cosmetic ingredients

Abstract

To provide a cosmetic composition that can promote skin cell turnover and enhance the skin's barrier function by more effectively activating epidermal keratinocytes. [Solution] The cosmetic composition is characterized by containing ceramides and specific components such as at least one sweetener selected from aspartame, reduced palatinose, and reduced maltose as active ingredients.

Description

Technical Field

[0001] The present invention relates to a cosmetic composition, and more particularly to a cosmetic composition containing ceramides and specific other components as active ingredients.

Background Art

[0002] The stratum corneum of the skin is present on the outermost shell of the skin and performs the barrier function of the skin. The barrier function of the skin is a function of suppressing the amount of transdermal water evaporation to retain the moisture of the skin and preventing the invasion of foreign substances from the outside into the living body. If this skin barrier function is not sufficiently exerted, dry skin, rough skin, dermatitis, etc. will occur. In the skin, in order to exert this barrier function, the skin (epidermis) performs metabolism, that is, turnover.

[0003] However, when the skin is affected by external factors such as ultraviolet rays or when aging progresses, the activity and proliferation ability of epidermal cells decrease, and accordingly, the skin turnover rate delays.

[0004] Therefore, improving and enhancing the skin barrier function has become one of the important issues from a cosmetic point of view.

[0005] As pharmaceuticals and cosmetics for improving and enhancing such skin barrier functions, for example, a skin barrier function enhancer containing one or more selected from angelica and its extracts (see Patent Document 1), a skin barrier function enhancer containing one or more selected from carrot and its extracts (see Patent Document 2), a cosmetic for improving skin barrier function containing any one or more selected from (A) nicotinamide, calcium pantothenate, and sage extract, (B) menthol esters of fatty acids, and (C) any one or more selected from diisopropyl sebacate, menthoxypropanediol, and bisethoxydiglycol cyclohexane-1,4-dicarboxylate (see Patent Document 3), etc. have been proposed.

Prior Art Documents

Patent Documents

[0006] [Patent Document 1] Japanese Patent Publication No. 2015-105269 [Patent Document 2] Japanese Patent Publication No. 2015-105268 [Patent Document 3] Japanese Patent Publication No. 2013-184936 [Overview of the project] [Problems that the invention aims to solve]

[0007] The object of the present invention is to provide a cosmetic composition that can promote skin cell turnover and enhance the skin's barrier function by more effectively activating epidermal keratinocytes. [Means for solving the problem]

[0008] The inventors of this invention diligently investigated and researched the activation of epidermal keratinocytes in order to enhance the barrier function of the skin. They discovered that a high epidermal keratinocyte activation effect can be obtained by combining ceramides with certain other components, and thus completed the present invention. In other words, they found that epidermal keratinocytes can be synergistically activated by combining ceramides with certain other components that have little to no epidermal keratinocyte activation ability.

[0009] In other words, the present invention relates to a cosmetic composition characterized by containing ceramides and at least one component selected from the group consisting of (a) to (d) below as active ingredients. (a) at least one sweetener selected from aspartame, reduced palatinose, and reduced maltose (b) At least one amino acid selected from cysteine, leucine, lysine, methionine, and tyrosine. (c) At least one mineral selected from iron and molybdenum (d) At least one cosmetic ingredient selected from collagen and proteoglycans.

[0010] The cosmetic composition of the present invention has a skin barrier function improving and enhancing effect and can be used as a skin barrier function improving and enhancing agent for improving and enhancing the skin's barrier function.

[0011] The cosmetic composition of the present invention may be obtained by adding an active ingredient consisting of ceramides and at least one component selected from the group consisting of (a) to (d).

[0012] Furthermore, the cosmetic composition of the present invention is preferably for oral use, and is preferably in the form of tablets, capsules, powders, granules, or liquids.

