A hair amino acid penetration enhancer, and a method for enhancing the penetration of amino acids into hair.

Abstract

The present invention provides an agent for promoting the penetration of amino acids into hair, a method for promoting the penetration of amino acids into hair, and the like. [Solution] A hair penetration enhancer containing enzyme-treated royal jelly, and a method for promoting the penetration of amino acids into hair, comprising the step of applying the penetration enhancer to the hair.

Description

Technical Field

[0004] , ,

[0001] The present invention relates to a promoter for promoting the penetration of amino acids into hair, a method for promoting the penetration of amino acids into hair, and the like.

Background Art

[0002] Royal jelly is a milky substance synthesized in the salivary glands of honeybees and secreted from the hypopharyngeal gland and the major parotid gland located in the head. It is given by worker bees to the larval bees placed in the queen bee larvae's cells in order to create queen bees. Currently, royal jelly is widely used around the world in health foods, pharmaceuticals, cosmetics, etc. As its effectiveness, an increase in blood flow, a blood pressure lowering effect, a growth promoting effect, a sex hormone-like effect, an antibacterial effect, an antitumor effect, a wound healing promoting effect, an autonomic nerve disorder healing effect, an anti-stress effect, a calcium absorption promoting effect, a blood glucose level lowering effect, an antioxidant effect, etc. have been reported.

[0003] As causes of hair frizz and loss of hair strength, leakage of amino acids and proteins, which are internal components, due to the effects of aging and external damage can be cited (Moribe et al., Development of a hair internal repair technique using basic amino acids, J. Soc. Cosmet. Chem. Jpn., 50(2)98 - 103(2016), Baba et al., Composition change and elution of amino acids in hair by treatment with hair beauty materials, The Journal of Hygienic Chemistry, 19(2)47 - 52(1973)). Since hair does not have a self-repair function, efficient penetration of amino acids and proteins from the outside is desired for the repair of the inside of hair. However, since the hair surface is covered with fatty acids contained slightly in the hair itself, it has been reported that the permeability of useful components into the hair is low (Murakoshi Noriyuki, Structure and damage of hair seen by fluorescence, J. Soc. Cosmet. Chem. Jpn., 49(2)87 - 94(2015)), and provision of a component penetration technique for hair is desired.

[0004] Patent Document 1 reports that glycolic acid, xylitol, and / or adenosines have the effect of increasing the amount of glyoxylic acid that penetrates into the hair and the amount of glyoxylic acid remaining on the hair surface. However, this is intended for the penetration of acidic compounds as a perming agent for straightening curly or frizzy hair, and not for the penetration of amino acids or proteins. [Prior art documents] [Patent Documents]

[0005] [Patent Document 1] Japanese Patent Publication No. 2023-001087 [Overview of the project] [Problems that the invention aims to solve]

[0006] The present invention aims to provide an agent that promotes the penetration of amino acids into hair, a method for promoting the penetration of amino acids into hair, and the like. [Means for solving the problem]

[0007] As described above, efficient penetration of amino acids and proteins from the outside is desirable for repairing the inside of hair. Therefore, the inventors diligently conducted research to achieve the above objective and verified the penetration of basic amino acids that can be expected to repair hair using enzyme-treated royal jelly. By immersing healthy hair and damaged hair in the above material and quantifying the amino acids in the hair after washing, the penetration of amino acids was compared. The results showed that enzyme-treated royal jelly showed approximately 3.5 to 7.8 times higher penetration of arginine into hair and approximately 11.0 to 15.7 times higher penetration of lysine into hair compared to untreated royal jelly. Thus, it was found that enzyme-treated royal jelly can promote the penetration of amino acids into hair.

[0008] Based on these findings and further research, the present invention provides the following: an agent for promoting the penetration of amino acids into hair, a method for promoting the penetration of amino acids into hair, and the like.

