Analytical methods, analytical devices, and analytical systems

Abstract

This provides an analytical method that can reduce the number of standard colors. [Solution] A liquid analysis method comprising: a preparation step of creating standard colors 71 to 74 that include the color composition values ​​of the first component at each concentration and the color composition values ​​of the second component at each concentration, by obtaining the value of one of the RGB color components (R value) for each concentration from a plurality of first component images 21a to 21d obtained by capturing images of the first component at two or more concentrations, and obtaining the value of one of the RGB color components (G value) for each concentration from a plurality of second component images 22a to 22d obtained by capturing images of the second component at two or more concentrations; and an analysis step of analyzing analytical image data 152 obtained by capturing the colored liquid and the standard colors 71 to 74, and analyzing the first component using the color composition value (R value) of the first component from the RGB values.

Description

[Technical Field]

[0001] The present invention relates to an analytical method, an analytical device, and an analytical system. [Background technology]

[0002] Conventionally, a method is known in which a reagent that exhibits a color reaction with the liquid to be analyzed is attached to a channel formed in a substrate, and the concentration of the analyte is analyzed using a color reaction. In Patent Document 1 below, aqueous solutions of ferrous ammonium sulfate, adjusted to different concentrations (0, 100, 250, 500, 1000 μM), are dropped onto an analytical device, and the resulting color reaction is analyzed by color analysis to measure the intensity of Green, thereby determining the Fe concentration in the sample solution. 2+ The difference in green intensity changes depending on the concentration, and therefore, Fe 2+ It is stated that the concentration can be quantified. [Prior art documents] [Patent Documents]

[0003] [Patent Document 1] Japanese Patent Publication No. 2016-50912 [Overview of the project] [Problems that the invention aims to solve]

[0004] Regarding the technology disclosed in Patent Document 1, if a certain component is colored at different concentrations and the resulting colors (standard colors) are obtained, the concentration of that component in a liquid can be analyzed using these standard colors. However, if standard colors are to be created for two or more components, a standard color will be needed for each component. Furthermore, for example, if multiple standard colors are colored onto a base sheet, and the concentration of a component in a liquid is analyzed by comparing the colored liquid with the standard colors on the base sheet, the number of components that can be analyzed may be limited by the space on the base sheet, depending on the number of standard colors.

[0005] The present invention relates to a technology completed based on the above circumstances, and provides an analytical method, analytical device, and analytical system that can reduce the number of standard colors. It also relates to providing an analytical method, analytical device, and analytical system that can suitably analyze multiple components. [Means for solving the problem]

[0006] The present invention provides an analytical method for a liquid containing at least one of a first component and a second component, comprising: a preparation step of creating a standard color that includes at least the color configuration values ​​of the first component and the color configuration values ​​of the second component, obtained from a plurality of first component images obtained by capturing the first component colored at two or more concentrations, and from a plurality of second component images obtained by capturing the second component colored at two or more concentrations, obtained from a plurality of second component images obtained by capturing the second component colored at two or more concentrations, and a standard color that includes at least the color configuration values ​​of the first component and the color configuration values ​​of the second component, obtained from at least the color configuration values ​​of the first component and the color configuration values ​​of the second component, respectively; and an analytical step of analyzing an analytical image obtained by capturing the colored liquid and the standard color, wherein (1) when analyzing the first component, the color configuration value of the first component is used from the RGB values, and (2) when analyzing the second component, the color configuration value of the second component is used from the RGB values.

[0007] Furthermore, the analytical device of the present invention is an analytical device for a liquid containing at least one of a first component and a second component, comprising: a first coloring section in which the first component exhibits color; a second coloring section in which the second component exhibits color; and a standard color section colored with a standard color, wherein the standard color is colored such that it includes at least: the values ​​for each concentration of any one color configuration among the RGB values ​​obtained from a plurality of first component images obtained by imaging the first component at two or more concentrations, and the values ​​for each concentration of any one color configuration different from the color configuration of the first component among the RGB values ​​obtained from a plurality of second component images obtained by imaging the second component at two or more concentrations.

[0008] Further, the analysis system of the present invention comprises: the above-mentioned analysis device; an imaging unit that captures an image of the colored liquid obtained in the analysis device and the standard color; and a control unit that analyzes the first component or the second component with respect to the analysis image captured by the imaging unit, wherein (1) when analyzing the first component, a color component value of the first component among RGB values is used, and (2) when analyzing the second component, a color component value of the second component among RGB values is used. Effects of the Invention

[0009] According to the present invention, it is possible to provide an analysis method, an analysis device, and an analysis system that can reduce the number of standard colors. It is also possible to provide an analysis method, an analysis device, and an analysis system that can suitably analyze a plurality of components. Brief Description of the Drawings

[0010] [Figure 1] Plan view showing the analysis device according to Embodiment 1 [Figure 2] Plan view showing a microfluidic device [Figure 3] Functional block diagram showing an analysis system [Figure 4] Flowchart showing a preparation step in the analysis method [Figure 5] Flowchart showing an analysis step in the analysis method [Figure 6] Flowchart showing a calculation step in the analysis step [Figure 7] Explanatory diagram showing the procedure of the preparation step [Figure 8] Explanatory diagram showing the procedure of the analysis step [Figure 9] Graph showing a calibration curve produced in the calculation step [Figure 10] Plan view showing the analysis device according to Embodiment 2 [Figure 11] Plan view showing the analysis device according to Embodiment 3 Mode for Carrying Out the Invention

[0011] <Embodiment 1> Embodiments of the present invention will be described in detail. The analysis method and the like described below are intended to embody the technical idea of the present invention, and unless otherwise specified, the present invention is not limited to the following contents.

[0012] As shown in FIG. 1, an analysis device 1 according to the present embodiment includes a microfluidic device 2 and a backing sheet 6 on which the microfluidic device 2 can be placed. The microfluidic device 2 is a member that exhibits a color reaction corresponding to a component in a liquid. For example, microfluidic Paper-based Analytical Devices (μPADs) in which a flow path for liquid analysis is provided on a paper substrate, or a device in which a flow path for liquid analysis is provided on a substrate such as glass or silicon can be employed.

[0013] In the present embodiment, "analysis" means quantitative analysis or semi-quantitative analysis such as the concentration of an analyte component. Also, in the present embodiment, "backing sheet" means a sheet-shaped member. That is, the backing sheet 6 is not limited to cardboard, and can be made of various materials such as thin paper, plastic plates, metal plates, plastic or metal sheets, for example. Furthermore, the backing sheet 6 may be a sticker whose back surface is glued and can be attached to a desk or the like.

[0014] The microfluidic device 2 includes a substantially circular introduction portion 3 onto which a liquid is dropped, and a plurality of flow paths 4, 5 connected to the introduction portion 3 and extending radially from an outer edge of the introduction portion 3. In each of the flow paths 4 and 5, a direction approaching the introduction portion 3 is defined as an upstream side (inner side), and a direction away from the introduction portion 3 is defined as a downstream side (outer side). Each of the flow paths 4 and 5 includes an analysis region 5 that exhibits a color reaction corresponding to a component in the liquid, and a time measurement region 4 whose color changes with the liquid. Each of the time measurement region 4 and the analysis region 5 includes a plurality of circular portions in plan view (an inner region, an intermediate region, and an outer region, which will be described later) and a linear flow path connecting these portions.

[0015] Methods for forming the channels 4 and 5 of the microfluidic device 2 include forming the channel walls on the substrate using a wax printer, or cutting the substrate into the shape of the channel.

