Method for collecting skin surface material, method for examining skin surface material, and sediment

Abstract

To provide a method for efficiently collecting skin surface substances, which are endogenous substances present on the stratum corneum of the skin, using a method that allows for long-term application. [Solution] An adhesion step is performed in which a deposit containing fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less is applied to the skin and held for 0.5 hours or more to cause skin surface substances, which are internally derived substances present on the stratum corneum of the skin, to adhere to the deposit. A method for collecting surface skin matter, comprising a removal step of removing the deposit that has undergone an adhesion step from the skin, and recovering the deposit after the removal step to which surface skin matter is attached.

Description

[Technical Field]

[0001] This invention relates to a method for collecting surface skin material, a method for examining surface skin material, and a deposit. [Background technology]

[0002] In recent years, attempts have been made to collect and analyze biomarkers and immune response proteins from skin tissue using the skin surface. For example, Non-Patent Document 1 proposes a method called skin blotting. This document states that "within the skin, there are markers (proteins, etc.) that reflect the overall health status and markers that represent the local condition of the skin in the interstitial fluid (body fluid) that seeps out from the blood vessels (Figure 1). Skin blotting is a technique that extracts these markers simply by moistening a 1 cm square skin patch (a tape that is electrostatically charged and attracts markers) and applying it to the skin for 10 minutes (Figure 2)." According to Non-Patent Document 1, this method makes it possible to monitor the health status inside the body without performing blood sampling. Patent Document 1 describes how, using a skin blotting method to detect pressure ulcer prediction markers, it was shown that there are differences in the expression of immune response proteins between the skin of mice to which voltage has been applied for a long period of time and the skin of control mice.

[0003] Patent Document 2 describes a cosmetic method for protecting the skin, which includes a step (adhesion step) of applying a coating made of a deposit containing fibers with an average fiber diameter of 0.01 to 7 μm to the skin and holding it for 1 to 12 hours. The same document describes an analysis of sebum attached to cigarette paper after wiping the skin with cigarette paper before and after the formation of the coating (paragraphs

[0094] and

[0096] ). [Prior art documents] [Patent Documents]

[0004] [Patent Document 1] International Publication No. 2017 / 122780 [Patent Document 2] Japanese Patent Publication No. 2022-074055 [Non-patent literature]

[0005] [Non-Patent Document 1] https: / / www.ishikawa-nu.ac.jp / lab / bioengnurs / introduction / (Accessed May 20, 2024) [Overview of the project] [Problems that the invention aims to solve]

[0006] The skin blotting method described in Non-Patent Document 1 involves application using an adhesive sheet, which can easily cause stress to the skin during application and removal. The skin blotting method described in Patent Document 1 shows the application condition under conditions where fluid easily seeps from within the skin, such as in pressure ulcers. Neither method can be said to demonstrate the effectiveness of routinely collecting internally derived components while reducing stress on the skin. Patent Document 2 does not contain any mention of the problem of removing deposits of fibers of a specific fiber diameter from the skin and then collecting them as a source of internally derived components.

[0007] The present invention aims to provide a method for efficiently collecting skin surface substances, which are endogenous substances present on the stratum corneum of the skin, using a method that allows for long-term application. [Means for solving the problem]

[0008] This invention provides a method for collecting skin surface material. In one embodiment, a deposit containing fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less is applied to the skin. In one embodiment, the deposit is kept on the skin for 0.5 hours or more, causing skin surface substances, which are internally derived substances present on the stratum corneum of the skin, to adhere to the deposit. In one embodiment, a deposit with surface material attached is removed from the skin. In one embodiment, deposits with skin surface substances attached thereto, which are removed from the skin, are collected.

Effects of the Invention

[0009] The present invention can provide a method for collecting skin surface substances that can efficiently collect skin surface substances, which are substances derived from the body and present on the stratum corneum of the skin, by a method that can be attached for a long time.

Brief Description of the Drawings

[0010] [Figure 1] FIG. 1 is a schematic diagram showing the configuration of an electrostatic spray device used in the present invention. [Figure 2] FIG. 2 is a schematic diagram showing a state of performing an electrostatic spraying method using an electrostatic spray device. [Figure 3] FIG. 3 is a graph showing the peak area of albumin in the mass spectrometry of skin surface substances obtained by the collection methods of Examples and Comparative Examples. [Figure 4] FIG. 4 shows the results of the peak area ratio (ALB / KR10) of albumin and keratin-10 in the mass spectrometry of skin surface substances obtained by the collection methods of Examples and Comparative Examples. [Figure 5] FIG. 5 shows the results of the protein identification number (FDR5%) in the mass spectrometry of skin surface substances obtained by the collection methods of Examples and Comparative Examples.

Modes for Carrying Out the Invention

[0011] Hereinafter, preferred embodiments of the present invention will be described. In the present invention, the skin surface substances refer to substances derived from the body that are present on the stratum corneum of the skin. The stratum corneum constitutes the outermost surface of the skin. Further, the components derived from the body refer to components derived from the body of the owner of the skin. The "components derived from the body" as referred to in this specification is intended to exclude externally attached components. Examples of skin surface materials that can be collected by the present invention include proteins, nucleic acids, lipids, sugars, and amino acids. Furthermore, in the collection method of the present invention, it is preferable to collect one or more selected from proteins, nucleic acids, and lipids. The proteins referred to here preferably include one or more selected from immune response proteins, natural moisturizing factor-producing enzymes, ceramide metabolic enzymes, stratum corneum exfoliating proteases, oxidoreductases, desmosome constituent proteins, and plasma-derived proteins. Examples of immune response proteins include antimicrobial peptides, cytokines, chemokines, immunoglobulins, complement system proteins, and fibrinogen. Examples of enzymes that produce natural moisturizing factors include filaggrin-degrading enzymes, aminopeptidases, and Peptidyl Arginine Deiminase 1. Examples of ceramide metabolic enzymes include β-glucocerebrosidase, sphingomyelinase, ceramidase, glucosylceramide synthase, ceramide synthase, and serine palmitoyltransferase. Examples of oxidoreductases include SOD, peroxidase, catalase, glutathione reductase, thioredoxin reductase, NOX, LOX, COX, aldose reductase, and tyrosinase. Desmosome constituent proteins include desmoglein, desmocolin, placoglobin, placofilin, and desmoplakin. Examples of plasma-derived proteins include albumin and transferrin. Nucleic acids include DNA and RNA. Lipids include sebum. Sugars and amino acids may be present in the skin surface as metabolites. Metabolites include those of bacteria and other organisms on the skin surface. The proteins that can be collected as skin surface material by this invention are thought to have seeped onto the surface of the stratum corneum from interstitial fluid, sweat glands, hair follicles, sebaceous glands, etc. Their origin is thought to be from these organs, as well as from blood and internal skin tissue. Similarly, nucleic acids are thought to have seeped onto the surface of the stratum corneum from interstitial fluid, sweat glands, hair follicles, sebaceous glands, etc. In addition, sebum is secreted onto the surface of the stratum corneum by the sebaceous glands. Interstitial fluid is normally released from the human body in small amounts over time. However, there has been no conventional method to capture and store this small amount of released interstitial fluid as it is released. By attaching fibers with a specific fiber diameter as described herein to the skin for an extended period, it becomes possible to retain the small amount of released interstitial fluid between the fibers over a long period. The small amount of released interstitial fluid accumulates between the fibers over a long period, eventually reaching the amount necessary for analysis. As a result, the deposit becomes a sample containing interstitial fluid. This accumulated interstitial fluid is composed of internally derived components and is distinct from stratum corneum-derived components immediately wiped away with oil-blotting paper, etc. If these internally derived components are indeed interstitial fluid, then its application as a non-invasive method for collecting interstitial fluid is expected. Furthermore, internally derived components obtained from interstitial fluid can be found to have components equivalent to those found in blood, so applications such as testing interstitial fluid instead of blood tests are expected. The advantage of this alternative test is that, unlike blood tests which use invasive collection methods, this alternative test can be performed using a non-invasive method such as attaching a sample to the skin. In this specification, "non-invasive sampling method" means collecting skin tissue without performing procedures that involve incision, puncture, or other tissue damage to the subject's skin. For example, a method of capturing skin tissue by applying a fibrous deposit to the skin surface is included in non-invasive sampling methods.

[0012] The inventors believe that the proteins collected as skin surface material in this invention may have existed on the surface of the stratum corneum all along. However, they believe that their existence was not widely known until now because a suitable collection method had not been developed. The inventors believe that one reason why skin tissue can be efficiently collected using this invention is the effect of capillary action due to the use of fibers of a specific fiber diameter. Because this invention allows for the collection of a certain amount or more of skin tissue, the analytical device for the collected skin tissue is not limited to a high-precision measuring device, but can also be a simple measuring device. Fibers of a specific diameter can adhere to the skin for extended periods. This is because the fine fibers conform to the contours of the skin. In addition, the large specific surface area of ​​the fibers increases adhesion to the skin, which also contributes to their long-lasting adhesion.

[0013] The present invention's method for collecting skin surface material preferably includes an adhesion step. In the adhesion step, it is preferable to apply a deposit containing fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less to the skin. In the adhesion step, after applying the deposit to the skin, it is preferable to maintain the state in which the deposit is in contact with the skin for 0.5 hours or more to allow the skin surface material to adhere to the deposit. In this specification, the adhesion step includes not only applying the deposit to the skin but also subsequent maintenance. In the following, "deposits containing fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less" may also be referred to as "fiber deposits" or simply "deposits." The deposits preferably contain 60% by mass or more of fibers with a fiber diameter of 0.01 μm or more and 7 μm or less, more preferably 70% by mass or more, even more preferably 80% by mass or more, and even more preferably 90% by mass or more.

