UV absorber remover

Abstract

We provide naturally derived materials that are less irritating to the skin and can efficiently remove UV absorbers from the skin. [Solution] A UV absorber remover containing lactic acid bacteria culture as an active ingredient.

Description

Technical Field

[0001] The present invention relates to an ultraviolet absorber remover that efficiently removes an ultraviolet absorber from the skin.

Background Art

[0002] Sunblock cosmetics containing an ultraviolet absorber, especially those with high SPF and PA values, adhere closely to the skin, so they may not be completely removed by ordinary face washing or showering. In addition, sunblock cosmetics often have a water-resistant function. In particular, since the ultraviolet absorber itself is an oily component, ingenuity is required when removing it from the skin after its function is completed.

[0003] To remove an ultraviolet absorber, a cleansing agent is mainly used. However, a surfactant-based cleansing agent may strongly stimulate the skin because it emulsifies and washes away oily components. In addition, oil cleansing effectively dissolves makeup and sebum, but oil may remain after use, and double washing may be required in some cases. Micellar water removes dirt using minute spheres (micelles) containing both water and oil, but the cleansing effect on oily components may be low because of the small amount of oily components.

[0004] The supernatant of a lactic acid bacterium culture is known to have no skin irritation and to have a moisturizing effect on the skin (Non-Patent Document 1). In addition, it has been reported that the culture supernatant of a lactic acid bacterium belonging to Streptococcus thermophilus has an anti-wrinkle formation effect (Patent Document 1).

[0005] However, it is not known that a lactic acid bacterium culture is effective in removing an ultraviolet absorber from the skin.

Prior Art Documents

Patent Documents

[0006]

Patent Document 1

Non-Patent Documents

[0007] [Non-Patent Document 1] Journal of the Japanese Society of Cosmetic Science, 6(4), p238, 1982 [Overview of the Initiative] [Problems that the invention aims to solve]

[0008] This invention relates to providing a naturally derived material that is less irritating to the skin and can efficiently remove UV absorbers from the skin. [Means for solving the problem]

[0009] The inventors of this invention conducted intensive research to solve the above problems and discovered that a culture of lactic acid bacteria has the effect of efficiently removing ultraviolet absorbers from the skin.

[0010] In other words, the present invention relates to the following 1) to 6). 1) A UV absorber remover containing lactic acid bacteria culture as an active ingredient. 2) The ultraviolet absorber remover described in 1), wherein the lactic acid bacteria culture is a culture obtained by culturing lactic acid bacteria in a culture medium containing milk. 3) The ultraviolet absorber remover described in 1), wherein the lactic acid bacteria culture is a low molecular weight fraction of 20,000 Da or less obtained by fractionating the supernatant of a culture obtained by culturing lactic acid bacteria in a milk-containing medium. 4) A UV absorber remover as described in any of 1) to 3), wherein the lactic acid bacteria are lactic acid bacteria belonging to Streptococcus thermophilus. 5) A UV absorber remover according to any of 1) to 3), wherein the lactic acid bacteria is Streptococcus thermophilus YIT 2084 strain (FERM BP-10879). 6) A cleansing cosmetic containing a UV absorber remover as described in any of 1) to 3). [Effects of the Invention]

[0011] The UV absorber remover of the present invention can efficiently remove UV absorbers from the skin without irritating it. Therefore, the UV absorber remover of the present invention is suitably used as an ingredient in cleansing cosmetics for removing sunscreen cosmetics from the skin. [Brief explanation of the drawing]

[0012] [Figure 1] A diagram showing the removal rate of ethylhexyl methoxycinnamate by lactic acid bacteria culture. [Modes for carrying out the invention]

[0013] In the present invention, the lactic acid bacteria culture can be a culture obtained by culturing in a medium containing materials that can be fermented by lactic acid bacteria, such as milk (for example, cow's milk, human milk, goat's milk, cream, skim milk powder, etc.) or soy milk, and may or may not contain lactic acid bacteria cells. That is, the lactic acid bacteria culture of the present invention includes the supernatant obtained by removing insoluble substances such as bacterial cells after culturing lactic acid bacteria by filtration, centrifugation, etc., and it is preferable to use the culture supernatant from the viewpoint of removing ultraviolet absorbers.

