A method and tool to stop the growth of cancer cells and aid the wound healing process using cold plasma technology

Abstract

The present invention is a method and device for stopping the growth of cancer cells using cold plasma. In this invention, a device for producing cold plasma suitable for medical applications is disclosed; in such a way that one or more high voltage electrodes and one or more dielectric tubes are incorporated in its structure. The inlet of the device is connected to a gas reservoir with an intermediate tube, and the gas, after being converted into plasma by an electric field applied to the device, is discharged from the device. The inventive device is designed to prevent electrical discharge during the process. The process, which stops the growth of cancer cells or heals wounds, begins by receiving an electric field with a specific voltage, frequency, waveform, and duty cycle; these parameters vary depending on the location of the cancer cells and the severity of the cancer, as well as the type and severity of the wound. This electric field is then applied to the inventive device to convert the gas inside it into cold plasma. The cold plasma produced is released from a series of devices that, when applied to the cancerous or wound area, lead to the death of cancer cells or wound healing; without causing damage to healthy cells and their growth cycle or other biological tissues. The present achievement is designed in such a way that it can replace current high-risk treatment methods.

Description

Description of the invention Title of the invention (as stated in the declaration) A method and tool to stop the growth of cancer cells and aid in the wound healing process using cold plasma technology Title: Method and apparatus for stopping cancer cells growth and for helping wound healing process using cold plasma technology Technical background of the relevant invention The present invention generally relates to the field of medicine and, more specifically, to the field of plasma therapy, and more specifically, to stopping the growth of cancer cells and aiding the wound healing process using cold plasma technology. Technical problem and stating the objectives of the invention Due to modern life, lifestyle changes and reduced physical activity, diseases such as diabetes are increasing rapidly. Caring for these patients and the side effects of long-term exposure to this disease are one of the problems of today's society due to its high prevalence. One of these cases is the formation of wounds in the lower limbs of diabetic patients, which are very difficult to heal due to impaired cellular metabolism and mainly lead to amputation. By finding new and appropriate solutions and using new methods such as plasma, a great change can be made in reducing costs and improving the quality of life of these people. Studies have shown that plasma has very positive and dramatic effects in the treatment and healing of chronic and acute wounds, and plasma can be used as a new treatment method in wound healing (resulting from different factors). The number of cancer cases is increasing in both developed and developing countries, and cancer remains a major health problem that has placed a heavy burden on families and society in recent years.Despite many scientific advances, cancer recurrence and resistance to treatment are among the problems of cancer patients. Therefore, it is necessary to introduce new therapeutic strategies for cancer treatment and wound healing. One of these modern methods is the use of plasma in medicine. Due to the increased tendency of cancer cells to absorb free radicals to continue the process of cellular metabolism and as a result of the growth and proliferation of these cells, plasma therapy, by producing reactive oxygen and nitrogen species and other free radicals, accelerates this process, causing the production of a large amount of toxins in the cancer cell and the initiation of apoptosis (spontaneous cell death) in the cancer cell and as a result, the death of these cells. Also, the cold plasma produced in the plasma therapy process destroys microorganisms, creates a balance in cytokines, stops and coagulates bleeding, increases factors effective in angiogenesis and accelerates the wound healing process and oxygenates the cells, and creates chemical changes to absorb precursor cells, causing wound healing. It should be noted that the amount of cold plasma irradiated and the treatment time vary according to the type of cancer or wound in question. As the energy applied to the atoms of a solid increases, the thermal motion of the atoms increases, and this energy can gradually overcome their binding energy and lead to a change from the solid to the liquid state. It is obvious that by increasing the energy applied to the atoms in the liquid state, the gaseous state of the material can be achieved. Now, if the energy is increased so much that the electrons can overcome their electrostatic potential barrier and separate from the atom, the phase transition from the gas to the plasma state occurs. Plasma is a mixture of electrons, ions, neutral particles, an electromagnetic field, and ultraviolet radiation, all of which exhibit collective behavior. Plasma is electrically neutral, but due to the presence of charge carriers, it is an electrical conductor. On the other hand, due to the presence of active species, it is considered a very active chemical environment. Plasma is generally a neutral ionized gas consisting of positively charged ions, electrons, and neutral particles. The temperature of the plasma is determined by the thermal motion of electrons and heavy particles such as atoms and ions.Basically, plasma at atmospheric pressure can be divided into two classes: thermal and non-thermal. Non-equilibrium discharges at atmospheric pressure are produced in various