[0009] Item 1: An enzyme-treated royal jelly containing an amino acid penetration enhancer for hair. Item 2: The osmotic accelerator according to Item 1, wherein the enzyme treatment is a protease treatment. Item 3: The penetration enhancer according to item 1 or 2, wherein the amino acid is a basic amino acid. Item 4: The osmotic agent according to item 3, wherein the basic amino acid is arginine and / or lysine. Item 5: The penetration enhancer according to any one of items 1 to 4, wherein the enzyme-treated royal jelly is an enzyme-treated product of royal jelly obtained by heating royal jelly to 60°C and treating it with enzymes under high pressure conditions of 50 MPa. Item 6: A penetration enhancer described in any one of items 1 to 5, which is a cosmetic or quasi-drug. Item 7: A method for promoting the penetration of amino acids into hair, comprising the step of applying a penetration enhancer described in any one of items 1 to 6 to the hair. [Effects of the Invention]

[0010] Enzyme-treated royal jelly is useful as an active ingredient in hair hair penetration enhancers for amino acids (especially basic amino acids such as arginine and lysine). [Brief explanation of the drawing]

[0011] [Figure 1] This graph shows the penetration rate (%) of amino acids (arginine) in royal jelly, enzyme-treated royal jelly A, and enzyme-treated royal jelly B into hair. [Figure 2] This graph shows the penetration rate (%) of the amino acid (lysine) in royal jelly, enzyme-treated royal jelly A, and enzyme-treated royal jelly B into hair. [Modes for carrying out the invention]

[0012] The following describes in detail embodiments for carrying out the present invention, but the present invention is not limited to the following embodiments.

[0013] The present invention's amino acid penetration enhancer for hair is characterized by containing enzyme-treated royal jelly as an active ingredient (hereinafter sometimes referred to as "the penetration enhancer of the present invention").

[0014] The present invention provides a method for promoting the penetration of amino acids into hair, characterized by comprising the step of applying the penetration enhancer to the hair.

[0015] The enhanced penetration of amino acids into hair means that a greater amount of amino acids penetrates the hair compared to cases where enzyme-treated royal jelly is not included (for example, cases where untreated royal jelly is included). The amino acids that enhance penetration into hair in the present invention are not particularly limited, but basic amino acids are preferred, and examples of basic amino acids include arginine, lysine, histidine, and tryptophan, with arginine and lysine being particularly preferred. The amino acids that enhance penetration into hair may be amino acids contained in enzyme-treated royal jelly, or amino acids added to enzyme-treated royal jelly. The enhanced penetration of amino acids into hair can be evaluated, for example, by the method shown in the examples, which involves immersing hair in enzyme-treated royal jelly for a certain period of time and then analyzing the amino acids in the enzyme-treated royal jelly that have penetrated the hair.

[0016] By using the amino acid penetration enhancer for hair of the present invention, the amount of amino acids that penetrate the hair becomes, for example, twice as much, preferably 2.5 times as much, and more preferably three times as much, compared to using untreated royal jelly. The hair that the penetration enhancer of the present invention promotes amino acid penetration of is particularly scalp hair. Furthermore, the hair may be healthy or damaged by perms, dyeing, etc.

[0017] Royal jelly is a milky jelly-like substance made by mixing the secretions secreted from the hypopharyngeal gland and the major salivary gland of worker bees aged 3 to 12 days. The main bioactive components in royal jelly include, for example, organic acids such as 10-hydroxy-2-decenoic acid (hereinafter referred to as "decenoic acid") peculiar to royal jelly, as well as proteins, lipids, saccharides, vitamins B, folic acid, nicotinic acid, pantothenic acid and other vitamins, various minerals, and the like. The production area of royal jelly may be any of European countries, Oceanian countries, the United States, Brazil, Japan, China, and other Asian countries, etc.

[0018] Enzyme-treated royal jelly is preferably royal jelly treated with a proteolytic enzyme (protease). Preferably, it is a low-allergen enzyme-treated royal jelly in which an allergic reaction caused by the protein contained in royal jelly is suppressed by protease treatment. Therefore, the components contained in enzyme-treated royal jelly include, in addition to the protease degradation products of the proteins contained in royal jelly, organic acids such as decenoic acid described above, lipids, saccharides, vitamins, and various minerals.

[0019] The royal jelly used for the production of enzyme-treated royal jelly is not particularly limited, and examples thereof include raw royal jelly, royal jelly powder obtained by drying raw royal jelly into powder, or a product obtained by extracting raw royal jelly with water or hydrous ethanol, etc. <了

[0020] The production of enzyme-treated royal jelly can be carried out by treating royal jelly raw material with an enzyme having at least endopeptidase activity, an enzyme having at least exopeptidase activity, and / or an enzyme having both endopeptidase activity and exopeptidase activity.

[0021] Examples of proteolytic enzymes having at least endopeptidase activity include endopeptidases derived from animals (e.g., trypsin, chymotrypsin, etc.), plants (e.g., papain, etc.), and microorganisms (e.g., lactic acid bacteria, yeast, mold, Bacillus subtilis, actinomycetes, etc.).

