Reagent exchange in an instrument

IL279438BActive Publication Date: 2026-08-01ILLUMINA INC
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Patent Information

Application Number
IL279438
Authority / Receiving Office
IL · IL
Patent Type
Patents
Current Assignee / Owner
Priority Date
2019-06-19
Filing Date
2020-06-16
Publication Date
2026-08-01
Estimated Expiration
2040-06-16

AI Technical Summary

Technical Problem

Existing methods for determining the order of nucleotides in polynucleotides face challenges with insufficient reagent exchange, leading to cross-contamination and pre-phasing, which reduce accuracy and limit the length of complementary strands that can be built.

Method used

The method involves a reagent management system that flows a buffer reagent through a flow cell in multiple cycles of consecutive forward and reverse directions, with a higher total sweep volume compared to fluidic consumption, enhancing reagent exchange and reducing pre-phasing, allowing for longer complementary strands to be built.

Benefits of technology

This approach significantly improves reagent exchange, reducing pre-phasing and increasing the length of complementary strands that can be accurately determined, enhancing the accuracy and efficiency of nucleotide sequencing.

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Abstract

A method includes flowing an incorporation reagent through a reagent management system and a flow cell of an instrument. The flow cell having a first polynucleotide positioned therein. The incorporation reagent adding a first base onto a sequence of bases. The sequence of bases includes a second polynucleotide complementary to the first polynucleotide. An image of an identification signal emanating from the first base is captured after the first base has been added onto the second polynucleotide. A cleavage reagent is flowed through the reagent management system and flow cell to remove a first terminator from the first base in order to enable a subsequent base in the sequence of bases to be added to the second polynucleotide. A buffer reagent is flowed through the reagent management system and flow cell in a plurality of cycles of consecutive forward and reverse flow directions.
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