Skin surface barrier film forming agent, skin barrier function improving agent, and skin care method

Abstract

To provide a method for reducing damage to human skin caused by external factors such as harmful substances in the air, using an active ingredient derived from algae, and an agent used in said method. [Solution] A skin surface barrier film forming agent containing sulfated polysaccharides derived from Suizenji-nori as an active ingredient; a skin barrier function improving agent containing sulfated polysaccharides derived from Suizenji-nori as an active ingredient; and a skin care method for enhancing the skin's barrier function, improving the skin's resistance to external stress, and improving the skin's anti-fouling properties, wherein the skin surface barrier film forming agent or the skin barrier function improving agent is applied to a healthy skin surface.

Description

TECHNICAL FIELD

[0001] The present invention relates to a skin surface barrier film-forming agent, a skin barrier function improving agent, and a skin care method.

BACKGROUND ART

[0002] Skin problems are caused by external factors (ultraviolet rays, dryness, atmospheric harmful substances, etc.) and internal factors (aging, disrupted hormone balance, stress, dietary habits, etc.). In recent years, along with climate change and industrial development, concerns over atmospheric harmful substances, which are one of the aforementioned external factors, have been increasing. Examples of atmospheric harmful substances include urban atmospheric dust (PM2.5; fine particulate matter), yellow sand, pollen, volatile organic compounds (including automobile exhaust gas) generated from factories, construction sites, transportation means and the like. Normally, the inherent barrier function of the skin prevents these substances from penetrating into the skin. However, when harmful substances penetrate into the skin due to a lowered barrier function or the like, various functions of the skin deteriorate, problems such as inflammation, dryness, spots, wrinkles, and acne occur, and the skin condition may be aggravated. Skin problems not only impair appearance, but also cause discomfort due to itching and pain, and may reduce QOL in combination with psychological effects. Therefore, maintaining healthy skin is an important issue for many people.

[0003] In the skin care market, demand for naturally derived components and non-animal derived components is increasing in order to avoid adverse effects of synthetic chemical substances on the skin. Examples of naturally derived components and non-animal derived components include plant-derived raw materials extracted from plant flowers, leaves, roots, fruits and the like, algae-derived raw materials extracted from seaweeds and freshwater algae, microorganism-derived raw materials obtained by fermentation of microorganisms, and the like. Sulfated polysaccharides derived from the freshwater blue-green alga Aphanothece sacrum are known to be excellent in moisturizing properties and the like, and have been proposed as raw materials for cosmetics (for example, Patent Document 1). It has been confirmed that by treating a filter with the sulfated polysaccharide derived from Aphanothece sacrum, substances contained in cigarette smoke are blocked by the filter (see Non-Patent Document 1).

REFERENCES OF THE PRIOR ART

[0004] [Patent Document 1] Patent No. 4066443 [Patent Document 2] Japanese Patent Publication No. 2023-60582 [Non-patent literature]

[0005] [Non-Patent Document 1] Doi et al., "Protective Effects of Sacran, a Natural Polysaccharide, Against Adverse Effects on the Skin Induced by Tobacco Smoke," Journal of Cosmetic Science, No. 70, Vol. 1, 2019, pp. 17-31. [Overview of the project] [Problems that the invention aims to solve]

[0006] According to the invention described in Non-Patent Document 1, sulfated polysaccharides (sacran) derived from Suizenji-nori are said to reduce stress caused by cigarette smoke, but it has not been confirmed whether they actually have an effect on harmful substances in the air on human skin. Furthermore, although the antifouling properties of sulfated polysaccharides derived from Suizenji-nori are disclosed in Patent Document 2, Patent Document 2 only confirms that sulfated polysaccharides derived from Suizenji-nori applied to a latex surface have an antifouling effect (an effect that makes dirt easier to remove), and has not confirmed the effect on human skin.

[0007] The present invention has been made in view of the above problems, and aims to provide a method for reducing damage to human skin caused by external factors such as harmful substances in the air, using an active ingredient derived from algae, and an agent used in said method. [Means for solving the problem]

[0008] The inventors of this invention conducted intensive research to solve the above problems and, as a result, novelly discovered that sulfated polysaccharides derived from Suizenji-nori can reduce skin damage caused by external stressors such as harmful substances in the air by forming a barrier film on the skin surface or improving the skin barrier function, thereby completing the present invention.

[0009] In other words, the present invention provides the following invention. (1) A skin surface barrier film-forming agent containing sulfated polysaccharides derived from Suizenji Nori as an active ingredient. (2) A skin barrier function improving agent containing sulfated polysaccharides derived from Suizenji Nori as an active ingredient. (3) A skin care method for enhancing the skin's barrier function, improving the skin's resistance to external stress, and improving the skin's anti-fouling properties, comprising applying the skin surface barrier film-forming agent of (1) or the skin barrier function improving agent of (2) to a healthy skin surface. [Effects of the Invention]

[0010] According to the present invention, by forming a barrier film on the skin surface or improving the skin's inherent barrier function, the overall skin barrier function can be enhanced, improving the skin's resistance to external stress and enhancing its stain resistance. As a result, it becomes possible to protect the skin from harmful substances in the air and keep the skin healthy. Furthermore, since Suizenji-nori-derived sulfated polysaccharides are derived from algae, they can be used safely and securely compared to synthetic ingredients derived from petroleum raw materials. In addition, Suizenji-nori-derived sulfated polysaccharides themselves are expected to have moisturizing effects, and therefore can improve and enhance various skin functions and skin conditions, as well as providing the effects mentioned above. [Modes for carrying out the invention]

[0011] The present invention will be described in more detail below. However, the present invention is not limited to the embodiments shown below. In this specification, unless otherwise specified, "parts" and "%" mean "parts by mass" and "% by mass," respectively. In this specification, unless otherwise specified, "XX or greater and YY or less" or "XX to YY" means a numerical range including the lower and upper limits which are the endpoints. When a numerical range is described in steps, the upper and lower limits of each numerical range can be combined in any way.

[0012] <Skin surface barrier film forming agent> The skin surface barrier film-forming agent of the present invention contains sulfated polysaccharides derived from Suizenji-nori and is an agent that has the effect of forming a barrier film on the skin surface. In the skin surface barrier film-forming agent, the sulfated polysaccharides derived from Suizenji-nori are contained as an active ingredient. Skin surface barrier-forming agents aim to create a protective film on the skin's surface, shielding it from external stimuli and pollutants. This strengthens the skin's barrier function, reducing the occurrence of skin problems and keeping the skin healthy.

[0013] (Sulfated polysaccharides derived from Suizenji-nori) Sulfated polysaccharides derived from Aphanothece sacrum (hereinafter sometimes simply referred to as "sulfated polysaccharides" or "polysaccharides") are a type of sulfated polysaccharide derived from the freshwater cyanobacterium Aphanothece sacrum, obtained naturally or through cultivation, and generally having an average molecular weight of 10,000,000 or more. Specifically, they are sugar derivatives having a repeating structure of sugar chain units in which sugar structures having a hexose structure and sugar structures having a pentose structure are linked in a linear or branched chain by α-glycosidic bonds or β-glycosidic bonds, wherein the sugar chain units contain sulfated sugar as sugar structures, and in the sugar chain units, 2.7 or more hydroxyl groups per 100 hydroxyl groups are sulfated, or sulfur element accounts for 1.5% by mass or more of the total elements.

[0014] Specific examples of sulfated polysaccharides include sulfated muramic acid or sulfated N-acetylmuramic acid.

[0015] The sulfated polysaccharide includes at least glucose, galactose, mannose, galactosamine, xylose, arabinose, glucuronic acid, galacturonic acid, fucose, and rhamnose, and a functional group selected from the functional group group including at least a sulfate group, a lactate group, and a methyl group may be bonded to any optional bonding position on these sugar structures. The sulfate group, which is a characteristic of the sulfated polysaccharide derived from Aphanothece sacrum, is preferably contained in the sulfated polysaccharide in an amount of 3 to 20 mass%, more preferably 5 to 15 mass%, and even more preferably 8 to 12 mass%.

[0016] A part of the sugar structures constituting the sulfated polysaccharide may further be bonded to a peptide or a lipid.

[0017] The molar ratio of the main sugar structures constituting the sulfated polysaccharide may be arabinose 1.1 : fucose 3.7 : rhamnose 15.4 : xylose 17.0 : mannose 10.5 : galactose 12.3 : galacturonic acid 4.6 : glucuronic acid 4.7 : glucose 28.8 : galactosamine 2.03. In addition, the number of types of sugar structures (monosaccharides) constituting the sulfated polysaccharide may be 5 or more, 7 or more, 8 or more, or 10 or more.

[0018] The average molecular weight of the sulfated polysaccharide is, for example, 500,000 or more, 800,000 or more, 1,000,000 or more, 2,000,000 or more, 5,000,000 or more, 10,000,000 or more, 20,000,000 or more, etc., and is 30,000,000 or less, 28,000,000 or less, 25,000,000 or less, 22,000,000 or less, etc. Non-contradictory combinations of these values are also acceptable, for example, 1,000,000 to 30,000,000, 5,000,000 to 28,000,000, 10,000,000 to 25,000,000, 20,000,000 to 22,000,000, etc.

