Combination of ruxolitinib with INCB057643 for use in the treatment of myeloproliferative neoplasms

Abstract

The present application relates to treatment of myeloproliferative neoplasms using the JAK1 / JAK2 inhibitor, ruxolitinib, in combination with a BET protein inhibitor, 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, wherein the combination is unexpectedly synergistic.

Description

(19) *EP004691563A2* (11) EP 4 691 563 A2 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: 11.02.2026 Bulletin 2026 / 07 (21) Application number: 25209713.4 (22) Date of filing: 14.09.2020 (51) International Patent Classification (IPC): A61P 35 / 00 (2006.01) (52) Cooperative Patent Classification (CPC): (C-Sets available) A61K 31 / 538; A61K 31 / 519; A61P 35 / 00 (Cont.) (84) Designated Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated Extension States: BA ME Designated Validation States: KH MA MD TN (30) Priority: 03.06.2020 US 202063034214 P (62) Document number(s) of the earlier application(s) in accordance with Art. 76 EPC: 20781202.5 / 4 161 528 (71) Applicant: Incyte Corporation Wilmington, DE 19803 (US) (72) Inventors: • STUBBS, Matthew Wilmington, Delaware, 19803 (US) • LIU, Phillip C. Wilmington, Delaware, 19803 (US) (74) Representative: Carpmaels & Ransford LLP One Southampton Row London WC1B 5HA (GB) Remarks: •This application was filed on 20‑10‑2025 as a divisional application to the application mentioned under INID code 62. •Claims filed after the date of filing of the application (Rule 68(4) EPC). (54) COMBINATION OF RUXOLITINIB WITH INCB057643 FOR USE IN THE TREATMENT OF MYELOPROLIFERATIVE NEOPLASMS (57) The present application relates to treatment of myeloproliferative neoplasms using the JAK1 / JAK2 in- hibitor, ruxolitinib, in combination with a BET protein inhibitor, 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihy- dro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfo- nyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, wherein the combi- nation is unexpectedly synergistic. EP 4 69 1 56 3 A 2 Processed by Luminess, 75001 PARIS (FR) (Cont. next page) (52) Cooperative Patent Classification (CPC): (Cont.) C-Sets A61K 31 / 519, A61K 2300 / 00; A61K 31 / 538, A61K 2300 / 00 2 EP 4 691 563 A2 Description PRIORITY CLAIM

[0001] This application claims priority to U.S. Provisional Patent Application No. 63 / 034,214, filed on June 3, 2020, the entirety of which is incorporated by reference herein. TECHNICAL FIELD

[0002] The present application relates to treatment of myeloproliferative neoplasms using the JAK1 / JAK2 inhibitor, ruxolitinib, in combination with a BET protein inhibitor, 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c] pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, wherein the combination is unexpectedly synergistic. BACKGROUND

[0003] Compound 1 is a small molecule inhibitor of the acyl binding bromodomains found within Bromodomain and Extraterminal Domain (BET) proteins. The four family members (BRD2, 3, 4, T) facilitate transcription by binding acetylated histones and recruiting transcription initiation and elongation complexes, thereby acting as a link between chromatinand transcriptional activation (seeShi, J., andVakoc,C.R., "TheMechanismsBehind theTherapeuticActivity of BET Bromodomain Inhibition" Molecular Cell 2014; 54, 728‑736, which is incorporated herein by reference in its entirety).

[0004] The BRD4 (and occasionally BRD3) gene is a component of a chromosomal translocation which yields an aggressive subtype of squamous carcinomas termed "NUT midline" carcinoma. These midline carcinomas are definedby t(15;19) translocationswhich give rise to theBRD4-NUToncogene. The resultant fusionprotein consists ofBRD4at theN- terminus and NUT (nuclear protein in testis) at the C-terminus. Removal of this protein from chromatin, either by RNA silencingor smallmolecule inhibitors, results indifferentiationandgrowtharrest in thesecarcinomas (seeFilippakopoulos, P., et al., Selective Inhibition of BET Bromodomains. Nature 2010; 468, 1067‑1073, which is incorporated herein by reference in its entirety).

[0005] TheBETproteinsare thought toexert proliferativeeffectsby facilitating transcriptionofoncogenessuchasc-myc among others. BRD4 inhibiting compounds have been shown to prevent BRD4 from associating with chromatin, including on the c-myc promoter, in multiple myeloma models. This dissociation leads to decreased levels of c-myc expression and decreased cell viability (see Delmore, J.E., et al., "BET Bromodomain Inhibition as a Therapeutic Strategy to Target c- MYC" Cell 2011; 146, 904‑917, which is incorporated herein by reference in its entirety). BRD4 has recently been discovered tobind to "super-enhancers"whicharevast transcriptional regulatory elementswithin genesnecessary for cell fate and survival. An example is found in multiple myeloma, where the c-myc gene was found to have a super-enhancer region thatwasboundbyBRD4,giving rise tohigh levelsof c-myc transcription (seeLoven, J., et al., "Selective Inhibitionof Tumor Oncogenesby Disruption of Super-Enhancers" Cell 2013; 153, 320‑334, which is incorporated herein by reference in its entirety).

[0006] The MPLW515L mutation is found in a small percentage of patients with Philadelphia-chromosome negative myeloproliferative neoplasms (MPN). Thismutation causes constitutive activation of JAK2, leading to enhanced signaling through its downstreamtargetsandsubsequent increased levelsof cell proliferation.As there ismuchoverlapbetween the JAK / STATsignaling pathwayand the inflammatory pathways regulated transcriptionally by theBETproteins, combination of BET and JAK inhibitors in MPN may form the bases for a new therapeutic treatment protocol.

[0007] There is aneed for new therapies to improvepatient outcome.This application is directed to this needandothers. SUMMARY

[0008] The present application provides, inter alia, methods of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c] pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and rux- olitinib, or a pharmaceutically acceptable salt thereof.

[0009] The present application also provides methods of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyri- din‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and a dose of from about 5 mg / day to about 60 mg / day on a free base basis of ruxolitinib, or a pharmaceutically acceptable salt thereof.

[0010] The present application further provides the use of the compounds at the doses recited herein for use in the manufacture of medicaments for use in treating a myeloproliferative neoplasm.

[0011] The present application also provides the compounds at the doses recited herein for use in treating a myeloproliferative neoplasm. 3 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55

[0012] The details of one or more embodiments are set forth in the description below. Other features, objects, and advantages will be apparent from the description and from the claims. DESCRIPTION OF THE FIGURES

[0013] FIG. 1A is a graph depicting efficacy of Compound 1 in the HEL xenograft model, tumor volume (mm3) vs. days post inoculation. FIG. 1B is a graph depicting tolerability of Compound 1 in the HEL xenograft model, tumor corrected body weight change (%) vs. days post inoculation. FIG. 2A is a graph depicting efficacy of the combination of Compound 1 and ruxolitinib in the SET‑2 model, tumor volume (mm3) vs. days post inoculation. FIG. 2B is a graph depicting tolerability of the combination of Compound 1 and ruxolitinib in the SET‑2 model, tumor corrected body weight change (%) vs. days post inoculation. FIG. 3A is a graph depicting efficacy of the combination of Compound 1 and ruxolitinib in the SET‑2 model, tumor volume (mm3) vs. days post inoculation. FIG. 3B is a graph depicting tolerability of the combination of Compound 1 and ruxolitinib in the SET‑2 model, tumor corrected body weight change (%) vs. days post inoculation. FIG. 4A is a graph depicting spleen weight and white blood cell counts as measures of efficacy of Compound 1 and ruxolitinib in the MPLW515L mouse model of MPN. FIG. 4B is a graph depicting spleen weight and white blood cell counts as measures of efficacy of Compound 1 and ruxolitinib in the MPLW515L mouse model of MPN. FIG. 5 is a table (Table 3) depicting a summary of Compound 1 pharmacokinetic parameters for Compound 1 as monotherapy (part 1 and 2) at steady-state (cycle 1 day 8). FIG. 6 is a graph depicting inhibition of cMyc protein expression in the pharmacodynamics spiked cell assay versus plasma levels of Compound 1. FIG. 7 is a graph depicting Compound 1 steady-state plasma concentrations (mean ± se) in participants following once daily dosing of Compound 1 as monotherapy. FIG. 8 is a graph depicting probability of response (hyperglycemia) versus Compound 1 steady-state AUC following once daily dosing of Compound 1 as monotherapy. DETAILED DESCRIPTION

[0014] For the terms "e.g." and "such as," and grammatical equivalents thereof, the phrase "and without limitation" is understood to follow unless explicitly stated otherwise.

[0015] As used herein, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise.

[0016] As used herein, the term "about" means "approximately" (e.g., plus or minus approximately 10% of the indicated value).

[0017] As used herein, the phrase "solid form" refers to a compound provided herein in either an amorphous state or a crystalline state ("crystalline form" or "crystalline solid" or "crystalline solid form"), whereby a compound provided herein in a crystalline state may optionally include solvent or water within the crystalline lattice, for example, to form a solvated or hydrated crystalline form. The term "hydrated," as used herein, is meant to refer to a crystalline form that includes water molecules in the crystalline lattice. Example "hydrated" crystalline forms include hemihydrates, monohydrates, dihy- drates, and the like.Other hydrated formssuchaschannel hydratesand the likearealso includedwithin themeaningof the term. 4 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55

[0018] The present invention relates to the use of a JAK1 / JAK2 inhibitor, ruxolitinib, in combination with a BET protein inhibitor for treatment of a myeloproliferative neoplasm. Ruxolitinib, (3R)‑3-cyclopentyl‑3‑[4‑(7H-pyrrolo[2,3-d]pyrimi- din‑4-yl)‑1H-pyrazol‑1-yl]propanenitrile, is an inhibitor of JAK1andJAK2.The IC50 of ruxolitinibwasmeasuredbyAssayA infra at 1 mM ATP and found to be less than 10 nM at JAK1 and JAK2. Ruxolitinib can be made by the procedure described in US 7,598,257 (Example 67), filed December 12, 2006, which is incorporated herein by reference in its entirety. Ruxolitinib phosphate can be prepared as described in US 2008 / 0312259, which is incorporated herein by reference in its entirety.

[0019] It has been demonstrated that BET inhibition inhibits inflammatory signaling in myeloproliferative neoplasms (see Kleppe M, Koche R, Zou L, et al., "Dual targeting of oncogenic activation and inflammatory signaling increases therapeutic efficacy in myeloproliferative neoplasms". Cancer Cell 2018; 33:29‑43.e7, which is incorporated herein by reference in its entirety). In the referenced study, the BET inhibitor in combination with JAK inhibition reduced pathologic cytokine production and overall disease burden. Specifically, in an in vivo model of myeloproliferative neoplasm, combination treatment with ruxolitinib and JQ1, a JAK1 / 2 and a BET inhibitor respectively, resulted in decreased inflammation, reduced disease burden. The treatment also eliminated fibrosis in myelofibrosis mice, a response not previously observed with single-agent JAK inhibitor therapy. Mechanistically it is proposed that the synergistic effects of JAK and BET inhibitors are mediated by blocking 2 master regulators of pathologic inflammatory signaling, JAK / STATand NF-kB, respectively. Taken together, these data warrant evaluation of BET inhibitors in malignancies characterized by underlying inflammation, such as myeloproliferative neoplasms, including primary myelofibrosis.