[0014] The skin to which this invention can be applied includes the face, neck, hands, feet, back, shoulders, abdomen, chest, legs, and arms. Among these, the face, hands, and feet are preferred because they are less likely to be covered by clothing during the day, making them convenient for applying the deposit. The face and hands are particularly preferred because they allow for the collection of surface skin materials, as well as the cosmetic effects that have been known to be present in conventional fiber deposits. The face is particularly preferred because it is easy to collect many types of proteins as surface skin materials. The cheeks and / or forehead are especially preferred. Oil-blotting films and bandages are noticeable when applied to the face in daily life. On the other hand, the deposits of very fine fibers in this invention are less noticeable, and skin surface material can be collected in that state.

[0015] By applying a deposit containing fibers with an average fiber diameter of 0.01 μm to 7 μm to the skin, a film made of the deposit is formed on the skin. From the viewpoint of efficiently adhering skin surface materials to the sediment, the average fiber diameter of the fibers contained in the sediment is preferably 0.05 μm or more, and more preferably 0.1 μm or more. Furthermore, the average fiber diameter is preferably 5 μm or less, and more preferably 3 μm or less, from the viewpoint of maintaining the adhesion of the deposits to the skin. For example, natural cellulose fibers commonly used in oil-blotting papers are typically several tens of micrometers (10 μm or more) thick, and the same is true for the oil-blotting papers used in the comparative example described later.

[0016] The average fiber diameter is the average thickness of the fiber and is equivalent to the diameter of a circle. This fiber thickness can be measured by, for example, observing the fiber at a magnification of 10,000 times using a scanning electron microscope. Defects (fiber clumps, fiber intersections, droplets) are removed from the two-dimensional image, and 10 fibers are arbitrarily selected. A line perpendicular to the longitudinal direction of the fiber is drawn, and the fiber diameter can be measured by directly reading it. The average value of the fiber diameters of the 10 fibers is defined as the "average fiber diameter."

[0017] The length of the fibers is not particularly limited, but it is preferably 10 times or more the average fiber diameter, more preferably 20 times or more, and even more preferably 50 times or more. There is no particular upper limit to the length of the fibers, but if it is 100 times or more, it is defined as a continuous fiber, and any continuous fiber is acceptable.

[0018] The basis weight of the fiber deposit is 0.1 g / m². 2 Preferably, it is 0.4 g / m 2 It is more preferable that the amount be greater than or equal to 0.5 g / m 2 It is even more preferable that the value be greater than or equal to 0.8 g / m 2 It is even more preferable that the amount be greater than or equal to 1 g / m 2 It is particularly preferable that the amount be greater than or equal to the lower limit. Having a basis weight greater than or equal to the lower limit makes it easier to peel the deposits off the skin without damaging them. Furthermore, the basis weight of the fiber deposit is 50 g / m². 2 Preferably, it is 40 g / m 2It is more preferable that it is as follows, 30 g / m 2 It is even more preferable that it is as follows, 10 g / m 2 It is even more preferable that it is as follows. By setting the basis weight of the fiber deposit to be below the above upper limit, it becomes possible to apply the deposit to the skin for a long time.

[0019] The above deposit is typically in the form of a film. From the viewpoint of being easily peeled off from the skin without breaking the deposit, the thickness of the deposit is preferably 1 μm or more, more preferably 3 μm or more. Also, from the viewpoint of being able to apply the deposit to the skin for a long time, the thickness of the above deposit is preferably 300 μm or less, more preferably 100 μm or less, and even more preferably 20 μm or less. The thickness can be measured by a contact-type film thickness gauge, Mitutoyo Corporation's LiteMatic VL-50, with a measuring head with a super hard ball for general dial gauges (7.3 mm). The measuring force applied to the measurement object at regular intervals is set to 0.01 N. The deposits of each of the following examples are within the range of 1 μm or more and 100 μm or less, and in particular, the deposits of Examples 1 and 2 were within the range of 1 μm or more and 20 μm or less in thickness.

[0020] It is preferable that the fiber contains a thermoplastic resin from the viewpoint of easily manufacturing a deposit with a desired fiber diameter. Typically, the thermoplastic resin is a synthetic resin, and the fiber contains a synthetic resin as the main component. The main component means occupying 60% by mass or more and 100% by mass or less, preferably occupying 70% by mass or more, more preferably occupying 80% by mass or more, and even more preferably occupying 90% by mass or more. Thermoplastic resins are broadly classified into water-soluble polymers and water-insoluble polymers. In this specification, "water-soluble polymer" refers to a polymer that, when weighed at 1 atmosphere and 23°C, is immersed in 10 g of deionized water, and after 24 hours, at least 0.5 g of the immersed polymer dissolves in water. On the other hand, "water-insoluble polymer" refers to a polymer that, when weighed at 1 atmosphere and 23°C, is immersed in 10 g of deionized water, and after 24 hours, at least 0.5 g of the immersed polymer does not dissolve. The water-soluble thermoplastic resin preferably contains one or more selected from substantially non-crosslinked partially saponified polyvinyl alcohol, water-soluble nylon, polyvinylpyrrolidone, and polyethylene oxide, as it causes relatively little irritation to the skin. Water-insoluble thermoplastic resins are preferable because they cause relatively little skin irritation, and preferably contain one or more selected from polyvinyl acetal, polyester, acrylic resin, polystyrene resin, polyvinyl butyral (PVB) resin, polyurethane resin, polyamide resin, polyimide resin, polyamide-imide resin, olefin resin, fully saponified polyvinyl alcohol, and crosslinked partially saponified polyvinyl alcohol. Substantially, "non-crosslinked" encompasses both cases: those that are not crosslinked, i.e., those manufactured without the use of crosslinking agents, and those that are crosslinked under conditions that satisfy the definition of a water-soluble polymer as described above. The term "crosslinking agent" refers to a crosslinking agent that is reactive with hydroxyl groups, and examples include monoaldehydes such as formaldehyde, urea, melamine-formaldehyde resin, boric acid, dialdehyde, diacid, urethane, epoxide, and the like. The polyvinyl acetal preferably contains polyvinyl acetal diethylaminoacetate. The polyester preferably contains one or more selected from polylactic acid, polyethylene terephthalate resin, and polybutylene terephthalate resin. The acrylic resin preferably contains one or more selected from polyacrylonitrile resin and polymethacrylic acid resin. The olefin resin preferably contains one or more selected from polypropylene (PP) and polyethylene. In particular, from the viewpoint of being used in contact with the skin for a long period of time, it is more preferable that the fibers include one or more selected from PVB resin and PP. The present invention also includes cases in which the fibers are primarily composed of any of the preferred synthetic resins (specifically, each preferred resin group) described herein. For example, this includes cases in which the fibers are primarily composed of one or more selected from the preferred water-soluble synthetic resins, or of one or more selected from the preferred (or more preferred) insoluble synthetic resins. These thermoplastic resins can be used individually or in combination of two or more types.

[0021] The porosity of the aforementioned deposit is preferably 60% or more, more preferably 70% or more, and more preferably 75% or more, from the viewpoint of durability and other factors. Furthermore, the porosity of the deposit is preferably 90% or less, and more preferably 85% or less, from the viewpoint of making the deposit less noticeable on the skin. The porosity is preferably in the range of 60% to 90%, more preferably 70% to 90%, and even more preferably 75% to 85%. The deposits in each of the examples described later had a porosity within the range of 75% to 85%. The porosity is determined by the mercury intrusion method. Specifically, it is determined by the following method. (Method for measuring void ratio) Porosity is measured according to the measurement method (mercury intrusion method) described in "Functionality of Materials (Experimental Chemistry Lecture Series 12, 4th Edition), edited by the Chemical Society of Japan, published by Maruzen Co., Ltd., p. 486)," etc. Specifically, porosity can be measured using a dedicated measuring instrument such as a mercury intrusion type pore distribution analyzer (Pore Sizer 9320) manufactured by Shimadzu Corporation. The volume of the sediment is measured in advance, and the pore size distribution of the sediment is measured using the mercury intrusion method. The pore size is calculated using the following formula by the mercury intrusion method. D = -4γcosθ / P However, in the formula, D represents the pore size, γ represents the surface tension of mercury, θ represents the contact angle, and P represents the pressure. The surface tension of mercury is assumed to be 482.536 dyn / cm, the contact angle used is 130°, and measurements are taken at mercury pressures of 0 to 30000 psia. The pore size distribution is determined as follows: First, based on the principle of the mercury intrusion method, the pressure applied to the mercury is gradually changed. At that time, the volume of mercury that enters the pore, i.e., the pore volume dV, is measured. The relationship between the pore size D, converted according to the above formula, and the pore volume dV is plotted. The derivative of this relationship curve, dV / d(logD), is calculated and plotted on the vertical axis, with the pore size D on the horizontal axis, to create a graph. The pore size distribution is measured in the range of pore sizes from 6 nm to 10000 nm. Note that the unit of pore volume, (mL), indicates the amount of mercury that enters the pore, and (g) indicates the mass of the sediment sample used for measurement. At this point, dV / d(logD) is accumulated to find V. Divide this by the previously determined deposition V1, and V / V1 is the void ratio.

[0022] The fibers preferably contain a thermoplastic resin with a glass transition temperature (Tg) of 150°C or lower, more preferably 120°C or lower, and even more preferably 100°C or lower. Keeping the temperature below this upper limit makes the thermoplastic resin easier to process. The fibers preferably contain a thermoplastic resin with a glass transition temperature (Tg) of 45°C or higher, and more preferably a thermoplastic resin with a glass transition temperature (Tg) of 50°C or higher. Setting the temperature above this lower limit improves the handling properties of the thermoplastic resin and the temperature stability of the deposits. In particular, when the fibers contain an amorphous resin, the glass transition temperature of the amorphous resin is preferably 150°C or lower, more preferably 120°C or lower, and especially preferably 100°C or lower. Furthermore, the glass transition temperature of the amorphous resin is preferably 45°C or higher, and more preferably 50°C or higher. Examples of amorphous resins include PVB, polyvinyl alcohol, acrylic resin, polystyrene resin, polyvinyl acetal, and polyamide-imide resin.

