface and the second surface, such as 50°, 60°, 70° or other angles.After the sample to be analyzed in the electrophoresis chip 2000 is completed, the first driving component 510 drives the baffle 520 to move, and the electrophoresis chip 2000 loses the blocking effect of the baffle 520 and begins to fall. The electrophoresis chip 2000 first falls to the first surface, and then falls along the first surface and the second surface to the recycling station, or first falls to the second surface, and then falls along the second surface to the recycling station.

[0079] The bending plate 530 can provide a certain guiding effect to prevent the electrophoresis chip 2000 from falling to the recycling station smoothly. And the bending plate 530 can provide a certain buffering effect to prevent the electrophoresis chip 2000 from being damaged when it falls from top to bottom to the recycling station, and prevent the sample to be analyzed in the electrophoresis chip 2000 from leaking out.

[0080] In some specific embodiments of this application, the recycling station is provided with a waste chip bin. After the sample to be analyzed in the electrophoresis chip 2000 is completed, the blocking mechanism 500 moves, and the electrophoresis chip 2000 falls into the waste chip bin of the recycling station. After all the electrophoresis chips 2000 have completed analysis or after a period of time, the user can recycle the electrophoresis chips 2000 that have completed analysis in the waste chip bin.

[0081] In this embodiment, as shown in Figures 2 to 4, the chip fixing mechanism 200 includes: a fixing plate 210 and a pushing mechanism that provides a pushing force toward the electrophoresis chip 2000 placed on the fixing plate. The fixing plate 210 includes through holes 211 provided on the first side and the second side of the fixing plate 210. Under the pushing of the pushing mechanism, the electrophoresis chip 2000 is placed close to the fixing plate 210.

[0082] Wherein, the fixing plate 210 is fixedly set on the base plate 100, the electrophoresis chip 2000 is set vertically, and under the pushing of the pushing mechanism, the electrophoresis chip 2000 is placed close to the fixing plate 210. One electrophoresis chip 2000 may be provided. One side of the electrophoresis chip 2000 is pushed by the pushing mechanism, and the other side is subjected to the force of the fixing plate 210. Multiple electrophoresis chips 2000 may be provided, and each electrophoresis chip 2000 is attached to each other. One electrophoresis chip 2000 in contact with the pushing mechanism is pushed by the pushing mechanism, and the other electrophoresis chip 2000 in contact with the fixing plate 210 is placed tightly against the fixing plate 210 under the push of the pushing mechanism.

[0083] When the electrophoresis chip 2000 is initially in the analysis station, the electrophoresis chip 2000 may be in a slightly tilted state. The second driving member 221 drives the pushing member 222 to move. The pushing member 222 provides a pushing force to the electrophoresis chip 2000. The electrophoresis chip 2000 is pushed on one side and subjected to the force of the fixing plate 210 on the other side, and rotates slightly to become vertical and is placed tightly against the fixing plate 210.

[0084] When one electrophoresis chip 2000 is provided, the electrophoresis chip 2000 is placed vertically, and the second driving unit 221 drives and pushes it.When component 222 moves, the pushing component 222 provides a thrust to one side of the electrophoresis chip 2000, while the other side of the electrophoresis chip 2000 is in close contact with the fixing plate 210. After the sample to be tested inside the electrophoresis chip 2000 has been analyzed, the second driving component 221 drives the pushing component 222 to move, and the electrophoresis chip 2000 loses the thrust provided by the pushing component 222 and is in a released state. The blocking mechanism 500 moves, causing the electrophoresis chip 2000 to fall to the recycling station. Then, the next electrophoresis chip 2000 is placed, and the next electrophoresis chip 2000 is analyzed and recycled.

[0085] When multiple electrophoresis chips 2000 are provided, the multiple electrophoresis chips 2000 are placed vertically. The second driving member 221 drives the pushing member 222 to move. The pushing member 222 provides a pushing force to the electrophoresis chip 2000 in contact with it. The fixing plate 210 provides a force to the electrophoresis chip 2000 in contact with it. The electrophoresis chips 2000 are in close contact with each other, and the position of the electrophoresis chip 2000 closest to the fixing plate 210 is the analysis station. After the sample to be tested in the electrophoresis chip 2000 in the analysis station is analyzed, the second driving member 221 drives the pushing member 222 to move, the electrophoresis chip 2000 in the analysis station is in a released state, the blocking mechanism 500 moves, so that the electrophoresis chip 2000 in the analysis station falls to the recycling station. The blocking mechanism 500 moves and resets, and the second driving member 221 drives the pushing member 222 to move again, so that the remaining electrophoretic chips 2000 are in close contact with each other, and the next electrophoretic chip 2000 moves to the analysis station. The above operation is repeated so that the next electrophoretic chip 2000 also completes the analysis and recycling work. Until all electrophoretic chips 2000 have completed the analysis and recycling work, the next batch of electrophoretic chips 2000 is placed and analyzed and recycled.

[0086] In other embodiments, the chip fixing mechanism 200 can also be other design structures. For example, the chip fixing mechanism 200 is a gripper. When the electrophoretic chip 2000 is in the analysis station, the gripper clamps the electrophoretic chip 2000 to fix the electrophoretic chip 2000; after the electrophoretic chip 2000 completes the analysis, the gripper releases the electrophoretic chip 2000, the blocking mechanism 500 moves, and the electrophoretic chip 2000 in the analysis station falls to the recycling station.

[0087] In this embodiment, as shown in Figures 7 and 10, the pushing mechanism includes a second driving member 221 and a pushing member 222. The pushing member 222 includes a straight plate 2221 and a vertical plate 2222. The straight plate 2221 is drivenly connected to the second driving member 221. One end of the vertical plate 2222 is fixedly connected to the straight plate 2221, and the other end of the vertical plate 2222 passes through the first cavity 110 and is placed in the chip fixing mechanism 200.The electrophoretic chip 2000 on the fixed plate 210 is in contact. The second driving member 221 can drive the pushing member 222 to move. If the second driving member 221 is a motor, the output shaft of the motor is connected to the straight plate 2221. When the motor is working, it drives the straight plate 2221 to move through the output shaft, thereby driving the pushing member 222 to move as a whole. The pushing member 222 provides thrust to the electrophoretic chip 2000 in contact with it.

[0088] To save space, the second driving member 221 is set below the base plate 100.

[0089] Referring to Figures 9 to 10, the baffle 520 is designed as a square structure. The two sides of the baffle 520 can prevent the electrophoretic chip 2000 from falling. The baffle 520 has a through cavity 521 inside. When the upright plate 2222 of the pushing member 222 passes through the first cavity 110, it also passes through the through cavity 521 of the baffle 520, so that the pushing member 222 can move freely without being affected by the baffle 520.

[0090] Referring to FIG10, the discarding process of the electrophoresis chip 2000 is described in detail here. When the electrophoresis chip 2000 is in the analysis station, the second driving member 221 drives the pushing member 222 to move in the opposite direction of the a direction, so that each electrophoresis chip 2000 is in close contact with each other. The electrophoresis mechanism 300 performs electrophoretic separation on the sample to be tested in the electrophoresis chip 2000 in the analysis station, and the imaging mechanism 400 then takes a picture of the sample to be tested. Then the second driving member 221 drives the pushing member 222 to move in the a direction, and the electrophoresis chip 2000 loses the thrust provided by the pushing member 222. The first driving member 510 drives the baffle 520 to move in the a direction, so that the electrophoresis chip 2000 falls to the recycling station.

[0091] In this embodiment, referring to FIG4, the fixing plate 210 includes through holes 211 provided on the first side and the second side. Referring to FIG5, the electrophoresis mechanism 300 includes an electrophoresis fixing plate 310 and a probe. Referring to Figure 3, the electrophoresis fixing plate 310 is adjacent to the fixing plate 210 of the chip fixing mechanism 200: the first and second sides of the electrophoresis fixing plate 310 are provided with probe holes 311 for accommodating probes, and the probe holes 311 correspond to the through holes 211 on the fixing plate 210 of the chip fixing mechanism 200.

[0092] In some embodiments, the first and second sides of the fixing plate 210 are respectively the upper and lower sides of the fixing plate 210, respectively.

[0093] The electrophoresis fixing plate 310 is fixedly disposed on the base plate 100 and is disposed adjacent to the fixing plate 210. The probes are fixedly disposed in the probe holes 311 on the first and second sides of the electrophoresis fixing plate 310 and pass through the through holes 211 on the first and second sides of the fixing plate 210.

[0094] When the electrophoresis chip 2000 is in the analysis station, the probe contacts the electrophoresis chip 2000 and applies an electrophoresis agent to the electrophoresis chip 2000.A voltage is applied, thereby causing the sample to be tested within the electrophoresis chip 2000 to be electrophoretically separated.

[0095] In this embodiment, referring to FIG6, the imaging mechanism 400 in the analysis device 10 includes a camera 410 and at least one light source 420. After the electrophoresis mechanism 300 performs electrophoretic separation on the sample to be tested within the electrophoresis chip 2000, the light source 420 provides light, the camera 410 takes a picture of the sample to be tested within the electrophoresis chip 2000, and analyzes the picture.

[0096] In this embodiment, two light sources 420 are provided, emitting light of different wavelengths. In some embodiments, one light source 420 emits light of a first wavelength, exciting a first optically detectable mark on the sample to be tested to generate a first optical signal, and the camera 410 takes a picture of the sample to be tested to collect the first optical signal for imaging. The other light source 420 emits light of a second wavelength, exciting a second optically detectable mark on the ladder to generate a second optical signal, and the camera 410 takes a picture of the ladder to collect the second optical signal for imaging. The first optically detectable mark and the second optically detectable mark may be the same or different.

[0097] In this embodiment, referring to Figures 6 and 11, the base plate 100 is provided with a second cavity 120. The imaging mechanism 400 includes a first guide rail 430 disposed on the base plate 100, a support plate 440 disposed on the first guide rail 430, a third driving member 450 disposed below the base plate 100, and a connecting plate 460 with one end connected to the third driving member 450 and the other end passing through the second cavity 120 and connected to the support plate 440. Under the driving action of the third driving member 450, the connecting plate 460 drives the support plate 440 to move on the first guide rail 430. The camera 410 and the light source 420 are disposed on the support plate 440.

[0098] The third driving member 450 can drive the connecting plate 460 to move. If the third driving member 450 is a motor, the output shaft of the motor is connected to the connecting plate 460. When the motor is working, it drives the connecting plate 460 to move through the output shaft.

[0099] To save space, the third driving member 450 is located below the base plate 100.

[0100] A slider can be provided on the first guide rail 430, and the support plate 440 is provided on the slider. When the third driving member 450 drives the support plate 440 to move, the support plate 440 drives the slider to move on the first guide rail 430.

[0101] The third driving member 450 drives the connecting plate 460 to move, the connecting plate 460 drives the support plate 440 to move on the first guide rail 430, and the support plate 440 drives the camera 410 and the light source 420 to move, so that the camera 410 can take pictures of different positions of the electrophoresis chip 2000. Since each channel 2140 operates independently, the camera 410 does not need to wait for all channels 2140 to complete electrophoresis, and can take pictures of the ready channels 2140.

[0102] In this embodiment, the sample loading mechanism 600 includes a sample storage container 610 and a sample loading device for extracting samples from the sample storage container 610.A sample loading component is used to measure samples and load the samples to be tested into the flow channels 2140 of the electrophoresis chip 2000.

[0103] The sample holder 610 is disposed on the base plate 100 and includes a sample storage base and a multi-well plate disposed on the sample storage base, such as a 96-well plate.

[0104] The sample loading component moves to the corresponding position of the sample to be tested on the multi-well plate, extracts the sample to be tested, and then moves to the corresponding position of the electrophoresis chip 2000 to load the sample to be tested into the flow channels 2140 of the electrophoresis chip 2000. The sample loading component can be operated multiple times to load each sample to be tested into different flow channels 2140 of the electrophoresis chip 2000.

[0105] In some specific embodiments of this application, as shown in FIG2 and FIG7, the sample loading component includes: a sampler 621, a first driving component 622, a second driving component 623, and a third driving component 624.

[0106] The sampler 621 is disposed on the first driving component 622, as shown in FIG11, and includes a puncture needle 6211 and a sample dispensing needle 6212 disposed on the first driving component specification page 8 / 26 11 CN 121347633 A 622. The first driving component 622 drives the sampler 621 to move in a first direction (the z direction as shown in FIG2); the first driving component 622 is disposed on the second driving component 623, and the second driving component 623 drives the first driving component 622 and the sampler 621 to move in a second direction (the y direction as shown in FIG1); the second driving component 623 is disposed on the third driving component 624, and the third driving component 624 drives the second driving component 623, the first driving component 622 and the sampler 621 to move in a third direction (the x direction as shown in FIG1). Thus, the sampler 621 moves in the first direction, the second direction and the third direction, so that the sampler 621 can smoothly complete the sample dispensing work.