configurations. These configurations are generally specific to industrial applications, so there are various techniques for producing the configurations. The energy of electrons in non-equilibrium plasma is much higher than the energy of ions and other neutral species present in the plasma. The reactions in non-equilibrium plasma do not increase the enthalpy of the gas and the materials that come into contact with them do not suffer any thermal damage and, due to its low gas temperature, are widely used for materials that are sensitive to heat. Also, collisions at atmospheric pressure lead to three-body reactions to produce oxidizable species such as ozone and excimers, whose applications have increased in various fields.In fact, the most common configurations of these sources have been plasma jets, which can be broadly classified into single-array jets for cancer treatment and multi-array jets for wound treatment. Single-array jets can be used to treat very small nodules and tumors, ranging from 1 to 10 mm, one-by-one, while multi-array jets can be used to treat larger tumors, as well as cancerous, diabetic, and other wounds. In view of the above explanations, it is necessary and necessary to develop a method and manufacture a tool to prevent the growth, proliferation and progression of cancer cells and tumors, as well as to treat and repair wounds without damaging healthy tissues. The present achievement, by presenting a method based on cold plasma technology, provides a process for treating cancer and repairing wounds. The present invention, with its unique design, prevents electrical discharge during the treatment process. However, it transfers the power necessary for cancer cell death to the cancer tissue without damaging healthy tissues. The tool provided in the present invention is portable and can be used in medical centers and hospitals for cancer treatment. A description of the state of the prior art and the history of developments related to the claimed invention. The use of cold plasma-based therapy for the treatment of cancer cells and wound healing has been investigated in various studies both domestically and internationally. For example, in 2017, a method for treating cancer stem cells using a device based on cold plasma technology was proposed and presented in an invention with application number US2017 / 0183632A1. In 2018, another invention with application number US10023858B2 introduced a method for inducing apoptosis in cancer cells using cold plasma technology. In 2021, a combined method for treating cancerous mass tumors based on cold plasma technology was presented in an invention with application number US2021 / 0196337A1. In 2018, a method based on cold plasma technology for treating skin diseases was also proposed in an invention with application number US2018 / 0318596. In 2021, a combined method with cold plasma for wound treatment was presented in a patent application number US2021 / 0030596A1. The examples mentioned and other developments in this field indicate the success of this method in the medical field, especially in cancer treatment and wound healing.However, using existing technologies for all types of cancer, or more precisely for high-risk cancers in sensitive areas of the brain, requires many considerations; because in brain areas, due to the presence of sensitive nerve fibers, treatment is very precise and risky, and the treatment method must be such that these fibers are not damaged, which is often irreparable. The present invention, regardless of the various embodiments it may have, is a method and tool for stopping the growth of cancer cells for the purpose of treating cancer and also for wound healing using cold plasma technology. The present achievement, due to its unique design, is considered a suitable tool for the death of cancer cells without damaging healthy cells and also for wound healing without biological damage. The structure designed in this invention is such that it prevents electrical discharge and causing irreparable damage to the patient. The present achievement, due to the simplicity of providing the necessary facilities and their portability, can be used in all medical centers and hospitals to effectively inhibit the growth of cancer cells and heal various wounds, so it can be an effective alternative to current harmful methods in the treatment of cancer, even in its severe form, and in wound healing. Providing a solution to an existing technical problem along with an accurate, sufficient, and integrated description of the invention As mentioned, the present invention provides a method and tool for stopping the growth of cancer cells and healing wounds such as cancer and diabetic wounds using cold plasma technology. The design of the present invention is such that it is effective for all types of cancers, even those located in sensitive areas of the brain, and without harming healthy cells; it stops the growth of cancer cells and accelerates the wound healing process. This design is also such that any biological damage is prevented from entering the patient's body during wound healing. Except for the concepts specifically defined in the context of the present invention, all technical and scientific terms used are to be understood as understood by those knowledgeable or skilled in the field (the fields covered by the present invention). An attempt has been made here to mention the most preferred materials and tools for describing the method, although many similar materials and tools can be used to describe the present invention and / or to carry out the experiments and processes of the invention. The following is a description of one or more embodiments of the present invention, based on the form provided in the "Technical Drawing" file. It should be noted that these embodiments merely indicate one or more representatives