[0020] The present application provides a method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one (Compound 1 below) is an inhibitor of BET proteins such as BRD2, BRD3, BRD4, and BRD-t. Compound 1 can be prepared as described in US Patent 9,540,368 or US Patent 10,189,832, each of which is incorporated herein by reference in its entirety.

[0021] The present application provides a method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof. Administering a combination of Compound 1 and ruxolitinib can provide an enhanced efficacy. As demonstrated by the examples provided herein, combination of Compound 1 and ruxolitinib showed enhanced efficacy in in vivo models and significantly (p < 0.05) more efficacious than either ruxolitinib alone or Compound 1 alone. That is, the combination of ruxolitinib and Compound 1 provides efficacy better than the sum of the parts.

[0022] The present application provides a method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof.

[0023] The present application further provides a method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyri- din‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and a thera- peutically effective amount of ruxolitinib, or a pharmaceutically acceptable salt thereof.

[0024] The present application further provides a method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient a dose from about 2 mg / day to about 20 mg / day on a free base basis of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxa- zin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and a therapeutically effective amount of ruxolitinib, or a pharmaceutically acceptable salt thereof.

[0025] In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- 5 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2 mg / day to about 18 mg / day on a free base basis.

[0026] In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2 mg / day to about 12 mg / day on a free base basis.

[0027] In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 4 mg / day to about 8 mg / day on a free base basis.

[0028] For example, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2, about 4, about 6, about 10, about 12, about 14, about 16, about 18, or about 20 mg / day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfo- nyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2, about 4, about 6, about 8, about 10, about 12, about 14, about 16, about 18, orabout 20mg / dayona freebasebasis. In someembodiments, thedose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxa- zin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2, about 4, about 6, about 8, about 10, or about 12mg per day ona free basebasis. In someembodiments, the doseof 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or apharmaceutically acceptable salt there- of, is about 4, about 6, or about 8 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6- methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharma- ceutically acceptable salt thereof, is about 8, about 10, about 12, about 14, or about 16 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methyl- sulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 10, about 12, about 14, about 16 mg, or about 18 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6- methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharma- ceutically acceptable salt thereof, is about 2 mg per day on a free base basis. In some embodiments, the dose of 2,2,4- trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxa- zin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 4 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfo- nyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 6 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 8 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo [2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 10 mg per day on a free base basis. In some embodiments, the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7- dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically accepta- ble salt thereof, is about 12 mg per day on a free base basis.

[0029] In some embodiments, 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methyl- sulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceu- tically acceptable salt thereof are administered once daily (QD). In some embodiments, 2,2,4-trimethyl‑8‑(6-methyl‑7- oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof are administered twice daily (BID).

[0030] In some embodiments, the dose of ruxolitinib, or a pharmaceutically acceptable salt thereof, is about 5 mg / day to about 60mg / dayona freebasebasis. For example, thedoseof ruxolitinib, or apharmaceutically acceptable salt thereof, is about 5, about 10, about 15, about 20, about 25, about 30, about 35, about 40, about 45, about 50, about 55, or 60 mg / day on a free base basis.

[0031] In some embodiments, the dose of ruxolitinib, or a pharmaceutically acceptable salt thereof, is about 2.5 mg BID toabout 30mgBIDona freebasebasis. Forexample, thedoseof ruxolitinib, or apharmaceutically acceptable salt thereof, is about 2.5, about 5, about 7.5, about 10, about 12.5, about 15, about 17.5, about 20, about 25, or about 30 mg BID on a free base basis.

[0032] In some embodiments, the pharmaceutically acceptable salt of ruxolitinib is ruxolitinib phosphate.

[0033] In some embodiments, 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methyl- sulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is selected from the phosphoric acid salt, dihydrochlorlic acid salt, hydrochloric acid salt, maleic acid salt, adipic acid salt, hydrobromic acid salt, (R)‑(‑)‑mandelic acid salt, salicylic acid salt, benzoic acid salt, benzenesulfonic acid salt, L-pyroglutamic acid salt, methanesulfonic acid salt, (1S)‑(+)‑10-camphorsulfonic acid salt, fumaric acid salt, sulfuric acid salt, L-tartaric acid salt, and D-tartaric acid salt of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfo- nyl)‑2H‑1,4-benzoxazin‑3(4H)‑one. In some embodiments, 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo 6 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 [2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one is a free base.

[0034] In some embodiments, the 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one includes one or more crystalline solid forms of an inhibitor of a BET protein. A more detailed discussion of Compound 1’s crystalline forms can be found in US Patent No. 10,626,114, which is incorporated herein by reference in its entirety, andbrieflydescribedherein. Typically, different crystalline formsof the same substance have different bulk properties relating to, for example, hygroscopicity, solubility, stability, and the like.

[0035] The crystalline solid forms of Compound 1 can include solvent such as water (e.g., a hydrated form) or be substantially free of water and solvent (e.g., forming an anhydrate). In some embodiments, the crystalline solid form is an anhydrate. In furtherembodiments, thecrystalline solid form ishydrated.Compound1canbeobtained inasolid crystalline form referred to asForm I, Form II, Form III, Form IV, FormV,FormVa,FormVI, FormVII, FormVIII, Form IX, FormX,Form XI, Form XII, Form XIII, Form XIV, and Form X, which are described in US Patent No. 10,626,114, which is incorporated herein by reference in its entirety.

[0036] Experimental data show that Form I is an anhydrate. Form I is characterized by its XRPD pattern and other solid state characteristics. In some embodiments, Form I has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.7, about 9.8, about 12.7, about 21.4, and about 23.3 degrees.

[0037] Experimental data show that Form II is an anhydrate. Form II is characterized by its XRPD pattern andother solid state characteristics. In some embodiments, Form II has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about about 6.7, about 9.5, about 10.5, about 14.8, about 16.2, about 17.0, about 18.8, and about 19.3 degrees.

[0038] In some embodiments, Form III has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 7.8, about 12.4, about 13.1, about 15.2, about 15.5, about 16.9, about 17.5, and about 20.3 degrees.

[0039] In some embodiments, Form IV has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 11.2, about 16.3, about 18.7, and about 22.1 degrees.

[0040] In some embodiments, Form V has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.2, about 8.5, about 14.1, about 16.3, about 17.1, about 18.9, about 19.8, about 21.8, and about 22.7 degrees.

[0041] In some embodiments, Form Va has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.7, about 16.5, about 17.3, about 19.9, and about 21.6 degrees.

[0042] In some embodiments, Form VI has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.5, about 9.6, about 11.4, about 12.1, about 13.5, about 14.5, about 15.2, about 17.1, about 17.7, about 18.1, about 19.2, and about 20.7 degrees.

[0043] In some embodiments, Form VII has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 9.9, about 12.2, about 14.8, about 15.7, about 17.0, about 17.5, and about 18.8 degrees.

[0044] In some embodiments, Form VIII has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.1, about 8.5, about 16.2, about 16.6, about 17.0, about 17.5, about 18.0, about 18.9, about 19.6, and about 20.1 degrees.

[0045] In some embodiments, Form IX has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.6, about 9.1, about 11.4, about 13.4, about 15.2, about 18.2, about 22.1, about 22.8, and about 23.9 degrees.

[0046] In some embodiments, Form X has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 14.9, about 15.3, about 15.8, about 17.0, about 17.7, about 18.3, and about 19.7 degrees.

[0047] In some embodiments, Form XI has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.9, about 12.8, about 18.0 about 21.5, about 22.6, and about 23.3 degrees.

[0048] In some embodiments, Form XII has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about5.6, about11.7, about13.8, about14.5,about16.9,about17.7, about18.7, about23.5,about24.6, about34.3, about 44.2, and 44.6 degrees.

[0049] In some embodiments, Form XIII has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 5.7, about 8.6, about 9.8, about 11.8, about 12.6, about 13.4, about 14.1, about 14.8, about 16.6, and about 19.1 degrees.

[0050] In some embodiments, Form XIV has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 4.0, about 11.2, about 11.9, about 14.1, about 14.8, and about 15.9 degrees.

[0051] In some embodiments, Form XV has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 7.4, about 9.6, about 12.4, about 13.4, about 15.5, about 16.9, about 17.7, about 19.0, about 19.5, about 20.6, and about 22.5 degrees.

[0052] The present application provides a method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and a therapeutically effectiveamount of ruxolitinib, or apharmaceutically acceptable salt thereof. In someembodiments, themyeloproliferative neoplasm is selected from polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis, chronic myelogenous leukemia (CML), chronic myelomonocytic leukemia (CMML), hypereosinophilic syndrome (HES), systemic 7 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 mast cell disease (SMCD), chronic neutrophilic leukemia (CNL), and chronic eosinophilic leukemia.

[0053] In some embodiments, the myeloproliferative neoplasm is polycythemia vera (PV).

[0054] In some embodiments, the myeloproliferative neoplasm is essential thrombocythemia (ET).

[0055] In some embodiments, the myeloproliferative neoplasm is primary myelofibrosis.

[0056] In some embodiments, the myeloproliferative neoplasm is myelofibrosis.

[0057] In some embodiments, ruxolitinib, or pharmaceutically acceptable salt thereof, and 2,2,4-trimethyl‑8‑(6- methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharma- ceutically acceptable salt thereof, are administered orally. In some embodiments, the ruxolitinib, or pharmaceutically acceptable salt thereof, is administered orally. In some embodiments, 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or apharmaceutically acceptable salt there- of, is administered orally.

[0058] In some embodiments, ruxolitinib, or pharmaceutically acceptable salt thereof, and 2,2,4-trimethyl‑8‑(6- methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharma- ceutically acceptable salt thereof, are administered simultaneously or sequentially.

[0059] The preceding embodiments are intended to be combined in any suitable combination as if the embodiments are multiplydependent claims (e.g., theembodiments related to the individual doses for ruxolitinib, theembodiments related to the individual doses for the BET protein inhibitor (Compound 1), the embodiments related to the salt forms, the embodiments related to the individual types of myeloproliferative neoplasms, and the embodiments related to oral administrationcanbecombined inanycombination). Thecombinationsarenot separately listedhereinmerely for thesake of brevity.

[0060] All compounds, and pharmaceutically acceptable salts thereof, can be found together with other substances such as water and solvents (e.g., hydrates and solvates) or can be isolated. When in the solid state, the compounds described herein and salts thereof may occur in various forms and may, e.g., take the form of solvates, including hydrates. The compounds may be in any solid state form, such as a polymorph or solvate, so unless clearly indicated otherwise, reference in the specification to compounds and salts thereof should be understood as encompassing any solid state form of the compound.

[0061] The phrase "pharmaceutically acceptable" is employed herein to refer to those compounds, materials, composi- tions and / or dosage forms which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of human beings and animals without excessive toxicity, irritation, allergic response, or other problem or complication, commensurate with a reasonable benefit / risk ratio.

[0062] The present invention also includes pharmaceutically acceptable salts of the compounds described herein. The term "pharmaceutically acceptable salts" refers to derivatives of the disclosed compounds wherein the parent compound is modified by converting an existing acid or base moiety to its salt form. Examples of pharmaceutically acceptable salts include, but arenot limited to,mineral or organic acid salts of basic residues suchasamines; alkali or organic salts of acidic residues such as carboxylic acids; and the like. The pharmaceutically acceptable salts of the present invention include the non-toxic salts of the parent compound formed, e.g., from non-toxic inorganic or organic acids. The pharmaceutically acceptable salts of the present invention can be synthesized from the parent compound which contains a basic or acidic moiety by conventional chemicalmethods.Generally, such salts canbepreparedby reacting the freeacid or base formsof these compounds with a stoichiometric amount of the appropriate base or acid in water or in an organic solvent, or in a mixture of the two; generally, non-aqueous media like ether, ethyl acetate, alcohols (e.g.,methanol, ethanol, iso-propanol, or butanol) or acetonitrile (MeCN) arepreferred. Lists of suitable salts are found inRemington’sPharmaceutical Sciences, 17thEd., (MackPublishingCompany,Easton, 1985), p. 1418,Bergeet al., J.Pharm.Sci., 1977, 66(1), 1‑19, and inStahl et al., Handbook of Pharmaceutical Salts: Properties, Selection, and Use, (Wiley, 2002). In some embodiments, the compounds described herein include the N-oxide forms.