[0016] A predetermined reagent is impregnated into a portion of the time measurement area 4 and the analysis area 5. Reagent impregnation can be performed by impregnating the predetermined area with a solution containing the dissolved reagent and then drying it. One type of reagent or multiple types may be impregnated into a single area. Furthermore, multiple types of reagents may be impregnated into a single area by repeating the impregnation and drying process multiple times.

[0017] As shown in Figure 2, the time measurement area 4 connected to the introduction section 3 has an inner area 4a to which a time measurement reagent is attached, and an outer area (time-measuring color-developing area) 4b located at the downstream end of the inner area 4a and to which no reagent is attached. The outer area 4b is configured to develop a predetermined color when liquid is dropped into the introduction section 3 and flows through the inner area 4a to the outer area 4b. The time measurement reagent is not particularly limited, and any reagent that exhibits color development in the outer area 4b can be used. For example, the time measurement reagent may be Acid Red 27, or it may be a reagent that exhibits a color reaction such as cobalt chloride. Note that color development may include cases where color develops at the attachment site where the reagent is attached, and cases where color develops at another location (non-attached location) after the liquid flows from the reaction site (for example, downstream).

[0018] As shown in Figure 1, the microfluidic device 2 of the analytical device 1 according to this embodiment has multiple analytical regions 5, each connected to the introduction section 3, and is configured to analyze multiple components contained in the liquid. Specifically, as shown in Figure 2, the analytical regions 5 include a pH analysis region 51 for analyzing pH, a Ca analysis region 52 for analyzing Ca (calcium), a NO2-N analysis region 53 for analyzing NO2-N (nitrite nitrogen), and PO4 3- ―PO4 for analyzing P (phosphorus) 3-—It comprises a region 54 for P analysis, a region 55 for NO3-N (nitrate nitrogen) analysis, and a region 56 for K (potassium) analysis. Below, for each component, PO4 3- It is sometimes written as the first component, NO2 as the second component, and NO3 as the third component. In addition, for each component, pH(H + Sometimes, ) is listed as the fourth component, Ca as the fifth component, and K as the sixth component.

[0019] The pH analysis region 51 has an inner region 51a and an outer region 51b located at the downstream end of the inner region 51a. Bromocresol green (BCG) is impregnated into the inner region 51a, and bromothymol blue (BTB) is impregnated into the outer region 51b.

[0020] The Ca analysis region 52 has an inner region 52a and an outer region 52b located at the downstream end of the inner region 52a. The inner region 52a and the outer region 52b are impregnated with ethylenediamine-N,N,N',N'-tetraacetate disodium salt dihydrate, o-cresolphthalein complexone, and 8-quinolinol, respectively.

[0021] The NO2-N analysis region 53 has an inner region 53a and an outer region 53b located downstream of the inner region 53a and without reagent impregnation. The inner region 53a is impregnated with citric acid monohydrate, trisodium citrate dihydrate, urea, and N-1-naphthylethylenediamine dihydrochloride, followed by L(+)-tartaric acid and sulfanilamide. The outer region 53b is an example of a second color-developing region, where the second component (NO2) reacts with the reagent impregnated in the inner region 53a and develops color.

[0022] PO4 3―The P analysis region 54 has an inner region 54a, an intermediate region 54b located downstream of the inner region 54a, and an outer region 54c located downstream of the intermediate region 54b. The inner region 54a is impregnated with trichloroacetic acid, ammonium heptamolybdate tetrahydrate, and citric acid monohydrate. The intermediate region 54b and the outer region 54c are impregnated with hydrochloric acid and stannous chloride, followed by tartrazine. By impregnating the same reagent separately in the intermediate region 54b and the outer region 54c in this way, even if a highly concentrated reagent is impregnated, the flow of the liquid is not obstructed, and the liquid reaches the end of the flow path, which is an advantage. The outer region 54c is an example of the first color-developing region, and the first component (PO4) reacts with the reagents impregnated in the intermediate region 54b and the outer region 54c. 3- This is the part that exhibits coloration.

[0023] The NO3-N analysis region 55 comprises an inner region 55a, an intermediate region 55b located downstream of the inner region 55a, and an outer region 55c located downstream of the intermediate region 55b. The inner region 55a is impregnated with urea, N-1-naphthylethylenediamine dihydrochloride, L(+)-tartaric acid, and sulfanilamide. The intermediate region 55b is impregnated with vanadium chloride. Furthermore, the outer region 55c is impregnated with citric acid monohydrate. The outer region 55c is an example of a third color-developing region, where the third component (NO3), which reacts with the reagents impregnated in the intermediate region 55b and the outer region 55c, exhibits color.

[0024] The K analysis region 56 has an inner region 56a and an outer region 56b located at the downstream end of the inner region 56a. The outer region 56b is impregnated with chromionophore I, valinomycin, potassium tetrakis(4-chlorophenyl)borate, and dioctyl sebacate.

[0025] The microfluidic device 2, having the above configuration, can analyze various liquids. Examples of such liquids include soil extracts and environmental water from rivers, lakes, and the sea.

[0026] It should be noted that each of the analysis regions 5 and the impregnated reagents described above are merely examples, and are not limited thereto.

[0027] As shown in Figure 1, the mounting sheet 6 includes: a device placement region 9 for placing the microfluidic device 2; a standard color portion 7 that is disposed outside the device placement region 9 (a part of the periphery of the device placement region 9) and colored with a plurality of standard colors; and markers 8 disposed at the end portions of the four corners of the mounting sheet 6. The mounting sheet 6 is configured such that a sample obtained by coloring a liquid to be analyzed (the microfluidic device 2 after color development) can be disposed in the vicinity of the standard color portion 7.

[0028] The microfluidic device 2 may be configured to be placed on the device placement region 9, may be configured to be detachably attached to the device placement region 9, or may be configured to be fixed to the device placement region 9. The number of microfluidic devices 2 relative to one mounting sheet 6 is not particularly limited. For example, one microfluidic device 2 may be used for one mounting sheet 6, or a plurality of microfluidic devices 2 may be used interchangeably for one mounting sheet 6.

[0029] The standard color portion 7 includes: a time measurement standard color 7a corresponding to the color developed in the time measurement region 4 of the microfluidic device 2; and component analysis standard color portions 7b and 7c colored with a plurality of standard colors (standard colors for component analysis) to be described later. The component analysis standard color portions 7b and 7c are sometimes called a color chart.

[0030] In a state where the microfluidic device 2 is disposed in the device placement region 9 (referred to as a placed state), a state where the colored liquid is disposed in the vicinity of the standard color portion 7 can be achieved. Specifically, in the placed state, the outer region 4b of the time measurement region 4, the outer region 51b of the pH analysis region 51, the outer region 52b of the Ca analysis region 52, and the outer region 56b of the K analysis region 56 are configured to be disposed in the vicinity of the time measurement standard color 7a and the upper component analysis standard color portion 7b. Furthermore, in the placed state, the outer region 53b of the NO2-N analysis region 53, PO4 3―The outer region 54c of the P analysis region 54 and the outer region 55c of the NO3-N analysis region 55 are arranged near the lower component analysis standard color section 7c. 3― —The outer region 54c of the P analysis region 54 is positioned closest to the lower component analysis standard color region 7c compared to the other analysis regions.

[0031] As shown in Figure 1, the lower component analysis standard color section 7c is composed of multiple (four in this embodiment) standard colors 71 to 74, each with a different color. The standard colors 71 to 74 include the first standard color 71, the second standard color 72, the third standard color 73, and the fourth standard color 74. Each of the standard colors 71 to 74 has a different RGB value. The respective RGB values ​​are determined in the preparation process described later (see Figure 7). The method of coloring the base paper 6 with the standard colors 71 to 74 is not particularly limited, but examples include inkjet printing, laser printing, offset printing, and thermal transfer printing. The upper component analysis standard color section 7b has the same configuration as the lower component analysis standard color section 7c, and its description is omitted.

