A virus precipitation phase forming agent for determining the antiviral activity of a test substance, and a method for determining the antiviral activity of a test substance using the same.

The use of PEG-based virus precipitation agents with real-time PCR allows for quick and precise identification of antiviral agents' ability to destroy virus shells, overcoming the limitations of conventional methods in assessing antiviral activity.

JP2026119481APending Publication Date: 2026-07-17OKUNO CHEM IND CO LTD
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
OKUNO CHEM IND CO LTD
Filing Date
2025-01-07
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing methods for determining antiviral activity of test substances are complicated and fail to accurately assess the ability of antiviral agents to destroy the outer shell of viruses, especially when additives like preservatives and excipients are present, making it difficult to identify effective antiviral agents.

Method used

A virus precipitation phase forming agent containing polyethylene glycol (PEG) with a molecular weight of 6000 to 9000, optionally with sodium chloride, is used to separate viral DNA/RNA by precipitating virus particles, followed by real-time PCR to determine antiviral activity.

Benefits of technology

Enables rapid and accurate determination of antiviral activity by distinguishing between agents that destroy the virus outer shell and those that inhibit cell contact, without requiring complex experiments, using standard biochemical equipment.

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Abstract

To provide a virus precipitation phase forming agent for determining the antiviral activity of a test substance, and a method for determining the antiviral activity of a test substance using the same, which can determine whether or not the test substance has antiviral activity against a given virus without performing complicated experiments or analyses. [Solution] The method for determining the antiviral activity of a test substance according to the present invention includes the steps of: preparing a mixed solution by adding the test substance to a test solution containing a virus; preparing a processed mixed solution containing a precipitated phase and a supernatant phase by adding a virus precipitating agent to the mixed solution; separating the precipitated phase from the processed mixed solution; and subjecting the precipitated phase to real-time PCR to determine whether or not there is amplification of genes contained in the precipitated phase. The virus precipitating agent according to the present invention contains polyethylene glycol.
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