Compositions and methods of use targeting apoptosis-associated speck-like proteins with a caspase activation and recruitment domain (ASC)

Abstract

The immunogen comprises an immunogenic carrier and an antigenic apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) peptide linked to the immunogenic carrier. In one or more embodiments, the immunogenic carrier is a Qβ virus-like particle (VLP). The immunogen can be formulated into a composition useful for treating inflammatory conditions.

Description

[Technical Field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Patent Application No. 63 / 402,601, filed August 31, 2022, which is incorporated herein by reference in its entirety.

[0002] Government financial support This invention was made with government support under NS083704 awarded by the National Institutes of Health. The government has certain rights in this invention.

[0003] Sequence Listing This application contains a Sequence Listing that has been submitted electronically to the U.S. Patent and Trademark Office via the Patent Center as an .xml file entitled "0310000175WO01.xml" and 21 kilobytes in size, created on August 30, 2023. The information contained in the Sequence Listing is incorporated herein by reference. Summary of the Invention

[0004] In one aspect, the present disclosure describes an immunogen comprising an immunogenic carrier and an antigenic apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) peptide linked to the immunogenic carrier. In one or more embodiments, the immunogenic carrier is a Qβ virus-like particle (VLP).

[0005] In one or more embodiments, the ASC peptide comprises the amino acid sequence of SEQ ID NO: 1, or an antigenic fragment thereof. In one or more of these embodiments, the antigenic fragment of SEQ ID NO: 1 comprises SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, or SEQ ID NO: 12.

[0006] In one or more embodiments, the immunogenic carrier is linked to the ASC peptide via a succinimidyl-6-[β-maleimidopropionamido]hexanoate (SMPH) cross-linker molecule.

[0007] In one or more embodiments, the immunogen further comprises a second antigenic ASC peptide. In one or more of these embodiments, both ASC peptides are displayed on the same VLP.

[0008] In another aspect, the disclosure describes a composition comprising an immunogen comprising an immunogenic carrier and an ASC peptide linked to the immunogenic carrier.

[0009] In one or more embodiments, the composition may comprise a first population of VLPs that display a first antigenic ASC peptide and a second population of VLPs that display a second antigenic ASC peptide.

[0010] In one or more embodiments, the composition may further comprise an adjuvant.

[0011] In one or more embodiments, the composition is a vaccine.

[0012] In another aspect, the disclosure describes a method of treating an inflammatory condition in a subject. Generally, the method includes administering to the subject a therapeutically effective amount of a composition comprising an immunogen comprising an immunogenic carrier and an antigenic ASC peptide linked to the immunogenic carrier.

[0013] In one or more embodiments, the method further comprises administering to the individual at least one additional therapeutic agent for treating the inflammatory condition. In one or more of these embodiments, the immunogen comprises a second antigenic ASC peptide. In one or more of these embodiments, both antigenic ASC peptides are linked to a single carrier.

[0014] In one or more embodiments, the composition comprises a first population of immunogens and a second population of immunogens, the first population of immunogens comprising a first population of immunogenic carriers and a first antigenic ASC peptide linked to the first population of immunogenic carriers, and the second population of immunogens comprising a second population of immunogenic carriers and a second ASC peptide linked to the second population of immunogenic carriers.

[0015] In one or more embodiments, at least one antigenic ASC peptide comprises the amino acid sequence of SEQ ID NO: 1, or an antigenic fragment thereof. In one or more of these embodiments, the antigenic fragment of SEQ ID NO: 1 comprises SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, or SEQ ID NO: 12.

[0016] In one or more embodiments, the composition is administered to the individual before the individual exhibits symptoms or clinical signs of the inflammatory condition.

[0017] In another aspect, the disclosure describes a nucleic acid encoding an immunogen comprising an immunogenic carrier and an antigenic ASC peptide linked to the immunogenic carrier.

[0018] In another aspect, the disclosure describes an expression vector comprising a nucleic acid encoding an immunogen comprising an immunogenic carrier and an antigenic ASC peptide linked to the immunogenic carrier.

[0019] In another aspect, the disclosure describes a host cell comprising an expression vector comprising a nucleic acid encoding an immunogen comprising an immunogenic carrier and an antigenic ASC peptide linked to the immunogenic carrier.

[0020] The above summary is not intended to describe each disclosed embodiment or every implementation of the present invention. The following description more particularly exemplifies exemplary embodiments. In several places throughout the application, guidance is provided through lists of examples, which examples can be used in various combinations. In each instance, the recited list serves only as a representative group and should not be interpreted as an exclusive list. [Brief explanation of the drawings]

[0021] [Figure 1]Structure of the apoptosis-associated speck-like protein containing the caspase activation and recruitment domain (ASC). ASC contains three domains: a pyrin domain (pyr), a flexible semi-structured linker region (linker), and a caspase activation and recruitment domain (CARD). [Figure 2] Peptide sequences of ASC epitopes targeted for VLP generation. Sequences are shown from the amino (N) to carboxy (C) terminus. The location of the ASC epitopes is displayed relative to the overall ASC protein structure. H1-6 represent individual alpha helices within the PYD and CARD regions of the ASC protein. The N-terminal cysteine ​​residue shown in the ASC linker peptide sequence (SEQ ID NO: 3) is an artifact of the conjugation method, in which a terminal cysteine ​​residue is required for the conjugation reaction to occur. Other ASC epitopes contained terminal cysteine ​​residues native to the protein. The remainder of the amino acid sequence shown in the immunogenic portion of SEQ ID NO: 1 (amino acids 92-106 of SEQ ID NO: 1). The amino acid sequences of the ASC helix 4 (SEQ ID NO: 4) immunogenic ASC peptide and the ASC C-terminus (SEQ ID NO: 5) immunogenic ASC peptide are shown. [Figure 3] The Qβ bacteriophage coat protein spontaneously self-assembles into virus-like particles (VLPs). Succinimidyl-6-[β-maleimidopropionamido]hexanoate (SMPH) is a bifunctional cross-linker that chemically conjugates the ASC peptide to surface-exposed lysine residues on Qβ monomers. The final product is a Qβ VLP that displays the ASC peptide in a highly repetitive, multivalent manner. [Figure 4] Mobility shift gel electrophoresis of Qβ and Qβ conjugated to the ASC linker, helix 4, and C-terminal peptide, showing the success of the conjugation reaction for VLP synthesis. [Figure 5]Schematic diagram of the experimental timeline for all preliminary data. Animals were vaccinated at 2 months of age with either the Qβ control or Qβ-ASC VLP vaccine in two doses, 3 weeks apart. Three weeks after the second vaccination, blood samples were collected for antibody titer analysis and used for antibody reactivity experiments in human and mouse brain tissue. Safety monitoring was carried out for an additional 2.5 months. At 6 months of age, endpoint blood samples were collected for blood counts and blood biochemistry profiling. Animals were then injected with 5 mg / kg lipopolysaccharide to induce a systemic inflammatory response. Nine hours later, animals were sacrificed, and serum and brain tissue were collected for inflammasome activation profiling. [Figure 6] Dilution curve of immune serum from mice vaccinated with Qβ ASC-linker construct, showing the generation of an antibody response. [Figure 7] Dilution curve of immune serum from mice vaccinated with the Qβ ASC-helix 4 construct, showing the generation of an antibody response. [Figure 8] Dilution curve of immune serum from mice vaccinated with Qβ ASC-C-terminal construct, showing the generation of antibody responses. [Figure 9] Total body weights of Qβ control and Qβ-ASC VLP vaccinated animals over 4 months. There are no differences in animal weights between treatment groups and controls at any time point indicating that Qβ-ASC VLP vaccination is well tolerated. [Figure 10]Complete blood counts and blood chemistry panels of Qβ-ASC VLP-vaccinated mice compared with Qβ-control at 4 months post-vaccination. Bordered boxes indicate statistically significant differences from Qβ-control (p<0.05, one-way ANOVA). Individual graphs show all statistically significant findings, with dotted lines indicating normal reference ranges. Overall, the Qβ-ASC VLP vaccine was well tolerated. There were no differences in immune cell counts / percentages between treatment and Qβ-control groups. The Qβ-ASC linker vaccine was associated with mild anemia, but no differences in other red blood cell parameters between Qβ-ASC VLP-vaccinated and control groups. There were no differences in platelet characteristics between Qβ-ASC VLP-vaccinated and control groups. There were no changes in multiple markers of organ function assessed by a basic blood chemistry panel. Mice vaccinated with the Qβ-control developed hyperglycemia indicative of diabetes, whereas mice vaccinated with Qβ-ASC VLP maintained normal blood glucose levels. [Figure 11] Immunohistochemical micrographs of human hippocampal brain tissue from an Alzheimer's disease patient stained with a 1:500 dilution of anti-ASC antibody (AdipoGen AL177) or immune serum from Qβ-control or Qβ-ASC VLP-vaccinated animals. Qβ-ASC VLP-induced antibodies bind to human ASC protein in human Alzheimer's disease brain tissue and show strong staining in a pattern similar to that of the commercially available anti-ASC antibody. Qβ control immune serum shows only low levels of nonspecific background staining. [Figure 12]Western blots of mouse and human hippocampal lysates stained with anti-ASC antibody (AdipoGen AL177, 1:2000 dilution) or immune serum from Qβ-control or Qβ-ASC VLP-vaccinated animals (1:1000 dilution). Qβ-ASC VLP vaccination elicits highly specific antibody responses with less off-target binding than commercially available antibodies. Antibodies elicited with the Qβ-ASC helix 4 vaccine showed no binding to denatured protein, suggesting that the antibodies may be specific for the native protein conformation. Qβ-control vaccinated immune serum showed substantial nonspecific antibody binding at molecular weights that overlapped with the nonspecific binding of the commercially available AL177 antibody. ASC-VLP-induced antibodies appear to preferentially bind human proteins over mouse proteins. [Figure 13] Indirect sandwich ELISA of human and mouse hippocampal lysates was performed using an anti-ASC antibody (AdipoGen AL177, 1:100 dilution) as the capture antibody and Qβ-control or Qβ-ASC VLP immune serum (1:1000 dilution) as the detection antibody. The presence of bound vaccine-induced antibodies was detected using an HRP-conjugated goat anti-mouse antibody with 3,3',5,5'-tetramethylbenzidine chromogenic substrate detected at 450 nm. Qβ-ASC VLP-induced antibodies preferentially bound human ASC protein over mouse ASC protein, indicating a highly specific antibody response with limited protein cross-reactivity. The high detection of Qβ-control antibody may be due to overlapping nonspecific protein binding with the AdipoGen AL177 antibody used as the protein capture antibody (see Figure 12). ****p<0.001, **p<0.01, *p<0.05. One-way ANOVA. [Figure 14]ELISA-based quantification of serum interleukin-1β (IL-1β) levels in Qβ control and Qβ-ASC VLP-vaccinated mice injected with lipopolysaccharide (LPS) to induce an inflammasome response. Despite the low affinity of ASC VLP-induced antibodies for mouse ASC protein, there was a trend toward reduced levels of mature IL-1β in the serum of mice vaccinated with Qβ-ASC linker and Qβ-ASC helix 4, suggesting possible neutralization of endogenous mouse ASC protein by circulating Qβ-ASC VLP-induced antibodies. [Figure 15] Western blot of hippocampal brain lysates from Qβ-control and Qβ-ASC VLP-vaccinated mice after stimulation with lipopolysaccharide (LPS) to induce inflammasome activation. Caspase-1, a downstream effector protein of the inflammasome, is significantly reduced in all Qβ-ASC VLP-treated groups compared to the Qβ-control group. **p<0.01, ***p<0.005. One-way ANOVA. [Figure 16] The microglial NLRP3 inflammasome is primed and activated by AD protein aggregates. Schematic of the cellular pathways involved in inflammasome activation. [Figure 17] Diagram of the ASC-dependent mechanism by which inflammasomes propagate inflammation and the spread of misfolded protein aggregation. DETAILED DESCRIPTION OF THE INVENTION

