Plant pathogen resistance inducer and disease control method containing alkaline earth metal peroxides and iron or iron-containing inorganic compounds

Abstract

The present invention provides a method for controlling bacterial or fungal diseases of agricultural and horticultural plants such as vegetables or grains, which contributes to reducing the environmental burden on the field and is free from phytotoxicity. [Solution] A plant pathogen resistance inducer containing the following agents A and B, wherein the amount of each agent applied to one horticultural plant is as shown below: Agent A: Preparation containing calcium peroxide or magnesium peroxide: 1-100g / strain Agent B: Preparation containing iron or iron-containing inorganic compounds: 1-100g / strain A resistance inducer wherein agent A and agent B are active ingredients, and agent A contains 10 to 60% by mass of CaO2 or the like in agent A.

Description

[Technical Field]

[0001] The present invention relates to a plant pathogenic fungal resistance inducer comprising a composite material containing an alkaline earth metal peroxide such as calcium peroxide or magnesium peroxide and iron or an iron-containing inorganic compound, and a disease control method using the resistance inducer for soil mixing treatment in planting holes. [Background technology]

[0002] Bacterial wilt, a soilborne bacterial disease affecting crops such as tomatoes and eggplants, is a global problem, resulting in enormous economic losses of hundreds of billions of yen annually. The bacterial wilt fungus invades the roots of plants and multiplies within the xylem vessels. When infected, the fungus's proliferation within the xylem vessels inhibits the supply of water and other nutrients to the entire plant, causing the plant to wilt. Toxins released by the fungus cause necrosis of plant cells, ultimately leading to death. Because the pathogen can survive deep in the soil for extended periods and recurs when a suitable host plant is planted, bacterial wilt is a difficult disease to control once it has occurred in an area.

[0003] Conventional control techniques for bacterial wilt include soil fumigation with chloropicrin, the use of resistant varieties, and the use of antibiotics. However, all of these methods have many remaining problems in terms of control effectiveness, cost, and environmental impact. Against this backdrop, there is a need to develop control methods that are cheaper, have less environmental impact, and are more effective than conventional methods.

[0004] On the other hand, disease resistance inducers have recently attracted attention as one of the materials for environmentally friendly pest control. Disease resistance inducers are chemicals that exert disease control effects by increasing the plant's inherent resistance to disease and inducing disease tolerance. Since they do not directly kill pathogens, they have the advantage of having a low risk of developing resistant strains, which is a problem when using fungicides.

[0005] Examples of environmentally friendly agricultural materials include the conventional use of alkaline earth metal peroxides, such as calcium peroxide, as oxygen supply agents to improve rice seedling establishment or as growth promoters for vegetables (Patent Documents 1 and 2, etc.). Disease resistance inducers against bacterial wilt using peroxides such as calcium peroxide and plant-derived iron-phenolic hydroxyl group-containing composite materials are also being investigated (Patent Document 3). However, there is a need to develop a control method that is cheaper, has less environmental impact, and is highly effective in controlling the disease. [Prior art documents] [Patent Documents]

[0006] [Patent Document 1] Japanese Patent Application Publication No. 1-262713 [Patent Document 2] Japanese Patent Publication No. 2024-100716 [Patent Document 3] Patent application No. 2025-60164 [Patent Document 4] Japanese Patent Publication No. 2021-155303 [Overview of the Initiative] [Problems that the invention aims to solve]

[0007] The present invention relates to a method for controlling bacterial or fungal diseases of agricultural and horticultural plants such as vegetables or grains, using a formulation in which a composite material obtained by mixing an alkaline earth metal peroxide, such as calcium peroxide or magnesium peroxide, with iron or an iron-containing inorganic compound as an active ingredient. The objective is to find a simple, practical, and phytotoxic disease control method that contributes to reducing environmental burden and labor costs in the field. [Means for solving the problem]

[0008] The present invention aims to solve the aforementioned problems and consists of the following:

[0009] [1] Contains the agents represented by Agent A and Agent B below, The amount of each agent applied to one horticultural plant (or seedling) is shown below: Resistance-inducing agents against plant pathogens: Agent A: Preparations containing calcium peroxide or magnesium peroxide: 1g to 100g / pack Agent B: Iron or iron-containing inorganic compound: 1g to 100g / strain And, A resistance inducer in which agent A is the active ingredient, and which contains 10% by mass or more and 60% by mass or less of calcium peroxide or magnesium peroxide in agent A.

