Method for cultivating sprouts

Abstract

To provide a method for cultivating sprouts that is capable of promoting the synthesis of at least one of ascorbic acid and glutamic acid, which are functional components contained in the sprouts.SOLUTION: A method for cultivating sprouts includes at least one of: a UV irradiation step in which ultraviolet rays are applied onto a plant body at an output of 0.4 mW / cm2 or more; and an ultrasonic treatment step in which ultrasonic waves are applied onto a seed at a frequency of 30 kHz or less.SELECTED DRAWING: None

Description

Technical Field

[0001] The present disclosure relates to a method for cultivating sprouts.

Background Art

[0002] Sprouts, which are new shoots germinated from plant seeds, have been consumed as a common traditional food around the world for many years. In recent years, there has been a demand for cultivating sprouts containing components (hereinafter referred to as "functional components") that are said to be effective in preventing lifestyle-related diseases such as vitamins.

[0003] Patent Document 1 describes a method for increasing polyphenols contained in baby leaves and sprouts by irradiating specific visible light and ultraviolet light. Patent Document 2 describes a method for increasing carotene and vitamin A contained in soybean sprouts by irradiating near ultraviolet light to natural light during a specific growth period after the germination of soybean sprouts. Patent Document 3 describes a method for improving the visible green color of soybean sprouts by irradiating ultraviolet light after the germination of soybean sprouts.

[0004] Non-Patent Document 1 describes a method for increasing polyphenols contained in radish sprouts or improving DPPH radical scavenging activity by irradiating ultrasonic waves to the roots in the hydroponic cultivation of radish sprouts. Non-Patent Document 2 describes a method for improving the germination rate or increasing isoflavonoids and GABA contained in bean sprouts by ultrasonic treatment of bean sprouts. Non-Patent Document 3 describes a method for promoting germination or increasing total isoflavonoids and total anthocyanins contained in bean sprouts by ultrasonic treatment of bean sprouts.

Prior Art Documents

Patent Documents

[0005]

Patent Document 1

Patent Document 2

Patent Document 3

Non-Patent Document

[0006]

Non-Patent Document 1

Non-Patent Document 2

Non-Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0007] As described above, various studies have been conducted on the cultivation of sprouts, but further development of technology regarding the diversity of functional components contained in sprouts is desired.

[0008] The present disclosure has been made in view of the above. An object of the present disclosure is to provide a method for cultivating sprouts that can promote the synthesis of at least one of the functional components, ascorbic acid and glutamic acid, contained in sprouts.

Means for Solving the Problems

[0009] Specific means for solving the above problems include the following aspects. <1> Irradiate the plant body with ultraviolet light at 0.4 mW / cm 2A method for cultivating sprouts, comprising at least one of an ultraviolet irradiation step of irradiating with the above output and an ultrasonic treatment step of irradiating the seeds with ultrasonic waves at a frequency of 30 kHz or less. <2> The method for cultivating sprouts according to <1> above, wherein the ultrasonic treatment step is a step of irradiating the seeds with ultrasonic waves at a frequency of 1 kHz to 30 kHz for 5 minutes to 40 minutes. <3> The method for cultivating sprouts according to <1> or <2> above, wherein the ultrasonic treatment step is a step of irradiating the seeds with ultrasonic waves at a frequency of 10 kHz to 30 kHz. <4> The ultraviolet irradiation step is a step of irradiating the plant body with ultraviolet rays at an output of 3.0 mW / cm 2 ~5.0 mW / cm 2 The method for cultivating sprouts according to any one of <1> to <3> above. <5> The method for cultivating sprouts according to any one of <1> to <4> above, wherein the sprouts are mung bean sprouts.

Advantages of the Invention

[0010] According to the present disclosure, there is provided a method for cultivating sprouts that can promote the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprouts.