[0014] The materials that can be fermented by lactic acid bacteria used in the culture medium are preferably animal milks such as cow's milk, human milk, goat's milk, cream, and skim milk powder. These may be processed by filtration, centrifugation, dissolution or dilution with a solvent, grinding with a mixer, enzymatic treatment with amylase, cellulase, pectinase, protease, etc., or extraction with a solvent. Such materials may be used as is or diluted to an appropriate concentration as a culture medium, or they may be added to any culture medium such as MRS medium or GAM medium.

[0015] Furthermore, the culture medium may be appropriately formulated with nutrients such as yeast extract, chlorella extract, vitamins (vitamin A, vitamin B, vitamin C, vitamin E, etc.), protein hydrolysates including various peptides, amino acids, minerals, salts, surfactants (polysorbate 80, etc.), fatty acids (oleic acid, etc.), and metals (calcium, magnesium, manganese, etc.).

[0016] A more suitable culture medium from the standpoint of removing UV absorbers is a skim milk culture medium obtained by dissolving skim milk in water and heat-sterilizing it.

[0017] For example, the culture can be carried out by adding lactic acid bacteria to a culture medium at a concentration of 0.01 to 10% by mass, preferably 0.1 to 5% by mass, and culturing it at 20 to 45°C, preferably 25 to 42°C, more preferably 37 to 42°C for 1 to 96 hours, preferably 3 to 96 hours, more preferably 4 to 30 hours. Other culture conditions such as standing, stirring, shaking, and aeration can also be appropriately selected to suit the growth of lactic acid bacteria, but stirring culture is preferred.

[0018] In the present invention, the lactic acid bacteria are not particularly limited. For example, Lactobacillus paracasei (former name: Lactobacillus paracasei), Lactobacillus casei (former name: Lactobacillus casei), Lactobacillus zeae (former name: Lactobacillus zeae), Lactobacillus rhamnosus (former name: Lactobacillus rhamnosus), etc. of the genus Lactobacillus, Lactobacillus crispatus, Lactobacillus amylovorus, Lactobacillus buchneri, etc. of the genus Lactobacillus, Lactiplantibacillus plantarum (former name: Lactobacillus plantarum), Lactiplantibacillus pentosus (former name: Lactobacillus pentosus), etc. of the genus Lactiplantibacillus, Limosilactobacillus fermentum (former name: Lactobacillus fermentum), etc. of the genus Limosilactobacillus, Levilactobacillus brevis (former name: Lactobacillus brevis), etc. of the genus Levilactobacillus, Lentilactobacillus buchneri (former name: Lactobacillus buchneri), Lentilactobacillus parabuchneri (former name: Lactobacillus parabuchneri), etc. of the genus Lentilactobacillus, Ligilactobacillus maris (former name: Lactobacillus maris), etc. of the genus Ligilactobacillus, Lactococcus lactis, etc. of the genus Lactococcus, Leuconostoc mesenteroides, Leuconostoc carnosum, Leuconostoc citreum, Leuconostoc gelidum, Leuconostoc lactis, etc. of the genus Leuconostoc, Pediococcus pentosaceus, etc. of the genus Pediococcus, Enterococcus faecalis, Enterococcus faecium, etc. of the genus Enterococcus, Weissella confusa, Weissella paramesenteroides, Weissella viridescens, etc. of the genus Weissella, Streptococcus thermophilus, etc. of the genus Streptococcus, and other lactic acid bacteria can be mentioned. These lactic acid bacteria may be used alone or in combination of two or more.

[0019] In recent years, lactic acid bacteria of the genus Lactobacillus have been reclassified (Zheng et al., A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int. J. Syst. Evol. Microbiol. 2020 Apr; 70(4):2782-2858 DOI 10.1099 / ijsem.0.004107).