[0023] For measuring the Tg of thermoplastic resins, a PerkinElmer differential scanning calorimetry system, "PYRIS Diamond DSC," is used. First, approximately 5 mg of sample is taken from the fiber. Under a nitrogen atmosphere, the sample is sealed in an aluminum closed cell, and then the cell is set in the instrument holder. This is then heated from 25°C to 300°C at a rate of 10°C / min. The DSC curve is obtained by measuring the heat flow rate at each temperature. In the obtained DSC curve, the "intermediate glass transition temperature (Tmg)" described in JIS K 7121 is considered to be the glass transition temperature.

[0024] In this specification, it is preferable that the deposit used for collecting skin surface material is provided onto the skin from an electrostatic spray device, or is in the form of a single layer sheet, or constitutes a laminated sheet laminated on a substrate.

[0025] The method of providing the material onto the skin from the electrostatic spray device can be any of the methods described later in "Method of spraying and depositing fibers onto the skin using a spray device to form a coating consisting of fiber deposits" and the method described later in (I-1) without limitation.

[0026] In the case of a single-layer sheet or a laminated sheet formed by laminating a substrate, the explanation in "Method for attaching a sheet-like deposit of fibers to the skin" and the explanation in (I-2) below can be adopted without limitation.

[0027] As the base material, a breathable material can be used, such as a mesh sheet, a fiber sheet, or a laminate thereof. Examples of fiber sheets include nonwoven fabrics, woven fabrics, knitted fabrics, and paper.

[0028] When applying fiber deposits to the skin, two methods are typically preferred: The first is "a method of spraying fibers onto the skin using a spray device to deposit them and form a film consisting of fiber deposits." The second is "a method of attaching sheet-like fiber deposits to the skin." The deposits used in this invention adhere to the skin due to capillary action resulting from the small diameter of their constituent fibers. This capillary action allows for minimally invasive application, low burden on the skin, and long-term adhesion. Furthermore, there is no need to attach the deposits to the skin using adhesive materials such as adhesive tapes or patches. Examples of fiber deposits in sheet form include the single-layer sheets and fiber deposits in laminated sheets mentioned above.

[0029] The attachment process is not limited to any process that can form a film consisting of the aforementioned fiber deposits on the skin, but it is preferable to include the following steps (I-1) or (I-2). (I-1) A step of electrostatically spraying composition X containing the following volatile substances and polymers onto the skin. The aforementioned volatile substance includes one or more volatile substances selected from water, alcohol, and ketones (hereinafter also referred to as "volatile substance A"). The polymer includes a polymer having the ability to form fibers (hereinafter also simply referred to as "polymer" or "the polymer"). (I-2) A step of applying a deposit of fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less, obtained by electrospinning or melt blowing, to the skin.

[0030] First, the means (I-1) will be explained. The method for forming the deposit in means (I-1) typically involves electrostatic spraying. Electrostatic spraying involves applying a positive or negative high voltage to a composition to charge it, and then spraying the charged composition toward an object. The sprayed composition spreads through space, repeatedly becoming finer due to Coulomb repulsion, and during this process, or after adhering to the object, the volatile solvent dries, forming a film containing fibrous deposits on the surface of the object. This method (I-1) can be carried out using the methods and apparatus described in, for example, WO2018 / 194143

[0007]

[0012]

[0089] , WO2018 / 194140

[0007]

[0012]

[0067] , WO2019 / 103974

[0002]

[0003]

[0004]

[0142]

[0143]

[0144] , etc.

[0031] The volatile substance is typically a substance that is volatile in a liquid state. In composition X, the volatile substance is discharged from the nozzle tip toward the skin after composition X has been sufficiently charged by being placed in an electric field. As the volatile substance evaporates, the charge density of composition X becomes excessive, and the volatile substance evaporates further while composition X is further atomized by Coulomb repulsion. Finally, a dry film consisting of a deposit of fibers is formed on the skin. For this purpose, the vapor pressure of the volatile substance is preferably 0.01 kPa or more and 106.66 kPa or less at 20°C, more preferably 0.13 kPa or more and 66.66 kPa or less, even more preferably 0.67 kPa or more and 40.00 kPa or less, and even more preferably 1.33 kPa or more and 40.00 kPa or less.

[0032] The volatile substance preferably includes one or more selected from monohydric chain aliphatic alcohols, monohydric cyclic aliphatic alcohols, and monohydric aromatic alcohols. The monohydric chain aliphatic alcohol preferably contains one or more selected from C1 to C6 alcohols. The monohydric cyclic aliphatic alcohol preferably contains one or more selected from C4-C6 cyclic alcohols. The monohydric aromatic alcohol preferably contains one or more selected from benzyl alcohol, phenylethyl alcohol, and the like. The C1-C6 alcohol preferably includes one or more selected from ethanol, isopropyl alcohol, butyl alcohol, n-propanol, and n-pentanol. The monohydric aromatic alcohol preferably includes phenylethyl alcohol.

[0033] The volatile substance preferably includes one or more selected from diC1-C4 alkyl ketones. The diC1-C4 alkyl ketone preferably contains one or more selected from acetone, methyl ethyl ketone, and methyl isobutyl ketone.

[0034] From the viewpoint of continuous use on the skin, the volatile substance more preferably contains one or more selected from ethanol, isopropyl alcohol, butyl alcohol, and water, even more preferably contains one or more selected from ethanol and butyl alcohol, and even more preferably contains ethanol.

[0035] From the viewpoint of fiber-forming properties, the content of volatile substances in composition X is preferably 30% by mass or more, more preferably 55% by mass or more, and even more preferably 60% by mass or more. In particular, the content of volatile substance A in composition X is preferably 30% by mass or more, more preferably 55% by mass or more, and even more preferably 60% by mass or more. Furthermore, from the viewpoint of fiber-forming properties, the content of volatile substances in composition X is preferably 98% by mass or less, more preferably 96% by mass or less, and even more preferably 94% by mass or less. Furthermore, the content of volatile substance A in composition X is preferably 30% by mass or more, more preferably 55% by mass or more, and even more preferably 60% by mass or more. From the above viewpoint, the content of volatile substances in composition X is preferably 30% by mass or more and 98% by mass or less, more preferably 55% by mass or more and 96% by mass or less, and even more preferably 60% by mass or more and 94% by mass or less. Furthermore, the content of volatile substance A in composition X is preferably 30% by mass or more and 98% by mass or less, more preferably 55% by mass or more and 96% by mass or less, and even more preferably 60% by mass or more and 94% by mass or less. Composition X preferably contains one or more selected from oils and polyols. In this case, the content of volatile substances in composition X is preferably 30% by mass or more and 90% by mass or less, more preferably 55% by mass or more and 90% by mass or less, and even more preferably 60% by mass or more and 90% by mass or less. Furthermore, the content of volatile substance A in composition X is preferably 30% by mass or more and 90% by mass or less, more preferably 55% by mass or more and 90% by mass or less, and even more preferably 60% by mass or more and 90% by mass or less. By including volatile substances in composition X in these proportions, the volatility of the volatile substances is improved when the electrostatic spray method is performed. If composition X contains ethanol, the amount of ethanol is preferably 50% by mass or more and 100% by mass or less, more preferably 65% ​​by mass or more and 100% by mass or less, and even more preferably 80% by mass or more and 100% by mass or less, relative to the total amount of volatile substances. Furthermore, the amount of ethanol is preferably 50% by mass or more and 100% by mass or less, more preferably 65% ​​by mass or more and 100% by mass or less, and even more preferably 80% by mass or more and 100% by mass or less, relative to the total amount of volatile substance A.

[0036] Polymers having fiber-forming ability are typically substances that can dissolve in the aforementioned volatile substances. Here, dissolution means that the substance is dispersed at 20°C and that the dispersion is uniform to the naked eye, preferably transparent or translucent to the naked eye. Examples of polymers having fiber-forming ability include the aforementioned thermoplastic resins, and preferred examples include preferred resins among thermoplastic resins.

[0037] The polymer content in composition X is preferably 2% by mass or more and 50% by mass or less, more preferably 4% by mass or more and 45% by mass or less, and even more preferably 6% by mass or more and 40% by mass or less. By incorporating the polymer into composition X in this proportion, a film consisting of fiber deposits is formed, and the skin protection effect is achieved by this film.

[0038] The ratio of the volatile substance to the polymer content in composition X (volatile substance / polymer) is preferably 0.5 to 40, more preferably 1 to 30, and even more preferably 1.3 to 25, from the viewpoint of ensuring sufficient volatilization of the volatile substance when performing the electrostatic spray method.

[0039] Composition X may contain glycol. The glycol may contain one or more selected from ethylene glycol, propylene glycol, butylene glycol, diethylene glycol, dipropylene glycol, polyethylene glycol, and polypropylene glycol, etc. From the viewpoint of ensuring sufficient volatilization of volatile substances when performing the electrostatic spraying method, the glycol content in composition X is preferably 0% by mass or more and 10% by mass or less, and more preferably 8% by mass or less. If composition X contains water, from the viewpoint of fiber-forming properties and conductivity, the water content is preferably more than 0% by mass and less than 50% by mass, more preferably 45% by mass or less, even more preferably 10% by mass or less, and even more preferably 5% by mass or less, relative to the total amount of volatile substances. For similar reasons, the water content is preferably more than 0% by mass and less than 50% by mass, more preferably 45% by mass or less, even more preferably 10% by mass or less, and even more preferably 5% by mass or less, relative to the total amount of volatile substance A.