[0010] In the resistance inducer described in [2][1], the mixing ratio (mass ratio) of agent A and agent B is, A resistance-inducing agent where the ratio of agent A to agent B is 1:0.02 to 1:10.

[0011] In the resistance inducer described in [3][1], the mass of calcium peroxide or magnesium peroxide in agent A (M E The mass of iron in agent B relative to (M F ) Mass ratio M F / M E However, it is a resistance inducer with a value between 0.01 and 2.0.

[0012] A method for controlling bacterial or fungal diseases by mixing a resistance-inducing agent against plant pathogens, as described in any of [4][1] to [3], into the soil of the planting hole.

[0013] [5] The method for controlling bacterial diseases as described in [4], wherein the bacterial disease is tomato bacterial wilt.

[0014] [6] The method for controlling fungal diseases described in [4], wherein the fungal disease is sweet potato fusarium wilt. [Effects of the Invention]

[0015] The resistance inducer against plant pathogenic fungi of the present invention causes little phytotoxicity to agricultural and horticultural plants such as vegetables, and is useful as an agent for use in a method for controlling bacterial diseases or filamentous fungal diseases. In addition, the disease control method of the present invention contributes to reducing the environmental load in fields and work costs, and is useful as a simple, practical, and phytotoxicity-free disease control method. [Brief Description of the Drawings]

[0016] [Figure 1] This is a cross-sectional view of 1 "ridge" in 10 "soil (field)" using the "resistance inducer against plant pathogenic fungi" of the present invention, where 2 "planting holes" (the region indicated by the dashed square (2 inside a circle)) are dug, agent A and agent B are granulated and scattered into the 2 "planting holes", and 5 "seedlings" of the plant including 3 "nursery soil" (the region indicated by the dashed square (3 inside a circle)) are placed inside the 2 "planting holes" on the granule-scattered 1 "ridge". [Figure 2] This is a cross-sectional view of 1 "ridge" in which a mixture of 8 "agent A" and 9 "agent B" of the present invention and soil is granulated and scattered inside 2 "planting holes" (spread over the wall surface and bottom surface of the soil hole), and 5 "seedlings" of the plant including 3 "nursery soil" are arranged inside the 2 "planting holes". [Figure 3] This is a cross-sectional view of 1 "ridge" of the present invention, in which 8 "agent A" and 9 "agent B" are mixed into 3 "nursery soil", and the mixture is granulated and scattered into 2 "planting holes" of the same size as the 3 "nursery soil". [Figure 4] This is a cross-sectional view of 1 "ridge" in which planting holes are dug in 1 "ridge", 8 "agent A", 9 "agent B" and soil are mixed and placed at the bottom of the holes, and 5 "seedlings" and 3 "nursery soil" of the present invention are granulated and scattered on the top of the "8 agent A + 9 agent B" layer. [Figure 5] This is a cross-sectional view of 1 "ridge" of the present invention, in which 2 "planting holes" are dug in 1 "ridge", 5 "seedlings" and 3 "nursery soil" are placed in the 2 "planting holes", and 8 "agent A", 9 "agent B" and soil are mixed, granulated and scattered (covered) on the upper part of 3 "nursery soil" (the root base of the seedlings). [Figure 6]This diagram shows 50 seedlings (after growth) and 40 roots (after growth) that have grown beyond the 2 planting holes in the soil after the disease control method of the present invention has been applied. The schematic diagram shows that a large number of 99 bacteria (bacterial wilt bacteria) (granular with jagged edges) are present near the 40 roots in the soil, as an example of the cause of bacterial disease. [Figure 7] This is a simplified overhead (schematic) diagram showing an embodiment of the present invention, where 5 seedlings are planted in several 2 planting holes in 1 furrow in an actual 10 "field". [Modes for carrying out the invention]

[0017] The embodiments of the present invention will be described in detail below, but the present invention is not limited to the embodiments described below and can be implemented in various forms within the scope of its gist.