[0107] In some specific embodiments of this application, referring to FIG7, FIG11 and FIG12, the first drive assembly 622 includes a first moving plate 6221, a second guide rail 6222, a first synchronous belt 6223, a first driving wheel 6224, a first driven wheel 6225, and a fourth drive member 6226; the second drive assembly 623 includes a second moving plate 6231, a third guide rail 6232, a second synchronous belt 6233, a second driving wheel 6234, a second driven wheel 6235, and a fifth drive member 6236; and the third drive assembly 624 includes a fourth guide rail 6241, a third synchronous belt 6242, a third driving wheel 6243, a third driven wheel 6244, and a sixth drive member 6245.

[0108] Two fourth guide rails 6241 are provided, respectively on both sides of the base plate 100. The second moving plate 6231 is provided on the fourth guide rail 6241 and is fixedly connected to the third synchronous belt 6242. The third synchronous belt 6242 wraps around the third driving wheel 6243 and the third driven wheel 6242.A driving wheel 6244 is provided, and a third driving wheel 6243 and a third driven wheel 6244 are provided on the base plate 100. A sixth driving component 6245 drives and connects to the third driving wheel 6243. A third guide rail 6232 is provided on the second moving plate 6231, and a first moving plate 6221 is provided on the third guide rail 6232 and fixedly connected to the second synchronous belt 6233. The second synchronous belt 6233 is arranged around the second driving wheel 6234 and the second driven wheel 6235. The second driving wheel 6234 and the second driven wheel 6235 are arranged around the second driving wheel 6234 and the second driven wheel 6235. On the moving plate 6231, the fifth driving member 6236 drives and connects to the second driving wheel 6234; the second guide rail 6222 is set on the first moving plate 6221, the sampler 621 is set on the second guide rail 6222 and fixedly connected to the first synchronous belt 6223, the first synchronous belt 6223 is set around the first driving wheel 6224 and the first driven wheel 6225, the first driving wheel 6224 and the first driven wheel 6225 are set on the first moving plate 6221, and the fourth driving member 6226 drives and connects to the first driving wheel 6224.

[0109] The fourth driving member 6226 can drive the first driving wheel 6224 to rotate. If the fourth driving member 6226 is a motor, the output shaft of the motor is connected to the first driving wheel 6224, and when the motor is working, it drives the first driving wheel 6224 to rotate through the output shaft.

[0110] The fifth driving member 6236 can drive the second driving wheel 6234 to rotate. If the fifth driving component 6236 is a motor, the output shaft of the motor is connected to the second driving wheel 6234. When the motor is working, it drives the second driving wheel 6234 to rotate through the output shaft.

[0111] The sixth driving component 6245 can drive the third driving wheel 6243 to rotate. If the sixth driving component 6245 is a motor, the output shaft of the motor is connected to the third driving wheel 6243. When the motor is working, it drives the third driving wheel 6245 to rotate through the output shaft.

[0112] When the fourth driving component 6226 is working, it transmits power to the first driving wheel 6224. The first driving wheel 6224 rotates, driving the first synchronous belt 6223 and the first driven wheel 6225 to rotate. Since the sampler 621 is fixedly connected to the first synchronous belt 6223, the sampler 621 moves in the first direction.

[0113] When the fifth driving member 6236 is working, it transmits power to the second driving wheel 6234. The second driving wheel 6234 rotates, driving the second synchronous belt 6233 and the second driven wheel 6235 to rotate. Since the first moving plate 6221 is fixedly connected to the second synchronous belt 6233, the first moving plate 6221 moves in the second direction. The sampler 621 is set on the first moving plate 6221, thus enabling the sampler 621 to move in the second direction.

[0114] When the sixth driving member 6245 is working, it transmits power to the third driving wheel 6243. The third driving wheel 6243 rotates, driving the second driving wheel 6234 and the driven wheel 6235 to rotate.The third synchronous belt 6242 and the third driven wheel 6244 rotate. Since the second moving plate 6231 is fixedly connected to the third synchronous belt 6242, the second moving plate 6231 moves upward in the third direction. The first moving plate 6221 is set on the second moving plate 6231, which also enables the second moving plate 6231 and the sampler 621 to move upward in the third direction.

[0115] The fixed connection between the sampler 621 and the first synchronous belt 6223 can be achieved by providing at least one through hole on the first synchronous belt 6223. A fastener such as a screw passes through the through hole and extends into the sampler 621, thereby fixing the two together.

[0116] The fixed connection between the first moving plate 6221 and the second synchronous belt 6233 can be achieved by providing at least one through hole on the second synchronous belt 6233. A fastener such as a screw passes through the through hole and extends into the first moving plate 6221, thereby fixing the two together.

[0117] The second moving plate 6231 and the third synchronous belt 6242 can be fixedly connected by providing at least one through hole on the third synchronous belt 6242, and passing a fastener such as a screw through the through hole and into the second moving plate 6231 to achieve the fixation of the two.

[0118] A slider can be provided on the second guide rail 6222, and the sampler 621 is provided on the slider. When the fourth driving member 6226 drives the sampler 621 to move, the sampler 621 drives the slider to move on the second guide rail 6222.

[0119] A slider can be provided on the third guide rail 6232, and the first moving plate 6221 is provided on the slider. When the fifth driving member 6236 drives the first moving plate 6221 to move, the first moving plate 6221 drives the slider to move on the third guide rail 6232.

[0120] A slider can be provided on the fourth guide rail 6241, and the second moving plate 6231 is provided on the slider. When the sixth driving member 6245 drives the second moving plate 6241 to move, the second moving plate 6241 drives the slider to move on the fourth guide rail 6241.

[0121] In some specific embodiments of this application, referring to FIG7, the sample loading assembly also includes a nozzle holder 630 provided on the base plate 100. The nozzle holder 630 is provided with a plurality of first storage cavities for placing new nozzles and at least one second storage cavity for placing old nozzles. The second storage cavity is provided with a slot so that the old nozzle on the sampling needle 6212 can be removed and dropped into the second storage cavity.

[0122] The nozzle is a tip. After the sampling needle 6212 is fitted with the tip, it can extract the sample to be tested from the sample holder 610 and load the sample to be tested into the flow channel 2140 of the chip 2000.

[0123] The flow process of the sampler 621 is described in detail here. When the chip 2000 is in the analysis station, the sampler 621 moves toAbove the first storage chamber of the pipette tip holder 630, the sampler 621 moves downward, placing the pipette tip onto the sampling needle 6212. The sampler 621 moves upward, moving above the sample holder 610. The sampler 621 then moves downward, drawing the sample to be tested. The sampler 621 moves upward, moving above the electrophoresis chip 2000. At this point, the puncture needle 6211 is positioned above the flow channel 2140 of the electrophoresis chip 2000 to be sampled. The sampler 621 moves downward, puncturing the electrophoresis chip 2000 with the puncture needle 6211. The sampler 621 moves upward, moving slightly upward, with the sampling needle 6212 positioned above the punctured flow channel 2140 of the electrophoresis chip 2000. The sampler 621 then moves downward, loading the sample to be tested into the flow channel 2140. The sampler 621 moves upward and moves to the upper part of the second storage cavity of the tip holder 630. The sampler 621 moves downward and inserts the tip into the slot. The sampler 621 moves upward so that the tip is removed from the sample needle 6212 and falls into the second storage cavity. In other embodiments, the above steps can be interchanged. For example, the puncture can be performed first, and then the tip can be put on the sample needle 6212, followed by sampling, adding the sample, and removing the tip; or the tip can be put on the sample needle 6212 first, and then the puncture can be performed, followed by sampling, adding the sample, and removing the tip.

[0124] Please refer to Figures 13 to 19. The embodiments of this application provide an electrophoresis chip 2000, which can satisfy the requirement of performing electrophoresis operation while performing sample addition operation.

[0125] The electrophoresis chip 2000 includes a substrate 2100 and a cover plate 2200 covering the substrate 2100;

[0126] The substrate 2100 is provided with a plurality of flow channels 2140 for accommodating the sample to be tested, and each flow channel 2140 has a sample loading position provided with a sample loading groove 2160, the extension direction a of the sample loading groove 2160 is the same as the extension direction b of the corresponding flow channel 2140;

[0127] Each flow channel 2140 has an electrode assembly 2120 at both ends, and each flow channel 2140 is offset from the electrode contact end 2211 of the corresponding electrode assembly 2120, so that the extension direction of the sample loading groove 2160 does not intersect with the vertical center line direction of the electrode contact end 2211. Instruction manual, page 10 / 26, 13 CN 121347633 A

[0128] Wherein, the electrophoresis chip 2000 is placed vertically when in the analysis station, and the two electrode assemblies 2120 located at both ends of the flow channel 2140 are the positive electrode assembly and the negative electrode assembly, respectively. The probe of the electrophoresis mechanism 300 contacts the electrode assembly 2120, generating a voltage applied to the sample to be tested in the flow channel 2140, thereby realizing the electrophoretic separation of the sample to be tested. Specifically, after the chip fixing mechanism 200 fixes the electrophoresis chip 2000, the sample loading mechanism 600 loads multiple flow channels disposed in the electrophoresis chip 2000.Each channel 2140 in the electrophoresis chip 2000 is sequentially subjected to sample loading. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 within the electrophoresis chip 2000 that have completed sample loading, so that the test samples within the channels 2140 are separated by electrophoresis. The imaging mechanism 400 takes pictures of each channel 2140 that has completed electrophoresis to obtain sample imaging images corresponding to each channel 2140. The obtained sample imaging images are then analyzed to obtain information such as the fragment length, concentration, and integrity of the test samples. In this embodiment, by misaligning the channels 2140 with the electrode contact ends 2211 of the corresponding electrode assembly 2120 and ensuring that the extension direction of the sample loading groove 2160 does not intersect with the vertical centerline direction of the electrode contact end 2211, spatial interference is eliminated, and the parallel execution of the sample loading and electrophoresis operations is achieved, thereby improving the detection efficiency of the electrophoresis chip 2000.

[0129] The substrate 2100 and the cover plate 2200 can be made of inorganic insulating materials, organic insulating materials, polymer insulating materials, composite materials, or a combination of materials. The substrate 2100 is preferably made of polypropylene. Polypropylene has good light transmittance and its surface will not dissociate ions in an aqueous environment. It can also minimize electroosmosis without surface treatment, thereby avoiding affecting the electrophoretic separation process of the sample to be tested.

[0130] In this embodiment, the substrate 2100 can be made of transparent material, which can ensure that when the imaging mechanism 400 takes pictures of the sample, it can obtain clear pictures smoothly and will not cause the pictures to be blurry due to the substrate 2100.

[0131] In this embodiment, as shown in Figures 13-16, the substrate 2100 of the electrophoresis chip 2000 has a first surface α and a second surface β opposite to the first surface α. The electrode hole 2110 penetrates the first surface α and the second surface β, and the flow channel 2140 is disposed on the first surface α of the substrate 2100.

[0132] As shown in Figures 17-19, the cover plate 2200 has a third surface γ and a fourth surface δ opposite to the third surface γ. The fourth surface δ of the cover plate 2200 covers the first surface α of the substrate 2100, and the printed electrode layer 2210 is disposed on the fourth surface δ of the cover plate 2200.

[0133] During the testing process, the electrophoresis chip 2000 is placed vertically. The probes of the 16 channels of the electrophoresis mechanism 300 will be simultaneously inserted horizontally from the side electrode hole 2110 of the substrate 2100, passing through the second surface β of the substrate 2100 and the first surface α of the substrate 2100 in sequence, and finally contacting the motor contact end 2211 on the printed electrode layer 2210 on the fourth surface δ of the cover plate 2200.

[0134] In this embodiment, as shown in Figures 14, 15, 17, and 18, the electrode assembly 2120 includes an electrode hole 2110 disposed on the substrate 2100 and a printed electrode layer 2210 disposed on the cover plate 2200. Referring to Figures 14 and 15, on the cover plate...When 2200 is applied to the substrate 2100, the printed electrode layer 2210 projected onto the first surface α of the substrate 2100 is shown by dashed lines. The electrode contact ends 2211 of the printed electrode layer 2210 correspond to the electrode holes 2110, and each flow channel 2140 is offset from the electrode contact ends 2211 and electrode holes 2110 of the corresponding electrode assembly 2120. The electrode assembly 2120 adopts a structure in which the electrode holes 2110 provided on the substrate 2100 are combined with the printed electrode layer 2210 provided on the cover plate 2200, and the electrode contact ends 2211 of the printed electrode layer 2210 correspond to the electrode holes 2110, while each flow channel 2140 is offset from the electrode contact ends 2211 and electrode holes 2110 of the corresponding electrode assembly 2120. This design not only eliminates spatial interference and ensures that the sample loading operation and electrophoresis operation can be performed in parallel to improve detection efficiency, but also controls the consistency of the testing process conditions for each sample through the cooperation of the electrode hole 2110 and the printed electrode layer 2210 to ensure repeatability.