[0019] The sulfated polysaccharide may be one that has been extracted, isolated, and purified from Aphanothece sacrum. The method for extracting the sugar derivative from Aphanothece sacrum is not particularly limited, and examples thereof include conventional methods as also described in Patent Document 2. Specifically, for example, the following may be mentioned: An appropriate amount of Aphanothece sacrum is frozen and thawed, then washed with water to remove water-soluble pigments, then the fat-soluble pigments are removed with stirring for a whole day and night using an organic solvent such as ethanol, and after the organic solvent is separated from the pigment-removed Aphanothece sacrum, the mixture is heated, for example, to 40 to 80°C in, for example, a 0.1 to 0.6 N aqueous sodium hydroxide solution, and dissolved with stirring for, for example, 5 to 12 hours. The aqueous solution of the sugar derivative extracted by this operation is neutralized with, for example, concentrated hydrochloric acid. Here, desalination may or may not be performed by, for example, immersing the product in 60 to 80% isopropyl alcohol. Thereafter, the mixture is poured into, for example, 100% isopropanol with stirring to precipitate a gel-like sugar derivative, followed by drying with hot air. The drying with hot air may be performed at, for example, 85 to 100°C for, for example, 6 to 24 hours. The extracted and precipitated sugar derivative may be purified by centrifugation, filtration, alcohol washing, or the like.

[0020] As the sulfated polysaccharide, a commercially available product may be used, and examples of the commercially available product include "SACRAN" (registered trademark) manufactured by Green Science Material (GSM) Inc., and "SACRANEX" (registered trademark) manufactured by the DIC Group.

[0021] Further, as long as the effects of the present invention are exhibited, the sulfated polysaccharide may be in a form contained in a crushed product of a dried Aphanothece sacrum (hereinafter referred to as "crushed dried Aphanothece sacrum product"). Accordingly, the present invention can also provide a skin surface barrier film-forming agent containing, as an active ingredient, the crushed dried Aphanothece sacrum product containing the sulfated polysaccharide. Examples of the crushed dried Aphanothece sacrum product containing the sugar derivative may be a crushed freeze-dried Aphanothece sacrum product containing the sugar derivative, a crushed sun-dried Aphanothece sacrum product containing the sugar derivative, or a crushed hot-air-dried Aphanothece sacrum product containing the sugar derivative, and any one of these may be used, or a plurality thereof may be used in combination. Crushed dried Suizenji-nori containing sulfated polysaccharides can all be prepared by conventional methods. For example, crushed freeze-dried Suizenji-nori containing the aforementioned sugar derivatives can be prepared by freezing Suizenji-nori containing water in a low-temperature freezer, removing the water contained within the algae by vacuum freeze-drying to obtain freeze-dried Suizenji-nori, and then crushing it (for example, by grinding it with a mortar and pestle, or by grinding it into a powder using a pulverizer). After that, it may be suspended in an appropriate solvent or solution.

[0022] Furthermore, the sulfated polysaccharides may be present in an extract of Suizenji-nori (hereinafter referred to as "Suizenji-nori extract") insofar as they achieve the effects of the present invention. Therefore, the present invention can also provide a skin surface barrier film-forming agent containing a Suizenji Nori extract containing sulfated polysaccharides as an active ingredient. Suizenji-nori extract containing sulfated polysaccharides can be prepared, for example, by the method described above.

[0023] The skin surface barrier film-forming agent of the present invention is an agent that has the effect of forming a barrier film on the skin surface. Basically, the applied component alone (the skin surface barrier film-forming agent alone, or the formulation alone if the skin surface barrier film-forming agent is incorporated into cosmetics, etc.) forms a barrier film on the skin surface, but some component in the applied component may interact with skin cells in some way. Interactions include adsorption, chemical reactions, ion exchange, penetration, etc. The target skin cells may be cells present in the epidermis or cells present in the dermis, but when the skin surface barrier film-forming agent of the present invention is used in cosmetics, it is particularly preferable to target the epidermis. Examples of cells present in the epidermis include keratinocytes, granule cells, spinous cells, Langerhans cells, and basal cells. Examples of cells present in the dermis include fibroblasts and pigment cells (melanocytes).

[0024] The skin surface barrier film-forming agent of the present invention may be used as is, but it may also be incorporated into cosmetics, topical skin preparations, quasi-drugs, pharmaceuticals, etc., for the purpose of imparting a skin surface barrier film-forming effect. In particular, its use in cosmetics or topical skin preparations is preferred.

[0025] • Cosmetics or topical skin preparations When the skin surface barrier film-forming agent of the present invention is used in cosmetics, topical skin preparations, etc., the cosmetics, topical skin preparations, etc. can be manufactured by conventional methods (for example, methods described in the Japanese Pharmacopoeia) by formulating a skin surface barrier film-forming agent containing sulfated polysaccharides derived from Suizenji Nori as an active ingredient, and optionally mixing it with other ingredients.

[0026] Other components include pharmaceutically acceptable carriers. A "pharmaceutically acceptable carrier" means a carrier that does not inhibit the physiological activity of the active ingredient and does not exhibit substantial toxicity to the target of use. "Substantial toxicity" means that the ingredient does not exhibit toxicity to the target of use at the amount normally used. Pharmaceutically acceptable carriers are not particularly limited and include oily components, humectants, texture enhancers, surfactants, various polymers, thickeners, gelling agents, antioxidants, preservatives, antibacterial agents, chelating agents, pH adjusters, acids and alkalis, powders, UV absorbers, various active ingredients (whitening agents, anti-inflammatory agents, hair growth agents, blood circulation promoters, stimulants, anti-aging agents, etc.), antipruritics, vitamins and their derivatives, hormones, various extracts, fragrances, colorants (pigments, colorants, dyes, pigments), etc.