[0022] This disclosure describes compositions and methods that target apoptosis-associated speck-like proteins containing a caspase activation and recruitment domain (ASC), which is also sometimes referred to in the scientific literature by the name of the gene that encodes the protein, PYCARD. The compositions can be used to treat inflammatory conditions by targeting the inflammasome.

[0023] Inflammasomes are natural immune complexes capable of sensing a variety of pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs). The canonical inflammasome initiates an inflammatory signaling cascade mediated by the maturation of interleukin-1β and interleukin-18 by caspase-1. Inflammasomes are involved in many disease states and often contribute to the prolonged cytotoxic inflammatory state in many diseases characterized by chronic inflammation, including Alzheimer's disease. While several different inflammasome complexes exist that respond to different PAMPs and DAMPs, apoptosis-associated speck-like proteins containing a caspase activation and recruitment domain (ASC) are a common component of most inflammasomes. They are components of the ASC-speck, a large protein complex that functions as a signal amplification platform to enhance inflammasome activity.

[0024] Alzheimer's disease (AD) is one of the leading causes of death worldwide. Currently, no treatments have been shown to prevent or cure AD. Four pathological hallmarks of AD in the brain include the accumulation of extracellular amyloid-β protein aggregates, intracellular accumulation of tau protein aggregates, low-level chronic neuroinflammation, and widespread neurodegeneration leading to memory loss and cognitive impairment. Therapeutic approaches have largely ignored the role of chronic neuroinflammation in disease progression. The NLRP3 inflammasome / ASC-speck signaling complex, derived from inflammatory microglia, is a mediator of the chronic inflammatory state in the AD brain. Genetic knockout of either the NLRP3 or ASC gene attenuates AD disease pathology in animal models.

[0025] This disclosure describes novel vaccine compositions against the NLRP3 inflammasome / ASC-speck signaling complex. The compositions described herein generate neutralizing antibody responses that reduce neuroinflammation and inhibit disease progression in AD. These compositions include a virus-like particle (VLP) vaccine platform (e.g., a Qβ-based VLP platform) for antigenic display of short peptide sequences from apoptosis-associated speck-like proteins containing the caspase activation and recruitment domain (ASC) protein, a component of the NLRP3 inflammasome / ASC-speck.

[0026] The amino acid sequence of the human ASC protein is reflected in SEQ ID NO: 1. The human ASC protein contains a pyrin domain (amino acids 1-91 of SEQ ID NO: 1) and a caspase activation and recruitment domain (CARD) domain (amino acids 107-195 of SEQ ID NO: 1). The amino acid sequence of the mouse ASC protein is reflected in SEQ ID NO: 2. The mouse ASC protein contains a pyrin domain (amino acids 1-91 of SEQ ID NO: 2) and a caspase activation and recruitment domain (CARD) domain (amino acids 105-193 of SEQ ID NO: 2). The human ASC protein exists in at least three isoforms. Isoform 1 is shown in SEQ ID NO: 1 and is the canonical form with 195 amino acid residues. Isoform 2 has 176 amino acid residues: amino acids 1-92 and 112-195 of SEQ ID NO: 1, i.e., SEQ ID NO: 1 with amino acids 93-111 truncated. Isoform 3 has 135 amino acid residues: amino acids 1-25 and 86-195, i.e., SEQ ID NO: 1 with amino acids 26-85 truncated. Isoform 1 and isoform 2 are considered active isoforms of ASC. Isoform 3 binds to caspase-1 and has some inhibitory function.

[0027] Each vaccine contained an ASC peptide conjugated to a Qβ bacteriophage virus-like particle (VLP). In one vaccine, referred to herein as the "ASC linker" vaccine, the ASC peptide comprises amino acids 92-106 of SEQ ID NO: 1 and targets the linker region between the pyrin and CARD domains (Figure 2). In a second vaccine, referred to herein as the "ASC helix 4" vaccine, the ASC peptide comprises amino acids 155-173 of SEQ ID NO: 1 and targets helix 4 of the CARD domain. In a third vaccine, referred to herein as the "ASC C-terminus" vaccine, the ASC peptide comprises amino acids 173-195 and targets the C-terminus of the CARD domain.

[0028] For safety studies, animals received a two-injection vaccine regimen starting at 2 months of age, with a booster vaccination 3 weeks after the first injection. Vaccine conditions included three different ASC peptide sequences conjugated to Qβ bacteriophage VLPs or unconjugated Qβ VLP sham treatment, for a total of four treatment groups (5 mice per group). Each ASC-VLP group demonstrated significantly higher increases in IgG titers against the ASC peptide compared with sham-vaccinated mice. There were no differences in survival or animal weight between the ASC-vaccinated and sham-vaccinated groups up to 4 months post-vaccination. Complete blood counts and blood chemistry panels were also performed on each mouse and showed no significant differences indicating increased severity of disease or infection. These findings indicate that all three vaccines can be safely used in mouse models of various diseases, including Alzheimer's disease, for therapeutic intervention of inflammasome-mediated cytokine signaling in preclinical studies.

[0029] Excessive interleukin-1 signaling contributes to inflammatory diseases Interleukin-1 (IL-1) is a proinflammatory cytokine that plays diverse roles in health and disease as a key signaling component of the innate immune system. It is a master regulator of inflammation through several innate immune processes, and its functions include leukocyte pyrogen, leukocyte intrinsic mediator and activator, activator of the acute phase response to infection and injury, and mediator of fever. IL-1 plays a protective role in the acute response to infection and tissue injury, but dysregulation of IL-1 signaling can contribute to a variety of pathologies, including autoinflammatory diseases (e.g., cryopyrin-associated periodic syndrome, familial Mediterranean fever, etc.), metabolic syndromes (e.g., type 2 diabetes), excessive acute inflammation (e.g., sepsis), chronic inflammatory diseases (e.g., rheumatoid arthritis, chronic obstructive pulmonary disease, gout, Alzheimer's disease, etc.), and malignancies (e.g., HER2-negative breast cancer). The U.S. Food and Drug Administration has approved several drugs that directly target IL-1β signaling for the treatment of a wide range of inflammatory diseases. Although directly targeting IL-1 signaling can be effective in managing some diseases, targeting upstream IL-1 production may be more effective.

[0030] Inflammasomes are mediators of IL-1 signaling Inflammasomes are natural immune signaling complexes that catalyze the maturation of IL-1 cytokines for inflammatory signaling. The NLRP3 inflammasome is a multiprotein oligomeric complex containing the Nod-like receptor family pyrin domain-containing 3 (NLRP3) protein, the apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC) protein, and caspase-1 (Figure 16). The ASC protein is a common component of multiple inflammasome types, including the NLRP1, NLRP3, NLRP6, NLRP12, NLRC4, and AIM2 inflammasomes. Upon inflammasome activation, ASC can polymerize into large fibrils, which can condense together into large supramolecular complexes called ASC specks. ASC specks are protein complexes approximately 1 micron in diameter that function as signal amplification platforms to enhance IL-1 maturation.

[0031] NLRP3 inflammasome-mediated interleukin-1β signaling is associated with and may drive the pathology of Alzheimer's disease and other neurodegenerative diseases. Reactive microglia, innate immune cells in the brain, promote tau pathology in an IL-1-dependent manner in animal models of Alzheimer's disease. This IL-1 signaling is primarily driven by NLRP3 inflammasome / ASC-speck activity from microglia. Therefore, therapeutics targeting inflammasome / ASC-speck activity within the central nervous system may be beneficial for the treatment of Alzheimer's disease and other neurodegenerative disorders. Conventional immunotherapies for treating Alzheimer's disease include vaccines that target tau protein using a virus-like particle (VLP) platform. These VLPs generate robust antibody responses against pathological tau protein aggregates, clearing these aggregates from the brain and improving memory and cognition in rodent models of Alzheimer's disease. VLPs offer advantages over monoclonal antibody immunotherapy because they are inexpensive, simple to develop, and do not require frequent re-administration throughout an individual's lifetime. This disclosure describes a similar approach using VLP technology applied to inflammasome / ASC specs for the treatment of inflammatory diseases, including Alzheimer's disease.

