Spray-dried product containing galactomannan hydrolysates

Abstract

The present invention relates to providing a novel spray-dried product with excellent solubility and a method for producing the same. [Solution] A spray-dried product containing galactomannan hydrolysate, with a bulk density of 0.15 to 0.40 g / ml and an average particle size of 150 to 400 μm.

Description

Technical Field

[0001] The present invention relates to a spray-dried product containing a galactomannan degradation product and a method for producing the same.

Background Art

[0002] Water-soluble dietary fibers are known to have effects such as improving the intestinal environment and suppressing the rise in postprandial blood glucose levels, and various preparations and food and drink products containing water-soluble dietary fibers have been proposed. For example, Patent Document 1 discloses a technique related to an intestinal environment-improving agent containing a galactomannan degradation product as an active ingredient. Supplements containing water-soluble dietary fibers include stick types of several grams and bag types of several tens of grams to several kilograms, and an intake method of adding them to food and drink products is recommended.

[0003] When a preparation for ingesting water-soluble dietary fiber is used by adding it to food and drink products, granulated products may be used to enhance solubility. For example, a solution containing water-soluble dietary fiber was spray-dried, and the obtained hollow particles were granulated and used. Also, in Patent Document 2, as a granulated product with excellent solubility and little dusting, it has been proposed to dry a solution containing water-soluble dietary fiber by a drying method other than spray drying to prepare solid particles and granulate them.

Prior Art Documents

Patent Documents

[0004]

Patent Document 1

Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0005] However, the preparation of granules requires numerous steps and considerable manufacturing time, resulting in low manufacturing efficiency. Therefore, there is a need for a more efficient formulation for consuming water-soluble dietary fiber.

[0006] The present invention relates to providing a novel spray-dried product with excellent solubility and a method for producing the same. [Means for solving the problem]

[0007] The present invention relates to the following [1] to [5]. [1] A spray-dried product containing galactomannan hydrolysate, with a bulk density of 0.15 to 0.40 g / ml and an average particle size of 150 to 400 μm. [2] A granulated product of a spray-dried material, wherein the spray-dried material contains a galactomannan hydrolysate, the bulk density of the spray-dried material is 0.15 to 0.40 g / ml, and the average particle size of the spray-dried material is 150 to 400 μm. [3] A food and beverage composition comprising the spray-dried material described in [1] and / or the granules described in [2]. [4] Food and beverages comprising the spray-dried material described in [1] and / or the granules described in [2]. [5] A method for producing a spray-dried product comprising the step of spray-drying a raw material solution mixed with a gas, wherein the raw material solution contains a galactomannan hydrolysate, the bulk density of the spray-dried product is 0.15 to 0.40 g / ml, and the average particle size is 150 to 400 μm. [Effects of the Invention]

[0008] According to the present invention, a novel spray-dried product with excellent solubility and a method for producing the same can be provided. [Brief explanation of the drawing]

[0009] [Figure 1] This is an electron microscope image (magnification: 500x) of the particles from Example 1. [Figure 2] This is an electron microscope image (magnification: 500x) of the particles in Comparative Example 1. [Modes for carrying out the invention]

[0010] When the inventors investigated the above problem, they newly discovered that by spray-drying a raw material solution mixed with gas, a spray-dried product (the spray-dried product of the present invention) with a bulk density and average particle size equivalent to that of conventional granules can be obtained. Furthermore, surprisingly, it was found that the spray-dried product of the present invention has higher solubility than granules with equivalent bulk density and average particle size. The mechanism of this improved solubility is unknown, but it is presumed that because the spray-dried product of the present invention is spherical and has holes in the particles (Figure 1), it does not form a film when it comes into contact with water, resulting in high water permeability and improved solubility.

[0011] The spray-dried product of the present invention contains galactomannan decomposition products. In this specification, "spray-dried product" refers to powder obtained using a spray drying apparatus.

[0012] Galactomannan hydrolysates are galactomannan with reduced molecular weight, and one or more types can be included in the spray-dried product. Galactomannan is a group of polysaccharides in which galactose (α-D-galactopyranose) is α-(1-6)-linked to a linear main chain (β-(1-4)-D-mannopyranose) made of mannose. Examples of galactomannan include guar gum, locust bean gum, tara gum, cassia gum, fenugreek gum, and sesbania gum, and examples of galactomannan hydrolysates include guar gum hydrolysate, locust bean gum hydrolysate, tara gum hydrolysate, cassia gum hydrolysate, fenugreek gum hydrolysate, and sesbania gum hydrolysate. Of these, it is preferable to include guar gum hydrolysate from the viewpoint of physiological regulatory function.