[0027] Oily components include higher alcohols such as cetanol, myristyl alcohol, oleyl alcohol, lauryl alcohol, cetostearyl alcohol, stearyl alcohol, arachidodecanol, behenyl alcohol, jojoba alcohol, chimyl alcohol, cerachidodecanol, batyl alcohol, hexyldecanol, isostearyl alcohol, 2-octyldodecanol, and dimergol; aralkyl alcohols and their derivatives such as benzyl alcohol; lauric acid, myristic acid, palmitic acid, stearic acid, and isostearic acid. , behenic acid, undecylenic acid, 12-hydroxystearic acid, palmitoleic acid, oleic acid, linoleic acid, erucic acid, docosahexaenoic acid, eicosapentaenoic acid, isohexadecanoic acid, anteisohenicosanic acid, long-chain branched fatty acids, dimer acids, hydrogenated dimer acids and other higher fatty acids, and their aluminum salts, calcium salts, magnesium salts, zinc salts, sodium salts, potassium salts and other metal soaps, and amine salts such as ethanolamine salts, and nitrogen-containing derivatives such as amides; liquid paraffin (mineral oil), heavy liquid ion Carbides such as soparaffins, light liquid isoparaffins, α-olefin oligomers, polyisobutenes, hydrogenated polyisobutenes, polybutenes, squalanes, olive-derived squalanes, squalenes, petrolatum, and solid paraffins; waxes such as candelilla wax, carnauba wax, rice wax, wood wax, beeswax, montan wax, ozokerite, ceresin, paraffin wax, microcrystalline wax, petrolatum, Fischer-Tropsch wax, polyethylene wax, and ethylene-propylene copolymers; coconut oil, Vegetable oils such as palm oil, palm kernel oil, safflower oil, olive oil, castor oil, avocado oil, sesame oil, tea oil, evening primrose oil, wheat germ oil, macadamia nut oil, hazelnut oil, kukui nut oil, rosehip oil, meadowfoam oil, peach kernel oil, tea tree oil, peppermint oil, corn oil, rapeseed oil, sunflower oil, wheat germ oil, linseed oil, cottonseed oil, soybean oil, peanut oil, rice bran oil, cocoa butter, shea butter, hydrogenated coconut oil, hydrogenated castor oil, jojoba oil, hydrogenated jojoba oil, etc.; animal fats such as beef tallow, milk fat, horse fat, egg yolk oil, mink oil, turtle oil, etc.Animal waxes such as whale wax, lanolin, and orange roughy oil; lanolin compounds such as liquid lanolin, reduced lanolin, adsorbed and purified lanolin, lanolin acetate, liquid lanolin acetate, hydroxylanolin, polyoxyethylene lanolin, lanolin fatty acids, hard lanolin fatty acids, lanolin alcohol, lanolin acetate alcohol, and (cetyl lanolyl) acetate; lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylglycerol Phosphatidylinositol, sphingomyelin and other sphingophospholipids, phosphatidic acid, lysolecithin and other phospholipids; phospholipid derivatives such as hydrogenated soybean phospholipid, partially hydrogenated soybean phospholipid, hydrogenated egg yolk phospholipid, partially hydrogenated egg yolk phospholipid; cholesterol, dihydrocholesterol, lanosterol, dihydrolanosterol, phytosterol, cholic acid and other sterols; sapogenins; saponins; cholesteryl acetate, cholesteryl nonanoate Cholesteryl stearate, cholesteryl isostearate, cholesteryl oleate, N-lauroyl-L-glutamic acid di(cholesteryl / behenyl / octyldodecyl), N-lauroyl-L-glutamic acid di(cholesteryl / octyldodecyl), N-lauroyl-L-glutamic acid di(phytosteryl / behenyl / octyldodecyl), N-lauroyl-L-glutamic acid di(phytosteryl / octyldodecyl), N-lauroyl sarcosinate isopropyl Sterol esters such as acyl sarcosinate alkyl esters, cholesteryl 12-hydroxystearate, macadamia nut oil fatty acid cholesteryl, macadamia nut oil fatty acid phytosteryl, phytosteryl isostearate, soft lanolin fatty acid cholesteryl, hard lanolin fatty acid cholesteryl, long-chain branched fatty acid cholesteryl, long-chain α-hydroxy fatty acid cholesteryl; lipid complexes such as phospholipid-cholesterol complexes and phospholipid-phytosterol complexes;Octyldodecyl myristate, hexyldecyl myristate, octyldodecyl isostearate, cetyl palimitate, octyldodecyl palmitate, cetyl octanoate, hexyldecyl octanoate, isotridecyl isononanoate, isononyl isononanoate, octyl isononanoate, isotridecyl isononanoate, isodecyl neopentanoate, isotridecyl neopentanoate, isostearyl neopentanoate, octyldodecyl neodecanoate, oleyl oleate, octyldodecyl oleate, ricinoleic acid Monoalcoholic carboxylic acid esters such as octyldodecyl, octyldodecyl lanolate, hexyldecyl dimethyloctanoate, octyldodecyl erucate, hydrogenated castor oil isostearate, ethyl oleate, ethyl avocado oil fatty acid, isopropyl myristate, isopropyl palmitate, octyl palmitate, isopropyl isostearate, isopropyl lanolate, triethyl citrate; oxyacid esters such as cetyl lactate, diisostearyl malate, hydrogenated castor oil monoisostearate;Glyceryl trioctanoate, glyceryl trioleate, glyceryl triisostearate, glyceryl diisostearate, caprylic / capric triglyceride, caprylic / capric / myristic / stearic triglyceride, hydrogenated rosin triglyceride (hydrogenated ester gum), rosin triglyceride (ester gum), glyceryl eicosanedioate behenate, glyceryl tetradecanediol / eicosanedioate, polyglyceryl-10 tetradecanediol / eicosanedioate, trimethylolpropane trioctanoate, trimethylolpropane triisostearate, neopentyl glycol dioctanoate, neopentyl glycol dicaprate, 2-butyl-2-ethyl-1,3-propanediol dioctanoate, 3-methyl-1,5-pentanediol dineopentanoate Polyhydric alcohol fatty acid esters such as 2,4-diethyl-1,5-pentanediol dineopentanoate, propylene glycol dioleate, pentaerythrityl tetraoctanoate, hydrogenated rosin pentaerythrityl, ditrimethylolpropane triethylhexanoate, ditrimethylolpropane (isostearate / sebacate), pentaerythrityl triethylhexanoate, dipentaerythrityl (hydroxystearate / stearate / rosinate), diglyceryl diisostearate, polyglyceryl tetraisostearate, polyglyceryl-10 nonaisostearate, polyglyceryl-8 dec(erucate / isostearate / ricinoleate), diglyceryl oligoester (hexyldecanoate / sebacate), glycol distearate (ethylene glycol distearate);Diisopropyl dimer dilinoleate, diisostearyl dimer dilinoleate, di(isostearyl / phytosteryl) dimer dilinoleate, (phytosteryl / behenyl) dimer dilinoleate, (phytosteryl / isostearyl / cetyl / stearyl / behenyl) dimer dilinoleate, dimer dilinoleyl bis(phytosteryl / behenyl / isostearyl) dimer dilinoleate, dimer dilinoleyl diisostearate, hydrogenated rosin condensate of dimer dilinoleate, hydrogenated castor oil dimer dilinoleate, hydroxyalkylated Dimer acid or dimer ol derivatives such as imergerinoleyl ether; fatty acid alkanolamides such as coconut oil fatty acid monoethanolamide (cocamide MEA), coconut oil fatty acid diethanolamide (cocamide DEA), lauric acid monoethanolamide (lauramide MEA), lauric acid diethanolamide (lauramide DEA), lauric acid monoisopropanolamide (lauramide MIPA), palmitic acid monoethanolamide (palmitamide MEA), palmitic acid diethanolamide (palmitamide DEA), and coconut oil fatty acid methylethanolamide (cocamide methyl MEA);Dimethicone (dimethylpolysiloxane), highly polymerized dimethicone (highly polymerized dimethylpolysiloxane), cyclomethicone (cyclic dimethylsiloxane, decamethylcyclopentasiloxane), phenyl trimethicone, diphenyl dimethicone, phenyl dimethicone, stearoxypropyl dimethylamine, (aminoethylaminopropyl methicone / dimethicone) copolymer, dimethiconol, dimethiconol crosspolymer, silicone resin, silicone rubber, amino-modified silicones such as aminopropyl dimethicone and amodimethicone, cationic-modified silicones, polyether-modified silicones such as dimethicone copolyol, polyglycerides Silicones such as phosphorus-modified silicones, sugar-modified silicones, carboxylic acid-modified silicones, phosphate-modified silicones, sulfuric acid-modified silicones, alkyl-modified silicones, fatty acid-modified silicones, alkyl ether-modified silicones, amino acid-modified silicones, peptide-modified silicones, fluorine-modified silicones, cation-modified and polyether-modified silicones, amino-modified and polyether-modified silicones, alkyl-modified and polyether-modified silicones, and polysiloxane-oxyalkylene copolymers are preferred. Fluorine-based oils such as perfluorodecane, perfluorooctane, and perfluoropolyether are also preferred.

[0028] As humectants and texture enhancers, these include: glycerin, 1,3-butylene glycol, propylene glycol, 3-methyl-1,3-butanediol, 1,3-propanediol, 2-methyl-1,3-propanediol, trimethylolpropane, pentaerythritol, hexylene glycol, diglycerin, polyglycerin, diethylene glycol, polyethylene glycol, dipropylene glycol, polypropylene glycol, ethylene glycol-propylene glycol copolymers and other polyols and their polymers; glycol alkyl ethers such as diethylene glycol monoethyl ether (ethoxydiglycol), ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, and diethylene glycol dibutyl ether; sugar alcohols such as sorbitol, xylitol, erythritol, mannitol, and maltitol; glucose, fructose, galactose, mannose, threose, xylose, arabinose, fucose, ribose, deoxyribose, maltose, trehalose, lactose, Natural sugars and their derivatives such as raffinose, gluconic acid, glucuronic acid, β-glucan, chitin, chitosan, heparin and derivatives, pectin, arabinogalactan, dextrin, dextran, glycogen, ethyl glucoside, glucosylethyl methacrylate polymer or copolymer; hyaluronic acid, sodium hyaluronate; sodium chondroitin sulfate; mucoitin sulfate, caronin sulfate, kerato sulfate, dermatan sulfate; Tremella fuciformis extract, Tremella fuciformis polysaccharide; fucoidan; tuberose polysaccharide, etc. Derived polysaccharides; organic acids such as citric acid, tartaric acid, and lactic acid and their salts; urea; 2-pyrrolidone-5-carboxylic acid and its sodium salt and other salts; amino acids such as betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, tyrosine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, tryptophan, histidine, and taurine and their salts;Protein peptides and their derivatives such as collagen, fish-derived collagen, atelocollagen, gelatin, elastin, collagen-degraded peptides, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin-degraded peptides, keratin-degraded peptides, hydrolyzed keratin, conchiolin-degraded peptides, hydrolyzed conchiolin, silk protein-degraded peptides, hydrolyzed silk, sodium lauroyl hydrolyzed silk, soy protein-degraded peptides, wheat protein-degraded peptides, hydrolyzed wheat protein, casein-degraded peptides, acylated peptides, etc.; palmitoyl oligopeptides, palmitoyl pentapeptides, palmitoyl tetrapeptides, etc. Preferred ingredients include ceramides; silylated peptides; lactic acid bacteria culture solution, yeast extract, eggshell membrane protein, bovine submandibular gland mucin, hypotaurine, sesamin glycosides, glutathione, albumin, whey; choline chloride, phosphorylcholine; placental extract, aerlastin, collagen, witch hazel water, loofah water, chamomile extract, licorice extract, comfrey extract, silk extract, rose extract, yarrow extract, eucalyptus extract, sweet clover extract, and other animal and plant extracts; natural ceramides (types 1, 2, 3, 4, 5, 6), hydroxyceramides, pseudoceramides, sphingoglycolipids, ceramides, and ceramide-containing extracts; and other ceramides.