[0032] The lower component analysis standard color section 7c is PO4 3― - The upper component analysis standard color section 7b is used to analyze the concentration of the first component that exhibits color in the outer region 54c of the P analysis region 54, the concentration of the second component that exhibits color in the outer region 53b of the NO2-N analysis region 53, and the concentration of the third component that exhibits color in the outer region 55c of the NO3-N analysis region 55.

[0033] In this embodiment, one marker 8 is provided at each of the four corners of the analysis device 1, but this is not limited to this configuration. Various arbitrary configurations can be adopted for the number and arrangement of the markers 8. Also, in this embodiment, the markers 8 are AR markers, and the measurement terminal 100 is configured to identify the area inside the four markers 8 as the marker-internal area of ​​the analysis device 1.

[0034] Next, the analysis system 110 of this embodiment will be explained using the functional block diagram shown in Figure 4. The analysis device 1 is one of the components of the analysis system 110. The analysis system 110 also includes a measurement terminal 100.

[0035] The measurement terminal 100 comprises a camera unit (imaging unit) 120, a display unit 130, a control unit 140, and a storage unit 150. The measurement terminal 100 may be, for example, a general-purpose terminal with a built-in camera such as a smartphone or tablet terminal, or it may be a computer such as a notebook PC or desktop PC with an external camera connected, or it may be a dedicated terminal for analysis of the analysis device 1.

[0036] The camera unit 120 includes at least one lens and one image sensor, and is configured to capture images of the analysis device 1. The camera unit 120 also has a video capture function and is configured to capture video of the microfluidic device 2 in the video capture processing described later, as shown in Figures 5 and 8. Furthermore, the camera unit 120 is configured to capture analysis image data (analysis image) 152 in the analysis image capture processing described later. Note that "capture" includes both capturing video and capturing still images.

[0037] In this embodiment, the measurement terminal 100 erases the video captured by the camera unit 120 without storing it in the storage unit 150, but is not limited to this, and the video may be stored in the storage unit 150 for saving. Also, in this embodiment, when the image acquisition process for analysis is repeatedly executed, the measurement terminal 100 is configured to overwrite and save all analysis image data 152 as needed without storing all of it in the storage unit 150, but is not limited to this, and all of the analysis image data 152 may be stored in the storage unit 150. Furthermore, the measurement terminal 100 may temporarily store the analysis image data 152 and delete it after the calculation of the analysis results 154 described later.

[0038] When imaging the analysis device 1, the user fixes the measurement terminal 100 (or the external camera if the camera unit 120 is an external camera) to an imaging jig such as a tripod so that the microfluidic device 2 and the mounting board 6 are within the field of view of the camera unit 120, or holds the measurement terminal 100 (external camera) in their hand and images the analysis device 1.

[0039] The display unit 130 has a display as a display device and, in addition to the screen display functions normally required in the measurement terminal 100, displays, for example, analysis results 154. The display unit 130 may also be configured as a touch panel. If the display unit 130 is configured as a touch panel, the user can perform operations on the measurement terminal 100, such as starting video recording, by operating the display unit 130. Since the display unit 130 can adopt various known configurations, a detailed explanation thereof is omitted.

[0040] The control unit 140 is composed of a processing unit and includes a device identification unit 142, a timing unit 144, and an analysis unit 146. The device identification unit 142 is configured to perform video capture processing to cause the camera unit 120 to capture video of the analysis device 1. The device identification unit 142 is also configured to recognize the marker 8 on the mounting sheet 6 from the video image data.

[0041] Furthermore, the device identification unit 142 is configured to determine whether or not it recognizes the markers 8 on the mounting sheet 6. If the device identification unit 142 does not recognize the markers 8 at the four corners of the analysis device 1, that is, if it determines that the markers 8 are not visible within the field of view of the video, it displays on the display unit 130 that the analysis device 1 is not correctly visible within the field of view of the video (that the analysis device 1 has not been properly imaged).

[0042] By having such a configuration, it is possible to prompt the user to adjust the imaging position, and to prevent variations in analysis results 154 depending on the user who performed the imaging.

[0043] The timing unit 144 is configured to perform a color change determination process that determines from the video image data whether or not the time measurement area 4 of the microfluidic device 2 has changed to a predetermined color, and a measurement start process that starts measuring time if it is determined that the time measurement area 4 has changed to a predetermined color.

[0044] The discoloration determination process includes a standard color information acquisition process to acquire color information of the standard color 7a for time measurement in the standard color section 7 of the base sheet 6, a discoloration information acquisition process to acquire color information of the time measurement area 4 of the microfluidic device 2, and a comparison process to compare the color information of the standard color 7a for time measurement with the color information of the time measurement area 4. In the comparison process, the timing unit 144 is configured to determine that the time measurement area 4 has changed to a predetermined color if the color difference between the color information of the standard color 7a for time measurement and the color information of the time measurement area 4 is within a predetermined range.

[0045] Specifically, in the comparison process, the timing unit 144 is configured to acquire color information of the standard color 7a for time measurement, calculate a color difference based on the color information, and determine whether the color difference is within a predetermined range. In this embodiment, the color information of the time measurement area 4 acquired by the timing unit 144 is the color information of the center of the outer area 4b of the time measurement area 4.

[0046] The control unit 140 is configured to perform selection information storage processing, which involves storing selection information 158 (selection information on which value to select from the color information for each component) in the storage unit 150, such as using the R value when analyzing the concentration of the first component.

[0047] As shown in Figure 5, the analysis unit 146 is configured to perform an analysis image acquisition process that causes the camera unit 120 to capture analysis image data 152 after a predetermined time has elapsed from the measurement start process. The analysis image acquisition process may perform still image capture (analysis image data 152) separately from the video capture process after the predetermined time has elapsed, or it may acquire image data at the time the predetermined time has elapsed as analysis image data 152 from the video of the video capture process.

[0048] Furthermore, the analysis unit 146 is configured to allow setting of multiple predetermined times. In this embodiment, multiple predetermined times are set, and the analysis unit 146 is configured to repeatedly perform the image acquisition process for analysis at each of the set predetermined times (for example, 15 seconds, 25 seconds, 35 seconds, 45 seconds, etc.). This reduces the burden on the user and improves the accuracy of the analysis result 154 when analyzing a microfluidic device 2 having multiple analysis regions 5 with different times required for the color reaction to progress sufficiently and become analyzable.

[0049] Furthermore, in the above-described configuration, the analysis unit 146 is configured to perform storage processing, as shown in Figure 8, to store images captured by the camera unit 120 of the colored liquid and the standard color (each outer region 51b to outer region 56b of the analysis area 5 and the standard color sections 7b and 7c for component analysis) as analysis image data (analysis image) 152 in the storage unit 150.

[0050] Furthermore, the analysis unit 146 is configured to perform a calculation process to calculate the analysis result 154 of the analysis region 5 of the microfluidic device 2 from the analysis image data 152. The calculation process includes a standard color information acquisition process to acquire the color information of the component analysis standard color sections 7b and 7c of the standard color section 7 of the base sheet 6, a color development information acquisition process to acquire the color information of the analysis region 5, a calibration curve creation process to create a calibration curve CC from the acquired color information of the component analysis standard color sections 7b and 7c, and an analysis result calculation process to calculate the analysis result 154 from the acquired color information of the analysis region 5 and the calibration curve CC.

