COMPOSITION OF ORGANIC BIOLOGICAL FERTILIZER FOR FORESTRY SEEDLINGS BASED ON A BIOCONSORTIUM OF SUPERIOR MICROBES

Abstract

This invention relates to the composition of organic biofertilizer for forestry seedling plants based on superior microbes. The proposal of this invention is to determine the composition and method of making liquid organic biofertilizer based on superior microbial bioconsortium isolated from the rhizosphere of forestry plants, namely Hopea celebica, which is a typical plant that grows in karst areas. The uniqueness of karst formations and ecosystems allows the isolation of unique microbes that are able to adapt to extreme environmental conditions and are able to support the growth of plants that live in extreme conditions. The isolates used in this invention are Bacillus sp. JMDM 3, Bacillus velezensis JMDT 2.1, Bacillus velezensis JMDT 2.2, Burkholderia gladioli JMBT 1. The materials needed in the composition of the mother plant isolate growth media are ground corn, granulated sugar, powdered agar, fresh coconut water, fish bone waste and pure water.This invention is expected to reduce the use of chemical fertilizers and provide an alternative organic fertilizer specifically for forestry plants that is easy to make and environmentally friendly, as well as improving plant growth and health.

Description

FORMULA AND METHODS OF ORGANIC BIOLOGICAL FERTILIZER FOR SEEDLING PLANTS BIOCONSORTIUM-BASED FORESTRY OF SUPERIOR MICROBES Invention Engineering Field The present invention relates to a formula and method for manufacturing organic biofertilizer for forestry-based seedlings superior microbes. Background of the Invention Forestry plants are one of the commodities that high value both from an economic perspective and for the benefit of conservation. Currently, many plant seed nurseries are being carried out forests to overcome the scarcity of several types of forest plants, both for industrial purposes and for replanting with the aim of land conservation and rehabilitation. Very It's a shame that until now it's very rare to find fertilizer. organic biological products that are specially made to be able to applied to seedlings of forestry plant species. Nursery forestry plants still use a lot of common chemical fertilizers sold on the market. The use of chemical fertilizers is increasingly unsustainable uncontrolled, causing the soil to become saturated and leaving residues in the environment. The purpose of this invention is to find a formula and method production of Hatari organic fertilizer based on microbial bioconsortium superior isolates from the rhizosphere of forestry plants, namely Hopea celebica, which is a typical plant that grows in the area karst. The uniqueness of karst formations and ecosystems allows isolation of unique microbes that are able to adapt to conditions extreme environment and able to support plant growth who live in extreme conditions. Literature study related to this invention found that there is no patents specifically granted for organic fertilizer products forestry plant biology. However, it was found that the application for fertilizer General biological organic (patent number P00201911926) formulated with chicken manure 898, 0.058 (v / b) solvent microbes phosphate Pseudomonas trivialis, P. Putida, P. fluorescens and Aspergillus spp. and PGPR Pseudomonas fluorescens P60 and Trichoderma spp with a density of 107-9 CFU / ml, 68 humic acids fulvic acid of potato plant extract and 5g of root extract corn plants with 108 acetonitrile, Likewise, the invention This is different from the patent for Organic Biofertilizer for Rice (patent number P00201904632) with a composition of three types of active microbes namely Agrobaccoriiwi sp A8 (air N2 fixer and producer) growth hormone), Bacillus cereus TSB2 (phosphate solubilizer), and KHupenicillium sp. FG2 with powdered zeolite carrier. Meanwhile, a search for scientific journals found the influence of Combination of Endomycorrhizal Biofertilizer, Trichoderma spp., and Fertilizer Compost on the Growth of Sengon Seedlings (Paraserianthes falcataria (L.) Nielsen) by Krisdayani et al., 2020. CN109928787A by NANJING SUWUDAO INFORMATION TECH CO LTD, namely Natural biological fertilizer for grafted fruit trees (Natural biofertilizer for grafted fruit trees) which uses The main ingredient is dried animal waste. Another patent as a comparison is the manufacture of liquid fertilizer with the number KEP3960723A1 by a Spanish inventor. Core patent related to the formula and manufacture of liquid organic fertilizers made from strains Azospirillum brasilense CECT 5802 and Pantoea dispersa CECT 5801. Brief Description of the Invention The present invention relates to a formula and method for manufacturing organic biofertilizer for forestry-based seedlings superior microbes with local materials that are easy to obtain, easy made and environmentally friendly. This invention was carried out in three stages, including: 1) The stages of production of biomass inoculant for organic biological fertilizer are: isolate Bacillus sp. JMDM 3, Bacillus velezensis JMDT 2.1, Bacillus velezensis JMDT 2.2, Burkholderia gladioli JMBT 1: 2) Stages of sterilization of growth media: 3) Stages of inoculation of isolates bioconsortium on the growth medium. In the preparation stage, provided: corn kernels 17-98, 1-1.4g granulated sugar, agar 0.02-0.058, coconut flow 2.5-38, raw fish bones 7-98 water / 8-8185. At the stage of sterilization of the growing media, each material weighed and then combined into one measuring pan medium and add water. The formula mixture is cooked until boil for at least 15 minutes then strain. The filtered formula is poured into a glass bottle. 250 ml capacity as much as 100 ml using a measuring cylinder. The formula that has been poured into the glass bottle is closed with The aluminum foil is then tied with string. Formulation media has been poured into a glass bottle and then heated at room temperature at least 100”C, for t 15 minutes after boiling, After that, the mixture is cooled until it reaches room temperature. At the stage of inoculation of bioconsortium bacterial isolates, mother culture (bioconsortium bacteria combination) as much as 0.5-18 from the volume of formulation media added to the formulation media which has been cooled and then stirred, then covered with aluminum foil and tied then shaken for 1-2 weeks for obtain liquid organic fertilizer. This media formulation can be increased according to needs and used for the multiplication of bioconsortium bacterial isolates the superior one. Short Description of Image Embodiments of the present invention can be illustrated in the drawings, as presented in: Figure 1. Flowchart of the method for making organic biological fertilizer for superior microbial-based forestry seedlings (Attachment) Figure 2. Prototype of liquid organic biofertilizer product for forestry seedling plants (attachment). Complete Description of the Invention The present invention is for the purpose of a formula and method for making organic biofertilizer for forestry-based seedlings superior microbes. The media formulation is easy to make because of these ingredients. readily available, inexpensive and environmentally friendly. Invention This is done in three stages, namely 1) Formulation preparation stage media for isolate the mother stock: 2) Media sterilization stage Formulation: 3) Stage of inoculation of the parent stock into the formulation media. A. Preparation stage of media formulation for culture media parent. Materials required in the formulation of isolate growth media The main bioconsortium is corn, granulated sugar, agar-agar, coconut water, fish bones and water. The tools used include others: trays, used jam jars, aluminum foil, rubber bands, stove, steamer, water volume measuring tool, spoon / stirring stick, 708 alcohol, candle, tissue and scales. Comparison of the amount of ingredients and preparation procedures for making media are as follows: 1. Prepare the ingredients in the form of corn kernels, granulated sugar, agar- agar, coconut water, fish bones and water, 2. Weigh and mix all the media formulation ingredients with corn kernel ratio 1-98, granulated sugar 1-1.48, agar-agar 0.02-0.058, coconut water 2.5-38, raw fish bones 1-98 and water / 8-8185. 3. Cook the mixture that has been stirred evenly until it boils and then filter it twice. 4. Put the filtered formula into a glass bottle. 5. Cover the container using plastic or aluminum foil. (adjusted to availability) and tied with rope B. Stages of sterilization of formulation media Media sterilization aims to reduce the presence of various contaminating microorganisms from the formulation media. The process Sterilization is carried out as follows 1. Fill a steamer pan with water then heat until boiling Steaming the media in a pan at a temperature of 100"c, for 15 minutes after boiling,: 2. Remove the media from the steamer pot and cool until room temperature: 3. Inoculate the media with 0.5-18 parent seeds of the weight of the inoculation media. C. The inoculation stage of the parent stem Formulation of a mixture of milled corn kernels, granulated sugar, gelatin, coconut water, fish bones and water are used as carrier media for the growth of the parent anthers up to microorganisms still thrive and are easy to apply. Inoculation of parent seeds is done on the already existing place cleaned with alcohol 708 in semi-sterile conditions. Process inoculation 1. Clean the place to be used by spraying it use with alcohol 708, 2. Light the candle and place it on a place with position as needed 3. Open the container cover and inoculate the mother stock: 4. Prepare the mother stock with an age of 24 hours on NB media. (Nutrient Broth) 5. Add 24-hour-old mother stock to the media. The sterilized formulation is then stirred. Comparison The main source is 0.5-18 from the existing formula sterilized 6. Cover the container with aluminum foil and tie it then shake it for 2 weeks to get organic fertilizer liquid life