[0013] Furthermore, the present invention relates to a beauty method (excluding medical procedures) characterized by ingesting the beauty composition of the present invention described above. [Effects of the Invention]

[0014] According to the cosmetic composition of the present invention, by more effectively activating epidermal keratinocytes, skin turnover can be promoted and the skin's barrier function can be more effectively enhanced. [Brief explanation of the drawing]

[0015] [Figure 1] This figure shows the cell-activating activity rate when the cosmetic composition of the present invention (ceramides + sweetener) is applied to normal human epidermal keratinocytes (NHEK). The left figure shows the case using aspartame as the sweetener, and the right figure shows the case using reduced palatinose as the sweetener. [Figure 2] This figure shows the cell-activating activity rate when the cosmetic composition of the present invention (ceramides + sweetener) is applied to normal human epidermal keratinocytes (NHEK), and the figure shows the case in which reduced maltose is used as the sweetener. [Figure 3]It is a diagram showing the cell activation activity rate when the cosmetic composition of the present invention (ceramides + amino acids) is applied to normal human epidermal keratinocytes (NHEK). The left diagram shows the case where cysteine is used as the amino acid, and the right diagram shows the case where leucine is used as the amino acid. [Figure 4] It is a diagram showing the cell activation activity rate when the cosmetic composition of the present invention (ceramides + amino acids) is applied to normal human epidermal keratinocytes (NHEK). The left diagram shows the case where lysine (lysine hydrochloride) is used as the amino acid, and the right diagram shows the case where methionine is used as the amino acid. [Figure 5] It is a diagram showing the cell activation activity rate when the cosmetic composition of the present invention (ceramides + amino acids) is applied to normal human epidermal keratinocytes (NHEK), showing the case where tyrosine is used as the amino acid. [Figure 6] It is a diagram showing the cell activation activity rate when the cosmetic composition of the present invention (ceramides + minerals) is applied to normal human epidermal keratinocytes (NHEK). The left diagram shows the case where iron (iron chloride) is used as the mineral, and the right diagram shows the case where molybdenum (molybdenum chloride) is used as the mineral. [Figure 7] It is a diagram showing the cell activation activity rate when the cosmetic composition of the present invention (ceramides + cosmetic components) is applied to normal human epidermal keratinocytes (NHEK). The left diagram shows the case where collagen is used as the cosmetic component, and the right diagram shows the case where proteoglycan is used as the cosmetic component.

Mode for Carrying Out the Invention

[0016] The cosmetic composition of the present invention is characterized by containing ceramides and at least one component selected from the group consisting of the following (a) to (d) (hereinafter sometimes referred to as other components) as active ingredients. The components of the following (a) to (d) used together with ceramides may be used alone (for example, component (a)) or in combination of two or more. When using two or more other components in combination, it is preferable to combine other components having a high synergistic effect with ceramides.

[0017] The cosmetic composition of the present invention can more effectively activate epidermal keratinocytes, thereby promoting skin cell turnover and more effectively enhancing the skin's barrier function. The cosmetic composition of the present invention has effects such as improving and enhancing skin barrier function, promoting skin cell turnover, and activating epidermal keratinocytes. Therefore, the cosmetic composition of the present invention can be used as a skin barrier function improving and enhancing agent, a skin cell turnover promoting agent, and an epidermal keratinocyte activator.

[0018] [Ceramides] The ceramides used in this invention include human-type ceramides such as ceramide 1, ceramide 2, and ceramide 3, as well as animal-derived ceramides extracted from the brains and spinal cords of animals such as cattle, horses, and pigs, and plant-derived ceramides extracted from wheat, rice, soybeans, spinach, etc. Furthermore, the ceramides used in this invention may also be glycoceramides, specifically those with monosaccharides such as galactosylceramide and glucosylceramide, or those with oligosaccharides.

[0019] [Other ingredients] (a) Sweeteners In the cosmetic composition of the present invention, it is preferable to use at least one sweetener selected from aspartame, reduced palatinose, and reduced maltose as an active ingredient, along with ceramides. The sweetener of the present invention refers to any substance that exhibits sweetness and is not limited to its intended use as a sweetener.

[0020] (b) amino acids In the cosmetic composition of the present invention, it is preferable to use at least one amino acid (including a salt) selected from cysteine, leucine, lysine, methionine, and tyrosine as an active ingredient, along with ceramides. Examples of salts include sodium salts and hydrochloride salts.

[0021] (c) Minerals In the cosmetic composition of the present invention, it is preferable to use at least one mineral selected from iron and molybdenum as an active ingredient, along with ceramides. Note that the iron and molybdenum minerals in the present invention include compounds containing iron and molybdenum, respectively.

[0022] (d) Beauty ingredients In the cosmetic composition of the present invention, it is preferable to use at least one cosmetic ingredient selected from collagen and proteoglycans as an active ingredient, along with ceramides.