[0135] The surface of the substrate 2100 is generally provided with grooves and protrusions. The printed circuit solution on the substrate 2100 is prone to flow into the microstructure, causing short circuits or broken lines. In order to ensure that the electrode is formed in one step and the electrode is reliable, the graphene printed electrode needs to be printed on the cover plate 2200. Only the electrode hole 2110 needs to be opened on the substrate 2100, without involving the electrode crossing the bonding surface. Specification 11 / 26 pages 14 CN 121347633 A

[0136] In this embodiment, the printed electrode layer 2210 is preferably a graphene printed electrode, but other inert metal electrodes, such as copper electrodes, platinum electrodes, gold electrodes, etc. can also be used. The structure and composition of the printed electrode layer 2210 are shown in Figures 17 and 18.

[0137] In some specific embodiments of this application, for the electrode assembly 2120 formed after the cover plate 2200 is placed on the substrate 2100, as shown in Figures 14 and 17, the printed electrode layer 2210 includes an electrode contact end 2211, a contact end 2212, and a connecting portion 2213 for connecting the electrode contact end 2211 and the contact end 2212. The connecting portion 2213 of the printed electrode layer 2210 includes a horizontal portion 22131 and a vertical portion 22132. One end of the horizontal portion 22131 is connected to the contact end 2212, and the other end of the horizontal portion 22131 is connected to one end of the vertical portion 22132. The other end of the vertical portion 22132 is connected to the electrode contact end 2211. The horizontal portion 22131 of the connecting portion 2213 spans the buffer chamber 2150 of the flow channel 2140, and the width of the horizontal portion 22131 is greater than 1.5 mm. To reduce the current density on the electrode surface of the electrophoresis chip 2000, thereby mitigating the risk of power outage caused by localized aggregation of electrolytic bubbles, and to provide sufficient space for sample aggregation after sample addition while ensuring effective contact between the graphene-printed electrode and the sample after electrolysis, this design aims to achieve the desired effect.The buffer chamber 2150 has an upper edge of horizontal portion 22131 that is more than 0.5 mm from the top of the buffer chamber 2150, and a lower edge of horizontal portion 22131 that is more than 1.5 mm from the narrowest point of the buffer chamber 2150.

[0138] In some other embodiments of this application, for the electrode assembly 2120 formed after the cover plate 2200 is placed on the substrate 2100, as shown in Figures 15 and 18, the printed electrode layer 2210 includes an electrode contact end 2211, a contact end 2212, and a connecting portion 2213 for connecting the electrode contact end 2211 and the contact end 2212. The connecting portion 2213 of the printed electrode layer 2210 includes a horizontal portion 22131 and a bent portion 22133. One end of the horizontal portion 22131 is connected to the contact end 2212, one end of the bent portion 22133 is connected to the middle position of the horizontal portion 22131, and the other end of the bent portion 22133 is connected to the electrode contact end 2211.

[0139] In this embodiment, in order to achieve reliable substrate thermal bonding, the minimum bonding distance between the electrode hole 2110 and the flow channel 2140 of the electrophoretic chip 2000 is greater than 1.0 mm, so that the electrode hole 2110 and the flow channel 2140 are well sealed. Among them, the minimum bonding distance refers to the minimum allowable distance between the centers of two adjacent bonding points, such as pads, leads or bumps, in chip packaging or circuit board manufacturing. It is a key parameter to ensure the reliability of the bonding process and avoid short circuits or mechanical interference.

[0140] In this embodiment, Figures 14 and 15 show that the diameter of the electrode contact end 2211 is larger than the diameter of the electrode hole 2110. For example, taking the substrate 2100 as having 16 flow channels 2140 for accommodating the sample to be tested as an example, since the first surface α of the substrate 2100 has 16 flow channels 2140, and both ends of the flow channels 2140 are printed with printed electrode layers 2210, considering the positioning accuracy requirements during hot pressing, the diameter of the electrode contact end 2211 of the printed electrode layer 2210 is 0.2 mm larger than the diameter of the electrode hole 2110 on the substrate 2100, so that the electrode contact end 2211 can fully fill the electrode hole 2110 and realize the contact between the metal electrode (electrode hole 2110) and the graphene electrode (electrode contact end 2211).

[0141] In this embodiment, as shown in Figures 14 and 15, the contact end 2212 of the printed electrode layer 2210 of the electrophoresis chip 2000 corresponds to the end 2130 of the corresponding flow channel 2140, and the connecting portion 2213 of the printed electrode layer 2210 bypasses the side or end 2130 of the corresponding flow channel 2140 so that the electrode contact end 2211 is misaligned with the corresponding flow channel 2140. This design eliminates spatial interference, enables parallel sample loading and electrophoresis operations to improve detection efficiency, and further optimizes the layout of the electrodes and flow channels. The bypass design can make full use of the spatial layout so that the electrophoresis and puncture sample loading processes in each flow channel 2140 can be performed independently.

[0142] During sample testing, when the electrophoresis chip 2000 is placed vertically, the probe of the electrophoresis mechanism 300, which performs electrophoresis on each channel 2140, is horizontally inserted into the electrode hole 2110 to contact the electrode contact end 2211 of the printed electrode layer 2210, generating a voltage applied to the sample to be tested in the channel 2140, thereby achieving electrophoretic separation of the sample to be tested.

[0143] In some specific embodiments of this application, as shown in Figures 13, 14, and 15, the connection portion 2213 of the corresponding printed electrode layer 2210 of the non-edge channel 2140 of the electrophoresis chip 2000 is arranged around the side or end 2130 of the corresponding channel 2140, so that the corresponding electrode contact end 2211 is located in the interval area between adjacent channels 2140. For edge channels 2140, the connecting portion 2213 of the corresponding printed electrode layer 2210 is arranged around the side or end 2130 of the corresponding channel 2140, so that the electrode contact end 2211 corresponding to page 12 / 26 of the specification 15 CN 121347633 A is located on the side of the corresponding channel 2140 away from the adjacent channel 2140, or the electrode contact end 2211 corresponding to one edge channel 2140 is located on the side of the corresponding channel 2140 away from the adjacent channel 2140, and the electrode contact end 2211 corresponding to the other edge channel 2140 is located in the gap area between the other edge channel 2140 and the adjacent channel 2140. This design eliminates spatial interference, enables parallel sample loading and electrophoresis operations to improve detection efficiency, and further optimizes the layout of the electrode contact ends. In addition, by reasonably placing the electrode contact end 2211 in the interval area or away from the adjacent flow channel, the internal space of the electrophoresis chip 2000 can be fully utilized, and the interference of the electrode contact end 2211 to the sample to be tested in the flow channel 2140 can be avoided, thereby improving the electrophoretic separation effect. At the same time, this layout also facilitates the accurate contact between the probe of the electrophoresis mechanism 300 and the electrode contact end 2211, thereby improving the stability and reliability of the operation.

[0144] In this embodiment, when the electrophoresis chip 2000 is sample tested, the sample dispensing needle 6212 and / or puncture needle 6211 of the sample dispensing mechanism 600, which is used to dispense samples to each flow channel 2140, are vertically inserted into the sample dispensing groove 2160. The vertical movement path of the sample dispensing needle 6212 and / or puncture needle 6211 does not overlap with the horizontal movement path of the probe in the spatial projection.

[0145] The electrophoresis chip 2000 in this application is structured such that the electrode hole 2110 is offset from the test flow channel 2140. While one or more flow channels 2140 are undergoing electrophoresis, the remaining available flow channels 2140 can still be used for sample loading, sample loading waiting, and post-sample loading electrophoresis, saving time. Because the probe passing through the electrode hole 2110 and the sample loading needle 6212 and / or puncture needle 6211 inserted into the sample loading slot 2160 are avoided, the puncture, sample loading, and electrophoresis processes among the 16 flow channels 2140 are independent of each other, enabling parallel sample loading operations.The electrophoresis operation effectively shortens the total time required for sample imaging. By optimizing the time, efficiency is improved. Furthermore, the intervals between each test step for each sample are kept consistent to ensure repeatability.

[0146] In some embodiments, the analysis device 10 can also be applied to the electrophoresis chip 3000 shown in Figures 20 to 22.

[0147] The electrophoresis chip 3000 includes a cover plate 3200 disposed on a substrate 3100. Multiple flow channels 3110 are disposed on the substrate 3100, and electrode assemblies 3120 are disposed at both ends of each flow channel 3110. Since there are multiple flow channels, and each flow channel 3110 has electrode assemblies 3120 at both ends, each flow channel 3110 can be loaded with a sample to be tested. Therefore, after each flow channel 3110 is loaded with a sample to be tested, multiple samples can be simultaneously separated by electrophoresis and imaged, greatly improving analysis efficiency. The substrate 3100 has a groove 3130 corresponding to the sample loading position of each flow channel 3110. The recess direction of the groove 3130 is the same as the setting direction of the corresponding flow channel 3110. As shown in Figure 21, the d direction is the recess direction of the groove 3130, the c direction is the setting direction of the flow channel 3110, and the setting direction of the flow channel 3110 is the length direction of the flow channel 3110.

[0148] The substrate 3100 can be made of inorganic insulating material, organic insulating material, polymer insulating material, composite material, or a combination of materials. The substrate 3100 is preferably made of polypropylene material. Polypropylene material has good light transmittance, and its surface will not dissociate ions in an aqueous environment. It can also avoid electroosmosis without surface treatment, thereby avoiding affecting the electrophoretic separation process of the sample to be analyzed.

[0149] The electrode assembly 3120 includes a contact end 3121, a contact end 3122, and a connecting end 3123 connecting the contact end 3121 and the contact end 3122. The contact end 3122 contacts the corresponding flow channel 3110, and the contact end 3121 is disposed on a protrusion 3140 formed between two adjacent grooves 3130.

[0150] The probe of the electrophoresis mechanism 300 contacts the contact end 3121, and the contact end 3121 transmits electricity to the contact end 3122 through the connecting end 3123, thereby causing the electrode assembly 3120 to apply a voltage to the sample to be tested in the flow channel 3110.

[0151] The contact end 3121 is disposed on the boss 3140 formed between two adjacent grooves 3130. In the substrate 3100, the structural strength and rigidity of the boss 3140 are greater than those of the grooves 3130, thereby preventing deformation or damage to the substrate 3100 when the probe contacts the contact end 3121 and applies force to the contact end 3121.

[0152] The cover plate 3200 is provided with a piercing hole 3210 corresponding to the contact end 3121. By providing the piercing hole 3210, it is possible to...This allows the probe to pass through the puncture hole 3210 and contact the electrode assembly 3120, thereby smoothly applying voltage to the sample to be tested. The puncture hole 3210 also plays a certain positioning role, preventing the probe from moving to the wrong position.

[0153] The cover plate 3200 can be made of inorganic insulating material, organic insulating material, polymer insulating material, composite material, or a combination of materials. The cover plate 3200 is preferably made of polypropylene. Polypropylene has good light transmittance, and its surface will not dissociate ions in an aqueous environment. Without surface treatment, it can also avoid electroosmosis, thereby avoiding affecting the electrophoretic separation process of the sample to be tested.

[0154] For example, the flow channel 3110 includes a middle channel 3112 and a first channel 3111 and a second channel 3113 disposed at both ends of the middle channel 3112. The centerline of the middle channel 3112 is straight, and the first channel 3111 and the second channel 3113 are disposed at both ends of the centerline of the middle channel 3112. The middle channel 3112 is filled with gel and buffer solution. The electrode assembly 3120 is disposed on the substrate 3100, as shown in Figure 22. The contact end 3121 is disposed on the substrate 3100. The connection end 3123 and the contact end 3122 extend to the first channel 3111 or the second channel 3113. The contact end 3122 contacts the first channel 3111 or the second channel 3113.