[0051] In the standard color information acquisition process, the analysis unit 146 analyzes the first component (PO4) contained in the liquid. 3―When calculating the analysis result 154 regarding the concentration of the second component (NO2) contained in the liquid, the analysis unit 146 is configured to select the R color configuration from the color information of the lower component analysis standard color section 7c stored as analysis image data 152, based on the selection information 158, and to acquire the R value, which is the value of that color configuration, for each concentration (the R value of the first standard color 71, the R value of the second standard color 72, the R value of the third standard color 73, and the R value of the fourth standard color 74, respectively). Furthermore, when the analysis unit 146 calculates the analysis result 154 regarding the concentration of the third component (NO3) contained in the liquid, it is configured to select a color configuration of B based on the selection information 158 from the color information of the lower component analysis standard color unit 7c stored as analysis image data 152, and to acquire the B value, which is the value of that color configuration, for each concentration (the B value of the first standard color 71, the B value of the second standard color 72, the B value of the third standard color 73, and the B value of the fourth standard color 74, respectively).

[0052] Furthermore, in the standard color information acquisition process, the analysis unit 146 analyzes the fourth component (H +When calculating the analysis result 154 regarding the concentration of the fifth component (Ca) contained in the liquid, the analysis unit 146 is configured to select the R color configuration from the color information of the upper component analysis standard color section 7b stored as analysis image data 152, based on the selection information 158, and to obtain the R value, which is the value of that color configuration, for each concentration.

[0053] In the color information acquisition process, when the analysis unit 146 calculates an analysis result 154 regarding the concentration of the first component contained in the liquid, it is configured to select the R color configuration from the color information of the center of the outer region 54c stored as analysis image data 152, based on the selection information 158, and to acquire the R value, which is the value of that color configuration. Furthermore, when the analysis unit 146 calculates an analysis result 154 regarding the concentration of the second component contained in the liquid, it is configured to select the G color configuration from the color information of the center of the outer region 53b stored as analysis image data 152, based on the selection information 158, and to acquire the G value, which is the value of that color configuration. Furthermore, when the analysis unit 146 calculates an analysis result 154 regarding the concentration of the third component contained in the liquid, it is configured to select the B color configuration from the color information of the center of the outer region 55c stored as analysis image data 152, based on the selection information 158, and to acquire the B value, which is the value of that color configuration.

[0054] Furthermore, when calculating an analysis result 154 regarding the concentration of the fourth component contained in the liquid, the analysis unit 146 is configured to select a color configuration for R based on the selection information 158 from the color information of the center of the outer region 51b stored as analysis image data 152, and to obtain the R value, which is the value of that color configuration. Furthermore, when calculating an analysis result 154 regarding the concentration of the fifth component contained in the liquid, the analysis unit 146 is configured to select a color configuration for calculating the Hue value based on the selection information 158 from the color information of the center of the outer region 52b stored as analysis image data 152, and to obtain the value of that color configuration. Furthermore, when calculating an analysis result 154 regarding the concentration of the sixth component contained in the liquid, the analysis unit 146 is configured to select a color configuration for B based on the selection information 158 from the color information of the center of the outer region 56b stored as analysis image data 152, and to obtain the B value, which is the value of that color configuration.

[0055] In the calibration curve preparation process, as shown in Figure 9, the analysis unit 146 is configured to prepare a calculation calibration curve CC for the component for which the analysis result 154 is calculated, based on the values ​​obtained for each concentration from the color information of the component analysis standard color units 7b and 7c. This calculation calibration curve CC, for example, has the vertical axis as the values ​​related to color information such as the R value and the horizontal axis as PO4 3― This refers to the concentration of each component, such as the concentration of each component.

[0056] The analysis unit 146, in the case of analyzing the concentration of the first component, is capable of performing a process to create a linear calibration curve CC by plotting the R values ​​obtained from the four standard colors (first standard color 71, second standard color 72, third standard color 73, and fourth standard color 74) of the lower component analysis standard color unit 7c for each concentration (shown as plots 71r to 74r in Figure 9) during the standard color information acquisition process. Similarly, the analysis unit 146 is configured to perform the following processes for creating a calibration curve, which is a linear equation: if analyzing the concentration of the second component, plot the G values ​​obtained from the lower component analysis standard color section 7c for each concentration; if analyzing the concentration of the third component, plot the B values ​​obtained from the lower component analysis standard color section 7c for each concentration; if analyzing the concentration of the fourth component, plot the R values ​​obtained from the upper component analysis standard color section 7b for each concentration; if analyzing the concentration of the fifth component, plot the Hue values ​​obtained from the upper component analysis standard color section 7b for each concentration; and if analyzing the concentration of the sixth component, plot the B values ​​obtained from the upper component analysis standard color section 7b for each concentration.

[0057] In the analysis result calculation process, if the analysis is for the concentration of the first component, the analysis unit 146 substitutes the R value obtained from the color information of the center of the outer region 54c into the linear equation of the calibration curve CC, thereby calculating the first component (PO4 3― The analysis unit 146 is configured to perform a process to calculate analysis results 154 regarding the concentrations of the second to sixth components by substituting the following into the linear equations of the calibration curves created in the calibration curve creation process: G value obtained from the color information of the center of the outer region 53b for the analysis of the second component's concentration, B value obtained from the color information of the center of the outer region 55c for the analysis of the third component's concentration, R value obtained from the color information of the center of the outer region 51b for the analysis of the fourth component's concentration, Hue value obtained from the color information of the center of the outer region 52b for the analysis of the fifth component's concentration, and B value obtained from the color information of the center of the outer region 56b for the analysis of the sixth component's concentration.

[0058] In this embodiment, the analysis unit 146 calculates the analysis result 154 from the analysis image data 152 each time it acquires the analysis image data 152, but is not limited to this. The analysis unit 146 may also calculate the analysis result 154 from each of the analysis image data 152 all at once after it has acquired multiple sets of analysis image data 152.

[0059] Furthermore, as shown in Figure 8, the analysis unit 146 is configured to perform an analysis result display process that displays the calculated analysis results 154 on the display unit 130. In this embodiment, the analysis unit 146 is configured to display the analysis results 154 of multiple analysis areas 5 in a list on the display unit 130. For example, the analysis unit 146 displays the analysis results of NO2-N, P, and NO3-N of the analysis device 1 in a list on the display unit 130. In addition, the analysis unit 146 stores the calculated analysis results 154 in the storage unit 150.

[0060] The memory unit 150 is composed of a storage device and stores various data in a read-write manner. As shown in Figure 3, the memory unit 150 stores analysis image data 152, analysis results 154, analysis program 156, and selection information 158.

[0061] The analysis program 156 is an analysis program that causes the measurement terminal 100 to perform an analysis of the liquid to be analyzed that has been dropped onto the microfluidic device 2, and causes the measurement terminal 100 to perform a video capture process that causes the camera unit 120 of the measurement terminal 100 to capture a video of the microfluidic device 2, a color change determination process that determines from the video image data whether or not the time measurement area 4 has changed to a predetermined color, a measurement start process that starts measuring time if it is determined that the time measurement area 4 has changed to a predetermined color, an analysis image capture process that causes the camera unit 120 to capture analysis image data 152 after a predetermined time has elapsed since the measurement start process, and a calculation process that calculates the analysis result 154 of the analysis area 5 from the analysis image data 152.

[0062] Next, we will explain the methods for analyzing liquids. Broadly speaking, these methods include a preparation step for creating standard colors and an analysis step for analyzing the components contained in the liquid.

[0063] As shown in Figure 4, in the preparation step, first, images are taken of the first component colored at two or more densities to obtain multiple first component images (S10), and after S15, the value of one of the RGB color components (first value) is obtained for each density from the multiple first component images (S20).