[0023] The collagen may be animal-derived or synthetic, and may include collagen protein, collagen peptides obtained by hydrolyzing collagen protein, and atelocollagen obtained by treating collagen molecules with protease and removing the telopeptide portion. Among animal-derived collagen, fish-derived collagen is particularly preferred. The average molecular weight (weight-average molecular weight) of the collagen is not particularly limited, but is preferably 500 to 100,000, and more preferably 1,000 to 50,000.

[0024] Proteoglycans are complex polysaccharides in which glycosaminoglycans such as chondroitin sulfate and dermatan sulfate are covalently bonded to a protein core, and they are abundant in animal tissues, especially cartilage tissue. In vivo, proteoglycans are also known to exist in a structure in which the core protein is further bound to hyaluronic acid, and their molecular weight is large, ranging from tens of thousands to tens of millions. Specifically, the proteoglycans used in this invention can be animal-derived proteoglycans, and commercially available products can be used.

[0025] The beauty composition of the present invention contains ceramides and other specified components, and is not particularly limited as long as it can be distinguished from other products as a product in that it is used for beauty purposes. For example, any product in which the main body, packaging, instructions, or promotional materials of the present invention display a statement that it has beauty effects such as improving the skin barrier function, promoting skin turnover, and activating epidermal keratinocytes is included in the scope of the present invention. Examples include pharmaceuticals (including quasi-drugs), cosmetics, and so-called health foods such as functional foods whose efficacy has been approved by a designated institution, such as Foods for Specified Health Uses, Foods with Nutrient Function Claims, and Foods with Function Claims. Examples of so-called health foods include those that display statements such as "barriers the skin," "protects the skin," "enhances the skin barrier function," and "enhances the skin's moisturizing power." The beauty composition of the present invention can be taken by anyone who is interested in the above effects, regardless of gender or age, but it is preferable for women to take it, more preferably for women in their 30s and older, and especially preferably for postmenopausal women, in order to more effectively enjoy the effects of the beauty composition of the present invention.

[0026] The cosmetic composition of the present invention can be used orally or externally. There are no particular limitations on the external preparation, as long as it is applied to the skin, scalp, etc. Examples of external preparations include ointments, creams, gels, lotions, emulsions, packs, patches, and other skin preparations, as well as injections.

[0027] Furthermore, when the beauty composition of the present invention is used as an oral preparation, examples of its forms include tablets, capsules, powders, granules, liquids, granular preparations, rod-shaped preparations, plate-shaped preparations, block-shaped preparations, solid preparations, round preparations, paste-like preparations, cream-like preparations, caplet-like preparations, gel-like preparations, chewable preparations, stick-shaped preparations, and the like. Among these, the forms of tablets, capsules, powders, granules, and liquid preparations are particularly preferred. Specifically, examples include supplements, beverages in containers such as PET bottles, cans, and glass bottles, and instant powdered beverages and instant granular beverages that are dissolved in water (hot water), milk, fruit juice, green juice, etc. These are preferred because they are easy to drink with meals and can enhance palatability.

[0028] The amount of ceramides and other components (active ingredients) in the cosmetic composition of the present invention may be appropriately included within a range that achieves their effects.

[0029] Generally, when the cosmetic composition of the present invention is a pharmaceutical or supplement (tablet, capsule), it is preferable that the active ingredient is present in an amount of 0.01 to 100% by mass of the total on a dry weight basis, more preferably 0.1 to 85% by mass, and even more preferably 0.5 to 70% by mass.

[0030] When the beauty composition of the present invention is a packaged beverage (liquid), it is preferable that the active ingredient is present in an amount of 0.01 to 15% by mass of the total on a dry weight basis, more preferably 0.03 to 12.5% ​​by mass, and even more preferably 0.05 to 10% by mass.

[0031] Furthermore, when the beauty composition of the present invention is an instant powder beverage (powder) or an instant granular beverage (granule), it is preferable that the active ingredient is present in an amount of 0.1 to 80% by mass of the total on a dry weight basis, more preferably 0.5 to 70% by mass, and even more preferably 1 to 60% by mass.

[0032] In order to more effectively exert the effects of the present invention, it is preferable that the active ingredient constitutes 80% by mass or more of the total beauty composition of the present invention (excluding water) on a dry weight basis, more preferably 90% by mass or more, even more preferably 95% by mass or more, and particularly preferably 100% by mass. Furthermore, if the beauty composition of the present invention contains any of components (a) to (d), it is preferable that the contained components consist only of the active ingredient of the present invention. That is, if the beauty composition of the present invention contains, for example, component (a) (sweetener), it is preferable that it is composed so as not to contain any sweeteners other than aspartame, reduced palatinose, and reduced maltose.