[0022] Examples of proteolytic enzymes having at least exopeptidase activity include carboxypeptidase, aminopeptidase, exopeptidases derived from microorganisms (e.g., lactic acid bacteria, Aspergillus spp., Rhizopus spp., etc.), pancreatin having endopeptidase activity, pepsin, etc.

[0023] Among these various enzymes, preferred examples of enzymes possessing both exopeptidase activity and endopeptidase activity include Streptomyces griseus-produced peptidase (product name: Actinase AS), Aspergillus oryzae-produced peptidase (product names: Protease A, Flavorzyme, Proteax), and Aspergillus melleus-produced peptidase (product name: Protease P). Preferred examples of enzymes possessing exopeptidase activity include Aspergillus oryzae-produced peptidase (product names: Umamizyme G, Promod 192P, Promod 194P, Sumizyme FLAP), and Aspergillus soe (Aspergillus Examples include peptidases produced by *Aspergillus sojae* (product name: Sternzyme B15024), peptidases produced by *Aspergillus* species (product name: Cochlase P), and peptidases produced by *Rhizopus oryzae* (product name: Peptidase R). Furthermore, preferred examples of enzymes possessing endopeptidase activity include peptidase produced by Bacillus subtilis (trade names: Orientase 22BF, Nucleicin), peptidase produced by Bacillus licheniformis (trade name: Alcalase), peptidase produced by Bacillus stearothermophilus (trade name: Protease S), peptidase produced by Bacillus amyloliquefaciens (trade name: Neutralase), and peptidase produced by Bacillus species (trade name: Protamex).

[0024] The amount of proteolytic enzyme used in the royal jelly reaction can be appropriately determined considering the concentration of the royal jelly, the type of enzyme, the reaction temperature, and the reaction time. The proteolytic enzyme may be added to the royal jelly all at once, or in small amounts in separate doses.

[0025] During proteolytic enzyme treatment, the pH is selected from a range of pH 2 to 12, preferably pH 7 to 10, and more preferably pH 7 to 8.5, corresponding to the optimal pH of the enzyme used. Specifically, before adding the proteolytic enzyme to the royal jelly solution or dispersion, the pH is adjusted to the desired range of pH 2 to 12, preferably pH 7 to 10, and more preferably pH 7 to 8.5, by adding an acid, alkali, or buffer, depending on the type of enzyme used. Examples of acids include hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, and acetic acid; examples of alkalis include sodium hydroxide, potassium hydroxide, and potassium carbonate; and examples of buffers include phosphate buffers and citrate buffers.

[0026] Royal jelly can be subjected to proteolytic enzyme treatment as is, or dissolved or dispersed in water. If it is in a dried form, it is preferable to subject it to proteolytic enzyme treatment while dissolved in water.

[0027] The temperature for proteolytic enzyme treatment is not particularly limited and is selected from a range that is practical and includes the optimal temperature range in which protease activity is expressed, i.e., usually from 20 to 70°C. Preferably, it is in the range of 40 to 70°C.

[0028] In the present invention, it is preferable to carry out the above-described proteolytic enzyme treatment under high pressure conditions. Examples of high pressure conditions include 50 MPa and a temperature of 60°C.

[0029] Furthermore, such high-pressure processing can be carried out using any conventionally known method, particularly known methods or known high-pressure processing equipment used for high-pressure processing of food products. Specifically, such high-pressure processing methods include, but are not limited to, a method of pressurizing a liquid that serves as a pressure medium by pumping it into a high-pressure chamber of limited volume (pump pressurization method), or a method of reducing the internal volume of the high-pressure chamber by pushing a piston into it (piston pressurization method).

[0030] The proteolytic enzyme treatment is stopped by inactivating or removing the proteolytic enzyme. Inactivation can be easily performed by heat treatment (for example, at 85°C for 15 minutes).

[0031] Enzyme-treated royal jelly includes concentrated liquids obtained by concentrating the enzyme-treated product, solids obtained by removing the solvent from the enzyme-treated product through freeze-drying, spray-drying, etc., powders obtained by pulverizing the enzyme-treated product, and granular forms obtained by granulating the powder.

[0032] Other known components may be appropriately incorporated into the penetration enhancer of the present invention, as long as they do not interfere with the effects of the present invention.

