MULTIPLE-STEAM DISTILLATION METHOD OF ZODIA LEAVES (EUODIA SUAVEOLENS SCHEFF) TO PRODUCE ESSENTIAL OIL AS A NATURAL ANTIBACTERIAL

Abstract

This invention relates to a multistage distillation method of zodia leaves (Euodia suaveolens) carried out with the following steps: washing and cleaning fresh zodia leaves (Euodia suaveolens) from dirt and dust, inserting zodia leaves (Euodia suaveolens) into a distillation apparatus, carrying out multistage distillation two (2) times to produce 100% pure essential oil. The first stage of distillation is carried out by inserting zodia leaves into a distillator container, then filled with 5 liters of water and heated to a temperature of 100oC until all the water has evaporated. The second stage of the distillate obtained is put into a distillator and carried out a second stage distillation at a temperature of 80oC until pure essential oil is obtained.The multistage distillation method of zodia leaves (Euodia suaveolens) has inhibition against pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis which is categorized as good to very good and has a broad spectrum as an antibacterial. The main objective of this invention is to provide a multistage distillation method for zodia leaves to produce essential oils as natural antibacterials.

Description

METHOD OF STAGED STEAM DISTILLATION OF ZODIA LEAVES (EUODIA SUAVEOLENS) SCHEFF) PRODUCER OF ESSENTIAL OILS AS NATURAL ANTIBACTERIALS Invention Engineering Field This invention relates to a method for steam distillation of zodia leaves. (Euodia suaveolens) especially fractional distillation as a producer essential oils for natural antibacterial. Background of the Invention Zodiac plants have been widely used as mosquito repellent plants, especially the Aedes aegypti mosquito. Aedes aegypti mosquito attacks causes a disease that is very dangerous for humans, namely fever dengue fever. Zodia leaf extract is known to contain Several compounds have mosquito repellent properties, including evodiamine, linalool, and rutaecarpin (Research Institute for Spice Crops and Medicine / Balitro, 2007). Maryuni in 2008 revealed that zodia leaf extract has compounds that act as antibacterials, including evodone, limonene, and linalool. Various types of bacteria that can be inhibited by compounds Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis. The results of the study showed Zodia leaf extract has potential benefits for use as antibacterial and can be developed into herbal medicine. Previous inventions proposed by Marina Humbert and Lavoinne-Hanneguelle (US 8,481,088 B2) namely the manufacture of extracts Zodia leaves, formula composition, and its use as a spray mosquito repellent. A similar invention is "Pandan Essential Oil Extraction" fragrant (Pandanus amaryllifolius Roxb) With Distillation Method Multi-level” with APPLICATION NUMBER P00202106380. However, as far as search, no patent has been found for the use of the distillation method Zodia leaf tier as a producer of essential oils for natural antibacterial. To overcome the weaknesses of existing patents it was revealed that the application of the multistage distillation method of zodia leaves it turns out that it can produce pure essential oils so that it can used as a natural antibacterial. This invention uses traditional medicinal plants in the form of leaves zodia (Euodia suaveolens) which is a plant native to Indonesia. Essential oil content from the distillation of zodia leaves (Fuodia suaveolens) namely evodone, limonene, and linalool (Balitro, 2007). Compounds These chemical compounds can be used as antibacterial products Natural antibacterials generally do not cause resistance. and relatively cheap / economical compared to the use of antibiotics synthetic. Brief Description of the Invention The present invention relates to a method of fractional distillation of leaves. zodia (Euodia suaveolens) is carried out with the following steps: Wash and clean fresh zodia leaves (Fuodia suaveolens) from dirt and dust, inserting zodia leaves (Euodia suaveolens) put into the distillation apparatus, carry out distillation staged two (2) times distillation to produce 1008 pure essential oil. Multistage distillation method of zodia leaves (Euodia suaveolens), has inhibitory effects against pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis. The inhibition results are in the good to very good category. good and has a broad spectrum as an antibacterial. The main object of the present invention is to provide a method of distillation tiered for zodia leaves (EFuodia suaveolens) to produce essential oils as natural antibacterials. Complete Description of the Invention As stated in the background of the invention that the multistage distillation method of zodia leaves (Fuodia suaveolens) can produce essential oils which are useful as antibacterials natural. These results can be applied as an alternative method to address health problems, particularly in the field of antibacterials. Various forms of antibacterial medication are available on the market today. These