[0029] Preferred surfactants include anionic surfactants, nonionic surfactants, cationic surfactants, amphoteric surfactants, and polymeric surfactants. There are no particular restrictions on the HLB of the surfactant; those with a low HLB of around 1 to a high HLB of around 20 can be used, and it is also preferable to combine those with low and high HLB values. Examples of preferred surfactants include anionic surfactants such as fatty acid salts like potassium laurate and potassium myristate; alkyl sulfate esters such as sodium lauryl sulfate, triethanolamine lauryl sulfate, and ammonium lauryl sulfate; polyoxyethylene alkyl sulfates such as sodium laureth sulfate and triethanolamine laureth sulfate; acyl N-methyl amino acid salts such as sodium cocoyl methyl taurate, potassium cocoyl methyl taurate, sodium lauroyl methyl taurate, sodium myristoyl methyl taurate, sodium lauroyl methylalanine, sodium lauroyl sarcosinate, triethanolamine lauroyl sarcosinate, and sodium lauroyl glutamate methylalanine; sodium cocoyl glutamate, triethanolamine cocoyl glutamate, sodium lauroyl glutamate, sodium myristoyl glutamate, sodium stearoyl glutamate, and palmitoyl glutamate. Acyl amino acid salts such as ditriethanolamine aspartate and triethanolamine cocoyl alanine; polyoxyethylene alkyl ether acetates such as sodium laureth acetate; succinate ester salts such as sodium lauroyl monoethanolamide succinate; fatty acid alkanolamide ether carboxylates; acyl lactates; polyoxyethylene fatty acid amine sulfates; fatty acid alkanolamide sulfates; fatty acid glyceride sulfates such as sodium hydrogenated coconut oil fatty acid glycerin sulfate; alkylbenzene polyoxyethylene sulfates; olefin sulfonates such as sodium α-olefin sulfonate; alkyl sulfosuccinates such as disodium lauryl sulfosuccinate and sodium dioctyl sulfosuccinate; alkyl ether sulfosuccinates such as disodium laureth sulfosuccinate, monolauroyl monoethanolamide polyoxyethylene sulfosuccinate sodium, and sodium lauryl polypropylene glycol sulfosuccinate sodium;Alkylbenzenesulfonates such as sodium tetradecylbenzenesulfonate and triethanolamine tetradecylbenzenesulfonate; alkylnaphthalene sulfonates; alkanesulfonates; α-sulfo fatty acid methyl ester salts; acyl isethionates; alkyl glycidyl ether sulfonates; alkyl sulfoacetates; alkyl ether phosphate salts such as sodium laureth phosphate, sodium dilaureth phosphate, sodium trilaureth phosphate, and sodium monooleth phosphate; alkyl phosphate salts such as potassium lauryl phosphate; sodium caseinate; alkylaryl ether phosphates; fatty acid amide ether phosphates; phospholipids such as phosphatidylglycerol, phosphatidylinositol, and phosphatidic acid; silicone-based anionic surfactants such as carboxylic acid-modified silicones, phosphate-modified silicones, and sulfate-modified silicones; nonionic surfactants include laureth (polyoxyethylene lauryl ether) and ceteth (polyoxyethylene lauryl ether). Polyoxyethylene alkyl ethers of various polyoxyethylene addition numbers, such as polyoxyethylene cetyl ethers, steareths (polyoxyethylene stearyl ethers), beheneths (polyoxyethylene behenyl ethers), isosteareths (polyoxyethylene isostearyl ethers), and octyldodeceths (polyoxyethylene octyldodecyl ethers); polyoxyethylene alkylphenyl ethers; castor oil and hydrogenated castor oil derivatives such as polyoxyethylene hydrogenated castor oil, polyoxyethylene castor oil monoisostearate, polyoxyethylene hydrogenated castor oil triisostearate, polyoxyethylene hydrogenated castor oil monopyroglutamic acid monoisostearate diester, and polyoxyethylene hydrogenated castor oil maleic acid; polyoxyethylene phytosterol; polyoxyethylene cholesterol; polyoxyethylene cholestanol; polyoxyethylene lanolin; polyoxyethylene reduced lanolin;Polyoxyethylene / polyoxypropylene alkyl ethers such as polyoxyethylene / polyoxypropylene cetyl ether, polyoxyethylene / polyoxypropylene 2-decyltetradecyl ether, polyoxyethylene / polyoxypropylene monobutyl ether, polyoxyethylene / polyoxypropylene hydrogenated lanolin, polyoxyethylene / polyoxypropylene glycerin ether; polyoxyethylene / polyoxypropylene glycol; (poly) such as PPG-9 diglyceryl. ) Glycerin polyoxypropylene glycol; glyceryl stearate, glyceryl isostearate, glyceryl palmitate, glyceryl myristate, glyceryl oleate, coconut oil fatty acid glyceryl, monocottonseed oil fatty acid glycerin, monoerucate glycerin, sesquioleate glycerin, α,α'-oleate pyroglutamate glycerin, monostearate glycerin malic acid and other glycerin fatty acid partial esters; polyglyceryl-2, 3, 4, 5, 6, 8 stearate , same 10, polyglyceryl-6 distearate, same 10, polyglyceryl-2 tristearate, polyglyceryl-10 decaisostearate, polyglyceryl-2 isostearate, same 3, same 4, same 5, same 6, same 8, same 10, polyglyceryl-2 diisostearate (diglyceryl diisostearate), same 3, same 10, polyglyceryl-2 triisostearate, polyglyceryl-2 tetraisostearate, polyglyceryl-10 decaisostearate, polyglyceryl-2 oleate, same 3, Polyglycerin fatty acid esters such as Polyglyceryl-6 dioleate, Polyglyceryl-2 trioleate, Polyglyceryl-10 decaoleate; Ethylene glycol monofatty acid esters such as Ethylene glycol monostearate; Propylene glycol monofatty acid esters such as Propylene glycol monostearate; Pentaerythritol partial fatty acid ester; Sorbitol partial fatty acid ester; Maltitol partial fatty acid ester; Maltitol ether;Sorbitan fatty acid esters such as sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, sorbitan penta-2-ethylhexyl diglycerol sorbitan, sorbitan tetra-2-ethylhexyl diglycerol sorbitan; sugar derivative partial esters such as sucrose fatty acid esters, methyl glucoside fatty acid esters, and trehalose undecylenate; alkyl glucosides such as caprylyl glucoside; alkyl polyglycerides Coside; lanolin alcohol; reduced lanolin; polyoxyethylene fatty acid mono and diesters such as polyoxyethylene distearate, polyethylene glycol diisostearate, polyoxyethylene monooleate, polyoxyethylene dioleate; polyoxyethylene propylene glycol fatty acid esters; polyoxyethylene glycerin fats such as polyoxyethylene monooleate, polyoxyethylene glycerin monoisostearate, polyoxyethylene glycerin triisostearate, and other polyoxyethylene monooleates. Fatty acid esters; polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, and polyoxyethylene sorbitan tetraoleate; polyoxyethylene sorbitol fatty acid esters such as polyoxyethylene sorbitol monolaurate, polyoxyethylene sorbitol monooleate, polyoxyethylene sorbitol pentaoleate, and polyoxyethylene sorbitol monostearate; polyoxyethylene methyl glucoside fatty acid esters; polyoxyethylene alkyl ether fatty acid esters; polyoxyethylene animal and vegetable oils and fats such as polyoxyethylene sorbitol beeswax; alkyl glyceryl ethers such as isostearyl glyceryl ether, chymyl alcohol, cerakyl alcohol, and batyl alcohol; polyhydric alcohol alkyl ethers; polyoxyethylene alkylamines; tetrapolyoxyethylene-tetrapolyoxypropylene-ethylenediamine condensates; natural surfactants such as saponins and sophorolipids; polyoxyethylene fatty acid amides;Fatty acid alkanolamides such as coconut oil fatty acid monoethanolamide (cocamide MEA), coconut oil fatty acid diethanolamide (cocamide DEA), lauric acid monoethanolamide (lauramide MEA), lauric acid diethanolamide (lauramide DEA), lauric acid monoisopropanolamide (lauramide MIPA), palmitic acid monoethanolamide (palmitamide MEA), palmitic acid diethanolamide (palmitamide DEA), coconut oil fatty acid methylethanolamide (cocamide methyl MEA); lauramine oxide nonionic silicone surfactants such as: alkyldimethylamine oxides such as cocamine oxide, stearamine oxide, and behenamine oxide; alkylethoxydimethylamine oxide; polyoxyethylene alkyl mercaptan; polyether-modified silicones such as dimethicone copolyol, polysiloxane-oxyalkylene copolymers, polyglycerin-modified silicones, sugar-modified silicones, etc.; cationic surfactants such as behentrimonium chloride, steartrimonium chloride, cetrimonium chloride, lauryltrimonium Alkyltrimethylammonium chlorides such as nium chloride; alkyltrimethylammonium bromides such as stearyltrimonium bromide; dialkyldimethylammonium chlorides such as distearyldimonium chloride and dicocodimonium chloride; fatty acid amide amines and their salts such as stearamidopropyldimethylamine and stearamidoethyldiethylamine; alkyl etheramines and their salts or quaternary salts such as stearoxypropyldimethylamine; fatty acid amide-type quaternary ammonium salts such as ethyl sulfate long-chain branched fatty acid (12-31) aminopropylethyldimethylammonium and ethyl sulfate lanolin fatty acid aminopropylethyldimethylammonium; polyoxyethylene alkylamines and their salts or quaternary salts; alkylamine salts; fatty acid amide guanidium salts; alkyl etheramine mononium salts; alkyl trialkylene glycol ammonium salts; benzalkonium salts; benzethonium salts; pyridinium salts such as cetylpyridinium chloride; imidazolinium salts; alkylisoquinolinium salts; dialkylmolyphonium salts; polyamine fatty acid derivatives;Silicone-based cationic surfactants such as aminopropyl dimethicone and amodimethicone (amino-modified silicones), cationic-modified silicones, cationic and polyether-modified silicones, amino-modified and polyether-modified silicones, etc.; amphoteric surfactants such as N-alkyl-N,N-dimethylamino acid betaines such as lauryl betaine (lauryldimethylaminoacetic acid betaine); fatty acid amide alkyl-N,N-dimethylamino acid betaines such as cocamidopropyl betaine and lauramidopropyl betaine; imidazoline-type betaines such as sodium cocoamphoacetate and sodium lauroamphoacetate; alkyl sulfobetaines such as alkyldimethyl taurine; sulfate-type betaines such as alkyldimethylaminoethanol sulfate; phosphate-type betaines such as alkyldimethylaminoethanol phosphate; sphingolin lipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin; Phospholipids such as lysolecithin, hydrogenated soybean phospholipid, partially hydrogenated soybean phospholipid, hydrogenated egg yolk phospholipid, partially hydrogenated egg yolk phospholipid, and hydroxylated lecithin; silicone-based amphoteric surfactants; and polymeric surfactants such as polyvinyl alcohol, sodium alginate, starch derivatives, tragacanth gum, acrylic acid / alkyl methacrylate copolymer, and 2-methachloroyloxyethyl phosphorylcholine / stearyl methacrylate copolymer; and various silicone-based surfactants are preferred.