[0033] The content of enzyme-treated royal jelly in the penetration enhancer of the present invention is not particularly limited as long as the effects of the present invention can be obtained, and can be appropriately adjusted according to the final form, etc., relative to the total amount of solids in the penetration enhancer, for example, 0.00001% by mass or more, 0.0001% by mass or more, 0.001% by mass or more, 0.01% by mass or more, 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 3% by mass or more, 5% by mass or more, 7% by mass or more, 10% by mass or more, 15% by mass or more, 20% by mass or more , 30% by mass or more, 40% by mass or more, 50% by mass or more, 60% by mass or more, 70% by mass or more, 80% by mass or more, or 90% by mass or more, 99.9% by mass or less, 90% by mass or less, 80% by mass or less, 70% by mass or less, 60% by mass The following may be 55% by mass or less, 50% by mass or less, 40% by mass or less, 30% by mass or less, 20% by mass or less, 15% by mass or less, 10% by mass or less, 7% by mass or less, 5% by mass or less, 3% by mass or less, 1% by mass or less, or 0.5% by mass or less.

[0034] The dosage of the penetration enhancer of the present invention may vary depending on the active ingredient, the site of application, and the scope of application. For example, the amount of enzyme-treated royal jelly applied to hair is, for example, 0.01 mg to 50 mg, preferably 0.02 mg to 40 mg, and more preferably 0.05 mg to 35 mg, in terms of dry solid content.

[0035] The penetration enhancer of the present invention may be administered once a day, or divided into multiple doses, such as twice a day or three times a day, as long as the effective daily dose is within the range described above. The composition of the present invention provides immediate effects after administration, but continuous administration for 1 to 4 weeks, 1 month or more, 6 months or more, or 1 year or more is preferable because it allows for a longer-lasting effect.

[0036] The penetration enhancer of the present invention can be used as a cosmetic (for hair), a quasi-drug, etc. Furthermore, the penetration enhancer of the present invention also encompasses the meaning of an additive that imparts an effect of promoting the penetration of amino acids into hair.

[0037] In addition to the enzyme-treated royal jelly mentioned above, the above-mentioned cosmetics may contain, as needed, other ingredients commonly used in hair cosmetics, such as UV absorbers, disinfectants, preservatives, surfactants, alcohols, aqueous components, water, colorants, pH adjusters, blood circulation promoters, solubilizers, enzymes, chelating agents, thickeners, bases, emulsifiers, solvents, stabilizers, oils, etc.

[0038] Cosmetic formulations can take a wide range of forms, including aqueous solutions, solubilized solutions, emulsified solutions, powders, oil-based solutions, gels, ointments, aerosols, two-layer water-oil systems, and three-layer water-oil-powder systems.

[0039] The uses of cosmetics are also arbitrary, and examples include shampoo, rinse, hair treatment, hair conditioner, hair styling products, hair tonic, hair dye, hair manicure, pomade, hair liquid, hair cream, hair spray, hair mist, hair milk, hair growth products, etc.

[0040] In one embodiment, the penetration enhancer (cosmetic) of the present invention can be produced by heating royal jelly to 60°C and enzymatically treating it under high pressure conditions of 50 MPa, and then incorporating the enzyme-treated royal jelly into a cosmetic composition.

[0041] Furthermore, the cosmetics of this invention also include quasi-drugs.

[0042] The penetration enhancer of the present invention, as described above, is applicable to mammals, including humans (preferably humans).

[0043] The penetration enhancer of the present invention is based on enzyme-treated royal jelly and exhibits improved amino acid penetration into hair compared to untreated royal jelly. Therefore, enzyme-treated royal jelly is useful as an active ingredient in amino acid penetration enhancers for hair.

[0044] In this specification, "comprise" encompasses both the meanings of "essentially consist of" and "consist of." [Examples]

[0045] The present invention will be described more specifically below based on examples. However, the present invention is not limited to the following examples.

[0046] Test Example 1 <Royal Jelly Sample> • Royal jelly (fresh royal jelly) • Enzyme-treated royal jelly A (Royal jelly that has been enzyme-treated under high temperature and pressure (60°C, 50MPa) from raw royal jelly) • Enzyme-treated royal jelly B (royal jelly prepared from raw royal jelly according to the method described in International Publication No. 2020 / 196065A1)

[0047] <Hair Sample> • Royal jelly immersion treatment (3 types) • Perming treatment → Royal jelly immersion treatment (3 types)

[0048] <Experimental Method> Homogenization of hair samples 1. Hair from the same person (Beaulux Co., Ltd.) was cut with scissors every 10 cm from the root, and the ends were set aside. 2. Place the mixture in a polyethylene bag and shake to mix. 3. Place the mixture on a tray, cut it to the same length, divide it into 1g portions, and tie one end together with a rubber band.