include synthetic antibiotics. These medicinal ingredients generally expensive and often cause side effects humans and increasing cases of resistance of pathogenic bacteria to antibiotics. Currently, the use of natural medicinal ingredients is becoming more common recommended because its properties are not inferior to chemical substances, even more powerful and more economical. Fractional distillation method of zodia leaves (Fuodia suaveolens) done as follows: fresh zodia (Evodia suaveolens) leaves washed, cleaned of dirt and dust, then put into in the distillation apparatus. The fractional distillation method is carried out as many times as two times of distillation and the result is pure essential oil. The first stage of distillation is carried out by inserting zodia leaves into in a distiller container, then fill it with 5 liters of water and heated to a temperature of 100”C until all the water in The distillate has evaporated completely. The second stage of the distillate obtained put into the distillator and the second stage of distillation is carried out at a temperature of 809C until pure essential oil is obtained. Next, the results of the distillation of zodia leaves (Fuodia suaveolens) were tested. to inhibit the growth of several pathogenic bacteria, namely Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis with the agar diffusion method and then incubated for 24 hours at temperature 37 SC. Average value of inhibitory power of essential oils from distillation multilevel leaves of zodia (Euodia suaveolens) on growth test bacteria Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis shows figures that fall into the category good and very good. The essential oil content of zodia leaves is between other evodone, limonene, and linalool (Research Center for Spice Crops and Medicine / Balitro, 2007). Maryuni 2008: Sidharta and Atmodjo In 2019, it was stated that the results of the multistage distillation of zodia leaves had compounds that also have antibacterial properties, namely evodone, limonene, and linalool. The method of double-distillation filtration applied to zodia leaves can increase the content of these compounds. Increasing the levels of these compounds These compounds will result in increased antibacterial power also increased. The inhibitory power of zodia leaf essential oil against all test bacteria caused by compounds that distilled water is able to penetrate the bacterial cell walls, inhibiting formation of cell walls, inhibiting bacterial metabolic processes, and disrupt the cell division process of the test bacteria. It is suspected that these compounds such as evodone, limonene, and linalool are chemical compounds that Most of these compounds are found in zodia leaves. These compounds able to inhibit well to very well the growth of all test bacteria. The data analysis that has been carried out shows that growth test bacteria such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis can be inhibited by essential oils produced fractional distillation of zodia leaves. Essential oil compounds from this method fractional distillation with two distillations can inhibit all test bacteria for 24 hours at 37°C. These results are more preferred because it effectively inhibits the growth of all test bacteria. are pathogenic to humans. It is hoped that these results can further developed into natural medicinal ingredients derived from native plants from Indonesia. Table of inhibition results (inhibition zone diameter, cm) of essential oils against six (6) pathogenic bacteria BsECc Treatment. PaSe St.a. St. e. Oil 3.25t0.19 2.0340.22 1.5040.49 3.0740.07 3.184t0.10 2.4040.27 essential oils Control - 3.1040.10 3.4340.08 3.2540.08 3.2040.11 3.3840.12 3.1840.24 Control - 0 0 0 0 0 0 Information: B.sS.: Bacillus subtilis EB.c.:1 Escherichia coli Pa: Pseudomonas aeruginosa Se: Salmonella enteritidis Staphylococcus aureus St.e.: Staphylococcus epidermidis Control #: kanamycin antibiotic Control -: distilled water

Claims

1. A method of fractional distillation of zodia leaves (Fuodia suaveolens) done with the following steps: a wash and clean fresh zodia leaves (Fuodia suaveolens). from dirt and dust: b insert zodia leaves (Fuodia suaveolens) into distillation apparatus: c. carry out distillation in stages two (2) times distillation to produce pure essential oil 1008, d the first stage of distillation is carried out by inserting zodia leaves into the distiller container, then fill it with 5 liters of water and heated to a temperature of 1009C until all the water is used up evaporate: e second stage the distillate obtained is put into distillator and the second stage of distillation is carried out at a temperature of 80C until pure essential oil is obtained: f fractional distillation method of zodia leaves (Fuodia suaveolens) has inhibition against pathogenic bacteria such as Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Staphylococcus aureus, and Staphylococcus epidermidis which is included in the good to very good category and has a broad spectrum as an antibacterial.