[0064] Specifically, as shown in Figure 7, in S10, four standard solutions containing the first component at different concentrations (e.g., 0 ppm, 10 ppm, 30 ppm, and 50 ppm) are dropped onto the introduction sections 3 of the four microfluidic devices 2, respectively, causing each outer region 54c to change color. After a predetermined time (e.g., 60 seconds) has elapsed since dropping the standard solutions, the microfluidic devices 2 with colored outer regions 54c are imaged by the measurement terminal 100, and multiple first component images 21a to 21d are acquired.

[0065] Subsequently, in S15, the R, G, and B values ​​(collectively referred to as RGB values) of the RGB color model are obtained from multiple first-component images 21a to 21d. Furthermore, other color-related values ​​are calculated from the obtained RGB values. These other values ​​include, for example, Hue, Saturation, Value, Y, Pb (blue difference), and Pr (red difference). Note that RGB values ​​and other color-related values ​​are sometimes referred to as color information.

[0066] Then, based on this color information, calibration curves (calibration curves for standard color creation) are created for each value relative to concentration (ppm), such as calibration curves for R value, G value, B value, Hue value, Saturation value, etc. Next, by comparing the created calibration curves, a selection is made to determine which color component (R, G, B) value (R value, G value, B value) from the RGB values ​​will be used as the value for analyzing the first component (first value). There are no particular limitations to this selection method, but for example, the color component value of the calibration curve with the best linearity (for example, the one whose coefficient of determination is closest to 1 when expressed as a linear equation) may be adopted from among the multiple calibration curves created, or the color component value of the calibration curve with the largest slope may be adopted, or a color component value similar to the color that was produced may be adopted. If there is no suitable calibration curve for RGB values, the calibration curves for other color-related values ​​may be compared and the first value may be selected. In this embodiment, by performing the selection method described above, the calibration curve 24 of the R value shown in Figure 7 was the most preferable for the first component compared to other calibration curves (calibration curves for creating standard colors), and therefore, it was selected to use the R value from the RGB values ​​as the first component. Then, the selection information 158 of using the R value as the first component (selection information on which value from the color information to select for each component) is stored in the storage unit 150 by the control unit 140 of the measurement terminal 100 (see Figure 3).

[0067] Next, in S20, as shown in Table 27 of Figure 7, the first values ​​are obtained for each concentration from the calibration curve 24 created based on the above-mentioned multiple first component images 21a to 21d. Specifically, by substituting the values ​​of multiple concentrations (e.g., 0 ppm, 10 ppm, 30 ppm, 50 ppm) into the linear equation of the calibration curve 24, the R values ​​for each concentration (210, 185, 170, 140) are calculated as the first values ​​(these first values ​​are shown as "calculated R values" in Table 27). At this time, concentration numbers 1 to 4 are associated with the concentrations in descending order. Alternatively, the concentration numbers 1 to 4 could be associated with the R values ​​in descending order.

[0068] Next, as shown in Figures 4 and 7, images are taken of the second component colored at two or more densities to obtain multiple second component images 22a to 22d (S30). After S35, from the multiple second component images 22a to 22d, the value of one color configuration (second value) that is different from the color configuration of the first component among the RGB values ​​is obtained for each density (S40). These steps are performed by repeating the same procedure as in S10 to S20 for the second component.

[0069] Specifically, in S30, four standard solutions containing the second component at different concentrations (e.g., 0 ppm, 5 ppm, 12 ppm, 20 ppm) are dropped onto the introduction sections 3 of four microfluidic devices 2, respectively, to colorize each outer region 53b, thereby acquiring multiple second component images 22a to 22d. In S35, it is assumed that the calibration curve 25 for the G value was the most preferable for the second component compared to other calibration curves, and therefore the G value among the RGB values ​​was selected as the second component. In this case, the calibration curve for the R value may be excluded from the comparison beforehand. The G value is the value of one of the color configurations (G, B) that is different from the color configuration (R) of the first component. Furthermore, the selection information 158 of using the G value as the second component is stored in the storage unit 150 by the control unit 140 of the measurement terminal 100. In S40, as shown in Table 28 of Figure 7, the G values ​​for each concentration (200, 180, 150, 115) are calculated as secondary values ​​by substituting the values ​​of multiple concentrations (e.g., 0 ppm, 5 ppm, 12 ppm, 20 ppm) into the linear equation of calibration curve 25 (these secondary values ​​are shown as "Calculated G Values" in Table 28). At this time, the concentrations are associated with numbers 1 to 4 in descending order of concentration.

[0070] Next, as shown in Figures 4 and 7, images are captured of the third component colored at two or more densities to obtain multiple third component images 23a to 23d (S50). After S55, values ​​(third values) with a color configuration different from the color configuration of the first and second components among the RGB values ​​are obtained for each density from the multiple third component images 23a to 23d (S60).

[0071] Specifically, in S50, four standard solutions containing the third component at different concentrations (e.g., 0 ppm, 30 ppm, 80 ppm, 100 ppm) are dropped onto the introduction sections 3 of four microfluidic devices 2, respectively, to colorize each outer region 55c, thereby acquiring multiple third component images 23a to 23d. In S55, it is assumed that the calibration curve 26 for the B value was the most preferred among the other calibration curves for the third component, and therefore the B value among the RGB values ​​was selected as the third component. In this case, the calibration curves for the R and G values ​​may be excluded from the comparison beforehand. The B value is a value of a color configuration (B) that is different from the color configuration (R, G) of the first and second components. Furthermore, the selection information 158 of using the B value as the third component is stored in the storage unit 150 by the control unit 140 of the measurement terminal 100. In S60, as shown in Table 29 of Figure 7, the B values ​​for each concentration (210, 190, 170, 160) are calculated as third values ​​by substituting the values ​​of multiple concentrations (e.g., 0 ppm, 30 ppm, 80 ppm, 100 ppm) into the linear equation of calibration curve 26 (these third values ​​are shown as "Calculated B Values" in Table 29). At this time, concentration numbers 1 to 4 are assigned to the concentrations in descending order.

[0072] Next, as shown in Figures 4 and 7, a standard color is created that includes the first value (calculated R value) for each concentration of the first component, the second value (calculated G value) for each concentration of the second component, and the third value (calculated B value) for each concentration of the third component (S70). Specifically, as shown in Tables 27-29 in Figure 7, the RGB values ​​of the first to third components with the same concentration number are combined to create the color of the combined RGB values ​​as a standard color, as shown in Table 30.

[0073] For example, by combining the calculated R value of the first component's density number 1, "210" (see Table 27 in Figure 7), the calculated G value of the second component's density number 1, "200" (see Table 28 in Figure 7), and the calculated B value of the third component's density number 1, "210" (see Table 29 in Figure 7), a color with RGB values ​​(R value, G value, B value) of (210, 200, 210) is produced as the first standard color 71, as shown in Table 30 in Figure 7. Similarly, by combining the calculated R value of the first component's density number 2, "185" (see Table 27 in Figure 7), the calculated G value of the second component's density number 2, "180" (see Table 28 in Figure 7), and the calculated B value of the third component's density number 2, "190" (see Table 30 in Figure 7), a color with RGB values ​​of (185, 180, 190) is produced as the second standard color 72. Furthermore, by combining the calculated R value of the first component's density number 3 ("170"), the calculated G value of the second component's density number 3 ("150"), and the calculated B value of the third component's density number 3 ("170"), a color with RGB values ​​of (170, 150, 170) is created as the third standard color 73. Similarly, by combining the calculated R value of the first component's density number 4 ("140"), the calculated G value of the second component's density number 4 ("115"), and the calculated B value of the third component's density number 4 ("160"), a color with RGB values ​​of (140, 115, 160) is created as the fourth standard color 74. Note that the colors of each standard color depicted in Figure 7 and other drawings are for convenience only and differ from the actual colors determined by the above RGB values.