[0049] Perming treatment 1. Untreated hair was immersed in perm solution 1 (active ingredients: ammonium thioglycolate 6.75%, monoethanolamine 1.00%, ammonia 0.35%) at room temperature for 15 minutes in a bath ratio of 1:6 (hair:perm solution ratio (w / w)). 2. After rinsing with running water for 1 minute, the hair was immersed in perm solution 2 (active ingredient: sodium bromate 7%) at room temperature for 15 minutes in a bath ratio of 1:6 (hair:perm solution ratio (w / w)). 3. After rinsing with running water for 1 minute, the item was dried with a hairdryer.

[0050] RJ immersion treatment 1. Hair samples were immersed in royal jelly samples at a bath ratio of 1:5 (hair:royal jelly ratio (w / w)) for 15 minutes. 2. After rinsing with running water for 1 minute, the item was dried with a hairdryer.

[0051] Amino acid measurement 1. Half of each strand of hair was cut below the rubber band and ground in a mill mixer. 2. 5 mg of hair sample and 5 zirconia beads (φ1 mm) were placed in a 1.5 mL Eppendorf tube, and 250 μL of methanol was added. 3. Stirring was performed using a vortex mixer for 10 minutes. 4. After stirring, 100 μL of purified water and 250 μL of chloroform were added. 5. Stirred using a vortex mixer for 30 seconds. 6. After stirring, the mixture was centrifuged at 3000 rpm for 5 minutes. 7. The water-methanol layer (upper layer) was transferred to a 2 mL cap-lock tube. 8. 100 μL of water was added to the chloroform layer (lower layer). Liquid-liquid extraction of 9.5-7 was performed twice (a total of three extractions). 10. 400 μL of ethanol was added to the recovered water-methanol layer. The solvent was removed using a spray device set to 11.40°C. 12. The dried material was redissolved in 100 μL of water, filtered, and the resulting solution was analyzed by LC-MS / MS.

[0052] Analysis conditions (LC-MS / MS) Liquid chromatograph: Ultimate 3000 (manufactured by Thermo Fisher Scientific) Mass spectrometer: Q Exactive Focus (manufactured by Thermo Fisher Scientific) Column: Discovery HS F5 (4.6 × 250 mm, 5 μm, manufactured by Sigma-Aldrich) Mobile phase: 0.1% Formic acid / Acetonitrile Flow rate: 0.5mL / min Column temperature: 40℃

[0053] <Result> Healthy hair and damaged hair (healthy hair that had been permed) were immersed in royal jelly, enzyme-treated royal jelly A, or enzyme-treated royal jelly B, and the amount of amino acids in the hair was examined. The penetration rates (%) of the basic amino acids arginine and lysine are shown in Table 1, Figures 1 and 2. The penetration rate here is expressed as a percentage (%) of the amount of amino acids detected in the hair relative to the amount of amino acids in the royal jelly used for the immersion treatment.

[0054] Enzyme-treated royal jelly A or B showed approximately 3.5 or 7.8 times higher arginine penetration into hair, and approximately 11.0 or 15.7 times higher lysine penetration, respectively, compared to untreated royal jelly. Furthermore, it was confirmed that damaged hair showed approximately 2.0 or 1.6 times higher arginine penetration, and approximately 2.5 or 2.2 times higher lysine penetration, respectively, compared to healthy hair.

[0055] [Table 1]

Claims

1. An enzyme-treated royal jelly-containing agent that enhances the penetration of amino acids into the hair.

2. The penetration enhancer according to claim 1, wherein the enzyme treatment is a protease treatment.

3. The penetration enhancer according to claim 1, wherein the amino acid is a basic amino acid.

4. The penetration enhancer according to claim 3, wherein the basic amino acid is arginine and / or lysine.

5. The penetration enhancer according to claim 1, wherein the enzyme-treated royal jelly is an enzyme-treated royal jelly product obtained by heating royal jelly to 60°C and treating it with an enzyme under high pressure conditions of 50 MPa.

6. A penetration enhancer according to any one of claims 1 to 5, which is a cosmetic or quasi-drug.

7. A method for promoting the penetration of amino acids into hair, comprising the step of applying a penetration enhancer according to any one of claims 1 to 5 to the hair.

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