[0030] Various polymers, thickeners, and gelling agents include guar gum, locust bean gum, queen seed gum, carrageenan, galactan, gum arabic, tara gum, tamarind, fercerelan, karaya gum, okra gum, tragacanth gum, pectin, pectic acid and its salts, alginic acid and its salts, mannan; starches from rice, corn, potato, wheat, etc.; xanthan gum, dextran, succinoglucan, curdlan, hyaluronic acid and its salts, xanthan gum, pullulan, gellan gum, chitin, chitosa Cellulose, agar, cassia seed extract, chondroitin sulfate, casein, collagen, gelatin, albumin; methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose and its sodium salts, methylhydroxypropylcellulose, sodium cellulose sulfate, dialkyldimethylammonium sulfate, crystalline cellulose, cellulose powder, and other cellulose and its derivatives; soluble starch, carboxymethyl starch, methylhydroxypropyl starch, methyl starch, and other derivatives Starch derivatives such as corn starch aluminum octenyl succinate, hydroxypropyltrimonium chloride, and octenyl succinate; alginic acid derivatives such as sodium alginate and propylene glycol alginate; polyvinylpyridone (PVP), polyvinyl alcohol (PVA), vinylpyridone-vinyl alcohol copolymer, and polyvinyl methyl ether; polyethylene glycol, polypropylene glycol, and polyoxyethylene-polyoxypropylene copolymer; amphoteric methacrylate copolymers such as (methacryloyloxyethyl carboxybetaine / alkyl methacrylate) copolymer and (acrylates / stearyl acrylate / ethylamine oxide methacrylate) copolymer; (dimethicone / vinyl dimethicone) crosspolymer, (alkyl acrylate / diacetone acrylamide) copolymer, and (alkyl acrylate / diacetone acrylamide) copolymer AMP; partially saponified polyvinyl acetate and maleic acid copolymer; vinylpyrrolidone-dialkylaminoalkyl methacrylate copolymer; acrylic resin alkanolamine; polyesters and water-dispersible polyesters.Polyacrylamide; polyacrylic acid ester copolymers such as ethyl polyacrylate, carboxyvinyl polymers, salts such as polyacrylic acid and its sodium salt, acrylic acid / methacrylic acid ester copolymers; acrylic acid / alkyl methacrylate copolymers; cationized cellulose such as polyquaternium-10, diallyldimethylammonium chloride / acrylamide copolymers such as polyquaternium-7, acrylic acid / diallyldimethylammonium chloride copolymers such as polyquaternium-22, acrylic acid / diallyldimethylammonium chloride / acrylamide copolymers such as polyquaternium-39, acrylic acid / cationized methacrylic acid ester copolymers, acrylic acid / cationized methacrylamide copolymers, acrylic acid / methyl acrylate / methacrylamidopropyltrimethylammonium chloride copolymers such as polyquaternium-47, choline methacrylate ester polymers; cationized oligosaccharides, cationized dextran, cationized guar hydroxypropyltrimonium chloride, etc. Polysaccharides; polyethyleneimines; cationic polymers; copolymers of 2-methacryloyloxyethyl phosphorylcholine polymers such as polyquaternium-51 and butyl methacrylate copolymers; polymer emulsions such as acrylic resin emulsions, ethyl polyacrylate emulsions, polyacrylic alkyl ester emulsions, polyvinyl acetate resin emulsions, natural rubber latex, and synthetic latex; nitrocellulose; polyurethanes and various copolymers; various silicones; various silicone-based copolymers such as acrylic-silicone graft copolymers; various fluorine-based polymers; 12-hydroxystearic acid and its salts; dextrin fatty acid esters such as dextrin palmitate and dextrin myristate; anhydrous silicic acid, fuzzy silica (ultrafine particle anhydrous silicic acid), magnesium aluminum silicate, magnesium sodium silicate, metal soaps, dialkyl phosphate metal salts, bentonite, hectorite, organically modified clay minerals, sucrose fatty acid esters, and fructooligosaccharide fatty acid esters are among the preferred materials.

[0031] Preferred antioxidants include alpha-lipoic acid, salts of alpha-lipoic acid, and derivatives of alpha-lipoic acid; tocopherol derivatives such as tocopherol (vitamin E), tocotrienol, and tocopherol acetate; BHT, BHA; gallic acid derivatives such as propyl gallate; vitamin C (ascorbic acid) and / or its derivatives; erythorbic acid and its derivatives; sulfites such as sodium sulfite; bisulfites such as sodium bisulfite; thiosulfates such as sodium thiosulfate; metabisulfites; thiotaurine, hypotaurine; thioglycerol, thiourea, thioglycolic acid, and cysteine ​​hydrochloride. Preferred reducing agents include thioglycolic acid, cysteine, and cysteamine. Preferred oxidizing agents include hydrogen peroxide, ammonium persulfate, sodium bromate, and percarbonate.

[0032] As preservatives and antibacterial agents, hydroxybenzoic acids and their salts or esters such as methylparaben, ethylparaben, propylparaben, and butylparaben; salicylic acid; sodium benzoate; phenoxyethanol; 1,2-diols such as 1,2-pentanediol and 1,2-hexanediol; isothiazolinone derivatives such as methylchloroisothiazolinone and methylisothiazolinone; imidazolinium urea; dehydroacetic acid and its salts; phenols; halogenated bisphenols such as triclosan; acid ammonium compounds Preferred materials include dihydrogen ammonium compounds, quaternary ammonium salts; trichlorocarbanides, zinc pyrithione, benzalkonium chloride, benzethonium chloride, sorbic acid, chlorhexidine, chlorhexidine gluconate, halocarban, hexachlorophene, hinokitiol; phenols, isopropylphenol, cresol, thymol, parachlorophenol, phenylphenol, sodium phenylphenol, and other phenols; phenylethyl alcohol, photosensitizers, antibacterial zeolites, and silver ions.

[0033] Preferred chelating agents include edetates (ethylenediaminetetraacetates) such as EDTA, EDTA2Na, EDTA3Na, and EDTA4Na; hydroxyethylethylenediaminetriacetates such as HEDTA3Na; pentetates (diethylenetriaminepentaacetate); phytic acid; phosphonic acids such as etidronic acid and their sodium salts; sodium oxalate; polyamino acids such as polyaspartic acid and polyglutamic acid; sodium polyphosphate, sodium metaphosphate, phosphoric acid; sodium citrate, citric acid, alanine, dihydroxyethylglycine, gluconic acid, ascorbic acid, succinic acid, and tartaric acid.

[0034] Preferred pH adjusters and acids / alkalis include citric acid, sodium citrate, lactic acid, sodium lactate, glycolic acid, succinic acid, acetic acid, sodium acetate, malic acid, tartaric acid, fumaric acid, phosphoric acid, hydrochloric acid, sulfuric acid, monoethanolamine, diethanolamine, triethanolamine, isopropanolamine, triisopropanolamine, 2-amino-2-methyl-1,3-propanediol, 2-amino-2-hydroxymethyl-1,3-propanediol, arginine, sodium hydroxide, potassium hydroxide, aqueous ammonia, guanidine carbonate, and ammonium carbonate.

[0035] Powders include mica, talc, kaolin, sericite, montmorillonite, kaolinite, mica, muscovite, phlogopite, synthetic mica, red mica, biotite, vermiculite, magnesium carbonate, calcium carbonate, aluminum silicate, barium silicate, calcium silicate, magnesium silicate, strontium silicate, tungstate metal salts, magnesium, zeolite, barium sulfate, calcined calcium sulfate, calcium phosphate, fluorapatite, hydroxyapatite, ceramic powder, bentonite, smectite, clay, mud, metal soaps (e.g., zinc myristate, calcium palmitate, aluminum stearate), calcium carbonate, red iron oxide, yellow iron oxide, black iron oxide, ultramarine, Prussian blue, carbon black, titanium dioxide, fine and ultrafine titanium dioxide, zinc oxide, fine and ultrafine zinc oxide, alumina, silica, fuzzy silica (ultrafine anhydrous silicic acid), and titanium mica. Preferred materials include inorganic powders of various sizes and shapes such as fish scale foil, boron nitride, photochromic pigments, synthetic fluorphlogopite, fine particle composite powders, gold, aluminum, and inorganic powders that have been hydrophobized or hydrophilized by treating them with various surface treatment agents such as hydrogen silicone, cyclic hydrogen silicone, or other silanes or titanium coupling agents; organic powders of various sizes and shapes such as starch, cellulose, nylon powder, polyethylene powder, polymethyl methacrylate powder, polystyrene powder, styrene-acrylic acid copolymer resin powder, polyester powder, benzoguanamine resin powder, polyethylene terephthalate-polymethyl methacrylate laminated powder, polyethylene terephthalate-aluminum-epoxy laminated powder, urethane powder, silicone powder, Teflon® powder, and other organic powders and surface-treated powders, as well as organic-inorganic composite powders.Preferred inorganic salts include sodium chloride-containing salts such as table salt, regular salt, rock salt, sea salt, and natural salt; potassium chloride, aluminum chloride, calcium chloride, magnesium chloride, bittern, zinc chloride, and ammonium chloride; sodium sulfate, aluminum sulfate, aluminum potassium sulfate (alum), aluminum ammonium sulfate, barium sulfate, calcium sulfate, potassium sulfate, magnesium sulfate, zinc sulfate, iron sulfate, and copper sulfate; and sodium phosphates such as 1Na, 2Na, and 3Na phosphates, potassium phosphates, calcium phosphates, and magnesium phosphates.

