Method for recovering nucleic acids derived from aquatic organisms

By contacting habitat water with an adsorbent and filtering it through specific pore size filters, followed by ion exchange column extraction, the method recovers nucleic acids from aquatic organisms non-invasively, achieving higher-resolution genetic analysis.

JP2026119775APending Publication Date: 2026-07-21KK TOYOTA CHUO KENKYUSHO
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Applications
Current Assignee / Owner
KK TOYOTA CHUO KENKYUSHO
Filing Date
2025-01-08
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing methods for recovering nucleic acids from aquatic organisms are inadequate for obtaining high-resolution genetic information, such as distinguishing between subspecies and varieties, and require invasive sampling that damages the target organisms.

Method used

A method involving contacting habitat water with an adsorbent that adsorbs microorganisms, followed by filtration using a specific pore size filter, and subsequent nucleic acid extraction using an ion exchange column method to recover longer DNA strands without damaging the organisms.

Benefits of technology

Enables non-invasive recovery of nucleic acids with higher mapping rates and longer DNA strands, allowing for more accurate genetic analysis, including subspecies identification and genetic diversity analysis.

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Abstract

Nucleic acids derived from aquatic organisms are non-invasively recovered to enable the acquisition of more comprehensive and accurate genomic DNA sequence information. [Solution] The method for recovering nucleic acids derived from aquatic organisms involves contacting the habitat water in which aquatic organisms live with an adsorbent that adsorbs microorganisms, and then filtering the habitat water that has come into contact with the adsorbent, thereby recovering a residue containing nucleic acids derived from aquatic organisms from the habitat water.
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