Control of multiple gene expression using synthetic promoters.
Patent Information
- Application Number
- JP2023556871
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2021-03-16
- Filing Date
- 2022-03-15
- Publication Date
- 2025-03-18
AI Technical Summary
Current methods for controlling the stoichiometry and co-expression of multiple recombinant genes in mammalian cells are limited by the number of genes that can be regulated, require multiple plasmids, and often impose metabolic burdens or cellular stress, making them complex and imprecise.
The use of synthetic promoters with defined transcriptional activity, linked to transcription factor regulatory elements, allows for precise control of multiple gene expression in multigene expression vectors, enabling predictable stoichiometry and reduced cellular stress.
The solution provides precise control over the expression levels of multiple genes, reducing cellular stress and metabolic burden, while allowing for predictable and efficient expression of recombinant proteins.