[0040] When performing the electrostatic spraying method, it is preferable to use a composition X whose viscosity at 25°C is preferably 1 mPa·s to 5000 mPa·s, more preferably 10 mPa·s to 2000 mPa·s, and even more preferably 50 mPa·s to 1500 mPa·s. By using a composition X having a viscosity within this range, a porous coating containing fiber deposits can be successfully formed by the electrostatic spraying method. The viscosity of composition X used in each of the examples described later is in the range of 50 mPa·s to 1500 mPa·s. Viscosity is measured at 25°C using an E-type viscometer. An E-type viscometer manufactured by Tokyo Keiki Co., Ltd. can be used. In this case, rotor No. 43 can be used.

[0041] An electrostatic spray device typically comprises a container 14, a nozzle 15, a supply device, a power supply 12 (high-voltage power supply), a low-voltage power supply 11, and an auxiliary electrical circuit 13. The container 14 contains composition X. The nozzle 15 dispenses composition X. The supply device supplies composition X contained in the container 14 to the nozzle 15. The supply device typically comprises a microgear pump 15A, a conduit 15B, and a flexible conduit 15C. The power supply 12 applies voltage to the nozzle 15. The low-voltage power supply 11 typically consists of one or two batteries. The auxiliary electrical circuit 13 regulates the voltages of the high-voltage power supply 12 and the low-voltage power supply 11. In addition, the auxiliary electrical circuit 13 controls the rotational speed of the motor provided in the microgear pump 15A. Figure 1 shows a schematic diagram illustrating the configuration of an electrostatic spray device preferably used in the present invention. Figure 2 shows the process of electrostatic spraying onto one's own skin.

[0042] Next, I will explain the means (I-2). Method (I-2) is a step of applying a deposit of fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less, obtained by electrospinning or melt blowing, to the skin. An example of applying the fiber deposit to the skin in method (I-2) is a method of attaching a pre-formed film-like fiber deposit to the skin. The deposit of fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less used in this method (I-2) is preferably obtained by electrospinning or melt-blowing on a substrate using the composition X. The electrospinning method can be carried out in the same manner as the electrostatic spraying method. However, the voltage during electrospinning may be high since it is not performed on the skin. Examples of substrates include metals, resins, and the various substrate films mentioned above. The melt-blowing method melts the resin at a temperature above its melting point and discharges it while also discharging hot air around the discharge port of the molten resin to form a deposit of fibers with the aforementioned average fiber diameter.

[0043] While not limited to this, when applying a fibrous deposit to the skin, the outer surface area of ​​the skin portion in contact with the deposit during a single application should be, for example, 0.5 cm². 2 More than 400cm 2 The following is preferable: 0.7 cm 2 More than 225cm 2 The following is more preferable: When applying the fibrous deposit to the skin, it may be applied separately to multiple locations on the skin, or it may be applied continuously to one location.

[0044] When employing method (I-1) or (I-2), it is preferable to apply liquid agent Y to the skin either “before the adhesion process” or to the deposit “during the adhesion process.” Liquid agent Y preferably contains one or more components selected from water, polyol, and an oil that is liquid at 20°C. This can impart good transparency to the deposit formed by the adhesion process, thereby improving its appearance. It can also enhance the adhesion between the skin and the deposit, making it easier for the skin surface material to adhere to the deposit. An example of a "pre-adhesion process" is before applying the deposit to the skin. An example of the "adhesion process" (while the skin surface material is adhering to the deposit) is the period after the deposit is applied to the skin. In this case, it is preferable to apply the deposit to the skin, then apply the liquid agent Y, and then perform the preferred retention time described above. In this invention, when the deposit is used in combination with liquid agent Y, even if the skin is covered with the deposit during daily life, there is little change in appearance, and it is easy to apply for long periods of time.

[0045] Liquid Y preferably contains water and polar oil, as this improves the adhesion of the deposit to the skin. The total proportion of water and polar oil in liquid Y is preferably 40% by mass or more and 100% by mass or less. The polar oil preferably includes an ester oil, as described later.

[0046] The polyol preferably contains one or more selected from alkylene glycols, polyalkylene glycols, and glycerols. The alkylene glycols preferably contain one or more selected from ethylene glycol, propylene glycol, 1,3-propanediol, and 1,3-butanediol. The polyalkylene glycols preferably contain one or more selected from diethylene glycol, dipropylene glycol, polyethylene glycol with a molecular weight of 1000 or less, and polypropylene glycol, etc. The glycerin compounds preferably include one or more selected from glycerin, diglycerin, and triglycerin. Of these, from the viewpoint of excellent adhesion to the skin, abrasion resistance, stretchability, and transparency of the coating, it is preferable to include one or more selected from ethylene glycol, propylene glycol, 1,3-butanediol, dipropylene glycol, polyethylene glycol with a molecular weight of 1000 or less, glycerin, and diglycerin. It is even more preferable to include one or more selected from propylene glycol, 1,3-butanediol, and glycerin, and even more preferable to include glycerin.

[0047] The polyol content in liquid Y is preferably 1% to 40% by mass, more preferably 1% to 30% by mass, even more preferably 3% to 25% by mass, even more preferably 5% to 20% by mass, and particularly preferably 10% to 20% by mass, from the viewpoint of excellent abrasion resistance, stretchability, and transparency of the coating.

[0048] Liquid agent Y preferably contains a liquid oil at 20°C, from the viewpoint of excellent abrasion resistance, stretchability, and transparency. The liquid oil at 20°C also includes a fluid semi-solid. The liquid oil at 20°C preferably contains one or more selected from hydrocarbon oils, ester oils, higher alcohols, silicone oils, and fatty acids. Of these, it is preferable to include one or more selected from hydrocarbon oils, ester oils, and silicone oils from the viewpoint of smoothness during application, abrasion resistance of the film, and extensibility.

[0049] The hydrocarbon oil preferably contains one or more selected from liquid paraffin, squalane, squalene, n-octane, n-heptane, cyclohexane, light isoparaffin, liquid isoparaffin, hydrogenated polyisobutene, polybutene, polyisobutene, hydrogenated polydecene, and α-olefin oligomers. In particular, from the viewpoint of usability, it is preferable to contain one or more selected from liquid paraffin, light isoparaffin, liquid isoparaffin, squalane, squalene, n-octane, n-heptane, cyclohexane, hydrogenated polydecene, and α-olefin oligomers.

[0050] The ester oil preferably contains an ester consisting of a straight-chain or branched-chain fatty acid and a straight-chain or branched-chain alcohol or polyhydric alcohol. Such esters include isopropyl myristate, cetyl isooctanoate, isocetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, decyl oleate, octyldodecyl oleate, hexyldecyl dimethyloctanoate, cetyl lactate, myristyl lactate, lanolin acetate, isocetyl stearate, isocetyl isostearate, ethylhexyl isononanoate, isononyl isononanoate, isotridecyl isononanoate, isostearyl isostearate, and 12-hydroxystearyl Cholesteryl ethyl acid, ethylene glycol di-2-ethylhexanoate, dipentaerythritol fatty acid ester, N-alkyl glycol monoisostearate, propylene glycol dicaprylate, propylene glycol diisostearate, neopentyl glycol dicaprate, diisostearyl malate, glyceryl di-2-heptylundecanoate, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, glyceryl tri-2-ethylhexanoate, triisos Trimethylolpropane thearate, cetyl 2-ethylhexanoate, 2-ethylhexyl palmitate, diethylhexyl naphthalenedicarboxylate, alkyl benzoate (C12-C15), cetearyl isononanoate, caprylic / capric triglyceride, dicaprylic / capric butylene glycol, dicaprylic / capric propylene glycol, glyceryl triisostearate, glyceryl tri-2-heptyl undecanoate, glyceryl coconut oil fatty acid ester, methyl castor oil fatty acid ester, oleyl oleate, palmitate It is preferable to include one or more selected from 2-heptyl undecyl tinate, diisobutyl adipate, 2-octyldodecyl N-lauroyl-L-glutamic acid, di2-heptyl undecyl adipate, ethyl laurate, di2-ethylhexyl sebacate, 2-hexyldecyl myristate, 2-hexyldecyl palmitate, 2-hexyldecyl adipate, diisopropyl sebacate, di2-ethylhexyl succinate, triethyl citrate, 2-ethylhexyl paramethoxycinnamate, and tripropylene glycol dipivalate. Among these, it is preferable to include one or more selected from octyldodecyl myristate, myristyl myristate, isocetyl stearate, isononyl isononanoate, isocetyl isostearate, cetearyl isononanoate, diisobutyl adipate, di-2-ethylhexyl sebacate, isopropyl myristate, isopropyl palmitate, diisostearyl malate, neopentyl glycol dicaprate, and tri(caprylic / capric acid)glycerin, from the viewpoint of excellent abrasion resistance, stretchability, and transparency.

[0051] The ester oil may contain one or more selected from vegetable oils and animal oils. The vegetable oil may include one or more selected from olive oil, jojoba oil, macadamia nut oil, meadowfoam oil, castor oil, safflower oil, sunflower oil, avocado oil, canola oil, apricot kernel oil, rice germ oil, and rice bran oil.

[0052] The aforementioned higher alcohol preferably contains one or more liquid higher alcohols having 12 to 20 carbon atoms. The liquid higher alcohol having 12 to 20 carbon atoms preferably contains one or more selected higher alcohols having branched fatty acids as components, and more preferably contains one or more selected from isostearyl alcohol, oleyl alcohol, and cetanol, etc.

[0053] The silicone oil preferably contains one or more selected from linear silicones, cyclic silicones, and modified silicones, and more preferably contains one or more selected from dimethylpolysiloxane, dimethylcyclopolysiloxane, methylphenylpolysiloxane, methylhydrogenpolysiloxane, phenyl-modified silicones, and higher alcohol-modified organopolysiloxanes.