of the present invention, and therefore the present invention is not specifically limited to any of these cases. In one or more embodiments of the present invention, a device designed to stop the growth of cancer cells or to heal wounds includes a protective body, a tool set, a dielectric tube, a high-voltage electrode, a high-voltage generating power supply, and a gas capsule; wherein the protective body includes a hole in the center, along and coaxial with the protective body, which creates a void from the beginning to the end of the protective body; wherein this overall hole at the beginning of the protective body is the gas inlet and the end of the protective body is connected to the tool set. In one embodiment of the present invention, the protective body is screwed to the tool set. In one embodiment of the present invention, the initial portion of the dielectric tube is located at the end of the overall hole provided in the protective body, and the end portion of the dielectric tube is located in the tool set. The high-voltage electrode is in the form of a cylinder that covers the surface of the end portion of the dielectric tube. In various embodiments of the present invention, the gas tank is connected to the beginning of the protective body with an intermediate pipe, and through this intermediate pipe, the gas is directed from the tank to a cavity located inside the protective body.In one or more embodiments of the present invention, the electric field generating source is connected to the high voltage electrode and thereby applies an electric field to the tool. In this way, the gas introduced into the overall cavity embedded in the protective body is converted into cold plasma under the influence of the electric field of the high voltage electrode and is discharged from the end of the protective body connected to the tool series. In various embodiments of the present invention, the cold plasma generated has a temperature close to room temperature and is not possible to discharge due to the embedded dielectric tube. In a general aspect of the present invention, the method and device provided can be used to stop the growth of cancer cells including skin cancer, breast cancer, prostate cancer, brain tumors and other cancers. In various embodiments of the present invention, depending on the type and severity of the cancer, the parameters of the electric field generating source, which can be a direct or alternating power supply, are adjusted to achieve the desired power and applied to the high voltage electrodes; in such a way that these parameters include the waveform, frequency, amplitude and duty cycle of the signal generated by the power supply. In one embodiment of the present invention, an alternating power supply that generates a sine, square, triangular, ramp, step, and / or a combination of these waveforms is used and the amplitude of the generated waveform is in the range of [0-15] kilovolts and its frequency is in the range of [0-20] kilohertz; In various embodiments of the present invention, the parameters of the power supply are adjusted to produce power appropriate to the desired application, which is ultimately 1 to 2 watts.In the general aspect of the present achievement, controlling the parameters of the electric field generation source in accordance with the nature of cancer cells, along with the structure of the innovative tool for producing cold plasma, stops the growth of cancer cells without damaging healthy tissues. In one or more embodiments of the present invention, the gas-containing reservoir may comprise a noble gas, oxygen, and / or a mixture thereof. In various embodiments of the present invention, the high voltage electrode may be made of copper, silver, steel, and / or any similar electrically conductive material. In one or more embodiments of the present invention, the dielectric tube may be made of quartz, ceramic, glass, and / or any other similar material. In one embodiment of the present invention, the protective body may be made of polyethylene, Teflon, and / or any other similar material. The following are some examples in accordance with one or more embodiments of the present invention in full. It should be noted that these examples are merely provided for a better understanding of the technology provided and the present invention can be prepared without such details. As previously stated, these examples are described only as one or more examples of the present invention and do not limit the scope of protection of the present invention in any way, and other methods are also within the scope of the present invention. Figure 1-A is a schematic diagram of a cold plasma generation device (100) according to one or more embodiments. The device (100) is comprised of a protective body (101) and a series of devices (107); such that cylindrical cavities (106) are provided inside the protective body (101) and coaxially therewith, and gas enters the cylindrical cavity (106) provided in the protective body (101) from the inlet side and, after being transformed into cold plasma under the influence of an electric field applied to the high voltage electrode (103), exits the series of devices. The device (100) also includes a dielectric tube (102) that prevents electrical discharge when applying plasma to the cancer cell, and the initial part of this dielectric tube (102) is placed at the end side of the cylindrical cavity embedded in the protective body (101), and the end part of this dielectric tube (102) is placed in the device series (107). The high voltage electrode (103) is designed as a cylinder that covers the surface of the end part of the dielectric tube (102) and is placed in the device series (107). Figure 1-B shows a schematic of a cold plasma technology-based device (200) for applying cold plasma to a wound in accordance with one or more