[0036] UV absorbers include benzoic acid-based UV absorbers such as para-aminobenzoic acid, para-aminobenzoic acid monoglycerol ester, N,N-dipropoxypara-aminobenzoate ethyl ester, N,N-diethoxypara-aminobenzoate ethyl ester, N,N-dimethylpara-aminobenzoate ethyl ester, N,N-dimethylpara-aminobenzoate butyl ester, and N,N-dimethylpara-aminobenzoate ethyl ester; anthranilic acid-based UV absorbers such as homomenthyl-N-acetylanthranilate; salicylic acid-based UV absorbers such as salicylic acid and its sodium salt, amyl salicylate, menthyl salicylate, homomenthyl salicylate, octyl salicylate, phenyl salicylate, benzyl salicylate, and p-isopropanolphenyl salicylate; and octyl cinnamate. Cinnamic acid-based UV absorbers such as ethyl-4-isopropyl cinnamate, methyl-2,5-diisopropyl cinnamate, ethyl-2,4-diisopropyl cinnamate, methyl-2,4-diisopropyl cinnamate, propyl-p-methoxy cinnamate, isopropyl-p-methoxy cinnamate, isoamyl-p-methoxy cinnamate, 2-ethylhexyl-p-methoxy cinnamate (octyl paramethoxycinnamate), 2-ethoxyethyl-p-methoxy cinnamate (cinoxate), cyclohexyl-p-methoxy cinnamate, ethyl-α-cyano-β-phenyl cinnamate, 2-ethylhexyl-α-cyano-β-phenyl cinnamate (octocrine), glyceryl mono-2-ethylhexanoyl-diparamethoxy cinnamate, ferulic acid and its derivatives;Benzophenone-based UV absorbers such as 2,4-dihydroxybenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2,2',4,4'-tetrahydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone (oxybenzone-3), 2-hydroxy-4-methoxy-4'-methylbenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-phenylbenzophenone, 2-ethylhexyl-4'-phenylbenzophenone-2-carboxylate, 2-hydroxy-4-n-octoxybenzophenone, 4-hydroxy-3-carboxybenzophenone; 3-(4'-methylbenzylidene)-d,l-camphor, 3-benzylidene-d,l-camphor; 2-phenyl-5-methylbenzoxazole; 2,2'-hydroxy-5-methylphenylbenzotriazole; 2-(2'-hydro Xy-5'-t-octylphenyl)benzotriazole; 2-(2'-hydroxy-5'-methylphenylbenzotriazole; dibenzarazine; dianisioylmethane; 5-(3,3-dimethyl-2-norbornylidene)-3-pentan-2-one; dibenzoylmethane derivatives such as 4-t-butyl methoxydibenzoylmethane; octyltriazone; urocanic acid derivatives such as urocanic acid and ethyl urocanic acid; 2-(2'-hydroxy-5'-methylphenyl)benzotriazole; Preferred materials include benzotriazole (nyl), 1-(3,4-dimethoxyphenyl)-4,4-dimethyl-1,3-pentanedione, 2-ethylhexyl dimethoxybenzylidene dioxoimidazolidinepropionate, and other hydantoin derivatives, phenylbenzimidazol sulfonic acid, terephthalylidene dicamphor sulfonic acid, drometrizole trisiloxane, methyl anthranilate, rutin and its derivatives, and oryzanol and its derivatives.

[0037] Preferred whitening agents include ascorbic acid, ascorbic acid phosphate salts such as sodium ascorbic acid phosphate and magnesium ascorbic acid phosphate, ascorbic acid fatty acid esters such as tetraisopalmitic ascorbic acid, alkyl ascorbic acid ethers such as ethyl ascorbic acid, ascorbic acid glucosides such as ascorbic acid-2-glucoside and their fatty acid esters, ascorbic acid derivatives such as ascorbic acid sulfate and tocopheryl ascorbyl phosphate; kojic acid, ellagic acid, tranexamic acid and its derivatives, ferulic acid and its derivatives, placenta extract, glutathione, oryzanol, butylresorcinol, oil-soluble chamomile extract, oil-soluble licorice extract, willow extract, saxifrage extract, and other plant extracts.

[0038] Preferred anti-inflammatory agents include glycyrrhizic acid and its derivatives, glycyrrhetinic acid derivatives, salicylic acid derivatives, hinokitiol, guaiazulene, allantoin, indomethacin, zinc oxide, hydrocortisone acetate, prednisone, diphedramine hydrochloride, chlorpheniramine maleate; and plant extracts such as peach leaf extract and mugwort leaf extract.

[0039] Preferred hair growth agents, blood circulation promoters, and stimulants include plant extracts and tinctures such as Swertia japonica extract, Capsicum tincture, Ginger tincture, Ginger extract, and Cantharis tincture; capsaicin, nonylic acid valenylamide, gingerol, ichthammol, tannic acid, borneol, cyclandelate, cinnarizine, trazoline, acetylcholine, verapamil, cepharanthine, γ-oryzanol, cepharanthine, vitamin E and derivatives such as tocopherol nicotinate and tocopherol acetate; γ-oryzanol, nicotinic acid and derivatives such as nicotinamide, benzyl nicotinate, inositol hexanicotinate, and nicotinic alcohol; allantoin, photosensitizer 301, photosensitizer 401, capronium chloride, pentadecanoic acid monoglyceride, flavanonol derivatives, stigmasterol or stigmamanol and its glycosides; and minoxidil.

[0040] Other medicinal agents such as anti-aging agents, anti-wrinkle agents, firming agents, cooling agents, warming agents, wound healing promoters, irritation relievers, analgesics, and cell activators include retinols, retinoic acids, retinoic acid tocopheryl; lactic acid, glycolic acid, gluconic acid, fruit acids, salicylic acid and its glycosides and esters and their derivatives; hydroxycapric acid, long-chain α-hydroxy fatty acids, long-chain α-hydroxy fatty acid cholesteryl and other α- or β-hydroxy acids and their derivatives; γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid; carnitine; carnosine; creatine; ceramides, sphingosines; caffeine, xanthine and their derivatives; coenzyme Q10, carotene, lycopene, astaxanthin, lutein, etc. Preferred materials include antioxidants and free radical scavengers; catechins; flavonoids such as quercetin; isoflavones; gallic acid and ester sugar derivatives; polyphenols such as tannins, sesamin, protoanthocyanidins, chlorogenic acid, and apple polyphenols; rutin and glycoside derivatives; hesperidin and glycoside derivatives; lignan glycosides; licorice extract-related substances such as glabridin, glabrene, liquiritin, and isoliquiritin; lactoferrin; gingerol; fragrance substances and their derivatives such as menthol, camphor, and cedrol; capsaicin, vanillin, and their derivatives; insect repellents such as diethyltoluamide; and complexes of physiologically active substances with cyclodextrins.

[0041] Examples of antipruritic agents include diphenhydramine hydrochloride, chlorpheniramine maleate, camphor, and substance-P inhibitors. Examples of exfoliating and dissolving agents include salicylic acid, sulfur, resorcinol, selenium sulfide, and pyridoxine. Examples of antiperspirants include aluminum chlorohydrate, aluminum chloride, zinc oxide, and zinc paraphenolsulfonate. Examples of cooling agents include menthol and methyl salicylate. Examples of astringent agents include citric acid, tartaric acid, lactic acid, potassium aluminum sulfate, and tannic acid. Examples of enzymes include superoxide dismutase, catalase, lysozyme chloride, lipase, papain, pancreatin, and protease. Preferred nucleic acids include ribonucleic acid and its salts, deoxyribonucleic acid and its salts, and adenosine triphosphate disodium.

[0042] Vitamins and their derivatives include vitamin A compounds such as retinol, retinyl acetate, and retinyl palmitate; vitamin B compounds such as thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine dioctanoate, pyridoxine dipalmitate, flavin adenine dinucleotide, cyanocobalamin, folic acid compounds, nicotinic acid compounds such as nicotinamide and benzyl nicotinate, and choline compounds; vitamin C compounds such as ascorbic acid and its sodium salts; vitamin D; vitamin E compounds such as α, β, γ, and δ-tocopherols; other vitamins such as pantothenic acid and biotin; and ascorbic acid phosphate sodium salt and ascorbic acid phosphate magnesium salt. Preferred materials include ascorbic acid phosphate salts, ascorbic acid fatty acid esters such as tetraisopalmitic ascorbate, ascorbyl stearate, ascorbyl palmitate, and ascorbyl dipalmitate, alkyl ascorbic acid ethers such as ethyl ascorbate, ascorbic acid glucosides such as ascorbic acid-2-glucoside and their fatty acid esters, ascorbic acid derivatives such as tocopheryl ascorbyl phosphate; vitamin derivatives such as tocopherol nicotinate, tocopherol acetate, tocopherol linoleate, tocopherol ferulate, and tocopherol phosphate esters, tocotrienols, and various other vitamin derivatives.

[0043] Preferred hormones include estradiol, estrone, ethinylestradiol, cortisone, hydrocortisone, and prednisone.