[0020]

Table 1

[0021] Among these, lactic acid bacteria such as Streptococcus thermophilus of the genus Streptococcus, Lactobacillus paracasei and Lactobacillus casei of the genus Lactobacillus casei, and Lactiplantibacillus plantarum of the genus Lactiplantibacillus are preferred. Among these lactic acid bacteria, lactic acid bacteria of the genus Streptococcus are preferred, and Streptococcus thermophilus is more preferred. Furthermore, among them, there are Streptococcus thermophilus YIT strain 2084 (FERM BP-10879, deposit date: August 18, 2006), Streptococcus thermophilus YIT strain 2085 (FERM BP-10880, deposit date: August 18, 2006), Streptococcus thermophilus YIT strain 2021 (FERM BP-7537, deposit date: November 1, 1996), Streptococcus thermophilus YIT strain 2059 (FERM BP-10878, deposit date: August 18, 2006), and Streptococcus thermophilus YIT strain 2001 (FERM BP-7538 (deposited on January 31, 2001) is preferred, and Streptococcus thermophilus YIT 2084 strain (FERM BP-10879, deposited on August 18, 2006) is particularly preferred.

[0022] The obtained lactic acid bacteria culture can be used as is after heat sterilization as needed (usually at 60-85°C for 2-60 minutes), but insoluble matter can also be removed from the culture by means of centrifugation, and the supernatant can be used. Furthermore, the supernatant from which bacterial cells and other materials have been removed by filtration, dialysis, etc., can also be used. In addition, a specific fraction may be separated by extraction treatment with a solvent, gel filtration, or other separation methods, and this can be used. Freeze-drying or concentration to dryness can also be performed.

[0023] A preferred embodiment of the lactic acid bacteria culture is the supernatant obtained by removing insoluble matter from a culture of lactic acid bacteria cultured in a milk-containing medium (referred to as "lactic acid bacteria culture (milk)") by filtration, centrifugation, etc. Furthermore, a low molecular weight fraction of 20,000 Da or less, which is fractionated from the supernatant by separation means such as ultrafiltration, gel filtration, or dialysis, can be preferred.

[0024] The lactic acid bacteria culture obtained in this manner exhibits an excellent removal effect on ethylhexyl methoxycinnamate, a typical ultraviolet absorber, as shown in the examples described later. Therefore, the lactic acid bacteria culture of the present invention can serve as a UV absorber remover for removing UV absorbers from the skin, and can be used to remove UV absorbers from the skin.

[0025] Here, the UV absorber is not particularly limited, and examples include those commonly used in cosmetics. Examples of UV absorbers include ethylhexyl methoxycinnamate (also known as octinoxate), octocrylene, t-butyl methoxydibenzoylmethane (also known as avobenzone), ethylhexyl triazone, diethylamino hydroxybenzoyl hexyl benzoate, oxybenzone-3, methylene bisbenzotriazolyltetramethylbutylphenol (also known as bisoctrizole), phenylbenzimidazole sulfonic acid (also known as ensulizol), homosalate, ethylhexyl salicylate (also known as octisalate), bisethylhexyloxyphenol methoxyphenyl triazine (also known as bemotoridinol), etc. Of these, the UV absorber remover of the present invention is suitable for removing ethylhexyl methoxycinnamate.

[0026] In the present invention, removal of UV absorbers means removing UV absorbers that have adhered to the skin from the skin. The effect of the UV absorber remover of the present invention on removing UV absorbers from the skin can be evaluated in accordance with the method for evaluating the cleansing power of cleansing cosmetics using a filter material described in Japanese Patent No. 7496305. In other words, the UV absorber removal effect of the UV absorber remover can be evaluated by (1) adding a UV absorber to the filter media, (2) adding a UV absorber remover to the filter media obtained in step (1) to bring the UV absorber and the UV absorber remover into contact, and (3) adding a solvent for removing the UV absorber to the filter media obtained in step (2), performing filtration, and measuring the amount of UV absorber remaining in the filter media or measuring the amount of UV absorber contained in the filtrate.