[0155] If the first channel 3111 contacts the negative electrode assembly 3120 and the second channel 3113 contacts the positive electrode assembly 3120, then the first channel 3111 is the liquid inlet channel, and the sample addition position is set on the liquid inlet channel. The second channel 3113 is the liquid storage channel, and the sample to be tested is loaded into the first channel 3111. When the probe of the electrophoresis mechanism 300 contacts the electrode assembly 3120, generating a voltage applied to the sample in the first channel 3111, the sample moves from the first channel 3111 towards the second channel 3113 under the influence of the electric field. The gel, with its porous structure, can serve as a sieving medium, while the buffer solution maintains the degree of dissociation, thereby achieving differential separation of sample fragments of different lengths. These fragments form bands within the flow channel, completing the electrophoretic separation process. If the first channel 3111 contacts the positive electrode assembly 3120 and the second channel 3113 contacts the negative electrode assembly 3120, then the first channel 3111 becomes a reservoir channel, and the second channel 3113 becomes an inlet channel, loading the sample into the second channel 3113. When the probe of the electrophoresis apparatus 300 contacts the electrode assembly 3120, generating a voltage applied to the sample in the second channel 3113, the sample moves from the second channel 3113 towards the first channel 3111 under the influence of the electric field. The gel has a porous structure and can be used as a sieving medium, while the buffer solution maintains the degree of dissociation, thus enabling the processing of sample lengths.Differential separation of fragments: different lengths of sample fragments to be tested form strips in the flow channel, completing the electrophoretic separation process.

[0156] Please continue to refer to Figure 1, which is a schematic flowchart of the sample imaging method provided in the embodiment of this application. This sample imaging method can be applied to the analysis device 10 shown in Figures 2 to 12, and to the electrophoresis chip 2000 shown in Figures 13 to 19, the electrophoresis chip 2000 having multiple channels 2140 for accommodating the sample to be tested; or to the electrophoresis chip 3000 shown in Figures 20 to 22, the electrophoresis chip 3000 having multiple channels 3110 for accommodating the sample to be tested. The method may include the following steps:

[0157] Step 11, sequentially perform sample loading operation on each channel in the channels, and perform electrophoresis operation on the channels after the sample loading operation is completed, so that the sample to be tested in the channels is separated by electrophoresis, wherein, after the sample loading operation is completed in the (N-1)th channel, the sample loading operation is performed in the Nth channel, and the electrophoresis operation is performed in the (N-1)th channel, and after the sample loading operation is completed in the Nth channel, the electrophoresis operation is performed in the Nth channel, 1 < N ≤ the total number of channels, N is a natural number;

[0158] Step 12: Take a picture of each channel that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel.

[0159] For example, taking the electrophoresis chip 2000 shown in Figures 2 to 13 as an example, the parallel electrophoresis method provided in this application includes three processes: sample loading operation, electrophoresis operation, and imaging operation. Each process will be described in detail below.

[0160] Among them, the sample loading operation is the starting point of the entire parallel electrophoresis process and is executed by the sample loading mechanism 600. The sample loading mechanism 600 has high-precision positioning and quantitative sample loading capabilities, which can ensure that the sample to be tested is accurately added to each channel 2140. In actual operation, the sample loading mechanism 600 can sequentially perform sample loading operations on each channel 2140 in the channel 2140 according to a preset order. For example, starting from the first channel 2140, an appropriate amount of sample to be tested is injected into the channel specification page 14 / 26 17 CN 121347633 A 2140. During the sample addition process, the sample addition mechanism 600 precisely controls the addition speed and amount to avoid sample overflow or insufficient addition. Simultaneously, to ensure uniform distribution of the sample within the flow channel 2140, the sample addition mechanism 600 may employ specific addition methods, such as slow dripping or pulsed addition.

[0161] Electrophoresis is a crucial step in separating the sample within the flow channel 2140, and is performed by the electrophoresis mechanism 300. The electrophoresis mechanism 300 can apply a suitable electric field to both ends of the flow channel 2140, driving different components in the sample to migrate at different speeds under the influence of the electric field, thereby achieving separation. The flow channel 2140, after the sample addition operation, is then processed by the electrophoresis mechanism 300.Electrophoresis is performed to separate the test samples within the flow channels 2140. This embodiment cleverly utilizes a time difference to achieve parallel processing of sample addition and electrophoresis. Specifically, after the sample addition operation is completed in the (N-1)th flow channel 2140, the sample addition mechanism 600 does not wait for the electrophoresis operation in that flow channel 2140 to complete, but immediately continues the sample addition operation in the Nth flow channel 2140. Simultaneously, the electrophoresis mechanism 300 quickly applies an electric field to the (N-1)th flow channel 2140 that has already completed sample addition to perform electrophoresis, causing the test samples within that (N-1)th flow channel 2140 to begin electrophoretic separation. Furthermore, after the sample addition operation is completed in the Nth flow channel 2140, the electrophoresis mechanism 300 also quickly applies an electric field to the Nth flow channel 2140 to perform electrophoretic separation, causing the test samples within that Nth flow channel 2140 to begin electrophoretic separation. 1 < N ≤ the total number of flow channels 2140, where N is a natural number.

[0162] Imaging is a crucial step in obtaining the electrophoretic separation results of the samples, and is performed by the imaging mechanism 400. The imaging mechanism 400 possesses high-resolution imaging capabilities, enabling it to clearly photograph each channel 2140 that has completed the electrophoresis operation, thereby acquiring sample imaging images corresponding to each channel 2140.

[0163] For example, the imaging mechanism 400 sequentially images each channel 2140 that has completed the electrophoresis operation. The imaging mechanism 400 precisely controls parameters such as the shooting angle, focal length, and exposure time to ensure that the acquired sample imaging images are of high quality and rich in information. For example, by adjusting the shooting angle, the influence of reflections and shadows on image quality can be avoided; by optimizing the focal length, sample details in the image can be made clearer; by reasonably setting the exposure time, images that are too bright or too dark can be prevented, ensuring that all components of the sample under test are clearly visible.

[0164] This embodiment of the application achieves a complete parallel operation process by performing sample loading and electrophoresis operations in parallel, giving full play to the advantages of the electrophoresis chip 2000 channels 2140. The close cooperation between each stage from sample loading, electrophoresis to imaging can effectively shorten the total time required for sample imaging and improve the efficiency and continuity of the sample imaging process. In the sample loading stage, by loading samples sequentially and quickly turning to the next channel 2140, the waiting time in the sample loading process is reduced; in the electrophoresis stage, the electrophoresis operations of different channels 2140 are performed in parallel, making full use of the working time of the electrophoresis mechanism 300 and improving the equipment utilization rate; in the imaging stage, each channel 2140 is imaged in an orderly manner, ensuring that the acquired image is complete and accurate.

[0165] In some embodiments, before performing the sample loading operation sequentially on each of the plurality of channels, the method further includes: pre-processing the electrophoresis chip, the pre-processing including at least one of cleaning, drying and dust removal.

[0166] For example, before sequentially performing sample loading operations on each of the plurality of channels 2140, the electrophoresis chip is...2000 is pre-treated, and the pre-treatment includes at least one of cleaning, drying and dust removal.

[0167] For example, pre-cleaning the electrophoresis chip 2000 can remove contaminants on the chip surface and avoid interfering with electrophoresis; drying the electrophoresis chip 2000 can prevent residual liquid from diluting the sample; dust removal is performed on the surface of the pre-cleaned electrophoresis chip 2000 to avoid impurities affecting image quality during photography.

[0168] In some embodiments, before the sample loading operation is performed sequentially on each of the plurality of channels, the method further includes: calibrating the sample loading mechanism used for the sample loading operation, the calibration operation including at least one of vertical positioning calibration, horizontal position calibration and sample loading volume calibration.

[0169] For example, before the sample loading operation is performed sequentially on each of the plurality of channels 2140, the sample loading mechanism 600 used for the sample loading operation is calibrated, the calibration operation including at least one of vertical positioning calibration, horizontal position calibration and sample loading volume calibration.

[0170] For example, vertical positioning calibration ensures that the puncture needle 6211 is aligned with the flow channel 2140. If the puncture needle deviates in the vertical direction, it may lead to inaccurate puncture during sample addition, or even damage to the flow channel or chip, thus affecting the entire experiment. Therefore, vertical positioning calibration ensures accurate vertical positioning of the puncture needle, providing a stable basis for subsequent sample addition operations.

[0171] For example, horizontal position calibration ensures that the sampler 621 moves without deviation, ensuring that each flow channel 2140 receives sample addition at the accurate position. If the sampler 621 deviates in the horizontal direction, it may lead to incorrect sample addition or omission, thus affecting the accuracy and reliability of the experimental results. Therefore, horizontal position calibration eliminates this potential risk and ensures the accuracy of the sample addition operation.

[0172] For example, sample volume calibration can reduce sample volume errors. If there is a deviation in the sample volume, it may lead to inconsistent sample concentrations in the experiment, thus affecting the comparison and analysis of experimental results. Therefore, by calibrating the sample volume, it can be ensured that the sample volume received by each channel is accurate, providing a strong guarantee for the smooth progress of the experiment.

[0173] In some embodiments, each channel in the electrophoresis chip is offset from the electrode contact end corresponding to each channel; when the probe of the electrophoresis mechanism for performing electrophoresis contacts the electrode contact end corresponding to each channel in the electrophoresis chip, the moving path of the sample dispensing needle of the sample dispensing mechanism for performing sample dispensing does not overlap with the moving path of the probe in spatial projection.

[0174] As shown in Figures 14 and 15, each channel 2140 in the electrophoresis chip 2000 and the electrode corresponding to each channel 2140 are...The contact ends 2211 are staggered; when the probe of the electrophoresis mechanism 300 used for electrophoresis contactes the electrode contact end 2211 corresponding to each channel 2140 in the electrophoresis chip 2000, the movement path of the sample dispensing needle 6212 of the sample dispensing mechanism 600 used for sample dispensing does not overlap with the movement path of the probe in spatial projection. The staggered arrangement of the channel 2140 and the electrode contact ends 2211 structurally avoids collisions that may occur due to overlapping positions of the sample dispensing needle and the probe during operation. For example, in actual operation, when the sample dispensing needle 6212 adds the sample to the channel 2140, the probe can independently contact the electrode contact end 2211 to perform electrophoresis-related operations without interference, eliminating spatial interference and enabling parallel processing of the sample dispensing and electrophoresis operations to improve detection efficiency.

[0175] In some embodiments, the sample application mechanism further includes a puncture needle; when the probe contacts the electrode contact end corresponding to each flow channel in the electrophoresis chip, the movement path of the puncture needle and the movement path of the probe do not overlap in spatial projection.

[0176] As shown in FIG11, the sample application mechanism 600 further includes a puncture needle 6212. Before the sample application needle 6212 performs the sample application operation, the puncture needle 6212 first punctures into the flow channel 2140, and the puncture position of the flow channel 2140 forms a hole. Then the sample application needle 6212 passes through the hole to add the sample to be tested into the flow channel 2140. When the probe contacts the electrode contact end 2211 corresponding to each flow channel 2140 in the electrophoresis chip 2000, the movement path of the puncture needle 6211 and the movement path of the probe do not overlap in spatial projection. The probe moves horizontally, and the path of the puncture needle 6211 moves vertically. The path is separated under the misalignment design, which ensures the stability of concurrent operation.

[0177] In some embodiments, the sequential sample addition operation for each of the plurality of channels includes: sequentially taking a pipette tip, aspirating a sample, and loading a sample for each of the plurality of channels.

[0178] For example, sequentially adding a sample to each of the plurality of channels 2140 includes: sequentially taking a pipette tip, aspirating a sample, and loading a sample for each of the plurality of channels 2140. Sequentially taking a pipette tip, aspirating a sample, and loading a sample avoids cross-contamination of samples and improves experimental safety. This process is precisely controlled by an automated sample addition mechanism 600 to ensure consistent sample volume in each channel 2140, thereby improving experimental accuracy. The time consumed for adding a sample to each channel 2140 remains essentially consistent, further ensuring the reliability of the comparison results. In some embodiments, the sampling mechanism for performing the sampling operation includes a puncture needle and a sampling needle. The operation of sequentially taking the pipette tip, aspirating the sample, and loading the sample into each of the plurality of flow channels includes: controlling the puncture.A puncture operation is performed on the target flow channel; after the puncture operation is completed, the sampling needle is controlled to sequentially perform the operations of picking up the pipette tip, aspirating the sample, and loading the sample, thereby loading the sample to be tested into the punctured target flow channel.

