Additive composition for feed

Abstract

To provide an additive composition for feed and a feed composition with which it is possible to promote digestion of protein in fish, shellfish, and the like that have ingested feed containing protein.SOLUTION: An additive composition for feed is composed of (A) an anionic surfactant and (B) a nonionic surfactant, and a mass ratio [(A) / (B)] of the (A) component content and the (B) component content is 0.7-20 inclusive.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to a feed additive composition and a feed composition. [Background technology]

[0002] In recent years, in aquaculture, feeding of compound fish feed such as pellets has become the norm, replacing the traditional live feed. In order to improve the quality of fish and shellfish, efforts are underway to develop better aquaculture feed.

[0003] Patent Document 1 discloses a method for producing solid feed, which is characterized by adding an emulsion obtained by emulsifying a water-soluble polymer aqueous solution and fish oil with an emulsifier (polyoxyethylene sorbitan fatty acid ester, etc.) to a feed material. Patent Document 2 discloses a method for producing dry pellets, which is characterized by adding 0.1 to 10% by weight of a nonionic surfactant (polyoxyethylene glycerin fatty acid ester, etc.) to the fat and oil when producing dry pellets with a fat and oil content of 10 to 50% by weight. Patent Document 3 discloses a live feed composition, which is characterized by containing thiamine or thiamine salts (thiamine nitrate, etc.) coated with a coating agent consisting of fat and oil having a melting point of 50 to 80°C and lecithin, or fat and oil having a melting point of 50 to 80°C, lecithin, and polyglycerol polymerized fatty acid ester, in a live feed, in an amount of 1 / 3 to 1 times the amount of thiamine or thiamine salts (thiamine nitrate, etc.), and discloses that the feed composition can prevent thiamine deficiency in fish. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] JP 2003-235471 A [Patent Document 2] Japanese Patent Application Publication No. 3-290156 [Patent Document 3] Japanese Patent Application Publication No. 59-66844 Summary of the Invention [Problem to be solved by the invention]

[0005] One example of the development of feed for farming fish and shellfish (hereinafter referred to as fish feed) is a method for increasing the weight of individual fish and shellfish, and for this purpose, it is desirable to promote the digestion of protein in the bodies of fish and shellfish. Therefore, an object of the present invention is to provide a feed additive composition and a feed composition that can promote the digestion of protein in fish and shellfish that have ingested feed containing protein. [Means for solving the problem]

[0006] In one embodiment, the present invention provides a feed additive composition comprising an anionic surfactant (A) [hereinafter referred to as component (A)] and a nonionic surfactant (B) [hereinafter referred to as component (B)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0007] In another embodiment, the present invention provides a feed composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)] and (C) a protein [hereinafter referred to as component (C)], wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less. Effect of the Invention

[0008] The feed additive composition and feed composition of the present invention can promote protein digestion in fish and shellfish that have ingested protein-containing feed, thereby improving the quality of the fish and shellfish. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] [Feed additive composition] The feed additive composition of the present invention is a feed additive composition comprising an anionic surfactant (A) and a nonionic surfactant (B), and the mass ratio of the content of the component (A) to the content of the component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0010] In the feed additive composition of the present invention, component (A) acts on a protein to change the structure of the protein and thereby promote digestibility.

[0011] Examples of the component (A) include one or more selected from alkyl sulfates, alkylbenzenesulfonates, alkylsulfosuccinates, and cholate salts. The salt of the component (A) may be preferably an alkali metal salt such as a sodium salt or a potassium salt, or a thiamine salt.

[0012] From the viewpoint of improving digestibility, the alkyl sulfate may have an alkyl group having preferably 8 or more carbon atoms, more preferably 10 or more carbon atoms, and 18 or less carbon atoms, more preferably 16 or less carbon atoms. The alkyl sulfate may have either a straight chain or a branched chain alkyl group, and from the viewpoint of improving digestibility, the alkyl sulfate may preferably have a straight chain alkyl group.

[0013] In the present invention, alkyl sulfates having a straight-chain alkyl group having 10 or more carbon atoms and 16 or less carbon atoms are preferred. Examples of salts constituting the alkyl sulfates include alkali metal salts such as sodium and potassium, alkanolamine salts, and thiamine salts. From the viewpoint of improving digestibility, sodium salts are preferred, and from the viewpoint of improving intake, thiamine salts are preferred.

[0014] Thiamine is also known as vitamin B1, and it is known that vitamin B1 is added to feed to produce livestock with a high vitamin B1 content. However, the technology of adding an anionic surfactant such as a thiamine salt to feed to promote digestion of protein-containing feed was not known.

