Bacteria with high carbon dioxide yield relative to carbon and gene knockout strains of those bacteria that disrupt the metabolic enzymes of those bacteria.

Metabolic enzyme gene knockout strains in actinomycetes, particularly Streptomyces, address the challenge of low carbon dioxide-to-carbon yields by disrupting relevant enzymes, achieving enhanced yields and maintaining growth, suitable for metabolite production.

JP2026113615APending Publication Date: 2026-07-07NAGASE & CO LTD
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
NAGASE & CO LTD
Filing Date
2026-04-02
Publication Date
2026-07-07

AI Technical Summary

Technical Problem

Existing bacterial strains face challenges in achieving high carbon dioxide-to-carbon yields due to the disruption of metabolic enzymes, leading to decreased growth ability and sugar consumption, particularly in actinomycetes like Corynebacterium and Escherichia coli.

Method used

Development of metabolic enzyme gene knockout strains, specifically in actinomycetes, where enzymes responsible for carbon dioxide production in pathways such as glycolysis, pentose phosphate, and citric acid are disrupted, maintaining or enhancing growth ability while increasing carbon dioxide yield to exceed 50% during aerobic culture.

Benefits of technology

The metabolic enzyme gene knockout strains exhibit improved carbon dioxide-to-carbon yields, outperforming control strains by up to 1.5 times, facilitating efficient metabolite production and maintaining growth ability.

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Abstract

This invention provides a bacterium with a high carbon-to-carbon yield of carbon dioxide and a gene knockout strain of the bacterium's metabolic enzymes. It also provides a method for isolating the bacterium with a high carbon-to-carbon yield of carbon dioxide. Furthermore, it provides a method for producing, culturing, and using the gene knockout strain of the bacterium's metabolic enzymes. [Solution] The present invention provides a metabolic enzyme gene knockout strain of actinomycetes in which the carbon dioxide (CO2) yield relative to carbon exceeds 50% during at least the logarithmic growth phase or stationary phase in aerobic culture in a medium containing a sufficient amount of carbon source and other nutrients, wherein at least one of the genes encoding enzymes responsible for the metabolic reaction that produces CO2 is disrupted, and the CO2 yield relative to carbon is reduced compared to a control actinomycete of the same species in which none of the genes are disrupted.
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