Brief Description of the Drawings

[0011]

Figure 1

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MODE FOR CARRYING OUT THE INVENTION

[0012] Hereinafter, an embodiment of the present disclosure will be described in detail. However, the present disclosure is not limited to the following embodiments. In the following disclosure, its components (including element steps, etc.) are not essential unless otherwise specified. The same applies to numerical values and their ranges, which do not limit the present disclosure.

[0013] In the present disclosure, the term "step" includes, in addition to steps independent of other steps, also those steps that, even if they cannot be clearly distinguished from other steps, are included as long as the purpose of the step is achieved. In the numerical range indicated by "~" in the present disclosure, the numerical values described before and after "~" are included as the lower limit value and the upper limit value, respectively. In the numerical ranges described step by step in the present disclosure, the upper limit value or the lower limit value described in one numerical range may be replaced with the upper limit value or the lower limit value of the numerical range described in other step-by-step descriptions. Also, in the numerical ranges described in the text, the upper limit value or the lower limit value of the numerical range may be replaced with the value shown in the examples. In the present disclosure, the content rate of each component in the composition means the total content rate of the plurality of substances corresponding to each component in the composition, unless otherwise specified, when there are a plurality of substances corresponding to each component in the composition.

[0014] ≪Method for Cultivating Sprouts≫ The method for cultivating sprouts of the present disclosure includes at least one of an ultraviolet irradiation step of irradiating with ultraviolet rays (UV) at an output of 0.4 mW / cm 2 or more, and an ultrasonic treatment step of irradiating seeds with ultrasonic waves at a frequency of 30 kHz or less.

[0015] The sprout cultivation method of the present disclosure includes an ultraviolet irradiation step of irradiating with UV at a specific output and an ultrasonic treatment step of irradiating seeds with ultrasonic waves of a specific wavelength. Thereby, it is considered that since the sprouts obtain an appropriate stimulus during the growth process, the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprouts can be promoted.

[0016] Ascorbic acid, the synthesis of which is promoted by the sprout cultivation method of the present disclosure, refers to reduced ascorbic acid.

[0017] Furthermore, according to one aspect of the present disclosure, by passing through at least one of the above ultraviolet irradiation step and ultrasonic treatment step, the synthesis of other functional components such as polyphenols, GABA (γ-aminobutyric acid), and BABA (β-aminobutyric acid) contained in the sprouts can also be promoted. In addition, these produced substances are also excellent in antioxidant activity (using DPPH radical scavenging activity as an index).

[0018] The sprout cultivation method of the present disclosure irradiates ultraviolet rays at an output of 0.4 mW / cm 2 or more (hereinafter, also simply referred to as the "ultraviolet irradiation step"), and an ultrasonic treatment step of irradiating seeds with ultrasonic waves at a frequency of 30 kHz or less (hereinafter, also simply referred to as the "ultrasonic treatment step"), and from the viewpoint of further promoting the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprouts, it is preferable to include the ultraviolet irradiation step or the ultrasonic treatment step.

[0019] The type of sprouts cultivated by the sprout cultivation method of the present disclosure is not particularly limited, and known sprouts such as cruciferous broccoli sprouts and radish sprouts, and leguminous soybean sprouts and mung bean sprouts can be applied. Among the above, the sprout cultivation method of the present disclosure is particularly excellent for mung bean sprouts.

[0020] <Ultraviolet Irradiation Step> In the ultraviolet irradiation step, ultraviolet rays are irradiated at an output of 0.4 mW / cm 2Irradiate with the above output. The ultraviolet irradiation process can promote the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprout.

[0021] The ultraviolet ray may be any of long-wavelength ultraviolet rays (UV-A region: wavelength 320 nm to 380 nm), medium-wavelength ultraviolet rays (UV-B region: wavelength 280 nm to less than 320 nm), and short-wavelength ultraviolet rays (UV-C region: wavelength less than 280 nm).