[0027] The lactic acid bacteria culture according to the present invention can be used as is, or in combination with other formulation materials, such as water, alcohols, oil components, surfactants, preservatives, fragrances, pigments, humectants, thickeners, antioxidants, chelating agents, pH adjusters, foaming agents, powders, etc., to form a composition for removing ultraviolet absorbers. However, a preferred form of use is in incorporating it into cleansing compositions prepared in various product forms, such as cleansing cosmetics.

[0028] Examples of cleansing compositions containing the lactic acid bacteria culture according to the present invention include oily cleansing agents such as cleansing oils, emulsifying cleansing agents such as cleansing creams, bicontinuous structure type cleansing agents in which aqueous and oily components form continuous phases, liquid crystal structure type cleansing agents that form higher-order aggregates of amphiphilic molecules such as cleansing gels, and aqueous cleansing agents such as cleansing lotions.

[0029] The amount of lactic acid bacteria culture to be incorporated into a cleansing composition is not particularly limited and can be set appropriately depending on the desired product form, but it is preferably 0.00001 to 4% by mass, more preferably 0.01 to 3% by mass, and even more preferably 0.1 to 2% by mass in terms of the mass (dry solids) of the lactic acid bacteria culture in the composition. [Examples]

[0030] The present invention will be specifically described below with reference to examples, but these examples are not intended to limit the scope of the present invention.

[0031] Preparation Example 1: Preparation of Lactic Acid Bacteria Culture A strain of Streptococcus thermophilus YIT 2084 (FERM BP-10879), preserved at -80°C, was inoculated into a 2 mL test tube of M-17 medium (Difco) containing 10 g / L lactose using one loop of platinum, and incubated overnight at 40°C. Next, this culture was inoculated into 2 mL of 10% by mass skim milk powder aqueous solution (Difco) to a concentration of 1% by mass, and incubated overnight at 40°C to prepare the pre-culture. The pre-culture was then inoculated into 100 mL of the main culture medium (3% by mass skim milk powder aqueous solution) at a concentration of 1% by mass, and incubated at 40°C for 24 hours. The cultured bacterial suspension was centrifuged at 4°C at 8,000 × g for 15 minutes, and the precipitate was removed. The obtained precipitate removal solution (supernatant) was subjected to ultrafiltration at 4°C and 3,000 × g for 1 hour using a centrifugal ultrafiltration filter with a fractional molecular weight cutoff of 20,000 Da (product name "Centricut Mini V-20," manufactured by Kurabo Industries Ltd.) to obtain 10 mL of the low molecular weight fraction of the lactic acid bacteria culture (milk). This low molecular weight fraction contained 2% (20,000 μg / mL) of the dry solids of the lactic acid bacteria culture. The average molecular weight of this low molecular weight fraction of the lactic acid bacteria culture (milk) was approximately 300 Da.

[0032] The average molecular weight was measured by dissolving the low molecular weight fraction in a 50 mM sodium chloride solution and then performing HPLC under the following conditions.

[0033] (HPLC conditions) Equipment: Waters-600E Detector: ISIRI-980 Column: ShodexSUGARKS-804 Column temperature: 80℃ Mobile phase: 50mM NaCl Flow rate: 1mL / min Injection volume: 10μL

[0034] Test Example 1: Removal effect of UV absorbers

[0035] 1. Method In accordance with the method for evaluating the cleansing power of a cleansing cosmetic using a filter material described in Patent No. 7496305, the removal rate of the lactic acid bacteria culture from the ultraviolet absorber (ethylhexyl methoxycinnamate) was measured as follows. An ethanol solution of ethylhexyl methoxycinnamate (ethylhexyl methoxycinnamate concentration 1 v / v%) was added to the center of a filter paper (qualitative filter paper No. 1, retaining particle size 6 μm, diameter 55 mm, thickness 0.2 mm, water absorption 9.0 cm, manufactured by ADVANTEC) using a pipette, and left to stand for 24 hours. Subsequently, the aforementioned filter paper was placed in the filtration apparatus, and 100 mL of the sample (a solution prepared by adjusting the pH of the low molecular weight fraction of the lactic acid bacteria culture (milk) obtained in Preparation Example 1 to pH 5.8 with NaOH water, and then diluting it with physiological saline (lactic acid bacteria culture solution; concentration 10-90% by mass) was poured in, and natural filtration was performed. The resulting filter paper was immersed in 10 mL of ethanol to extract the ethylhexyl methoxycinnamate remaining on the filter paper, and the absorbance (310 nm) of the extract was measured using a spectrophotometer (U-5100, manufactured by Hitachi High-Tech Science Corporation) (A: absorbance after filtration). As a control, 10 μL of an ethanol solution of ethylhexyl methoxycinnamate (ethylhexyl methoxycinnamate concentration 1 v / v%) was added by pipette, and the filter paper, which had been allowed to stand for 24 hours, was extracted without filtration, and the absorbance (310 nm) was measured (B: absorbance before filtration).