[0180] For example, the sampling mechanism 600 for performing the sampling operation includes a puncture needle 6211 and a sampling needle 6212. The pipette tip is picked up, the sample is aspirated, and the sample is loaded into each of the multiple flow channels 2140 in sequence. Sampling requires the puncture needle 6211 to pass through the sampling groove 2160. Therefore, in this embodiment, the sampling operation includes: controlling the puncture needle 6211 to puncture the target flow channel 2140; after the puncture operation is completed, controlling the sampling needle 6212 to sequentially perform the operations of picking up the pipette tip, aspirating the sample, and loading the sample, thereby loading the sample into the punctured target flow channel 2140.

[0181] To minimize spillage or contamination of the sample in the electrophoresis chip 2000 (or electrophoresis chip 3000), the sample loading area of ​​the electrophoresis chip 2000 (or electrophoresis chip 3000) typically has a certain thickness. Due to this thickness, the puncture needle needs to penetrate a distance of this thickness, resulting in low sample loading efficiency. Furthermore, this thickness has a certain rigidity, and if the puncture needle deviates from its direction during the puncture process, it may not be able to accurately puncture into the flow channel (flow channel 2140 on electrophoresis chip 2000, or flow channel 3110 on electrophoresis chip 3000). This application addresses this by providing a sample loading groove 2160 (electrophoresis chip 2000) and a recess 3130 (electrophoresis chip 3000) at the sample loading area of ​​each flow channel, effectively reducing the puncture distance of the puncture needle and thus greatly improving the efficiency and reliability of sample loading. Typically, the orifice diameter of the puncture needle is larger than that of the sample loading needle, ensuring smooth sample loading.

[0182] In some embodiments, the sample loading mechanism for performing the sample loading operation includes a puncture needle and a sample loading needle. The operation of sequentially taking the pipette tip, aspirating the sample, and loading the sample in each of the plurality of flow channels includes: controlling the sample loading needle to sequentially take the pipette tip and aspirate the sample; controlling the puncture needle to perform a puncture operation in the target flow channel; and after the puncture operation is completed, controlling the sample loading needle to load the sample to be tested into the target flow channel.

[0183] For example, the sample loading mechanism 600 for performing sample loading operations includes a puncture needle 6211 and a sample loading needle 6212. It sequentially performs the operations of picking up the pipette tip, aspirating the sample, and loading the sample into each of the multiple flow channels 2140. Sample loading requires the puncture needle 6211 to pass through the sample loading groove 2160. The order of the puncture actions can be different. Therefore, in this embodiment, the sample loading operation includes controlling the sample loading needle 6212 to sequentially pick up the pipette tip and aspirate the sample; controlling the puncture needle 6211 to puncture the target flow channel 2140; and controlling the sample loading needle 6212 to load the sample into the target flow channel 2140 after the puncture operation is completed. This method of first aspirating the sample and then puncturing...The operation sequence can eliminate sample liquid level fluctuations caused by puncture vibration, making it suitable for micro-sample operations and further reducing the risk of wasting the sample to be tested.

[0184] In some embodiments, for non-edge channels, the corresponding electrode contact end is located in the interval region between adjacent channels, and for edge channels, the corresponding electrode contact end is located on the side of the corresponding channel away from the adjacent channel, or the electrode contact end corresponding to one edge channel is located on the side of the corresponding channel away from the adjacent channel, and the electrode contact end corresponding to another edge channel is located in the interval region between the other edge channel and the adjacent channel, so that each channel in the electrophoresis chip is misaligned with the electrode contact end corresponding to each channel.

[0185] As shown in Figures 14 and 15, for non-edge channels 2140, the corresponding electrode contact end 2211 is located in the interval region between adjacent channels 2140. For edge channels 2140, the corresponding electrode contact end 2211 is located on the side of the corresponding channel 2140 away from the adjacent channel 2140. Alternatively, the electrode contact end 2211 of one edge channel 2140 is located on the side of the corresponding channel 2140 away from the adjacent channel 2140, and the electrode contact end 2211 of the other edge channel 2140 is located in the interval region between the other edge channel 2140 and the adjacent channel 2140, so that each channel 2140 in the electrophoresis chip 2000 and the electrode contact end 2211 corresponding to each channel 2140 are misaligned. This design eliminates spatial interference, enabling parallel sample loading and electrophoresis operations to improve efficiency (see page 17 / 26 of the instruction manual, 20 CN 121347633 A), and further optimizes the layout of the electrode contact ends 2211. Furthermore, placing the electrode contact ends 2211 appropriately in the intervening area or away from adjacent flow channels fully utilizes the internal space of the electrophoresis chip 2000, avoids interference from the electrode contact ends 2211 on the sample to be tested within the flow channel 2140, ensures smooth sample flow and uniform electric field distribution during electrophoresis, and improves electrophoretic separation. Simultaneously, this layout also facilitates accurate contact between the probes of the electrophoresis mechanism 300 and the electrode contact ends 2211, improving operational stability and reliability.

[0186] In some embodiments, the electrophoresis mechanism includes M sets of probes corresponding to the total number of channels. Each set of probes includes a positive probe and a negative probe. The electrophoresis operation on the channels after sample loading includes: before the sample loading operation is completed in the first channel, controlling all probes of the electrophoresis mechanism to simultaneously contact the electrode contact end of the corresponding channel; after the sample loading operation is completed in the (N-1)th channel, controlling the probes corresponding to the (N-1)th channel to be energized to perform electrophoresis on the (N-1)th channel; after the sample loading operation is completed in the Nth channel, controlling the probes corresponding to the Nth channel to be energized to perform electrophoresis on the Nth channel.

[0187] For example, the electrophoresis mechanism 300 is applicable to the electrophoresis chip 2000 shown in Figures 13 to 19. The electrophoresis mechanism 300 includes M probe groups corresponding to the total number of channels 2140, where M≤N. Each probe group includes a positive probe and a negative probe. The electrophoresis operation is performed on the channels 2140 after the sample loading operation is completed. This includes: before the sample loading operation is completed in the first channel 2140, controlling all probe groups of the electrophoresis mechanism 300 to simultaneously contact the electrode contact end 2211 of the corresponding channel 2140; after the sample loading operation is completed in the (N-1)th channel 2140, controlling the probe group corresponding to the (N-1)th channel 2140 to be energized to perform the electrophoresis operation on the (N-1)th channel 2140; after the sample loading operation is completed in the Nth channel 2140, controlling the probe group corresponding to the Nth channel 2140 to be energized to perform the electrophoresis operation on the Nth channel 2140. After sample loading is completed in each channel 2140, only the corresponding probe group (positive probe and negative probe) contacts the electrode contact end 2211 of that channel 2140 and is energized, achieving precise one-to-one control and avoiding unified control of all probe groups, thus improving the accuracy and flexibility of the electrophoresis process. In the process, all probe groups are synchronously contacted with the corresponding electrode contact end 2211 before sample loading is completed in the first channel 2140, avoiding the time required for each channel 2140 to move the probe individually before electrophoresis, thus improving testing efficiency. The sample loading-electrophoresis interval for each sample is consistent, eliminating testing differences caused by timing deviations.

[0188] For example, the electrophoresis chip 2000 is provided with 16 channels 2140 for accommodating the sample to be tested. For example, M is 16. The electrophoresis mechanism 300 can have two rows of probes, with 16 probes in each row. The probe groups consisting of two corresponding probes in each row constitute the positive probe and the negative probe. The 32 probes are fixed on the electrophoresis fixing plate 310. Before the electrophoresis operation of the first channel, all probes of the electrophoresis mechanism 300 simultaneously contact the electrode contact terminal 2211 but are not energized. After the sample loading operation of each channel 2140 is completed, the probe group corresponding to the channel 2140 where the sample loading operation is completed is energized to perform the electrophoresis operation.

[0189] In some embodiments, the method further includes: after the Nth channel completes the electrophoresis operation, controlling the probe group corresponding to the Nth channel to be de-energized; for the remaining channels that have not completed the sample loading operation, maintaining the physical contact between the corresponding probe group and the electrode contact end, but keeping them de-energized until the channel completes the sample loading operation and is ready for electrophoresis, then controlling the corresponding probe group to be energized.

[0190] For example, after the Nth channel 2140 completes the electrophoresis operation, controlling the probe group corresponding to the Nth channel 2140 to be de-energized. Timely de-energization after electrophoresis avoids the current of the completed channel 2140 interfering with the electric field of adjacent channels, reducing corrosion and energy consumption caused by excessive probe working time. For the remaining channels 2140 that have not completed the sample loading operation, maintaining the physical contact between the corresponding probe group and the electrode contact end, but keeping them de-energized, until the channel completes the sample loading operation and is ready for electrophoresis, then controlling the corresponding probe group to be energized.Physical contact with electrode contact 2211 is maintained, but power is off until the flow channel 2140 completes the sample loading operation and is ready for electrophoresis, at which point the corresponding probe group is powered on. The probe group of the unready flow channel 2140 remains in physical contact but is powered off. When the sample loading needle accidentally contacts the electrode contact, power-off protection is formed, further reducing the risk. Specification 18 / 26 pages 21 CN 121347633 A

[0191] In some embodiments, the electrophoresis mechanism includes M groups of probes corresponding to the total number of flow channels. Each group of probes includes a positive probe and a negative probe. The electrophoresis operation on the flow channel that has completed the sample loading operation includes: before the first flow channel completes the sample loading operation, controlling all probe groups of the electrophoresis mechanism to simultaneously contact the electrode contact of the corresponding flow channel and controlling all probe groups to be powered on.

[0192] For example, the electrophoresis mechanism 300 is applicable to the electrophoresis chip 2000 shown in Figures 13 to 19. The electrophoresis mechanism 300 includes M probe groups corresponding to the total number of channels 2140, where M≤N. Each probe group includes a positive probe and a negative probe. The electrophoresis operation is performed on the channels 2140 after the sample loading operation is completed, including: before the first channel 2140 completes the sample loading operation, controlling all probe groups of the electrophoresis mechanism 300 to simultaneously contact the electrode contact end 2211 of the corresponding channel, and controlling all probe groups to be energized. The entire channel is pre-energized, and a stable electric field is established in all channels before the first sample is loaded, saving the stabilization time for each channel.

[0193] For example, taking an electrophoresis chip 2000 with 16 channels 2140 for accommodating samples as an example, for example, M is 16, the electrophoresis mechanism 300 structure can have two rows of probes, with 16 probes in each row. The probe groups consisting of two corresponding probes in each row constitute positive and negative probes. The 32 probes are fixed on the electrophoresis fixing plate 310. Before the electrophoresis operation of the first channel 2140, all probes of the electrophoresis mechanism 300 are controlled to simultaneously contact the electrode contact terminal 2211 and be pre-energized to establish a stable electric field before the sample is added to the first channel 2140. After the electrophoresis operation of all channels 2140 is completed, the probe groups corresponding to all channels 2140 are controlled to be de-energized in a timely manner to reduce power consumption.

[0194] In some embodiments, there are M electrophoresis mechanisms corresponding to the total number of flow channels. Each electrophoresis mechanism includes a probe group, and each probe group includes a positive electrode probe and a negative electrode probe. The electrophoresis operation on the flow channels after the sample loading operation is completed includes: after the sample loading operation is completed in the first flow channel, controlling the probe group of the first electrophoresis mechanism to contact the electrode contact end corresponding to the first flow channel and energizing it; and after the electrophoresis operation is completed in the first flow channel, controlling the probe group of the first electrophoresis mechanism to de-energize and leave the electrode contact end corresponding to the first flow channel; after the sample loading operation is completed in the (N-1)th flow channel, controlling the (N-1)th electrophoresis mechanism to...The probe group of the structure contacts and energizes the electrode contact end corresponding to the (N-1)th flow channel. After the electrophoresis operation is completed in the (N-1)th flow channel, the probe group of the (N-1)th electrophoresis mechanism is de-energized and leaves the electrode contact end corresponding to the (N-1)th flow channel. After the sample loading operation is completed in the Nth flow channel, the probe group of the Nth electrophoresis mechanism is contacted and energized the electrode contact end corresponding to the Nth flow channel. After the electrophoresis operation is completed in the Nth flow channel, the probe group of the Nth electrophoresis mechanism is de-energized and leaves the electrode contact end corresponding to the Nth flow channel.

[0195] For example, this electrophoresis mechanism 300 is applicable to the electrophoresis chip 3000 shown in Figures 20 to 22. The electrophoresis chip 300 is provided with a plurality of flow channels 3110. Among them, there are M electrophoresis mechanisms 300 corresponding to the total number of flow channels (where M≤N, N is the total number of flow channels 3110). Each electrophoresis mechanism 300 includes a probe group, each probe group including a positive probe and a negative probe. The materials of the positive and negative probes are carefully selected, possessing good conductivity and chemical stability, and can maintain stable performance during long-term use, ensuring the accuracy and reliability of the electrophoresis operation.