[0022] From the viewpoint of further promoting the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprout, the ultraviolet irradiation process is a process of irradiating with ultraviolet rays at a frequency of 0.4 mW / cm 2 or higher, and it is more preferable that the ultraviolet rays are irradiated at a frequency of 0.4 mW / cm 2 to 5.0 mW / cm 2 ; it is even more preferable that the ultraviolet rays are irradiated at a frequency of 3.0 mW / cm 2 to 5.0 mW / cm 2 ; and it is particularly preferable that the ultraviolet rays are irradiated at a frequency of 3.5 mW / cm 2 to 5.0 mW / cm 2

[0023] From the viewpoint of further promoting the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprout, it is preferable to irradiate the ultraviolet rays for 30 minutes to 15 hours, more preferably for 1 hour to 10 hours, and even more preferably for 2 hours to 8 hours. The irradiation of the ultraviolet rays may be either in a mode of intermittent irradiation or continuous irradiation.

[0024] The timing for performing the ultraviolet irradiation step is not particularly limited, and the seeds may be irradiated before germination, or the germinated seeds and / or new shoots may be irradiated. From the viewpoint of more efficiently promoting the synthesis of at least one of the functional components such as ascorbic acid and glutamic acid contained in the sprouts, for example, the ultraviolet irradiation step may be a step of cultivating the sprouts from the seeds and irradiating them with ultraviolet rays after the 6th day of cultivation, or may be a step of irradiating them with ultraviolet rays after the 7th day of cultivation.

[0025] <Ultrasonic treatment step> In the ultrasonic treatment step, the seeds are irradiated with ultrasonic waves at a frequency of 30 kHz or less. By the ultrasonic treatment step, the synthesis of at least one of the functional components such as ascorbic acid and glutamic acid contained in the sprouts can be promoted.

[0026] The seeds conceptually include not only the state of the seeds before germination but also the seeds with radicles and plumules emerging.

[0027] The time of ultrasonic treatment is not particularly limited. From the viewpoint of further promoting the synthesis of at least one of the functional components such as ascorbic acid and glutamic acid contained in the sprouts, it is preferable to irradiate the seeds with ultrasonic waves for 1 minute to 3 hours by water bath, more preferably for 1 minute to 55 minutes, even more preferably for 1 minute to 50 minutes, and particularly preferably for 5 minutes to 40 minutes.

[0028] In the ultrasonic treatment step, the seeds are irradiated with ultrasonic waves at a frequency of 30 kHz or less. From the viewpoint of more efficiently promoting the synthesis of at least one of the functional components such as ascorbic acid and glutamic acid contained in the sprouts, it is preferable to irradiate the seeds with ultrasonic waves by water bath at a frequency of 1 kHz to 30 kHz, more preferably at a frequency of 10 kHz to 30 kHz, and even more preferably at a frequency of 15 kHz to 30 kHz.

[0029] The ultrasonic treatment step is preferably a step of irradiating the seeds with ultrasonic waves at a frequency of 1 kHz to 30 kHz for 5 minutes to 40 minutes, more preferably a step of irradiating the seeds with ultrasonic waves at a frequency of 10 kHz to 30 kHz for 5 minutes to 40 minutes, and even more preferably a step of irradiating the seeds with ultrasonic waves at a frequency of 15 kHz to 30 kHz for 10 minutes to 40 minutes.

[0030] In the ultrasonic treatment step, from the viewpoint of further promoting the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprout, it is preferable to irradiate the seeds with ultrasonic waves by a water bath at an output of 10 W / 3L to 200 W / 3L, and more preferably to irradiate the seeds with ultrasonic waves by a water bath at an output of 30 W / 3L to 100 W / 3L.

[0031] From the viewpoint of further promoting the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprout, the ultrasonic treatment step preferably irradiates the seeds with ultrasonic waves by a water bath at 10°C to 30°C, more preferably irradiates the seeds with ultrasonic waves by a water bath at 20°C to 30°C, and even more preferably irradiates the seeds with ultrasonic waves by a water bath at 27°C ± 1°C.

