RNA degradation analysis method

JP2025520907A5Pending Publication Date: 2026-07-01SINTEF TTO AS
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
SINTEF TTO AS
Filing Date
2023-06-30
Publication Date
2026-07-01

AI Technical Summary

Technical Problem

Existing methods for analyzing RNA degradation, such as capillary gel electrophoresis, are not sensitive enough to accurately measure the degradation of large RNA molecules, particularly when they are hydrolyzed into small fragments, and do not account for background noise from exoribonuclease activity on intact RNA ends.

Method used

A method using a single-strand specific exoribonuclease in combination with a probe that binds to a target region of ssRNA, preventing exoribonuclease degradation of intact RNA and allowing detection of nucleotides and nucleosides generated from degraded regions, thereby enhancing sensitivity.

Benefits of technology

The method can detect low levels of RNA degradation as low as 0.1% and provides a more accurate measurement of RNA integrity by reducing background noise, making it more sensitive than traditional methods.

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Abstract

The present invention generally relates to methods for analyzing ribonucleic acid (RNA). In particular, the present invention relates to an RNA analysis method for evaluating the degradation level of RNA molecules in an RNA sample. The method uses an exonuclease and a means for detecting nucleotides and / or nucleosides. The present invention provides a method for analyzing ssRNA in a sample, the method comprising: (a) contacting the sample with a probe, wherein the probe binds to a target region of the ssRNA in the sample; (b) incubating the sample with a single-strand specific exoribonuclease; and (c) detecting the nucleotides and / or nucleosides generated by step (b), wherein the nucleotides and / or nucleosides are derived from one or more RNA regions located outside the target region of the ssRNA in the sample. The present invention also provides a kit for carrying out the above method.
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