Dry composition containing flap end nuclease
JP2026110664APending Publication Date: 2026-07-02GEN PROBE INC
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- GEN PROBE INC
- Filing Date
- 2026-04-17
- Publication Date
- 2026-07-02
AI Technical Summary
Technical Problem
The stability of freeze-dried materials used in nucleic acid-based assays is affected by the hygroscopicity of salts, leading to unwanted rehydration and reduced activity, necessitating refrigerated storage to maintain effectiveness.
Method used
A composition comprising flap endonuclease, extender, and organic buffer with an inorganic salt concentration of 6 mM or less, and being glycerol-free, along with specific concentrations of oligonucleotides and polymerases, is developed to enhance stability and activity.
Benefits of technology
The composition maintains robust assay performance even after prolonged exposure to humid environments, ensuring stable activity and effective nucleic acid-based assays without refrigeration.
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Abstract
A dry composition containing flap end nuclease is provided. [Solution] A composition of an aqueous solution comprising or essentially comprising a flap endonuclease, an extender, and an organic buffer is disclosed, the aqueous solution having an inorganic salt concentration of 5 mM or less, and the composition is substantially glycerol-free. The present invention aims to provide an improvement to lyophilized compositions, particularly when used in nucleic acid-based assays, for example, in non-amplification assays or non-amplification assays combined with amplification assays.
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