[0032] <Other steps> The method for cultivating the sprout of the present disclosure may further include other steps other than the ultraviolet irradiation step and the ultrasonic treatment step. Examples of the other steps include the following steps 1) to 5). Note that the following impregnation step may be a mode in which water is changed to an aqueous solution containing a functional component or a precursor substance thereof during watering during cultivation in the ultraviolet irradiation step and the ultrasonic treatment step. 1) A sterilization step of sterilizing the seeds of the sprout before the ultraviolet irradiation step and the ultrasonic treatment step 2) A hot water treatment step of treating the seeds with hot water (for example, hot water at 90°C to 100°C) for 1 second to 30 seconds before or after the ultraviolet irradiation step and the ultrasonic treatment step 3) An impregnation step of impregnating the seeds or the sprout in the growth process with a solution containing a functional component (for example, a functional component such as ascorbic acid, glutamic acid, salicylic acid, chitosan, etc.) or a precursor substance thereof 4) A second ultrasonic treatment step of irradiating ultrasonic waves to the roots of the sprouts during the growth process 5) A third ultrasonic treatment step of further irradiating ultrasonic waves to the sprouts before harvesting

Example

[0033] Hereinafter, the present disclosure will be described more specifically by way of examples. However, the present disclosure is not limited to the following examples as long as it does not exceed the gist thereof.

[0034] ≪Comparative Example 1≫ Seeds of Chinese mung beans produced in Jilin Province, provided by Asahi Products Co., Ltd., were prepared. After sterilizing the seeds with 200 ppm of sodium hypochlorite, they were placed in a test tube lined with a 5-mm-thick sponge. Then, the upper part of the test tube was sealed with aluminum foil and cultivated in an incubator at 22°C for 8 days. During the cultivation period, 1 mL of sterilized water was submerged every day.

[0035] ≪Example 1: UV1≫ Seeds of Chinese mung beans produced in Jilin Province, provided by Asahi Products Co., Ltd., were prepared. After sterilizing the seeds with 200 ppm of sodium hypochlorite, they were placed in a test tube lined with a 5-mm-thick sponge. Then, 1 mL of sterilized water was put into the test tube, the upper part of the test tube was sealed with aluminum foil, and it was cultivated in an incubator at 22°C for 8 days. At this time, on the 7th day of cultivation after germination, ultraviolet rays with a wavelength of 280 nm to 320 nm were irradiated to the mung beans at an average output of 4.627 mW / cm 2 for 5 hours (ultraviolet irradiation step). During the cultivation period, 1 mL of sterilized water was submerged once a day.

[0036] ≪Example 2: UV2≫ Except that the average output in the ultraviolet irradiation step was set to 0.470 mW / cm 2 , the same specifications as in Example 1 were used to cultivate mung beans.

[0037] ≪Example 3: UL≫ Seeds of Glycine max from Jilin Province, China, provided by Asahi Bussan Co., Ltd. were prepared. After sterilizing the seeds with 200 ppm of sodium hypochlorite, the seeds were irradiated with ultrasonic waves for 30 minutes by a water bath at an output of 100 W / 3L and a frequency of 38 KHz. Then, the seeds were placed in a test tube lined with a 5-mm-thick sponge. Then, 1 mL of sterilized water was put into the test tube, the top of the test tube was sealed with aluminum foil, and the seeds were cultivated in an incubator at 22 °C for 8 days. During the cultivation period, 1 mL of sterilized water was submerged once a day.

[0038] <<Example 4>> Seeds of Glycine max from Jilin Province, China, provided by Asahi Bussan Co., Ltd. were prepared. After sterilizing the seeds with 200 ppm of sodium hypochlorite, the seeds were irradiated with ultrasonic waves for 30 minutes by a water bath at an output of 100 W / 3L and a frequency of 28 KHz. Then, the seeds were placed in a test tube lined with a 5-mm-thick sponge. Then, 1 mL of sterilized water was put into the test tube, the top of the test tube was sealed with aluminum foil, and the seeds were cultivated in an incubator at 22 °C for 8 days. During the cultivation period, 1 mL of sterilized water was submerged once a day.