[0033] There are no particular restrictions on the amount of the cosmetic composition of the present invention to be ingested. However, from the viewpoint of exhibiting the effects of the present invention more significantly, it is preferable to ingest at least 0.5 mg / day of the active ingredient per day, more preferably at least 1 mg / day, and particularly preferably at least 1.5 mg / day. There is no particular upper limit, but for example, it is 4 g / day, and preferably 2 g / day.

[0034] The beauty composition of the present invention can be contained in one container, or divided into, for example, two or three containers, to represent one day's worth of intake, so that the daily intake amount is as described above.

[0035] The blending ratio of ceramides and other components is preferably in the range of 0.5:1 to 70:1, more preferably in the range of 0.75:1 to 60:1, even more preferably in the range of 1:1 to 60:1, and particularly preferably in the range of 1:1 to 50:1, based on dry weight. By blending the ceramides and other components within the above range, the effects of the present invention can be more effectively exhibited.

[0036] The cosmetic composition of the present invention can be manufactured by known formulation methods, with the addition of components other than the active ingredient that is acceptable as an oral or topical preparation, as needed.

[0037] Furthermore, as an oral cosmetic composition of the present invention, in addition to a cosmetic composition containing the active ingredient, other examples include cosmetic compositions obtained by adding the active ingredient to food (cosmetic foods). For example, these include food in which the content of the active ingredient of the present invention is increased compared to ordinary food (including natural food), and food in which the active ingredient of the present invention is added to food that does not normally contain the active ingredient of the present invention. The active ingredient may be added separately, simultaneously, or together with other ingredients other than the active ingredient.

[0038] Examples of beauty foods of the present invention include: beverages such as carbonated drinks, nutritional drinks, fruit drinks, lactic acid drinks, smoothies, and green juice; frozen desserts such as ice cream, ice sherbet, and shaved ice; noodles such as soba, udon, vermicelli, Chinese noodles, and instant noodles; confectionery such as candy, gum, chocolate, tablets, snacks, biscuits, jelly, jam, cream, baked goods, and bread; processed seafood and livestock products such as kamaboko, ham, and sausage; processed milk, fermented milk, and more. Examples include dairy products such as yogurt; oils and fats such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, and dressings, and their processed foods; seasonings such as sauces and soy sauce; and retort pouch foods such as curry, stew, oyakodon, porridge, rice gruel, chukadon, katsudon, tempuradon, gyudon, hayashi rice, omurice, oden, mapo tofu, gyoza, shumai, hamburgers, meatballs, various sauces, and various soups.

[0039] The present invention's beauty method is characterized by ingesting the beauty composition of the present invention as described above, but does not involve any medical procedures. It is preferable for women to ingest the beauty composition of the present invention, as this allows for more effective enjoyment of its effects; it is even more preferable for women in their 30s and older to ingest it; and it is particularly preferable for postmenopausal women to ingest it. Examples of the present invention's methods include providing the beauty food of the present invention in restaurants and other food service establishments to obtain beauty effects. Another example is providing the beauty food of the present invention as one of the treatments offered in aesthetic salons and the like. [Examples]

[0040] The present invention will be described below based on examples. [Example 1] 1.Cell culture (1) 75cm in a 37℃, 5% CO2 incubator 2 Normal human epidermal keratinocytes (NHEKs) were cultured in a special culture medium using flasks. (2) Cells suspended by trypsin treatment, 75 cm 2 From the flask, pour 1.0 × 10⁶ of liquid into each well of the 96-well plate. 4 Seeds were seeded at a cell density of cells / well. (3) Pre-cultured for 24 hours in a 37°C, 5% CO2 incubator. (4) After removing the culture medium from each well, 200 μL of culture medium containing the test substance, prepared to the specified concentration, was added to each well and incubated in a CO2 incubator for 48 hours.

[0041] For samples containing ceramides and other components, a culture medium containing the test substance at twice the concentration was prepared for each sample, and 100 μL of each was added to achieve the desired concentration. For the control samples, a 0.5% DMSO-containing medium was used for samples containing the test substance dissolved in DMSO, while a 0.25% DMSO medium was used for samples containing the other test substances.