[0032] VLP Display Many viral structural proteins have the inherent ability to self-assemble into virus-like particles (VLPs), which are structurally similar to the viruses from which they are derived but lack the viral genome, making them completely noninfectious. VLPs not only function as standalone vaccines, but also serve as a platform for enhancing the immunogenicity of heterologous antigenic targets, as their particulate nature and multivalent structure elicit potent immune responses. For example, when short immunogenic peptides are presented on VLPs in a highly repetitive, multivalent manner, peptide-specific B cells are potently activated, resulting in high-titer, long-lasting antibody responses. VLPs derived from diverse virus types can serve as effective platforms for antigen display. The immunogens described herein are based on VLPs derived from a family of related single-stranded RNA bacteriophages, including MS2, PP7, AP205, and Qβ. These VLPs can be produced by expressing a single viral structural protein, called coat, from a plasmid in bacteria. Peptides can be displayed on VLPs through bioconjugation techniques using cross-linker molecules. In one or more embodiments, peptides can be displayed on VLPs by conjugating the peptide to the VLP via a succinimidyl-6-[β-maleimidopropionamido]hexanoate (SMPH) crosslinker molecule. This technique results in VLPs that display target peptides at high valency (typically 180-360 peptides per VLP), conferring potent immunogenicity to the displayed immunogenic peptides.

[0033] VLP-based vaccines against inflammasome-mediated inflammatory diseases This vaccine technology has therapeutic potential for the treatment of various inflammatory diseases, including but not limited to autoinflammatory diseases, metabolic syndrome, acute inflammation, chronic inflammatory diseases, malignancies, and neurodegenerative diseases such as Alzheimer's disease.

[0034] An exemplary embodiment of an ASC-targeted vaccine targets an epitope on the ASC protein (Figure 1). The targeted epitope includes a flexible linker sequence, alpha helix 4 from the CARD region, and the C-terminal region containing alpha helices 5 and 6 from the CARD region (Figure 2). In one or more alternative exemplary embodiments, other epitopes may also be targeted. The Qβ bacteriophage coat protein, which naturally self-assembles into VLPs, was used as an antigen display platform and chemically conjugated to an ASC epitope peptide sequence using succinimidyl-6-[β-maleimidopropionamido]hexanoate (SMPH) as a chemical crosslinker (Figure 3). The final product is a Qβ VLP that displays the ASC peptide epitope in a multivalent, highly repetitive manner, capable of inducing a potent B cell response for the production of high-affinity antibodies against the ASC epitope antigen.

[0035] While Figure 3 shows an exemplary embodiment in which the VLP displays a single ASC peptide in a multivalent manner (i.e., the VLP displays multiple copies of a single ASC peptide), vaccines can be designed in which the VLP displays multiple ASC peptides. Thus, in another embodiment, the VLP may display one, two, three, or more different ASC peptides. The efficiency of peptide conjugation on the VLP can be assessed using any conventional method, such as, for example, mobility shift gel electrophoresis. Figure 4 shows the mobility shift of Qβ to higher molecular weights depending on the number of ASC peptides successfully conjugated to the Qβ monomer.

[0036] Once produced, ASC-VLP compositions can be used to immunize subjects. The immunogenicity of a given ASC-VLP vaccine composition can be assessed using any conventional method, such as, for example, enzyme-linked immunosorbent assay (ELISA). ASC linker, ASC helix 4, and ASC C-terminus VLPs are immunogenic and elicit high-titer IgG antibody responses. Figures 6-8 present data demonstrating the immunogenicity of ASC linker (Figure 6), ASC helix 4 (Figure 7), and ASC C-terminus (Figure 8) VLPs.

[0037] The safety of a given ASC-VLP vaccine composition can be assessed by monitoring immunized subjects (e.g., for 6 months) for adverse health effects. For example, subjects can be monitored using complete blood count profiles, basic blood chemistry panels, weight changes, and survival. Figure 5 provides a schematic of the experimental timeline of all preliminary data. The experimental timeline in Figure 5 shows that safety monitoring was conducted for an additional 2.5 months after the VLP vaccine injection dose and after the first blood draw was collected.

[0038] For example, Figure 9 shows data demonstrating that the ASC linker, ASC helix 4, and ASC C-terminus vaccines were well tolerated, with none of the vaccines causing significant weight loss compared to sham vaccination with Qβ VLP lacking the ASC peptide. Similarly, Figure 10 shows complete blood cell profiles demonstrating that all vaccines were well tolerated, with mild anemia (e.g., low hemoglobin and low hematocrit) occurring only with the ASC linker vaccine. Figure 10 also shows a basic blood chemistry panel, further demonstrating that all vaccines were well tolerated and there were no markers of organ damage or dysfunction.

[0039] The efficacy of ASC-VLP vaccine compositions can be assessed using any suitable measure of inflammasome activity. In the exemplary context of Alzheimer's disease, an exemplary model designed to measure cognitive performance may be used. For example, healthy C57B16 / J mice may be injected into the hippocampus with brain lysate from a human AD patient or PBS vehicle. The animals may then be further divided into groups receiving ASC-VLP vaccination and sham vaccination. After a predetermined period (e.g., 2 months in some exemplary models), the animals may be subjected to a series of cognitive-behavioral tasks and then sacrificed for histological and biochemical analysis of disease pathology and inflammatory markers.

[0040] Metabolic disorders such as type 2 diabetes have been linked to inflammasome activity as a potential risk factor. Figure 10 shows that mice sham-vaccinated with Qβ VLP lacking the ASC peptide developed hyperglycemia indicative of diabetes, whereas all ASC-VLP-vaccinated animals maintained healthy blood glucose levels. This was an exploratory finding that represents another potential exemplary model for measuring the effect of ASC-VLP vaccination on inflammasome-mediated pathologies.

[0041] Thus, the present disclosure describes compositions and methods that offer cutting-edge immunotherapy approaches that target proteins involved in damaging inflammation. The compositions described herein target inflammasome-associated proteins and inflammatory cytokines. In one or more embodiments, the compositions and methods described herein induce antibody production while minimizing the induction of severe inflammatory responses. Therefore, the compositions and methods may be suitable for use in treating inflammatory conditions, including, but not limited to, Alzheimer's disease. A vaccine targeting ASC reduces neuroinflammation and disease pathology in animal models of AD. The NLRP3 inflammasome contributes to the pathological tau aggregation process through modulation of kinase / phosphatase activity by inflammatory signals and can be targeted to reduce ptau burden.

[0042] The compositions include VLP-based immunogens comprising antigenic ASC peptides (also referred to herein as "ASC targeting peptides"), such as, for example, the amino acids of SEQ ID NO: 1, SEQ ID NO: 2, or immunogenic fragments thereof. Exemplary immunogenic ASC peptide fragments include, but are not limited to, any one of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21, or immunogenic fragments of any of the foregoing. Furthermore, immunogens can comprise VLPs presenting multiple populations of antigenic ASC peptides, e.g., a first population of antigenic ASC peptides comprising amino acids of a first ASC immunogenic peptide and a second population of antigenic ASC peptides comprising a second immunogenic ASC peptide. Thus, immunogens can be designed to present one, two, three, four, five, six, or more antigenic ASC peptides.

[0043] In another embodiment, an immunogenic composition can comprise multiple populations of VLPs. For example, an immunogenic composition can comprise a first population of VLPs that display a first antigenic ASC peptide and a second population of VLPs that display a second antigenic ASC peptide. Still further, an immunogenic composition can comprise a first population of VLPs that display one or more antigenic ASC peptides and a second population of VLPs that display one or more antigenic ASC peptides, where the number and identities of antigenic peptides displayed by the VLPs of the second population are independent of the number and identities of antigenic peptides displayed by the first population of VLPs.

[0044] As used herein, an antigenic ASC peptide can refer to any ASC amino acid sequence that elicits an ASC-targeted immune response when introduced into an immunocompetent subject. Exemplary antigenic ASC peptides include peptides comprising any one or more of the amino acid sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, and SEQ ID NO:21, any immunogenic fragment of any of the foregoing, or any peptide structurally similar to any of the foregoing peptides or immunogenic fragments.

[0045] As used herein, a peptide is "structurally similar" to a reference polypeptide if the peptide's amino acid sequence has a certain amount of identity compared to the reference peptide. The structural similarity of two peptides can be confirmed by aligning the residues of the two peptides (e.g., a candidate polypeptide and any one of SEQ ID NOS: 1-21) to optimize the number of identical amino acids along the length of their sequences. Gaps in either or both sequences are allowed when aligning to optimize the number of identical amino acids, but the amino acids in each sequence must still maintain their proper order. A candidate peptide is a peptide that is being compared to a reference peptide (e.g., any one of SEQ ID NOS: 1-21). Candidate peptides can be, for example, isolated from animals, produced using recombinant techniques, or synthesized chemically or enzymatically.

[0046] Pairwise comparison analysis of amino acid sequences can be performed using the BESTFIT algorithm in the GCG package (version 10.2, Madison, WI). Alternatively, peptides can be compared using the BLAST 2 search algorithm, Blastp, described by Tatiana et al. (FEMS Microbiol Lett, 174, 247-250 (1999)) and available on the National Center for Biotechnology Information (NCBI) website. Default values ​​for all BLAST 2 search parameters can be used, including matrix = BLOSUM62, open gap penalty = 11, extended gap penalty = 1, gap x_dropoff = 50, expectation = 10, word size = 3, and filter on.