[0013] Galactomannan degradation products can be produced, for example, by hydrolysis in water using acid or high temperature and pressure, or by using enzymes, followed by obtaining fractions with a specific molecular weight distribution using chromatographic methods, or by decomposing fractions with a molecular weight above a certain level by oxidative decomposition using ozone. Alternatively, they can be produced by precisely controlling the reaction conditions in an enzymatic reaction, or by selecting galactomannan as the substrate, where the galactose side chains are coarse relative to the mannose backbone which is easily reacted by enzymes. In general, enzymatic production methods are preferred for the purpose of standardizing molecular weight.

[0014] The enzyme used in the enzymatic hydrolysis method is not particularly limited, as long as it is an enzyme that hydrolyzes linear mannose, and can be either commercially available or naturally derived. However, β-mannanase derived from Aspergillus or Rhizopus species is preferred.

[0015] While there are no particular limitations on the chromatographic separation method, a method using a porous gel as a support for separation based on molecular weight differences is promising. In this case, fractionation can be performed using a single-tube column chromatograph, but for industrial purposes, the use of a pseudo-moving-bed chromatograph that allows for continuous production is preferable.

[0016] The average molecular weight (Mw) of the galactomannan hydrolysate is not particularly limited, but from the viewpoint of solubility, it is preferably 2,000 to 40,000. Preferably it is 30,000 or less, more preferably 20,000 or less, and even more preferably 10,000 or less. The average molecular weight of the galactomannan hydrolysate in this specification is measured by the method described in the examples below. Examples of commercially available galactomannan hydrolysates with an average molecular weight of 2,000 to 40,000 include Sunfiber from Taiyo Lab Co., Ltd.

[0017] From the viewpoint of solubility, the content of galactomannan decomposition products in the spray-dried product of the present invention is preferably 50% by mass or more, more preferably 60% by mass or more, and even more preferably 80% by mass or more. The upper limit can be, for example, 100% by mass or less, 80% by mass or less, or 60% by mass or less.

[0018] The spray-dried product of the present invention can further contain, as optional components, water-soluble dietary fibers other than galactomannan degradation products, dextrins, saccharides, other polysaccharides, and the like. For example, it can further contain inulin, indigestible dextrin, polydextrose, gum arabic, psyllium seed gum, isomaltooligosaccharide, isomaltooligosaccharide, fructooligosaccharide, tamarind seed gum, karaya gum, glucomannan, pectin, cellulose, soybean polysaccharide, carrageenan, agar, alginic acid and its salts, xanthan gum, gellan gum, succinoglycan of Agrobacterium, carboxymethyl cellulose and its salts, and the like. Further, galactomannan that has not been depolymerized may be contained. Further, emulsifiers such as glycerin fatty acid esters, sucrose fatty acid esters, and lecithin, antioxidants such as ascorbic acid, tocopherol, flavonoids, and polyphenols, flavors, colorants, sweeteners, and useful intestinal bacteria such as lactic acid bacteria, bifidobacteria, saccharifying bacteria, and butyric acid bacteria, glucosinolates that are derivatives of glucose and amino acids, catechins such as ECg and EGCg, carotenoids such as astaxanthin and lutein, amino acids such as L-theanine and GABA, vitamins, minerals, and the like can be further contained.

[0019] The total amount of these optional components in the spray-dried product can be, for example, 0% by mass, 5% by mass or more, 10% by mass or more, 15% by mass or more, and also 95% by mass or less, 90% by mass or less, 85% by mass or less, and the like.

[0020] The bulk specific gravity of the spray-dried product of the present invention is 0.15 to 0.40 g / ml. From the viewpoint of handling properties such as transportation, it is preferably 0.20 g / ml or more, more preferably 0.25 g / ml or more, still more preferably 0.30 g / ml or more. Also, from the viewpoint of solubility, it is preferably 0.35 g / ml or less, more preferably 0.30 g / ml or less, still more preferably 0.25 g / ml or less, and still more preferably 0.20 g / ml or less. The bulk specific gravity in this specification is measured by the method described in the examples below.

[0021] The average particle size of the spray-dried product of the present invention is 150 to 400 μm, and from the viewpoint of solubility, it is preferably 200 μm or more, more preferably 250 μm or more, and still more preferably 300 μm or more. The average particle size in this specification is measured by the method described in the examples below.

[0022] The dietary fiber content in the spray-dried product of the present invention is preferably 50% or more, and can be 60% or more, 70% or more, or 80% or more. The upper limit can be 100% or less, 95% or less, or 90% or less. The dietary fiber content in this specification is measured by the enzymatic gravimetric method described in AOAC 2009.01.