Claims

1. A formula of organic biological fertilizer for seedling plants superior microbe-based forestry consists of: Corn 1-98 Granulated sugar 1-1.4$ Agar-agar 0.02-0.058 Coconut water 2.5-38 fish bones 7-98 water 3000 mL 79-8158 2. A process of making organic biological fertilizer steps for superior microbial-based forestry seedlings: a. Prepare the ingredients in the form of corn kernels, granulated sugar, agar- agar, coconut water, fish bones and water, . Weigh and mix all the media formulation ingredients with corn kernel ratio 7-98, granulated sugar 1-1.4$8, agar- agar 0.02-0.058, coconut water 2.5-38, raw fish bones 7-98 and water 178-818. . Cook the mixture that has been stirred evenly until it boils and then filter it twice. . Put the filtered formula into a glass bottle. . Cover the container using plastic or aluminum foil (adjusted to availability) and tied with rope . Heat the formula mixture in the glass bottle with a stove at a temperature of at least 100”C, for 15 minutes after boiling, . Cool the mixture that has boiled until it reaches Room temperature. . Inoculate the 24-hour-old mother stock into the media the sterilized formulation is then stirred. The ratio of the parent stock is 0.5-18 from the formula that has been sterilized . cover the media that has been inoculated with the mother plant with aluminum foil and tied then shaken for 1-2 weeks to obtain liquid organic fertilizer and ready-to-use POH applied.