[0015] From the viewpoint of improving digestibility, the alkyl group of the alkylbenzenesulfonate may preferably have 8 or more carbon atoms, more preferably 10 or more carbon atoms, and preferably have 18 or less carbon atoms, more preferably have 16 or less carbon atoms. The alkyl group of the alkylbenzenesulfonate may be either linear or branched, and from the viewpoint of improving digestibility, the alkyl group is preferably linear.

[0016] In the present invention, the alkylbenzenesulfonate is preferably an alkylbenzenesulfonate having a linear alkyl group having 10 or more carbon atoms and 16 or less carbon atoms. Examples of salts constituting the alkylbenzenesulfonate include alkali metal salts such as sodium and potassium, and thiamine salts. From the viewpoint of improving digestibility, sodium salts are preferred, and from the viewpoint of improving feed intake, thiamine salts are preferred.

[0017] From the viewpoint of improving digestibility, the alkyl sulfosuccinate may have an alkyl group having preferably 6 or more carbon atoms, more preferably 8 or more carbon atoms, and preferably 18 or less carbon atoms, and more preferably 16 or less carbon atoms. The alkyl group of the alkyl sulfosuccinate may be either linear or branched, and from the viewpoint of improving digestibility, the alkyl group may be preferably linear. The alkyl sulfosuccinate may be a diester type compound having preferably two alkyl groups (hereinafter referred to as dialkyl sulfosuccinate). From the viewpoint of improving digestibility, the dialkyl sulfosuccinate may have a total carbon number of preferably 10 or more carbon atoms, more preferably 12 or more carbon atoms, and preferably 24 or less carbon atoms, and more preferably 20 or less carbon atoms. The carbon numbers of the two alkyl groups of the dialkyl sulfosuccinate may be the same or different. Examples of salts constituting the alkyl sulfosuccinate and the dialkyl sulfosuccinate include alkali metal salts such as sodium and potassium, and from the viewpoint of improving digestibility, the salt may be preferably a sodium salt.

[0018] Examples of salts constituting the cholate include alkali metal salts such as sodium and potassium salts, and from the viewpoint of improving digestibility, the sodium salt may be preferred.

[0019] From the viewpoint of improving digestibility, the component (A) may be preferably sodium lauryl sulfate or thiamine lauryl sulfate, and from the viewpoint of improving intake, the component (A) may be preferably thiamine lauryl sulfate.

[0020] The amount of each component in the feed additive composition of the present invention is determined based on the total amount of protein in the feed to which the composition is added. From the viewpoint of improving digestibility, the content of component (A) is preferably 0.1 parts by mass or more, more preferably 0.2 parts by mass or more, even more preferably 0.5 parts by mass or more, even more preferably 1 part by mass or more, even more preferably 2 parts by mass or more, even more preferably 3 parts by mass or more, and from the viewpoint of improving feed intake and economic efficiency, it may be preferably 50 parts by mass or less, more preferably 40 parts by mass or less, even more preferably 30 parts by mass or less, even more preferably 20 parts by mass or less, even more preferably 15 parts by mass or less. The amount of the anionic surfactant in component (A) is based on the compound in which the counter ion is replaced with a sodium ion.

[0021] The feed additive composition of the present invention contains component (B), that is, a nonionic surfactant.

[0022] From the viewpoint of improving digestibility, the (B) component may be a nonionic surfactant having an HLB of preferably 6 or more and 18 or less. From the viewpoint of improving digestibility, the (B) component may have an HLB of preferably 6 or more, more preferably 8 or more, even more preferably 10 or more, still more preferably 13 or more, and preferably 18 or less, more preferably 17 or less, and even more preferably 16.5 or less.

[0023] In the present invention, nonionic surfactants having an HLB of 10 or more and an HLB of 17 or less are preferred.

[0024] The HLB value of component (B) can be calculated using the Griffin formula shown below. HLB = 20 x (sum of formula weights of hydrophilic parts of nonionic surfactants) / (molecular weight of nonionic surfactant) When two or more types of nonionic surfactants are contained, the HLB of component (B) is calculated as a weighted average.

[0025] Examples of component (B) include one or more selected from polyoxyethylene alkyl ethers, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, sucrose fatty acid esters, glycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, and polyoxyethylene hydrogenated castor oil.

[0026] In the polyoxyethylene alkyl ether, from the viewpoint of improving digestibility, the alkyl group may preferably have 8 or more carbon atoms, more preferably 10 or more carbon atoms, and preferably have 20 or less carbon atoms, more preferably 18 or less carbon atoms, and even more preferably have 16 or less carbon atoms. In the polyoxyethylene alkyl ether, the alkyl group may be either linear or branched, and from the viewpoint of improving digestibility, the alkyl group may preferably be linear. In the polyoxyethylene alkyl ether, from the viewpoint of improving digestibility, the average number of moles of ethylene oxide added may be preferably 2 or more, more preferably 8 or more, and preferably 50 or less, more preferably 45 or less, and even more preferably 40 or less.