[0063] The dosages described herein are on a free base basis. The phrase "on a free base basis" indicates that the amount of the comopund (e.g., ruxolitinib or salt thereof) in the dosage form is measured based on the molecular weight of the comopund free baseonly, evenwhen theactual active ingredient is a salt of the compoundhaving adifferentmolecular weight than the free base. For example, the conversion factor for ruxolitinib phosphate salt to free base is 0.7575.

[0064] The terms "individual" or "patient," used interchangeably, refer to any animal, including mammals, preferably mice, rats, other rodents, rabbits, dogs, cats, swine, cattle, sheep, horses, or primates, and most preferably humans.

[0065] The phrase "therapeutically effective amount" refers to the amount of active compound or pharmaceutical agent that elicits the biological or medicinal response in a tissue, system, animal, individual or human that is being sought by a researcher, veterinarian, medical doctor or other clinician.

[0066] The term "treating" or "treatment" refers to one or more of (1) inhibiting the disease; e.g., inhibiting a disease, condition or disorder in an individual who is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., arresting further development of the pathology and / or symptomatology); and (2) ameliorating the disease; e.g., ameliorating a disease, condition or disorder in an individual who is experiencing or displaying the pathology or symptomatology of the disease, condition or disorder (i.e., reversing the pathology and / or symptomatology) 8 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 such as decreasing the severity of disease. In one embodiment, treating or treatment includes preventing or reducing the risk of developing the disease; e.g., preventing or reducing the risk of developing a disease, condition or disorder in an individual who may be predisposed to the disease, condition or disorder but does not yet experience or display the pathology or symptomatology of the disease.

[0067] The term "BID" means two times a day.

[0068] The term "QD" means once a day. Additional Combinations

[0069] Compound 1 can be used in additional combination treatments where Compound 1 is administered in conjunction with other treatments such as the administration of one or more additional therapeutic agents. The additional therapeutic agents are typically thosewhich are normally used to treat the particular condition to be treated. Theadditional therapeutic agents can include, e.g., chemotherapeutics, anti-inflammatory agents, steroids, immunosuppressants, as well as Bcr-Abl, Flt‑3, RAF, FAK, and JAK kinase inhibitors for treatment of BET protein-associated diseases, disorders or conditions. The one or more additional pharmaceutical agents can be administered to a patient simultaneously or sequentially.

[0070] In some embodiments, Compound 1 can be used in combination with a therapeutic agent that targets an epigenetic regulator. Examples of epigenetic regulators include the histone lysine methyltransferases, histone arginine methyl transferases, histone demethylases, histone deacetylases, histone acetylases, and DNA methyltransferases. Histone deacetylase inhibitors include, e.g., vorinostat.

[0071] For treating cancer and other proliferative diseases, Compound 1 can be used in combination with chemother- apeutic agents, or other anti-proliferative agents. Compound 1 can also be used in combination with medical therapy such as surgery or radiotherapy, e.g., gamma-radiation, neutron beam radiotherapy, electron beam radiotherapy, proton therapy, brachytherapy, andsystemic radioactive isotopes.Examplesof suitable chemotherapeutic agents includeanyof: abarelix, aldesleukin, alemtuzumab, alitretinoin, allopurinol, altretamine, anastrozole, arsenic trioxide, asparaginase, azacitidine, bevacizumab, bexarotene, bleomycin, bortezombi, bortezomib, busulfan intravenous, busulfan oral, calus- terone, capecitabine, carboplatin, carmustine, cetuximab, chlorambucil, cisplatin, cladribine, clofarabine, cyclopho- sphamide, cytarabine, dacarbazine, dactinomycin, dalteparin sodium, dasatinib, daunorubicin, decitabine, denileukin, denileukin diftitox, dexrazoxane, docetaxel, doxorubicin, dromostanolone propionate, eculizumab, epirubicin, erlotinib, estramustine, etoposide phosphate, etoposide, exemestane, fentanyl citrate, filgrastim, floxuridine, fludarabine, fluor- ouracil, fulvestrant, gefitinib, gemcitabine, gemtuzumab ozogamicin, goserelin acetate, histrelin acetate, ibritumomab tiuxetan, idarubicin, ifosfamide, imatinib mesylate, interferon alfa 2a, irinotecan, lapatinib ditosylate, lenalidomide, letrozole, leucovorin, leuprolide acetate, levamisole, lomustine, meclorethamine, megestrol acetate, melphalan, mer- captopurine, methotrexate, methoxsalen, mitomycin C, mitotane, mitoxantrone, nandrolone phenpropionate, nelarabine, nofetumomab, oxaliplatin, paclitaxel, pamidronate, panitumumab, pegaspargase, pegfilgrastim, pemetrexed disodium, pentostatin, pipobroman, plicamycin, procarbazine, quinacrine, rasburicase, rituximab, ruxolitinib, sorafenib, streptozo- cin, sunitinib, sunitinib maleate, tamoxifen, temozolomide, teniposide, testolactone, thalidomide, thioguanine, thiotepa, topotecan, toremifene, tositumomab, trastuzumab, tretinoin, uracil mustard, valrubicin, vinblastine, vincristine, vinor- elbine, vorinostat, and zoledronate.

[0072] For treating cancer and other proliferative diseases, Compound 1 can be used in combination with ruxolitinib.

[0073] Compound 1 can be used in combination with one or more immune checkpoint inhibitors. Exemplary immune checkpoint inhibitors include inhibitors against immunecheckpointmolecules suchasCD27,CD28,CD40,CD122,CD96, CD73, CD47, OX40, GITR, CSF1R, JAK, PI3K delta, PI3K gamma, TAM, arginase, CD137 (also known as 4‑1BB), ICOS, A2AR, B7-H3, B7-H4, BTLA, CTLA‑4, LAG3, TIM3, VISTA, PD‑1, PD-L1 and PD-L2. In some embodiments, the immune checkpoint molecule is a stimulatory checkpoint molecule selected from CD27, CD28, CD40, ICOS, OX40, GITR and CD137. In some embodiments, the immune checkpoint molecule is an inhibitory checkpoint molecule selected from A2AR, B7-H3, B7-H4, BTLA, CTLA‑4, IDO, KIR, LAG3, PD‑1, TIM3, and VISTA. In some embodiments, the compounds provided herein can be used in combination with one or more agents selected from KIR inhibitors, TIGIT inhibitors, LAIR1 inhibitors, CD160 inhibitors, 2B4 inhibitors and TGFR beta inhibitors.

[0074] In some embodiments, the inhibitor of an immune checkpoint molecule is anti-PD1 antibody, anti-PD-L1 antibody, or anti-CTLA‑4 antibody.

[0075] In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of PD‑1, e.g., an anti-PD‑1 monoclonal antibody. In some embodiments, the anti-PD‑1 monoclonal antibody is nivolumab, pembrolizumab (also known as MK‑3475), pidilizumab, SHR‑1210, PDR001, or AMP‑224. In some embodiments, the anti-PD‑1 monoclonal antibody is nivolumab or pembrolizumab. In some embodiments, the anti-PD1 antibody is pembrolizumab.

[0076] In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of PD-L1, e.g., an anti-PD- L1 monoclonal antibody. In some embodiments, the anti-PD-L1 monoclonal antibody is BMS‑935559, MEDI4736, MPDL3280A (also known as RG7446), or MSB0010718C. In some embodiments, the anti-PD-L1 monoclonal antibody 9 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 is MPDL3280A or MEDI4736.

[0077] In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of CTLA‑4, e.g., an anti- CTLA‑4 antibody. In some embodiments, the anti-CTLA‑4 antibody is ipilimumab.

[0078] In someembodiments, the inhibitor of an immunecheckpointmolecule is an inhibitor of LAG3, e.g., ananti-LAG3 antibody. In some embodiments, the anti-LAG3 antibody is BMS‑986016 or LAG525.

[0079] In some embodiments, the inhibitor of an immune checkpoint molecule is an inhibitor of GITR, e.g., an anti-GITR antibody. In some embodiments, the anti-GITR antibody is TRX518 or MK‑4166.

[0080] In someembodiments, the inhibitor of an immunecheckpointmolecule is an inhibitor ofOX40, e.g., ananti-OX40 antibodyorOX40L fusionprotein. In someembodiments, theanti-OX40antibody isMEDI0562. Insomeembodiments, the OX40L fusion protein is MEDI6383.

[0081] Compound1canbeused in combinationwithoneormoreagents for the treatment of diseases suchas cancer. In some embodiments, the agent is an alkylating agent, a proteasome inhibitor, a corticosteroid, or an immunomodulatory agent. Examples of an alkylating agent include cyclophosphamide (CY), melphalan (MEL), and bendamustine. In some embodiments, theproteasome inhibitor is carfilzomib. In someembodiments, the corticosteroid is dexamethasone (DEX). In some embodiments, the immunomodulatory agent is lenalidomide (LEN) or pomalidomide (POM).

[0082] For treating autoimmune or inflammatory conditions, Compound 1 can be administered in combination with a corticosteroid such as triamcinolone, dexamethasone, fluocinolone, cortisone, prednisolone, or flumetholone.

[0083] For treating autoimmune or inflammatory conditions, Compound 1 can be administered in combination with an immune suppressant such as fluocinolone acetonide (Retisert®), rimexolone (AL‑2178, Vexol, Alcon), or cyclosporine (Restasis®).

[0084] For treating autoimmune or inflammatory conditions, Compound 1 can be administered in combination with one or more additional agents selected from Dehydrex™ (Holles Labs), Civamide (Opko), sodium hyaluronate (Vismed, Lantibio / TRBChemedia), cyclosporine (ST‑603,SirionTherapeutics), ARG101(T) (testosterone,Argentis), AGR1012(P) (Argentis), ecabet sodium (Senju-Ista), gefarnate (Santen), 15‑(s)‑hydroxyeicosatetraenoic acid (15(S)‑HETE), cevile- mine, doxycycline (ALTY‑0501, Alacrity), minocycline, iDestrin™ (NP50301, Nascent Pharmaceuticals), cyclosporine A (Nova22007, Novagali), oxytetracycline (Duramycin, MOLI1901, Lantibio), CF101 (2S, 3S, 4R, 5R)‑3, 4-dihy- droxy‑5‑[6‑[(3-iodophenyl)methylamino]purin‑9-yl]‑N-methyl-oxolane‑2-carbamyl, Can-Fite Biopharma), voclosporin (LX212 or LX214, Lux Biosciences), ARG103 (Agentis), RX‑10045 (synthetic resolvin analog, Resolvyx), DYN15 (Dyanmis Therapeutics), rivoglitazone (DE011, Daiichi Sanko), TB4 (RegeneRx), OPH‑01 (Ophtalmis Monaco), PCS101 (Pericor Science), REV1‑31 (Evolutec), Lacritin (Senju), rebamipide (Otsuka-Novartis), OT‑551 (Othera), PAI‑2 (University of Pennsylvania and Temple University), pilocarpine, tacrolimus, pimecrolimus (AMS981, Novartis), loteprednol etabonate, rituximab, diquafosol tetrasodium (INS365, Inspire), KLS‑0611 (Kissei Pharmaceuticals), dehy- droepiandrosterone, anakinra, efalizumab,mycophenolate sodium, etanercept (Embrel®), hydroxychloroquine, NGX267 (TorreyPines Therapeutics), or thalidomide.