[0074] Next, as shown in Figures 1 and 4, the prepared standard colors 71 to 74 are applied to a sheet-like material to create the standard color section 7 (lower component analysis standard color section 7c), thereby creating the base sheet 6 (S80). At this time, the same procedure is followed on the sheet-like material to create the upper component analysis standard color section 7b. Additionally, the time measurement standard color 7a, marker 8, and device placement area 9 are created on the sheet-like material. The base sheet 6 is manufactured through these steps. These steps can also be called the manufacturing method for the analysis base sheet (base sheet 6).

[0075] Furthermore, in the preparation process selection steps (S15, S35, S55), if other color-related values ​​such as Hue values ​​are selected as values ​​for analyzing the concentration of predetermined components, then the color composition values ​​(RGB values) necessary to calculate those values ​​should be obtained for each concentration to create standard colors.

[0076] Next, the analysis process will be explained, primarily using Figure 5. The analysis process generally involves dropping the liquid to be analyzed onto a microfluidic device 2, and then imaging the analysis device 1, which includes the microfluidic device 2 with the liquid dropped onto it, with a measurement terminal 100 to analyze the liquid. In this embodiment, the liquid to be analyzed contains at least one of the first and second components, and the concentration of each component is unknown. In other embodiments, the liquid to be analyzed may contain at least one of the first, second, and third components, and the concentration of each component may be unknown.

[0077] The analysis process can be broadly divided into the following steps: a video acquisition step in which the camera unit 120 captures a video of the microfluidic device 2 after a liquid has been dropped onto the microfluidic device 2; a color change determination step in which the control unit 140 determines from the video image data whether the time measurement area 4 has changed to a predetermined color; a measurement start step in which the control unit 140 starts measuring time if it is determined that the time measurement area 4 has changed to a predetermined color; an analysis image acquisition step in which the camera unit 120 captures analysis image data 152 after a predetermined time has elapsed since the measurement start step; and a calculation step in which the control unit 140 calculates the analysis result 154 of the analysis area 5 from the analysis image data 152. Figure 8 schematically shows the procedures performed on the microfluidic device 2 and the measurement terminal 100, etc., during the analysis process.

[0078] The analysis process will be described in detail below. In this embodiment, the microfluidic device 2 will be described as being in a state where it has been placed in advance on the device placement area 9 of the mounting sheet 6.

[0079] First, the user points the camera unit 120 of the measurement terminal 100 towards the analysis device 1 and operates the measurement terminal 100 to select the start button to begin the analysis of the microfluidic device 2, thereby starting the analysis process. If the display unit 130 is configured as a touch panel, the selection of the start button is done by tapping, for example.

[0080] After the measurement terminal 100 receives the selection operation of the start button, the device identification unit 142 of the control unit 140 of the measurement terminal 100 causes the camera unit 120 of the measurement terminal 100 to start imaging (S100). The camera unit 120 captures a video of the analysis device 1 (S110: video capture process). The device identification unit 142 also determines whether or not it has recognized the marker 8 on the mounting sheet 6 of the analysis device 1 from the video image data (S120).

[0081] If the device identification unit 142 of the control unit 140 of the measurement terminal 100 does not recognize the four markers 8 on the mounting sheet 6 of the analysis device 1 (NO in S120), the display unit 130 of the measurement terminal 100 displays a message indicating that the analysis device 1 is not properly captured within the video's field of view, prompting the user to adjust the imaging position.

[0082] If the device identification unit 142 of the control unit 140 of the measurement terminal 100 recognizes the four markers 8 of the analysis device 1 (YES in S120), the video imaging process continues. In the video imaging process, if the device identification unit 142 correctly recognizes the markers 8, the camera unit 120 of the measurement terminal 100 captures the area within the markers of the analysis device 1, and therefore the camera unit 120 images the standard color area 7 of the mounting sheet 6 together with the microfluidic device 2.

[0083] After the video capture process begins, the user drops the liquid to be analyzed (test solution) into the introduction section 3 of the microfluidic device 2 of the analysis device 1 (see Figure 8). Subsequently, the timing unit 144 of the control unit 140 of the measurement terminal 100 determines from the video image data whether the time measurement area 4 has turned a predetermined color (S130-S150: color change determination process). Specifically, the timing unit 144 acquires the color information of the standard time measurement color 7a of the base paper 6 from the image data (S130). The timing unit 144 also acquires the color information of the outer area (timing color area) 4b of the time measurement area 4 of the microfluidic device 2 (S140).

[0084] Then, the timing unit 144 of the control unit 140 of the measurement terminal 100 compares the color information of the standard color 7a for time measurement of the acquired standard color section 7 of the base sheet 6 with the color information of the time measurement area 4 of the microfluidic device 2 and determines whether the color difference is within a predetermined error range (S150). If the color difference between the color information of the standard color 7a for time measurement and the color information of the time measurement area 4 is within a predetermined range (YES in S150), the timing unit 144 determines that the time measurement area 4 has turned to a predetermined color and starts measuring time (S160: measurement start step).

[0085] On the other hand, if the color difference between the color information of the standard color 7a for time measurement and the color information of the time measurement area 4 is not within a predetermined range (NO in S150), the timing unit 144 of the control unit 140 of the measurement terminal 100 acquires and compares the color information of the standard color 7a for time measurement and the color information of the time measurement area 4 again.

[0086] Subsequently, the analysis unit 146 of the control unit 140 of the measurement terminal 100 waits until the analysis area 5 of the microfluidic device 2 of the analysis device 1 shows a color reaction, and determines whether a predetermined time has elapsed (S170). If it is determined that the predetermined time has not elapsed (NO in S170), the analysis unit 146 waits again.

[0087] If it is determined that a predetermined time has elapsed (YES in S170), the analysis unit 146 of the control unit 140 of the measurement terminal 100 instructs the camera unit 120 to capture analytical image data 152. The camera unit 120 captures analytical image data 152 in which the standard color area 7 is shown together with the microfluidic device 2 in its positioned state (S180: analytical image capture process). The analysis unit 146 then performs a calculation process to calculate the analysis result 154 of the analysis area 5 from the analytical image data 152 (S190: calculation process).

[0088] The calculation process according to this embodiment will be described in detail below with reference to Figure 6. Specifically, the analysis unit 146 performs the above-mentioned standard color information acquisition process to acquire the color information of the standard color sections 7b and 7c for component analysis of the standard color section 7 of the base sheet 6 (S192: standard color information acquisition process). For example, when the analysis unit 146 analyzes the density of the first component, it acquires the R value of the RGB values ​​from the standard colors 71 to 74 for each density based on the selection information 158. When the analysis unit 146 analyzes the density of the second component, it acquires the G value of the RGB values ​​from the standard colors 71 to 74 for each density based on the selection information 158. When the analysis unit 146 analyzes the density of the third component, it acquires the B value of the RGB values ​​from the standard colors 71 to 74 for each density based on the selection information 158.

[0089] Next, the analysis unit 146 performs the above-mentioned color information acquisition process to acquire color information of the analysis region 5 of the microfluidic device 2 from the analysis image data 152 (S194: color information acquisition process). For example, when the analysis unit 146 analyzes the concentration of the first component, it acquires the R value of the RGB values ​​from the outer region 54c based on the selection information 158; when the analysis unit 146 analyzes the concentration of the second component, it acquires the G value of the RGB values ​​from the outer region 53b based on the selection information 158; and when the analysis unit 146 analyzes the concentration of the third component, it acquires the B value of the RGB values ​​from the outer region 55c based on the selection information 158.