[0047] An antigenic ASC peptide can contain amino acids in addition to any one of SEQ ID NOs: 1-21, so long as the additional amino acids do not eliminate immunogenicity to ASC. For example, an antigenic ASC peptide can have a linker region containing the amino acids GGGC (SEQ ID NO: 22) or CGGG (SEQ ID NO: 23).

[0048] In comparing two amino acid sequences, structural similarity is sometimes referred to as percent "identity" or percent "similarity." "Identity" refers to the presence of identical amino acids. "Similarity" refers not only to the presence of identical amino acids but also to the presence of conservative substitutions. Conservative substitutions for amino acids in the immunogenic peptides described herein can be selected from other members of the class to which the amino acid belongs. For example, in the art of protein biochemistry, it is well known that an amino acid belonging to a group of amino acids having a particular size or property (such as charge, hydrophobicity, and hydrophilicity) can be substituted with another amino acid without altering the activity of the protein, particularly in regions of the protein not directly related to biological activity. For example, nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and tyrosine. Polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine. Positively charged (basic) amino acids include arginine, lysine, and histidine. Negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Conservative substitutions include, for example, substitution of Arg with Lys and vice versa to maintain a positive charge, substitution of Asp with Glu and vice versa to maintain a negative charge, substitution of Thr with Ser to maintain a free -OH, and substitution of Asn with Gln to maintain a free -NH. Similarly, biologically active analogs of polypeptides containing deletions or additions of one or more consecutive or non-consecutive amino acids that do not eliminate the functional activity of the peptide are also contemplated.

[0049] Exemplary ASC targeting peptides are listed in Table 1.

[0050] [Table 1]

[0051] Thus, in one or more embodiments, the ASC targeting peptides described herein can comprise a peptide (or peptide domain - i.e., a portion of a larger amino acid sequence) with at least 66%, at least 70%, at least 75%, at least 79%, at least 81%, at least 83%, at least 84%, at least 87%, at least 90%, at least 93%, or at least 96% sequence similarity to the amino acid sequence of any one of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, or SEQ ID NO:21. Thus, in one or more embodiments, an ASC targeting polypeptide comprising a domain corresponding to any one of SEQ ID NOs: 3-21, a portion of an ASC peptide corresponding to SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21, can comprise a total of no more than six, no more than five, no more than four, no more than three, no more than two, or no more than one amino acid deletion, amino acid addition, and / or non-conservative amino acid substitution compared to a reference ASC peptide sequence.

[0052] In one or more embodiments, the ASC targeting peptides described herein can comprise a peptide (or peptide domain - i.e., a portion of a larger amino acid sequence) with at least 66%, at least 70%, at least 75%, at least 79%, at least 81%, at least 83%, at least 84%, at least 87%, at least 90%, at least 93%, or at least 96% sequence identity to any one of the amino acids in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, or SEQ ID NO:21. Thus, in one or more embodiments, an ASC targeting polypeptide comprising a domain corresponding to any one of SEQ ID NOs: 3-21, a portion of an ASC peptide corresponding to SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, or SEQ ID NO: 21, can contain a total of no more than 6, no more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 amino acid deletion and / or addition compared to a reference ASC peptide sequence.

[0053] In one or more embodiments, the ASC targeting peptides described herein can be designed to provide additional sequences, such as added C- or N-terminal amino acids, that facilitate purification, for example, by capture on a column or the use of an antibody. Such tags include, for example, histidine-rich tags that allow for purification of the polypeptide on a nickel column. Such genetic modification techniques and appropriate additional sequences are well known in the art of molecular biology. Alternatively or additionally, the additional amino acid sequence can facilitate conjugation or other binding of the ASC targeting peptide to a VLP.

[0054] Virus-like particles (VLPs) can include any particle that contains viral proteins assembled to resemble the virus from which they are derived, but lacks sufficient viral genome to be non-replicative and therefore non-infectious. Thus, VLPs can contain at least a portion of a viral genome, but the viral genome has been genetically modified so that the viral genes responsible for infectivity and / or replication are inactivated. Exemplary VLPs include those derived from Qβ, MS2, PP7, AP205, and other bacteriophage coat proteins, capsid and core proteins of hepatitis B virus, paralysis virus, Sindbis virus, rotavirus, foot-and-mouth disease virus, Norwalk virus, retroviral GAG proteins, retrotransposon Ty protein pl, surface proteins of hepatitis B virus, human papillomavirus, human polyomavirus, RNA phages, Ty, fr phages, GA-phages, AP 205-phages and, in particular, Qβ-phages, cowpea chlorotic mottle virus, cowpea mosaic virus, human papillomavirus (HPV), bovine papillomavirus, porcine parvovirus, parvoviruses such as B19, porcine (PPV) and canine (CPV) parvoviruses, caliciviruses (e.g., Norwalk virus, rabbit hemorrhagic disease virus [RHDV]), animal hepadnavirus core antigen VLPs, tobacco mosaic virus (TMV), potato virus X (PVX), papain virus, and the like. These include, but are not limited to, VLPs of filamentous / rod-shaped plant viruses, including, but not limited to, Papaya mosaic virus (PapMV), Alfalfa mosaic virus (AIMV), and Johnsongrass mosaic virus (JGMV), insect viruses, such as Flock House virus (FHV) and Tetravirus, polyomaviruses, such as mouse polyomavirus (MPyV), mouse pneumotropic virus (MPtV), BK virus (BKV), and JC virus (JCV).

[0055] Antigenic ASC peptide can be linked to immunogenic carrier through chemical conjugation or by expressing genetically engineered fusion partner.Linkage does not necessarily have to be direct, but can be via a linker sequence.More generally, when antigenic peptide is fused to, conjugated with, or otherwise linked to immunogenic carrier, spacer sequence or linker sequence is typically added at one or both ends of antigenic peptide.Such linker sequence generally includes a sequence that is recognized by proteasome, endosomal protease, or other vesicular compartment of cell.

[0056] In one embodiment, the antigenic ASC peptide can be presented as a fusion protein with a subunit of an immunogenic carrier. The peptide fusion can be achieved by inserting the ASC antigenic peptide amino acid sequence into the primary sequence of the immunogenic carrier, or by fusing it to either the N-terminus or C-terminus of the immunogenic carrier.

[0057] When the immunogenic carrier is a VLP, the chimeric antigenic peptide-VLP subunit may be capable of self-assembly into a VLP. The VLP-presented epitope fused to the subunit is also referred to herein as a chimeric VLP. For example, European Application No. EP90310264A (European Patent No. EP0421635 B1) describes the use of chimeric hepadnavirus core antigen particles to present foreign peptide sequences in virus-like particles.

[0058] Flanking amino acid residues can be added to either end of the peptide sequence so that the antigenic ASC targeting peptide is fused to either end of the VLP subunit sequence, or for internal insertion of such a peptide sequence into the VLP subunit sequence. Glycine and serine residues are particularly preferred amino acids for use in the flanking sequence added to the fused peptide. Glycine residues provide additional flexibility, which may reduce the potentially destabilizing effect of fusing a foreign sequence to the VLP subunit sequence.

[0059] In one or more embodiments, the immunogenic carrier is a VLP of an RNA phage, such as Qβ. The major coat protein of RNA phages naturally assembles into VLPs when expressed in bacteria (e.g., E. coli). Fusion protein constructs in which antigenic peptides are fused to the C-terminus of a truncated A1 protein of Qβ or inserted internally into the A1 protein have been described (Kozlovska et al., 1996, Intervirology 39:9-15). Assembly of Qβ particles displaying fused epitopes typically involves the presence of both an A1 protein-antigen fusion and wild-type coat protein to form a mosaic particle. However, embodiments are contemplated that include VLPs, particularly VLPs of RNA phage Qβ coat protein, composed solely of VLP subunits with antigenic peptides fused thereto.

[0060] The generation of mosaic particles can be carried out in several ways. In one exemplary approach, efficient display of the fused epitope on the VLP is mediated by expression of a plasmid encoding a Qβ A1 protein fusion with a UGA stop codon between the coat protein and the coat protein extension in an E. coli strain harboring a plasmid (pISM3001 plasmid) encoding a cloned UGA suppressor tRNA (resulting in translation of the UGA codon to Trp). In a second exemplary approach, the coat protein gene stop codon is modified to UAA and cotransformed with a second plasmid expressing the A1 protein-antigen fusion. The second plasmid encodes a different antibiotic resistance and has a replication origin compatible with that of the first plasmid. In a third exemplary approach, the Qβ coat protein and the A1 protein-antigen fusion are bicistronically encoded and operably linked to a promoter such as the Trp promoter.

[0061] Additional VLPs suitable for the fusion of antigens or antigenic determinants are described, for example, in International Patent Application No. PCT / IB2002 / 004132 (International Publication No. WO 03 / 024481 A2), and include bacteriophage fr, RNA phase MS-2, papillomavirus proteins, retrotransposon Ty, yeast and retrovirus-like particles, HIV2 Gag, cowpea mosaic virus, parvovirus VP2 VLP, and HBsAg (U.S. Pat. No. 4,722,840). Examples of chimeric VLPs suitable for use as immunogenic carriers include those described in Kozlovska et al., 1996, Intervirology 39:9-15. Further examples of VLPs suitable for use as immunogenic carriers include, but are not limited to, HPV-1, HPV-6, HPV-11, HPV-16, HPV-18, HPV-33, HPV-45, CRPV, COPV, HIV GAG, tobacco mosaic virus, SV-40 virus-like particles, polyomavirus, adenovirus, herpes simplex virus, rotavirus, and Norwalk virus.

