Methods and systems for isolating and profiling oncosomes for the early detection and monitoring of all human cancers from peripheral blood

Abstract

A rapid and non-invasive method for isolating oncosomes, extracellular vesicles produced by cancer cells, from human plasma is disclosed. Additionally, a multiplex fluorescent DNA labeling scheme for profiling oncosomes for detecting aneuploidy is revealed. Finally, a very rapid in situ hybridization protocol with a special buffer is disclosed. By combining efficient isolation of oncosomes from plasma with rapid hybridization of fluorescent DNA probes with a multiplex labeling scheme, it is made possible to detect the presence of any human cancer from a liquid biopsy using the hallmark aneuploidy in human cancers.

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