[0027] In the present invention, polyoxyethylene alkyl ethers having an alkyl group having 10 or more and 16 or less carbon atoms and an average added mole number of ethylene oxide of 8 or more and 40 or less are preferred.

[0028] From the viewpoint of improving digestibility, the fatty acid in the fatty acid-derived structural unit constituting the sorbitan fatty acid ester and the polyoxyethylene sorbitan fatty acid ester may preferably have 8 or more carbon atoms, more preferably 10 or more carbon atoms, and preferably 20 or less carbon atoms, more preferably 18 or less carbon atoms. The fatty acid in the fatty acid-derived structural unit constituting the sorbitan fatty acid ester and the polyoxyethylene sorbitan fatty acid ester may be either a straight chain or a branched chain, and preferably a straight chain fatty acid. The fatty acid may be either a saturated or unsaturated fatty acid, and a mixed fatty acid may be used.

[0029] The esters constituting the sorbitan fatty acid ester and the polyoxyethylene sorbitan fatty acid ester include monoesters, diesters and triesters, and are preferably monoesters.

[0030] The average number of moles of ethylene oxide added in the polyoxyethylene sorbitan fatty acid ester may be preferably 2 or more, more preferably 5 or more, and preferably 40 or less, more preferably 25 or less, from the viewpoint of improving digestibility.

[0031] In the present invention, polyoxyethylene sorbitan fatty acid (mono)esters composed of straight-chain fatty acids having 10 or more and 18 or less carbon atoms and having an average added mole number of ethylene oxide of 5 or more and 25 or less are preferred.

[0032] From the viewpoint of improving digestibility, the fatty acid in the fatty acid-derived structural unit constituting the polyoxyethylene fatty acid ester may preferably have 6 or more carbon atoms, more preferably 8 or more carbon atoms, and preferably have 20 or less carbon atoms, more preferably have 18 or less carbon atoms. The fatty acid in the fatty acid-derived structural unit constituting the polyoxyethylene fatty acid ester may be either a straight-chain or branched-chain fatty acid, and preferably a straight-chain fatty acid. The fatty acid may be either a saturated or unsaturated fatty acid, and a mixed fatty acid may be used. From the viewpoint of improving digestibility, the average number of moles of ethylene oxide added in the polyoxyethylene fatty acid ester may be preferably 2 or more, more preferably 10 or more, and preferably 50 or less, more preferably 40 or less.

[0033] In the present invention, polyoxyethylene fatty acid esters composed of straight-chain fatty acids having 8 or more and 18 or less carbon atoms and having an average added mole number of ethylene oxide of 10 or more and 40 or less are preferred.

[0034] From the viewpoint of improving digestibility, the fatty acid in the fatty acid-derived structural unit constituting the sucrose fatty acid ester may preferably have 8 or more carbon atoms, more preferably 10 or more carbon atoms, and preferably 20 or less carbon atoms, more preferably 18 or less carbon atoms. The fatty acid in the fatty acid-derived structural unit constituting the sucrose fatty acid ester may be either a straight-chain or branched-chain fatty acid, and preferably a straight-chain fatty acid. The fatty acid may be either a saturated or unsaturated fatty acid, and a mixed fatty acid may be used.

[0035] In the present invention, sucrose fatty acid esters composed of straight-chain fatty acids having 8 or more carbon atoms and 18 or less carbon atoms are preferred.

[0036] The fatty acid in the fatty acid-derived structural unit constituting the glycerin fatty acid ester and polyoxyethylene glycerin fatty acid ester may preferably have 6 or more carbon atoms, more preferably 8 or more carbon atoms, and preferably 20 or less carbon atoms, more preferably 18 or less carbon atoms, from the viewpoint of improving digestibility. The fatty acid in the fatty acid-derived structural unit constituting the glycerin fatty acid ester and polyoxyethylene glycerin fatty acid ester may be either a straight chain or a branched chain, preferably a straight chain fatty acid, from the viewpoint of improving digestibility. The fatty acid may be either a saturated or unsaturated fatty acid, and a mixed fatty acid may be used. The ester constituting the glycerin fatty acid ester and polyoxyethylene glycerin fatty acid ester may be a monoester, a diester, or a triester, and may preferably be a monoester.

[0037] The average number of moles of ethylene oxide added in the polyoxyethylene glycerin fatty acid ester may be preferably 2 or more, more preferably 10 or more, and preferably 50 or less, more preferably 40 or less, from the viewpoint of improving digestibility.

[0038] In the present invention, polyoxyethylene glycerin fatty acid esters composed of straight-chain fatty acids having 8 or more carbon atoms and 18 or less carbon atoms and having an average added mole number of ethylene oxide of 10 or more and 40 or less are preferred.