[0062] In one or more embodiments, vaccine constructs containing an ASC peptide containing the amino acid sequence of SEQ ID NO:3, SEQ ID NO:4, or SEQ ID NO:5 are synthesized by conjugating the peptide to a Qβ bacteriophage VLP using a bifunctional crosslinker (SMPH). The ASC peptide can be modified to include a linker peptide at the C-terminus (e.g., a GGGC linker sequence, SEQ ID NO:6) or N-terminus (e.g., a CGGG linker sequence, SEQ ID NO:7). The SMPH crosslinker conjugates free amines on the surface of the Qβ VLP to cysteine ​​residues of the linker peptide. In one exemplary synthesis methodology, the Qβ VLP is purified from free, unconjugated crosslinker and then reacted with the ASC peptide at a molar ratio of approximately 10 peptide:1 VLP.

[0063] For any recombinantly expressed antigenic ASC peptide described herein (whether or not conjugated to an immunogenic carrier), the present disclosure describes isolated nucleic acid sequences that encode any embodiment of the antigenic ASC targeting peptide, or any component fragment of the antigenic ASC targeting peptide, including the amino acid sequence of any one of SEQ ID NO: 1, SEQ ID NO: 2, or any fragment thereof. In some embodiments, the isolated nucleic acid encodes an antigenic ASC targeting peptide comprising the amino acids of SEQ ID NO: 3, SEQ ID NO: 4, or SEQ ID NO: 5. Given the amino acid sequence of any antigenic ASC targeting peptide, or one or more component fragments of an antigenic ASC targeting peptide, one of skill in the art can determine the full range of polynucleotides encoding that amino acid sequence using conventional methods.

[0064] As used herein, the term "nucleic acid" or "oligonucleotide" refers to a polynucleotide such as deoxyribonucleic acid (DNA) or ribonucleic acid (RNA). Nucleic acids include, but are not limited to, genomic DNA, cDNA, mRNA, iRNA, miRNA, tRNA, ncRNA, rRNA, and recombinantly produced and chemically synthesized molecules, such as aptamers, plasmids, antisense DNA strands, shRNA, ribozymes, nucleic acid conjugates, and oligonucleotides. Nucleic acids can be single-stranded, double-stranded, linear, or covalently closed circular molecules. Nucleic acids can be isolated. The term "isolated nucleic acid" means that the nucleic acid is (i) amplified in vitro, e.g., via polymerase chain reaction (PCR), (ii) recombinantly produced by cloning, (iii) purified, e.g., by cleavage and separation by gel electrophoresis, (iv) synthesized, e.g., by chemical synthesis, or (vi) extracted from a sample. The nucleic acid can be introduced, i.e., transfected, into a cell. When RNA is used to transfect cells, the RNA may be modified by stabilizing modifications, capping, or polyadenylation.

[0065] As used herein, "amplified DNA" or "PCR product" refers to an amplified DNA fragment of a defined size. Various techniques are available and well known in the art for detecting PCR products. Methods for detecting PCR products include gel electrophoresis using agarose or polyacrylamide gels with ethidium bromide staining (a DNA intercalant), labeled probes (radioactive or non-radioactively labeled, Southern blotting), labeled deoxyribonucleotides (direct incorporation of radioactive or non-radioactive labels) or silver staining for direct visualization of amplified PCR products, restriction endonuclease digestion relying on agarose gel electrophoresis, polyacrylamide gel electrophoresis, or high-performance liquid chromatography (HPLC), amplification of specific labeled probes (radioactive or non-radioactively labeled). These include, but are not limited to, dot blots using wide DNA hybridization, high pressure liquid chromatography with ultraviolet detection, electrochemiluminescence coupled with voltage-initiated chemical reactions / photon detection, direct sequencing using radioactive or fluorescently labeled deoxyribonucleotides to determine the exact order of nucleotides in a DNA fragment of interest, oligo ligation assay (OLA), PCR, qPCR, DNA sequencing, fluorescence, gel electrophoresis, magnetic beads, allele-specific primer extension (ASPE) and / or direct hybridization.

[0066] Generally, nucleic acids can be extracted, isolated, amplified, or analyzed by various techniques, such as those described by Green and Sambrook, Molecular Cloning: A Laboratory Manual (Fourth Edition), Cold Spring Harbor Laboratory Press, Woodbury, NY, 2,028 pages (2012), or those described in U.S. Patent Nos. 7,957,913, 7,776,616, 5,234,809, and 9,012,208. Examples of nucleic acid analysis include, but are not limited to, sequencing and DNA-protein interaction. Sequencing can be by any method known in the art. DNA sequencing techniques include classical dideoxy sequencing reactions (Sanger sequencing) using labeled terminators or primers and gel separation in slabs or capillaries, as well as next-generation sequencing methods such as sequencing-by-synthesis using reversibly terminated labeled nucleotides, pyrosequencing, 454 sequencing, Illumina / Solexa sequencing, allele-specific hybridization of labeled oligonucleotide probes to a library, sequencing-by-synthesis using allele-specific hybridization to a library of labeled clones followed by ligation, real-time monitoring of incorporation of labeled nucleotides during the polymerization step, polony sequencing, and SOLiD sequencing. Separated molecules can be sequenced by sequential or single extension reactions using polymerases or ligases, as well as by single or sequential differential hybridization with a library of probes.

[0067] In another aspect, the disclosure describes a host cell comprising any of the isolated nucleic acid sequences and / or proteins described herein. Thus, the disclosure encompasses translation of the nucleic acid (e.g., mRNA) by the host cell to produce an ASC-VLP subunit.

[0068] The nucleic acid constructs of the present invention can be introduced into modified host cells to allow expression of the ASC-VLP subunit peptide within the cells, thereby generating genetically engineered cells. Various methods are known in the art and are suitable for introducing nucleic acids into cells, including viral and non-viral mediated techniques. Exemplary non-viral mediated techniques include, but are not limited to, electroporation, calcium phosphate mediated transfection, nucleofection, sonoporation, heat shock, magnetic infection, liposome mediated transfection, microinjection, microprojectile mediated transfection (nanoparticles), cationic polymer mediated transfection (DEAE-dextran, polyethyleneimine, polyethylene glycol (PEG), etc.), or cell fusion. Other methods of transfection include proprietary transfection reagents such as LIPOFECTAMINE (Thermo Fisher Scientific, Inc., Waltham, MA), HILYMAX (Dojindo Molecular Technologies, Inc., Rockville, MD), FUGENE (Promega Corp., Maidion, WI), JETPEI (Polyplus Transfection, Illkirch, France), EFFECTENE (Qiagen, Hilden, Germany), and DreamFect (OZ Biosciences, Inc USA, San Diego, CA).

[0069] The nucleic acid constructs described herein can be introduced into modified host cells, thereby enabling intracellular expression of the protein encoded by the nucleic acid. A variety of host cells are known in the art and suitable for protein expression. Typical examples of cells used for transfection and protein expression include, but are not limited to, bacterial cells, eukaryotic cells, yeast cells, insect cells, or plant cells, such as E. coli, Bacillus, Streptomyces, Pichia pastoris, Salmonella typhimurium, Drosophila S2, Spodoptera SJ9, CHO, COS (e.g., COS-7), 3T3-F442A, HeLa, HUVEC, HUAEC, NIH 3T3, Jurkat, 293, 293H, or 293F.

[0070] In one or more embodiments, the antigenic ASC peptide can be chemically bound to an immunogenic carrier using techniques well known in the art. Conjugation can occur via single-point conjugation (e.g., at either the N- or C-terminus) to allow free movement of the peptide, or as a locked-down structure in which both ends of the peptide are conjugated to a scaffold structure such as an immunogenic carrier protein or VLP. Conjugation can occur via conjugation chemistries known to those skilled in the art, such as via cysteine ​​residues, lysine residues, or another carboxy moiety. Thus, for example, for direct covalent coupling, carbodiimide, glutaraldehyde, or N-[y-maleimidobutyloxy]succinimide ester can be used with common, commercially available heterobifunctional linkers such as 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) or 3-(2-pyridyldithio)succinimidyl propionate (SPDP).

[0071] Examples of conjugation of peptides, particularly cyclized peptides, to protein carriers via acylhydrazine peptide derivatives are described, for example, in International Patent Application No. PCT / EP2003 / 004551 (International Publication No. WO2003 / 092714 A1). After the coupling reaction, the immunogen can be easily isolated and purified using, for example, dialysis, high-performance liquid chromatography, gel filtration, fractionation, etc. Peptides terminating in a cysteine ​​residue (preferably with a linker outside the cyclization region) can be conveniently conjugated to carrier proteins via maleimide chemistry.

[0072] When the immunogenic carrier is a VLP, several antigenic peptides having either the same or different amino acid sequences can be bound to a single VLP particle, preferably resulting in a repetitive and ordered structure presenting several antigenic determinants in the orientation described in International Patent Applications PCT / IB1999 / 001925 (International Publication No. WO00 / 032227), PCT / IB2002 / 004132 (International Publication No. WO 2003 / 024481), PCT / IB2002 / 000166 (International Publication No. WO 02 / 056905), and PCT / EP2003 / 007572 (International Publication No. WO2004 / 007538). Thus, the antigenic peptide presented by one VLP subunit in a VLP can be the same as or different from the antigenic peptide presented by a second VLP subunit in the same VLP. In other embodiments, one or several antigen molecules can be bound to one VLP subunit. Thus, a specific feature of the VLPs of RNA phage coat proteins, especially Qβ coat protein VLPs, is the possibility of binding several antigens per subunit. This allows the generation of high-density antigen arrays.

[0073] Another feature of VLPs derived from RNA phages is their high expression yield in bacteria, which allows for the production of large amounts of material at reasonable cost. Furthermore, the use of VLPs as carriers allows for the formation of robust antigen arrays and conjugates with variable antigen densities. In particular, the use of VLPs from RNA phages, especially VLPs from RNA phage Qβ coat protein, allows for the achievement of very high antigen densities.

[0074] ASC-targeted VLPs can be used to treat subjects having or at risk of having an inflammatory condition associated, at least in part, with inflammasome assembly and / or inflammatory cytokines.