[0044] Various extracts from plants, animals, and microorganisms include iris extract, angelica extract, Thujopsis dolabrata extract, asparagus extract, avocado extract, Hydrangea macrophylla extract, almond extract, Althaea extract, Arnica extract, apricot extract, apricot kernel extract, Ginkgo biloba extract, Inca extract, fennel extract, turmeric extract, oolong tea extract, bearberry extract, rosehip extract, Echinacea leaf extract, Enmeisou extract, Scutellaria baicalensis extract, Phellodendron amurense extract, Coptis japonica extract, barley extract, Panax ginseng extract, St. John's wort extract, and Lamium album. Grass extract, Ononis extract, Nasturtium officinale extract, Orange extract, Dried seawater, Seaweed extract, Persimmon leaf extract, Arctium moniliforme extract, Hydrolyzed elastin, Hydrolyzed wheat powder, Hydrolyzed silk, Pueraria lobata extract, Chamomile extract, Oil-soluble chamomile extract, Carrot extract, Artemisia capillaris extract, Oat extract, Chloranthus erythrosora extract, Licorice extract, Oil-soluble licorice extract, Kiwi extract, Kelpodium extract, Wood ear mushroom extract, Cinchona extract, Cucumber extract, Paulownia leaf extract, Guanosine, Guava extract, Sophora flavescens extract, Gardenia extract, Sasa veitchii extract Sophora flavescens extract, walnut extract, chestnut extract, grapefruit extract, clematis extract, black rice extract, brown sugar extract, black vinegar, chlorella extract, mulberry extract, gentian extract, Geranium thunbergii extract, black tea extract, yeast extract, Magnolia bark extract, coffee extract, burdock extract, rice extract, rice fermentation extract, rice bran fermentation extract, rice germ oil, comfrey extract, collagen, lingonberry extract, Asarum sieboldii extract, Bupleurum chinense extract, umbilical cord extract, saffron extract, sage extract, soapwort extract, bamboo extract, hawthorn extract, sunflower extract Sodium, Japanese pepper extract, shiitake mushroom extract, rehmannia extract, lithospermum extract, perilla extract, linden extract, meadowsweet extract, jatoba extract, peony extract, ginger extract, calamus root extract, birch extract, white fungus extract, horsetail extract, stevia extract, stevia ferment, iris extract, ivy extract, hawthorn extract, elderflower extract, yarrow extract, peppermint extract, sage extract, mallow extract, chuanxiong extract, swertia japonica extract, mulberry bark extract, rhubarb extract,Soybean extract, jujube extract, thyme extract, dandelion extract, lichen extract, tea extract, clove extract, cogongrass extract, citrus peel extract, tea tree oil, sweet tea extract, chili pepper extract, angelica extract, calendula extract, peach kernel extract, spruce extract, Houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract, wild rose extract, hibiscus extract, Ophiopogon extract, lotus extract, parsley extract, birch extract, honey, witch hazel extract, parietaria extract, Isodon japonicus extract, bisabolol, cypress extract, bifidobacteria extract, loquat extract, coltsfoot extract, butterbur extract, Poria cocos extract, butcher's broom extract, grape extract Examples of extracts include grape seed extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, cypress extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, lemon balm extract, seaweed extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, lily extract, coix seed extract, mugwort extract, lavender extract, green tea extract, eggshell membrane extract, apple extract, rooibos tea extract, reishi mushroom extract, lettuce extract, lemon extract, forsythia extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, royal jelly extract, and burnet extract.

[0045] The fragrance ingredients include acetylcedrene, amyl cinnamaldehyde, allyl amyl glycolate, β-ionone, iso-esuper, isobutylquinoline, iris oil, ylone, indole, ylang-ylang oil, undecanal, undecenal, γ-undecalactone, estragol, eugenol, oakmoss, opoponax resinoid, orange oil, eugenol, aurantiol, galacsolid, carvacrol, L-carbone, camphor, canone, carrot seed oil, clove oil, methyl cinnamate, geraniol, geranyl nitrile, isobornyl acetate, geranyl acetate, dimethylbenzylcarbinyl acetate, styraryl acetate, cedyl acetate, terepinel acetate, and pt-butylcyclohexyl acetate. Lu, vetiveryl acetate, benzyl acetate, linalyl acetate, isopentyl salicylate, benzyl salicylate, sandalwood oil, santalol, cyclamenaldehyde, cyclopentadecanol, methyl dihydrojasmonate, dihydromyrcenol, jasmine absolute, jasmine lactone, cis-jasmone, citral, citronenol, citronellal, cinnamon bark oil, 1,8-cineole, cinnamaldehyde, styrax resinoid, cedarwood oil, cedrene, cedrol, celery seed oil, thyme oil, damascone, damascenone, thymol, tuberose absolute, decanal, decalactone, terpineol, γ-terpinene, tripral, nerol, nonanal, 2,6-Nonadienol, Nonalactone, Patchouli Alcohol, Vanilla Absolute, Vanillin, Basil Oil, Patchouli Oil, Hydroxycitronellal, α-Pinene, Piperitone, Phenethyl Alcohol, Phenylacetaldehyde, Petitgrain Oil, Hexyl Cinnamaldehyde, cis-3-Hexenol, Peruvian Balsam, Vetiver Oil, Vetiverol, Peppermint Oil, Pepper Oil, Heliotropin, Bergamot Oil, Benzyl Benzoate Preferred examples include synthetic and natural fragrances such as benzoate, borneol, myrrhizinoid, musk ketone, methylnonylacetaldehyde, γ-methylionone, menthol, L-menthol, L-menthone, eucalyptus oil, β-ionone, lime oil, lavender oil, D-limonene, linalool, liral, lilial, lemon oil, rose absolute, rose oxide, rose oil, rosemary oil, various essential oils, and various blended fragrances.

[0046] As pigments, colorants, dyes, and dyes, they include Brown No. 201, Black No. 401, Purple No. 201, Purple No. 401, Blue No. 1, Blue No. 2, Blue No. 201, Blue No. 202, Blue No. 203, Blue No. 204, Blue No. 205, Blue No. 403, Blue No. 404, Green No. 201, Green No. 202, Green No. 204, Green No. 205, Green No. 3, Green No. 401, Green No. 402, and Red. Color No. 102, Red No. 104-1, Red No. 105-1, Red No. 106, Red No. 2, Red No. 201, Red No. 202, Red No. 203, Red No. 204, Red No. 205, Red No. 206, Red No. 207, Red No. 208, Red No. 213, Red No. 214, Red No. 215, Red No. 218, Red No. 219, Red No. 220, Red No. 221, Red No. 223, Red No. 22 No. 5, Red No. 226, Red No. 227, Red No. 228, Red No. 230-1, Red No. 230-2, Red No. 231, Red No. 232, Red No. 3, Red No. 401, Red No. 404, Red Color No. 405, Red No. 501, Red No. 502, Red No. 503, Red No. 504, Red No. 505, Red No. 506, Orange No. 201, Orange No. 203, Orange No. 204, Orange No. 205, Orange No. 206, Orange No. 207, Orange No. 401, Orange No. 402, Orange No. 403, Yellow No. 201, Yellow No. 202-1, Yellow No. 202-2, Yellow No. 203, Yellow No. 204, Yellow Legal dyes such as No. 205, Yellow No. 4, Yellow No. 401, Yellow No. 402, Yellow No. 403-1, Yellow No. 404, Yellow No. 405, Yellow No. 406, Yellow No. 407, Yellow No. 5; Acid Other acidic dyes such as Red 14; Basic dyes such as Arianor Sienna Brown, Arianor Madder Red, Arianor Steel Blue, Arianor Straw Yellow; HC Yellow 2, HC Yellow 5, HC Red 3, 4-hydroxypropylamino-3-nitrophenol, N,N'-bis(2-hydroxyethyl)-2-nitro-p- Nitro dyes such as phenylenediamine, HC Blue 2, and Basic Blue 26; disperse dyes; inorganic white pigments such as titanium dioxide and zinc oxide; inorganic red pigments such as iron oxide (red iron oxide) and iron titanate; inorganic brown pigments such as γ-iron oxide; inorganic yellow pigments such as yellow iron oxide and ochre; inorganic black pigments such as black iron oxide and lower titanium oxide; inorganic purple pigments such as mango violet and cobalt violet; inorganic green pigments such as chromium oxide, chromium hydroxide, and cobalt titanate; inorganic blue pigments such as ultramarine and Prussian blue; titanium dioxide coated mica, titanium dioxide coated oxy Pearl pigments such as bismuth chloride, titanium dioxide-coated talc, colored titanium dioxide-coated mica, bismuth oxychloride, and fish scale foil; metal powder pigments such as aluminum powder, copper powder, and gold; surface-treated inorganic and metal powder pigments; Red 201, Red 202, Red 204, Red 205, Red 220, Red 226, Red 228, Red 405, Orange 203, Orange 204, Yellow 205, Yellow 401, Blue 404, Red 3, Red 104, Red 106, Red 22 Organic pigments such as zirconium, barium, or aluminum lake, including No. 7, Red No. 230, Red No. 401, Red No. 505, Orange No. 205, Yellow No. 4, Yellow No. 5, Yellow No. 202, Yellow No. 203, Green No. 3, Blue No. 1, etc.; surface-treated organic pigments; anthraquinones such as astaxanthin and alizarin, anthocyanidins, β-carotene, catenal, capsanthin, chalcone, calsamine, quercetin, crocin, chlorophyll, curcumin, cochineal, shikonin, and other naphthoquinones, bixy Natural pigments and dyes such as flavonoids, betacyanidin, henna, hemoglobin, lycopene, riboflavin, and rutin; oxidative dye intermediates and couplers such as p-phenylenediamine, toluene-2,5-diamine, o-,m-, or p-aminophenol, m-phenylenediamine, 5-amino-2-methylphenol, resorcinol, 1-naphthol, 2,6-diaminopyridine, and their salts; auto-oxidative dyes such as indoline; and dihydroxyacetone are preferred.