[0039] From the viewpoint of improving digestibility, the average number of moles of ethylene oxide added in polyoxyethylene hydrogenated castor oil is preferably 2 or more, more preferably 10 or more, even more preferably 20 or more, and preferably 50 or less, even more preferably 45 or less.

[0040] From the viewpoint of improving digestibility, the component (B) is preferably a nonionic surfactant having ethylene oxide and an HLB value of 6 or more and 18 or less, and examples thereof include polyoxyethylene alkyl ethers, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, etc. From the viewpoint of improving digestibility, preferred examples thereof include polyoxyethylene alkyl ethers, polyoxyethylene sorbitan fatty acid esters, and polyoxyethylene glycerin fatty acid esters.

[0041] Specific examples of the component (B) include polyoxyethylene lauryl ether, sorbitan laurate, polyoxyethylene sorbitan laurate, polyoxyethylene sorbitan oleate, and capric acid monoglyceride.

[0042] As the (B) component, from the viewpoint of improving digestibility, preferable examples include polyoxyethylene lauryl ether and polyoxyethylene sorbitan oleate, and from the viewpoint of improving intake, preferable examples include polyoxyethylene sorbitan laurate, polyoxyethylene sorbitan oleate, and capric acid monoglyceride.

[0043] The amount of each component in the feed additive composition of the present invention is determined based on the total amount of protein in the feed to which the composition is added. From the viewpoint of improving digestibility, the content of component (B) is preferably 0.1 parts by mass or more, more preferably 0.2 parts by mass or more, even more preferably 0.5 parts by mass or more, still more preferably 1 part by mass or more, and still more preferably 2 parts by mass or more, per 100 parts by mass of protein in the feed, and from the viewpoints of improving intake and economic efficiency, it may be 20 parts by mass or less, preferably 15 parts by mass or less.

[0044] From the viewpoint of improving digestibility, the feed additive composition of the present invention may have a mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] of 0.7 or more, preferably 0.9 or more, more preferably 1.0 or more, even more preferably 2.0 or more, and may be 20 or less, preferably 10 or less, and even more preferably 6 or less. From the viewpoint of improving feed intake, the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] may be 20 or less, preferably 10 or less, more preferably 8 or less, and even more preferably 5.0 or less.

[0045] The feed additive composition of the present invention may be prepared by mixing the anionic surfactant as component (A) and the nonionic surfactant as component (B) in a mass ratio [(A) / (B)] of 0.7 or more and 20 or less.

[0046] The feed additive composition of the present invention may be in either solid or liquid form.

[0047] [Feed composition] The feed composition of the present invention contains the above-mentioned feed additive composition and a protein. The feed composition of the present invention is a feed composition containing (A) an anionic surfactant, (B) a nonionic surfactant, and (C) a protein, and the mass ratio of the content of the component (A) to the content of the component (B) [(A) / (B)] is 0.7 to 20. Specific examples and preferred examples of the components (A) and (B) of the feed composition of the present invention may be the same as those of the feed additive composition of the present invention described above.

[0048] The protein of component (C) may be a vegetable protein or an animal protein, or may contain both vegetable and animal proteins. Examples of the protein source of vegetable protein include soybean meal, corn gluten meal, corn, milo, barley, wheat, cassava, bran, rice bran, rapeseed oil cake, feed rice, etc., and are not particularly limited, but from the viewpoint of economy, soybean meal may be preferable. Examples of the protein source of animal protein include fish meal, adjusted fish meal, fish meal, skim milk powder, whey powder, chicken meal, feather meal, meat meal, meat and bone meal, etc.

[0049] The feed composition of the present invention may preferably contain a vegetable protein as component (C), and is preferably a vegetable protein-containing feed composition.

[0050] The feed composition of the present invention may preferably contain a vegetable protein and an animal protein as component (C). The feed composition of the present invention can contain, in the protein contained in the composition, i.e., the animal protein in component (C), from the viewpoint of improving growth performance, preferably 5% by mass or more, more preferably 10% by mass or more, even more preferably 20% by mass or more, and from the viewpoint of economy, preferably 80% by mass or less, more preferably 60% by mass or less, even more preferably 50% by mass or less.

[0051] When the feed composition of the present invention contains a vegetable protein and an animal protein as component (C), the mass ratio of the animal protein content to the total amount of protein in the feed composition [animal protein / protein] may be, for example, 0.1 or more, more preferably 0.2 or more, and even more preferably 0.4 or more, from the viewpoint of improving digestibility, and may be 0.8 or less, preferably 0.7 or less, and more preferably 0.6 or less, from the viewpoint of economy.

[0052] From the viewpoint of growth potential, the feed composition of the present invention may contain component (C) in an amount of preferably 10% by mass or more, more preferably 20% by mass or more, even more preferably 30% by mass or more, still more preferably 40% by mass or more, and preferably 99% by mass or less, more preferably 97% by mass or less, even more preferably 90% by mass or less, still more preferably 80% by mass or less, still more preferably 70% by mass or less, and still more preferably 60% by mass or less.