[0075] As used herein, "treat" or variations thereof refers to reducing, limiting the progression of, ameliorating, or resolving to any extent, the symptoms or signs associated with a condition. A "sign" or "clinical sign" refers to an objective physical finding associated with a particular condition that can be detected by someone other than the subject. A "symptom" refers to any objective evidence of a disease or condition in a subject.

[0076] "Treatment" can be therapeutic or prophylactic. "Therapeutic" and its variations refer to treatment that ameliorates one or more existing symptoms or clinical signs associated with a condition. "Prophylactic" and its variations refer to treatment that limits, to any extent, the onset and / or appearance of symptoms or clinical signs of a condition. Generally, "therapeutic" treatment is initiated after a condition is manifested in a subject, while "prophylactic" treatment is initiated before a condition is manifested in a subject.

[0077] Preventive treatment, for example, treatment that is initiated before the subject shows symptoms or clinical signs of a pathological condition, for example, while the inflammatory condition remains asymptomatic, is referred to herein as treatment of a subject "at risk" of having a pathological condition. As used herein, the term "at risk" refers to a subject who may or may not actually have the described risk. Thus, for example, a subject "at risk" of developing a condition is a subject who has one or more risk factors associated with the pathological condition, such as genetic predisposition, ancestry, age, sex, geographic location, lifestyle, or medical history. Thus, ASC-targeted VLPs can be administered prophylactically before the subject shows symptoms or clinical signs of an inflammatory condition.

[0078] Thus, the composition can be administered before, during, or after the subject first exhibits symptoms or clinical signs of an inflammatory condition. Treatment initiated before the subject first exhibits symptoms or clinical signs associated with an inflammatory condition can result in a reduced likelihood that the subject will experience clinical evidence of the inflammatory condition, a reduced severity of the symptoms and / or clinical signs of the condition, and / or a complete recovery of the inflammatory condition, compared to a subject not administered the composition. Treatment initiated after the subject first exhibits symptoms or clinical signs associated with an inflammatory condition can result in a reduced severity of the symptoms and / or clinical signs of the inflammatory condition, and / or a complete recovery of the inflammatory condition, compared to a subject not administered the composition.

[0079] Thus, the method includes administering an effective amount of the composition to a subject having or at risk of having an inflammatory condition involving inflammasome assembly and / or inflammatory cytokines. In this aspect, an "effective amount" is an amount effective to any extent to reduce, limit the progression of, ameliorate, or resolve symptoms or clinical signs associated with the inflammatory condition.

[0080] Thus, the ASC-targeted Qβ VLPs described herein can be formulated with a pharmaceutically acceptable carrier. As used herein, "carrier" includes any solvent, dispersion medium, vehicle, coating agent, diluent, antibacterial and / or antifungal agent, isotonicity agent, absorption delaying agent, buffer, carrier liquid, suspension, colloid, etc. The use of such media and / or agents for pharmaceutically active substances is known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the present pharmaceutical compositions is contemplated. Supplementary active ingredients can also be incorporated into the composition. As used herein, "pharmaceutically acceptable" refers to a material that is not biologically or otherwise undesirable, i.e., the material can be administered to an individual together with the ASC-targeted Qβ VLPs without causing any undesired biological effects or interacting adversely with any other components of the pharmaceutical composition in which it is included.

[0081] Therefore, ASC-targeted Qβ VLPs can be formulated into pharmaceutical compositions. Pharmaceutical compositions can be formulated in various forms compatible with the preferred route of administration. Thus, the compositions can be administered via known routes, including, for example, oral, parenteral (e.g., intradermal, transdermal, subcutaneous, intramuscular, intravenous, intraperitoneal, etc.), or topical (e.g., intranasal, intrapulmonary, intramammary, intravaginal, intrauterine, rectal, etc.). Pharmaceutical compositions can be administered to mucosal surfaces, for example, by administration to the nasal or respiratory mucosa (e.g., by spray or aerosol). The compositions can also be administered via sustained or delayed release.

[0082] Therefore, ASC-targeted Qβ VLPs can be provided in any suitable form, including, but not limited to, a solution, a suspension, an emulsion, a spray, an aerosol, or any mixture. The composition can be delivered in a formulation using any pharmaceutically acceptable excipient, carrier, or vehicle. For example, the formulation can be delivered in a conventional topical administration form, such as a cream, an ointment, an aerosol formulation, a non-aerosol spray, a gel, a lotion, or the like. The formulation can further include one or more additives, including, for example, an adjuvant (whether an ADVAX adjuvant or other adjuvant), a skin penetration enhancer, a colorant, a fragrance, a flavoring, a moisturizer, a thickener, etc.

[0083] The formulations may conveniently be presented in unit dosage form and may be prepared by any methods well known in the art of pharmacy. Methods of preparing compositions using pharmaceutically acceptable carriers include the step of bringing the ASC-targeted Qβ VLPs into association with the carrier, which constitutes one or more accessory ingredients. In general, the formulations may be prepared by uniformly and / or intimately bringing the active ingredient into association with liquid carriers or finely divided solid carriers, or both, and then, if necessary, shaping the product into the desired formulation.

[0084] The amount of ASC-targeted Qβ VLP administered may vary depending on various factors, including, but not limited to, the inflammatory condition being treated, the subject's weight, health condition, and / or age, and / or the route of administration. Thus, the absolute weight of ASC-targeted Qβ VLP contained in a given unit dosage form may vary widely and depend on factors such as the subject's species, age, weight, and health condition, and / or the method of administration. Therefore, it is not practical to generally state the amount that constitutes an effective amount of ASC-targeted Qβ VLP for all possible applications. However, those skilled in the art can easily determine the appropriate amount with due consideration of such factors.

[0085] In one or more embodiments, the method may comprise administering to the subject sufficient ASC-targeted Qβ VLP to provide a dose of, for example, about 50 ng / kg to about 1 mg / kg, although in one or more embodiments the method may be carried out by administering ASC-targeted Qβ VLP at a dose outside this range.

[0086] dosage In one or more embodiments, the method comprises administering sufficient ASC-targeted Qβ VLPs to provide a minimum dose of at least 50 ng / kg, e.g., at least 100 ng / kg, at least 200 ng / kg, at least 300 ng / kg, at least 400 ng / kg, at least 500 ng / kg, at least 600 ng / kg, at least 700 ng / kg, at least 800 ng / kg, at least 900 ng / kg, at least 1 μg / kg, at least 2 μg / kg, at least 5 μg / kg, at least 10 μg / kg, at least 20 μg / kg, at least 50 μg / kg, at least 100 μg / kg, at least 200 μg / kg, or at least 500 μg / kg.

[0087] In one or more embodiments, the methods comprise administering sufficient ASC-targeted Qβ VLP to provide a maximum dose of 1 mg / kg or less, 500 μg / kg or less, 250 μg / kg or less, 200 μg / kg or less, 150 μg / kg or less, 100 μg / kg or less, 50 μg / kg or less, 25 μg / kg or less, 10 μg / kg or less, 5 μg / kg or less, 2 μg / kg or less, 1 μg / kg or less, 800 ng / kg or less, 600 ng / kg or less, 500 ng / kg or less, 400 ng / kg or less, 300 ng / kg or less, 250 ng / kg or less, 150 ng / kg or less, 100 ng / kg or less, 50 ng / kg or less, or 25 ng / kg or less.

[0088] In one or more embodiments, the method comprises administering sufficient ASC-targeted Qβ VLP to provide a dose that falls within a range having as endpoints any minimum dose recited above and any maximum dose greater than the minimum dose recited above. For example, in one or more embodiments, the method may comprise administering to the subject sufficient ASC-targeted Qβ VLP to provide a dose of 200 ng / kg to about 10 μg / kg, for example, about 700 ng / kg to about 5 μg / kg.

[0089] In one or more embodiments, ASC-targeted Qβ VLPs can be administered in multiple doses, for example, once a week, although in one or more embodiments, the method can be performed by administering ASC-targeted Qβ VLPs at frequencies outside this range. When multiple doses are used within a certain period of time, the amount of each dose can be the same or different. For example, a 1 mg dose per day can be administered as a single 1 mg dose, two 0.5 mg doses, or a first 0.75 mg dose followed by a second 0.25 mg dose. Also, when multiple doses are used within a certain period of time, the intervals between doses can be the same or different.

[0090] Dosage frequency In certain embodiments, ASC-targeted Qβ VLPs may be administered at a minimum frequency of at least once a year, such as, for example, at least once every 6 months, at least once every 4 months, at least once every 3 months, at least once every 2 months, at least once a month, or at least once every 2 weeks.

[0091] In certain embodiments, ASC-targeted Qβ VLPs may be administered at a maximum frequency of not more than once per week, such as, for example, not more than once every two weeks, not more than once per month, not more than once every two months, not more than once every three months, not more than once every six months, or once per year.

[0092] In one or more embodiments, ASC-targeted Qβ VLPs may be administered at a frequency defined by a range having as its endpoints any minimum frequency described above and any maximum frequency described above that is higher than the minimum frequency.

[0093] Treatment duration The duration of administration of the antigenic ASC peptides described herein, e.g., the period over which the antigenic ASC peptide is administered, can vary depending on any of a variety of factors, such as patient response. For example, the antigenic ASC peptide can be administered for a period ranging from about 1 day to about 1 week, about 2 weeks to about 4 weeks, about 1 month to about 2 months, about 2 months to about 4 months, about 4 months to about 6 months, about 6 months to about 8 months, about 8 months to about 1 year, about 1 year to about 2 years, or about 2 years to about 4 years, or longer. In one or more embodiments, the ASC-targeted Qβ VLPs can be administered as a one-time treatment. In other embodiments, the ASC-targeted Qβ VLPs can be administered for the lifetime of the subject. In certain embodiments, the ASC-targeted Qβ VLPs can be administered monthly (e.g., every 4 weeks) until efficacy is demonstrated.