[0196] The electrophoresis operation on the flow channel after the sample loading operation is completed includes: after the sample loading operation is completed in the first flow channel 3110, controlling the probe group of the first electrophoresis mechanism 300 to move and contact the corresponding contact end 3121 of the first flow channel 3110. During the movement, the speed and position of the probe group are precisely monitored and controlled to ensure accurate contact with the contact end 3121. Once contact is successful, the probe group of the first electrophoresis mechanism 300 is energized. At this time, the positive and negative probes form a complete circuit with the contact end 3121, generating a suitable electric field in the first flow channel 3110, driving the sample to be tested in the flow channel 3110 to begin electrophoretic separation. After the electrophoresis operation is completed in the first flow channel 3110, the probe group of the first electrophoresis mechanism 300 is de-energized and moves away from the contact end 3121 corresponding to the first flow channel 3110, preparing for the electrophoresis operation of the other flow channels 3110. Similarly, after the sample loading operation is completed in the (N-1)th flow channel 3110, the probe group of the (N-1)th electrophoresis mechanism 300 is energized and contacts the contact end 3121 corresponding to the (N-1)th flow channel 3110. After the electrophoresis operation is completed in the (N-1)th flow channel 3110, the probe group of the (N-1)th electrophoresis mechanism 300 is de-energized and moves away from the contact end 3121 corresponding to the (N-1)th flow channel 3110. After the sample loading operation is completed in the Nth flow channel 3110, the probe group of the Nth electrophoresis mechanism 300 is controlled to contact the corresponding contact end 3121 of the Nth flow channel 3110 and be energized. After the electrophoresis operation is completed in the Nth flow channel 3110, the probe group of the Nth electrophoresis mechanism 300 is controlled to be de-energized and...The sample leaves the contact end 3121 corresponding to the Nth channel 3110. During this process, after the sample is added to each channel 3110, only the corresponding probe group (positive probe and negative probe) contacts the contact end 3121 of the channel 3110 and is energized, realizing precise one-to-one control and improving the accuracy and flexibility of the electrophoresis process.

[0197] In some embodiments, taking pictures of each channel that has completed the electrophoresis operation to obtain the sample imaging image corresponding to each channel includes: taking pictures of the N-1th channel after the electrophoresis operation is completed in the N-1th channel to obtain the sample imaging image corresponding to the N-1th channel; taking pictures of the Nth channel after the electrophoresis operation is completed in the Nth channel to obtain the sample imaging image corresponding to the Nth channel.

[0198] For example, after the electrophoresis operation is completed in the (N-1)th channel 2140, an image is taken of the (N-1)th channel 2140 to obtain the sample imaging image corresponding to the (N-1)th channel 2140; after the electrophoresis operation is completed in the Nth channel 2140, an image is taken of the Nth channel 2140 to obtain the sample imaging image corresponding to the Nth channel 2140. Taking an image immediately after the electrophoresis operation of each channel 2140 can avoid phenomena such as band diffusion, band "blurring", and "tailing", ensuring the optimal aggregation state of the bands and maintaining the stability of the band morphology; each channel 2140 independently and separately performs the imaging operation, which can fully allocate resources and reduce the camera idle rate.

[0199] In some embodiments, the imaging mechanism has an imaging field of view covering K adjacent channels, where K ≥ 2; the method further includes: according to the electrophoresis progress prediction, controlling the imaging mechanism to move in advance to a first imaging region containing channels i to j, wherein the first imaging region simultaneously covers K adjacent channels, 1 ≤ i < M - K, j = i + K - 1; when it is detected that any channel in the first imaging region has completed the electrophoresis operation, controlling the imaging mechanism to take a picture of the channel in the first imaging region; if other channels in the first imaging region have not completed the electrophoresis operation, then keeping the position of the imaging mechanism and continuing to wait; when all channels in the first imaging region have completed the picture taking, according to the position of the next batch of channels that are about to complete the electrophoresis, moving the imaging mechanism to a second imaging region.

[0200] For example, in order to achieve precise control and efficient operation of the imaging mechanism 400, the electrophoresis progress is accurately predicted based on real-time data and historical experience of the electrophoresis operation. Specifically, the imaging mechanism 400 has an imaging field of view covering K adjacent channels 2140, where K ≥ 2. Based on the electrophoresis progress prediction, the imaging mechanism 400 can be controlled to move in advance to a first imaging region containing channels i to j 2140, wherein the first imaging region simultaneously covers K adjacent channels 2140, 1 ≤ i < M - K, j = i + K -1; Such regional division ensures the continuity of imaging and avoids wasting the imaging field of view, enabling the imaging mechanism 400 to reach the most suitable imaging position at the most suitable time.

[0201] When the imaging mechanism 400 reaches the first imaging area, it enters a waiting and monitoring state, and monitors the electrophoresis operation progress of each channel 2140 in the first imaging area in real time. When it is detected that any channel 2140 in the first imaging area has completed the electrophoresis operation, the imaging mechanism 400 is controlled to take pictures of the channel 2140 in the first imaging area; here, the method of taking pictures of all channels 2140 in the area at the same time is adopted, instead of taking pictures of each channel 2140 one by one, in order to further improve the imaging efficiency.

[0202] However, in the actual electrophoresis process, the time for each channel 2140 in the same imaging area to complete the electrophoresis operation may differ. If other channels 2140 within the first imaging region have not completed electrophoresis, the imaging mechanism 400 will not immediately leave or perform other operations, but will remain in its current position and continue to wait. This waiting mechanism ensures that the optimal imaging opportunity for any channel 2140 is not missed, guaranteeing that the sample imaging images of all channels 2140 can be acquired when the bands are in the optimal aggregation state and the morphology is stable, thus providing a reliable basis for subsequent accurate analysis.

[0203] After all channels 2140 within the first imaging region have completed imaging, the imaging mechanism 400 is moved to the second imaging region according to the position of the next batch of channels to be electrophoresed (page 20 / 26, CN 121347633 A 2140). This intelligent imaging strategy based on prediction results and the detected stage of the channel 2140 can efficiently complete the imaging work in conjunction with sample loading and electrophoresis operations.

[0204] In some embodiments, the method further includes: during electrophoresis, real-time detection of electrophoresis conditions, including electric field strength, current and / or voltage; acquisition of relevant information of samples in each channel, including at least one of sample type and sample quantity; determining whether electrophoresis parameters need to be adjusted between different channels to ensure consistency of electrophoresis effect based on the real-time detected electrophoresis conditions and the relevant information of samples in each channel; if electrophoresis parameters need to be adjusted between different channels, then the corresponding electrophoresis parameters are adjusted for different channels respectively.

[0205] For example, during electrophoresis, real-time detection of electrophoresis conditions, including electric field strength, current and / or voltage, and continuous acquisition of physical parameters of each channel through an embedded sensor network, including but not limited to: electric field strength (E = V / d, where V is voltage and d is dielectric thickness), current density (J = I / A, where I is current and A is cross-sectional area), and temperature gradient (generated by Joule heating effect);

[0206] For example, acquisition of relevant information of samples in each channel 2140, including at least one of sample type and sample quantity.At least one of the following methods is used to obtain the biological characteristics of the sample in each channel (as shown in Figure 2140) through spectral analysis or fluorescent labeling technology: sample type (DNA / RNA / protein), molecular weight distribution (which can be calculated by mobility), and concentration gradient (based on absorbance detection);

[0207] For example, based on the real-time detected electrophoresis conditions and the relevant information of the sample in each channel 2140, it is determined whether the electrophoresis parameters of different channels 2140 need to be adjusted to ensure the consistency of the electrophoresis effect; if the electrophoresis parameters of different channels 2140 need to be adjusted, the corresponding electrophoresis parameters are adjusted for each channel 2140 respectively.

[0208] In some embodiments, the method further includes: after acquiring a sample imaging image corresponding to each flow channel, performing image processing on the sample imaging image, wherein the image preprocessing includes at least one of noise removal, contrast enhancement, and image distortion correction; using an image recognition algorithm to identify the sample imaging image to obtain the brightness and position information of the strip objects in the sample imaging image; comparing the brightness and position information of the strip objects in the sample imaging image with a standard reference strip to obtain strip information in the test sample corresponding to each flow channel.

[0209] For example, after acquiring a sample imaging image corresponding to each flow channel 2140, performing image processing on the sample imaging image includes at least one of noise removal, contrast enhancement, and image distortion correction. For example, a Gaussian filtering algorithm is used to eliminate high-frequency noise in the sample imaging image while retaining strip edge features. For example, histogram equalization technology is used to enhance the contrast between the strip and the background. For example, a geometric correction algorithm is used to eliminate image distortion caused by optical system distortion.

[0210] Then, an image recognition algorithm is used to identify the sample imaging image to obtain the brightness and position information of the strip objects in the sample imaging image.

[0211] Then, the brightness and position information of the band objects in the sample imaging image are compared with the standard reference bands to obtain the band information in the sample to be tested corresponding to each channel 2140.

[0212] For example, a molecular weight standard (Ladder) is set. The sample to be tested and the molecular weight standard are separated by electrophoresis under the action of an electric field. When analyzing the obtained images, the standard reference band separated by the molecular weight standard is used as a benchmark. The information of the standard reference band separated by the molecular weight standard and the information separated by the sample to be tested (i.e., the brightness and position information of the band objects in the sample imaging image) are compared to calculate the band information in the sample to be tested corresponding to each channel 2140. The band information includes the corresponding nucleic acid fragment length, concentration and nucleic acid integrity index, etc.

[0213] For example, an electrophoresis chip with 16 available channels (such as electrophoresis chip 2000 or electrophoresis chip 3000) is set. In order to simplify the time consumed by the whole process electrophoresis, and to compare the sequential single-step whole process electrophoresis with the concurrent whole process electrophoresis of this application,difference in swimming, assuming that the running time of the three steps of pipette tip picking, sample aspiration and sample loading is all 5s, the electrophoresis time of a single flow channel is 90s, and the photographing time of a single flow channel is 2s, the remaining steps are the same, and the difference in time of the same steps corresponding to different flow channels is ignored.

[0214] The sequential single-step whole-process electrophoresis step in the conventional technology is to add samples to 16 flow channels sequentially, perform electrophoresis simultaneously and then take photographs sequentially, and the required time is: (5+5+5)×16+90+2×16=362s.

[0215] In the concurrent whole-process electrophoresis step in the embodiment of the present application, when the N-th flow channel (the flow channel 2140 on the electrophoresis chip 2000, or the flow channel 3110 on the electrophoresis chip 3000) completes the three steps of pipette tip picking, sample aspiration and sample loading, the (N-1)-th flow channel starts to take a photograph after 90s of electrophoresis, wherein 1<N≤16, N is a natural number. During the electrophoresis of this flow channel, the imaging mechanism 400 can move to the flow channel in advance to wait for photographing, and the photographing time is at the millisecond level, so the time consumed by the photographing action itself can be ignored. After the 16th flow channel completes sample loading, it is photographed after 90s of electrophoresis, at this time, 16 flow channels of the electrophoresis chip 2000 complete electrophoresis and obtain images. The required time is: (5+5+5)+15×15+90=330s.

[0216] The split timing of the concurrent whole-process electrophoresis step is shown in Figure 23. Figure 23 shows the operation timing of concurrent whole-process electrophoresis performed by 16 independent flow channels on the electrophoresis chip. Horizontally from left to right, the time increases by 15s per grid, totaling 22×15=330s; the content in the vertical grid is the operation performed at the start or end node of a time unit (15s). The operation sequence is carried out in accordance with the three operation stages in the above embodiment in sequence: the sample loading operation stage, including pipette tip picking, sample aspiration and sample loading, that is, picking / aspirating / loading sample N in the figure, which takes 15 seconds per flow channel; the electrophoresis operation stage, that is, electrophoresis N in the figure, the operation is triggered at the start node of the time period, which takes 90 seconds per flow channel; the imaging operation stage, that is, photographing N in the figure, the operation is triggered at the end node of the time period, which takes 2 seconds per flow channel.