[0054] The content of the liquid oil in liquid Y, which is liquid at 20°C, is preferably 1% by mass or more and 20% by mass or less, more preferably 2% by mass or more and 18% by mass or less, and even more preferably 3% by mass or more and 16% by mass or less, from the viewpoint of excellent adhesion to the skin, abrasion resistance, stretchability and transparency of the coating.

[0055] The step of applying liquid Y to the skin may occur before or during the adhesion step, specifically while or after the application of the deposit to the skin. Means for applying liquid Y to the skin include using an applicator. Note that composition X and liquid Y have different compositions.

[0056] The fiber deposits and liquid agent Y used in this invention may be adhesive-free. Here, adhesive refers to a component that exhibits adhesiveness on its own, and examples include various adhesives such as natural rubber-based, synthetic rubber-based, acrylic-based, silicone-based, and hot-melt-based adhesives. The absence of adhesive in the fiber deposit means that the adhesive content in the fiber deposit is 0% by mass or more and 5% by mass or less, and preferably 3% by mass or less. In liquid formulation Y, the absence of an adhesive means that the adhesive content in liquid formulation Y is 0% by mass or more and 1% by mass or less, and preferably 0.5% by mass or less.

[0057] The adhesion process of the present invention typically involves applying a deposit containing fibers with an average fiber diameter of 0.01 μm to 7 μm onto the skin, and then holding the deposit in place on the skin. From the viewpoint of allowing more surface skin cells from the skin to adhere to the deposit, the retention time of the deposit is preferably 0.5 hours or more, more preferably 2 hours or more, even more preferably 3 hours or more, and even more preferably 4 hours or more. Since the amount of surface material in the sediment does not increase significantly even if the retention time is extended beyond a certain point, a retention time of 12 hours or less is practical.

[0058] After retaining the deposit on the skin for a predetermined period of time, the film is typically removed from the skin. The removal method is preferably done by wearing gloves and peeling the deposit off the skin with tweezers or similar tools. The removed deposit is then collected. This step allows for the collection of the deposit along with any skin surface material that had adhered to it during the retention period.

[0059] The recovered fiber deposits preferably have hydrophilic and hydrophobic components attached as surface materials. Examples of hydrophilic components include proteins, nucleic acids, sugars, and amino acids. These may or may not be metabolites. Hydrophobic components include sebum (lipids derived from sebaceous glands) and lipids derived from keratinocytes. Lipids include triglycerides, diglycerides, monoglycerides, free fatty acids, squalene, waxes, and cholesterol. Albumin, an immune response-related protein, is a hydrophilic component, while keratin, a structural protein of the stratum corneum, is a hydrophobic component.

[0060] One method for analyzing sediment after the removal process is to examine the amount and / or type of compounds constituting the surface material contained in the collected sediment. The examination of the amount and / or type of the aforementioned compound preferably includes one or more methods selected from genome analysis, epigenetics (e.g., methylation), transcriptome analysis (RNA expression), proteome analysis (proteomics), etc. Genomic analysis can be performed, for example, by a DNA sequencer. Methylation analysis can be performed, for example, by methylated base conversion followed by DNA sequencing. RNA expression analysis can be performed, for example, by RT-PCR analysis or RNA-seq analysis. Proteomics analysis can be performed, for example, by mass spectrometry (e.g., tandem mass spectrometry (MS / MS) or by methods using LC-MS / MS, which combines MS / MS with liquid chromatography (LC) (e.g., sequence tagging). Multi-omics analysis, which analyzes these simultaneously, may also be employed. Proteome analysis includes the analysis of post-translational modifications. Analysis of post-translational modifications includes the analysis of phosphorylation, methylation, glycosylation (including glycosylation), ubiquitination, nitrosylation, methylation, acetylation, lipidation, and proteolysis.

[0061] Analysis may be performed using methods other than those described above. For example, for protein analysis, the Edman method or a peptide sequencer or protein sequencer that automates these methods may be used, or a method that detects proteins using antigen-antibody reactions of specific proteins may be employed. Furthermore, various component analyses can be used as part of sebum analysis.

[0062] In particular, in the present invention, it is preferable to include the quantity and / or type of protein as the target of analysis, in order to take advantage of the excellent capture ability of the capillary force of the deposits according to the present invention for proteins that have seeped from inside the skin to the surface. Examples of protein types include those listed above. In particular, in the present invention, it is more preferable to include the quantity and / or type of immune response-related proteins as the target of analysis.

[0063] In the present invention, the sediment recovered by the above collection method (sediment removed from the skin) may be directly subjected to analysis of the amount and / or types of compounds constituting the skin surface material. Alternatively, the skin surface material may be separated from the sediment before being subjected to analysis of the amount and / or types of compounds constituting the skin surface material. From the viewpoint of applicability to various analytical methods, it is preferable to separate the skin surface material from the recovered sediment. When separating surface material from recovered sediment, it is preferable to dissolve the components to be analyzed in the surface material in a test solution capable of dissolving them. Any liquid used in the art can be used as the test solution. The test solution is often an aqueous solution. In this specification, an aqueous solution means a liquid containing more than 50% by mass of water, for example, 60% by mass or more is preferred, 70% by mass or more is more preferred, 80% by mass or more is even more preferred, and 90% by mass or more is particularly preferred. As the test solution, for example, an aqueous solution containing various inorganic salts, sugars, surfactants, amino acids, etc. may be used. Extracts from commercially available proteome extraction kits may also be used.

[0064] When performing mass spectrometry on proteins, proteins are generally broken down with proteolytic enzymes. However, to suppress autolysis and improve the efficiency of the breakdown, the proteins are usually denatured by heat before enzymatic degradation. First, the sediment containing thermoplastic resin fibers is immersed in the testing solution. Then, the proteins attached to the sediment are dissolved in the testing solution, and the sediment, along with the testing solution, is heated to a temperature above the protein denaturation temperature. In this case, it is easy to set the heating temperature above the glass transition temperature of the thermoplastic resin contained in the sediment. Therefore, heating at this temperature causes the film-like sediment to shrink, making it easier to remove only the sediment from the testing solution. Thus, heating the recovered sediment before protein mass spectrometry allows for simultaneous protein denaturation and deformation of the sediment, which is necessary for mass spectrometry, thus improving work efficiency and making it preferable. From the viewpoint of maintaining a temperature above the protein denaturation temperature and facilitating deformation of the thermoplastic resin, a heating temperature of 60°C or higher is preferable, 70°C or higher is more preferable, and 80°C or higher is particularly preferable. As described above, the heating temperature of the deposit is preferably above the Tg of the thermoplastic resin, from the viewpoint of shrinking the film and improving its handling properties. For example, if the thermoplastic resin is PVB, the glass transition temperature Tg is usually 65°C or higher and less than 70°C, so the heating temperature is preferably 70°C or higher, and more preferably 80°C or higher. For example, if the thermoplastic resin is PP, the heating temperature is preferably 60°C or higher, and more preferably 70°C or higher, due to its ease of deformation. Furthermore, from the viewpoint of preventing excessive denaturation such as protein degradation, the heating temperature is preferably 160°C or lower, more preferably 130°C or lower, and particularly preferably 100°C or lower. When heating to a temperature (Tg) or higher, the heating time is preferably 5 seconds or more, from the viewpoint of improving handling. Furthermore, when heating above Tg, the heating time is practically limited to 120 minutes or less.

[0065] The present invention's method for examining skin surface materials is preferably applied to continuously collecting skin surface materials from a subject's skin at predetermined intervals, examining the collected materials, and accumulating the analysis results. This allows the minimally invasive and simple nature of the present invention's examination method to be utilized. Furthermore, it enables daily monitoring of the subject's health status in a way that places minimal physical burden on the subject. The present invention's method for inspecting skin surface substances involves covering the skin with a deposit of fibers, thereby preventing unconsciously touching the face or other areas in daily life and wiping away substances originating from the body, as well as rubbing against clothing or masks and wiping away such substances. This is particularly effective in accumulating trace amounts of substances originating from the body that are released gradually over a long period of time.

[0066] The method for collecting skin material of the present invention may be applied to the skin of mammals other than humans. It is preferable to apply it to humans because it eliminates the need for pretreatment such as hair removal and is inconspicuous when applied to the skin. Furthermore, it is preferable because it offers the cosmetic benefits previously associated with fibrous deposits, thus demonstrating high potential for industrial application. The person applying the fibrous deposit to the skin and collecting the deposit may be the same person as the person from whom the skin sample is collected, or a different person. For example, the same person may use this collection method and examination method as their own diagnostic tool. An example of a different person would be a nurse, caregiver, etc., applying the fibrous deposit to the skin and collecting the deposit when they want to measure the health status of a subject.