[0036] As a correction for absorbance derived from the sample, untreated filter paper with ethylhexyl methoxycinnamate was placed in the filtration apparatus, and the same filtration and extraction processes were performed, and the absorbance (310 nm) was measured (C: absorbance derived from the sample). The removal rate of ethylhexyl methoxycinnamate was determined using the following formula. Each operation was performed three times. The average removal rate of ethylhexyl methoxycinnamate is shown in Figure 1.

[0037]

number

[0038] 2.Results From Figure 1, The low-molecular-weight fraction of lactic acid bacteria culture showed excellent removal efficacy against ethylhexyl methoxycinnamate. Furthermore, it was confirmed that using the low-molecular-weight fraction of lactic acid bacteria culture at a high concentration (70% or more) resulted in a high removal rate.

[0039] Prescription examples Prepare a cleansing cosmetic containing the lactic acid bacteria culture shown below.

[0040] [Table 2] 1. While raising the temperature to 80°C, add ingredients No. 1 to 12 sequentially and dissolve them. 2. After cooling to below 40°C, add No. 13 and 14, and adjust the pH with No. 15 and 16 (pH 5.0-6.0).

[0041] [Table 3] 1. While raising the temperature to 80°C, add ingredients No. 1 to 8 sequentially and dissolve them. 2. Dissolve No. 9 in No. 10 and add it to step 1 at 80°C. 3. After cooling to below 40°C, add No. 11.

[0042] [Table 4] 1. Heat and dissolve A and B. 2. Add A to B and emulsify using a homomixer. 3. Cool to room temperature while stirring.

[0043] [Table 5] 1. Heat and dissolve A and B. 2. Add B to A and emulsify using a homomixer. 3. Cool to room temperature while stirring.

[0044] [Table 6] 1. Heat and dissolve A and B. 2. Add B to A and stir until a uniform gel is formed. 3. Cool to room temperature while stirring.

[0045] [Table 7] 1. Heat A and B until dissolved, then mix them together. 2. Add C to the mixture from step 1 and stir. 3. Cool to room temperature while stirring.

[0046] [Table 8] 1. Heat A to dissolve it, then add B to form a gel. 2. Cool to room temperature while stirring.

[0047] [Table 9] 1. Mix Nos. 1-7 at room temperature, then add No. 10 and dissolve. 2. Adjust the pH using No. 8-10 (pH 5.0-6.0).

Claims

1. A UV absorber remover containing lactic acid bacteria culture as its active ingredient.

2. The ultraviolet absorber remover according to claim 1, wherein the lactic acid bacteria culture is a culture obtained by culturing lactic acid bacteria in a culture medium containing milk.

3. The ultraviolet absorber remover according to claim 1, wherein the lactic acid bacteria culture is a low molecular weight fraction with a molecular weight of 20,000 Da or less, fractionated from the supernatant of a culture obtained by culturing lactic acid bacteria in a milk-containing medium.

4. The ultraviolet absorber remover according to any one of claims 1 to 3, wherein the lactic acid bacteria are lactic acid bacteria belonging to Streptococcus thermophilus.

5. The ultraviolet absorber remover according to any one of claims 1 to 3, wherein the lactic acid bacterium is Streptococcus thermophilus YIT 2084 strain (FERM BP-10879).

6. A cleansing cosmetic containing the UV absorber remover described in any one of claims 1 to 3.

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