[0085] In some embodiments, Compound 1 can be administered in combination with one or more agents selected from an antibiotic, antiviral, antifungal, anesthetic, anti-inflammatory agents including steroidal and non-steroidal anti-inflam- matories, and anti-allergic agents. Examples of suitable medicaments include aminoglycosides such as amikacin, gentamycin, tobramycin, streptomycin, netilmycin, and kanamycin; fluoroquinolones such as ciprofloxacin, norfloxacin, ofloxacin, trovafloxacin, lomefloxacin, levofloxacin, and enoxacin; naphthyridine; sulfonamides; polymyxin; chloramphe- nicol; neomycin; paramomycin; colistimethate; bacitracin; vancomycin; tetracyclines; rifampin and its derivatives ("ri- fampins"); cycloserine; beta-lactams; cephalosporins; amphotericins; fluconazole; flucytosine; natamycin; miconazole; ketoconazole; corticosteroids; diclofenac; flurbiprofen; ketorolac; suprofen; cromolyn; lodoxamide; levocabastin; napha- zoline; antazoline; pheniramine; or azalide antibiotic.

[0086] Other examples of agents, one or more of which a provided compound may also be combined with include: a treatment for Alzheimer’s Disease such as donepezil and rivastigmine; a treatment for Parkinson’s Disease such as L- DOPA / carbidopa, entacapone, ropinirole, pramipexole, bromocriptine, pergolide, trihexyphenidyl, and amantadine; an agent for treating multiple sclerosis (MS) such as beta interferon (e.g., Avonex® and Rebif®), glatiramer acetate, and mitoxantrone; a treatment for asthma such as albuterol and montelukast; an agent for treating schizophrenia such as zyprexa, risperdal, seroquel, and haloperidol; an anti-inflammatory agent such as a corticosteroid, such as dexametha- sone or prednisone, a TNF blocker, IL‑1 RA, azathioprine, cyclophosphamide, and sulfasalazine; an immunomodulatory agent, including immunosuppressive agents, such as cyclosporin, tacrolimus, rapamycin, mycophenolate mofetil, an interferon, a corticosteroid, cyclophosphamide, azathioprine, and sulfasalazine; a neurotrophic factor such as an acetylcholinesterase inhibitor, an MAO inhibitor, an interferon, an anti-convulsant, an ion channel blocker, riluzole, or ananti-Parkinson’sagent; anagent for treatingcardiovascular diseasesuchasabeta-blocker, anACE inhibitor, adiuretic, a nitrate, a calciumchannel blocker, or a statin; anagent for treating liver disease suchasacorticosteroid, cholestyramine, an interferon,andananti-viral agent; anagent for treatingblooddisorders suchasacorticosteroid, ananti-leukemicagent, or a growth factor; or an agent for treating immunodeficiency disorders such as gamma globulin.

[0087] In some embodiments, Compound 1 is administered in combination with a JAK kinase inhibitor (e.g., ruxolitinib, 10 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 tofacitinib, baricitinib,CYT387,GLPG0634, lestaurtinib, pacritinib, TG101348, or a JAK1-selective inhibitor), aPimkinase inhibitor (including inhibitors of oneormoreof PIM1,PIM2, andPIM3), aPI3 kinase inhibitor includingPI3K-delta selective and broad spectrum PI3K inhibitors, an MEK inhibitor, a cyclin dependent kinase inhibitor, a b-RAF inhibitor, an mTOR inhibitor, a proteasome inhibitor (e.g., bortezomib, carfilzomib), an HDAC-inhibitor (e.g., panobinostat, vorinostat), a DNA methyl transferase inhibitor, dexamethasone, melphalan, or an immunomodulator (e.g., lenolidomide, pomalidomide).

[0088] When more than one pharmaceutical agent is administered to a patient, they can be administered simulta- neously, sequentially, or in combination (e.g., for more than two agents). Compositions

[0089] The compounds can be administered in the form of pharmaceutical compositions. These compositions can be prepared in a manner well known in the pharmaceutical art, and can be administered by a variety of routes, depending upon whether local or systemic treatment is indicated and upon the area to be treated. Administration may be topical (including transdermal, epidermal, ophthalmic and to mucous membranes including intranasal, vaginal and rectal delivery), pulmonary (e.g., by inhalation or insufflation of powders or aerosols, including by nebulizer; intratracheal or intranasal), oral or parenteral. Parenteral administration includes intravenous, intraarterial, subcutaneous, intraperitoneal intramuscular or injection or infusion; or intracranial, e.g., intrathecal or intraventricular, administration. Parenteral administration can be in the form of a single bolus dose, or may be, e.g., by a continuous perfusion pump. Pharmaceutical compositions and formulations for topical administration may include transdermal patches, ointments, lotions, creams, gels, drops, suppositories, sprays, liquids and powders. Conventional pharmaceutical carriers, aqueous, powder or oily bases, thickeners and the like may be necessary or desirable.

[0090] The pharmaceutical compositions can contain, as the active ingredient, the compounds, or a pharmaceutically acceptable salt thereof, in combination with one or more pharmaceutically acceptable carriers (excipients). In some embodiments, the composition is suitable for topical administration. In making the compositions, the active ingredient is typically mixed with an excipient, diluted by an excipient or enclosed within such a carrier in the form of, e.g., a capsule, sachet, paper, or other container. When the excipient serves as a diluent, it can be a solid, semi-solid, or liquid material, which acts as a vehicle, carrier or medium for the active ingredient. Thus, the compositions can be in the form of tablets, pills, powders, lozenges, sachets, cachets, elixirs, suspensions, emulsions, solutions, syrups, aerosols (as a solid or in a liquid medium), ointments containing, e.g., up to 10% by weight of the active compound, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.

[0091] In preparing a formulation, the active compound can be milled to provide the appropriate particle size prior to combining with the other ingredients. If the active compound is substantially insoluble, it can be milled to a particle size of less than 200 mesh. If the active compound is substantially water soluble, the particle size can be adjusted by milling to provide a substantially uniform distribution in the formulation, e.g., about 40 mesh.

[0092] The compounds may be milled using known milling procedures such as wet milling to obtain a particle size appropriate for tablet formation and for other formulation types. Finely divided (nanoparticulate) preparations of the compounds of the invention can be prepared by processes known in the art see, e.g., WO 2002 / 000196.

[0093] Some examples of suitable excipients include lactose, dextrose, sucrose, sorbitol, mannitol, starches, gum acacia, calcium phosphate, alginates, tragacanth, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrroli- done, cellulose, water, syrup and methyl cellulose. The formulations can additionally include: lubricating agents such as talc, magnesium stearate and mineral oil; wetting agents; emulsifying and suspending agents; preserving agents such as methyl‑ and propylhydroxy-benzoates; and sweetening agents and flavoring agents. The compositions of the invention can be formulated so as to provide quick, sustained or delayed release of the active ingredient after administration to the patient by employing procedures known in the art.

[0094] In some embodiments, the pharmaceutical composition comprises silicified microcrystalline cellulose (SMCC) and at least one compound described herein, or a pharmaceutically acceptable salt thereof. In some embodiments, the silicified microcrystalline cellulose comprises about 98% microcrystalline cellulose and about 2% silicon dioxide w / w.

[0095] In some embodiments, the composition is a sustained release composition comprising at least one compound described herein, or a pharmaceutically acceptable salt thereof, and at least one pharmaceutically acceptable carrier. In some embodiments, the composition comprises at least one compound described herein, or a pharmaceutically acceptable salt thereof, and at least one component selected from microcrystalline cellulose, lactose monohydrate, hydroxypropyl methylcellulose and polyethylene oxide. In some embodiments, the composition comprises at least one compound described herein, or a pharmaceutically acceptable salt thereof, and microcrystalline cellulose, lactose monohydrate and hydroxypropyl methylcellulose. In some embodiments, the composition comprises at least one compound described herein, or a pharmaceutically acceptable salt thereof, and microcrystalline cellulose, lactose monohydrate and polyethylene oxide. In some embodiments, the composition further comprises magnesium stearate or silicon dioxide. In some embodiments, the microcrystalline cellulose is Avicel PH102™. In some embodiments, the lactose monohydrate is Fast-flo 316™. In some embodiments, the hydroxypropyl methylcellulose is hydroxypropyl 11 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 methylcellulose 2208 K4M (e.g., Methocel K4 M Premier™) and / or hydroxypropyl methylcellulose 2208 K100LV (e.g., Methocel K00LV™). In some embodiments, the polyethylene oxide is polyethylene oxide WSR 1105 (e.g., Polyox WSR 1105™).

[0096] The components used to formulate the pharmaceutical compositions are of high purity and are substantially free of potentially harmful contaminants (e.g., at least National Food grade, generally at least analytical grade, and more typically at least pharmaceutical grade). Particularly for human consumption, the composition is preferably manufactured or formulated under Good Manufacturing Practice standards as defined in the applicable regulations of the U.S. Food and Drug Administration. For example, suitable formulations may be sterile and / or substantially isotonic and / or in full compliance with all Good Manufacturing Practice regulations of the U.S. Food and Drug Administration.

[0097] The active compound may be effective over a wide dosage range and is generally administered in a therapeu- tically effective amount. It will be understood, however, that the amount of the compound actually administered will usually be determined by a physician, according to the relevant circumstances, including the condition to be treated, the chosen route of administration, the actual compound administered, the age, weight and response of the individual patient, the severity of the patient’s symptoms and the like.

[0098] The therapeutic dosage of a compound of the present invention can vary according to, e.g., the particular use for which the treatment ismade, themannerof administrationof thecompound, thehealthandconditionof thepatient, and the judgment of the prescribing physician. Theproportion or concentration of a compoundof the invention in a pharmaceutical composition can vary depending upon a number of factors including dosage, chemical characteristics (e.g., hydropho- bicity), and the route of administration.

[0099] For preparing solid compositions such as tablets, the principal active ingredient is mixed with a pharmaceutical excipient to form a solid preformulation composition containing a homogeneous mixture of a compound of the present invention. When referring to these preformulation compositions as homogeneous, the active ingredient is typically dispersed evenly throughout the composition so that the composition can be readily subdivided into equally effective unit dosage formssuchas tablets, pills andcapsules. This solid preformulation is thensubdivided intounit dosage formsof the typedescribedabovecontaining from,e.g.,about0.1 toabout 1000mgof theactive ingredient of thepresent invention.

[0100] The tablets or pills of the present invention can be coated or otherwise compounded to provide a dosage form affording the advantage of prolonged action. For example, the tablet or pill can comprise an inner dosage and an outer dosage component, the latter being in the form of an envelope over the former. The two components can be separated by an enteric layer which serves to resist disintegration in the stomach and permit the inner component to pass intact into the duodenumor to bedelayed in release.Avariety ofmaterials canbeused for suchenteric layersor coatings, suchmaterials including a number of polymeric acids and mixtures of polymeric acids with such materials as shellac, cetyl alcohol and cellulose acetate.