[0053] From the viewpoint of improving digestibility, the feed composition of the present invention may contain, per 100 parts by mass of component (C), preferably 0.1 part by mass or more, more preferably 0.2 parts by mass or more, even more preferably 0.5 parts by mass or more, still more preferably 1 part by mass or more, still more preferably 2 parts by mass or more, still more preferably 3 parts by mass or more of component (A); and from the viewpoint of improving feed intake and economy, the feed composition may contain 50 parts by mass or less, preferably 40 parts by mass or less, even more preferably 30 parts by mass or less, still more preferably 20 parts by mass or less, and still more preferably 15 parts by mass or less.

[0054] From the viewpoint of improving digestibility, the feed composition of the present invention may contain 0.1 part by mass or more of component (B) per 100 parts by mass of component (C), preferably 0.2 parts by mass or more, more preferably 0.5 parts by mass or more, even more preferably 1 part by mass or more, and even more preferably 2 parts by mass or more, and from the viewpoint of improving feed intake and economic efficiency, the feed composition may contain 20 parts by mass or less, preferably 15 parts by mass or less.

[0055] From the viewpoint of improving digestibility, the feed composition of the present invention may have a mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] of 0.7 or more, preferably 0.9 or more, more preferably 1.0 or more, even more preferably 2.0 or more, and may have a mass ratio of 20 or less, preferably 10 or less, and more preferably 6 or less. From the viewpoint of improving feed intake, the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] may have a mass ratio of 20 or less, preferably 10 or less, more preferably 8 or less, even more preferably 5.0 or less, and even more preferably 4.0 or less.

[0056] The feed composition of the present invention may contain the following optional components in addition to components (A), (B), and (C): Examples include excipients such as starch and carboxymethylcellulose, ash such as vitamins (excluding vitamin B1) and minerals, dietary fiber, lipids, carbohydrates, yeasts such as torula yeast, amino acids such as methionine, etc. The optional components may include surfactants other than components (A) and (B), i.e., cationic surfactants, amphoteric surfactants, etc.

[0057] In the feed composition of the present invention, the total content of component (A) and component (B) in 100% by mass of all the surfactants contained in the feed composition may be preferably 80% by mass or more, more preferably 90% by mass or more, and even more preferably 95% by mass or more.

[0058] The feed composition of the present invention may be produced by mixing components (A) and (B) with component (C) in a mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] in the range of 0.7 to 20.

[0059] Furthermore, the feed composition of the present invention may contain 0.1 to 50 parts by mass of component (A) and 0.1 to 20 parts by mass of component (B) relative to 100 parts by mass of component (C), and the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is from 0.7 to 20, and may be produced by adding other components as necessary and shaping, etc.

[0060] The feed composition of the present invention may be in any form, including, but not limited to, a solid form, particularly, a pellet form, a granule form, or a powder form. Examples of the pellet form include moist pellets, dry pellets, single moist pellets, and extruded pellets.

[0061] When the feed composition of the present invention is a solid feed composition for farmed fish, the particle size (major axis) of the solid feed composition can be adjusted depending on the target fish species, but may be, for example, preferably 0.5 mm or more, more preferably 1.0 mm or more.

[0062] When the solid feed composition of the present invention is a single moist pellet or moist pellet, the water content in 100% by mass of the composition is preferably 10% by mass or more, more preferably 20% by mass, from the viewpoint of improving shape retention and granulation properties, and preferably 70% by mass or less, more preferably 60% by mass or less, from the viewpoint of improving storage stability and economical efficiency. When the solid feed composition of the present invention is a dry pellet or extruded pellet, the water content in 100% by mass of the composition is preferably 1% by mass or more, more preferably 3% by mass, from the viewpoint of improving shape retention and granulation properties, and preferably 40% by mass or less, more preferably 30% by mass or less, from the viewpoint of improving storage stability and economical efficiency.

[0063] The feed composition of the present invention may preferably be a fish feed composition. The fish and shellfish to which the fish feed composition of the present invention is fed are preferably fish, such as, but not limited to, yellowtail, yellowtail, red sea bream, amberjack, tuna, flounder, striped jack, horse mackerel, tiger puffer, yellowtail amberjack, stone bream, filefish, black porgy, grouper, chub mackerel, and spotted grouper.

[0064] [Fish and shellfish farming methods] The present invention provides a method for cultivating fish and shellfish, which comprises feeding the feed composition to farmed fish and shellfish. The method for cultivating fish and shellfish in the present invention may be a typical method for cultivating fish and shellfish, except that the fish and shellfish are fed a fish feed containing the feed additive composition of the present invention and the feed composition of the present invention.

