KIT FOR RAPID DNA EXTRACTION IN RAW MEAT

Abstract

This invention relates to a composition of extraction kit and rapid extraction method of raw meat DNA for field application, more specifically a composition of extraction kit and rapid extraction method of raw meat DNA for field application which has the advantage of being fast and easy so that it can be applied in the field. A kit with the following composition: Lysis buffer: 0.1M Tris, 2.7mM EDTA, 0.2M NaCl, SDS 0.1% or SDS 0.05%. pH is adjusted to 8 by adding HCl. Washing Buffer: 150mM NaCl. The stages of the extraction process are lysis (by vortexing for 2 minutes at high speed), washing and elution. As an intermediary for capturing and purifying DNA using cellulose made from towel tissue that has been inserted in 200µl tips.