Intestinal epithelial-like cells and production method thereof
Culturing intestinal organoids in a monolayer to produce intestinal epithelial-like cells with enhanced drug-metabolizing enzyme and transporter expression addresses the limitations of current methods, enabling efficient pharmacokinetic and drug toxicity evaluations.
JP2024175107AActive Publication Date: 2024-12-17OSAKA UNIVERSITY +1
Patent Information
- Application Number
- JP2024162329
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Filing Date
- 2024-09-19
- Publication Date
- 2024-12-17
- Estimated Expiration
- 2040-02-03
Smart Images
Figure 2024175107000001_ABST
Abstract
To provide excellent intestinal epithelial-like cells expressing drug-metabolizing enzymes and drug transporters applicable for pharmacokinetic assessment, more specifically, intestinal epithelial-like cells having properties closer to those of primary cultured human intestinal epithelial cells that are difficult to obtain, and to provide production methods thereof.SOLUTION: The present invention is characterized by the monolayer culturing of organoid-derived cells. As a result of analyzing the obtained monolayer film, drug-metabolizing enzymes (each CYP) and drug transporters (PEPT1, MDR1), and the like possessed by small intestinal epithelial cells were expressed and excellent intestinal epithelial-like cells that also have a tight junction function were obtained. From the above, intestinal epithelial-like cells that can be used for pharmacokinetic evaluation and / or drug toxicity evaluation can be efficiently produced.SELECTED DRAWING: Figure 1
Need to check novelty before this filing date? Find Prior Art
Citation Information
Patent Citations
Methods of Generating Gastrointestinal Epithelial Tissue Constructs Nancy Allbritton, Yuli Wang, Christopher Sims, Scott Magness and Scott Bultman
JP2018506971A
Method for producing pluripotent stem cell-derived intestinal organoid
WO2020091020A1
Intestinal epithelioid cells
WO2016147975A1
2d organoid for infection and culture of human diarrhea virus, and use of said 2d organoid
WO2018038042A1