[0065] [Protein digestion promoter composition] The present invention has discovered that when an anionic surfactant (A) and a nonionic surfactant (B) are contained in a composition containing protein such that the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less, digestion of protein ingested from feed can be promoted in livestock such as seafood, chicken, pig, and cow. That is, the present invention provides a protein digestion promoter composition containing an anionic surfactant (A) and a nonionic surfactant (B), in which the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less. The protein digestion promoter composition of the present invention may be used for fish farming, chicken farming, pig farming, and cow farming, and may be preferably used for fish farming.

[0066] The protein digestion promoter composition of the present invention may be a vegetable protein digestion promoter composition, a fish farming protein digestion promoter composition, or a vegetable protein digestion promoter composition for fish farming. In the promoter composition of the present invention, specific examples and preferred examples of the (A) component and the (B) component may be the same as those of the feed additive composition and the feed composition of the present invention described above.

[0067] As shown in the Examples below, the protein digestion promoter composition of the present invention can be confirmed to function as a protein digestion promoter composition by, for example, examining the protein degradation rate in in vitro or in vivo tests. The protein digestion promoter composition of the present invention can be prepared in the same manner as the feed additive composition of the present invention.

[0068] The present invention discloses the following feed additive composition, feed composition, fish and shellfish farming method, and protein digestion promoter composition.

[0069] <1> A feed additive composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)] and (B) a nonionic surfactant [hereinafter referred to as component (B)], wherein the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B)] is 0.7 or more and 20 or less.

[0070] <2> The component (A) is an anionic surfactant selected from alkyl sulfates, alkyl benzene sulfonates, alkyl sulfosuccinates, and cholates. <1> The feed additive composition according to claim 1.

[0071] <3> The feed contains the component (A) in an amount of 0.1 parts by mass or more and 50 parts by mass or less per 100 parts by mass of protein in the feed. <1> or <2> The feed additive composition according to claim 1.

[0072] <4> (B) component has an HLB value of 6 or more and 18 or less. <1> ~ <3> 13. The feed additive composition according to claim 12,

[0073] <5> The above, wherein the component (B) is a nonionic surfactant selected from polyoxyethylene alkyl ethers, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, sucrose fatty acid esters, glycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, and polyoxyethylene hydrogenated castor oil. <1> ~ <4> 13. The feed additive composition according to claim 12,

[0074] <6> The feed contains the component (B) in an amount of 0.1 parts by mass or more and 20 parts by mass or less per 100 parts by mass of protein in the feed. <1> ~ <5> 13. The feed additive composition according to claim 12,

[0075] <7> The above-mentioned is for use as feed for fish and shellfish farming. <1> ~ <6> 13. The feed additive composition according to claim 12,

[0076] <8> A feed composition comprising (A) an anionic surfactant (hereinafter referred to as component (A)), (B) a nonionic surfactant (hereinafter referred to as component (B)) and (C) a protein (hereinafter referred to as component (C)), wherein the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is 0.7 or more and 20 or less.

[0077] <9> The component (A) is an anionic surfactant selected from alkyl sulfates, alkyl benzene sulfonates, alkyl sulfosuccinates, and cholates. <8> The feed composition described.

[0078] <10> The composition contains the component (A) in an amount of 0.1 parts by mass or more and 50 parts by mass or less per 100 parts by mass of the component (C). <8> or <9> The feed composition according to claim 1.

[0079] <11> (B) component has an HLB value of 6 or more and 18 or less. <8> ~ <10> 13. A feed composition according to any one of the above.

[0080] <12> The above, wherein the component (B) is a nonionic surfactant selected from polyoxyethylene alkyl ethers, sorbitan fatty acid esters, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene fatty acid esters, sucrose fatty acid esters, glycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, and polyoxyethylene hydrogenated castor oil. <8> ~ <11> 13. A feed composition according to any one of the above.

[0081] <13> The composition contains the component (B) in an amount of 0.1 parts by mass or more and 20 parts by mass or less per 100 parts by mass of the component (C). <8> ~ <12> 13. A feed composition according to any one of the above.

[0082] <14> The component (C) contains a vegetable protein. <8> ~ <13> 13. A feed composition according to any one of the above.

[0083] <15> The above-mentioned is a feed for fish and shellfish farming. <8> ~ <14> 13. A feed composition according to any one of the above.

[0084] <16> The above <8> ~ <15> A method for cultivating fish and shellfish, comprising feeding the fish and shellfish with the feed composition described in any one of the above.

[0085] <17> A protein digestion promoter composition for farmed fish containing (A) an anionic surfactant (hereinafter referred to as component (A)), (B) a nonionic surfactant (hereinafter referred to as component (B)) and (C) a protein (hereinafter referred to as component (C)), wherein the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B) is 0.7 or more and 20 or less.