[0101] The liquid forms in which the compounds and compositions of the present invention can be incorporated for administration orally or by injection include aqueous solutions, suitably flavored syrups, aqueous or oil suspensions, and flavored emulsions with edible oils such as cottonseed oil, sesame oil, coconut oil, or peanut oil, as well as elixirs and similar pharmaceutical vehicles.

[0102] Compositions for inhalation or insufflation include solutions and suspensions in pharmaceutically acceptable, aqueous or organic solvents, or mixtures thereof, and powders. The liquid or solid compositions may contain suitable pharmaceutically acceptable excipients asdescribed supra. In someembodiments, the compositionsareadministeredby the oral or nasal respiratory route for local or systemic effect. Compositions can be nebulized by use of inert gases. Nebulized solutions may be breathed directly from the nebulizing device or the nebulizing device canbe attached to a face mask, tent, or intermittent positive pressure breathing machine. Solution, suspension, or powder compositions can be administered orally or nasally from devices which deliver the formulation in an appropriate manner.

[0103] Topical formulations can contain one or more conventional carriers. In some embodiments, ointments can contain water and one or more hydrophobic carriers selected from, e.g., liquid paraffin, polyoxyethylene alkyl ether, propylene glycol, white Vaseline® (petroleum jelly) and the like. Carrier compositions of creams can be based on water in combination with glycerol and one or more other components, e.g., glycerinemonostearate, PEG-glycerinemonostearate and cetylstearyl alcohol. Gels can be formulated using isopropyl alcohol and water, suitably in combination with other components such as, e.g., glycerol, hydroxyethyl cellulose and the like.

[0104] The amount of compound or composition administered to a patient will vary depending upon what is being administered, the purpose of the administration, such as prophylaxis or therapy, the state of the patient, the manner of administration and the like. In therapeutic applications, compositions can be administered to a patient already suffering from a disease in an amount sufficient to cure or at least partially arrest the symptoms of the disease and its complications. Effective doses will depend on the disease condition being treated as well as by the judgment of the attending clinician depending upon factors such as the severity of the disease, the age, weight and general condition of the patient and the like.

[0105] The compositions administered to a patient can be in the form of pharmaceutical compositions described above. Thesecompositionscanbesterilizedbyconventional sterilization techniques, ormaybesterile filtered.Aqueoussolutions 12 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 canbepackaged foruseas is, or lyophilized, the lyophilizedpreparationbeingcombinedwithasterileaqueouscarrierprior to administration. The pHof the compound preparations typically will be between 3and11,more preferably from 5 to 9and most preferably from 7 to 8. It will be understood that use of certain of the foregoing excipients, carriers or stabilizers will result in the formation of pharmaceutical salts. Kits

[0106] The present application also includes pharmaceutical kits useful, which include one or more containers containing a pharmaceutical composition comprising a therapeutically effective amount of the compound, or any of the embodiments thereof. Such kits can further include one or more of various conventional pharmaceutical kit components, such as, e.g., containers with one or more pharmaceutically acceptable carriers, additional containers, etc., as will be readily apparent to those skilled in the art. Instructions, either as inserts or as labels, indicating quantities of the components to be administered, guidelines for administration, and / or guidelines for mixing the components, can also be included in the kit.

[0107] The invention will be described in greater detail by way of specific examples. The following examples are offered for illustrative purposes, and are not intended to limit the invention in any manner. Those of skill in the art will readily recognize a variety of noncritical parameters which can be changed or modified to yield essentially the same results. The compounds of the Examples have been found to be BET protein-kinase inhibitors according to at least one assay described herein. EXAMPLES Example 1: Characterization of Compound 1 in Xenograft Models of JAK2V617F-MPN / AML Compound and Formulations:

[0108] Compound 1 was used in these studies and was manufactured at Incyte Corporation. Ruxolitinib was used in these studies and was manufactured by Wilmington PharmaTech. Methods:

[0109] Female SCID mice at approximately 5‑6 weeks old were subcutaneously inoculated with 107 SET‑2 cells in matrigel. Dosing beganwhen tumor size reachedapproximately 200mm3.Dosing for these studieswasperformedbyoral gavage.

[0110] Forefficacy studies,measurementsof tumorswere takenevery 2 to 3daysusing adigital caliper. Tumor volumes were calculated using the formula: Volume = (length x width2) / 2 where width was the smaller dimension. Body weights were also monitored.

[0111] Animalswere sacrificed (1) at endof study, (2) if tumor size reached10%of total bodyweight, or (3) if 20%of body weight is lost.

[0112] Statistical analyses were performed using Prism Graphpad software. All comparisons were done using 2-way ANOVA with p< 0.05 deemed significant. Efficacy of Compound 1 in the JAK2 V617F Expressing HEL Xenograft Model of Erythroleukemia

[0113] The purpose of this study was to examine the ability of the BET inhibitor Compound 1 inhibit tumor growth in an MPN-like AML model expressing the JAK2 V617F mutation. Female SCID mice were subcutaneously inoculated with 107 HEL cells in matrigel. When tumors reached approximately 160 mm3, mice (n = 10 per group) were dosed orally with Compound 1 for 14 days as follows: 1. BID at either 1 or 3 mg / kg, or vehicle control 2. QD at 3, 10, or 30 mg / kg

[0114] As shown in FIG. 1A, administration of Compound 1 inhibited tumor growth in a dose-related manner, and the mean tumor volumes from each of the Compound 1 treatment groups were statistically significantly reduced from that of the vehicle group (p<0.02 for eachgroup, 2-wayANOVA). The30mg / kgQDdosewas themost efficaciousand resulted in one statistical tumor regression. The data indicate that dosing Compound 1 on a BID regimen could be considered, but is unnecessary,asQDdosing isaseffectiveasBIDdosing in thismodel.All doseswere toleratedasdeterminedby the lackof 13 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 body weight loss as shown in FIG. 1B. Efficacy from combination of Compound 1 and ruxolitinib in the SET‑2 xenograft model

[0115] Female SCID mice were subcutaneously inoculated with 107 SET‑2 cells in matrigel. When tumors reached approximately 175 mm3, mice (n = 8 per group) were dosed orally for 14 days as follows: 1. Vehicle control, or ruxolitinib at 30 mg / kg BID 2. Compound 1 at 10 mg / kg QD 3. Combination of ruxolitinib and Compound 1

[0116] Compound 1 and ruxolitinib were dosed sub-optimally for an optimal read-out of possible combinatorial effects. As seen in FIG. 2A, both monotherapies significantly inhibited tumor growth versus vehicle controls (p < 0.02 for each, 2- way ANOVA). In addition, the combination of Compound 1 and ruxolitinib gave enhanced and significant (p < 0.007 for combination vs. both single agent arms; 2-way ANOVA) efficacy relative to expected outcomes based on single agent efficacies. Approximately 15% body weight loss did occur in the combination group (FIG. 2B), but this nadir was within the limits of tolerability. Efficacy from combination of Compound 1 and ruxolitinib in the SET‑2 xenograft model

[0117] Female SCID mice were subcutaneously inoculated with 107 SET‑2 cells in matrigel. When tumors reached approximately 150 mm3, mice (n = 8 per group) were dosed orally for 11 days as follows: 1. Vehicle control, or ruxolitinib at 60 mg / kg BID 2. Compound 1 at 10 mg / kg QD 3. Combination of ruxolitinib and Compound 1

[0118] In this study, ruxolitinib was administered at a higher dose than in 1.2, resulting in a significant tumor growth inhibition as a monotherapy (FIG. 3A). Compound 1 administration with ruxolitinib did not increase the tumor growth inhibition seen with single agent ruxolitinib or single agent Compound 1. All doses were tolerated, although the combination of Compound 1 and ruxolitinib did result in body weight loss (FIG. 3B approximately 12%) within the allowable limit.

[0119] The BET inhibitor Compound 1 is efficacious at tolerable doses in two models of JAK2V617FAML. Combination of Compound 1 with a sub-optimal dose of the JAK1 / 2 inhibitor ruxolitinib (30 mg / kg BID) results in a significant increase in tumor growth inhibition over monotherapies. These data demonstrate the potential for BET inhibitors in combination with JAK inhibitors in JAK2V617F driven myeloid neoplasms. Example 2: Efficacy of Compound 1 in Combination with Ruxolitinib in a Mouse Model of MPLW515L-Driven MPN Compound and Formulations:

[0120] Compound 1 and ruxolitinib were used in these studies and were manufactured by Wilmington PharmaTech. Methods:

[0121] At one week prior to bone marrow transplant, eight week old female Balb / c mice (Charles River Laboratories) were injected once intraperitoneally with 150 mg / kg 5-fluorouracil. Five days later, these mice were sacrificed, and bone marrowwasharvestedbyaspirationof femurs.Redbloodcellswere lysedusingBDPharmaLysebuffer (BDBiosciences), then washed with PBS. The remaining marrow was plated in 10% FBS / RPMI overnight in standard cell culture incubation. The following day, the bone marrow was infected with ecotropic retrovirus (MSCV puromycin backbone) expressing the MPLW515L gene by adding 1 ml virus to 106 cells, then centrifugation at 1800 rpm, 37°C, 90 minutes in 6 well plates. Following centrifugation, infected cells were kept in the incubator until the following day when they were injected into recipients. 14 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55

[0122] Bone marrow of recipient mice was ablated in eight week old Balb / c mice using 50 mg / kg 5-fluorouracil five days prior to transplantationwithBalb / cbonemarrowexpressingMPLW515Lvia retroviral infection (approximately2x105cells per recipient mouse). Blood counts were performed 7 days after transplant and 4 cohorts (n=10 per cohort) with equal meanplatelet numberweregenerated for treatment.Dosing commencedonday8post implant and continued for 13days. At day 14 post implant, mice were sacrificed, blood collected by cardiac puncture, and spleens weighed as a surrogate for disease burden.

[0123] Blood was collected via by orbital sinus once weekly, and complete blood counts (CBCs) were determined by hematology instrumentation (Abaxis, model HM5).

[0124] Statistical analyses wereperformed usingPrism Graphpad software. All comparisonswere doneusing unpaired t tests with p < 0.05 deemed significant. Efficacy of Compound 1 in combination with ruxolitinib in the MPLW515L model of mouse MPN

[0125] Female Balb / c mice were transplanted with MPLW515L expressing bone marrow in order to assess the ability of the BET inhibitor Compound 1 to improve upon the activity of ruxolitinib alone in a preclinical MPN model. On day 7 post transplant, mice were randomized into cohorts for dosing based on platelet levels, as determined by CBC. The following day oral dosing commenced, with mice receiving Compound 1 at 10 mg / kg QD, ruxolitinib at 60 mg / kg BID, the combination of Compound 1 and ruxolitinib, or vehicle control. Dosing proceeded for 14 days, after which mice were bled for CBC, and spleens were harvested and weighed as a surrogate for disease burden. Two vehicle treated mice died before end of study on day 14 post dosing, one on day 12 of dosing and one on day 13 post dosing.

[0126] Whileeachsingleagentwasable to reducespleensize, thecombinationgave themostefficacy.Mice treatedwith theCompound1 / ruxolitinib combination had spleens thatwere significantly smaller than thoseof either single agent alone (FIG. 4A).Ruxolitinib had amoreprofoundeffect onwhite blood cell count reduction thanCompound1, and therewas little difference between WBC counts from ruxolitinib treated mice and mice receiving combination therapy (FIG. 4B).

[0127] Statistics were determined by unpaired t tests performed using Prism Graphpad software (** p < 0.01; ****p < 0.0001).