[0018] The plant pathogenic fungal resistance inducer of the present invention (hereinafter also simply referred to as a resistance inducer) is This is a plant pathogenic fungicide containing the agents represented by [Agent A] and [Agent B] below, with the application rate of each agent per horticultural plant (or seedling) being as shown below. Agent A: Preparations containing calcium peroxide (CaO2) or magnesium peroxide (MgO2) (preparations containing alkaline earth metal peroxides): 1g to 100g / pack Agent B: Iron or iron-containing inorganic compounds: 1g to 100g / strain

[0019] The "Agent A" contained in the "Resistance Inducing Agent against Plant Pathogenic Fungi" of the present invention is an active ingredient and is a formulation containing an alkaline earth metal peroxide, with calcium peroxide or magnesium peroxide being specific representative examples. Furthermore, formulations containing these alkaline earth metal peroxides are alkaline earth metal peroxides; The mixture contains at least one of the following: an alkaline earth metal hydroxide, an alkaline earth metal carbonate, or a mineral. The form of agent A may be powder, granules, or granular, and if it is granular or granular, the particle size is preferably 1 mm to 10 mm.

[0020] For example, a method for producing a preparation containing calcium peroxide (CaO2) is to use calcium hydroxide Calcium (Ca(OH)2) and hydrogen peroxide (H2O2) aqueous solution are mixed and reacted, The mixture may be prepared into a liquid state, dehydrated, and CaO2 prepared, after which calcium hydroxide and calcium carbonate may be added in appropriate amounts. Then, through processes such as granulation, drying, and pulverization, a formulation consisting of a mixture (composition) containing at least one of calcium peroxide, calcium hydroxide, and calcium carbonate can be obtained. Additives such as sulfate hydrates may be added during the manufacturing process. The CaO2 content in the CaO2-containing formulation is preferably 10% to 60% by mass of the total CaO2-containing formulation, and more preferably 15% to 50% by mass.

[0021] Products containing magnesium peroxide (MgO2) can be manufactured using the same method as for CaO2. For example, a formulation consisting of a mixture (composition) containing at least one of magnesium peroxide, magnesium hydroxide, and magnesium carbonate can be obtained by mixing and reacting magnesium hydroxide (Mg(OH)2) and an aqueous solution of hydrogen peroxide (H2O2) (Patent Document 4). Additives such as sulfate hydrates may be added during the manufacturing process.

[0022] The minerals (mineral substances) contained in these alkaline earth metal peroxide-containing preparations can include calcium carbonate, clays (dry clay, wet clay), diatomaceous earth, talc, silica, silica sand, calcium carbonate, calcium sulfate (calcined gypsum), slaked lime, olivine sand, vermiculite, shirasu attaparjanite, pyrophyllite, acid clay, activated clay, fly ash, etc., and can be used in combination of one or more types.

[0023] The resistance-inducing agent of the present invention contains "iron or an iron-containing inorganic compound (hereinafter also simply referred to as an iron compound)" as "agent B". Any iron or iron compound can be used as "iron or iron compound" in the present invention. For example, metallic iron; water-soluble iron compounds such as iron(III) chloride (FeCl3), iron(III) sulfate (Fe2(SO4)3), iron(II) sulfate (FeSO4), and iron(II) nitrate (Fe(NO3)2); water-insoluble iron compounds such as iron(III) oxide (Fe2O3), iron(III) nitrate (Fe(NO3)3), iron(III) hydroxide (Fe(OH)3, FeO(OH)), and iron(II) hydroxide (Fe(OH)2); hydrates of the above iron compounds; natural iron ores such as pyrite, marcasite, siderite, magnetite, and goethite; natural products such as iron-containing soil, heme iron, and seashells; and those dissolved in acid. Of these, trivalent iron compounds such as iron(III) chloride and iron(III) sulfate are preferred.

[0024] The resistance-inducing agents containing "Agent A" and "Agent B" of the present invention will be described in detail below. In addition, a method for controlling plant pathogenic fungi using the resistance-inducing agents of the present invention will also be described.

[0025] The resistance-inducing agent of the present invention is characterized by being applied to the soil of a field when planting seedlings of vegetables or grains such as rice, by mixing or separately placing "Agent A" and "Agent B" only inside the hole where the seedlings are planted. At this time, the amount of agent applied per plant (or seedling) is 1g to 100g per plant for Agent A, and 1g to 100g per plant for Agent B. The details of the present invention will be specifically explained below using the simplified diagram of the present invention in Figure 1.