[0086] <18> A method for producing a feed composition, comprising mixing (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)] in such a manner that the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is in the range of 0.7 to 20.

[0087] <19> A method for farming fish and shellfish, comprising feeding farmed fish and shellfish a feed composition containing (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)], in such a manner that the mass ratio of the content of component (A) to the content of component (B) [(A) / (B)] is in the range of 0.7 to 20. EXAMPLES

[0088] <Example 1 and Comparative Example 1> [Protein digestibility (in vitro test)] (1) Preparation of feed composition A feed composition was prepared by adding the (A) and (B) components shown in Table 1 in the amounts shown in Table 1 to soybean meal (manufactured by Showa Sangyo Co., Ltd., containing 43% by mass of protein as component (C)) as a protein source and stirring in a mortar for about 5 minutes.

[0089] (2) Preparation of red sea bream extract enzyme Intestines were collected from red sea bream (Pagrus major), and purified water was added at a mass ratio of 1:1, which was then crushed in a mixer and filtered. The resulting filtrate was used as the red sea bream extract enzyme.

[0090] (3) Calculation of protein degradation rate In a 30 mL eggplant flask, 10 mg of the feed composition prepared in (1) as a protein derived from soybean meal, 50 μL of red sea bream extract enzyme, and 5 mL of phosphate buffer (pH 8) were added and stirred to prepare a crude treatment liquid. After 24 hours, 100 μL of the crude treatment liquid was placed in a sampling tube (1.5 mL capacity), and the sampling tube was immersed in boiling water and boiled for 5 minutes so that the entire amount of the crude treatment liquid in the sampling tube was heated. The heated sampling tube was centrifuged (10,000 rpm, 5 minutes) to deproteinize the crude treatment liquid, and a protein digestion treatment liquid (hereinafter, treatment liquid) was obtained.

[0091] Trinitrobenzenesulfonic acid (TCI) was added to the treatment solution and reacted at 60° C. for 1 hour, and hydrochloric acid was added and stirred to stop the reaction. The absorbance of the resulting treatment solution was measured at 420 nm. The absorbance at 420 nm was measured, and a calibration curve was drawn with tyrosine to calculate the protein degradation rate. The protein digestibility was evaluated as a relative value to the protein degradation rate in Comparative Example 1. The results are shown in Table 1. A higher digestibility value in Table 1 means that protein digestion can be promoted.

[0092] [Table 1] *1: Parts by weight per 100 parts by weight of protein source (soybean meal) *2: Content when 43% by mass of (C) protein contained in protein source (soybean meal) is taken as 100 parts by mass. *3: Relative value when Comparative Example 1-1 is set to 100

[0093] The components in the table are as follows. (B) The HLB of the component is calculated by the Griffin method.

[0094] <Composition ingredients> Component (A): Anionic surfactant Sodium lauryl sulfate: Emal 10PT, Kao Corporation Ammonium lauryl sulfate: Emeral AD-25R, Kao Corporation Thiamine lauryl sulfate: Fine VB-100, Tsuruya Chemical Industry Co., Ltd. Sodium laurylbenzenesulfonate: Neoperex G20, Kao Corporation Sodium cholate: Fujifilm Wako Pure Chemical Corporation

[0095] (B) Component: Nonionic surfactant POE(2)C12 ether: Polyoxyethylene(2) lauryl ether (numbers in parentheses are average number of moles added, same below), Kao Corporation (HLB6.3) POE(12)C12 Ether: Polyoxyethylene(12) Lauryl Ether, Kao Corporation (HLB15.3) POE(43)C12 Ether: Polyoxyethylene(43) Lauryl Ether, Kao Corporation (HLB16.3) C12 sorbitan fatty acid ester: Rheodol SP-L10, Kao Corporation (HLB 8.6) C12EO(6) Sorbitan fatty acid ester: Rheodol TW-L106, Kao Corporation (HLB13.3) C12EO(20) Sorbitan fatty acid ester: Rheodol TW-L120, Kao Corporation (HLB16.7) C18:1EO(6) Sorbitan fatty acid ester: Rheodol TW-O106, Kao Corporation (HLB10) C18:1EO(20) Sorbitan fatty acid ester: Rheodol TW-O120, Kao Corporation (HLB15) C10 monoglyceride: Sunsoft 760H, Taiyo Kagaku Co., Ltd. (HLB 6.5)

[0096] <Example 2 and Comparative Example 2> [Protein digestibility (in vitro test)] The feed composition was prepared, the red sea bream extract enzyme was prepared, and the protein decomposition rate was calculated in the same manner as in Example 1 and Comparative Example 1, except that the soybean meal, which was the protein source in Example 1, was replaced with the protein source shown in Table 2, and the components (A) and (B) were added in the amounts shown in Table 2. The components (A) and (B) used were the same as those in Example 1 and Comparative Example 1. The results are shown in Table 2.

