Cosmetics and methods for producing extracts of the green alga Chlamydomonas

Abstract

We offer organic cosmetics containing at least one dipeptide derived from pig placenta. [Solution] The cosmetic contains an extract of the green alga Chlamydomonas. The extract of the green alga Chlamydomonas contains one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine.

Description

Technical Field

[0001] The present invention relates to cosmetics and a method for producing an extract of the green alga Chlamydomonas.

Background Art

[0002] Conventionally, placenta has attracted attention because it increases natural healing power. Here, glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine are known as dipeptides derived from porcine placenta.

[0003] Patent Document 1 describes a cosmetic composition containing a porcine placenta extract.

[0004] On the other hand, Patent Document 2 describes the Honda DREAMO strain (accession number FERM BP-22306) as an example of the green alga Chlamydomonas reinhardtii.

Prior Art Literature

Patent Literature

[0005]

Patent Document 1

Patent Document 2

Summary of the Invention

Problem to be Solved by the Invention

[0006] However, when porcine placenta is used, organic cosmetics cannot be produced.

[0007] An object of the present invention is to provide an organic cosmetic containing at least one dipeptide derived from porcine placenta.

Means for Solving the Problem

[0008] (1) A cosmetic comprising an extract of the green alga Chlamydomonas, wherein the extract of the green alga Chlamydomonas comprises one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine.

[0009] (2) The green alga Chlamydomonas is the Honda DREAMO strain (accession number FERM BP-22306), as described in (1).

[0010] (3) A method for producing an extract of the green alga Chlamydomonas, wherein the extract of the green alga Chlamydomonas contains one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine, and comprises the step of culturing the green alga Chlamydomonas while irradiating it with intermittent light.

[0011] (4) A method for producing an extract of the green alga Chlamydomonas as described in (3), wherein the irradiation time of the intermittent light per 24 hours is 8 hours or more and 10 hours or less. [Effects of the Invention]

[0012] According to the present invention, it is possible to provide an organic cosmetic product containing at least one dipeptide derived from porcine placenta. [Modes for carrying out the invention]

[0013] Embodiments of the present invention will be described below.

[0014] [cosmetics] The cosmetic of this embodiment contains an extract of the green alga Chlamydomonas. Here, the extract of the green alga Chlamydomonas contains one or more dipeptides selected from the group consisting of glycine-leucine (Gly-Leu), leucine-glycine (Leu-Gly), glycine-isoleucine (Gly-Ile), and isoleucine-glycine (Ile-Gly). As a result, an organic cosmetic containing at least one dipeptide derived from pig placenta is obtained. Consequently, it is expected that using the cosmetic of this embodiment will provide the same effects as a cosmetic containing pig placenta extract.

[0015] Examples of Chlamydomonas green algae include the UTEX 90 strain and the Honda DREAMO strain (accession number FERM BP-22306). Here, the UTEX 90 strain is the original strain from which the Honda DREAMO strain was derived.

[0016] Here, the extract of the Honda DREAMO strain contains Gly-Leu, Leu-Gly, and Ile-Gly.

[0017] The form of the cosmetic product in this embodiment is not particularly limited, but an example is a beauty serum. The beauty serum comprises, for example, a base material consisting of butylene glycol and water, and an extract of the green alga Chlamydomonas, wherein the content of the Chlamydomonas extract is 1 ppm or more and 10,000 ppm or less. Here, the ratio of butylene glycol to water is not particularly limited.

[0018] [Method for producing extract of the green alga Chlamydomonas] The method for producing an extract of the green alga Chlamydomonas according to this embodiment includes a step of culturing the green alga Chlamydomonas while irradiating it with intermittent light. As a result, the content of one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine is increased in the extract of the green alga Chlamydomonas.

[0019] The light period per 24 hours is preferably not less than 8 hours and not more than 10 hours, more preferably not less than 8.5 hours and not more than 9.5 hours. When the light period per 24 hours is not less than 8 hours and not more than 10 hours, the content of one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine and isoleucine-glycine in the extract of Chlamydomonas green algae increases.

[0020] The wavelength of light irradiated during the light period is not particularly limited, and is, for example, 300 nm or more and 800 nm or less. The photosynthetically active photon flux density of light irradiated during the light period is not particularly limited, and is, for example, 60 µmol / m 2 / s or more and 400 µmol / m 2 / s or less. The light source for irradiation during the light period is not particularly limited, and examples thereof include white LEDs.

[0021] The method for culturing Chlamydomonas green algae is not particularly limited, and examples thereof include static culture, shaking culture, deep culture, and aeration culture.

[0022] The medium used for culturing Chlamydomonas green algae is not particularly limited, and examples thereof include TAP medium, N1 medium, urea medium, HSM medium, and BG11 medium.

[0023] The temperature for culturing Chlamydomonas green algae is not particularly limited, and is, for example, 25°C or higher and 28°C or lower.

[0024] The gas aeration volume per unit volume when culturing Chlamydomonas green algae by aeration culture is not particularly limited, and is, for example, 0.001 VVM or more and 10 VVM or less. Here, VVM indicates how many times the volume of gas is aerated to the medium per minute, by volume ratio.