[0128] At the doses chosen for this study, both Compound 1 and ruxolitinib were able to slow the expansion of white blood cells and suppress splenomegaly brought onbyMPLW515Lexpressing bonemarrow.Combination ofCompound1 with ruxolitinib resulted in a significant decrease in disease burden over either monotherapy as measured by spleen weight. These data demonstrate the potential for BET inhibitors in combination with JAK inhibitors in myeloproliferative neoplasms. Example 3: Statistical Demonstration of Synergy Between Compound 1 and Ruxolitinib

[0129] The synergy between Compound 1 and ruxolitinib was assessed using the Chou-Talalay equation (see Chou, "DrugCombinationStudiesandTheirSynergyQuantificationUsing theChou-TalalayMethod"CancerRes; 70(2) January 15, 2010, incorporated by reference herein). Synergy depicts a greater effect with the combination than what would be expectedbasedonhow the individual compoundsareworking. InTable1, therearedata for the twoexperimentsdescribed herein. The first dasa set listed is the MPLW515L model, spleen weights are used as disease progression markers (i.e., bigger spleen = worse disease). MPL SPL depicts the sizes of the spleens in grams from the MPLW515L murine MPN model. Fa shows the fraction affected which is the percentage change in comparison to the vehicle. Expected row shows the expected effect of the combination based on the Chou-Talalay synergy index. Since the number is smaller than the effect in the fraction affected, this demonstrates a larger effect than expected from combining Compound 1 and ruxolitinib demonstrating that synergy isoccurringbetween the twocompoundsgivinganunexpectedeffect. Thesecondexperiment is SET‑2 which is the JAK2V617F expressing cell line used in a xenograft tumor model. Fa for SET‑2 are the tumor growth inhibition levels for each dose used and the combination. Again the expected value is smaller than the actual effect demonstrating an unexpected synergy. In both experiments, the Chou-Talalay equation gave us a value that was smaller than the effect seen with the combinations of ruxolitinib plus Compound 1, this is interpreted as the combination being synergistic in each experiment. Table 1 Vehicle Ruxolitinib 60mg / kg BID Compound 1 10mg / kg QD Combination MPL SPL 0.812975 0.3367 0.3611 0.0965 Fa 0.585842123 0.5558289 0.881300163 Expected 0.81604304 15 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 (continued) Vehicle Ruxolitinib 60mg / kg BID Compound 1 10mg / kg QD Combination SET‑2 Fa 0.44 0.24 0.71 Expected 0.5744 Example 4: Clinical Study Protocol of Compound 1 as Monotherapy in Participants with Myelofibrosis Study Design

[0130] This is a Phase 1, open-label, 2-part study of Compound 1 as monotherapy in participants with relapsed or refractory myelofibrosis (for further details see "Safety and Tolerability Study of INCB057643 in Participants With Myelofibrosis", ClinicalTrials.gov Identifier: NCT04279847, which is incorporated herein by reference in its entirety). INCB057643 is also referred to as Compound 1 in the present disclosure. Participants include those who have received at least 1 line of prior therapy including ruxolitinib and have no further available therapy known to provide clinical benefit and with a risk category of intermediate‑2 or high according to DIPSS. Participants will receive 4 mg QD of Compound 1 on a continuous basis.

[0131] The study will be conducted in 2 parts. Part 1 will evaluate initial safety and tolerability of 4 mg QD of Compound 1 in participants with relapsed or refractory myelofibrosis. Study drug will be self-administered once daily every day for 28 days, which is 1 cycle. Participants will continue taking study drug as long as benefit is derived and discontinuation criteria is not met.

[0132] If the starting dose of 4 mg QD is deemed tolerable in Part 1, the starting dose in Part 2 will be 4 mg QD; if not, the starting dose in Part 2 will be 2 mg QD. Part 2 will administer Compound 1 as monotherapy as well.

[0133] Throughout the whole study, in both Part 1 and Part 2, AEs will be continuously monitored. If the cumulative incidence of Grade 3 or 4 drug-related AEs occurs in > 40% of participants, the study will be stopped. Likewise, if there is more than 1 fatal drug-related event, the study will be stopped. The study may only be restarted after discussion with the FDA. Pharmacokinetics of Compound 1 as Monotherapy

[0134] Theclinical PKofCompound1 inparticipantswith advancedmalignancies (solid andhematologicmalignancies) were evaluated in a Phase 1 / 2, open-label, dose-escalation / dose-expansion, safety and tolerability study. Participants received continuous QD doses of Compound 1. As of the data cutoff date (23 SEP 2019), 25, 101, and 8 participants who received8mg, 12mg, and16mg, respectively, acrossPart 1 andPart 2 (Compound1 treated asmonotherapy) havebeen assessed for PK. The PK parameters of parent Compound 1 are summarized in Table 2 and Table 3 (see FIG. 5). Table 2 presents a summary of Compound 1 pharmacokinetic parameters for Compound 1 as monotherapy (part 1 and 2) at cycle 1day1.Table3presentsasummaryofCompound1pharmacokinetic parameters forCompound1asmonotherapy (part 1 and 2) at steady-state (cycle 1 day 8). Table 2 Analyte Dose Participant(s) Cmax (nM) Tmax (h) AUClast (h*nM) Compound 1 (Parent) 8 mg N = 12 201 ± 83.6 2.00 2310 ± 1120 188 (38.8) (1.00, 24.0) 2090 (50.1) 12 mg N = 99 266 ± 94.7 0.50 2740 ± 1090 249 (39.1) (2.00, 8.00) 2550 (39.5) 16 mg N = 7 343 ± 93.2 2.00 3370 ± 1060 329 (34.5) (2.00, 4.00) 3210 (34.3) Note: Values are presented in the format of mean ± SD and geometric mean (CV%) except that Tmax is reported as median (range).

[0135] Blood samples for the determination of plasma concentrations of Compound 1 in Parts 1 and 2 were collected at predoseonCycle 1Day1,Cycle 1Day2, andCycle 1Day8, andat 0.5, 1, 2, 4, 6, and8hours onCycle 1Day1andCycle 1 Day 8. The plasma samples of Compound 1 were assayed by a validated LC-MS / MS method. 16 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55

[0136] With multiple-dose administration in the fasted state, Compound 1 plasma concentrations attained the peak values (Cmax) typically at 1‑2 hours (median Tmax) postdose, and subsequently exhibited a monophasic decay, with a steady-state geometric mean t½ of approximately 10 hours that was not dose-dependent. The steady-state was predicted to be achieved after 2 days with once daily dosing, based on an effective half-life of 10 hours. There is minimal accumulation of Compound 1 exposure (< 10%) by comparing steady-state AUC0‑24 to first dose AUC0‑24. Within the dose range of 8 to 16mg QD, increases in theCompound 1 steady-stateCmax and AUC0‑24 were proportional to dose, that is,Compound1exhibitedapproximately linearpharmacokineticsover thedose rangestudied.ANOVAofdosenormalized PK parameters (Cmax,ss and AUCss,0‑24) using overall test or pairwise comparisons (see Table 2) demonstrated that dose normalized Cmax,ss or AUCss,0‑24 was not statistically significantly different (P > 0.05) between or across the doses. Compound 1 exhibited a low steady-state oral clearance with geometric mean CLss / F of 9.92‑10.7 L / h and moderate volume of distribution with geometric mean Vz / F of 138‑197 L. At a dose of 12 mg QD, the geometric mean (CV%) of t½, steady-state Cmax, and AUC0‑24 were 9.17 h (49.7%), 272 nM (40.9%), and 2740 h*nM (43.2%), respectively.

[0137] The estimated steady-state AUC0‑24 of Compound 1 is 457 h*nM and 913 h*nM for 2 mg QD and 4 mg QD, respectively, based on linear PK extrapolation from 12 mg QD geometric mean AUCss,0‑24. The simulated PK data of Compound 1 at 4 mg QD are presented in FIG. 7. Pharmacodynamics for the Treatment of Compound 1 as Monotherapy

[0138] Pharmacodynamic analysis was performed using an ex vivo assay measuring protein levels of cMyc, a BRD4 target gene, in KMS12BM cells, which were spiked into participant plasma samples collected at predose and various postdose timepoints. In preliminary PD analysis after 8 mg, 12 mg, and 16 mg of oral administration, Compound 1 demonstrated inhibition of total cMyc protein expression with maximal inhibition occurring between 1 and 4 hours. The average cMyc inhibition at steady state (Day 8, from predose to 8 hours) was 30%, 44%, and 65% for the dose of 8 mg (n = 11), 12 mg (n = 51), and 16 mg (n = 7), respectively. Maximum individual peak inhibition of total cMyc protein expression ranged from 16% to 77% at 8 mg, 20% to 92% at 12 mg, and 61% to 97% at 16 mg dose level. Inhibition of cMyc was reduced to < 10% at trough (Cycle 1 Day 8 predose) in the 8-mg and 12-mg cohorts and ~30% in the 16-mg cohort. A composite PK PD curve was plotted for 70 participants, and an IC50 value of 202.4 nM was determined by nonlinear regression curve fitting (see FIG. 6). The ex vivo IC50 coverage over steady-state concentrations of Compound 1 are shown in FIG. 7. Relationship of Compound 1 Steady-State AUC and Treatment-Emergent Adverse Events

[0139] The selected clinical safety endpoints as deemed appropriate (eg, frequently occurring TEAEs (treatment- emergent adverse events), incident rate > 20% for all AEs (adverse events) and clinically notable adverse events) were used toevaluateexposure-safety relationships. Thesafetydata froma total of 106participants (includingbothsolid tumors andhematologicmalignancies) receivingmonotherapy treatmentofCompound1 (n=10at 8mg,n=89at12mg,andn=8 at16mg) inParts1and2wereused foranalysis. Frequently occurringTEAEs (all gradesandcausality) in participantswho received at least 1 dose of study drug included nausea (47.9%), fatigue (45.5%), decreased appetite (35.5%), thrombo- cytopenia (28.9%), vomiting (28.1%), anemia (27.3%), diarrhea (27.3%), constipation (20.7%), anddysgeusia (19.8%)as well as clinically notable AEs including hyperglycemia (17.4%), epistaxis (9.9%), INR increased (8.5%), and gastro- intestinal hemorrhage (1.9%).

[0140] No statistically significant correlation was identified for steady-state Compound 1 AUC and any TEAEs or clinically notable AEs evaluated in this analysis, except hyperglycemia (p-value < 0.05). FIG. 8 shows model-predicted versusobserved relationshipofCompound1steadystateAUCandprobability ofhyperglycemia.Thepredictedprobability of hyperglycemia incidence at dose of 2 mg and 4 mg is 8.0% and 9.2%, respectively, based on AUC and hyperglycemia relationship (Note: Open squares represent first (893‑2035 h*nM), second (2074‑2605 nM), third (2622‑3564 nM), and fourth (3606‑9788 nM) quartiles of Compound 1 AUCss,0‑24.). Lack of correlation between Compound 1 AUCss,0‑24 and TEAEs could be due to the narrow dose range (8, 12, or 16 mg) explored in this study and small sample sizes in the 8-mg and 16-mg groups of the study (the majority of participants received 12 mg).

[0141] Table 4 lists the estimated parameters for Compound 1 AUC and Grade 3 INR (international normalized ratio) increases and Grade 4 gastrointestinal hemorrhage. Two Grade 3 events of INR occurred in participants concomitantly taking warfarin. The steady state AUC of these 2 participants were 6990 h*nM and 9770 h*nM receiving Compound 112 mg QD and16 mg QD, respectively, and theAUCs weremuch higher than the geometricmean steady-stateAUC in 12 mg QD (2740 h*nM) and 16 mg QD (3610 h*nM), respectively.