[0039] <<Comparative Examples 2 to 6>> Seeds of Glycine max from Jilin Province, China, provided by Asahi Bussan Co., Ltd. were prepared. After sterilizing the seeds with 200 ppm of sodium hypochlorite, the seeds were irradiated with ultrasonic waves for 30 minutes at an output of 100 W / 3L and frequencies of 33 KHz, 38 KHz, 40 KHz, 45 KHz, or 100 KHz. Then, the seeds were placed in a test tube lined with a 5-mm-thick sponge. Then, 1 mL of sterilized water was put into the test tube, the top of the test tube was sealed with aluminum foil, and the seeds were cultivated in an incubator at 22 °C for 8 days respectively. During the cultivation period, 1 mL of sterilized water was submerged once a day.

[0040] <<Example 5: UVSS, Example 6: UVSM, Example 7: UVSL>> Seeds of Chinese Luffa cylindrica from Asahi Bussan Co., Ltd. were prepared. The seeds were sterilized with 200 ppm sodium hypochlorite. Then, the seeds were placed in a test tube lined with a 5-mm-thick sponge. Then, 1 mL of sterilized water was placed in the test tube, the top of the test tube was sealed with aluminum foil, and each was cultivated in an incubator at 22 °C for 8 days. At this time, on the 7th day of cultivation, the Luffa cylindrica was irradiated with ultraviolet rays at an average output of 4.627 mW / cm 2 for 2.5 hours, 5 hours, or 7.5 hours (ultraviolet irradiation step). During the cultivation period, 1 mL of sterilized water was submerged once a day.

[0041] ≪Extraction of Functional Components≫ After 8 days of cultivation, the Luffa cylindrica of each example was harvested, and the sample with the roots removed was cut into pieces about 5 mm and placed in a 5-mL mailing tube. After measuring the weight of the tube containing the sample with an electronic balance, it was rapidly frozen with liquid nitrogen and dried with a freeze dryer for 48 hours or more. After drying, the mass of the tube was measured again to obtain the dry mass in the tube. Beads were put into the tube and pulverized at 2500 rpm for 30 seconds using a vibration mill μT01 (Taitec Co., Ltd.) to make a powder. 4 mL of 80% methanol was added to a 15-mL tube containing 0.1 g of the dry powder, then stirred with a vortex mixer and shaken on a table shaker for 36 hours or more. Then, this was taken out and put into a vortex mixer again, and centrifuged under the conditions of 10000 rpm and 1210 minutes. After centrifugation, only the supernatant was collected in a 10-mL volumetric flask, and 3 mL of 80% methanol was added to the residue again. Then, after stirring this with a vortex mixer, it was sonicated for 15 minutes and centrifuged under the same conditions again, and only the supernatant was collected. The same operation was repeated once more, and the collected supernatants were combined and made up to 10 mL with 80% methanol. The obtained 10-mL extract was used for the evaluation of the polyphenol content and the evaluation of the antioxidant activity. For the extraction of ascorbic acid, 5% metaphosphoric acid was used instead of the above-mentioned 80% methanol. 50 mg of dry powder was weighed into a 1.5 mL Eppendorf tube, and 500 μL of 5% metaphosphoric acid was added. After stirring this with a vortex mixer, it was shaken on a table shaker for 1 hour and centrifuged under the conditions of 15,000 rpm for 4 minutes. Only the obtained supernatant was collected, and this supernatant was used for the evaluation of the ascorbic acid content.

[0042] The evaluation was carried out by the following method. The evaluation results are shown in FIGS. 1 to 7. In FIGS. 1 to 4, Comparative Example 1 is denoted as "C", Example 1 as "UV1", Example 2 as "UV2", and Example 3 as "UL", respectively. Also, in FIG. 7, Example 5 with an irradiation time of 2.5 hours is denoted as "UVSS", Example 6 with an irradiation time of 5.0 hours as "UVSM", and Example 7 with an irradiation time of 7.5 hours as "UVSL", respectively.