[0026] Figures 1 to 5 show the initial state of the "disease control method" using the "resistance inducer" of the present invention, immediately after planting the seedlings in the field. As described in the "Brief Description of the Drawings" above, Figure 6 is a schematic diagram of the seedlings after a certain period of time has passed and they have grown. Figure 7 is a schematic diagram illustrating a specific example of the state immediately after planting multiple seedlings in a field after creating ridges, digging planting holes in them, and treating them with "Agent A" and "Agent B". Generally, the field is typical farm soil, such as a field where vegetables or grains are cultivated. As a method of cultivating crops such as tomatoes and sweet potatoes, a conventional and well-known cultivation method involves creating one ridge by mounding soil in a tilled and fertilized field, digging two planting holes, and planting five seedlings (including four roots and three seedling growing medium) grown in, for example, a 10cm diameter x 10cm depth pot into one ridge.

[0027] Figures 1 and 2 show a cross-sectional view of a furrow in a soil (field) using a plant pathogenic fungus resistance inducer, which is a feature of the present invention. Two planting holes are dug, predetermined amounts of agent A and agent B are scattered into the two planting holes, and the soil on the walls of the planting holes and each agent are uniformly mixed with the soil using a trowel or the like to prevent the planting holes from being filled. Seedlings of plants containing seedling soil are planted inside the two planting holes. In Figure 2, agent A, which is calcium peroxide or magnesium peroxide, is represented by a white circle symbol "○". Also in Figure 2, agent B, which is an iron-PP composite material, is represented by a diamond symbol "◆". Thus, the resistance-inducing agent of the present invention may be placed in the planting holes and then mixed with an appropriate amount of soil, or the resistance-inducing agent of the present invention and an appropriate amount of soil may be mixed in advance and placed in the planting holes, and then the seedlings may be planted so that the resistance-inducing agent of the present invention is positioned around the seedling growing medium (bottom and periphery).

[0028] The size of a furrow can be adjusted according to the size and condition of the crop and field. A suitable height can be selected, such as 10 cm or less, 20 cm or less, or 30 cm or less. Generally, mounds of soil are arranged in long, straight rows in the field, and one or two planting holes are made in each furrow before planting seedlings (see, for example, Figure 7). The shape of the furrow is rectangular or trapezoidal in cross-section. The purpose of creating furrows is to increase the surface area in contact with water and air on the sides, which makes water supply, drainage, and soil temperature retention and cooling more efficient.

[0029] The holes for planting the seedlings (2 planting holes) can be the same size as the usual seedling planting holes and are not particularly limited. Specifically, for example, in the case of tomato seedlings, planting holes of an appropriate size to fit a seedling pot of about 10 cm in diameter and about 9 cm deep are preferable. The size of the planting holes may be changed according to the size of the roots of the transplanted seedlings.

[0030] The five seedlings (including three seedling pots, four roots, six leaves, and seven stems) may be grown from vegetable or grain seeds, or they may be commercially available. It is preferable that they be grown in seedling pots or cell trays before transplanting.

[0031] Figure 3 shows a specific example of applying the resistance-inducing agent of the present invention. While the specific example in Figure 2 is a method in which agents A and B are not mixed into the seedling growing medium, as shown in Figure 3, resistance-inducing agents A and B can also be applied (scattered) to seedlings, etc., so that the agents are located in the vicinity of four roots, such as five seedlings. Therefore, the resistance-inducing agent of the present invention can be mixed into the seedling growing medium in advance during seedling cultivation, and at the time of transplanting, the seedlings, etc., can be transplanted into the cultivation soil, such as a field or planter, together with the seedling growing medium.

[0032] Figure 4 shows a specific example of applying the resistance-inducing agent of the present invention. In the method shown in Figure 4, when planting seedlings, the resistance-inducing agent of the present invention (8 "Agent A" and 9 "Agent B") is placed in the bottom of a planting hole made in the soil (field) when planting seedlings, and the seedlings can be planted so that the resistance-inducing agent is located in the vicinity of the roots (lower part) of the seedlings.

[0033] Figure 5 shows a specific example of applying the resistance-inducing agent of the present invention. The resistance-inducing agent of the present invention (8 "Agent A" and 9 "Agent B") may be scattered (granulated) around seedlings, etc. (near the ground level of the stems) after transplanting, or it may be mixed into the soil after scattering so as not to damage the plant body such as seedlings, or a mixture of the resistance-inducing agent of the present invention and an appropriate amount of soil may be scattered as granules around seedlings, etc. after transplanting.