[0023] The spray-dried product of the present invention has excellent solubility and can be suitably used by incorporating it into various foods and beverages. Accordingly, the present invention also provides foods and beverages containing the spray-dried product of the present invention. Examples of foods and beverages include instant foods such as instant noodles, retort foods, canned goods, microwaveable foods, instant soups and miso soups, and freeze-dried foods; beverages such as soft drinks, fruit juices, vegetable drinks, soy milk drinks, coffee drinks, tea drinks, powdered drinks, concentrated drinks, nutritional drinks, and alcoholic beverages; wheat flour products such as bread, pasta, fresh noodles, cake mixes, fried chicken batter, and breadcrumbs; confectionery such as caramel, candy, chewing gum, chocolate, cookies, biscuits, cakes, pies, snacks, crackers, Japanese sweets, and dessert confectionery; sauces, tomato-based seasonings, and more. This includes seasonings such as flavor enhancers, cooking mixes, sauces, dressings, soup bases, curry and stew mixes, processed oils and fats such as butter, margarine, and mayonnaise, dairy products such as milk beverages, yogurts, lactic acid bacteria beverages, ice cream, and creams, frozen foods, processed seafood products such as fish ham and sausages, processed livestock products such as meat ham and sausages, processed agricultural products such as canned agricultural products, jams and marmalades, pickles, boiled beans, and cereals, nutritional foods such as powders, tablets, capsules, and drinks, high-nutrient foods, liquid foods, concentrated liquid foods, and medical foods. In addition, it can be used in feed for livestock such as cattle, pigs, and poultry, and in feed for pet animals. There are no particular limitations on how it can be added to various foods and beverages; it can be added to raw materials in advance, during the manufacturing process, during cooking, or at the time of consumption. In this case, only the spray-dried product of the present invention may be added as a food and beverage composition, but a food and beverage composition containing a mixture of water-soluble dietary fibers other than galactomannan hydrolysate, amino acids, vitamins, minerals, etc., in addition to the spray-dried product of the present invention may also be used. Furthermore, the spray-dried product of the present invention can be consumed as a powder without being added to various foods and beverages.

[0024] As a preferred embodiment of the present invention, a manufacturing method is provided which includes a step of spray-drying a raw material solution containing galactomannan hydrolysate and mixed with gas (hereinafter also referred to as "gas-mixed solution"). The present invention will be described below, but the present invention is not limited to the manufacturing method of this embodiment.

[0025] The gas-mixed solution according to this embodiment can be prepared by injecting gas into a raw material solution containing water, a mixture of water-soluble dietary fibers other than galactomannan hydrolysate, a mixture of amino acids, vitamins, and minerals, and galactomannan hydrolysate. The raw material solution may contain the above-mentioned optional components in addition to galactomannan hydrolysate. Note that the gas injection is not limited to the above, and the solution may also be prepared by adding galactomannan hydrolysate and the above-mentioned optional components to a solvent that has been pre-injected with gas.

[0026] The type of gas used is not particularly limited; examples include nitrogen gas, carbon dioxide gas, nitrous oxide gas, and air, with nitrogen gas being preferred. One or more of these gases may be included. An example of a gas injection device is an ADS (manufactured by Anhydro). The gas injection rate varies depending on the desired bulk density, but can be, for example, 1 to 50 ml / min. Increasing the gas injection rate can lower the bulk density of the spray-dried material, while decreasing the injection rate can control the bulk density of the spray-dried material to a higher level.

[0027] Spray drying can be carried out using known spray drying equipment. The bulk density and average particle size of the resulting spray-dried material can be adjusted to a desired range by grinding using a roll crusher, cutter mill, self-grinding mill, stamp mill, stone mill type, sculpting machine, ring mill, power mill, roller mill, jet mill, hammer mill, pin mill, rotary mill, vibrating mill, planetary mill, etc., and by sizing using a classifier, sieving machine, screen, etc.

[0028] The spray-dried product of the present invention can also be further granulated to prepare granules. From the viewpoint of manufacturing efficiency, it is preferable to use the spray-dried product of the present invention, but by forming granules, further improvements in solubility and the expression of functions through mixing with other powders can be expected. Granulation can be carried out using known granulation equipment such as a fluidized bed granulator (manufactured by Powrec, product name FD-MP-01). The granules of the present invention can be suitably used by being incorporated into various food and beverage products, similar to the spray-dried product of the present invention. Accordingly, the present invention also provides food and beverage compositions and food and beverage products containing the granules of the present invention. [Examples]

[0029] The present invention will be specifically described below with reference to examples and comparative examples, but the present invention is not limited to these.

