Apparatus for determining the presence of a characteristic in a sample, in particular for determining the sex of fertilized chicken eggs, use and method - Patents.com

JP2025511266A5Pending Publication Date: 2026-04-02テクニッシュ ホーホシューレ オストヴェストファーレン-リッペ
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
Filing Date
2023-03-24
Publication Date
2026-04-02

AI Technical Summary

Technical Problem

Existing methods for in-ovo sex determination in fertilized chicken eggs lack accuracy due to complex fluorescence patterns and unpredictable intensity, necessitating a more precise approach.

Method used

A device utilizing pulsed excitation radiation and time-correlated single-photon counting (TCSPC) to detect intrinsic fluorescence at high temporal resolution, providing two-dimensional data for evaluation, which classifies the data into male and female categories by prioritizing unique wavelengths identified within the time dimension.

Benefits of technology

This method significantly improves the accuracy of sex determination by leveraging high temporal resolution and identifying intrinsic wavelengths that enhance classification accuracy, allowing for precise differentiation between male and female fertilized eggs without the need for hatching.

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Abstract

The invention relates to an apparatus (10) for determining the presence of a characteristic in a sample (12), comprising: a light source (14) emitting pulsed excitation radiation (16); a detection device (18) for detecting the characteristic fluorescence radiation (20) emitted by the sample (12); and a computer-based evaluation means (22), the detection device (18) configured for detecting the characteristic fluorescence radiation (20) of the sample (12) with time resolution by time-correlated single photon counting at different wavelengths and providing the evaluation means (22) with two-dimensional data comprising a wavelength dimension and a time dimension, the evaluation means (22) configured for classifying the provided data into classes by a classifier, the evaluation means (22) configured for identifying characteristic wavelengths to be prioritized during classification based on features formed in the time dimension of the data, the evaluation means (22) configured for preferentially considering data at the characteristic wavelengths for determining the presence of the characteristic in the sample (12). The invention further relates to a method for in-ovo sexing of fertilized chicken eggs (12). The invention further relates to the use of the above-mentioned apparatus (10).
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