[0142] Two Grade 4 events of gastrointestinal hemorrhage were observed in 2 participants receiving 12 mg QD dose, and thesteady-stateAUCof these2participantswere3470h*nMand3610h*nM, respectively, and theAUCswereslightly higher than geometric mean steady-state AUC in 12 mg QD. 17 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 Table 4 Participants Adverse Event Grade Dose (mg) AUCss,0‑24 (h*nM) 1011 Gastrointestinal hemorrhage 4 12 3470 9002 Gastrointestinal hemorrhage 4 12 2150 12005 INR increased 3 12 6990 16001 INR increased 3 16 9790 Example 5: Efficacy of Compound 1 in Combination with Ruxolitinib in Participants with Myelofibrosis

[0143] Preliminary efficacy (spleen length and / or volume reduction) was observed in 2 of 3 myelofibrosis participants treated in the study referenced herein (for further details see "Safety and Tolerability Study of INCB057643 in Participants With Myelofibrosis", ClinicalTrials.gov Identifier: NCT04279847, which is incorporated herein by reference in its entirety). All three participants were administered ruxolitinib for 6 months prior. A first participant and a second participant had relapsedmyelofibrosiswhichwas treatedwithCompound1monotherapy.A thirdparticipantwasbeing treatedwith single- agent ruxolitinib but having suboptimal responses. In reaction to the suboptimal responses, the third participant was then treated with Compound 1 in combination with ruxolitinib.

[0144] Thefirst participant receivedCompound1monotherapy12mgQDandhadaspleen length reductionof92.5%by spleen palpation (nadir compared to the baseline). The second participant with relapsed myelofibrosis received Compound 1 monotherapy 8 mg QD. The second participant on the lower dose of Compound 1 resulted in only disease progression, and the second participant stayed on study for 24 days. The third participant received Compound 1 8 mg QD in combination with ruxolitinib and experienced a 77% spleen length reduction by spleen palpation and a 44% spleen volume reductionby imaging (nadir compared to thebaseline). Toput thepositive results of thefirst and third participants in context, when a ruxolitinib monotherapy was employed in clinical trials about 35% reduction in spleen size was observed (for further details seeVerstovsekS.,MorganG. "Results ofCOMFORT‑ I, a randomizeddouble-blind phase III trial of JAK 1 / 2 inhibitor INCB18424 (424) vs placebo (PB) for patients with myelofibrosis (MF)", Abstract #6500. 2011 American Society of Clinical Oncology Annual Meeting, which is incorporated herein by reference in its entirety).

[0145] Further preliminary efficacy of a BET inhibitor (CPI‑0610) in myelofibrosis participants was also reported in the MANIFESTstudy (NCT02158858;Hoffman et al 2019,Mascarenhas et al 2019,which is incorporated herein by reference in its entirety). The enrolled myelofibrosis participants were either refractory, intolerant, or ineligible for ruxolitinib (in the CPI‑0610 monotherapy cohort), or were receiving ruxolitinib but experiencing suboptimal response or myelofibrosis progression (in the CPI‑0610 and ruxolitinib combination cohort). The participants experienced best spleen volume reduction which ranged from 6%‑44% in 10 evaluable participants (monotherapy and combination combined).

[0146] Variousmodificationsof the invention, inaddition to thosedescribedherein,will beapparent to thoseskilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims. Each reference, includingwithout limitationall patent, patentapplications, andpublications, cited in thepresentapplication is incorporated herein by reference in its entirety. Numbered clauses:

[0147] 1. A method of treating a myeloproliferative neoplasm in a patient in need thereof, comprising administering to said patient 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-ben- zoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof; and ruxolitinib, or a pharmaceutically acceptable salt thereof. 2. The method of clause 1, wherein the ruxolitinib, or a pharmaceutically acceptable salt thereof, is ruxolitinib phosphate. 3. The method of clause 1 or 2, wherein 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one is a solid form. 4. The method of clause 3, wherein the solid form is an anhydrate. 5. The method of clause 3, wherein the solid form is Form I. 18 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 6. The method of clause 5, wherein Form I has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 8.7, about 9.8, about 11.6, about 12.7, about 14.7, about 15.7, about 20.0, about 21.4, about 23.3, and about 27.1 degrees. 7. The method of clause 3, the solid form having Form II. 8. The method of clause 7, wherein Form II has one or more characteristic XRPD peaks, in terms of 2-theta, selected from about 6.7, about 9.5, about 10.5, about 14.8, about 16.2, about 17.0, about 18.8, and about 19.3 degrees. 9. Themethodof anyoneof clauses1‑8,wherein thedoseof ruxolitinib, or a pharmaceutically acceptable salt thereof, is about 5 mg / day to about 60 mg / day. 10. The method of any one of clauses 1‑8, wherein the dose of ruxolitinib, or a pharmaceutically acceptable salt thereof, is about 2.5 mg BID to about 30 mg BID. 11. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2 mg / day to about 20 mg / day. 12. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2 mg / day to about 18 mg / day. 13. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2 mg / day to about 12 mg / day. 14. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 4 mg / day to about 8 mg / day. 15. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2, about 4, about 6, about 8, about 10, about 12, about 14, about 16, about 18, or about20mg / dayona free base basis. 16. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 2 mg / day on a free base basis. 17. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 4 mg / day on a free base basis. 18. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 6 mg / day on a free base basis. 19. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 8 mg / day on a free base basis. 20. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is about 10 mg / day on a free base basis. 21. The method of any one of clauses 1‑10, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H- pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt 19 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 thereof, is about 12 mg / day on a free base basis. 22. The method of any one of clauses 1‑21, wherein the myeloproliferative neoplasm is selected from polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis, chronic myelogenous leukemia (CML), chronic myelomonocytic leukemia (CMML), hypereosinophilic syndrome (HES), systemicmast cell disease (SMCD), chronic neutrophilic leukemia (CNL), and chronic eosinophilic leukemia. 23. The method of clause 22, wherein the myeloproliferative neoplasm is polycythemia vera (PV). 24. The method of clause 22, wherein the myeloproliferative neoplasm is essential thrombocythemia (ET). 25. The method of clause 22, wherein the myeloproliferative neoplasm is primary myelofibrosis. 26. Themethodof anyoneof clauses1‑25,wherein ruxolitinib, or pharmaceutically acceptable salt thereof, and2,2,4- trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxa- zin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, are administered orally. 27. Themethodof anyoneof clauses1‑26,wherein ruxolitinib, or pharmaceutically acceptable salt thereof, and2,2,4- trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxa- zin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, are administered simultaneously or sequentially. Claims 1. A solid form of a compound which is 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4- yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, for use in a method of treating a myeloproliferative neoplasm in a patient in need thereof, the method comprising administering to said patient the compound, or a pharmaceutically acceptable salt thereof; and ruxolitinib, or a pharmaceutically acceptable salt thereof; wherein the solid form is Form III, Form IV, Form V, Form Va, Form VI, Form VII, Form VIII, Form IX, Form X, Form XI, Form XII, Form XIII, Form XIV, or Form XV. 2. Ruxolitinib, or a pharmaceutically acceptable salt thereof, for use in a method of treating a myeloproliferative neoplasm in a patient in need thereof, the method comprising administering to said patient ruxolitinib, or a pharmaceutically acceptable salt thereof; and a solid form of a compound which is 2,2,4-trimethyl‑8‑(6-methyl‑7- oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceuti- cally acceptable salt thereof; wherein the solid form is Form III, Form IV, Form V, Form Va, Form VI, Form VII, Form VIII, Form IX, Form X, Form XI, Form XII, Form XIII, Form XIV, or Form XV. 3. Acombinationofasolid formofacompoundwhich is2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c] pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof, for use in amethodof treatingamyeloproliferative neoplasm in a patient in need thereof, the method comprising administering to said patient the compound, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof; wherein the solid form is Form III, Form IV, Form V, Form Va, Form VI, Form VII, Form VIII, Form IX, Form X, Form XI, Form XII, Form XIII, Form XIV, or Form XV. 4. The solid form for use according to claim 1, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to claim 2, or the combination for use according to claim 3, wherein the ruxolitinib, or a pharmaceutically acceptable salt thereof, is ruxolitinib phosphate. 5. The solid form for use according to claim 1 or 4, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to claim 2 or 4, or the combination for use according to claim 3 or 4, wherein the solid form is: a) Form III having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 7.8, 12.4, 13.1, 15.2, 15.5, 16.9, 17.5 and 20.3 degrees; b) Form IV having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 11.2, 16.3, 20 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 18.7, and 22.1 degrees; c)FormVhavingoneormorecharacteristicXRPDpeaks, in termsof2-theta (±0.2°), selected from8.2,8.5, 14.1, 16.3, 17.1, 18.9, 19.8, 21.8, and 22.7 degrees; d) Form Va having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.7, 16.5, 17.3, 19.9, and 21.6 degrees; e) Form VI having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.5, 9.6, 11.4, 12.1, 13.5, 14.5, 15.2, 17.1, 17.7, 18.1, 19.2, and 20.7 degrees; f) Form VII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 9.9, 12.2, 14.8, 15.7, 17.0, 17.5, and 18.8 degrees; g) Form VIII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.1, 8.5, 16.2, 16.6, 17.0, 17.5, 18.0, 18.9, 19.6, and 20.1 degrees; h) Form IX having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.6, 9.1, 11.4, 13.4, 15.2, 18.2, 22.1, 22.8, and 23.9 degrees; i) Form X having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 14.9, 15.3, 15.8, 17.0, 17.7, 18.3, and 19.7 degrees; j) Form XI having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.9, 12.8, 18.0 21.5, 22.6, and 23.3 degrees; k) Form XII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 5.6, 11.7, 13.8, 14.5, 16.9, 17.7, 18.7, 23.5, 24.6, 34.3, 44.2, and 44.6 degrees; l) FormXIII havingoneormorecharacteristicXRPDpeaks, in termsof2-theta (±0.2°), selected from5.7, 8.6, 9.8, 11.8, 12.6, 13.4, 14.1, 14.8, 16.6, and 19.1 degrees; m) Form XIV having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 4.0, 11.2, 11.9, 14.1, 14.8, and 15.9 degrees; or n) Form XV having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 7.4, 9.6, 12.4, 13.4, 15.5, 16.9, 17.7, 19.0, 19.5, 20.6, and 22.5 degrees. 6. The solid form for use according to any one of claims 1 and 4‑5, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for useaccording to anyoneof claims2and4‑5, or the combination for useaccording toanyoneof claims3‑5, wherein the dose of ruxolitinib, or a pharmaceutically acceptable salt thereof, is: (a) about 5 mg / day to about 60 mg / day; or (b) about 2.5 mg BID to about 30 mg BID. 7. The solid form for use according to any one of claims 1 and 4‑6, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for useaccording to anyoneof claims2and4‑6, or the combination for useaccording toanyoneof claims3‑6, wherein the dose of 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfo- nyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically acceptable salt thereof, is: (a) about 2 mg / day to about 20 mg / day; (b) about 2 mg / day to about 18 mg / day; (c) about 2 mg / day to about 12 mg / day; (d) about 4 mg / day to about 8 mg / day; (e) about 2, about 4, about 6, about 8, about 10, about 12, about 14, about 16, about 18, or about 20 mg / day on a free base basis; (f) about 2 mg / day on a free base basis; (g) about 4 mg / day on a free base basis; (h) about 6 mg / day on a free base basis; (i) about 8 mg / day on a free base basis; (j) about 10 mg / day on a free base basis; or (k) about 12 mg / day on a free base basis. 8. The solid form for use according to any one of claims 1 and 4‑7, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for useaccording to anyoneof claims2and4‑7, or the combination for useaccording toanyoneof claims3‑7, wherein the myeloproliferative neoplasm is: a) polycythemia vera (PV); b) essential thrombocythemia (ET); c) primary myelofibrosis; 21 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 d) chronic myelogenous leukemia (CML); e) chronic myelomonocytic leukemia (CMML); f) hypereosinophilic syndrome (HES); g) systemic mast cell disease (SMCD); h) chronic neutrophilic leukemia (CNL); or i) chronic eosinophilic leukemia. 9. The solid form for use according to any one of claims 1 and 4‑7, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for useaccording to anyoneof claims2and4‑7, or the combination for useaccording toanyoneof claims3‑7, wherein: (a) the myeloproliferative neoplasm is polycythemia vera (PV); (b) the myeloproliferative neoplasm is essential thrombocythemia (ET); or (c) the myeloproliferative neoplasm is primary myelofibrosis. 10. The solid form for use according to any one of claims 1 and 4‑9, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for useaccording to anyoneof claims2and4‑9, or the combination for useaccording toanyoneof claims3‑9, wherein ruxolitinib, or pharmaceutically acceptable salt thereof, and 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7-dihy- dro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically accepta- ble salt thereof, are administered orally. 11. The solid form for use according to any one of claims 1 and 4‑10, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4‑10, or the combination for use according to any one of claims 3‑10, wherein ruxolitinib, or pharmaceutically acceptable salt thereof, and 2,2,4-trimethyl‑8‑(6-methyl‑7-oxo‑6,7- dihydro‑1H-pyrrolo[2,3-c]pyridin‑4-yl)‑6‑(methylsulfonyl)‑2H‑1,4-benzoxazin‑3(4H)‑one, or a pharmaceutically ac- ceptable salt thereof, are administered simultaneously or sequentially. 22 EP 4 691 563 A2 5 10 15 20 25 30 35 40 45 50 55 23 EP 4 691 563 A2 24 EP 4 691 563 A2 25 EP 4 691 563 A2 26 EP 4 691 563 A2 27 EP 4 691 563 A2 28 EP 4 691 563 A2 29 EP 4 691 563 A2 30 EP 4 691 563 A2 REFERENCES CITED IN THE DESCRIPTION This list of references cited by the applicant is for the reader’s convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard. Patent documents cited in the description • US 63034214