[0034] In each of the above application methods, the two agents, "Agent A," which consists of a peroxide forming the resistance-inducing agent of the present invention, and "Agent B," which consists of an iron-PP composite material, may be mixed in appropriate amounts beforehand and used, or appropriate amounts of "Agent A" and "Agent B" may be thoroughly mixed during application. Alternatively, "Agent A" and "Agent B" may be applied as granular agents with appropriate size and disintegrability by granulation, and appropriate additives may be selected and used as needed for the granulation. The mixing ratio (mass ratio) of "Agent A" and "Agent B" is: The ratio of Agent A to Agent B may be 1:0.02 to 1:10. A ratio of 1:0.05 to 1:5 for agent A to agent B is preferable. A ratio of 1:0.05 to 1:2 for agent A to agent B is more preferable. A ratio of 1:0.05 to 1:1 for Agent A to Agent B is even more preferable. Furthermore, if the soil is mixed in beforehand before treating the planting holes, the ratio with the soil can be adjusted as appropriate. The specific application amounts of Agent A and Agent B per horticultural plant can be appropriately determined depending on the type of seedling, etc. For example, Agent A may be 60g or less, 50g or less, 40g or less, 30g or less, or 5g or more, 10g or more, or 20g or more. Agent B may be 30g or less, 20g or less, or 1g or more, or 2g or more.

[0035] Furthermore, in the resistance inducer of the present invention, the mass of calcium peroxide or magnesium peroxide in agent A (M E The mass of iron in agent B relative to (M F ) Mass ratio M F / M EThe amount is preferably 0.01 to 4.0, may be 0.02 or more, 0.05 or more, 0.10 or more, may be 3.00 or less, and preferably 2.00 or less. For example, if the mass of CaO2 or MgO2 in agent A is 8 g, the mass of iron in agent B is preferably 0.08 to 16 g.

[0036] Furthermore, the resistance-inducing agent A or B of the present invention may contain water before or after mixing or when mixing with soil, and the water content may be set as appropriate. The water used in the resistance-inducing agent of the present invention is not particularly limited and may be ordinary agricultural water, such as tap water or well water.

[0037] The resistance-inducing agent of the present invention can be used by diluting it with water to contain, specifically, iron or an iron-containing inorganic compound at a concentration of 10 to 1000 times when actually using "Agent B".

[0038] By treating and using the plant pathogen resistance inducer of the present invention in this manner, it is less expensive than conventional technologies such as those described in Patent Document 3, and the effect of treating the planting hole is achieved, significantly reducing the amount of chemicals applied to the soil or natural environment. As a concrete example of this effect, Figure 6 illustrates the 50 "seedlings (after growth)" and the 40 "roots (after growth)" that have grown beyond the 2 "planting holes" in the soil after the disease control method of the present invention has been applied. As the plant in Figure 6 grows, as shown in the 50 seedlings (above ground, after growth), even if the 40 "roots (after growth)" extend beyond the chemically treated area from the planting hole, the plant has already been conferred with resistance, so it is possible to prevent infection (invasion) of tomato bacterial wilt (90 bacteria (bacteria (bacterial wilt fungus)) that is present throughout the soil.

[0039] The disease control method using the "resistance-inducing agent against plant pathogens" of the present invention differs from whole-soil treatment in that it is applied locally, and therefore the agent does not have the opportunity to come into contact with bacterial wilt fungi present throughout the soil. Thus, it is based on a different concept from the methods that utilize the bactericidal effect through contact between pathogens and agents as described in Patent Document 3, Non-Patent Document 1, or 2. The present invention can obtain a very high control effect against bacterial or fungal diseases, and it has been found that the disease control method using the agent of the present invention induces resistance in plants to pathogens.

[0040] The resistance inducer of the present invention may be a mixture of a solid carrier or a liquid carrier, and may also contain water, and optionally, additives (auxiliaries) such as surfactants, dispersants, penetrating agents, spreading agents, thickeners, antifreeze agents, binders, anticaking agents, decomposition inhibitors, preservatives, antisettling agents, and defoaming agents.

[0041] The resistance-inducing agent of the present invention can take various formulation forms by adding the above-mentioned additives (auxiliaries). Specifically, the bactericide can be used in any dosage form, such as liquid, emulsion, wettable powder, suspension (aqueous suspension formulation), granular water-soluble powder, granular wettable powder, emulsifier, suspender emulsion, powder, granules, gel, etc. It can also be used enclosed in a water-soluble packaging, or as a wet powder coating.