[0042] For the ceramides, we used glucosylceramide derived from rice. Other components used in conjunction with the ceramides are shown in Tables 1 to 4.

[0043] 2. Measurement of cell activation activity (1) After 48 hours of incubation, the culture medium was removed from the cultured cells, washed once with 200 μL / well of PBS, and then 150 μL of Cell Counting Kit-8 solution diluted 30-fold with serum-free DMEM was added. (2) After being left to stand in a 37°C, 5% CO2 incubator to allow it to develop color appropriately, the absorbance at 450 nm was measured. (3) Based on the obtained data, the cell activation rate (% of control) was calculated.

[0044] % of control = (Data sample-Data blank) / (Data control-Data blank)×100

[0045] The results are shown in Tables 1 to 4 and Figures 1 to 7.

[0046] Each figure, from left to right, shows the results for "Control (no additives)," "Addition of ceramides alone," "Addition of other components alone," and "Addition of ceramides + other components." The numbers in the explanations below the graphs indicate the sample concentration; for example, "Gcel_1000" in the upper left graph of Figure 1 represents 1000 μg / mL of ceramides (glucosylceramide). The concentration unit for added components is μg / mL in all graphs. The vertical axis shows "% of control."

[0047] [Table 1]

[0048] [Table 2]

[0049] [Table 3]

[0050] [Table 4]

[0051] As shown in Tables 1 to 4 and Figures 1 to 7, combining ceramides with specific other components of the present invention synergistically increased epidermal keratinocyte activating activity. This indicates that epidermal keratinocytes proliferated. Therefore, the cosmetic composition of the present invention can promote skin cell turnover and more effectively enhance the skin's barrier function.

[0052] [Example 2] (Manufacturing of tablets) The following ingredients were mixed and compressed into tablets (300 mg). Take two of these tablets daily with water or another liquid.

[0053] weight ratio Reduced palatinose 40.0 Collagen peptide 25.0 Rice-derived glucosylceramide 3.3 Pig placenta 14.0 Cellulose 10.0 Niacin 2.0 Calcium stearate 3.2 Silicon dioxide 2.0 Vitamin B2 0.3 Vitamin B6 0.2

[0054] [Example 3] (Manufacturing of capsules) A mixture of the following ingredients was enclosed in soft capsules to produce capsules (600 mg). One capsule should be taken daily.

[0055] weight ratio Reduced maltose 20.0 Rice-derived glucosylceramide 4.0 Collagen peptide 25.0 Proteoglycan 25.0 Vitamin C 10.0 Cysteine ​​5.0 Methionine 5.0 Iron chloride 6.0

[0056] [Example 4] (Manufacture of granules) The following ingredients were mixed and a granular preparation (1200 mg) was manufactured by a conventional method.

[0057] weight ratio Aspartame 5.0 Rice-derived glucosylceramide 0.2 Cellulose 20.0 Calcium stearate 20.0 Collagen peptide 15.0 Proteoglycan 15.0 Vitamin C 10.0 Tyrosine 2.8 Leucine 2.0

[0058] [Example 5] (Manufacturing of instant powder formulation) The following ingredients were mixed and an instant powder (5000 mg) was manufactured by a conventional method.

[0059] weight ratio Aspartame 5.0 Rice-derived glucosylceramide 0.05 Cellulose 20.0 Calcium stearate 20.0 Collagen peptide 15.0 Proteoglycan 15.0 Vitamin C 10.0 Vitamin B1 4.0 Vitamin B2 3.0 Vitamin E 3.0 Tyrosine 2.8 Leucine 2.0

[0060] [Example 6] (Production of liquid formulation) A liquid preparation (50 mL) consisting of the following ingredients was manufactured.

[0061] weight ratio Reduced maltose 5.0 Aspartame 1.0 Rice-derived glucosylceramide 0.01 Collagen peptide 7.0 Proteoglycan 5.0 Vitamin C 2.0 Vitamin B1 0.5 Vitamin B2 0.5 Vitamin E 0.2 Tyrosine 0.1 Leucine 0.1 Lysine hydrochloride 1.0 water remainder [Industrial applicability]

[0062] The cosmetic composition of the present invention has high cosmetic efficacy and can be used as an oral or topical preparation, thus the industrial utility of the present invention is high.

Claims

[Claim 1] A cosmetic composition characterized by containing rice-derived glucosylceramide and at least one amino acid selected from cysteine, leucine, and methionine.

Images