Natural toothpaste with a combination of vegetable oils and propolis extract

Abstract

Gingivitis and plaque buildup are common oral problems that can lead to periodontal disease and tooth decay. To address this problem, we have created a toothpaste that contains ingredients such as; a combination of natural extracts and propolis extract, miswak extract, potassium nitrate, xylitol, low-abrasive hydrated silica, glycerin, honey, carboxymethyl cellulose, cocamidopropyl betaine, licorice extract, nano-turmeric, sodium benzoate, vitamin E, menthol, sodium hydroxide, and deionized water. The product production method is as follows: preparation of the aqueous phase, emulsification of the oily phase, addition of active ingredients, addition of abrasives and flavorings, adjustment of pH with sodium hydroxide, and finally packaging. This toothpaste is suitable for use in the oral hygiene products industry, especially for consumers with tooth sensitivity and gum problems.

Description

Description of the invention Title of the invention Natural toothpaste with a combination of vegetable oils and propolis extract Technical background of the relevant invention This product is for brushing teeth and the technical field of the product is health and hygiene. Technical problem and stating the objectives of the invention Gingivitis and plaque accumulation are common oral problems that can lead to periodontal disease and tooth decay. Tooth sensitivity, caused by receding gums or loss of tooth enamel, is also common in many people. Commercially available toothpastes often contain chemicals such as sodium lauryl sulfate (SLS), which may cause irritation in patients with tooth sensitivity. Clinical studies such as (Jayashankar et al., 2011; Azaripour et al., 2015; Michałowski et al., 2025) have shown that herbal toothpastes containing natural extracts such as Salvadora persica (miswak), Syzygium aromaticum (clove), and Sinapis alba (mustard) can effectively reduce plaque index (PI), bleeding on probing (BOP), and sulcus bleeding index (SBI). However, existing formulations may lack the optimal combination of natural oils and extracts that simultaneously target tooth sensitivity, gingivitis, and plaque. This product offers an innovative formulation that addresses these needs using natural and safe ingredients. A description of the state of the prior art and the history of developments related to the claimed invention. Toothpaste using mulberry fruit extract and hydroxyapatite with antibacterial properties and tooth enamel reconstruction, registration number 100146 Tooth whitening and brightening toothpaste formulation based on encapsulated sumac extract, registration number 106880 Astragalus herbal toothpaste with analgesic and antibacterial effects, registration number 105039 Herbal toothpaste containing berberine and saponin, registration number 105393 Of course, a variety of products have entered the global market in this field, but the toothpastes available are often: Contain irritating ingredients such as (SLS) which are not suitable for patients with tooth sensitivity. They lack an optimal combination of natural ingredients with clinically proven effectiveness to simultaneously reduce gingivitis, plaque, and tooth sensitivity. May not have sufficient stability and sensory acceptance (taste, texture), especially in formulations containing vegetable oils. Providing a solution to an existing technical problem along with an accurate, sufficient, and integrated description of the invention This product offers a natural toothpaste with the following formulation that simultaneously targets tooth sensitivity, gingivitis, and plaque buildup: Combination of natural extracts and propolis extract (1% w / w): Contains Syzygium aromaticum extract (0.25%), Commiphora myrrha extract (0.15%), and propolis extract (0.15%) for antibacterial, anti-inflammatory, soothing, and antioxidant properties. Salvadora persica miswak extract (1%): To reduce sulcus bleeding index (SBI) and adjacent plaque index (API). Potassium nitrate (5%): To reduce tooth sensitivity by blocking dentinal canals. Xylitol (8%): ​​For anti-plaque and anti-caries effects. Low abrasive hydrated silica RDA <70 (12%): For gentle plaque removal without damaging enamel or dentin. Other ingredients: Glycerin (25%), honey (0.25%), carboxymethyl cellulose (1%), cocamidopropyl betaine (5.1%), licorice extract (5.1%), nano-turmeric (1.0%), sodium benzoate (3.0%), vitamin E (1.0%), menthol (2.0%), sodium hydroxide (less than 1.0%), and deionized water (~41.6%). Using natural and safe ingredients, this formulation minimizes irritation and offers clinical efficacy based on validated studies (Jayashankar et al., 2011; Azaripour et al., 2015; Michałowski et al., 2025). Production method: Preparation of the aqueous phase: Dissolve glycerin, xylitol, honey, carboxymethyl cellulose, and sodium benzoate in deionized water (60–70°C) until a homogeneous mixture is formed. Emulsification of the oil phase: Mix the oils of S. aromaticum, A. chundra, Z. officinale, C. myrrha, S. alba, and propolis extract with cocamidopropyl betaine to form a stable emulsion. Adding active ingredients: We add Miswak extract, licorice extract, nano-turmeric, potassium nitrate, sodium tripolyphosphate, and vitamin E to the mixture. Adding abrasive and flavoring: Gradually