[0047] Other ingredients may include those listed in the Cosmetic Ingredient Standards, Cosmetic Ingredient Formulation Standards, Japan Cosmetic Industry Association Ingredient Labeling Name List, INCI Dictionary (The International Cosmetic Ingredient Dictionary and Handbook), Quasi-drug Ingredient Standards, Japanese Pharmacopoeia, Pharmaceutical Additives Standards, and the Food Additives Compendium, as well as known cosmetic ingredients and pharmaceutical ingredients. Such ingredients may be included in cosmetics or topical skin preparations to the extent that the expected effects of Suizenji-nori-derived sulfated polysaccharides (specifically, skin surface barrier film formation effects) can be obtained.

[0048] Cosmetics or topical skin preparations may contain an effective amount of sulfated polysaccharides derived from Suizenji-nori. "Effective amount" refers to the amount of Suizenji-nori-derived sulfated polysaccharides that is effective in exerting the expected effects (specifically, the skin surface barrier film formation effect). The effective amount may vary depending on the age, sex, and weight of the user. In cosmetics or topical skin preparations, the effective amount of sulfated polysaccharides derived from Suizenji-nori is, for example, 0.010% by mass or more as the content of sulfated polysaccharides derived from Suizenji-nori in the cosmetic or topical skin preparation, preferably 0.010% by mass to 1.000% by mass, more preferably 0.030% by mass to 0.800% by mass, even more preferably 0.050% by mass to 0.500% by mass, particularly preferably 0.060% by mass to 0.300% by mass, quite preferably 0.070% by mass to 0.250% by mass, very preferably 0.075% by mass to 0.200% by mass, and most preferably 0.080% by mass to 0.150% by mass.

[0049] The dosage form of cosmetics or topical skin preparations is not particularly limited, but examples include ampoules, capsules, pills, tablets, powders, granules, solids, liquids, gels, bubbles, emulsions, sheets, mists, sprays, etc. Cosmetics or topical skin preparations may be pharmaceuticals, quasi-drugs, or cosmetics.

[0050] While there are no particular limitations on the uses of cosmetics, examples include basic cosmetics such as lotions, emulsions, creams, ointments, oils, and masks; skin cleansing products such as facial cleansers and body washes; massage cosmetics such as massage agents; cleansing cosmetics such as cleansing agents; hair removal cosmetics such as depilatory agents and hair removal agents; shaving cosmetics such as shaving creams, aftershave lotions, pre-shave lotions, and shaving creams; makeup cosmetics such as foundations, lipsticks, blushes, eyeshadows, eyeliners, and mascaras; and other topical cosmetics such as perfumes, nail care products, nail polish, nail polish removers, poultices, plasters, tapes, sheets, patches, and aerosols. Cosmetics or topical skin preparations may also be preparations for the scalp and / or hair. Examples of preparations for the scalp and / or hair include shampoos, conditioners, hair treatments, pre-hair treatments, permanent solutions, hair dyes, hair styling products, hair tonics, hair growth and nourishing products, scalp poultices, scalp plasters, scalp tapes, scalp sheets, scalp patches, hair or scalp aerosols, etc. Furthermore, the cosmetic or topical skin preparation may also be a bath additive used by adding it to bathwater. The cosmetic or topical skin preparation of the present invention can also be used as an antiperspirant, deodorant, odor control agent, antiperspirant, sanitary product, sanitary cotton, wet wipes, toothpaste, mouth freshener, mouthwash, dishwashing detergent, etc.

[0051] <Skin barrier function improving agent> The skin barrier function improving agent of the present invention contains sulfated polysaccharides derived from Suizenji-nori and is an agent that improves, repairs, or enhances the skin's naturally occurring barrier function. In this specification, these effects are collectively referred to as "barrier function improvement." In the skin barrier function improving agent, Suizenji-nori-derived sulfated polysaccharides are contained as an active ingredient. Skin barrier function improving agents aim to strengthen the skin's barrier function and protect it from external stimuli and pollutants. This strengthens the skin's barrier function, reduces the occurrence of skin problems, and helps maintain healthy skin.

[0052] The sulfated polysaccharides derived from Suizenji-nori contained in the skin barrier function improving agent are as described above. The skin cells targeted by the skin barrier function improving agent of the present invention may be cells present in the epidermis or cells present in the dermis, but when the skin barrier function improving agent of the present invention is used in cosmetics, it is particularly preferable to target the epidermis. Examples of cells present in the epidermis include granule cells, spinous cells, Langerhans cells, and basal cells. Examples of cells present in the dermis include fibroblasts.

[0053] The skin barrier function improving agent of the present invention may be used as is, but may also be incorporated into cosmetics, topical skin preparations, quasi-drugs, pharmaceuticals, etc., for the purpose of imparting a skin barrier function improving effect. In particular, its use in cosmetics or topical skin preparations is preferred. When incorporated into cosmetics, etc., other components may be mixed in as needed to form the formulation, and the above-mentioned components are examples of such other components. The skin barrier function improving agent of the present invention is preferably applied to healthy skin, preferably to healthy human skin, and more preferably used in cosmetic applications on healthy human skin.

[0054] <Skincare Method> The skincare method of the present invention involves applying the above-mentioned skin surface barrier film-forming agent or skin barrier function improving agent to healthy skin. This method is a non-therapeutic method, excluding methods for treating humans, and is preferably applied to the skin of a healthy person, and more preferably used for cosmetic purposes on the skin of a healthy person. The skincare method of the present invention is implemented with the aim of enhancing the overall barrier function of the skin, improving the skin's resistance to external stress, or enhancing the skin's anti-fouling properties. As a result of applying this method, it is possible to protect the skin from harmful substances in the air, avoid skin problems, and maintain healthy skin. The skin problems anticipated by this method are very minor, usually temporary, and often heal naturally, so they are not considered diseases or illnesses. In addition, since these problems rarely cause serious health problems, the response to these problems is non-therapeutic rather than therapeutic, and falls within the realm of improvement that can be achieved with daily skincare.

[0055] The skin surface barrier film-forming agent or skin barrier function improving agent used in the method of the present invention may be used as is, or it may be used in a form incorporated into cosmetics, topical skin preparations, quasi-drugs, pharmaceuticals, etc. The method of application to the skin surface is not particularly limited and can be done by applying, spraying, or patching to the target area, but application is preferred. Application may be done with fingers, or using application materials such as cotton, gauze, puffs, or tissue paper. The detailed usage and dosage are not particularly limited and can be determined as appropriate considering the effective amount, but for example, it can be 1 to 5 times, 1 to 3 times, 1 to 2 times, or once per day.

[0056] In another embodiment, the present invention provides sulfated polysaccharides derived from Suizenji-nori for forming a skin surface barrier film or improving skin barrier function, and the use of sulfated polysaccharides derived from Suizenji-nori for forming a skin surface barrier film or improving skin barrier function. [Examples]

[0057] The present invention will be specifically described below with reference to examples, but the present invention is not limited to these examples.

[0058] [Examples 1-2, Comparative Example 1] (Example 1: Evaluation test of skin barrier properties of polysaccharide sulfate) The skin barrier function-enhancing effect of sulfated polysaccharides derived from Suizenji-nori was evaluated using the following procedure. The skin model used in the study was REVskin, a 4D full-thickness skin model manufactured by REVIVO Biosystems Pte Ltd., with four tissue samples used for each condition. The following test samples were applied to the 4D full-thickness skin model to evaluate skin barrier function. As the sulfated polysaccharide derived from Suizenji-nori, "Sakuranex" (registered trademark) manufactured by the DIC Group was used. Test sample: Sakuranex 0.05% Sakuranex 0.1% • Phosphate-buffered saline (PBS)

[0059] The test method involved uniformly applying the test sample to the surface of a 4D full-thickness skin model. After a 1-hour incubation, Lucifer Yellow (1 mM), a cell staining reagent, was applied to the surface of the skin model. After another 1-hour incubation, frozen sections were prepared, and the diffusion of Lucifer Yellow in the skin cross-section was observed using a fluorescence microscope. Image analysis software ImageJ was used to quantify the diffusion area. The test results showed that the fluorescence dye diffusion area (μm²) of Lucifer Yellow in each test sample was as follows: 2 ) is shown below.

[0060] [Table 1]

[0061] From the results above, it was confirmed that the agent according to the present invention enhances the skin's barrier function and protects the skin from external stimuli. It is believed that through this action, the agent according to the present invention improves the skin's resistance to external stress and enhances the skin's anti-fouling properties.

Claims

1. A skin surface barrier film-forming agent containing sulfated polysaccharides derived from Suizenji-nori as an active ingredient.

2. A skin barrier function improving agent containing sulfated polysaccharides derived from Suizenji-nori as an active ingredient.

3. A skincare method for enhancing the skin's barrier function, improving the skin's resistance to external stress, and improving the skin's resistance to dirt, A skin care method comprising applying the skin surface barrier film-forming agent described in claim 1 or the skin barrier function improving agent described in claim 2 to a healthy skin surface.

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