[0067] Along with the embodiments described above, this specification discloses the following: [1] The process involves applying a deposit containing fibers with an average fiber diameter of 0.01 μm to 7 μm to the skin, holding it for 0.5 hours or more, and allowing skin surface substances, which are internally derived substances present on the stratum corneum of the skin, to adhere to the deposit. The process includes a removal step for removing the deposits that have undergone the adhesion step from the skin, A method for collecting surface material, which involves collecting the deposit after a removal process to which surface material is attached. [2] The method for collecting a skin surface substance according to [1], wherein the aforementioned fibers contain a thermoplastic resin. [3] The basis weight of the aforementioned sediment is 0.1 g / m². 2 More than 50g / m 2 The method for collecting skin surface material described in [1] or [2] below. [4] A method for collecting skin surface material according to any one of items [1] to [3], wherein a liquid containing one or more selected from water, polyol, and oils that are liquid at 20°C is applied to the skin before or during the adhesion process. [5] A method for collecting a skin surface substance according to any one of items [1] to [4], wherein the skin surface substance contains a hydrophilic component and a hydrophobic component. [6] A method for collecting skin surface material as described in any one of items [1] to [5], wherein the attachment process involves performing a step selected from (I-1) and (I-2) below. (I-1) A step of electrostatically spraying onto the skin a composition containing one or more volatile substances selected from water, alcohol, and ketones, and a polymer having fiber-forming ability. (I-2) A step of applying a deposit of fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less, obtained by electrospinning or melt blowing, to the skin. [7] The method for collecting a skin surface substance according to any one of items [1] to [6], wherein the skin surface substance comprises one or more selected from proteins, nucleic acids, and lipids. [8] The protein comprises one or more selected from immune response proteins, natural moisturizing factor-producing enzymes, ceramide metabolic enzymes, stratum corneum exfoliating proteases, oxidoreductases, desmosome constituent proteins, and plasma-derived proteins. The nucleic acid includes DNA and / or RNA. The method for collecting skin surface material according to [7], wherein the lipids include lipids derived from sebaceous glands and / or lipids derived from keratinocytes. [9] The method for collecting skin surface material according to any one of [1] to [8], wherein the volatile substance comprises one or more selected from ethanol, isopropyl alcohol, butyl alcohol, and water, preferably comprising one or more selected from ethanol and butyl alcohol, and more preferably comprising ethanol.

[10] The method for collecting skin surface material according to [6], wherein the polymer is soluble in the volatile substance.

[11] The method for collecting skin surface material according to any one of items [1] to

[10] , wherein the skin to which the deposit is applied is one or more locations selected from the face, neck, hands, feet, back, shoulders, abdomen, chest, legs, and arms.

[12] The method for collecting surface material of the skin according to any one of items [1] to

[11] , wherein the average fiber diameter of the fibers contained in the sediment is 0.05 μm or more and 5 μm or less, preferably 0.1 μm or more and 3 μm or less.

[13] The basis weight of the aforementioned sediment is 0.4 g / m². 2 More than 40g / m 2 Preferably 0.5 g / m 2 More than 30g / m 2 More preferably, 0.8 g / m 2 More than 10g / m2 More preferably 1 g / m 2 More than 10g / m 2 The method for collecting skin surface material described in any one of the following items [1] to

[12] .

[14] The method for collecting skin surface material according to any one of items [1] to

[13] , wherein the aforementioned fiber mainly contains synthetic resin.

[15] The aforementioned synthetic resin includes a water-soluble or water-insoluble thermoplastic resin. The water-soluble thermoplastic resin preferably comprises one or more selected from substantially non-crosslinked partially saponified polyvinyl alcohol, water-soluble nylon, polyvinylpyrrolidone, and polyethylene oxide. The method for collecting skin surface material according to

[14] , wherein the water-insoluble thermoplastic resin preferably comprises one or more selected from polyvinyl acetal, polyester, acrylic resin, polystyrene resin, polyvinyl butyral resin, polyurethane resin, polyamide resin, polyimide resin, polyamide, polyamideimide resin, olefin resin, fully saponified polyvinyl alcohol, and crosslinked partially saponified polyvinyl alcohol.

[16] The method for collecting skin surface material according to any one of [1] to

[15] , wherein the fiber comprises a thermoplastic resin having a glass transition temperature Tg of 45°C or more and 150°C or less, preferably a thermoplastic resin having a glass transition temperature Tg of 50°C or more and 120°C or less, and more preferably a thermoplastic resin having a glass transition temperature Tg of 50°C or more and 100°C or less.

[17] A method for collecting skin surface material according to any one of items [1] to

[16] , wherein the aforementioned deposit is applied to the skin without using an adhesive.

[18] A method for collecting surface material according to any one of items [1] to

[17] , wherein the thickness of the deposit is 1 μm or more and 300 μm or less, preferably 1 μm or more and 100 μm or less, more preferably 1 μm or more and 20 μm or less, and particularly preferably 3 μm or more and 20 μm or less.

[19] A method for collecting skin surface material described in any one of items [1] to

[18] , which is non-invasive to the skin.

[20] A method for collecting skin surface material according to any one of [1] to

[19] , wherein the time for which the deposit is retained on the skin is 2 hours or more and 12 hours or less, preferably 3 hours or more and 12 hours or less, more preferably 4 hours or more and 12 hours or less. 〔twenty one〕 A method for collecting surface material according to any one of [1] to

[20] , comprising the step of separating surface material from the recovered sediment. 〔twenty two〕 A method for examining skin surface material obtained by the collection method described in any one of items [1] to

[21] , A method for inspecting surface material, comprising the step of separating surface material from the recovered sediment. 〔twenty three〕 The method for examining skin surface material according to

[22] , wherein the skin surface material is separated from the deposit by immersing the deposit in an examination solution and extracting the skin surface material. 〔twenty four〕 The constituent fibers of the aforementioned deposit include a thermoplastic resin. A method for inspecting a skin surface according to

[22] or

[23] , comprising the step of heating the deposit at a temperature between the glass transition temperature of the thermoplastic resin and 160°C, preferably between 60°C and 100°C. 〔twenty five〕 The constituent fibers of the aforementioned deposit include a thermoplastic resin. A method for inspecting a skin surface according to any one of

[22] to

[24] , wherein the thermoplastic resin contains polyvinyl butyral, and the temperature at which the deposit is heated is 70°C or more and 100°C or less, preferably 80°C or more and 100°C or less.

[26] The constituent fibers of the aforementioned deposit include a thermoplastic resin. A method for inspecting a skin surface according to any one of

[22] to

[25] , wherein the thermoplastic resin contains polypropylene, and the temperature at which the deposit is heated is 60°C or more and 100°C or less, preferably 70°C or more and 100°C or less.

[27] The method for inspecting a surface material of the skin according to any one of items

[22] to

[26] , wherein the time for heating the aforementioned deposit is 5 seconds or more and 120 minutes or less.

[28] A method for examining a skin surface according to any one of paragraphs

[22] to

[27] , for examining the amount and / or type of compounds contained in the skin surface.

[29] The method for examining a skin surface according to

[28] , wherein the examination of the amount and / or type of the compound comprises one or more selected from genomic analysis, epigenetics, transcriptome analysis and proteome analysis.

[30] The method for examining a skin surface according to

[28] or

[29] , which includes the analysis of proteins, for examining the amount and / or type of the aforementioned compound.

[31] A method for monitoring health status, which uses a skin sample obtained by the collection method described in any one of items [1] to

[21] , or uses the examination method described in any one of items

[22] to

[30] , A method for monitoring health status, which involves continuously collecting skin surface material from a subject's skin at predetermined intervals, examining the collected material, and accumulating the analysis results.

[32] A method for monitoring health conditions as described in

[31] , wherein the subject of monitoring is a human being.

[33] A fibrous deposit used to collect epidermal substances, which are endogenous substances present on the stratum corneum of the skin, Sediments containing fibers with an average fiber diameter of 0.01 μm to 7 μm.

[34] The deposit according to

[33] , wherein the deposit is a single layer sheet, is laminated on a substrate to form a laminated sheet, or is provided onto the skin from an electrostatic spray device.

[35] The sediment according to

[33] , wherein the fibers are obtained by electrospinning or meltblowing.

[36] A kit comprising a sediment described in any one of items

[33] to

[35] and a test solution for immersing the sediment.

[37] A test kit comprising the sediment described in any one of items

[33] to

[35] and a liquid agent, The aforementioned test kit is The aforementioned deposit is applied to the skin, By holding it for 0.5 hours or more, the skin surface substances, which are internally derived substances present on the stratum corneum of the skin, adhere to the deposits. Next, the deposit is removed from the skin, and the removed deposit is collected. The liquid agent is applied to the skin before or while the skin surface material is attached to the deposit, The aforementioned liquid preparation contains one or more selected from water, polyol, and oils that are liquid at 20°C. Test kit. [Examples]

[0068] The present invention will be described in more detail below with reference to examples. However, the scope of the present invention is not limited to these examples. Unless otherwise specified, "%" and "parts" mean "mass%" and "parts by mass." In Experimental Example 1, the coating formed by electrostatic spraying was a deposit containing fibers. Its average fiber diameter was 1 μm, and its basis weight was 4 g / m². 2 This has been confirmed. The resin used is PVB with a Tg of 65°C.

[0069] Experimental Example 1 (Examples 1-1 to 1-3, Comparative Examples 1-1 to 1-3) For the skin of one subject (a woman in her 30s), samples were collected from the cheek and forehead. (1) A film consisting of fibrous deposits was formed on the right cheek and right forehead, and (2) an oil-absorbing film was used to collect skin surface material on the left cheek and left forehead. In Figures 3 to 5, film-1 corresponds to the left forehead, film-2 to the left cheek, deposit-1 to the right forehead, and deposit-2 to the right cheek. The samples from the forehead and cheeks were collected on separate days. On day 1, samples were collected from the forehead using deposits and an oil-absorbing film, and on day 2, samples were collected from the cheeks using deposits and an oil-absorbing film. The procedure for Example 1 described below also includes the collection of samples from the forehead and cheeks. (1) For the formation of a coating consisting of fibrous sediments, the following steps (a) to (c) were carried out in succession in this order. (a) A film was formed on the skin at 6:30 and maintained for 6.5 hours until 13:00. (b) A film was formed on the skin at 13:00 and maintained for 7 hours until 20:00. (c) A film was formed on the skin at 8 PM and maintained for 9.5 hours until the following morning. In (a) to (c), after washing the face, a film consisting of fiber-containing deposits was formed on the target area (right forehead or right cheek). The film consisting of fiber-containing deposits was obtained by electrostatic spraying the target area with the composition shown in Table 1 under the following conditions. After electrostatic spraying, it was blended in by lightly pressing with a puff. On the right forehead, the area where the film consisting of fiber-containing deposits was formed covered almost the entire area of ​​half of the forehead. Similarly, on the right cheek, the area where the film consisting of fiber-containing deposits was formed covered almost the entire area of ​​the right cheek. <Conditions for electrostatic spraying> Voltage: 25KV Flow rate: 60mL / min Distance from nozzle to skin: 16cm Spray time: 15 seconds Room temperature 23℃, humidity 35%RH

[0070] The composition of the electrostatic spray is as follows: [Table 1]

[0071] At each of the following points in time, after the holding time in (a) (before (b)), after the holding time in (b) (before (c)), and after the holding time in (c), a film consisting of fibrous deposits was collected. The collector wore gloves, grasped the edge of the film with tweezers, and slowly peeled it off. This operation was repeated until the entire formed film was collected. The collected film was placed in a screw tube and stored in a deep freezer until it was to be used for pretreatment.