embodiments. In this arrangement (200), one of the outputs of an alternating current power supply is connected by a conductive wire (209) to a high voltage electrode (207) in the cold plasma generation device (200). A gas-containing reservoir is connected to the front of the protective body (206) of the cold plasma generation device (200) via an intermediate tube (208). In other embodiments, the electric field generator and the gas reservoir can be designed with appropriate dimensions within a single assembly. By adjusting the voltage, frequency, waveform and duty cycle of the alternating current power supply, a suitable electric field is applied to the high voltage electrode (207) depending on the type and severity of the wound. In this way, the gas that is directed from the gas tank through the intermediate tube (208) to the cold plasma generation tool (200) is converted into cold plasma with a temperature close to room temperature in the field created in the dielectric tube (203) and exits the tool series (201) and is ready to be applied to the desired wound area. Explanation of shapes, maps and diagrams The figures presented illustrate one or more embodiments of the present invention by way of example only. Therefore, the figures presented do not limit the scope of the present invention. Figure 1-A shows a schematic of a cold plasma generation device for cancer treatment in accordance with one or more embodiments. Figure 1-B shows a schematic of a cold plasma generation device for wound healing in accordance with one or more embodiments. A clear and precise statement of the advantages of the claimed invention over prior inventions. The present achievement is a method and tool for stopping the growth of cancer cells and healing wounds using cold plasma technology. The advantages of this product over previous knowledge include: 1. Stopping the growth of cancer cells without harming the growth of healthy cells and healing wounds without harming other biological tissues; 2. No formation of electrical discharge phenomenon when working with cold plasma, thus ensuring the absence of the possibility of irreparable damage caused by electrical discharge; 3. Suitable for tumors located in sensitive areas of the brain and wounds located in sensitive areas of the body, where conventional methods are too risky; and 4. No side effects for the patient. Description of at least one implementation method for implementing the invention The present achievement, regardless of its possible embodiments, is a method and tool for stopping the growth of cancer cells and healing wounds using cold plasma technology. In an example of the present invention, a single-array jet-based cold plasma generation device is composed of a cylindrical polyethylene protective body, such that the diameter of the protective body is 3.1 cm, and a hole with a diameter of 16 mm is provided in the center and coaxially with the protective body. A quartz dielectric tube with an inner diameter of 4 mm, a thickness of 1 mm, and a length of 5 cm is placed at the end of the overall hole provided in the protective body, and the end portion of this dielectric tube is placed in the tool series; such that a high-voltage electrode in the form of a cylinder made of copper with an inner diameter of 5 mm, a thickness of 1 mm, and a length of 3 cm is prepared to cover the surface of the end portion of the dielectric tube. An intermediate tube connects the helium gas tank to the beginning of the cylindrical hole provided in the protective body; In such a way that helium gas is directed from the gas tank through an intermediate tube into the overall cavity embedded inside the protective body.In order to use this preferred embodiment of the present invention for cancer treatment, the sinusoidal alternating power supply is set to a frequency between 13 and 20 kHz and a range of 10 to 15 kV. The control of the power supply as described above, together with the disclosed structure for the cold plasma generation device, results in the death of cancer cells, while the patient is not subjected to electric shock or other irreparable damage due to the impossibility of electric discharge. Furthermore, existing nerve fibers and other cells and tissues existing alongside the cancer cells are not damaged and continue to grow normally. In another example of the present invention, a multi-array jet-based cold plasma generation device is composed of a handpiece made of reinforced polyethylene (PETG), such that the diameter of the handpiece opening is 8 cm and 13 holes with a diameter of 6 mm are located in the handpiece opening coaxially with the embedded quartz tubes, such that high-voltage electrodes in the form of copper spring wires with a length of 1 to 3 cm are placed around the dielectric quartz tubes and cover the surface of the middle part of the dielectrics. An intermediate tube connects the helium gas tank to the beginning of the handpiece body embedded in the protective body; such that the helium gas from the gas tank is guided by the intermediate tube into the overall hole embedded in the protective body. In order to use this preferred embodiment of the present invention for wound healing, the sinusoidal alternating current power supply is set to a frequency between 15 and 20 kHz and an amplitude of 10 to 16 kV.The above-described power supply control, together with the disclosed structure for the cold plasma generation device, results in accelerated wound healing, while the patient is not subjected to electric shock or other irreparable damage due to the impossibility of electrical discharge. Furthermore, existing nerve fibers and other cells and tissues adjacent to the wound area are not damaged and continue to grow normally. Explicit mention of the industrial application of the invention The present achievement, regardless of the fact that it can have