[0042] The crops to which the resistance-inducing agent of the present invention is used are not particularly limited, but it can be used for treating grains, vegetables, and various other plants.

[0043] The present invention is highly effective against bacterial diseases and fungal diseases. Examples of bacterial diseases include solanaceous bacterial wilt, such as tomato bacterial wilt, and examples of fungal diseases include sweet potato fusarium wilt and tomato verticillium wilt.

[0044] The control effect of the resistance-inducing agent of the present invention is evaluated using the control value. The control value is an index that represents the degree of control effect of the treated area against disease damage caused by bacteria, filamentous fungi, etc., in the untreated area. Generally, a higher control value indicates a greater control effect. Generally, Control value = [100 - (severity of disease in treated area / severity of disease in untreated area) × 100] It is calculated using the following formula. A higher control value indicates a greater control effect. [Examples]

[0045] The present invention will be described in detail below with reference to examples, but the present invention is not limited to these examples.

[0046] [Formulation Example 1 to Formulation Example 5] For "Agent A," the following commercially available calcium peroxide preparation (manufactured by Hodogaya Chemical Co., Ltd.) was used. Product name: Calper Granules 16 ("Calper" is a registered trademark) (A mixture containing 16% by mass of calcium peroxide (CaO2), which is an effective oxygen-supplying component.)

[0047] For "Agent B," we prepared iron(III) chloride = FeCl3 (manufactured by Kanto Chemical Co., Ltd.) and iron(III) oxide (ferric oxide) = Fe2O3 (manufactured by Kanto Denka Finetech Co., Ltd., brand name: No. 4500).

[0048] Table 1 shows the formulations and mixing ratios of Agent A and Agent B in each prepared preparation. In Table 1, the number in parentheses in the column for Agent A (g) represents the mass (g) of calcium peroxide contained, and the number in parentheses in the column for Agent B (g) represents the mass (g) of iron contained.

[0049] [Table 1]

[0050] [Example 1] <Tomato bacterial wilt control trial> Prepare the required number of containers (57 cm × 39 cm × 25 cm depth), and contaminated soil for tomato bacterial wilt (the bacterial suspension obtained by shake-culturing the bacterial wilt pathogen in SMB medium and harvesting the cells is mixed into the soil to adjust the density of the bacterial wilt pathogen in the soil to 10 3 cfu / g of soil, which was used as the test soil) was filled. The test plots were set as 4 plants (25 cm between plants) per container, with 6 replicates. On June 12, 2024, digging planting holes large enough to fit 9 cm pots in the container (4 locations per container) with a transplanting trowel, the Agent A and Agent B in the ratio described in Formulation Example 1 were granulated and scattered into the planting holes immediately before planting, and gently mixed by hand together with the soil in the planting holes. After the treatment, tomato seedlings (Momotaro 8: own-root cultivation) were planted. From June 12 (77 days after planting) to August 28 (the end date of the survey), the diseased plant rate of bacterial wilt and the disease occurrence by index were investigated for all plants in each plot every week. The disease severity and control value were calculated according to the following formulas (Equations 1 and 2). Phytotoxicity was evaluated according to the following criteria. The results are shown in Table 2, together with Comparative Example 1 (untreated plot) in which no drug was used.

[0051] Disease severity = [Σ(Number of plants by disease severity × Index) / (Number of investigated plants × 4)] × 100 —— (Equation 1) Index 0: Healthy 1: Wilting of the shoot apex is observed 2: Wilting of leaves is observed 3: The whole plant shows bacterial wilt symptoms 4: Dead plant Control value = [1 - (Disease severity of treated plot) / (Disease severity of untreated plot)] × 100 —— (Equation 2)

[0052] Phytotoxicity: Phytotoxicity was visually investigated at any time for the whole plant throughout the test period. -: No phytotoxicity observed +: Slight phytotoxicity symptoms observed ++: Moderate phytotoxicity symptoms observed +++: Severe phytotoxicity symptoms observed

[0053]

Table 2

[0054] [Example 2] We prepared a field contaminated with tomato bacterial wilt and created the necessary number of ridges (ridge width 1m). Each test plot consisted of 12 plants (40 cm apart) and was arranged in a 4-row configuration. On September 18, 2024, each furrow was divided into 1m x 3m sections, and planting holes large enough to accommodate 9cm pots were dug on the surface of each furrow (12 locations / section) using a trowel. Agents A and B, in the proportions described in Formulation Examples 1 to 3, were scattered into the planting holes immediately before transplanting and lightly mixed by hand with the soil in the planting holes. Tomato seedlings (Momotaro 8: self-rooted cultivation) were transplanted after treatment. From September 18 (transplanting date) to October 27 (end of survey date), the incidence rate and incidence of bacterial wilt were investigated for all plants in each section at 10-day intervals, using different indices. The evaluation methods for disease severity, control value, and phytotoxicity were the same as in Example 1. The results are shown in Table 3, along with Comparative Example 2 (untreated section) where no pesticides were used.