[0030] <Measurement of average molecular weight> The molecular weight of the galactomannan hydrolysates in each example and comparative example was analyzed using high-performance liquid chromatography. An aqueous solution of the galactomannan hydrolysate (solid content concentration: 1.0% (W / V)) was prepared as a sample solution, and after filtration of the sample solution, it was analyzed by high-performance liquid chromatography under the following conditions. HPLC (Shimadzu) analysis was performed (HPLC conditions: Column: TSKgel SuperAW 4000 (Tosoh) 6mm*150mm + TSKgel SuperAW 2500 (Tosoh) 6mm*150mm, Detector: RI (differential refractometer), Eluent: Water (100%), Flow rate: 0.3 mL / min, Column temperature: 80°C). A calibration curve showing the relationship between retention time and molecular weight was pre-determined using molecular weight markers (pullulan (molecular weight: 5,900, 11,800, 22,800, 47,300, 112,000, 212,000, 404,000, 788,000) and mannose, maltotriose, malthepeptaose).

[0031] <Measurement of bulk density> The bulk density was determined by measuring the granules to the level mark in a graduated cylinder cut at the 100ml mark, measuring the weight at that point, and using g / ml as the measurement value.

[0032] <Measurement of average particle size> The average particle size was measured using a dry particle size distribution analyzer (laser diffraction type, LS13 320 (BECKMAN COULTER)). The measurement conditions were air as the dispersion medium and a measurement time of 60 seconds. The particle size was calculated using the following conditions: the real part of the refractive index of the dispersion medium was 1.05, the real part of the refractive index of the sample was 0, and the imaginary part was 1. The average particle size was then calculated automatically.

[0033] Preparation of spray-dried material Example 1 900 parts by mass of water were mixed with hydrochloric acid to adjust the pH to 4.5. Then, 0.2 parts by mass of β-mannanase and 100 parts by mass of guar gum endosperm (Cymopsis tetragonolobus) were added to the water and mixed. The enzymes were allowed to react at 55°C to 65°C for 24 hours to hydrolyze the galactomannan contained in the guar gum. Next, the reaction mixture was heated at 90°C for 30 minutes to inactivate the enzyme and stop the hydrolysis reaction. The reaction mixture containing the hydrolysates was then filtered and separated by suction filtration, and the clear solution obtained by removing insoluble matter was concentrated under reduced pressure (using a Yamato evaporator). A raw material solution with a solid content of 30% by mass was prepared. Subsequently, it was dried using a spray dryer (manufactured by Okawara Chemical Machinery Co., Ltd.) under nitrogen gas mixing to prepare a dried powder. The obtained dried powder was sieved through a sieve with a diameter of 20 cm and a mesh size of 350 μm, and 10 g of spray-dried guar gum hydrolysate was obtained as the sieved powder. Figure 1 shows an electron microscope image of the obtained particles (magnification: 500x).

[0034] Example 2 A spray-dried guar gum hydrolysate was obtained in the same manner as in Example 1, except that the dried powder was sieved through a sieve with a diameter of 20 cm and a mesh size of 300 μm.

[0035] Comparative Example 1 A spray-dried guar gum decomposition product was obtained in the same manner as in Example 1, except that nitrogen gas was not mixed in. An electron microscope image (magnification: 500x) of the obtained particles is shown in Figure 2.

[0036] Comparative Example 2 The spray-dried material of Comparative Example 1 was molded using water as a binder in a fluidized bed granulation and drying apparatus (manufactured by Powrec Co., Ltd.) to obtain granules of guar gum hydrolysate.

[0037] <Evaluation of solubility> The solubility of each example and comparative example was confirmed visually using the following method. The results are shown in Table 1. For substances that did not dissolve after more than 300 seconds, further measurements were not performed, and the result was simply labeled "over 300 seconds." Solubility of 300 seconds or less can be considered good. Measure 100 ml of deionized water into a 1200 ml glass beaker and heat it to 20°C in a constant temperature water bath. 2. Put the 5.0g of the measured sample into the weighing paper all at once. 3.3 seconds later, stir by hand with a spatula 30-40 times over 8-10 seconds. 4. Check the time it takes for the dissolution to complete.

