Means and method for leukocyte subset classification based on multi-parameter cytometry

The reagent composition allows for efficient multiparameter cytometry-based leukocyte subsetting, addressing the limitations of existing methods by simultaneously identifying DCs, monocytes, CD4 T cells, and plasma cells, enhancing immune status monitoring and treatment evaluation.

JP2025114771APending Publication Date: 2025-08-05EUROFORU FOUNDATION +2
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Patent Information

Application Number
JP2025079700
Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Priority Date
2019-11-05
Filing Date
2025-05-12
Publication Date
2025-08-05

AI Technical Summary

Technical Problem

Existing methods are inadequate for efficiently and simultaneously subsetting blood dendritic cells (DCs) in relation to various monocyte subsets and other myeloid cells, CD4 T helper subsets, follicular helper T (Tfh) cells, regulatory T cells (Treg), and distinguishing between mature and functional subsetting within the plasmablast/plasma cell compartment without complex intracellular cytokine profiles.

Method used

A reagent composition comprising antibodies conjugated to detectable labels, targeting specific combinations of markers such as CD141, HLA-DR, CD16, CD33, CD300e, CD303, and CD14 for DC-monocyte subsetting, CD27, CD45RA or CD45RO, CD62L, CD127, CD3, CD25, CCR10, CD183 (CXCR3), CD196 (CCR6), CD194 (CCR4), CD185 (CXCR5), and CD4 for CD4 T cell subsetting, and CD20, CD38, CD62L, CD138 for plasma cell/B cell subsetting, enabling multiparameter cytometry-based leukocyte analysis.

Benefits of technology

Enables simultaneous and efficient subsetting of diverse leukocyte populations, including DCs, monocytes, CD4 T cells, and plasma cells, facilitating comprehensive immune status monitoring and the evaluation of immunomodulatory treatment effects.

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Abstract

To provide an invention in the field of the diagnosis immunology and specifically to provide means and a method for leukocyte subset classification based on a multi-parameter cytometry advantageously used for monitoring an immune state of a subject and / or the effect of immune adjustment processing.SOLUTION: There is provided a reagent composition including an antibody conjugated to a detectable label, and the conjugated antibody is directed to the following combinations of markers: CD141, HLA-DR, CD16, CD33, CD300e, CD303, and CD14. CD300e and CD303 may be conjugated to the same label.SELECTED DRAWING: Figure 3A
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Citation Information

Patent Citations

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