[0090] Next, the analysis unit 146 performs the calibration curve preparation process described above to create calibration curves CC (see Figure 9) for each component from the acquired color information of the standard color units 7b and 7c for component analysis (S196: Calibration curve preparation process). Next, the analysis unit 146 performs the analysis result calculation process described above to calculate the analysis result 154 from the acquired color information of the analysis region 5 and the calibration curve CC (S198: Analysis result calculation process).

[0091] Subsequently, the analysis unit 146 of the control unit 140 of the measurement terminal 100 determines whether or not the calculation of each component of the liquid has been completed (S200). If it is determined that the calculation has not been completed (NO in S200), the analysis unit 146 again determines whether or not a predetermined time has elapsed (S170), and repeats the subsequent steps (S180 to S200).

[0092] Furthermore, when the analysis unit 146 of the control unit 140 of the measurement terminal 100 determines that all calculations have been completed (YES in S200), it displays the calculated analysis results 154 on the display unit 130 of the measurement terminal 100, as shown in Figure 8 (S210: Analysis result display step). Through these steps, a series of analysis steps of the analysis device 1 by the measurement terminal 100 according to this embodiment are performed.

[0093] Next, the effects of this embodiment will be explained. The analytical method of this embodiment involves the first component (PO4 3-A method for analyzing a liquid containing at least one of the first component (NO2), wherein the first component is colored at two or more concentrations, and from a plurality of first component images 21a to 21d obtained by imaging a microfluidic device 2, the value of one of the RGB color components (R) (R value) is obtained for each concentration, and from a plurality of second component images 22a to 22d obtained by imaging a microfluidic device 2, colored at two or more concentrations, the value of one of the RGB color components (G) that is different from the color component of the first component (G value) is obtained for each concentration, and at least for each concentration of the first component The method includes a preparation step for creating standard colors 71-74 that include the color composition values ​​of the first component and the color composition values ​​for each concentration of the second component, and an analysis step for analyzing the first or second component using analytical image data 152 obtained by imaging the microfluidic device 2 in which the liquid has been colored and the standard colors 71-74, by (1) using the value of the color composition (R) of the first component (R value) from the RGB values ​​when analyzing the first component, and (2) using the value of the color composition (G) of the second component (G value) from the RGB values ​​when analyzing the second component.

[0094] With this configuration, the standard colors used in the analysis process can be shared when analyzing the two components (first component and second component). This reduces the number of standard colors in the liquid (microfluidic device 2 in this embodiment) compared to when two sets of standard colors (two color charts) are created for the two components. Furthermore, the space on the base sheet 2 can be increased, allowing for an increase in the number of components that can be analyzed by a single microfluidic device 2.

[0095] Furthermore, in the preparation step, a calibration curve is created using RGB values ​​from multiple first-component images 21a to 21d or multiple second-component images 22a to 22d, and the values ​​of which color configuration from the RGB values ​​to use are selected.

[0096] With this configuration, standard colors 71-74 can be created using calibration curves 24 and 25 for the selected color composition values. This makes it possible to create standard colors 71-74 that allow for accurate analysis of the concentration of each component.

[0097] Furthermore, in the analysis process, an image for analysis is obtained by imaging with a liquid that has been colored placed near the standard colors 71 to 74. Also in the analysis process, in a microfluidic device 2 comprising an introduction section 3 for dropping liquid and an outer region 54c connected to the introduction section 3 where the first component is colored, an image for analysis 152 is obtained by dropping liquid into the introduction section 3 and imaging the outer region 54c and the standard colors 71 to 74.

[0098] With this configuration, analysis image data 152 can be acquired under the same imaging environment (brightness, color temperature, etc.), and the color information of the outer region 54c and standard colors 71-74 can be compared. This makes the analysis results less susceptible to the imaging environment and improves the accuracy of the analysis results 154.

[0099] Furthermore, in the analysis process, a liquid is dropped onto a microfluidic device 2 having an outer region 54c where the first component exhibits coloration and an outer region 53b where the second component exhibits coloration, and then analyzed. With this configuration, it is possible to provide an analytical method that can suitably analyze multiple components.

[0100] Furthermore, in the analysis process, in a microfluidic device 2 equipped with an outer region (time-sensitive color-changing region) 4b connected to the introduction region 3, liquid is dropped into the introduction region 3, and after the outer region 4b changes color, the outer region 54c and standard colors 71-74 are imaged to acquire analytical image data 152.

[0101] With this configuration, it becomes unnecessary for the operator to measure the time required for sufficient color development in the outer region 54c, thus reducing the burden on the user and suppressing measurement errors.

[0102] Furthermore, in the preparation process, from multiple third-component images obtained by capturing the third component (NO3) at concentrations of 2 or more, the value of the color configuration (B) among the RGB values, which is different from the color configuration of the first and second components (B value), is obtained for each concentration, and standard colors 71 to 74 are created that include the color configuration values ​​for each concentration of the third component.

[0103] With this configuration, the standard colors used in the analysis process can be shared when analyzing each of the three components (first component, second component, and third component). This reduces the number of standard colors required for the colored liquid (microfluidic device 2 in this embodiment) compared to when three sets of standard colors (three color charts) are created for the three components.

[0104] Furthermore, the analytical device 1 of this embodiment is for the first component (PO4 3- The device 1 analyzes a liquid containing at least one of the first component (NO2) and a second component (NO2), comprising: an outer region 54c in which the first component exhibits color; an outer region 53b in which the second component exhibits color; and standard color sections 7b and 7c for component analysis, which are colored with standard colors 71 to 74. The standard colors 71 to 74 are colored such that they include at least the values ​​(R values) for each concentration of any one color configuration (R) among the RGB values ​​obtained from a plurality of first component images 21a to 21d obtained by imaging the first component at two or more concentrations, and the values ​​(G values) for each concentration of any one color configuration (G) different from the color configuration of the first component among the RGB values ​​obtained from a plurality of second component images 22a to 22d obtained by imaging the second component at two or more concentrations.

[0105] Furthermore, the analysis system 110 of this embodiment comprises the analysis device 1, a camera unit (imaging unit) 120 that images the liquid colored by the analysis device 1 and the standard colors 71 to 74, and a control unit 140 that analyzes the first or second component of the analysis image data 152 captured by the camera unit 120, using (1) the value of the color composition (R) of the first component among the RGB values ​​(R value) when analyzing the first component, and (2) the value of the color composition (G) of the second component among the RGB values ​​(G value) when analyzing the second component.

[0106] With this configuration, it becomes possible to provide an analytical device 1 and an analytical system 110 that can achieve the same effects as described above.

[0107] <Embodiment 2> Next, Embodiment 2 of the present invention will be described. In this embodiment, the same reference numerals are used for the same parts as in the above embodiment, and redundant explanations of the structure, operation, and effect will be omitted.

[0108] As shown in Figure 10, the analysis device 201 in this embodiment comprises a microfluidic device 202 and a base plate 206. The microfluidic device 202 comprises a first analysis region group 11 and a second analysis region group 12. The first analysis region group 11 comprises an introduction section 203a and a plurality of channels 204, 250~256 connected to the introduction section 203a and extending radially from the outer edge of the introduction section 203a. Each channel 204, 251~257 includes an analysis region 251~257 and a time measurement region 204. The second analysis region group 12 comprises an introduction section 203b and a plurality of channels connected to the introduction section 203b and extending radially from the outer edge of the introduction section 203b, comprising analysis regions 261~265.