different embodiments, is related to the field of plasma therapy for cancer treatment and wound healing; in a way that prevents the growth of cancer cells by causing cell death without harming the growth of healthy cells. It also helps in wound healing by accelerating the wound healing process. The disclosed device is portable and does not require complex ancillary facilities and can be mass-produced. Therefore, the presented method and device can replace the current conventional methods in all medical centers and hospitals for cancer treatment and wound healing. Brief description of the invention The present invention is a method and tool for stopping the growth of cancer cells using cold plasma. In this invention, a tool for producing cold plasma suitable for medical applications is disclosed; in such a way that one or more high-voltage electrodes and one or more dielectric tubes are embedded in its structure. The inlet of the tool is connected to a gas reservoir with an intermediate tube, and the gas is discharged from the tool after being converted into plasma by an electric field applied to the tool. The inventive tool is such that it prevents electrical discharge during the process. The process that leads to stopping the growth of cancer cells or wound healing begins by receiving an electric field with a specific voltage, frequency, waveform, and duty cycle; in such a way that these parameters vary depending on the location of the cancer cells and the severity of the cancer progression, as well as the type and severity of the wound in question. Then, this electric field is applied to the inventive tool so that the gas inside it is converted into cold plasma.The cold plasma produced is released from the device, which, when applied to the cancerous or wound area, leads to the death of cancer cells or wound healing; without causing damage to healthy cells and their growth cycle or other biological tissues. The present achievement is designed in such a way that it can replace current high-risk treatment methods.

Claims

Claims What is claimed: Claim 1) A device for stopping the growth of cancer cells or healing wounds using cold plasma technology, comprising: - a protective body, which protective body includes a cylindrical cavity in the center and coaxial with the protective body, which creates an empty space from the beginning to the end of the protective body; in such a way that this cylindrical cavity at the beginning of the protective body is the place of gas entry or this protective body is designed in the form of a handpiece; - a series of tools or a handpiece with a hole; in such a way that this series of tools or a handpiece with a hole is connected to the end of the protective body and the cold plasma produced is discharged from the series of tools or the handpiece with a hole; - one or more dielectric tubes; In such a way that the initial part of these dielectric tubes (s) is placed at the end side of the tool set or the hollow opening of the handpiece embedded in the protective body and the final part of these dielectric tubes (s) is placed in the tool set or the hollow opening of the handpiece; In such a way that these dielectric tubes (s) prevent electrical discharge when applying cold plasma to the cancer cell or wound area; -One or more voltage electrodesAbove; where these high voltage electrodes are cylindrical in the form of copper plates or spring wires that cover the surface of the end part of the dielectric tube or the middle part of the dielectric tubes; - a source for generating an electric field; in such a way that this source for generating an electric field is connected to the high voltage electrode; and - a gas reservoir; in such a way that this gas reservoir is connected by an intermediate tube to the beginning of a cylindrical cavity embedded in the protective body or handpiece and directs the gas into the cylindrical cavity embedded in the protective body to be converted into cold plasma under the influence of the said electric field; in such a way that this cold plasma produced is discharged from the tool head or the mouth of the handpiece and applied to the cancer cells or the wound area; Claim 2) A method for stopping the growth of cancer cells or healing wounds using cold plasma according to claim 1, wherein the cold plasma generating device is composed of a protective body, a tool or handpiece opening, one or more dielectric tubes, one or more high-voltage electrodes; in such a way that the protective body includes a global cavity that creates a free space from the beginning to the end of the protective body; in such a way that this global cavity is the gas inlet at the beginning of the protective body and the end of the protective body is connected to the tool or handpiece opening; in such a way that the initial part of the dielectric tube(s) is placed at the beginning of the global cavity embedded in the protective body and the final part of the dielectric tube(s) is placed in the tool (handpiece) series; in such a way that the high-voltage electrode(s) are cylindrical and cover the surface of the end part of the dielectric tube or the surface of the middle part of the dielectric tube. The inlet of the tool is connected to a gas reservoir with an intermediate tube, and the gas exits the tool head after being converted into plasma by the electric field applied to the tool.This electric field is applied to the innovative tool to convert the gas inside it into cold plasma. The cold plasma produced is released from the tool, which, when applied to the cancerous or wound area, leads to the death of cancer cells or wound healing; without causing damage to healthy cells and their growth cycle or other biological tissues. The innovative tool is designed to prevent electrical discharge during the process.

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