[0025] When culturing the green alga Chlamydomonas by aeration culture, it is preferable to supply carbon dioxide to the culture medium. The concentration of carbon dioxide in the gas being aerated is not particularly limited, but for example, it is between 0.1% and 10% by volume. The source of carbon dioxide is not particularly limited, but examples include carbon dioxide cylinders, gases emitted from factories, and concentrated carbon dioxide recovered from the atmosphere by DAC (Direct Air Capture).

[0026] Methods for recovering cultured green algae Chlamydomonas are not particularly limited, but examples include centrifugation and filtration.

[0027] The collected green algae Chlamydomonas may be dried if necessary.

[0028] Methods for drying the green alga Chlamydomonas are not particularly limited, but examples include freeze-drying, spray-drying, hot-air drying, and sun-drying.

[0029] While there are no particular limitations on the extraction method used for extracting the green alga Chlamydomonas, ultrasonic extraction is one example.

[0030] The solvent used for extracting the green alga Chlamydomonas is not particularly limited, but examples include lower alcohols such as methanol, ethanol, propyl alcohol, and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, and glycerin; and hydrophilic organic solvents such as dimethyl sulfoxide (DMSO). Among these, DMSO is preferred.

[0031] Methods for recovering the extract of the green alga Chlamydomonas are not particularly limited, but examples include centrifugation and filtration.

[0032] The recovered extract of the green alga Chlamydomonas may be dried if necessary.

[0033] There are no particular limitations on the method for drying the extract of the green alga Chlamydomonas, but one example is freeze-drying. [Examples]

[0034] The following describes embodiments of the present invention, but the present invention is not limited to these embodiments.

[0035] [Example 1] [Cultivation of the green alga Chlamydomonas] A portion of the Honda DREAMO strain, a type of green algae known as Chlamydomonas, was recovered from UREA medium and cultured in 6L of UREA medium for 3-4 days under the following conditions (seed culture). Temperature: 25~28℃ Light / dark cycle: 8-10 hours light period, 16-14 hours dark period Photosynthetic photon flux density (XP) of light irradiated during the light period: 60-400 μmol / m³ 2 / s Light source for illumination during the daytime: White LED Gas permeability per unit volume: 0.15 VVM Concentration of carbon dioxide in the gas: 5% by volume

[0036] Next, 500 mL of the seed culture was centrifuged at 3000 rpm for 5 minutes, then the culture supernatant was removed and the cultured Honda DREAMO strain was recovered.

[0037] Next, the recovered Honda DREAMO strain was cultured for 7 days with aeration (pre-culture) using 6L of UREA medium under the same culture conditions as the seed culture.

[0038] Next, using 1 L of N1 medium, the pre-cultured Honda DREAMO strain was subjected to aeration culture (main culture) for 7 days under the same culture conditions as the seed culture. The aeration conditions were as follows: Gas permeability per unit volume: 0.15 VVM Concentration of carbon dioxide in the gas: 5% by volume

[0039] Next, 5 L of the culture was centrifuged at 6000 rpm for 10 minutes, the culture supernatant was removed, and the cultured Honda DREAMO strain was recovered.

[0040] [extraction] Approximately 20 mL of the recovered Honda DREAMO strain was ultrasonically extracted with 100 mL of DMSO for 5 minutes using an ultrasonic homogenizer. The ultrasonic extraction was performed on ice.

[0041] Next, the extract and residue mixture was centrifuged at 3000 rpm for 5 minutes, the residue was removed, and approximately 100 mL of extract was recovered.

[0042] Next, the extract was freeze-dried to obtain an extract of the Honda DREAMO strain.

[0043] [Comparative Example 1] An extract of the Honda DREAMO strain was obtained in the same manner as in Example 1, except that continuous light was irradiated instead of intermittent light, i.e., the light period was set to 24 hours.

[0044] [Dipeptide content in extract of Honda DREAMO strain] High-performance liquid chromatography (HPLC) was used to measure the content [g] of dipeptides (Gly-Leu, Leu-Gly, and Ile-Gly) per gram (dry weight) of extract from the Honda DREAMO strain.

[0045] Table 1 shows the evaluation results of the dipeptide content in the extract of the Honda DREAMO strain.

[0046] [Table 1]

[0047] Table 1 shows that the extract of the Honda DREAMO strain in Example 1 has a higher dipeptide content than the extract of the Honda DREAMO strain in Comparative Example 1.

Claims

1. A cosmetic product containing an extract of the green alga Chlamydomonas, The aforementioned extract of the green alga Chlamydomonas contains one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine, and is a cosmetic product.

2. The cosmetic according to claim 1, wherein the green alga Chlamydomonas is the Honda DREAMO strain (accession number FERM BP-22306).

3. A method for producing an extract of the green alga Chlamydomonas, The extract of the green alga Chlamydomonas contains one or more dipeptides selected from the group consisting of glycine-leucine, leucine-glycine, glycine-isoleucine, and isoleucine-glycine. A method for producing an extract of the green alga Chlamydomonas, comprising the step of culturing the green alga Chlamydomonas while irradiating it with intermittent light.

4. A method for producing an extract of the green alga Chlamydomonas according to claim 3, wherein the irradiation time of the intermittent light per 24 hours is 8 hours or more and 10 hours or less.

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