[0043] ≪Evaluation of ascorbic acid content≫ Using the extract of each example prepared above, the ascorbic acid content was measured with a reflection photometer (RQflex10, manufactured by Merck) and a dedicated test paper (manufactured by Merck).

[0044] FIG. 1 is a graph regarding the content of reduced ascorbic acid in the examples and comparative examples. In the figure, the treatment sections with different small English letters indicate that there is a significant difference at the 5% level as a result of the Tukey test. In the figure, the error bars indicate the standard error (n = 3). As shown in the figure, the cultivation method of the examples had an increased content of reduced ascorbic acid compared to the cultivation method of the comparative examples.

[0045] ≪Evaluation of glutamic acid content≫ Using the extract of each example prepared above, the content of glutamic acid was quantitatively measured by high performance liquid chromatography LC / MS / MS LC-20A 3200 QTRAP (manufactured by Shimadzu Corporation) under the following measurement conditions. <Measurement conditions> Column: Intrada Amino Acid (particle size 3 μm, 150 × 3 mm, Imtakt) Mobile phase: A: 100 mM ammonium formate, B: 0.3% formic acid acetonitrile Ionization method: Electrospray ionization method (ESI), negative charge Measurement: Multiple reaction monitoring (MRM)

[0046] Figure 2 is a graph regarding the content of glutamic acid in the examples and comparative examples. In the figure, the treatment sections with different small English letters indicate that there are significant differences at the 5% level as a result of the Tukey test. In the figure, the error bars indicate the standard error (n = 3). As shown in the illustration, the cultivation method of the examples had an increased content of glutamic acid compared to the cultivation method of the comparative examples.

[0047] ≪Evaluation of polyphenol content≫ The evaluation of the polyphenol content contained in Luffa cylindrica was measured using the Folin-Ciocalteu method. Chlorogenic acid at 100, 200, 300, 400, 600, and 800 ppm was used as the standard for the calibration curve, and 80% methanol was used for 0 ppm. The methanol extract of the sample was inverted and mixed before measurement, centrifuged at 10000 rpm, 12 °C for 3 minutes, and only the supernatant was used for testing. 100 μL each of 80% methanol, chlorogenic acid, and the extract were placed in a 1.5 mL Eppendorf tube. 600 μL of 0.1 M Tris-HCl buffer was added thereto. Further, 100 μL of 50% Folin-Ciocalteu reagent was added, stirred with a vortex mixer, and then allowed to stand for 3 minutes. Thereafter, 200 μL of 2.5% sodium carbonate aqueous solution was added, stirred again, covered from light, and allowed to stand for 60 minutes. 200 μL of the mixed solution was dispensed into a microplate, and the absorbance at 750 nm was measured with a microplate reader (FlexStation3, Molecular Devices). The obtained absorbance value was expressed as chlorogenic acid equivalent by the calibration curve method.

[0048] Figure 3 is a graph showing the polyphenol content in the examples and comparative examples. In the figure, the treatment sections with different small English letters indicate significant differences at the 5% level as a result of the Tukey test. In the figure, the error bars indicate the standard error (n = 3). As shown in the figure, the cultivation method of the examples had an increased polyphenol content compared to the cultivation method of the comparative examples.

[0049] ≪Evaluation of antioxidant activity≫ The evaluation of antioxidant activity was performed using the DPPH radical scavenging activity method. DPPH refers to diphenylpicrylhydrazyl. Trolox at 5, 10, 20, 30, 40, and 50 ppm was used as the standard for the calibration curve, and 80% methanol was used for 0 ppm. The extract diluted 2 - 3 times with 80% methanol was used for measurement. 150 μL each of 80% methanol, Trolox, and the extract were weighed into a dispensing plate and mixed. 20 μL each of this mixed solution was dispensed into a measurement plate, and 180 μL each of a mixed solution of DPPH ethanol and 0.1 M Tris - hydrochloric acid buffer mixed at a ratio of 5:4 was added. After stirring for more than ten seconds, it was left to stand in the dark for 30 minutes. Then, the absorbance at 517 nm was measured using a microplate reader (FlexStation3, Molecular Devices). The results were expressed in Trolox equivalents by the calibration curve method, and this was used as the index value indicating the degree of antioxidant activity.