[0109] Analytical regions 251-257 include the first analytical region 251 for analyzing the first component, the second analytical region 252 for analyzing the second component, the third analytical region 253 for analyzing the third component, the fourth analytical region 254 for analyzing the fourth component, the fifth analytical region 255 for analyzing the fifth component, the sixth analytical region 256 for analyzing the sixth component, and the seventh analytical region 257 for analyzing the seventh component. Analytical regions 261-265 include the eighth analytical region 261 for analyzing the eighth component, the ninth analytical region 262 for analyzing the ninth component, the tenth analytical region 263 for analyzing the tenth component, the eleventh analytical region 264 for analyzing the eleventh component, and the twelfth analytical region 265 for analyzing the twelfth component. These first to twelfth components are considered to be different components from each other, for example, pH, Ca, NO2, PO4 3- Each component is selected from among the analyte components such as NO3 and K.

[0110] The standard color section 207 on the base sheet 206 includes a standard color 7a for time measurement and standard color sections 207b1, 207b2, 207c1, and 207c2 for component analysis. The lower left standard color section 207c1 for component analysis is composed of multiple (four in this embodiment) standard colors 71 to 74 that are different in color from each other. The other standard color sections 207b1, 207b2, and 207c2 for component analysis have the same configuration as the lower left standard color section 207c1, and their description is omitted.

[0111] For example, the lower left component analysis standard color section 207c1 is used to analyze the concentration of the first component that exhibits color in the first analysis region 251, the concentration of the second component that exhibits color in the second analysis region 252, and the concentration of the third component that exhibits color in the third analysis region 253. The upper left component analysis standard color section 207b1 is used to analyze the concentration of the fourth component that exhibits color in the fourth analysis region 254, the concentration of the fifth component that exhibits color in the fifth analysis region 255, and the concentration of the sixth component that exhibits color in the sixth analysis region 256. The upper right component analysis standard color section 207b2 is used to analyze the concentration of the seventh component that exhibits color in the seventh analysis region 257, the concentration of the eighth component that exhibits color in the eighth analysis region 261, and the concentration of the ninth component that exhibits color in the ninth analysis region 262. The lower right standard color section 207c2 for component analysis is used to analyze the concentration of the 10th component that exhibited color in the 10th analysis region 263, the concentration of the 11th component that exhibited color in the 11th analysis region 264, and the concentration of the 12th component that exhibited color in the 12th analysis region 265.

[0112] <Embodiment 3> Next, Embodiment 3 of the present invention will be described. In this embodiment, the same reference numerals are used for the same parts as in the above embodiment, and redundant explanations of the structure, operation, and effect will be omitted.

[0113] As shown in Figure 11, the analysis device 301 in this embodiment comprises a test strip 302 and a backing sheet 306. The test strip 302 comprises a first analysis area 351 for analyzing a first component and a second analysis area 352 for analyzing a second component.

[0114] In the standard color section 307 of the base sheet 306, the component analysis standard color section 307c is composed of multiple (four in this embodiment) standard colors 71 to 74 that are different in color from each other. The component analysis standard color section 307c is used to analyze the concentration of the first component that exhibits color in the first analysis area 351 and the concentration of the second component that exhibits color in the second analysis area 352.

[0115] <Other Embodiments> The present invention is not limited to the embodiments described above and in the drawings. For example, the following embodiments are also included within the technical scope of the present invention, and furthermore, various modifications can be made without departing from the spirit of the invention.

[0116] In addition to the embodiments described above, the color information included in the standard color (the color configuration for creating the standard color) can be modified as appropriate. For example, the standard color may be created by combining the R value of the first component, the G value of the second component, and an arbitrary B value (for example, a value of 0 or 255 in the RGB color model).

[0117] In addition to the embodiments described above, the steps in the preparation process up to the creation of the standard color can be modified as appropriate. For example, in Embodiment 1 described above, the steps of acquiring component images of each component (see S10, S30, S50 in Figure 4) may be performed, followed by the step of selecting a color configuration for each component (see S15, S35, S55 in Figure 4), and then the step of acquiring each value from the calibration curve of the selected color configuration (see S20, S40, S60 in Figure 4). [Explanation of Symbols]

[0118] 1, 201, 301…Analytical device, 2, 202…Microfluid device, 3, 203a, 203b…Introduction section, 4b…Outer area (timing color section), 7b, 7c, 207b1, 207b2, 207c1, 207c2, 307c…Standard color section for component analysis (standard color section), 21a~21d…First component image, 22a~22d…Second component image, 23a~23d…Third component image, 24…Measurement line, 25…Measurement line, 26…Measurement line, 53b…Outer area (second coloring section), 54c…Outer area (first coloring section), 55c…Outer area (third coloring section), 71~74…Standard color, 110…Analytical system, 120…Camera section, 140…Control section, 152…Analytical image data

Claims

1. A method for analyzing a liquid containing at least one of the first and second components, From multiple first component images obtained by capturing the first component at two or more different concentrations, the value of one of the RGB color components is obtained for each concentration. From a plurality of second-component images obtained by capturing the second component at two or more concentrations, the RGB values ​​of one color configuration different from the color configuration of the first component are obtained for each concentration. A preparation step for creating a standard color that includes at least the color composition values ​​for each concentration of the first component and the color composition values ​​for each concentration of the second component, An analytical method comprising: an analytical step of analyzing an analytical image obtained by capturing the liquid after it has been colored and the standard color, wherein (1) when analyzing the first component, the value of the color composition of the first component among the RGB values ​​is used to analyze the first component or the second component; and (2) when analyzing the second component, the value of the color composition of the second component among the RGB values ​​is used to analyze the first component or the second component.

2. The analysis method according to claim 1, wherein in the preparation step, a calibration curve is prepared using RGB values ​​from the plurality of first component images or the plurality of second component images, and the values ​​of which color configuration among the RGB values ​​are to be used are selected.

3. The analysis method according to claim 1 or 2, wherein in the analysis step, an image is taken of the liquid that has exhibited color in the vicinity of the standard color to obtain the analysis image.

4. The analytical method according to claim 1 or 2, wherein the analytical step involves dropping the liquid onto a microfluidic device having a first color-exposing section where the first component exhibits color and a second color-exposing section where the second component exhibits color, and then performing the analysis.

5. The analysis method according to claim 1 or 2, wherein the analysis step is performed using a microfluidic device comprising an introduction section for dropping the liquid and a first coloring section connected to the introduction section where the first component exhibits color, the liquid is dropped into the introduction section, and the first coloring section and the standard color are imaged to obtain the analysis image.

6. The analysis method according to claim 5, wherein in the analysis step, the microfluidic device having a time-timing color-changing section connected to the introduction section, the liquid is dropped into the introduction section, and after the time-timing color-changing section changes color, the first color-changing section and the standard color are imaged to obtain the analysis image.

7. In the preparation step, from a plurality of third-component images obtained by capturing the third component at two or more concentrations, values ​​of RGB with a color configuration different from that of the first and second components are obtained for each concentration. The analytical method according to claim 1 or 2, comprising producing the standard color including the color composition values ​​for each concentration of the third component.

8. A liquid analysis device comprising at least one of the first and second components, The first color-exposing portion in which the first component exhibits color, The second color-exposing portion in which the second component exhibits color, It comprises a standard color section that is colored with a standard color, The analytical device is colored such that the standard color includes at least the values ​​for each color configuration of any one of the RGB values ​​obtained from a plurality of first component images obtained by imaging the first component at two or more concentrations, and the values ​​for each color configuration of any one of the RGB values ​​obtained from a plurality of second component images obtained by imaging the second component at two or more concentrations, which are different from the color configuration of the first component.

9. The analytical device according to claim 8, The aforementioned analytical device includes an imaging unit that images the colored liquid and the standard color, An analysis system comprising: a control unit that analyzes the first component or the second component of an analysis image captured by the imaging unit, using (1) the color composition value of the first component from among the RGB values ​​when analyzing the first component, and (2) the color composition value of the second component from among the RGB values ​​when analyzing the second component.

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