[0072] (2) When an oil-absorbing film was used, instead of forming a film consisting of fibrous deposits in (1), after the holding time in (a) to (c) had elapsed, a sample of skin surface material was collected using an oil-absorbing film (5 cm x 8 cm, manufactured by 3M) in the following manner. The sample collector wore gloves. One oil-absorbing film was used to thoroughly rub the target area (left forehead or left cheek) to collect surface skin material. The oil-absorbing film was placed in a screw-top tube and stored in a deep freezer until ready for pretreatment. The area rubbed with the oil-absorbing film on the left cheek and left forehead was equivalent to the area of ​​film formation consisting of fibrous deposits on the right cheek and right forehead, respectively. Furthermore, the subjects of the study were not subject to any restrictions on their activities, including skincare, makeup, and mask-wearing, and lived their lives as usual.

[0073] (Protein extraction (albumin / keratin area ratio, number of identified proteins)) Proteins were extracted from the obtained coatings and oil-absorbing films using the following method. For each of the obtained coatings and oil-absorbing films, the peeled portion from the cheek or forehead for the coatings, and the film used to rub the cheek or forehead for the oil-absorbing films, were placed in 0.3 mL of the test extraction solution (12 mmol / L Sodium deoxycholate, 12 mmol / L Sodium lauroyl sarcosinate, 100 mmol / L Tris-HCl (pH9.0) aqueous solution) and heated at 95°C for 5 minutes. After that, they were sonicated in ice water for 20 minutes. 3000 g was centrifuged at 4°C for 5 minutes, and the supernatant was collected to separate the coatings that had shrunk due to heating. Transfer 0.2 mL of the supernatant to another tube, add 8.7 μL of TCEP (Tris(2-carboxyethyl)phosphine) and 8.7 μL of S-Methyl Methanethiosusponate for reductive alkylation treatment, and then add 27.5% aqueous phosphoric acid solution to a final concentration of 2.5%. Each sample was then treated with S-Trap. TMThe samples were loaded onto micro columns (ProtiFi). After loading, the samples were washed with 90% methanol / 10% 1 mol / L TEAB (Triethylammonium bicarbonate) aqueous solution and 50% chloroform / 50% methanol. Then, trypsin and Lys-C were added, and the samples were enzymatically digested by incubation at 37°C overnight. 40 μL of 50 mmol / L TEAB aqueous solution, 40 μL of 0.2% formic acid aqueous solution, and 40 μL of 50% acetonitrile aqueous solution were sequentially added to elute the digested peptides. The eluates from each stage were pooled together and dried by vacuum centrifugation. After desalting using GL-Tip SDB (GL Sciences Co., Ltd.), the samples were dried by vacuum centrifugation. The samples were kept at -80°C until further analysis.

[0074] The digested peptides described above were dissolved in 30 μL of a 2% acetonitrile aqueous solution containing 0.1% formic acid. The peptide concentration was calculated using liquid chromatography with UV detection (LC-UV, measurement conditions the same as in Experimental Example 2), and the amount of recovered protein was measured.

[0075] Liquid chromatography-mass spectrometry (LC-MS) was used for peptide analysis. The liquid chromatography conditions were as follows: Solvent of sample solution: 2% acetonitrile aqueous solution containing 0.1% formic acid Mobile phase: A) 0.1% formic acid aqueous solution, B) 80% acetonitrile aqueous solution containing 0.1% formic acid Desalination column: Acclaim, manufactured by Thermo Fisher Scientific. PepMap 100 Nano Trap C18 nano Viper (inner diameter 75μm, length 20mm, particle size 3μm) Separation column: Acclaim, manufactured by Thermo Fisher Scientific. PepMap 100 RSLC, C18 (inner diameter 75 μm, length 150 mm, particle size 2 μm) Elution rate: 0.3μL / min Solvent gradient conditions: B 5% (0-5 min) → B 50% (125 min) → B 95% (126-150 min) → auto-calibration B 95% (155 min) → B 5% (156-180 min) Column temperature: 40℃ Peptide injection volume: 0.3 μg Under the above conditions, the peptide was eluted from the column into a mass spectrometer using liquid chromatography.

[0076] The following equipment and conditions were set up for mass spectrometry. Emitter: New Objective Pico Tip NanoSpray Emitter FS360-50-15-N Mass spectrometer: Thermo Fisher Scientific Orbitrap Q Exactive Plus Mode: nano ESI

[0077] Processing and analysis of mass spectrometry data Analysis software: Proteome Discoverer (Thermo Fisher Scientific) Analysis database: Swiss-prot (Homo sapience) (Other settings: Number of disconnection errors allowed: 2, Variable modification: Oxidation (M)) Immobilization modification: Methylchio (C), Digestive enzyme: Trypsin, Search engine: Mascot (Matrix Science), Peptide identification threshold: FDR < 5% Under the above conditions, a database search was performed, and the peak area values ​​of each identified protein were normalized to the total peak area value of all identified proteins. The albumin / keratin-10 ratio was calculated using the normalized peak area values.

[0078] The results are shown in Figures 3-5.

[0079] (a) was designated as Example 1-1 and Comparative Example 1-1, (b) as Example 1-2 and Comparative Example 1-2, and (c) as Example 1-3 and Comparative Example 1-3, and these are summarized in Table 2 below. The long-term adhesion, peelability, and appearance of the samples in Table 2 were evaluated according to the following criteria.

[0080] (long-term application) This indicates the duration of adhesion. When the duration of adhesion is equal to the retention time, it indicates that the patch adheres to the skin for the set retention time. A value of 0 indicates that the patch does not adhere at all.

[0081] (Peelability) The peelability was evaluated by whether or not the sheet tore during peeling, and if so, how many pieces it broke into. A value of 1 indicates that there was only one sheet, no splitting, and the best peelability.

[0082] (exterior) Examples 1-1 to 1-3 involved preparing samples by forming deposits on commercially available nylon film using electrospinning. Comparative Examples 1-1 to 1-3 involved preparing samples by layering and attaching oil-absorbing film to commercially available nylon film. The transmittance (%T) of the prepared samples was measured using a spectrophotometer (U-3310, Hitachi, Ltd., wavelength 600 nm). The measurement was performed by setting the deposit or oil-absorbing film (object) cut to 20 mm x 40 mm in the cell holder of the spectrophotometer so that the light from the light source was perpendicular to the object. A transmittance of 20% or more was evaluated as transparent, and a transmittance of less than 20% was evaluated as opaque.

[0083] [Table 2]

[0084] Figure 3 shows the total peak area of ​​albumin-derived peptides extracted from sediment or oil-absorbing film. Albumin is an example of an immune response protein present in interstitial fluid, and it can be seen that more albumin can be obtained from fibrous sediment than from oil-absorbing film. Furthermore, as shown in Figure 4 and Table 2, the peak area ratio of keratin-10 to albumin (ALB / KRT10) was also higher when forming fibrous sediment than when using oil-absorbing film. Keratin-10 is a protein that makes up the stratum corneum, and is not a surface substance that exists on top of the stratum corneum. Therefore, according to the present invention, it can be seen that albumin, which is a surface substance, can be selectively and non-invasively obtained from stratum corneum constituent proteins such as keratin-10. Figure 5 and Table 2 show the measurement results of the number of identified proteins. It can be seen that a larger number of protein types can be obtained using the present invention compared to when using oil-absorbing film. In other words, according to the present invention, since albumin can be selectively obtained and a large number of types of proteins can be obtained, surface skin materials can be collected efficiently. Furthermore, Table 2 shows that the present invention exhibits excellent long-term adhesion properties and superior appearance when applied, due to the use of a specific fiber deposit.

[0085] Experimental Examples 2 (Examples 2-1 to 2-6, Comparative Examples 2-1, 2-2) Experiment Example 2 was conducted with one different subject (a woman in her 30s) from Experiment Example 1.

[0086] The sample extraction conditions and liquid chromatography conditions were as follows. <Sample extraction conditions> Proteins were extracted from the obtained coatings and oil-absorbing films using the following method. For each of the obtained coatings and oil-absorbing films, the peeled portion from the cheek for the coatings and the film used to rub the cheek for the oil-absorbing films were combined and placed in 0.5 mL of the test extraction solution (5% Sodium n-Dodecyl Sulfate, 1 mol / L TEAB (pH 8.5) aqueous solution) and heated at 95°C for 5 minutes. After that, sonication was performed in ice water for 20 minutes. 3000 g was centrifuged at 4°C for 5 minutes, and the supernatant was collected to separate the coatings that had shrunk due to heating. 0.2 mL of the supernatant was transferred to another tube, and 8.7 μL of TCEP and 8.7 μL of iodoacetamide were added for reductive alkylation treatment. Methanol, chloroform, and ultrapure water were added in that order, stirred and mixed, and then centrifuged. Each sample was placed in an S-Trap. TM The samples were loaded onto micro columns (ProtiFi). After loading, the samples were washed with 90% methanol / 10% 1 mol / L TEAB aqueous solution and 50% chloroform / 50% methanol. Next, trypsin and Lys-C were added, and the samples were enzymatically digested by incubation at 37°C overnight. 40 μL of 50 mmol / L TEAB aqueous solution, 40 μL of 0.2% formic acid aqueous solution, and 40 μL of 50% acetonitrile aqueous solution were added sequentially to elute the digested peptides. The eluates from each stage were pooled together and dried by vacuum centrifugation. After desalting using GL-Tip SDB (GL Sciences Co., Ltd.), the samples were dried by vacuum centrifugation. Finally, 30 μL of 2% acetonitrile aqueous solution containing 0.1% formic acid was added to dissolve the peptides, and the peptide concentration was measured.