[0001] • US 7598257 B

[0018] • US 20080312259 A

[0018] • US 9540368 B

[0020] • US 10189832 B

[0020] • US 10626114 B

[0034]

[0035] • WO 2002000196 A

[0092] Non-patent literature cited in the description • SHI, J. ; VAKOC, C.R. The Mechanisms Behind the Therapeutic Activity of BET Bromodomain Inhibition. Molecular Cell, 2014, vol. 54, 728-736

[0003] • FILIPPAKOPOULOS, P. et al. Selective Inhibition of BET Bromodomains.. Nature, 2010, vol. 468, 1067- 1073

[0004] • DELMORE, J.E. et al. BET Bromodomain Inhibition as a Therapeutic Strategy to Target c-MYC. Cell, 2011, vol. 146, 904-917

[0005] • LOVEN, J. et al. Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers. Cell, 2013, vol. 153, 320-334

[0005] • KLEPPE M ; KOCHE R ; ZOU L et al. Dual targeting of oncogenic activation and inflammatory signaling increases therapeutic efficacy in myeloproliferative neoplasms. Cancer Cell, 2018, vol. 33, 29-43

[0019] • Remington’s Pharmaceutical Sciences. Mack Pub- lishing Company, 1985, 1418

[0062] • BERGE et al. J. Pharm. Sci., 1977, vol. 66 (1), 1-19

[0062] • STAHL et al. Handbook of Pharmaceutical Salts: Properties, Selection, and Use. Wiley, 2002

[0062] • CHOU.DrugCombinationStudiesandTheirSynergy Quantification Using the Chou-Talalay Method. Can- cer Res, 15 January 2010, vol. 70 (2)

[0129] 摘要 本申請涉及使用 JAK1 / JAK2 抑制劑魯索利替尼與 BET 蛋白抑制劑 2,2,4-三甲基-8-(6-甲基-7-氧代-6,7-二氫-1H-吡咯並[2,3-c]吡啶-4-基)-6-(甲基磺醯 基)-2H-1,4-苯並噁嗪-3(4H)-酮組合進行的骨髓增生性贅生物的治療，其中所述組合具有 出乎意料的協同作用。

Claims

1. A solid form of a compound which is 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, or a pharmaceutically acceptable salt thereof, for use in a method of treating a myeloproliferative neoplasm in a patient in need thereof, the method comprising administering to said patient the compound, or a pharmaceutically acceptable salt thereof; and ruxolitinib, or a pharmaceutically acceptable salt thereof; wherein the solid form is Form III, Form IV, Form V, Form Va, Form VI, Form VII, Form VIII, Form IX, Form X, Form XI, Form XII, Form XIII, Form XIV, or Form XV.

2. Ruxolitinib, or a pharmaceutically acceptable salt thereof, for use in a method of treating a myeloproliferative neoplasm in a patient in need thereof, the method comprising administering to said patient ruxolitinib, or a pharmaceutically acceptable salt thereof; and a solid form of a compound which is 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, or a pharmaceutically acceptable salt thereof; wherein the solid form is Form III, Form IV, Form V, Form Va, Form VI, Form VII, Form VIII, Form IX, Form X, Form XI, Form XII, Form XIII, Form XIV, or Form XV.

3. A combination of a solid form of a compound which is 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof, for use in a method of treating a myeloproliferative neoplasm in a patient in need thereof, the method comprising administering to said patient the compound, or a pharmaceutically acceptable salt thereof, and ruxolitinib, or a pharmaceutically acceptable salt thereof; wherein the solid form is Form III, Form IV, Form V, Form Va, Form VI, Form VII, Form VIII, Form IX, Form X, Form XI, Form XII, Form XIII, Form XIV, or Form XV.

4. The solid form for use according to claim 1, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to claim 2, or the combination for use according to claim 3, wherein the ruxolitinib, or a pharmaceutically acceptable salt thereof, is ruxolitinib phosphate.

5. The solid form for use according to claim 1 or 4, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to claim 2 or 4, or the combination for use according to claim 3 or 4, wherein the solid form is: a) Form III having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 7.8, 12.4, 13.1, 15.2, 15.5, 16.9, 17.5 and 20.3 degrees; b) Form IV having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 11.2, 16.3, 18.7, and 22.1 degrees; c) Form V having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.2, 8.5, 14.1, 16.3, 17.1, 18.9, 19.8, 21.8, and 22.7 degrees; d) Form Va having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.7, 16.5, 17.3, 19.9, and 21.6 degrees; e) Form VI having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.5, 9.6, 11.4, 12.1, 13.5, 14.5, 15.2, 17.1, 17.7, 18.1, 19.2, and 20.7 degrees; f) Form VII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 9.9, 12.2, 14.8, 15.7, 17.0, 17.5, and 18.8 degrees; g) Form VIII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.1, 8.5, 16.2, 16.6, 17.0, 17.5, 18.0, 18.9, 19.6, and 20.1 degrees; h) Form IX having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.6, 9.1, 11.4, 13.4, 15.2, 18.2, 22.1, 22.8, and 23.9 degrees; i) Form X having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 14.9, 15.3, 15.8, 17.0, 17.7, 18.3, and 19.7 degrees; j) Form XI having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 8.9, 12.8, 18.0 21.5, 22.6, and 23.3 degrees; k) Form XII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 5.6, 11.7, 13.8, 14.5, 16.9, 17.7, 18.7, 23.5, 24.6, 34.3, 44.2, and 44.6 degrees; l) Form XIII having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 5.7, 8.6, 9.8, 11.8, 12.6, 13.4, 14.1, 14.8, 16.6, and 19.1 degrees; m) Form XIV having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 4.0, 11.2, 11.9, 14.1, 14.8, and 15.9 degrees; or n) Form XV having one or more characteristic XRPD peaks, in terms of 2-theta (± 0.2°), selected from 7.4, 9.6, 12.4, 13.4, 15.5, 16.9, 17.7, 19.0, 19.5, 20.6, and 22.5 degrees.

6. The solid form for use according to any one of claims 1 and 4-5, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4-5, or the combination for use according to any one of claims 3-5, wherein the dose of ruxolitinib, or a pharmaceutically acceptable salt thereof, is: (a) about 5 mg / day to about 60 mg / day; or (b) about 2.5 mg BID to about 30 mg BID.

7. The solid form for use according to any one of claims 1 and 4-6, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4-6, or the combination for use according to any one of claims 3-6, wherein the dose of 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, or a pharmaceutically acceptable salt thereof, is: (a) about 2 mg / day to about 20 mg / day; (b) about 2 mg / day to about 18 mg / day; (c) about 2 mg / day to about 12 mg / day; (d) about 4 mg / day to about 8 mg / day; (e) about 2, about 4, about 6, about 8, about 10, about 12, about 14, about 16, about 18, or about 20 mg / day on a free base basis; (f) about 2 mg / day on a free base basis; (g) about 4 mg / day on a free base basis; (h) about 6 mg / day on a free base basis; (i) about 8 mg / day on a free base basis; (j) about 10 mg / day on a free base basis; or (k) about 12 mg / day on a free base basis.

8. The solid form for use according to any one of claims 1 and 4-7, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4-7, or the combination for use according to any one of claims 3-7, wherein the myeloproliferative neoplasm is: a) polycythemia vera (PV); b) essential thrombocythemia (ET); c) primary myelofibrosis; d) chronic myelogenous leukemia (CML); e) chronic myelomonocytic leukemia (CMML); f) hypereosinophilic syndrome (HES); g) systemic mast cell disease (SMCD); h) chronic neutrophilic leukemia (CNL); or i) chronic eosinophilic leukemia.

9. The solid form for use according to any one of claims 1 and 4-7, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4-7, or the combination for use according to any one of claims 3-7, wherein: (a) the myeloproliferative neoplasm is polycythemia vera (PV); (b) the myeloproliferative neoplasm is essential thrombocythemia (ET); or (c) the myeloproliferative neoplasm is primary myelofibrosis.

10. The solid form for use according to any one of claims 1 and 4-9, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4-9, or the combination for use according to any one of claims 3-9, wherein ruxolitinib, or pharmaceutically acceptable salt thereof, and 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, or a pharmaceutically acceptable salt thereof, are administered orally.

11. The solid form for use according to any one of claims 1 and 4-10, or ruxolitinib, or a pharmaceutically acceptable salt thereof, for use according to any one of claims 2 and 4-10, or the combination for use according to any one of claims 3-10, wherein ruxolitinib, or pharmaceutically acceptable salt thereof, and 2,2,4-trimethyl-8-(6-methyl-7-oxo-6,7-dihydro-1H-pyrrolo[2,3-c]pyridin-4-yl)-6-(methylsulfonyl)-2H-1,4-benzoxazin-3(4H)-one, or a pharmaceutically acceptable salt thereof, are administered simultaneously or sequentially.

Images