[0097] [Table 2] *1: Parts by weight per 100 parts by weight of protein source *2: Content when component (C) (pure protein) contained in protein source is taken as 100 parts by mass *3: Relative value when Comparative Example 2-n is set to 100 (n = 1 to 5)

[0098] <Example 3 and Comparative Example 3> [Growth (In vivo test)] (1) Preparation of feed composition To a mixture of 25 g of soybean meal (manufactured by Showa Sangyo Co., Ltd., containing 43% by mass of protein as component (C)) as a protein source and 40 g of fish meal (manufactured by Yoshida Feed Co., Ltd., containing 61% by mass of protein as component (C)), 50 g of tap water and the components (A) and (B) shown in Table 3 were added in the amounts shown in Table 3. After thorough mixing, the mixture was molded using a single-axis type extrusion granulator (rotation speed: 50 rpm) with a screen diameter of 3 mm to mold single moist pellets. The moisture content was 42 to 44%. The components (A) and (B) used were the same as those used in Example 1 and Comparative Example 1. A halogen moisture meter (manufactured by Mettler) was used to measure the moisture content, and the moisture content in 1 g of feed was measured.

[0099] (2) Feeding method of the feed composition Red sea bream fry of about 5 cm in size were used, and were raised for 28 days, starting from day 0, with feeding twice a day in the morning and evening, under conditions of a water temperature of 20°C ± 1.5°C. The fed red sea bream fry ingested all of the feed compositions of the present invention without any problems. It was also confirmed that the red sea bream fry ingesting the feed composition of the present invention could grow without suffering from disorders such as diarrhea.

[0100] (3) Measurement of weight gain rate The weight of the fish was measured on day 0 and on day 28. The weight gain rate was calculated using the following formula: Weight Gain Rate = {(weight on the 28th day) / (weight on the 0th day) × 100} - 100

[0101] [Table 3] *1: Parts by weight per 100 parts by weight of protein source (mixture of soybean meal and fish meal) *2: Content when component (C) (pure protein) contained in protein source is taken as 100 parts by mass *3: Relative value when Comparative Example 3-n is set to 100 (n = 1 to 5)

Claims

1. A feed additive composition comprising (A) an anionic surfactant [hereinafter referred to as component (A)] and (B) a nonionic surfactant [hereinafter referred to as component (B)].

2. The feed additive composition according to Claim 1, wherein the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B) is 0.7 or more and 20 or less.

3. The feed additive composition according to claim 1 or 2, wherein component (A) is an anionic surfactant selected from alkyl sulfates, alkylbenzene sulfonates, alkyl sulfosuccinates, and cholates.

4. The feed additive composition according to claim 1 or 2, wherein the HLB of component (B) is 6 or more and 18 or less.

5. A feed composition containing (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) protein [hereinafter referred to as component (C)].

6. The feed composition according to claim 5, wherein the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B) is 0.7 or more and 20 or less.

7. The feed composition according to claim 5 or 6, wherein component (A) is an anionic surfactant selected from alkyl sulfates, alkylbenzene sulfonates, alkyl sulfosuccinates, and cholates.

8. The feed composition according to claim 5 or 6, wherein component (A) is contained in an amount of 0.1 parts by mass or more and 50 parts by mass or less per 100 parts by mass of component (C).

9. The feed composition according to claim 5 or 6, wherein the HLB of component (B) is 6 or more and 18 or less.

10. The feed composition according to claim 5 or 6, wherein component (B) is contained in an amount of 0.1 parts by mass or more and 20 parts by mass or less per 100 parts by mass of component (C).

11. The feed composition according to claim 5 or 6, wherein component (C) contains plant protein.

12. The feed composition according to claim 5 or 6, which is a feed for aquaculture of fish and shellfish.

13. A protein digestion accelerator composition for aquaculture, comprising (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)].

14. The protein digestion promoter composition for aquaculture according to claim 13, wherein the mass ratio [(A) / (B)] of the content of component (A) to the content of component (B) is 0.7 or more and 20 or less.

15. The aquaculture protein digestion accelerator composition according to claim 13 or 14, wherein component (A) is an anionic surfactant selected from alkyl sulfates, alkylbenzene sulfonates, alkyl sulfosuccinates, and cholates.

16. The protein digestion accelerator composition for aquaculture according to claim 13 or 14, wherein the HLB of component (B) is 6 or more and 18 or less.

17. A method for producing a feed composition, comprising mixing (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) a protein [hereinafter referred to as component (C)].

18. A method for farming fish and shellfish, comprising feeding farmed fish and shellfish a feed composition containing (A) an anionic surfactant [hereinafter referred to as component (A)], (B) a nonionic surfactant [hereinafter referred to as component (B)], and (C) protein [hereinafter referred to as component (C)].

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