add hydrated silica and menthol and stir until the paste is smooth. Adjusting pH with sodium hydroxide: Adjust the pH between 2.7 – 8.6. Packaging: Package the paste in airtight tubes or pumps to prevent oil degradation. Evaluation tests in vitro: Minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) for S. mutans and P. gingivalis bacteria. Inhibition and destruction of biofilm with crystal violet and CLSM Obstruction of dentinal canals with SEM Inhibition of inflammatory cytokines by (1 β-IL, α-TNF) ELISA Accelerated stability test (40°C), 6 months. ex vivo: dynamic biofilm models in the oral reactor. in vivo: Randomized, double-blind clinical trial to measure PI, BOP, SBI, and VAS (sensitivity) at 4–12 weeks. Expected results Gingivitis: BOP reduction to (6.30%) and SBI (1.17-5.14%) similar to reference studies. Plaque reduction (43.2) PI and API (3–4.3%) with antibacterial effects of oils and propolis. Tooth sensitivity: pain reduction (VAS) with potassium nitrate and low-abrasive silica. Safety: No irritation or allergic reaction, with monitoring of propolis and mustard oil. Explanation of shapes, maps and diagrams Table 1: Compositions and weight percentages Table 2: Solution concentrations for antioxidant tests using the DPPH method, biofilm formation destruction, biofilm formation inhibition and MTT assay cell viability: Given that 100 μl of the obtained solution was poured into each well. The first well was considered as the highest concentration and 100 and the other wells are in the form of a table. Table 3: Antioxidant test by DPPH method: The percentage of DPPH inhibition is expressed based on the concentrations of the tested substances, and higher numbers indicate higher antioxidant properties. Control sample absorbance (DPPH only): 0.883 Numbers are expressed as percentage of DPPH inhibition and are the average of three replicates. Table 4: Biofilm formation destruction test: The purpose of this test was to investigate the anti-biofilm properties of the compounds based on the international standard ASTM E2799-17 on Streptococcus mutans bacteria. Table 5: Biofilm formation inhibition test: The purpose of this test was to investigate the anti-biofilm properties of the compounds based on the national standard 16684 on the standard bacteria Streptococcus mutans. Table 6: Preservative efficacy assay: If the following results are obtained, the preservative in the product has the required efficacy. The number of gram-positive, gram-negative bacteria and yeasts should be reduced by at least 99.9% within seven days of the initial inoculation and should not increase beyond the normal range of standard deviations during the remainder of the test period. The number of fungi should be reduced by at least 90% within 28 days of the initial inoculation and should not increase beyond the normal range of standard deviations during the remainder of the test period. Microorganisms should grow in all untreated samples, which are tested as controls. Table 7: Concentration of solution for MIC and MBC activity assay: Given that 100 μl of the obtained solution was poured into each well. The first well was considered as the highest concentration and 100 and the other wells are in the form of a table. Table 8: MIC and MBC activity assay Table 9: 24-hour microbial count test and antibacterial claim verification: Sample units are based on CFU / ml or viable colony count per ml. The reported numbers are the average of three test replicates. Table 10: MTT assay cell viability test A clear and precise statement of the advantages of the claimed invention over prior inventions. - Multiple efficacy: Simultaneous reduction of gingivitis (SBI and API), (BOP, PI plaque) and tooth sensitivity (VAS) with natural ingredients. - Safety for sensitive patients: No use of SLS and reduced menthol concentration (2.0%) to prevent irritation of sensitive tissues. - High sensory acceptance: The combination of honey, licorice, and xylitol creates a pleasant taste and texture. - Formulation stability: Use of cocamidopropyl betaine and carboxymethyl cellulose to emulsify oils and maintain stability. - Clinical support: Based on clinical results showing significant reductions in PI (p<0.001), BOP (p<0.001), and SBI (14.5–17.1%) similar to reference studies Description of at least one implementation method for implementing the invention Its production process is possible with industry-standard equipment, and packaging in airtight tubes facilitates commercial distribution. Explicit mention of the industrial application of the invention This toothpaste is suitable for use in the oral hygiene products industry, especially for consumers with tooth sensitivity and gum problems.

Claims

Complaint What is claimed: Claim 1) The product (toothpaste) is natural and is made from natural extracts and propolis extract (1% w / w) including; Syzygium aromaticum extract (0.25%), Commiphora myrrha extract (0.15%) and propolis extract (0.15%), Salvadora persica miswak extract (1%), potassium nitrate (5%), xylitol (8%), low-abrasive hydrated silica RDA <70 (12%), glycerin (25%), honey (0.25%), carboxymethyl cellulose (1%), cocamidopropyl betaine (5.1%), licorice extract (5.1%), nano-turmeric (1.0%), sodium benzoate (3.0%), vitamin E (1.0%), menthol (2.0%), sodium hydroxide (less than 1.0%) and deionized water (~41.6%).