An in-solution positional covercoding method for sequencing long DNA molecules

Abstract

The method disclosed herein relates to the preparation of libraries for sequencing entire long molecules using massively parallel short-read sequencing. The method disclosed herein generates a nested set of nucleic acid constructs for each genome fragment, and generates multiple nested sets for multiple genome fragments. The nucleic acid constructs can be single-stranded or double-stranded. Each nucleic acid construct in each nested set includes a barcode and a target sequence portion, and the nucleic acid constructs in each nested set have different lengths. The nucleic acid constructs in each nested set share a unique barcode sequence.

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