[0094] In some cases, ASC-targeted Qβ VLPs may be administered at an initial frequency for an initial period, and then at a lower frequency thereafter. For example, a dosing regimen may include administering ASC-targeted Qβ VLPs three times monthly (i.e., a first dose followed by a second dose one month after the first dose), followed by an additional dose six months after the first dose.

[0095] When the ASC-targeted Qβ VLP composition is used for prophylactic treatment, it can generally be administered in a priming dose and / or a boosting dose. If administered, boosting doses are administered at appropriate intervals (e.g., annually) to boost circulating antibody levels that have fallen below the desired level. The boosting dose can contain the ASC-targeting peptide with or without the original immunogenic carrier. The booster composition can contain an alternative immunogenic carrier or can contain no carrier. Furthermore, the booster composition can be formulated with or without an adjuvant.

[0096] In one exemplary embodiment, the ASC-VLP vaccine may be administered in a three-dose regimen, with the three doses administered at one-month and six-month intervals, or in a range of one to twelve months. If necessary, additional boosters may be administered at an appropriate frequency (e.g., every 12 months). In one exemplary embodiment, the treatment regimen may include a first dose of 100 μg of VLPs or in the range of 50 μg to 300 μg of VLPs, and repeated booster doses of 50 μg to 300 μg of VLPs (e.g., 100 μg of VLPs).

[0097] In one or more embodiments, the ASC-VLP vaccine can be combined with one or more additional therapeutic agents. The one or more additional therapeutic agents may be administered before, after, and / or simultaneously with the administration of the ASC-VLP vaccine. The ASC-VLP vaccine and the additional therapeutic agent may be co-administered. As used herein, "co-administered" refers to the administration of two or more components of a combination such that the therapeutic or prophylactic effect of the combination may be greater than the therapeutic or prophylactic effect of either component administered alone. The two components may be co-administered simultaneously or sequentially. Components co-administered simultaneously may be provided in one or more pharmaceutical compositions. Sequential co-administration of two or more components includes when the components are administered such that each component is present at the treatment site at the same time. Alternatively, sequential co-administration of two components may include when at least one component is excluded from the treatment site, but at least one cellular effect resulting from the administration of the component (e.g., inactivation of inflammasomes, inhibition of one or more inflammatory cytokines, etc.) persists at the treatment site until one or more additional components are administered to the treatment site. Thus, co-administered combinations may, in certain circumstances, include components that are not present in chemical mixtures with each other. In one or more alternative embodiments, the ASC-VLP vaccine and additional therapeutic agent(s) may be administered as part of a mixture or cocktail. In some aspects, administration of an ASC-VLP vaccine may allow for the effectiveness of lower dosages of other therapeutic modalities compared to administration of the other therapeutic agent(s) alone, thereby reducing the likelihood, severity, and / or extent of toxicity observed when higher doses of the other therapeutic agent(s) are administered.

[0098] Exemplary additional therapeutic agents include, but are not limited to, anti-ASC antibody preparations.Exemplary anti-ASC antibody preparations include, but are not limited to, antibodies against full-length ASC (e.g., mAb, 04-147, clone 2EI-7, 1:1,000 dilution, MilliporeSigma, Burlington, MA), antibodies against CARD region (e.g., mAb, 653902, clone TMS-1, 1:500 dilution, BioLegend, San Diego, CA, pAb, sc-22514-R, clone N-15R, 1:200 dilution, Santa Cruz Biotechnology, Inc., Dallas, TX).

[0099] In the foregoing description and in the claims that follow, the term "and / or" means one or all of the listed elements or a combination of any two or more of the listed elements, and the terms "comprises," "comprising," and variations thereof, are to be construed as open-ended, i.e., additional elements or steps are optional and may or may not be present. Unless otherwise specified, "a," "an," "the," and "at least one" are used interchangeably and mean one or more, and the recitation of numerical ranges by endpoints includes all numbers subsumed within that range (e.g., 1 to 5 includes 1, 1.5, 2, 2.75, 3, 3.80, 4, 5, etc.).

[0100] In the above description, certain embodiments may be described in isolation for clarity. Throughout this specification, references to, for example, “one embodiment,” “an embodiment,” “certain embodiments,” “some embodiments,” or “one or more embodiments” mean that a particular feature, structure, composition, or characteristic described in connection with an embodiment is included in at least one embodiment of the present disclosure. Thus, the appearance of such phrases in various places throughout this specification does not necessarily refer to the same embodiment of the present disclosure. Furthermore, particular features, structures, compositions, or characteristics may be combined in any suitable manner in one or more embodiments. Furthermore, particular features, structures, compositions, or characteristics may be combined in any suitable manner in one or more embodiments. Thus, features described in the context of one embodiment may be combined with features described in the context of a different embodiment, unless the features are necessarily mutually exclusive.

[0101] For any method disclosed herein that includes separate steps, the steps can be performed in any feasible order, and, where appropriate, any combination of two or more steps can be conducted simultaneously.

[0102] As used herein, the terms "preferred" and "preferably" refer to embodiments of the invention that may offer certain advantages, under particular circumstances. However, other embodiments may also be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, and is not intended to exclude other embodiments from the scope of the invention.

[0103] The present invention is illustrated in the following examples, it being understood that the particular examples, materials, amounts, and procedures are to be interpreted broadly in accordance with the scope and spirit of the invention set forth herein. [Example]

[0104] Qβ-VLPs were produced in Escherichia coli (E. coli) using a previously described method (Crossey, E. et al. Vaccine. 2015). The ASC peptide was synthesized (GenScript Biotech Corp., Piscataway, NJ) and stored as a lyophilized powder at -20°C. The ASC peptide was conjugated to surface-exposed lysines of assembled VLPs using the bifunctional crosslinker succinimidyl 6-[(beta-maleimidopropionamido)hexanoate] (SMPH, Thermo Fisher Scientific, Inc., Waltham, MA) (Figure 3). The efficiency of conjugation was confirmed by mobility-shift gel electrophoresis on a 10% SDS-denaturing polyacrylamide gel (Figure 4).

[0105] C57Bl / 6J mice were obtained from The Jackson Laboratory (Bar Harbor, ME) and aged to 2 months of age. Mice were randomized according to sex and assigned to either Qβ control sham treatment, Qβ ASC-linker, Qβ ASC-helix 4, or Qβ ASC-C-terminus vaccine treatment (n = 5 per group). Vaccines were administered at 2 months of age with a dose of 5 μg of VLPs (100–200 ng / μL concentration) suspended in 50 μL of sterile water (10 μg for ASC-C-terminus due to poor conjugation efficiency), followed by a booster vaccine 3 weeks later via intramuscular injection into the hind leg (Figure 5).

[0106] Three weeks after the second injection, plasma samples were obtained by retro-orbital capillary collection and antibody titers were assessed using an indirect enzyme-linked immunosorbent assay (ELISA) against the target peptide used to synthesize the vaccine. Immune serum from vaccinated mice was used as the primary antibody in multiple dilutions, and horseradish peroxidase-conjugated goat anti-mouse antibody was used with 3,3',5,5'-tetramethylbenzidine (TMB) substrate, detected by measuring absorbance at 450 nm (Figures 6-8).

[0107] Vaccinated animals were observed weekly for any signs of declining health, including poor grooming behavior, lethargy, dermatitis, sores, open wounds, or other evidence of infection. Animal weights were collected at four time points, including 2, 3, 5, and 6 months of age (Figure 9). At 6 months of age, blood samples were collected by retro-orbital capillary collection into ethylenediaminetetraacetic acid (EDTA) and heparin-coated tubes and then evaluated using a complete blood count on an Abaxis Vetscan HM5 (Figure 10) and a mouse blood chemistry panel on an Abaxis Vetscan VS2 (Figure 10).

[0108] Vaccinated animals were then injected intraperitoneally with 5 mg / kg lipopolysaccharide (LPS, derived from E. coli 055:B5, MilliporeSigma, Burlington, MA) and sacrificed 9 hours post-injection. Terminal blood collection was performed by cardiac puncture. Animals were then transcardially perfused with ice-cold 0.125 M phosphate buffer, and brains were microdissected and frozen on dry ice. Blood samples were allowed to clot on ice for 30 minutes, then centrifuged twice at 5000 RPM for 5 minutes to isolate immune serum and store at -80°C. Serum interleukin-1β levels were assessed using a mouse IL-1β DuoSet ELISA kit (R&D systems, catalog no. DY401) by diluting serum 50% with reagent diluent (R&D Systems, Inc., Minneapolis, MN) according to the manufacturer's instructions (Figure 14).

[0109] Hippocampal brain tissue samples were homogenized in 10% w / v tissue protein extraction reagent (T-PER, Thermo Fisher Scientific, Inc., Waltham, MA) containing 1% protease and phosphatase inhibitors (Thermo Fisher Scientific, Inc., Waltham, MA). Cells were lysed in 1x LDS / RA buffer (Thermo Fisher Scientific), sonicated for 30 seconds, and boiled at 95°C for 15 minutes. Lysates were separated via SDS-PAGE on a 4-12% Bis-Tris gradient gel and immunoblotted using caspase-1 p20 (AdipoGen Life Sciences, Inc., San Diego, CA) and GAPDH (MilliporeSigma, Burlington, MA) (Figure 15).