[0038] [Table 1]

[0039] As shown in Table 1, it can be seen that the lower the bulk density, the better the solubility and the shorter the time it takes to dissolve. The spray-dried product obtained by the conventional method (Comparative Example 1) had a high bulk density, and its solubility was improved by granulation (Comparative Example 2). However, the spray-dried products of Examples 1 and 2 had low bulk density even without granulation, indicating superior solubility. Furthermore, although Example 1 and Comparative Example 2 had similar bulk densities, Example 1 showed superior solubility. This is thought to be because the particles have holes, as shown in Figure 1, which increases water permeability.

[0040] Furthermore, from the standpoint of handling during transportation, a high bulk density is desirable, but conventional methods have made it difficult to prepare granules with a bulk density of 0.25 g / ml or higher. On the other hand, in Example 2, a spray-dried product with a bulk density of 0.27 g / ml was obtained, resulting in a spray-dried product with excellent solubility and ease of handling.

[0041] Food and beverage preparation example 1 <Powdered soft drink> A powdered soft drink was prepared by mixing 30 g of the spray-dried product from Example 1 with 36 g of crystalline glucose, 25 g of powdered sugar, 6 g of anhydrous citric acid, and 3 g of trisodium citrate (crystalline). 20 g of the prepared powdered soft drink was added to 500 ml of water at 25°C, and no powdering was observed upon addition, and solubility was good.

[0042] Food and beverage preparation example 2 <white bread> 700g of strong flour, 12g of dry yeast, 1g of yeast food, and 420g of water were placed in a mixer bowl and mixed at low speed for 4 minutes and at medium speed for 2 minutes to obtain a starter dough. After fermenting the dough at 27°C for 4 hours, 300g of strong flour, 80g of sugar, 100g of fresh cream, 20g of salt, 3g of malt syrup, and 230g of water were added and mixed at low speed for 4 minutes and at high speed for 3 minutes. Then, 40g of butter and 20g of enzyme-containing shortening were added. 67g of the spray-dried material from Example 1 was added and mixed at low speed for 1 minute and at high speed for 3 minutes to obtain the final dough. After fermenting the dough at 27°C for 30 minutes, it was weighed out and divided into portions of 215g each, rounded, and then the dough was allowed to rest for another 20 minutes. Next, after shaping the dough, it was placed in a 1-loaf case and given a final fermentation for 100 minutes in a proofing tank at 38°C and 85% humidity. Then, the lid was placed over it and it was baked for 20 minutes in an oven with a top heat of 190°C and a bottom heat of 210°C to obtain a loaf of bread. When the spray-dried material from Example 1 was added during preparation, no powdery residue was observed, and it was uniformly mixed into the main dough. [Industrial applicability]

[0043] The spray-dried product of the present invention can be used in a variety of food and beverage products.

Claims

1. A spray-dried product containing galactomannan hydrolysate, with a bulk density of 0.15 to 0.40 g / ml and an average particle size of 150 to 400 μm.

2. The spray-dried product according to claim 1, wherein the galactomannan hydrolysate comprises one or more selected from the group consisting of guar gum hydrolysate, locust bean gum hydrolysate, tara gum hydrolysate, cassia gum hydrolysate, fenugreek gum hydrolysate, and sesbania gum hydrolysate.

3. The spray-dried product according to claim 1, wherein the average molecular weight of the galactomannan decomposition product is 2,000 to 40,000.

4. The spray-dried product according to claim 1, wherein the content of the galactomannan decomposition product is 50 to 100% by mass.

5. The spray-dried product according to claim 1, wherein the dietary fiber content is 50 to 100%.

6. The spray-dried product according to claim 1, wherein the bulk density is 0.25 to 0.40 g / ml.

7. The spray-dried product according to claim 1, wherein the spray-dried product is a spray-dried product obtained by spray-drying a raw material solution mixed with gas.

8. A granulated product of a spray-dried material, wherein the spray-dried material contains a galactomannan hydrolysate, the bulk density of the spray-dried material is 0.15 to 0.40 g / ml, and the average particle size of the spray-dried material is 150 to 400 μm.

9. A food and beverage composition comprising a spray-dried product according to any one of claims 1 to 7 and / or a granulated product according to claim 8.

10. Food and beverages comprising a spray-dried product according to any one of claims 1 to 7 and / or a granulated product according to claim 8.

11. A method for producing a spray-dried product, comprising the step of spray-drying a raw material solution mixed with a gas, wherein the raw material solution contains a galactomannan hydrolysate, the bulk specific gravity of the spray-dried product is 0.15 to 0.40 g / ml, and the average particle size is 150 to 400 μm.

12. The manufacturing method according to claim 11, wherein the gas comprises one or more selected from the group consisting of nitrogen gas, carbon dioxide gas, nitrous oxide gas, and air.

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