[0050] Figure 4 is a graph showing the antioxidant activity in the examples and comparative examples. In the figure, the treatment sections with different small English letters indicate significant differences at the 5% level as a result of the Tukey test. In the figure, the error bars indicate the standard error (n = 3). As shown in the figure, it was found that the cultivation method of the examples with the ultrasonic treatment step was superior in antioxidant activity compared to the cultivation method of the comparative examples.

[0051] ≪Evaluation of the output of ultrasonic treatment and the promotion of the synthesis of functional components≫ For each mung bean obtained in Example 4, in which the frequency of the ultrasonic wave applied to the seeds was varied, and Comparative Examples 1 to 6, the contents of glutamic acid and GABA were measured in the same manner as the above-described method. · Glutamic acid Figure 5 is a graph showing the content of glutamic acid in Example 4 and Comparative Examples 1 to 6. In Figure 5, treatment sections with different small English letters indicate a significant difference at the 5% level as a result of the Tukey test. In the figure, error bars indicate the standard error (n = 3). As shown in the figure, it was found that the example with a frequency of 28 kHz in the ultrasonic treatment step was superior in the content of glutamic acid compared to the example with a frequency exceeding 28 kHz in the ultrasonic treatment step. · GABA Figure 6 is a graph showing the content of GABA in Example 4 and Comparative Examples 1 to 6. In Figure 6, treatment sections with different small English letters indicate a significant difference at the 5% level as a result of the Tukey test. In the figure, error bars indicate the standard error (n = 3). As shown in the figure, it was found that the example with an output of 28 kHz in the ultrasonic treatment step had an increased content of GABA compared to the example with an output exceeding 28 kHz in the ultrasonic treatment step.

[0052] ≪Evaluation of Promoting Effect of UV Irradiation Output and Irradiation Time on Synthesis of Functional Components≫ For each mung bean obtained in Examples 5 to 7, in which the irradiation output and irradiation time of ultraviolet rays were different, the contents of glutamic acid and GABA were measured in the same manner as the above-described method. · GABA Figure 7 is a graph showing the content of GABA in Examples 5 to 7 and Comparative Example 1. In the figure, treatment sections with different small English letters indicate a significant difference at the 5% level as a result of the Tukey test. In the figure, error bars indicate the standard error (n = 3). As shown in the figure, it was found that the content of GABA increased as the irradiation time in the ultraviolet irradiation step increased.

[0053] As described above, it was found that the cultivation method of the example can promote the synthesis of at least one functional component of ascorbic acid and glutamic acid contained in the sprout, as compared with the cultivation method of the comparative example.

Claims

1. An ultraviolet irradiation step of irradiating a plant body with ultraviolet rays at an output of 0.4 mW / cm or more, and at least one of an ultrasonic treatment step of irradiating seeds with ultrasonic waves at a frequency of 30 kHz or less. A method for cultivating sprouts. ２

2. The ultrasonic treatment step is a step of irradiating seeds with ultrasonic waves at a frequency of 1 kHz to 30 kHz for 5 minutes to 40 minutes. The method for cultivating sprouts according to Claim 1.

3. The ultrasonic treatment step is a step of irradiating seeds with ultrasonic waves at a frequency of 10 kHz to 30 kHz. The method for cultivating sprouts according to Claim 1 or Claim 2.

4. The ultraviolet irradiation step is a step of irradiating a plant body with ultraviolet rays at an output of 3.0 mW / cm to 5.0 mW / cm. The method for cultivating sprouts according to Claim 1 or Claim 2. ２ ~5.0 mW / cm ２

5. The sprout is mung bean sprouts. The method for cultivating sprouts according to Claim 1 or Claim 2.

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