[0217] When the process starts, the first flow channel performs picking / aspirating / loading sample 1, which takes 15s. Immediately after the sample loading operation of the first flow channel is completed, electrophoresis 1 starts, and at the same time, the second flow channel starts the sample loading operation and enters the next 15s, that is, the sample loading operation of the second flow channel is parallel with the electrophoresis operation of the first flow channel. When the operation proceeds sequentially to the end of the sample loading operation of the 6th flow channel, since the first flow channel has passed 90 seconds, the electrophoresis operation is just completed, that is, the imaging mechanism 400 immediately performs photographing 1 at the start node of the 7th 15s. Subsequently, every 15s, one flow channel will complete the sample loading operation in sequence, and after completing the sample loading operation, the electrophoresis operation is performed for the corresponding flow channel(Consuming 90 seconds, divided into five 15-second intervals), the imaging unit 400 takes a picture every 15 seconds during the seventh 15-second interval. Specifically, during the electrophoresis 1 operation in the first channel from the first 15 seconds to the sixth 15 seconds, the second to sixth sample loading operations are completed; during the seventh 15-second interval, the first picture is taken, and during this period, electrophoresis 2 to electrophoresis 5 are performed in parallel. This process continues, with the sample loading time per channel, the start / end time interval between electrophoresis operations in adjacent channels, and the time interval between two consecutive pictures all being 15 seconds. When the 16th flow channel completes the sample loading operation and enters the electrophoresis operation, the 10th flow channel has just completed the electrophoresis operation, and the imaging mechanism takes a picture 10. After the 16th flow channel starts the electrophoresis operation (electrophoresis 16), the 11th, 12th, 13th, 14th, 15th and 16th flow channels continue the electrophoresis operation, and immediately after the electrophoresis operation of each flow channel is completed, the single flow channel is photographed (picture 11 to picture 15) until the 16th flow channel completes the electrophoresis operation and takes a picture 16, and the whole process ends.

[0218] The total time for concurrent full-process electrophoresis is 330s, which saves 32s compared to the total time of 362s for sequential single-step full-process electrophoresis. It also ensures that the obtained image is the most realistic band state at the moment of completion of electrophoresis, greatly reducing the lag in the acquired image caused by the delay in taking pictures. In the concurrent full-process electrophoresis steps, the time interval between the addition of the sample to be tested in each channel and the start of electrophoresis is almost completely consistent, avoiding the need to wait until the last channel is added after the first channel is added before electrophoresis can start. This reduces the risk of sample diffusion in the buffer chamber, further ensuring the consistency of the sample in each channel and improving the reliability of electrophoresis image data analysis.

[0219] The conclusions of electrophoresis data are derived from the brightness and position information of the bands in the image taken after electrophoresis. The band information of the target sample is calculated by comparing it with the bands of the standard reference. Therefore, the image taken directly affects the accuracy of the result calculation. In the sequential single-step full-process electrophoresis procedure, by sequentially loading all channels, performing electrophoresis simultaneously, and then taking photographs sequentially, inconsistencies arise in the time the sample remains in the buffer chamber after loading in each channel (as described on pages 22 / 26 of the instruction manual, CN 121347633 A), and also in the time it takes to photograph the bands after electrophoresis. Both of these time differences affect the inconsistency between the initial and final states of the sample test. The longer the sample remains in the buffer chamber after loading, the more severe the dispersion of the sample in the buffer, and the more significantly it affects the accuracy of the band measurement. In the sequential single-step full-process electrophoresis, after loading the sample in the first channel, a waiting time of 15 × 15 = 225 seconds is required before starting electrophoresis, while after loading the sample in the 16th channel, no waiting time is required to start electrophoresis. After the sample in the 16th channel is electrophoresed, a waiting time of 15 × 2 = 25 seconds is required.It takes 30 seconds to take a picture, but the first flow channel can be photographed immediately after electrophoresis. Therefore, the same sample is loaded into the first and 16th flow channels, and the time difference between the initial and final states accumulates to 255 seconds. For short fragments and low concentrations of sample, the measurement results are easily distorted. In contrast, the concurrent full-process electrophoresis steps provided in this application can completely avoid these two time differences, so that the sample is added to the buffer chamber and electrophoresis starts immediately, or the sample stays in the buffer chamber for the same amount of time, and the picture is taken immediately after the electrophoresis of each flow channel is completed, so as to obtain the most timely and realistic sample band image.

[0220] All the above technical solutions can be combined in any way to form optional embodiments of this application, and will not be described in detail here.

[0221] The sample imaging method provided in this application embodiment is applied to an electrophoresis chip. The electrophoresis chip has multiple channels for accommodating the sample to be tested. In this method, a sample loading operation is performed sequentially on each channel, and an electrophoresis operation is performed on the channel after the sample loading operation is completed, so that the sample to be tested in the channel is separated by electrophoresis. Specifically, after the sample loading operation is completed on the (N-1)th channel, a sample loading operation is performed on the Nth channel, and an electrophoresis operation is performed on the (N-1)th channel. After the sample loading operation is completed on the Nth channel, an electrophoresis operation is performed on the Nth channel. 1 < N ≤ the total number of channels, where N is a natural number. Each channel that has completed the electrophoresis operation is photographed to obtain a sample imaging image corresponding to each channel. This application embodiment effectively shortens the total time required for sample imaging by performing the sample loading operation and electrophoresis operation in parallel, improves the efficiency and continuity of the sample imaging process, and improves the detection quality.

[0222] To facilitate better implementation of the sample imaging method of this application embodiment, this application embodiment also provides a sample imaging device 20, as shown in Figure 24. The sample imaging device 20 can provide a graphical user interface through a terminal device. The graphical user interface includes at least a partial virtual scene and at least one virtual character. The sample imaging device 20 may include:

[0223] a processing unit 21, used to sequentially perform sample loading operations on each of the flow channels and perform electrophoresis operations on the flow channels after the sample loading operation is completed, so that the test sample in the flow channel is separated by electrophoresis. After the sample loading operation is completed in the (N-1)th flow channel, the sample loading operation is continued in the Nth flow channel, and the electrophoresis operation is performed in the (N-1)th flow channel. After the sample loading operation is completed in the Nth flow channel, the electrophoresis operation is performed in the Nth flow channel, 1 < N ≤ the total number of flow channels, and N is a natural number;

[0224] an acquisition unit 22, used to take pictures of each flow channel that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each flow channel.

[0225] In some embodiments, each flow channel in the electrophoresis chip is misaligned with the electrode contact end corresponding to each flow channel.The configuration is as follows: when the probe of the electrophoresis mechanism for performing electrophoresis contacts the electrode contact end corresponding to each channel in the electrophoresis chip, the movement path of the sample loading mechanism for performing sample loading does not overlap with the movement path of the probe in spatial projection.

[0226] In some embodiments, the sample loading mechanism further includes a puncture needle; when the probe contacts the electrode contact end corresponding to each channel in the electrophoresis chip, the movement path of the puncture needle does not overlap with the movement path of the probe in spatial projection. In some embodiments, for non-edge channels, the corresponding electrode contact end is located in the interval region between adjacent channels; for edge channels, the corresponding electrode contact end is located on the side of the corresponding channel away from the adjacent channel; or the electrode contact end corresponding to one edge channel is located on the side of the corresponding channel away from the adjacent channel, and the electrode contact end corresponding to another edge channel is located in the interval region between the other edge channel and the adjacent channel, so that each channel in the electrophoresis chip is misaligned with the electrode contact end corresponding to each channel.

[0228] In some embodiments, the electrophoresis mechanism includes M sets of probes corresponding to the total number of channels. Each set of probes includes a positive probe and a negative probe. The processing unit 21 is used to perform electrophoresis on the channels after the sample loading operation has been completed, including: before the sample loading operation is completed in the first channel, controlling all probes of the electrophoresis mechanism to simultaneously contact the electrode contact end of the corresponding channel; after the sample loading operation is completed in the (N-1)th channel, controlling the probes corresponding to the (N-1)th channel to be energized to perform electrophoresis on the (N-1)th channel; after the sample loading operation is completed in the Nth channel, controlling the probes corresponding to the Nth channel to be energized to perform electrophoresis on the Nth channel.

[0229] In some embodiments, the processing unit 21 is further configured to: after the Nth channel completes the electrophoresis operation, control the probe group corresponding to the Nth channel to be de-energized; for the remaining channels that have not completed the sample loading operation, maintain the physical contact between the corresponding probe group and the electrode contact end, but keep them in a de-energized state until the channel completes the sample loading operation and is ready to perform the electrophoresis operation, and then control the corresponding probe group to be energized.

[0230] In some embodiments, the electrophoresis mechanism includes M groups of probe groups corresponding to the total number of channels, each group of probe groups includes a positive electrode probe and a negative electrode probe, and the processing unit 21 is configured to perform electrophoresis operation on the channels that have completed the sample loading operation, including: before the first channel completes the sample loading operation, controlling all probe groups of the electrophoresis mechanism to simultaneously contact the electrode contact end of the corresponding channel, and controlling all probe groups to be energized.

[0231] In some embodiments, there are M electrophoresis mechanisms corresponding to the total number of flow channels. Each electrophoresis mechanism includes a set of probes, and each set of probes includes a positive electrode probe and a negative electrode probe. The processing unit 21 is used to process the electrophoresis mechanism after the sample loading operation is completed.Electrophoresis is performed in the flow channel, including:

[0232] After the sample loading operation is completed in the first flow channel, the probe group of the first electrophoresis mechanism is controlled to contact and be energized with the electrode contact end corresponding to the first flow channel. After the electrophoresis operation is completed in the first flow channel, the probe group of the first electrophoresis mechanism is controlled to be de-energized and move away from the electrode contact end corresponding to the first flow channel. After the sample loading operation is completed in the (N-1)th flow channel, the probe group of the (N-1)th electrophoresis mechanism is controlled to contact and be energized with the electrode contact end corresponding to the (N-1)th flow channel. After the electrophoresis operation is completed in the (N-1)th flow channel, the probe group of the (N-1)th electrophoresis mechanism is controlled to be de-energized and move away from the electrode contact end corresponding to the (N-1)th flow channel. After the sample loading operation is completed in the (N)th flow channel, the probe group of the (N)th electrophoresis mechanism is controlled to contact and be energized with the electrode contact end corresponding to the (N)th flow channel. After the electrophoresis operation is completed in the (N)th flow channel, the probe group of the (N)th electrophoresis mechanism is controlled to be de-energized and move away from the electrode contact end corresponding to the (N)th flow channel.

[0233] In some embodiments, the acquisition unit 22 is configured to: take a picture of the N-1th flow channel after the electrophoresis operation is completed in the N-1th flow channel to obtain a sample imaging image corresponding to the N-1th flow channel; and take a picture of the Nth flow channel after the electrophoresis operation is completed in the Nth flow channel to obtain a sample imaging image corresponding to the Nth flow channel.

[0234] In some embodiments, the imaging mechanism has an imaging field of view covering K adjacent channels, where K ≥ 2; the acquisition unit 22 is further configured to: control the imaging mechanism to move in advance to a first imaging region containing channels i to j according to the electrophoresis progress prediction, wherein the first imaging region simultaneously covers K adjacent channels, 1 ≤ i < M - K, j = i + K - 1; when it is detected that any channel in the first imaging region has completed the electrophoresis operation, control the imaging mechanism to take pictures of the channels in the first imaging region;

[0235] if other channels in the first imaging region have not completed the electrophoresis operation, the position of the imaging mechanism is maintained and the process continues to wait; when all channels in the first imaging region have completed the picture taking, the imaging mechanism is moved to a second imaging region according to the position of the next batch of channels that are about to complete the electrophoresis.

[0236] In some embodiments, the processing unit 21 is used to sequentially perform a sample loading operation on each of the plurality of flow channels, including: sequentially performing the operations of picking up the pipette tip, aspirating the sample, and loading the sample on each of the plurality of flow channels.

[0237] In some embodiments, the sample loading mechanism for performing the sample loading operation includes a puncture needle and a sample loading needle. The processing unit 21 is used to sequentially perform the operations of picking up the pipette tip, aspirating the sample, and loading the sample on each of the plurality of flow channels, including: controlling the puncture needle to perform a puncture operation on the target flow channel; after the puncture operation is completed, controlling the sample loading needle to sequentially advance into the target flow channel.The process involves taking the pipette tip, aspirating the sample, and loading the sample to be tested into the punctured target flow channel.

[0238] In some embodiments, the sample loading mechanism for performing the sample loading operation includes a puncture needle and a sample loading needle. The processing unit 21 is used to sequentially perform the pipette tip, aspirate the sample, and load the sample into each of the plurality of flow channels, including: controlling the sample loading needle to sequentially perform the pipette tip and aspirate the sample; controlling the puncture needle to perform a puncture operation into the target flow channel; and controlling the sample loading needle to load the sample to be tested into the target flow channel after the puncture operation is completed.

[0239] In some embodiments, before sequentially performing the sample loading operation into each of the plurality of flow channels, the processing unit 21 is further used to: pre-treat the electrophoresis chip, the pre-treatment including at least one of cleaning, drying, and dust removal.