[0055] [Table 3]

[0056] [Example 3] Prepare the required number of containers (57cm x 39cm x 25cm deep) in the same manner as in Example 1, and collect the tomato bacterial wilt contaminated soil (bacterial wilt bacteria are cultured in SMB medium with shaking, the collected bacterial suspension is mixed into the soil, and the bacterial wilt bacteria density in the soil is increased to 10%). 4 The soil was adjusted to cfu / g and used as the test soil. The container was filled with ( ). The test plot consisted of 8 rows of 4 plants (25 cm apart) per container. On June 16, 2025, planting holes (4 per container) large enough to accommodate 9cm pots were dug in the containers using a trowel. Agents A and B, in the proportions described in Formulation Examples 1, 2, 4, and 5, were scattered into the planting holes immediately before transplanting and lightly mixed with the soil in the holes by hand. Tomato seedlings (Momotaro 8: self-rooted cultivation) were transplanted after treatment. From July 8 (22 days after transplanting) to July 22 (end of the survey), the incidence rate and incidence of bacterial wilt were investigated for all plants in each plot approximately every week, using different indices. The evaluation methods for disease severity, control value, and phytotoxicity were the same as in Example 1. The results are shown in Table 4, along with Comparative Example 3 (untreated plot) where no pesticides were used.

[0057] [Table 4]

[0058] The results from Examples 1 to 3 indicate that the resistance inducer of the present invention has a high control effect against tomato bacterial wilt. [Industrial applicability]

[0059] The resistance-inducing agent of the present invention, which contains an alkaline earth metal peroxide such as calcium peroxide and iron or an iron-containing inorganic compound, is effective as a resistance-inducing agent against plant pathogens and is useful as a simple and stable agent that can be used for a long period of time. Furthermore, the disease control method of the present invention using this resistance-inducing agent is effective as a disease control method that has little phytotoxicity to vegetables, controls infectious diseases such as filamentous fungal diseases or bacterial diseases, and does not cause phytotoxicity to vegetables. The resistance-inducing agent against plant pathogens of the present invention can also be applied to vegetables, fruit trees, fruits, and the like. [Explanation of symbols]

[0060] 1 ridge 2. Planting holes 3 Seedling growing soil 4 roots 5 seedlings (above ground) 6 leaves 7 Stems 8. Agent A 9. Agent B 10. Soil (field) 40 Roots (after growth) 50 seedlings (aerial part, after growth) 99 Bacteria (bacterial wilt)

Claims

1. It contains the agents represented by Agent A and Agent B below, The amount of each agent applied to one plant (or seedling) of horticultural plants is shown below: Resistance-inducing agents against plant pathogens: Agent A: Preparation containing calcium peroxide or magnesium peroxide: 1 g to 100 g / pack Agent B: Iron or iron-containing inorganic compound: 1 g to 100 g / pack And, A resistance inducer comprising the above-mentioned agent A and agent B as active ingredients, wherein agent A contains 10% by mass or more and 60% by mass or less.

2. In the resistance inducer according to claim 1, the mixing ratio (mass ratio) of agent A and agent B is, A resistance inducer where the ratio of agent A to agent B is 1:0.02 to 1:

10.

3. In the resistance inducer according to claim 1, the mass of calcium peroxide or magnesium peroxide in agent A (M Ｅ The mass of iron in agent B relative to (M Ｆ ) Mass ratio M Ｆ / M Ｅ However, it is a resistance inducer whose value is between 0.01 and 2.

0.

4. A method for controlling bacterial or fungal diseases by mixing a planting hole soil with a resistance-inducing agent for plant pathogens described in any one of claims 1 to 3.

5. The method for controlling bacterial diseases according to claim 4, wherein the bacterial disease is tomato bacterial wilt.

6. The method for controlling a fungal disease according to claim 4, wherein the fungal disease is sweet potato vine disease.

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