Method for detecting plant RNA viruses
The method of heat-treating a plant grinding solution with a primer at specific temperatures for one-step RT-PCR addresses labor and cost issues in conventional RT-PCR, improving detection efficiency and sensitivity.
JP2026115567AActive Publication Date: 2026-07-09NAT AGRI & FOOD RES ORG
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- NAT AGRI & FOOD RES ORG
- Filing Date
- 2024-12-27
- Publication Date
- 2026-07-09
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Abstract
This invention provides a plant RNA virus detection method that offers high detection efficiency while achieving labor savings and lower costs compared to conventional technologies. [Solution] A method for detecting plant RNA viruses, comprising the step of heat-treating a mixture obtained by mixing a plant pulverized liquid, which is obtained by grinding plants, with a primer solution, wherein the heat-treating step involves heat-treating the mixture at 60°C to 98°C for 4 to 6 minutes. Alternatively, the plant pulverized liquid is obtained by grinding plant tissue with a solution containing at least one selected from the group consisting of buffer and RNase-free water.
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