[0087] <Liquid Chromatography Conditions> The LC-UV analysis was performed under the following conditions. Mobile phase: A) 0.1% formic acid aqueous solution, B) 80% acetonitrile aqueous solution containing 0.1% formic acid Desalination column: Acclaim, manufactured by Thermo Fisher Scientific. PepMap 100 Nano Trap C18 nano Viper (inner diameter 75μm, length 20mm, particle size 3μm) Separation column: Acclaim, manufactured by Thermo Fisher Scientific. PepMap 100 RSLC, C18 (inner diameter 75 μm, length 150 mm, particle size 2 μm) Elution rate: 0.3μL / min Solvent gradient conditions: B5%(0-5min)→B50%(20min)→B95%(21-45min)→B5%(46-60min) Column temperature: 40℃ Sample injection volume: 1 μL Detection wavelength: UV 214nm

[0088] (Example 2-1) In Example 1-1, instead of spraying electrostatic spray directly onto the skin, 100% rayon with a basis weight of 80 g / m² was applied to a metal collection plate. 2 A nonwoven fabric (manufactured by Ikeda Paper Industry Co., Ltd.) was placed as a base material, and a coating consisting of fiber deposits was formed on one surface. The electrostatic spraying conditions were changed as follows, and the basis weight of the resulting coating was 2.5 g / m². 2 The method was changed to the following. The resulting film was cut into a circle with a diameter of 2.5 cm. Instead of forming the film by electrostatic spraying the composition, this film was applied to the cheek of the subject. After applying the film, a cosmetic emulsion (Est Biomimesis Veil Effector, manufactured by Kao Corporation) was applied over the film. This cosmetic emulsion contained water, a polyol, and an oil that is liquid at 20°C. The holding time was 2 hours. Except for these points, the deposit, which was the film, was collected along with the skin surface material in a non-invasive manner, similar to Example 1-1. <Conditions for electrostatic spraying> Voltage: 25kV Flow rate: 60mL / min Distance from nozzle to base nonwoven fabric: 16 cm Room temperature: 23℃, humidity: 35%RH In Experimental Example 2-1, 5.4 μg of protein was obtained from one membrane. Therefore, it was possible to perform the 0.5 μg peptide injection required for LC-MS under the above conditions.

[0089] (Example 2-2) In a test similar to that in Example 2-1, the basis weight of the coating was 0.4 g / m². 2 The method was changed to non-invasively collect the deposit, which is a film on the skin, along with the surface material.

[0090] (Examples 2-3) In a test similar to that in Example 2-1, the retention time for the film-forming state was changed to 1 hour. The film-forming deposit, along with the skin surface material, was collected non-invasively from the skin.

[0091] (Examples 2-4) In a test similar to that in Example 2-1, instead of forming a film by electrostatic spraying of the composition, a meltblown nonwoven fabric was attached to the forehead of the subject. The basis weight of the meltblown nonwoven fabric was 3 g / m². 2 The average fiber diameter of the constituent fibers was 1 μm. Meltblown nonwoven fabric was used, cut into circles with a diameter of 2.5 cm. The retention time for the coating was set to 5 hours. The coating, which was the deposited material, was collected non-invasively from the skin along with the skin surface material.

[0092] (Examples 2-5) In a test similar to that in Example 2-1, the retention time of the coating was set to 6.5 hours. The coating, which was a deposit, was collected along with the skin surface material in a non-invasive manner.

[0093] (Comparative Examples 2-1, 2-2) The same test as in Experimental Example 1 (2) using oil-absorbing film was conducted. In other words, in the same manner as in Experimental Example 1 (2), after washing the face, the skin of the subject's cheek was thoroughly rubbed with one oil-absorbing film on the left cheek to collect surface skin material. The obtained oil-absorbing film was collected in the same manner as in Experimental Example 1 (2), and protein extraction and analysis were performed. However, in this example, the time between washing the face and wiping with an oil-absorbing film was set to 2 hours and 6.5 hours. In Comparative Example 2-1, only 0.54 μg of protein was obtained from the film used in a single sample, and in Comparative Example 2-2, only 0.39 μg was obtained. Therefore, it was difficult to inject the same amount of peptide as the film coating using LC-MS under the above conditions. The adhesiveness, peelability, and appearance of the deposits in Examples 2-1 to 2-5 and Comparative Examples 2-1 and 2-2 were evaluated using the method described above. Furthermore, the amount of recovered protein was determined for Examples 2-1 to 2-5 and Comparative Examples 2-1 and 2-2 using the method described above. The results are shown in Table 3. From Table 3, it can be seen that the amount of recovered protein was high in the examples, indicating that more protein can be obtained by the present invention than when using an oil-absorbing film.

[0094] [Table 3]

[0095] (Experimental Example 3) (Comparative Example 3-1) A spunbond nonwoven fabric made of PP was used. The basis weight of the spunbond nonwoven fabric was 17 g / m². 2 The average fiber diameter of the constituent fibers was 15 μm. The spunbond nonwoven fabric was cut to 3 cm x 3 cm. In a test similar to that of Example 1-1, instead of forming a film by electrostatic spraying the composition, this spunbond nonwoven fabric was attached to the right cheek of the subject, and the same test was performed. The nonwoven fabric fell off the skin after about 5 minutes, indicating that it could not be attached for a long period of time.

[0096] (Example 3-1) In a test similar to that in Example 2-1, the basis weight of the coating was 50 g / m². 2 The method was changed to the following. The capsule was also applied to the forehead of the subject. The peptide injection amount was 0.055 μg. The capsule, which is a deposit, was collected along with the skin surface material in a non-invasive manner. Table 4 shows that protein can be collected from the forehead, that the present invention has excellent long-term adhesion properties by using a deposit of specific fibers, and that it also has excellent appearance when applied.

[0097] [Table 4] [Industrial applicability]

[0098] The present invention provides a method for efficiently collecting skin surface substances, which are endogenous substances present on the stratum corneum of the skin, using a method that allows for long-term application. By analyzing the skin surface substances obtained using this invention, it becomes possible to monitor the health status of the individual. [Explanation of symbols]

[0099] 10 Electrostatic spray device 11 Low-voltage power supply 12 High-voltage power supply 13. Auxiliary Electrical Circuits 15A Micro Gear Pump 14 Container 15 nozzles 15B Pipeline 15C Flexible conduit 19 Current-limiting resistor

Claims

1. The process involves applying a deposit containing fibers with an average fiber diameter of 0.01 μm to 7 μm to the skin and holding it for 0.5 hours or more to allow skin surface substances, which are internally derived substances present on the stratum corneum of the skin, to adhere to the deposit; The process includes a removal step for removing the deposits that have undergone the adhesion step from the skin, A method for collecting surface material, which involves collecting the deposit after a removal process to which surface material is attached.

2. The method for collecting a skin surface substance according to claim 1, wherein the aforementioned fibers include a thermoplastic resin.

3. The basis weight of the aforementioned sediment is 0.1 g / m². ２ 50g / m or more ２ The method for collecting skin surface material according to claim 1 or 2, which is as follows:

4. A method for collecting skin surface material according to claim 1 or 2, wherein a liquid agent containing one or more selected from water, a polyol, and an oil agent that is liquid at 20°C is applied to the skin before or during the adhesion process.

5. The method for collecting a skin surface substance according to claim 1 or 2, wherein the skin surface substance contains a hydrophilic component and a hydrophobic component.

6. A method for examining a skin surface material obtained by the sampling method described in claim 1 or 2, A method for inspecting surface material, comprising the step of separating surface material from the recovered sediment.

7. The method for inspecting skin surface material according to claim 6, wherein the skin surface material is separated from the deposit by immersing the deposit in an inspection solution and extracting the skin surface material.

8. A method for inspecting a skin surface according to claim 6, comprising inspecting the amount and / or type of compounds contained in the skin surface.

9. A method for examining a skin surface material obtained by the sampling method described in claim 2, The method for inspecting a surface material according to claim 6, further comprising the step of heating the deposit at a temperature between the glass transition temperature of the thermoplastic resin and 160°C.

10. A method for collecting skin surface material according to claim 1 or 2, wherein the attachment step involves performing a step selected from the following (I-1) and (I-2). (I-1) A step of electrostatically spraying onto the skin a composition containing one or more volatile substances selected from water, alcohol, and ketones, and a polymer having fiber-forming ability. (I-2) A step of applying a deposit of fibers with an average fiber diameter of 0.01 μm or more and 7 μm or less, obtained by electrospinning or melt blowing, to the skin.

11. A fibrous deposit used to collect epidermal substances, which are endogenous substances present on the stratum corneum of the skin, Sediments containing fibers with an average fiber diameter of 0.01 μm to 7 μm.

12. A test kit comprising the sediment described in claim 11 and a liquid agent, The aforementioned test kit is The aforementioned deposit is applied to the skin, By holding it for 0.5 hours or more, the skin surface substances, which are internally derived substances present on the stratum corneum of the skin, adhere to the deposits. Next, the deposit is removed from the skin, and the removed deposit is collected. The liquid agent is applied to the skin before or while the aforementioned skin surface material is attached to the aforementioned deposit, The aforementioned liquid preparation contains one or more selected from water, polyol, and oils that are liquid at 20°C. Test kit.

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