[0110] Formalin-fixed, paraffin-embedded human Alzheimer's disease hippocampal brain sections (n ​​= 1) were used for immunohistochemical analysis of the immunoreactivity of ASC-VLP immune serum. The sections were first sequentially hydrated with xylene, ethanol, and PBS containing 0.1% Tween® (PBST), then incubated in 10 mM sodium citrate buffer (pH 6.0) at 95°C for 30 minutes to retrieve antigens. They were then washed with PBS containing 0.1% Tween® (PBST) and quenched with 0.3% H2O2 in PBST for 20 minutes. The sections were blocked with 5% normal goat serum for 1 hour at room temperature. The sections were then incubated with rabbit polyclonal anti-ASC antibody (1:500, AdipoGen Life Sciences, Inc., San Diego, CA) or immune serum from Qβ control or Qβ-ASC VLP-vaccinated animals (1:500). After washing in PBST, sections were incubated with biotinylated secondary antibody (1:250, Jackson ImmunoResearch Laboratories Inc., West Grove, PA). Sections were then incubated with ABC (Vector Laboratories, Inc., Newark, CA) reagent for 30 minutes at room temperature. Immunoreactive signals were revealed by developing sections with SIGMAFAST 3,3'-diaminobenzidine (DAB) tablets (MilliporeSigma, Burlington, MA). Slides were dehydrated in ethanol and xylene, then coverslipped and imaged using a bright-field microscope (Figure 11).

[0111] Non-vaccinated C57Bl6 / J mice (n=4) injected with 5 mg / kg LPS (as above) and sacrificed 9 hours later, ASC - / -Hippocampal brain lysates from a transgenic mouse (n = 1) and a human Alzheimer's disease patient (n = 4) were used to assess the cross-reactivity of immune serum antibodies between human and mouse ASC. Hippocampal brain lysates were separated by SDS-PAGE as described above and immunoblotted with rabbit polyclonal anti-ASC antibody (1:2000, AdipoGen Life Sciences, Inc., San Diego, CA) or immune serum from Qβ control or Qβ-ASC VLP immune serum (1:1000) (Figure 12). Indirect sandwich ELISA was performed using rabbit polyclonal anti-ASC antibody (1:200, AdipoGen Life Sciences, Inc., San Diego, CA) as the capture antibody, incubating with hippocampal brain lysates from mice and humans at room temperature for 1 hour, and using Qβ control or Qβ-ASC VLP immune serum as the detection antibody, followed by detection of bound anti-ASC antibody using HRP-conjugated secondary antibody (1:10,000, Jackson ImmunoResearch Laboratories Inc., West Grove, PA) with TMB substrate absorbance at 450 nm (Figure 13).

[0112] The complete disclosures of all patents, patent applications, and publications cited herein, as well as electronically available materials (including, by way of example, nucleotide sequence submissions in, e.g., GenBank and RefSeq, and amino acid sequence submissions in, e.g., SwissProt, PIR, PRF, PDB, and translations from the annotated coding regions of GenBank and RefSeq), are incorporated by reference in their entirety. In the event of a discrepancy between the disclosure of this application and the disclosure(s) of any document incorporated herein by reference, the disclosure of this application shall control. The foregoing detailed description and examples have been given for clarity of understanding only. No unnecessary limitations should be understood. The invention is not limited to the exact details shown and described, and variations obvious to those skilled in the art will be within the scope of the invention as defined by the claims.

[0113] Unless otherwise noted, all numbers expressing amounts of ingredients, molecular weights, and the like used in the specification and claims should be understood to be modified in all instances by the term "about." Accordingly, unless otherwise indicated, the numerical parameters set forth in the specification and claims are approximations and may vary depending upon the desired properties sought to be obtained by the present invention. At the very least, and not as an attempt to limit the application of the doctrine of equivalents to the scope of the claims, each numerical parameter should at least be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.

[0114] Notwithstanding that the numerical ranges and parameters setting forth the broad scope of the invention are approximations, the numerical values ​​set forth in the specific examples are reported as precisely as possible. Any numerical value, however, inherently contains some range necessarily resulting from the standard deviation found in their respective testing measurements.

[0115] All headings are for the convenience of the reader and should not be used to limit the meaning of the text that follows the heading, unless specifically stated.

[0116] Sequence Listing Free Text SEQ ID NO: 1 - human ASC sequence [ka] Pyrin domain: 1-91 CARD domain: 107-195

[0117] SEQ ID NO:2 - Mouse ASC sequence [ka] Pyrin domain: 1-91 CARD domain: 105~193

[0118] SEQ ID NO:3 - ASC linker peptide GSGAAPAGIQ APPQS

[0119] SEQ ID NO: 4 - ASC helix 4 peptide NPSKMRKLFS FTPAWNWTC

[0120] SEQ ID NO:5 CKDLLLQALR ESQSYLVEDL ERS

[0121] SEQ ID NO: 6 - human ACS peptide sequence MGRARDAIL DALENL

[0122] SEQ ID NO:7 - Human ACS peptide sequence ELKKFKLKLL SV

[0123] SEQ ID NO:8 - Human ACS peptide sequence REGYGRIPRG ALL

[0124] SEQ ID NO: 9 - human ACS peptide sequence DALDLTDKLV SFY

[0125] SEQ ID NO: 10 - human ACS peptide sequence DQHRAALIAR

[0126] SEQ ID NO: 11 - human ACS peptide sequence TNVEWLLDAL Y

[0127] SEQ ID NO: 12 - human ACS peptide sequence DEQYQAVRAE

[0128] SEQ ID NO: 13 - Mouse ACS peptide sequence ELKKFKMKLL TV

[0129] SEQ ID NO: 14 - Mouse ACS peptide sequence QLREGYGRIP RGALL

[0130] SEQ ID NO: 15 - Mouse ACS peptide sequence DAIDLTDKLV SYY

[0131] SEQ ID NO: 16 - Mouse ACS peptide sequence DQHRQALIAR

[0132] SEQ ID NO: 17 - Mouse ACS peptide sequence TEVDGVLDAL

[0133] SEQ ID NO: 18 - Mouse ACS peptide sequence EGQYQAVRAE

[0134] SEQ ID NO: 19 - Mouse ACS peptide sequence DKMRKLFSFV PSWN

[0135] SEQ ID NO: 20 - Mouse ACS peptide sequence KEIHPYLVMD LEQS

[0136] SEQ ID NO: 21 - Mouse ACS peptide sequence AAAASVPAQS TAR

[0137] SEQ ID NO: 22 - linker GGGC

[0138] SEQ ID NO: 23 - linker CGGG

Claims

1. It is an immunogen, An immunogenic carrier containing Qβ bacteriophage virus-like particles (VLPs), and Antigenic apoptosis-related speck-like protein containing caspase activation and recruitment domain (ASC) peptides linked to the immunogenic carrier. The immunogen, including the immunogen.

2. The immunogen according to claim 1, wherein the ASC peptide comprises the amino acid sequence of SEQ ID NO: 1 or an antigenic fragment thereof.

3. The immunogen according to claim 2, wherein the fragment of SEQ ID NO: 1 comprises SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, or SEQ ID NO:

12.

4. The immunogenic carrier is linked to the ASC peptide via a succinimidyl-6-[β-maleimidopropionamide]hexanoate (SMPH) crosslinking agent molecule, according to any one of claims 1 to 3.

5. The immunogen according to any one of claims 1 to 3, further comprising a second antigenic ASC peptide.

6. The immunogen according to claim 5, wherein both ASC peptides are presented on a single VLP.

7. A composition comprising the immunogen according to any one of claims 1 to 3.

8. A composition comprising the immunogen described in Claim 5.

9. The composition according to claim 7, The first population of VLPs presenting the first antigenic ASC peptide, and The second population of VLPs presenting the second antigenic ASC peptide The composition comprising the above.

10. The composition according to claim 7, further comprising an adjuvant.

11. The composition according to claim 8, further comprising an adjuvant.

12. A pharmaceutical composition for treating an inflammatory condition in a subject, comprising an immunogen, wherein the immunogen is An immunogenic carrier containing Qβ bacteriophage virus-like particles (VLPs), and Antigenic apoptosis-related speck-like protein containing caspase activation and recruitment domain (ASC) peptides linked to the immunogenic carrier. A pharmaceutical composition containing the following:

13. The pharmaceutical composition according to claim 12, for use in combination with at least one additional therapeutic agent for treating an inflammatory condition.

14. The pharmaceutical composition according to claim 12 or claim 13, wherein the immunogen further comprises a second antigenic ASC peptide.

15. The pharmaceutical composition according to claim 14, wherein both antigenic ASC peptides are linked to a single carrier.

16. A pharmaceutical composition according to claim 14, wherein the pharmaceutical composition is The first group of immunogens, The first group of immunogenic carriers, and The first group of immunogens includes a first antigenic ASC peptide linked to the first group of immunogenic carriers, and The second group of immunogens, The second group of immunogenic carriers, and The second group of immunogens comprises a second ASC peptide linked to the second group of immunogenic carriers. The pharmaceutical composition comprising the above.

17. The pharmaceutical composition according to claim 12 or 13, wherein at least one antigenic ASC peptide comprises the amino acid sequence of SEQ ID NO: 1 or an antigenic fragment thereof.

18. The pharmaceutical composition according to claim 17, wherein the antigenic fragment of SEQ ID NO: 1 comprises SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, or SEQ ID NO:

12.

19. The pharmaceutical composition according to claim 12 or 13, for administration to the subject before the subject shows symptoms or clinical signs of an inflammatory state.

20. A nucleic acid encoding an immunogen as described in claim 1.

21. An expression vector comprising the nucleic acid described in claim 20.

22. A host cell comprising the expression vector according to claim 21.

23. A vaccine comprising the immunogen described in any one of claims 1 to 3.

24. A vaccine comprising the composition described in Claim 7.

25. A vaccine comprising the composition according to claim 8.

26. A pharmaceutical composition for treating an inflammatory condition in a subject, comprising a therapeutically effective amount of the vaccine described in claim 23.

27. ​​The pharmaceutical composition according to claim 26, for use in combination with at least one additional therapeutic agent for treating the inflammatory condition.

28. The pharmaceutical composition according to claim 26 or claim 27, which is administered to the subject before the subject shows symptoms or clinical signs of inflammation.

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