[0240] In some embodiments, before sequentially performing sample loading operations on each of the plurality of channels, the processing unit 21 is further configured to: perform calibration processing on the sample loading mechanism used for performing the sample loading operation, the calibration processing including at least one of vertical positioning calibration, horizontal position calibration, and sample loading volume calibration.

[0241] In some embodiments, the processing unit 21 is further configured to: during the electrophoresis process, detect electrophoresis conditions in real time, the electrophoresis conditions including electric field strength, current and / or voltage; acquire relevant information of the sample in each channel, the relevant information including at least one of sample type and sample volume; determine whether it is necessary to adjust the electrophoresis parameters between different channels to ensure the consistency of the electrophoresis effect based on the real-time detected electrophoresis conditions and the relevant information of the sample in each channel; if it is necessary to adjust the electrophoresis parameters between different channels, adjust the corresponding electrophoresis parameters for each channel respectively.

[0242] In some embodiments, the acquisition unit 22 is further configured to: after acquiring a sample imaging image corresponding to each flow channel, perform image processing on the sample imaging image, wherein the image preprocessing includes at least one of noise removal, contrast enhancement, and image distortion correction; identify the sample imaging image using an image recognition algorithm to obtain the brightness and position information of the strip objects in the sample imaging image; and compare the brightness and position information of the strip objects in the sample imaging image with a standard reference strip to obtain the strip information in the sample to be tested corresponding to each flow channel.

[0243] Each unit in the above-described sample imaging device can be implemented entirely or partially by software, hardware, or a combination thereof. Each unit can be embedded in or independent of the processor in a computer device in hardware form, or stored in the memory of a computer device in software form, so that the processor can call and execute the operations corresponding to each unit.

[0244] The sample imaging device 20 can be integrated into a terminal or server with storage and a processor installed and having computing power, or the sample imaging device 20 can be the terminal or server.

[0245] All the above technical solutions can be combined in any way to form optional embodiments of this application, and will not be described in detail here.

[0246] It should be understood that the sample imaging device 20 embodiment and the sample imaging method embodiment can correspond to each other, and similar descriptions can be referred to the method embodiment. To avoid repetition, it will not be described in detail here. Specifically, the sample imaging device 20 can execute the above sample imaging method embodiment, and the foregoing and other operations and / or functions of each unit in the sample imaging device 20 respectively implement the corresponding process of the above method embodiment. For the sake of brevity, it will not be described in detail here. Specification 25 / 26 pages 28 CN 121347633 A

[0247] Optionally, this application also provides a computer device, including a memory and a processor, wherein the memory stores a computer program, and the processor executes the computer program to implement the steps in the above method embodiments.

[0248] FIG25 is a schematic diagram of the structure of the computer device provided in the embodiment of this application. The computer device can be a terminal or a server. As shown in FIG25, the computer device 30 may include: a communication interface 31, a memory 32, a processor 33 and a communication bus 34. The communication interface 31, memory 32, and processor 33 communicate with each other via the communication bus 34. The communication interface 31 is used for data communication between the computer device 30 and external devices. The memory 32 can be used to store software programs and modules. The processor 33 is the control center of the computer device 30, connecting all parts of the computer device 30 through various interfaces and lines. By running or loading software programs and / or modules stored in the memory 32, and by calling data stored in the memory 32, it executes various functions of the computer device 30 and processes data, thereby performing overall processing of the computer device 30.

[0249] In this embodiment of the application, the processor 33 in the computer device 30 will load the instructions corresponding to the process of one or more computer programs into the memory 32 according to the following steps, and the processor 33 will run the computer program stored in the memory 32 to realize various functions:

[0250] Each channel in the flow channel is sequentially sampled, and the channel that has completed the sampled operation is electrophoretically separated so that the sample to be tested in the channel is separated by electrophoresis. After the sampled operation is completed in the N-1th channel, the Nth channel is sampled and electrophoretic operation is performed in the N-1th channel. After the sampled operation is completed in the Nth channel, the Nth channel is electrophoretically separated. 1 < N ≤ the total number of channels, and N is a natural number. Each channel that has completed the electrophoretic operation is photographed to obtain the sample imaging image corresponding to each channel.

[0251] Those skilled in the art will understand that all or part of the steps in the structural design method of the above embodiments areThis can be accomplished through instructions or by controlling related hardware through instructions. The instructions can be stored in a computer-readable storage medium and loaded and executed by a processor.

[0252] To this end, embodiments of this application provide a computer-readable storage medium storing multiple computer programs. These computer programs can be loaded by a processor to execute the steps in any of the data communication methods provided in embodiments of this application. Specific implementations of the above operations can be found in the preceding embodiments and will not be repeated here.

[0253] The storage medium may include: read-only memory (ROM), random access memory (RAM), disk, or optical disk, etc.

[0254] Since the computer programs stored in the storage medium can execute the steps in any of the sample imaging methods provided in embodiments of this application, the beneficial effects achievable by any of the sample imaging methods provided in embodiments of this application can be realized. See the preceding embodiments for details, and will not be repeated here.

[0255] Embodiments of this application also provide a computer program product, which includes computer instructions stored in a computer-readable storage medium. The processor of the computer device reads the computer instructions from a computer-readable storage medium, and executes the computer instructions, causing the computer device to perform the corresponding process in any of the sample imaging methods in the embodiments of this application. For simplicity, it will not be described in detail here.

[0256] The embodiments of this application also provide a computer program, which includes computer instructions stored in a computer-readable storage medium. The processor of the computer device reads the computer instructions from the computer-readable storage medium, and executes the computer instructions, causing the computer device to perform the corresponding process in any of the sample imaging methods in the embodiments of this application. For simplicity, it will not be described in detail here.

[0257] The above descriptions are only specific embodiments of this application, but the protection scope of this application is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this application should be included within the protection scope of this application. Therefore, the protection scope of this application should be determined by the protection scope of the claims. Instruction manual, page 26 / 26, page 29, CN 121347633 A, Figure 1, Figure 2, Appendix 1 / 12, page 30, CN 121347633 A, Figure 3, Figure 4, Appendix 2 / 12, page 31, CN 121347633 A, Figure 5, Figure 6, Appendix 3 / 12, page 32, CN 121347633 A, Figure 7, Figure 8, Appendix 4 / 12, page 33, CN 121347633 AFigure 9 Figure 10 Appendix to the Instruction Manual 5 / 12 Page 34 CN 121347633 A Figure 11 Figure 12 Appendix to the Instruction Manual 6 / 12 Page 35 CN 121347633 A Figure 13 Figure 14 Appendix to the Instruction Manual 7 / 12 Page 36 CN 121347633 A Figure 15 Figure 16 Appendix to the Instruction Manual 8 / 12 Page 37 CN 121347633 A Figure 17 Figure 18 Figure 19 Appendix to the Instruction Manual 9 / 12 Page 38 CN 121347633 A Figure 20 Figure 21 Appendix to the Instruction Manual 10 / 12 Page 39 CN 121347633 A Figure 22 Figure 23 Figure 24 Appendix to the Instruction Manual 11 / 12 Page 40 CN 121347633 A Figure 25 Appendix to the Instruction Manual 12 / 12 Page 41 CN 121347633 A Abstract This application discloses a sample imaging method, apparatus, storage medium, device, and program product. The method is applied to an electrophoresis chip, which has multiple channels for accommodating samples to be tested.on the Nth channel, and electrophoresis operation is performed on the (N-1)th channel; after the Nth channel completes the sample loading operation, electrophoresis operation is performed on the Nth channel, where 1 < N ≤ the total number of channels, and N is a natural number; photographing each channel that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel. This application effectively shortens the total time required for sample imaging by performing sample loading operations and electrophoresis operations in parallel, thereby improving the efficiency and continuity of the sample imaging process and enhancing the detection quality.

Claims

1. A method of imaging a sample, characterized by, The application is applied to an electrophoresis chip having a plurality of flow channels for containing samples to be tested, and the method comprises the following steps: sequentially performing sample adding operation on each of the flow channels, and performing electrophoresis operation on the flow channels after sample adding operation is completed, so that the samples to be tested in the flow channels complete electrophoresis separation, wherein, after the N-1th flow channel completes sample adding operation, sample adding operation is performed on the Nth flow channel, and electrophoresis operation is performed on the N-1th flow channel, after the Nth flow channel completes sample adding operation, electrophoresis operation is performed on the Nth flow channel, 1 photographing each flow channel after electrophoresis operation is completed to obtain sample imaging images corresponding to each flow channel.

2. The sample imaging method of claim 1, wherein, Each flow channel in the electrophoresis chip is arranged in a staggered manner with the electrode contact end corresponding to each flow channel. When a probe of an electrophoresis mechanism for performing electrophoresis operation contacts the electrode contact end corresponding to each flow channel in the electrophoresis chip, the moving path of a sample adding needle of a sample adding mechanism for performing sample adding operation does not overlap with the moving path of the probe in space projection.

3. The sample imaging method of claim 2, wherein, For non-edge flow channels, the corresponding electrode contact end is located in the interval region between adjacent flow channels, for edge flow channels, the corresponding electrode contact end is located on the side of the corresponding flow channel away from the adjacent flow channel, or the electrode contact end of one edge flow channel is located on the side of the corresponding flow channel away from the adjacent flow channel, and the electrode contact end of the other edge flow channel is located in the interval region between the other edge flow channel and the adjacent flow channel, so that each flow channel in the electrophoresis chip is arranged in a staggered manner with the electrode contact end corresponding to each flow channel.

4. The method of claim 2, wherein, The electrophoresis mechanism comprises M groups of probes corresponding to the total number of flow channels, each group of probes comprises a positive probe and a negative probe, and the electrophoresis operation on the flow channels after sample adding operation is completed comprises the following steps: Before the 1st flow channel completes sample adding operation, control all groups of probes of the electrophoresis mechanism to simultaneously contact the electrode contact end of the corresponding flow channel; After the N-1th flow channel completes sample adding operation, control the group of probes corresponding to the N-1th flow channel to be electrified to perform electrophoresis operation on the N-1th flow channel; After the Nth flow channel completes sample adding operation, control the group of probes corresponding to the Nth flow channel to be electrified to perform electrophoresis operation on the Nth flow channel.

5. The method of imaging a sample of claim 1, wherein, The photographing of each flow channel after electrophoresis operation is completed to obtain sample imaging images corresponding to each flow channel comprises the following steps: After the N-1th flow channel completes electrophoresis operation, photograph the N-1th flow channel to obtain sample imaging images corresponding to the N-1th flow channel; After the Nth flow channel completes electrophoresis operation, photograph the Nth flow channel to obtain sample imaging images corresponding to the Nth flow channel.

6. The method of imaging a sample of claim 4, wherein, The imaging mechanism has an imaging field of view covering K adjacent flow channels, K≥2; and the method further comprises the following steps: According to electrophoresis progress prediction, control the imaging mechanism to move to a first imaging area containing the i-th to the j-th flow channels in advance, wherein the first imaging area simultaneously covers K adjacent flow channels, 1 When it is monitored that any flow channel in the first imaging area completes electrophoresis operation, the imaging mechanism is controlled to take a photo of the flow channel in the first imaging area; If other flow channels in the first imaging area do not complete electrophoresis operation, the imaging mechanism position is kept unchanged to continue waiting; When all flow channels in the first imaging area complete photo taking, the imaging mechanism is moved to a second imaging area according to the position of the next batch of flow channels which are about to complete electrophoresis.

7. A sample imaging apparatus, characterized by, The device is applied to an electrophoresis chip having a plurality of flow channels for containing samples to be tested, and comprises: a processing unit configured to sequentially perform sample adding operation on each of the flow channels and perform electrophoresis operation on the flow channels which complete sample adding operation, so that the samples to be tested in the flow channels complete electrophoresis separation, wherein after the (N-1)th flow channel completes sample adding operation, sample adding operation is continued on the Nth flow channel, and electrophoresis operation is performed on the (N-1)th flow channel, after the Nth flow channel completes sample adding operation, electrophoresis operation is performed on the Nth flow channel, 1 a obtaining unit configured to take a photo of each flow channel which completes electrophoresis operation to obtain a sample imaging image corresponding to each flow channel.

8. A computer-readable storage medium, characterized in that, The computer readable storage medium stores a computer program which is adapted to be loaded by the processor to execute the sample imaging method according to any one of claims 1-6.

9. A computer device, comprising: The computer device comprises a processor and a memory, and the memory stores a computer program, and the processor is configured to execute the sample imaging method according to any one of claims 1-6 by calling the computer program stored in the memory.

10. A computer program product comprising computer instructions, characterized in that, The computer instructions are executed by the processor to implement the sample imaging method according to any one of claims 1-6.

Images